MD17G1090400.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000768
Physical Location & Seq
Forward (+)
7526215 .. 7528917
2703 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1090400.v1.1.491

Sequence Viewer

Length: 1485 bp
ATGCATAATAACACGCCTCTTCGATGCCCTGGCACCTACAAGCCAAGCATATGGAGTTACGATTTTGTTCAGTCCTTGAAGATTGATGACAACCCCCTGCAGGACAAAGTATACAAAGATGGGTGGAAGTTCCTAGAGGAGAAAGTGAAGCATATGATTGATTACAATGAAAATGATGATAATGATATCAACACGCTGACTACACTTGAGTTCATTGATGATATCCAACGGTTAGGTTTGGGATATCACTTTGAAAATGACATAAGAATAGCCCTTAATAAAATATTGAACAATAGTGATGGCCAAGGAACATACCAGAGTCTCCACCTCACCGCTCTCAGCTTCAGGCTCCTTAGACAACATGGGTTTCAGGTCTCCCAAGATGTATTTAGAAGATTCACGGACGATAATGGAAGTTTCAAGGCATGGCTTTGCAAGGATGTAAAAGGAATGTTGAGTCTGTATGAAGCTTCATATTTTGCTTTCGAAGGAGAGAGCCTCTTGGATGAGGGCCTTGCATTCTCAACAAATTACCTCAAGAACCTCAGTGGACTTAATGTTACCAATGGACTAGGAGAACAAGTGAGTCATGCACTGGAACTTCCACTGCACCATAGAATGCAAAGATTAGAATCTAGATGGTATATTGAGGCATACAGTAAAAGGCCAGATGCAAATAAGGTGCTACTTGAAATTGCCAAGCGAGATTTTAACAGGGTGCAATGCACACTCCAAAGGGATCTTCAAGAAGTATCAAGGTGGTGGGTGGATATGGGCTTAGCCGAAAAGTTGAGCTTCACCAGAGACAGATTGATGGAGTGCTTTTTTTGGTCGGTTGGAATCATCTTTGAACCTCAACACAGTCATGTCAGGAAAGAGCTGACAAAAGTGGCTGCTCTAGTAACCACCATTGATGATGTCTATGATGTTTATGGTACTCTGGATGAACTAGAGCTTTTTACTTCTGCTGTTGAAAGATGGGATATCAAAGCTGTAGAAATTCTTCCAGATTACATGAAGCTTTGTTTTCTAGCTCTTTATAACACAGTGAATAAGATGATTTATGAAACTCTAAAGAAGCAAGGAGAAAATGGGATCACAAAGCAAGCACTTGAATGCTTAGAGAATCACCATAGTCTCTTGCGTTGGCCATCTCTTATTTTCCGGCTTTCCAATGATTTGAGTACTTCGGCAGCAGAAGTGGAGAGAGGAGAAACTGCGAACTCAATCTCATGCATGATGCGTGGCAGTCTACATATTTCTGAGGAATCTGCTCGTCAATATATAAGTAATTTGGTTGAAAATAGTTGGAAGAAGCTGAACAAAGATGGAGCTTCTGCTACTCCATTTACTGAGCAGTTTGTAAAAGCAGCAAGAAACCTTGCTCGGATTTCCCAATGCATATACCAATATGGTGATGGGCATGGAGCTCCAGACACAAGAGCAAAGAATCGAATCCGATCAGTGATTATCGATCCCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko01110 map00900 map01110
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

495

Amino Acids

57.04

Weight (kDa)

5.83

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 17 - 199 1.3e-51 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 257 - 362 3.5e-45 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 296 - 365 4.1e-14 Terpene synthase family 2, C-terminal metal binding
Terpene_synth_C PF03936 365 - 439 3.4e-16 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 365 - 439 2.2e-13 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1041
