FvH4_6g45600

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000768
Physical Location & Seq
Forward (+)
34972708 .. 34975306
2599 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45600.t1

Sequence Viewer

Length: 1728 bp
ATGTCTACTCAAACTTCGGGTGTTAAGAGAGTGACACCAGATTACAAGCCAAGCATTTGGAATGATGAGTTTCTTCATTCGTTACAGATTCATGATTGTAAACAGGTGTACATTAGTGTTGACTACTTTATGCTTGATTTTGCAGATCAACCGTATGAAGATAATAAGTGGAAGTCGACGGAGGAGAGTGTGAAACATATGATTGATGATGCGCAGTCCGTAGAGACTACACTTGAATTCATTGATGATATCCAACGATTAGGTTTGGGACACCGATTAGAGACCAACATAACACGAGTCCTTGAAAGAATTATGAAGAAGCTTGAACAAAAAACAGATGAGGATACTCCAAGTCTCCACCTCACTGCTCTCAGATTCAGGCTCCTTCGACAGCATGGGTTTCAACTCTCCCAAGATGTCTTCAAGATATTCACAGACTGCGACGGCAGCTTCAATGATCGCCTTTGCAAAGATGTCAAAGGAATGCTGAGTCTGTATGAAGCTTCATTCCTTGGTTTTGAAGGAGAAACCCTCTTGGATGAGGCCCTTGCATTCACCAGCATGCACCTAAAGAACCTCAGCTGCAGGTTACTTGATACTCACGGCAGAAATGTATTAGAGCAAGTGAGTCATGCATTGGAGATGCCATTGCACCACAGAATGCAAAGATTAGAAGCTAGATGGTACATTGAGGCATACAATAAAAGGGCAGATGCAAATCAGGTGCTACTTGAATTTGCCAAGCTAGATTACAATGCGGTGCAACAAACATACCAAAGAGATCTTAAGGACTTGTCAAGGTGGTGGAAGGATATGGGATTAGCAAAGAAGTTGCCCTTCGCCAAAGACAGATTGATGGAGTGCTTCTTTTGGTCAGTTGGTATAGTGTTTGAACCTCAACTCAGCAATCTCAGAAAAGGGATAACTAAAGTCAGTGCTCTTATAAGCACTGTTGATGATGTCTATGATGTTTATGCCACTTACGATGAACTAGAGCAATTCACATCTCTTGTTGAAAGATGGGATGTGAATGCTGCAGTTGAAACTCTCCCAGACTACAAGCTGAAGCTATGTTTCCTTGCCCTCTATAACACTGTGAATGAAATGGCTTATGAAACTTTGAAGGAGCAAGGAGTGGACGTCCTTCCTTACTTGAGAAAAGCTTGGGCTGATTTGTGCAAAGCTTTCTTAAGGGAGGCAGAGTGGAGTAACAACAAGTACACACCCACATTTGAAGAGTATCTTGCCAATGCATGGATATCAGCGTCCGCGGTGGTCATTTTAGTTCATGCCTACTTCCTACTGAATCAAAATATCTCAGCTGAAGCACTTGGATGCTTAGAGAATCCCCACAATCTCTTGCGTTGGCCGTCTCTTATTTTCCGGCTAACAAACGATTTGGCTACTTCCGAGGCAGAACTAGAGAGAGGTGAAACTGCAAATTCAATATCCTGCCTCATACGTGGAAGTAGCAATGAAGTCTGCGATGAGGAATCTGCTCGCAAATATATAAGTAATTTGATTGAGAATAGTTGGAAGAAGATGAACAAAGATGGACAACTTTTTGGTGCTAGTAGTGCTTCTTCTCCATTTACAAAGGAATTCGTGGCAGCAGGGACAAACCTCTCTCGGATTGCCCAATGCATATACCAATATGGAGATGGGATTGGTGCCCCTGACAAAATAGTAAAGAACAGGATTCTAGCAGTGATTATGCAACCCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko01110 map00900 map01110
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

576

Amino Acids

66.15

Weight (kDa)

5.31

Isoelectric Point (pI)

