FvH4_6g43792

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000768
Physical Location & Seq
Reverse (-)
33842048 .. 33844939
2892 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g43792.t1

Sequence Viewer

Length: 1500 bp
ATGTCTACCCCAATCTTGGCCAGCAACGGTAGCACTGCTACTGAAACTTCATATCTGGATGTTCAAAGACGGACAGCAGATTACAAGCCCAGCATCTGGAGTTACGAGTTTCTTCAATCGTTACAGGAAGAACTATACGAAGATGATAGGTGGAAGTCGATGGAGGAGTGTGTGAAACATATGCTTGGCTATGATAATGATGCGCAGTCAGTAGAGACTAAACTTGAATTGATTGATGACATCCAACGATTGGGTTTGGGACACCGATTCCAGGCCAGTATAATAGGAACCCTTCATAGAATTATGAAGAATCTTGACCAAGTAACAGACGAGGATACCCAAAGTCTCCACCTCACTGCTCTCAGCTTCAGACTCCTTCGACAGCATGGTTTTCAAGTCTCCCAAGATGTCTTCAAGAATTTCACGGACTGCGACGGCAGCTTCAAGGATTGCCTTTGCAAAGATGTCAAAGGAATGCTGAGTCTGTACGAAGCTTCATTCCTTGGTTTTGAAGGAGAAACCCTCTTGGATGAGGCCCTTGCATTCACCAGCATGCACCTAAAAAACCTCAGCTGCAGGTTACTTGATACTCACGGCAGAAATGTATTAGAGCAAGTGAGTCATGCATTGGAGATGCCATTGCACCACAGAATGCAAAGATTAGAAGCTAGATGGTATATTGAGGTATATAATAAAAGGCAAGATGCAAATCAGATGCTACTTGAATTTGCCAAGCTAGATTACGATGTGGTGCAACAGACATACCAAAGTGATCTTAAAGACTTATCAAGATGGTGGAAGGATATGGGGTTAGCAAAGAAGTTGTCCTTCAGCAGAGACAGATTGATGGAGTGCTTCTTTTGGTCAGTTGGTATAGTGTCTGAACCTCAATTCAGCAATCTCAGAAAAGGGATAACTAAAGTCAGTGTTCTAATATGCACAATTGATGATGTGTATGATGTTTATGGTACTCTGGATGAACTAAAGTTATTCACATCTGTTGTTGAAAGATGGGATGTGAATGCTGTGGAAATTCTACAAGACTACCAGCTGAAGCTCTCTTTCCTTGCTCTCTTCAACACTGTGAATGAAATGGCATATGAAACTTTGAAAGACCAAGGAGTGGACGTCCTTCCTTACTTGACAAAAGCTTGGACTGATATGTGCAAAGCTTTCTTAACGGAGGCAGAGTGGCGTTACAACAATTACACACCCACATTTGAAGAATATATTGCCAATGCATGGATATCAGTGTCCGGGGTGCTCCTATTAGTGCATACATATTTTCTACTGAATCAAAATATCTCAGATGAAGCACTTGAATGCTTAGTGAATCACCATGATCTCTTGCGCTGGCCGTCTCTTATTTTTCGGCTCTCAAACGATTTGGCTACTTCCGAGGCAGAAATAGAGAGAGGTGAAACTGCAACTTCAATATCCTGCATCAATCGTGGAAGTAGTGATGTTGATGTGATAGGCATCTGCTCGCCAATTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko01110 map00900 map01110
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

500

Amino Acids

57.65

Weight (kDa)