AccB1I GGYRCC 1 cut(s) 32
AccBSI CCGCTC 1 cut(s) 335
AccI GTMKAC 2 cut(s) 111, 1252
AciI CCGC 1 cut(s) 333
AclWI GGATC 3 cut(s) 747, 1103, 1469
AcoI YGGCCR 2 cut(s) 301, 1148
AcsI RAATTY 1 cut(s) 999
AcuI CTGAAG 1 cut(s) 328
AfaI GTAC 2 cut(s) 937, 1186
AfiI CCNNNNNNNGG 1 cut(s) 100
AjnI CCWGG 1 cut(s) 28
AjuI GAANNNNNNNTTGG 2 cut(s) 726, 758
Alw21I GWGCWC 1 cut(s) 1432
Alw26I GTCTC 4 cut(s) 326, 379, 798, 1142
AlwI GGATC 3 cut(s) 747, 1103, 1469
AoxI GGCC 4 cut(s) 301, 511, 665, 1148
ApeKI GCWGC 3 cut(s) 893, 1193, 1370
ApoI RAATTY 1 cut(s) 999
Asp700I GAANNNNTTC 1 cut(s) 1002
AspS9I GGNCC 1 cut(s) 511
AsuHPI GGTGA 4 cut(s) 322, 790, 1121, 1427
AsuII TTCGAA 1 cut(s) 486
BalI TGGCCA 2 cut(s) 303, 1150
BanI GGYRCC 1 cut(s) 32
BanII GRGCYC 1 cut(s) 1432
BarI GAAGNNNNNNTAC 2 cut(s) 987, 1019
Bbv12I GWGCWC 1 cut(s) 1432
BbvI GCAGC 3 cut(s) 880, 1205, 1382
BccI CCATC 8 cut(s) 113, 293, 633, 808, 972, 1159, 1322, 1412
BciT130I CCWGG 1 cut(s) 30
BcoDI GTCTC 4 cut(s) 326, 379, 798, 1142
BfaI CTAG 6 cut(s) 134, 572, 636, 899, 950, 1031
BfmI CTRYAG 2 cut(s) 98, 993
BisI GCNGC 3 cut(s) 894, 1194, 1371
BlpI GCTNAGC 1 cut(s) 778
BlsI GCNGC 3 cut(s) 895, 1195, 1372
BmcAI AGTACT 1 cut(s) 1186
Bme1390I CCNGG 1 cut(s) 30
BmgT120I GGNCC 1 cut(s) 511
BmiI GGNNCC 2 cut(s) 34, 350
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 3 cut(s) 14, 661, 1230
BplI GAGNNNNNCTC 2 cut(s) 483, 515
BpmI CTGGAG 1 cut(s) 1416
Bpu1102I GCTNAGC 1 cut(s) 778
Bpu14I TTCGAA 1 cut(s) 486
BpuEI CTTGAG 2 cut(s) 227, 521
Bsa29I ATCGAT 1 cut(s) 1473
BsaBI GATNNNNATC 1 cut(s) 631
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 2 cut(s) 28, 304
BsaXI ACNNNNNCTCC 2 cut(s) 567, 597
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse1I ACTGG 1 cut(s) 600
Bse3DI GCAATG 1 cut(s) 728
Bse8I GATNNNNATC 1 cut(s) 631
BseBI CCWGG 1 cut(s) 30
BseCI ATCGAT 1 cut(s) 1473
BseDI CCNNGG 2 cut(s) 28, 304
BseGI GGATG 3 cut(s) 445, 511, 949
BseJI GATNNNNATC 1 cut(s) 631
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMI GCAATG 1 cut(s) 728
BseMII CTCAG 4 cut(s) 352, 559, 1254, 1344
BseNI ACTGG 1 cut(s) 600
BseRI GAGGAG 2 cut(s) 152, 1224
BseXI GCAGC 3 cut(s) 880, 1205, 1382
BsgI GTGCAG 1 cut(s) 593
BshFI GGCC 4 cut(s) 303, 513, 667, 1150
BshNI GGYRCC 1 cut(s) 32
BshVI ATCGAT 1 cut(s) 1473
BsiHKAI GWGCWC 1 cut(s) 1432
BsiSI CCGG 1 cut(s) 1165