35.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 20 - 213 2.5e-47 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 270 - 514 1e-86 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 310 - 514 8.1e-56 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 944
AatII GACGTC 1 cut(s) 1143
Acc16I TGCGCA 1 cut(s) 213
Acc36I ACCTGC 1 cut(s) 576
AccB1I GGYRCC 1 cut(s) 1670
AccB7I CCANNNNNTGG 1 cut(s) 1254
AccI GTMKAC 2 cut(s) 5, 176
AccII CGCG 1 cut(s) 1271
AciI CCGC 3 cut(s) 758, 1269, 1271
AcoI YGGCCR 1 cut(s) 1367
AcsI RAATTY 4 cut(s) 236, 734, 1441, 1601
AcuI CTGAAG 2 cut(s) 1085, 1344
AcyI GRCGYC 1 cut(s) 1140
AfaI GTAC 3 cut(s) 110, 686, 1220
AfiI CCNNNNNNNGG 1 cut(s) 1254
AflII CTTAAG 2 cut(s) 785, 1189
Alw21I GWGCWC 1 cut(s) 940
Alw26I GTCTC 4 cut(s) 218, 275, 359, 1377
AoxI GGCC 2 cut(s) 543, 1367
ApeKI GCWGC 4 cut(s) 447, 582, 1034, 1610
ApoI RAATTY 4 cut(s) 236, 734, 1441, 1601
AspLEI GCGC 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 544
AsuHPI GGTGA 2 cut(s) 547, 1442
BaeGI GKGCMC 1 cut(s) 1675
BanI GGYRCC 1 cut(s) 1670
BarI GAAGNNNNNNTAC 2 cut(s) 1567, 1599
BauI CACGAG 1 cut(s) 294
BbsI GAAGAC 1 cut(s) 412
Bbv12I GWGCWC 1 cut(s) 940
BbvCI CCTCAGC 1 cut(s) 578
BbvI GCAGC 4 cut(s) 459, 569, 1021, 1622
BccI CCATC 5 cut(s) 675, 850, 1014, 1547, 1655
BceAI ACGGC 3 cut(s) 460, 619, 1354
BciVI GTATCC 1 cut(s) 337
BcoDI GTCTC 4 cut(s) 218, 275, 359, 1377
BfaI CTAG 6 cut(s) 678, 746, 992, 1421, 1572, 1703
BfmI CTRYAG 2 cut(s) 583, 1035
BfrI CTTAAG 2 cut(s) 785, 1189
BfuAI ACCTGC 1 cut(s) 576
BfuI GTATCC 1 cut(s) 337
BglII AGATCT 1 cut(s) 781
BisI GCNGC 4 cut(s) 448, 583, 1035, 1611
BlsI GCNGC 4 cut(s) 449, 584, 1036, 1612
BmgT120I GGNCC 1 cut(s) 544
BmiI GGNNCC 2 cut(s) 383, 1672
BmsI GCATC 4 cut(s) 199, 633, 703, 1325
BpiI GAAGAC 1 cut(s) 412
BplI GAGNNNNNCTC 2 cut(s) 516, 548
Bpu10I CCTNAGC 1 cut(s) 578
BpuEI CTTGAG 1 cut(s) 1174
BsaAI YACGTR 1 cut(s) 1463
BsaBI GATNNNNATC 1 cut(s) 717
BsaHI GRCGYC 1 cut(s) 1140
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 3 cut(s) 511, 1269, 1410
BsaXI ACNNNNNCTCC 4 cut(s) 1125, 1155, 1187, 1217
Bsc4I CCNNNNNNNGG 1 cut(s) 1254
Bse3DI GCAATG 2 cut(s) 647, 1480
Bse8I GATNNNNATC 1 cut(s) 717
BseDI CCNNGG 3 cut(s) 511, 1269, 1410
BseGI GGATG 3 cut(s) 544, 1030, 1340
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 1 cut(s) 1254
BseMI GCAATG 2 cut(s) 647, 1480
BseMII CTCAG 6 cut(s) 385, 479, 592, 916, 925, 1332
BseRI GAGGAG 1 cut(s) 197
BseSI GKGCMC 1 cut(s) 1675
BseXI GCAGC 4 cut(s) 459, 569, 1021, 1622
Bsh1236I CGCG 1 cut(s) 1271
BshFI GGCC 2 cut(s) 545, 1369
BshNI GGYRCC 1 cut(s) 1670