4.87

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 33 - 210 1.6e-50 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 267 - 482 4.7e-78 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 307 - 484 2.9e-49 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1131
Acc16I TGCGCA 1 cut(s) 204
Acc36I ACCTGC 1 cut(s) 567
AccB7I CCANNNNNTGG 4 cut(s) 96, 250, 1123, 1242
AccI GTMKAC 1 cut(s) 5
AcoI YGGCCR 2 cut(s) 18, 1355
AcsI RAATTY 3 cut(s) 418, 725, 1032
AcuI CTGAAG 3 cut(s) 352, 814, 1073
AcyI GRCGYC 1 cut(s) 1128
AfaI GTAC 2 cut(s) 488, 970
AfiI CCNNNNNNNGG 6 cut(s) 16, 96, 250, 271, 1123, 1242
AjnI CCWGG 1 cut(s) 270
Alw21I GWGCWC 1 cut(s) 1266
Alw26I GTCTC 5 cut(s) 209, 350, 403, 831, 1365
AlwNI CAGNNNCTG 1 cut(s) 96
AoxI GGCC 4 cut(s) 18, 273, 534, 1355
ApeKI GCWGC 2 cut(s) 438, 573
ApoI RAATTY 3 cut(s) 418, 725, 1032
ArsI GACNNNNNNTTYG 2 cut(s) 809, 841
AspLEI GCGC 2 cut(s) 205, 1353
AspS9I GGNCC 1 cut(s) 535
AsuC2I CCSGG 1 cut(s) 1258
AsuHPI GGTGA 3 cut(s) 538, 1328, 1430
BalI TGGCCA 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 403
Bbv12I GWGCWC 1 cut(s) 1266
BbvCI CCTCAGC 1 cut(s) 569
BbvI GCAGC 2 cut(s) 450, 560
BccI CCATC 5 cut(s) 154, 666, 786, 841, 1005
BceAI ACGGC 3 cut(s) 451, 610, 1342
BciT130I CCWGG 1 cut(s) 272
BciVI GTATCC 1 cut(s) 328
BcnI CCSGG 1 cut(s) 1258
BcoDI GTCTC 5 cut(s) 209, 350, 403, 831, 1365
BfaI CTAG 2 cut(s) 669, 737
BfmI CTRYAG 1 cut(s) 574
BfuAI ACCTGC 1 cut(s) 567
BfuI GTATCC 1 cut(s) 328
BisI GCNGC 2 cut(s) 439, 574
BlsI GCNGC 2 cut(s) 440, 575
Bme1390I CCNGG 2 cut(s) 272, 1258
BmgT120I GGNCC 1 cut(s) 535
BmiI GGNNCC 1 cut(s) 289
BmrFI CCNGG 2 cut(s) 272, 1258
BmsI GCATC 7 cut(s) 102, 190, 624, 694, 705, 1452, 1488
BpiI GAAGAC 1 cut(s) 403
BplI GAGNNNNNCTC 2 cut(s) 507, 539
BpmI CTGGAG 1 cut(s) 118
Bpu10I CCTNAGC 1 cut(s) 569
BpuMI CCSGG 1 cut(s) 1258
BsaBI GATNNNNATC 2 cut(s) 708, 1478
BsaHI GRCGYC 1 cut(s) 1128
BsaJI CCNNGG 4 cut(s) 502, 1117, 1257, 1398
BsaXI ACNNNNNCTCC 4 cut(s) 1113, 1143, 1175, 1205
Bsc4I CCNNNNNNNGG 6 cut(s) 16, 96, 250, 271, 1123, 1242
Bse1I ACTGG 1 cut(s) 276
Bse3DI GCAATG 1 cut(s) 638
Bse8I GATNNNNATC 2 cut(s) 708, 1478
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 4 cut(s) 502, 1117, 1257, 1398
BseGI GGATG 5 cut(s) 64, 240, 535, 982, 1021
BseJI GATNNNNATC 2 cut(s) 708, 1478
BseLI CCNNNNNNNGG 6 cut(s) 16, 96, 250, 271, 1123, 1242