BslI CCNNNNNNNGG 1 cut(s) 100
BsmAI GTCTC 4 cut(s) 326, 379, 798, 1142
BsmI GAATGC 3 cut(s) 518, 624, 1121
BsnI GGCC 4 cut(s) 303, 513, 667, 1150
Bso31I GGTCTC 1 cut(s) 379
Bsp119I TTCGAA 1 cut(s) 486
Bsp1286I GDGCHC 1 cut(s) 1432
Bsp143I GATC 4 cut(s) 739, 1095, 1460, 1474
Bsp1720I GCTNAGC 1 cut(s) 778
BspACI CCGC 1 cut(s) 333
BspANI GGCC 4 cut(s) 303, 513, 667, 1150
BspCNI CTCAG 4 cut(s) 351, 558, 1255, 1345
BspDI ATCGAT 1 cut(s) 1473
BspLI GGNNCC 2 cut(s) 34, 350
BspMAI CTGCAG 1 cut(s) 102
BspPI GGATC 3 cut(s) 747, 1103, 1469
BspT104I TTCGAA 1 cut(s) 486
BspT107I GGYRCC 1 cut(s) 32
BspTNI GGTCTC 1 cut(s) 379
BsrBI CCGCTC 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 728
BsrI ACTGG 1 cut(s) 600
BssECI CCNNGG 2 cut(s) 28, 304
BssMI GATC 4 cut(s) 739, 1095, 1460, 1474
BssNAI GTATAC 1 cut(s) 112
BssT1I CCWWGG 1 cut(s) 304
Bst1107I GTATAC 1 cut(s) 112
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 4 cut(s) 231, 659, 863, 1048
Bst6I CTCTTC 1 cut(s) 24
BstBI TTCGAA 1 cut(s) 486
BstC8I GCNNGC 1 cut(s) 1107
BstDEI CTNAG 7 cut(s) 338, 353, 545, 778, 1120, 1263, 1353
BstF5I GGATG 3 cut(s) 445, 511, 949
BstKTI GATC 4 cut(s) 742, 1098, 1463, 1477
BstMAI GTCTC 4 cut(s) 326, 379, 798, 1142
BstMBI GATC 4 cut(s) 739, 1095, 1460, 1474
BstNI CCWGG 1 cut(s) 30
BstSCI CCNGG 1 cut(s) 28
BstSFI CTRYAG 2 cut(s) 98, 993
BstV1I GCAGC 3 cut(s) 880, 1205, 1382
BstX2I RGATCY 1 cut(s) 739
BstXI CCANNNNNNTGG 1 cut(s) 51
BstYI RGATCY 1 cut(s) 739
BstZ17I GTATAC 1 cut(s) 112
Bsu15I ATCGAT 1 cut(s) 1473
BsuRI GGCC 4 cut(s) 303, 513, 667, 1150
BsuTUI ATCGAT 1 cut(s) 1473
BtsCI GGATG 3 cut(s) 445, 511, 949
BtsI GCAGTG 1 cut(s) 605
BtsIMutI CAGTG 5 cut(s) 553, 593, 605, 1053, 1470
Cac8I GCNNGC 1 cut(s) 1107
Cfr13I GGNCC 1 cut(s) 511
ClaI ATCGAT 1 cut(s) 1473
Csp6I GTAC 2 cut(s) 936, 1185
CviAII CATG 8 cut(s) 362, 426, 590, 866, 1015, 1233, 1237, 1424
CviQI GTAC 2 cut(s) 936, 1185
DdeI CTNAG 7 cut(s) 338, 353, 545, 778, 1120, 1263, 1353
DpnI GATC 4 cut(s) 741, 1097, 1462, 1476
DpnII GATC 4 cut(s) 739, 1095, 1460, 1474
EaeI YGGCCR 2 cut(s) 301, 1148
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
Ecl136II GAGCTC 1 cut(s) 1430
Eco130I CCWWGG 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 1432
Eco31I GGTCTC 1 cut(s) 379
Eco32I GATATC 4 cut(s) 187, 223, 245, 985
Eco53kI GAGCTC 1 cut(s) 1430