BsiHKAI GWGCWC 1 cut(s) 940
BsiSI CCGG 1 cut(s) 1384
BslFI GGGAC 2 cut(s) 282, 1630
BslI CCNNNNNNNGG 1 cut(s) 1254
BsmAI GTCTC 4 cut(s) 218, 275, 359, 1377
BsmBI CGTCTC 1 cut(s) 1377
BsmFI GGGAC 2 cut(s) 282, 1630
BsmI GAATGC 4 cut(s) 489, 551, 666, 1036
BsnI GGCC 2 cut(s) 545, 1369
Bso31I GGTCTC 1 cut(s) 275
Bsp1286I GDGCHC 2 cut(s) 940, 1675
Bsp1407I TGTACA 1 cut(s) 108
Bsp143I GATC 3 cut(s) 145, 457, 781
BspACI CCGC 3 cut(s) 758, 1269, 1271
BspANI GGCC 2 cut(s) 545, 1369
BspCNI CTCAG 6 cut(s) 384, 480, 591, 915, 924, 1331
BspFNI CGCG 1 cut(s) 1271
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 2 cut(s) 383, 1672
BspMAI CTGCAG 2 cut(s) 587, 1039
BspMI ACCTGC 1 cut(s) 576
BspT107I GGYRCC 1 cut(s) 1670
BspTI CTTAAG 2 cut(s) 785, 1189
BspTNI GGTCTC 1 cut(s) 275
BsrDI GCAATG 2 cut(s) 647, 1480
BsrGI TGTACA 1 cut(s) 108
BssECI CCNNGG 3 cut(s) 511, 1269, 1410
BssMI GATC 3 cut(s) 145, 457, 781
BssNI GRCGYC 1 cut(s) 1140
BssSI CACGAG 1 cut(s) 294
BssT1I CCWWGG 1 cut(s) 511
Bst2BI CACGAG 1 cut(s) 294
Bst4CI ACNGT 3 cut(s) 153, 952, 1096
Bst6I CTCTTC 1 cut(s) 1230
BstACI GRCGYC 1 cut(s) 1140
BstAFI CTTAAG 2 cut(s) 785, 1189
BstAUI TGTACA 1 cut(s) 108
BstBAI YACGTR 1 cut(s) 1463
BstC8I GCNNGC 2 cut(s) 563, 1501
BstDEI CTNAG 7 cut(s) 371, 488, 578, 902, 911, 1318, 1339
BstDSI CCRYGG 1 cut(s) 1269
BstF5I GGATG 3 cut(s) 544, 1030, 1340
BstFNI CGCG 1 cut(s) 1271
BstHHI GCGC 1 cut(s) 214
BstKTI GATC 3 cut(s) 148, 460, 784
BstMAI GTCTC 4 cut(s) 218, 275, 359, 1377
BstMBI GATC 3 cut(s) 145, 457, 781
BstMWI GCNNNNNNNGC 2 cut(s) 447, 1577
BstNSI RCATGY 1 cut(s) 565
BstSFI CTRYAG 2 cut(s) 583, 1035
BstSLI GKGCMC 1 cut(s) 1675
BstUI CGCG 1 cut(s) 1271
BstV1I GCAGC 4 cut(s) 459, 569, 1021, 1622
BstV2I GAAGAC 1 cut(s) 412
BstX2I RGATCY 1 cut(s) 781
BstXI CCANNNNNNTGG 1 cut(s) 57
BstYI RGATCY 1 cut(s) 781
BsuI GTATCC 1 cut(s) 337
BsuRI GGCC 2 cut(s) 545, 1369
BtgI CCRYGG 1 cut(s) 1269
BtgZI GCGATG 1 cut(s) 1500
BtsCI GGATG 3 cut(s) 544, 1030, 1340
BtsI GCAGTG 2 cut(s) 363, 1713
BtsIMutI CAGTG 5 cut(s) 363, 940, 948, 1092, 1713
BveI ACCTGC 1 cut(s) 576
Cac8I GCNNGC 2 cut(s) 563, 1501
CciI TCATGA 1 cut(s) 91
CfoI GCGC 1 cut(s) 214
Cfr13I GGNCC 1 cut(s) 544
Cfr42I CCGCGG 1 cut(s) 1272
CseI GACGC 1 cut(s) 1254
Csp6I GTAC 3 cut(s) 109, 685, 1219
CviAII CATG 6 cut(s) 92, 395, 562, 632, 1254, 1289
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 7 cut(s) 371, 488, 578, 902, 911, 1318, 1339
DpnI GATC 3 cut(s) 147, 459, 783
DpnII GATC 3 cut(s) 145, 457, 781
EaeI YGGCCR 1 cut(s) 1367
Eam1104I CTCTTC 1 cut(s) 1230
EarI CTCTTC 1 cut(s) 1230
Eco130I CCWWGG 1 cut(s) 511
Eco31I GGTCTC 1 cut(s) 275