BseMI GCAATG 1 cut(s) 638
BseMII CTCAG 5 cut(s) 376, 470, 583, 916, 1320
BseNI ACTGG 1 cut(s) 276
BseRI GAGGAG 1 cut(s) 179
BseXI GCAGC 2 cut(s) 450, 560
BseYI CCCAGC 1 cut(s) 89
BshFI GGCC 4 cut(s) 20, 275, 536, 1357
BsiHKAI GWGCWC 1 cut(s) 1266
BsiSI CCGG 1 cut(s) 1257
BslFI GGGAC 1 cut(s) 273
BslI CCNNNNNNNGG 6 cut(s) 16, 96, 250, 271, 1123, 1242
BsmAI GTCTC 5 cut(s) 209, 350, 403, 831, 1365
BsmBI CGTCTC 1 cut(s) 1365
BsmFI GGGAC 1 cut(s) 273
BsmI GAATGC 5 cut(s) 480, 542, 657, 1027, 1328
BsnI GGCC 4 cut(s) 20, 275, 536, 1357
Bsp1286I GDGCHC 1 cut(s) 1266
Bsp143I GATC 2 cut(s) 772, 1342
BspANI GGCC 4 cut(s) 20, 275, 536, 1357
BspCNI CTCAG 5 cut(s) 375, 471, 582, 915, 1319
BspLI GGNNCC 1 cut(s) 289
BspMAI CTGCAG 1 cut(s) 578
BspMI ACCTGC 1 cut(s) 567
BsrDI GCAATG 1 cut(s) 638
BsrI ACTGG 1 cut(s) 276
BssECI CCNNGG 4 cut(s) 502, 1117, 1257, 1398
BssMI GATC 2 cut(s) 772, 1342
BssNI GRCGYC 1 cut(s) 1128
BssT1I CCWWGG 2 cut(s) 502, 1117
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 2 cut(s) 29, 1084
Bst6I CTCTTC 1 cut(s) 1079
BstACI GRCGYC 1 cut(s) 1128
BstC8I GCNNGC 4 cut(s) 22, 554, 1355, 1487
BstDEI CTNAG 6 cut(s) 362, 479, 569, 902, 1306, 1327
BstF5I GGATG 5 cut(s) 64, 240, 535, 982, 1021
BstHHI GCGC 2 cut(s) 205, 1353
BstKTI GATC 2 cut(s) 775, 1345
BstMAI GTCTC 5 cut(s) 209, 350, 403, 831, 1365
BstMBI GATC 2 cut(s) 772, 1342
BstMWI GCNNNNNNNGC 2 cut(s) 30, 438
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 556
BstSCI CCNGG 2 cut(s) 270, 1256
BstSFI CTRYAG 1 cut(s) 574
BstV1I GCAGC 2 cut(s) 450, 560
BstV2I GAAGAC 1 cut(s) 403
BsuI GTATCC 1 cut(s) 328
BsuRI GGCC 4 cut(s) 20, 275, 536, 1357
BtsCI GGATG 5 cut(s) 64, 240, 535, 982, 1021
BtsI GCAGTG 2 cut(s) 33, 354
BtsIMutI CAGTG 5 cut(s) 33, 354, 931, 1080, 1257
BveI ACCTGC 1 cut(s) 567
Cac8I GCNNGC 4 cut(s) 22, 554, 1355, 1487
CaiI CAGNNNCTG 1 cut(s) 96
CfoI GCGC 2 cut(s) 205, 1353
Cfr13I GGNCC 1 cut(s) 535
Csp6I GTAC 2 cut(s) 487, 969
CviAII CATG 5 cut(s) 386, 553, 623, 1242, 1340
CviQI GTAC 2 cut(s) 487, 969
DdeI CTNAG 6 cut(s) 362, 479, 569, 902, 1306, 1327
DpnI GATC 2 cut(s) 774, 1344
DpnII GATC 2 cut(s) 772, 1342
EaeI YGGCCR 2 cut(s) 18, 1355
Eam1104I CTCTTC 1 cut(s) 1079
EarI CTCTTC 1 cut(s) 1079
Eco130I CCWWGG 2 cut(s) 502, 1117
Eco32I GATATC 1 cut(s) 1248
Eco57I CTGAAG 3 cut(s) 352, 814, 1073