Eco57I CTGAAG 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 1430
EcoO109I RGGNCCY 1 cut(s) 511
EcoRII CCWGG 1 cut(s) 28
EcoRV GATATC 4 cut(s) 187, 223, 245, 985
EcoT14I CCWWGG 1 cut(s) 304
EcoT22I ATGCAT 3 cut(s) 6, 1238, 1403
EcoT38I GRGCYC 1 cut(s) 1432
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 8 cut(s) 365, 429, 593, 869, 1018, 1236, 1240, 1427
FalI AAGNNNNNCTT 2 cut(s) 779, 811
FatI CATG 8 cut(s) 361, 425, 589, 865, 1014, 1232, 1236, 1423
FauNDI CATATG 2 cut(s) 50, 153
FblI GTMKAC 2 cut(s) 111, 1252
Fnu4HI GCNGC 3 cut(s) 894, 1194, 1371
FokI GGATG 3 cut(s) 452, 518, 956
FriOI GRGCYC 1 cut(s) 1432
Fsp4HI GCNGC 3 cut(s) 894, 1194, 1371
FspBI CTAG 6 cut(s) 134, 572, 636, 899, 950, 1031
GluI GCNGC 3 cut(s) 894, 1194, 1371
GsuI CTGGAG 1 cut(s) 1416
HaeIII GGCC 4 cut(s) 303, 513, 667, 1150
HapII CCGG 1 cut(s) 1165
Hin1II CATG 8 cut(s) 365, 429, 593, 869, 1018, 1236, 1240, 1427
HindIII AAGCTT 2 cut(s) 468, 1019
HpaII CCGG 1 cut(s) 1165
HphI GGTGA 4 cut(s) 322, 790, 1121, 1427
Hpy166II GTNNAC 3 cut(s) 112, 551, 1253
Hpy188I TCNGA 3 cut(s) 1264, 1389, 1460
Hpy188III TCNNGA 7 cut(s) 538, 636, 746, 871, 941, 1007, 1433
Hpy8I GTNNAC 3 cut(s) 112, 551, 1253
HpyAV CCTTC 1 cut(s) 482
HpyCH4III ACNGT 4 cut(s) 231, 659, 863, 1048
HpyF3I CTNAG 7 cut(s) 338, 353, 545, 778, 1120, 1263, 1353
Hsp92II CATG 8 cut(s) 365, 429, 593, 869, 1018, 1236, 1240, 1427
Kzo9I GATC 4 cut(s) 739, 1095, 1460, 1474
LmnI GCTCC 4 cut(s) 354, 1331, 1427, 1435
Lsp1109I GCAGC 3 cut(s) 880, 1205, 1382
LweI GCATC 3 cut(s) 14, 661, 1230
MaeI CTAG 6 cut(s) 134, 572, 636, 899, 950, 1031
MaeIII GTNAC 3 cut(s) 56, 559, 901
MalI GATC 4 cut(s) 741, 1097, 1462, 1476
MbiI CCGCTC 1 cut(s) 335
MboI GATC 4 cut(s) 739, 1095, 1460, 1474
MboII GAAGA 6 cut(s) 11, 91, 405, 734, 995, 1324
MflI RGATCY 1 cut(s) 739
MhlI GDGCHC 1 cut(s) 1432
MlsI TGGCCA 2 cut(s) 303, 1150
MluCI AATT 4 cut(s) 529, 693, 999, 1291
MluNI TGGCCA 2 cut(s) 303, 1150
MlyI GAGTC 3 cut(s) 328, 466, 595
MmeI TCCRAC 3 cut(s) 250, 817, 1289
Mox20I TGGCCA 2 cut(s) 303, 1150
Mph1103I ATGCAT 3 cut(s) 6, 1238, 1403
MroXI GAANNNNTTC 1 cut(s) 1002
MscI TGGCCA 2 cut(s) 303, 1150
MseI TTAA 4 cut(s) 276, 555, 711, 1483
MslI CAYNNNNRTG 2 cut(s) 864, 1114
Msp20I TGGCCA 2 cut(s) 303, 1150
MspI CCGG 1 cut(s) 1165
MspR9I CCNGG 1 cut(s) 30
Mva1269I GAATGC 3 cut(s) 518, 624, 1121