Eco32I GATATC 2 cut(s) 250, 1260
Eco57I CTGAAG 2 cut(s) 1085, 1344
EcoO109I RGGNCCY 1 cut(s) 544
EcoRI GAATTC 2 cut(s) 236, 1601
EcoRV GATATC 2 cut(s) 250, 1260
EcoT14I CCWWGG 1 cut(s) 511
EcoT22I ATGCAT 3 cut(s) 637, 1255, 1646
ErhI CCWWGG 1 cut(s) 511
Esp3I CGTCTC 1 cut(s) 1377
FaeI CATG 6 cut(s) 95, 398, 565, 635, 1257, 1292
FalI AAGNNNNNCTT 4 cut(s) 821, 853, 1227, 1259
FaqI GGGAC 2 cut(s) 282, 1630
FatI CATG 6 cut(s) 91, 394, 561, 631, 1253, 1288
FauNDI CATATG 1 cut(s) 198
FblI GTMKAC 2 cut(s) 5, 176
Fnu4HI GCNGC 4 cut(s) 448, 583, 1035, 1611
FokI GGATG 3 cut(s) 551, 1037, 1347
Fsp4HI GCNGC 4 cut(s) 448, 583, 1035, 1611
FspBI CTAG 6 cut(s) 678, 746, 992, 1421, 1572, 1703
FspI TGCGCA 1 cut(s) 213
GlaI GCGC 1 cut(s) 213
GluI GCNGC 4 cut(s) 448, 583, 1035, 1611
HaeIII GGCC 2 cut(s) 545, 1369
HapII CCGG 1 cut(s) 1384
HgaI GACGC 1 cut(s) 1254
HhaI GCGC 1 cut(s) 214
Hin1I GRCGYC 1 cut(s) 1140
Hin1II CATG 6 cut(s) 95, 398, 565, 635, 1257, 1292
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HincII GTYRAC 2 cut(s) 121, 177
HindII GTYRAC 2 cut(s) 121, 177
HindIII AAGCTT 4 cut(s) 320, 501, 1161, 1182
HinfI GANTC 9 cut(s) 88, 297, 375, 490, 628, 1306, 1345, 1493, 1699
HpaII CCGG 1 cut(s) 1384
HphI GGTGA 2 cut(s) 547, 1442
Hpy166II GTNNAC 7 cut(s) 6, 101, 109, 121, 177, 1138, 1221
Hpy188I TCNGA 4 cut(s) 374, 914, 1411, 1632
Hpy188III TCNNGA 2 cut(s) 92, 424
Hpy8I GTNNAC 7 cut(s) 6, 101, 109, 121, 177, 1138, 1221
Hpy99I CGWCG 2 cut(s) 181, 446
HpyAV CCTTC 6 cut(s) 395, 515, 802, 847, 1117, 1154
HpyCH4III ACNGT 3 cut(s) 153, 952, 1096
HpyCH4IV ACGT 2 cut(s) 1140, 1462
HpyF10VI GCNNNNNNNGC 2 cut(s) 447, 1577
HpyF3I CTNAG 7 cut(s) 371, 488, 578, 902, 911, 1318, 1339
HpySE526I ACGT 2 cut(s) 1140, 1462
Hsp92I GRCGYC 1 cut(s) 1140
Hsp92II CATG 6 cut(s) 95, 398, 565, 635, 1257, 1292
HspAI GCGC 1 cut(s) 212
KspI CCGCGG 1 cut(s) 1272
Kzo9I GATC 3 cut(s) 145, 457, 781
LmnI GCTCC 2 cut(s) 387, 1126
Lsp1109I GCAGC 4 cut(s) 459, 569, 1021, 1622
LweI GCATC 4 cut(s) 199, 633, 703, 1325
MaeI CTAG 6 cut(s) 678, 746, 992, 1421, 1572, 1703
MaeII ACGT 2 cut(s) 1140, 1462
MaeIII GTNAC 4 cut(s) 31, 81, 588, 1208
MalI GATC 3 cut(s) 147, 459, 783
MboI GATC 3 cut(s) 145, 457, 781
MboII GAAGA 8 cut(s) 65, 170, 328, 412, 1247, 1549, 1552, 1575
MflI RGATCY 1 cut(s) 781
MhlI GDGCHC 2 cut(s) 940, 1675
MluCI AATT 7 cut(s) 236, 309, 734, 998, 1441, 1516, 1601
MlyI GAGTC 3 cut(s) 306, 499, 637
MmeI TCCRAC 2 cut(s) 277, 1514
Mph1103I ATGCAT 3 cut(s) 637, 1255, 1646
MseI TTAA 4 cut(s) 24, 786, 1190, 1726
MslI CAYNNNNRTG 2 cut(s) 560, 1333
MspA1I CMGCKG 3 cut(s) 582, 1271, 1322