EcoO109I RGGNCCY 1 cut(s) 535
EcoRII CCWGG 1 cut(s) 270
EcoRV GATATC 1 cut(s) 1248
EcoT14I CCWWGG 2 cut(s) 502, 1117
EcoT22I ATGCAT 2 cut(s) 628, 1243
ErhI CCWWGG 2 cut(s) 502, 1117
Esp3I CGTCTC 1 cut(s) 1365
FaeI CATG 5 cut(s) 389, 556, 626, 1245, 1343
FalI AAGNNNNNCTT 2 cut(s) 812, 844
FaqI GGGAC 1 cut(s) 273
FatI CATG 5 cut(s) 385, 552, 622, 1241, 1339
FauNDI CATATG 2 cut(s) 180, 1099
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 439, 574
FokI GGATG 5 cut(s) 71, 227, 542, 989, 1028
Fsp4HI GCNGC 2 cut(s) 439, 574
FspBI CTAG 2 cut(s) 669, 737
FspI TGCGCA 1 cut(s) 204
GlaI GCGC 2 cut(s) 204, 1352
GluI GCNGC 2 cut(s) 439, 574
GsaI CCCAGC 1 cut(s) 93
GsuI CTGGAG 1 cut(s) 118
HaeIII GGCC 4 cut(s) 20, 275, 536, 1357
HapII CCGG 1 cut(s) 1257
HhaI GCGC 2 cut(s) 205, 1353
Hin1I GRCGYC 1 cut(s) 1128
Hin1II CATG 5 cut(s) 389, 556, 626, 1245, 1343
Hin6I GCGC 2 cut(s) 203, 1351
HinP1I GCGC 2 cut(s) 203, 1351
HindIII AAGCTT 3 cut(s) 492, 1149, 1170
HinfI GANTC 7 cut(s) 267, 310, 372, 481, 619, 1294, 1333
HpaII CCGG 1 cut(s) 1257
HphI GGTGA 3 cut(s) 538, 1328, 1430
Hpy166II GTNNAC 2 cut(s) 6, 1126
Hpy188I TCNGA 6 cut(s) 371, 714, 883, 905, 1309, 1399
Hpy188III TCNNGA 6 cut(s) 56, 97, 314, 415, 789, 974
Hpy8I GTNNAC 2 cut(s) 6, 1126
Hpy99I CGWCG 1 cut(s) 437
HpyAV CCTTC 6 cut(s) 302, 386, 506, 793, 838, 1142
HpyCH4III ACNGT 2 cut(s) 29, 1084
HpyCH4IV ACGT 1 cut(s) 1128
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 438
HpyF3I CTNAG 6 cut(s) 362, 479, 569, 902, 1306, 1327
HpySE526I ACGT 1 cut(s) 1128
Hsp92I GRCGYC 1 cut(s) 1128
Hsp92II CATG 5 cut(s) 389, 556, 626, 1245, 1343
HspAI GCGC 2 cut(s) 203, 1351
Kzo9I GATC 2 cut(s) 772, 1342
LmnI GCTCC 1 cut(s) 1269
Lsp1109I GCAGC 2 cut(s) 450, 560
LweI GCATC 7 cut(s) 102, 190, 624, 694, 705, 1452, 1488
MaeI CTAG 2 cut(s) 669, 737
MaeII ACGT 1 cut(s) 1128
MaeIII GTNAC 5 cut(s) 101, 120, 322, 579, 1196
MalI GATC 2 cut(s) 774, 1344
MboI GATC 2 cut(s) 772, 1342
MboII GAAGA 7 cut(s) 104, 140, 152, 319, 403, 1066, 1235
MfeI CAATTG 1 cut(s) 942
MhlI GDGCHC 1 cut(s) 1266
MlsI TGGCCA 1 cut(s) 20
MluCI AATT 9 cut(s) 227, 300, 418, 725, 890, 942, 1032, 1204, 1491
MluNI TGGCCA 1 cut(s) 20
MlyI GAGTC 3 cut(s) 366, 490, 628
MmeI TCCRAC 1 cut(s) 268
Mox20I TGGCCA 1 cut(s) 20
Mph1103I ATGCAT 2 cut(s) 628, 1243
MscI TGGCCA 1 cut(s) 20
MseI TTAA 2 cut(s) 777, 1178
MslI CAYNNNNRTG 2 cut(s) 551, 1321