MvaI CCWGG 1 cut(s) 30
NdeI CATATG 2 cut(s) 50, 153
NdeII GATC 4 cut(s) 739, 1095, 1460, 1474
NlaIII CATG 8 cut(s) 365, 429, 593, 869, 1018, 1236, 1240, 1427
NlaIV GGNNCC 2 cut(s) 34, 350
NsiI ATGCAT 3 cut(s) 6, 1238, 1403
NspV TTCGAA 1 cut(s) 486
PctI GAATGC 3 cut(s) 518, 624, 1121
PdmI GAANNNNTTC 1 cut(s) 1002
PfeI GAWTC 7 cut(s) 396, 632, 840, 1126, 1268, 1450, 1455
PkrI GCNGC 3 cut(s) 895, 1195, 1372
PleI GAGTC 3 cut(s) 327, 465, 594
PpsI GAGTC 3 cut(s) 327, 465, 594
PsiI TTATAA 1 cut(s) 1041
Psp124BI GAGCTC 1 cut(s) 1432
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 2 cut(s) 34, 350
PspPI GGNCC 1 cut(s) 511
PstI CTGCAG 1 cut(s) 102
PsuI RGATCY 1 cut(s) 739
RsaI GTAC 2 cut(s) 937, 1186
RsaNI GTAC 2 cut(s) 936, 1185
RseI CAYNNNNRTG 2 cut(s) 864, 1114
SacI GAGCTC 1 cut(s) 1432
SaqAI TTAA 4 cut(s) 276, 555, 711, 1483
SatI GCNGC 3 cut(s) 894, 1194, 1371
Sau3AI GATC 4 cut(s) 739, 1095, 1460, 1474
Sau96I GGNCC 1 cut(s) 511
SbfI CCTGCAGG 1 cut(s) 102
ScaI AGTACT 1 cut(s) 1186
SchI GAGTC 3 cut(s) 328, 466, 595
ScrFI CCNGG 1 cut(s) 30
SdaI CCTGCAGG 1 cut(s) 102
SduI GDGCHC 1 cut(s) 1432
SfaNI GCATC 3 cut(s) 14, 661, 1230
SfcI CTRYAG 2 cut(s) 98, 993
SfuI TTCGAA 1 cut(s) 486
SmiMI CAYNNNNRTG 2 cut(s) 864, 1114
SmlI CTYRAG 2 cut(s) 206, 536
SmoI CTYRAG 2 cut(s) 206, 536
Sse8387I CCTGCAGG 1 cut(s) 102
Sse9I AATT 4 cut(s) 529, 693, 999, 1291
SsiI CCGC 1 cut(s) 333
SspI AATATT 1 cut(s) 285
SspMI CTAG 6 cut(s) 134, 572, 636, 899, 950, 1031
SstI GAGCTC 1 cut(s) 1432
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 4 cut(s) 231, 659, 863, 1048
TaqI TCGA 4 cut(s) 22, 486, 1453, 1473
TasI AATT 4 cut(s) 529, 693, 999, 1291
TatI WGTACW 1 cut(s) 1184
TfiI GAWTC 7 cut(s) 396, 632, 840, 1126, 1268, 1450, 1455
Tru1I TTAA 4 cut(s) 276, 555, 711, 1483
Tru9I TTAA 4 cut(s) 276, 555, 711, 1483
TscAI CASTG 5 cut(s) 553, 600, 612, 1053, 1470
TseI GCWGC 3 cut(s) 893, 1193, 1370
TspDTI ATGAA 7 cut(s) 183, 202, 462, 480, 960, 1031, 1080
TspGWI ACGGA 1 cut(s) 416
TspRI CASTG 5 cut(s) 553, 600, 612, 1053, 1470
XapI RAATTY 1 cut(s) 999
XbaI TCTAGA 1 cut(s) 635
XcmI CCANNNNNNNNNTGG 1 cut(s) 1415
XmiI GTMKAC 2 cut(s) 111, 1252
XmnI GAANNNNTTC 1 cut(s) 1002
XspI CTAG 6 cut(s) 134, 572, 636, 899, 950, 1031
ZrmI AGTACT 1 cut(s) 1186
Zsp2I ATGCAT 3 cut(s) 6, 1238, 1403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.