MspCI CTTAAG 2 cut(s) 785, 1189
MspI CCGG 1 cut(s) 1384
Mva1269I GAATGC 4 cut(s) 489, 551, 666, 1036
MvnI CGCG 1 cut(s) 1271
MwoI GCNNNNNNNGC 2 cut(s) 447, 1577
NdeI CATATG 1 cut(s) 198
NdeII GATC 3 cut(s) 145, 457, 781
NlaIII CATG 6 cut(s) 95, 398, 565, 635, 1257, 1292
NlaIV GGNNCC 2 cut(s) 383, 1672
NmuCI GTSAC 1 cut(s) 31
NsbI TGCGCA 1 cut(s) 213
NsiI ATGCAT 3 cut(s) 637, 1255, 1646
NspI RCATGY 1 cut(s) 565
PaeI GCATGC 1 cut(s) 565
PagI TCATGA 1 cut(s) 91
PctI GAATGC 4 cut(s) 489, 551, 666, 1036
PfeI GAWTC 6 cut(s) 88, 375, 1306, 1345, 1493, 1699
PflMI CCANNNNNTGG 1 cut(s) 1254
PkrI GCNGC 4 cut(s) 449, 584, 1036, 1612
PleI GAGTC 3 cut(s) 305, 498, 636
PpsI GAGTC 3 cut(s) 305, 498, 636
Ppu21I YACGTR 1 cut(s) 1463
PsiI TTATAA 1 cut(s) 944
PspN4I GGNNCC 2 cut(s) 383, 1672
PspPI GGNCC 1 cut(s) 544
PstI CTGCAG 2 cut(s) 587, 1039
PsuI RGATCY 1 cut(s) 781
PvuII CAGCTG 2 cut(s) 582, 1322
RsaI GTAC 3 cut(s) 110, 686, 1220
RsaNI GTAC 3 cut(s) 109, 685, 1219
RseI CAYNNNNRTG 2 cut(s) 560, 1333
SacII CCGCGG 1 cut(s) 1272
SalI GTCGAC 1 cut(s) 175
SaqAI TTAA 4 cut(s) 24, 786, 1190, 1726
SatI GCNGC 4 cut(s) 448, 583, 1035, 1611
Sau3AI GATC 3 cut(s) 145, 457, 781
Sau96I GGNCC 1 cut(s) 544
SchI GAGTC 3 cut(s) 306, 499, 637
SduI GDGCHC 2 cut(s) 940, 1675
SfaNI GCATC 4 cut(s) 199, 633, 703, 1325
SfcI CTRYAG 2 cut(s) 583, 1035
Sfr303I CCGCGG 1 cut(s) 1272
SgrBI CCGCGG 1 cut(s) 1272
SmiMI CAYNNNNRTG 2 cut(s) 560, 1333
SmlI CTYRAG 3 cut(s) 785, 1153, 1189
SmoI CTYRAG 3 cut(s) 785, 1153, 1189
SphI GCATGC 1 cut(s) 565
Sse9I AATT 7 cut(s) 236, 309, 734, 998, 1441, 1516, 1601
SsiI CCGC 3 cut(s) 758, 1269, 1271
SspMI CTAG 6 cut(s) 678, 746, 992, 1421, 1572, 1703
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 3 cut(s) 153, 952, 1096
TaiI ACGT 2 cut(s) 1143, 1465
TaqI TCGA 2 cut(s) 176, 388
TasI AATT 7 cut(s) 236, 309, 734, 998, 1441, 1516, 1601
TatI WGTACW 2 cut(s) 108, 1218
TfiI GAWTC 6 cut(s) 88, 375, 1306, 1345, 1493, 1699
Tru1I TTAA 4 cut(s) 24, 786, 1190, 1726
Tru9I TTAA 4 cut(s) 24, 786, 1190, 1726
TscAI CASTG 5 cut(s) 370, 940, 955, 1099, 1713
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 4 cut(s) 447, 582, 1034, 1610
Tsp45I GTSAC 1 cut(s) 31
TspGWI ACGGA 2 cut(s) 194, 208
TspRI CASTG 5 cut(s) 370, 940, 955, 1099, 1713
Van91I CCANNNNNTGG 1 cut(s) 1254
Vha464I CTTAAG 2 cut(s) 785, 1189
XapI RAATTY 4 cut(s) 236, 734, 1441, 1601
XceI RCATGY 1 cut(s) 565
XcmI CCANNNNNNNNNTGG 1 cut(s) 1658
XmiI GTMKAC 2 cut(s) 5, 176
XspI CTAG 6 cut(s) 678, 746, 992, 1421, 1572, 1703
ZraI GACGTC 1 cut(s) 1141
Zsp2I ATGCAT 3 cut(s) 637, 1255, 1646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.