Msp20I TGGCCA 1 cut(s) 20
MspA1I CMGCKG 2 cut(s) 573, 1051
MspI CCGG 1 cut(s) 1257
MspR9I CCNGG 2 cut(s) 272, 1258
MunI CAATTG 1 cut(s) 942
Mva1269I GAATGC 5 cut(s) 480, 542, 657, 1027, 1328
MvaI CCWGG 1 cut(s) 272
MwoI GCNNNNNNNGC 2 cut(s) 30, 438
NciI CCSGG 1 cut(s) 1258
NdeI CATATG 2 cut(s) 180, 1099
NdeII GATC 2 cut(s) 772, 1342
NlaIII CATG 5 cut(s) 389, 556, 626, 1245, 1343
NlaIV GGNNCC 1 cut(s) 289
NsbI TGCGCA 1 cut(s) 204
NsiI ATGCAT 2 cut(s) 628, 1243
NspI RCATGY 1 cut(s) 556
PaeI GCATGC 1 cut(s) 556
PctI GAATGC 5 cut(s) 480, 542, 657, 1027, 1328
PfeI GAWTC 4 cut(s) 267, 310, 1294, 1333
PflMI CCANNNNNTGG 4 cut(s) 96, 250, 1123, 1242
PkrI GCNGC 2 cut(s) 440, 575
PleI GAGTC 3 cut(s) 366, 489, 627
PpsI GAGTC 3 cut(s) 366, 489, 627
Psp6I CCWGG 1 cut(s) 270
PspFI CCCAGC 1 cut(s) 89
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 1 cut(s) 289
PspPI GGNCC 1 cut(s) 535
PstI CTGCAG 1 cut(s) 578
PstNI CAGNNNCTG 1 cut(s) 96
PvuII CAGCTG 2 cut(s) 573, 1051
RsaI GTAC 2 cut(s) 488, 970
RsaNI GTAC 2 cut(s) 487, 969
RseI CAYNNNNRTG 2 cut(s) 551, 1321
SaqAI TTAA 2 cut(s) 777, 1178
SatI GCNGC 2 cut(s) 439, 574
Sau3AI GATC 2 cut(s) 772, 1342
Sau96I GGNCC 1 cut(s) 535
SchI GAGTC 3 cut(s) 366, 490, 628
ScrFI CCNGG 2 cut(s) 272, 1258
SduI GDGCHC 1 cut(s) 1266
SfaNI GCATC 7 cut(s) 102, 190, 624, 694, 705, 1452, 1488
SfcI CTRYAG 1 cut(s) 574
SmiMI CAYNNNNRTG 2 cut(s) 551, 1321
SphI GCATGC 1 cut(s) 556
Sse9I AATT 9 cut(s) 227, 300, 418, 725, 890, 942, 1032, 1204, 1491
SspMI CTAG 2 cut(s) 669, 737
StyD4I CCNGG 2 cut(s) 270, 1256
StyI CCWWGG 2 cut(s) 502, 1117
TaaI ACNGT 2 cut(s) 29, 1084
TaiI ACGT 1 cut(s) 1131
TaqI TCGA 2 cut(s) 158, 379
TasI AATT 9 cut(s) 227, 300, 418, 725, 890, 942, 1032, 1204, 1491
TfiI GAWTC 4 cut(s) 267, 310, 1294, 1333
Tru1I TTAA 2 cut(s) 777, 1178
Tru9I TTAA 2 cut(s) 777, 1178
TscAI CASTG 5 cut(s) 40, 361, 931, 1087, 1257
TseI GCWGC 2 cut(s) 438, 573
TspDTI ATGAA 9 cut(s) 39, 284, 320, 486, 993, 1104, 1116, 1326, 1484
TspGWI ACGGA 3 cut(s) 85, 440, 1196
TspRI CASTG 5 cut(s) 40, 361, 931, 1087, 1257
Van91I CCANNNNNTGG 4 cut(s) 96, 250, 1123, 1242
XapI RAATTY 3 cut(s) 418, 725, 1032
XceI RCATGY 1 cut(s) 556
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 669, 737
ZraI GACGTC 1 cut(s) 1129
Zsp2I ATGCAT 2 cut(s) 628, 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.