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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcoI | YGGCCR | 1 cut(s) | 10 |
| AfaI | GTAC | 1 cut(s) | 79 |
| AhlI | ACTAGT | 1 cut(s) | 71 |
| AoxI | GGCC | 1 cut(s) | 10 |
| AseI | ATTAAT | 1 cut(s) | 96 |
| BbsI | GAAGAC | 1 cut(s) | 28 |
| BccI | CCATC | 1 cut(s) | 71 |
| BcuI | ACTAGT | 1 cut(s) | 71 |
| BfaI | CTAG | 1 cut(s) | 72 |
| BmcAI | AGTACT | 1 cut(s) | 79 |
| BpiI | GAAGAC | 1 cut(s) | 28 |
| Bse3DI | GCAATG | 1 cut(s) | 119 |
| BseMI | GCAATG | 1 cut(s) | 119 |
| BshFI | GGCC | 1 cut(s) | 12 |
| BsnI | GGCC | 1 cut(s) | 12 |
| BspANI | GGCC | 1 cut(s) | 12 |
| BsrDI | GCAATG | 1 cut(s) | 119 |
| BstDEI | CTNAG | 1 cut(s) | 40 |
| BstV2I | GAAGAC | 1 cut(s) | 28 |
| BsuRI | GGCC | 1 cut(s) | 12 |
| Csp6I | GTAC | 1 cut(s) | 78 |
| CviJI | RGCY | 1 cut(s) | 12 |
| CviKI_1 | RGCY | 1 cut(s) | 12 |
| CviQI | GTAC | 1 cut(s) | 78 |
| DdeI | CTNAG | 1 cut(s) | 40 |
| EaeI | YGGCCR | 1 cut(s) | 10 |
| FspBI | CTAG | 1 cut(s) | 72 |
| HaeIII | GGCC | 1 cut(s) | 12 |
| HpyF3I | CTNAG | 1 cut(s) | 40 |
| LpnPI | CCDG | 2 cut(s) | 127, 171 |
| MaeI | CTAG | 1 cut(s) | 72 |
| MaeIII | GTNAC | 2 cut(s) | 85, 145 |
| MboII | GAAGA | 1 cut(s) | 28 |
| MluCI | AATT | 2 cut(s) | 97, 201 |
| MnlI | CCTC | 2 cut(s) | 28, 120 |
| MseI | TTAA | 4 cut(s) | 23, 96, 138, 214 |
| NmuCI | GTSAC | 1 cut(s) | 85 |
| PshBI | ATTAAT | 1 cut(s) | 96 |
| RsaI | GTAC | 1 cut(s) | 79 |
| RsaNI | GTAC | 1 cut(s) | 78 |
| SaqAI | TTAA | 4 cut(s) | 23, 96, 138, 214 |
| ScaI | AGTACT | 1 cut(s) | 79 |
| SetI | ASST | 2 cut(s) | 20, 112 |
| SgeI | CNNG | 8 cut(s) | 31, 41, 84, 93, 97, 102, 126, 170 |
| SpeI | ACTAGT | 1 cut(s) | 71 |
| Sse9I | AATT | 2 cut(s) | 97, 201 |
| SspI | AATATT | 1 cut(s) | 165 |
| SspMI | CTAG | 1 cut(s) | 72 |
| TasI | AATT | 2 cut(s) | 97, 201 |
| TatI | WGTACW | 1 cut(s) | 77 |
| Tru1I | TTAA | 4 cut(s) | 23, 96, 138, 214 |
| Tru9I | TTAA | 4 cut(s) | 23, 96, 138, 214 |
| TseFI | GTSAC | 1 cut(s) | 85 |
| Tsp45I | GTSAC | 1 cut(s) | 85 |
| TspDTI | ATGAA | 1 cut(s) | 108 |
| VspI | ATTAAT | 1 cut(s) | 96 |
| XspI | CTAG | 1 cut(s) | 72 |
| ZrmI | AGTACT | 1 cut(s) | 79 |