FvH4_7g12250

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000330
Physical Location & Seq
Reverse (-)
11097689 .. 11099528
1840 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g12250.t1

Sequence Viewer

Length: 1536 bp
ATGGCTTTTCTGTATTTCATCTTCACCTTCACATGTTCATTACTCTTTCTTTTACTCTTCCTCTTCTCTTTTCTCATGCTCAGAATCTTCGCAGGCAAGTCCATCAGACACCCAAACTACCCACCTGTAAAAGGCACCACCTTTGATCACCTGTTATACTACAACAAACTATATGACTACCACACCCGAGTTGCGAGAGAAGGCCCAACTCACCGGCTTCTTTCTCTAAACAGCAGCATGATATACACTTGTGACCCTCGAAACATTGAGCATTTTCTCAAGACCAACTTTGCTCATTACTCAAAAAGTGACTATAACAAAACTATTATGTCTGAGATTTTGGGGCAGGGGATTTTTGTTGTTAATGGAGAGAAATGGAAGCAGCAGAGGAAGCTTGCAAGCTATGAATTTTCGACAAGGAATCTTAGAGATTTCAGCTGTTCTGTATTTAGAGGAAATGCTGCCAAACTCGTTAAATTTCTTTCAGAGTTTTCGAATTCCAAAAGGGTGTTTGATATGCAGGAGTTGCTTATGAGATGTACCTTGGATTCCATATTCAAAGTTGGATTTGGAACAGAACTGAATTGCTTGGAGGGGTTAAGCAAAGATGGGATTGCATTTATGAAGGCCTTTGATGAATCAGCTGCTCTAACTTACTGGCGTTACGTTGATCCATTCTGGAAGTTGAAAAGATTTCTCAATATTGGTTCTGAGGCCACTCTTAAACAGTATGTCAAAGTCACCCATGATTTTGTGCACCAACTTATAAGGAACAAGAGGGAACTTCTAGCTGCTAAGAGAGATGGTATAAGTGACAAGGAGGACATACTATCAAGGTTTCTAATGGAGAGCGAAAAGGATCCAGTGGGAATGAATGATGACTATCTAAGTGATATAATTCTGAATTTTATGATTGCTGGAAAAGATACAAGTGCCAATACACTATCATGGTTCTTCTACATGCTCAGCAAGAACCCTCTAATACAGGGGAAAGTTGCACAAGAAGTGAGGGATGTTGTCGGAATGAATCACGACTCTGACATTGATGAATTTGTGAGGAACATAACTGATGCAAATCTTGAAAAGATGCATTACCTTCACGCGACACTAACAGAAACCTTGAGGCTCTACCCTGCAGTTCCTGTGGATGGGAGGTATGCAGAGAAAGATGACATTCTTCCTGATGGCTTTAGAGTGAAAAAAGGAGACAGAATAGCTTACATGGCCTATGCCATGGGGAGAATGCCTTACGTTTGGGGAGAAGATGCTGAGGTTTTCCGACCTGAAAGATGGCTCGACAATGGAGTTTTCAAGCCCGAATCACCTTTCAAATTCATCGCATTTCATGCAGGGCCTCGGACCTGTCTAGGGAGGGACTTTGCTTACAGGCAGATGAAGATAGTAGCAATGGCTCTGCTTTGCTTCTTCCGGTTCAAATTGGCTGATGAAACAAGAAATGTAACATATAGGACCATGTTCACCCTTCACATTGATGGCACCCTCCCTATGCTTGCACTTCCAAGAAAAACCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

512

Amino Acids

59.49

Weight (kDa)

9.01

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 54 - 495 7e-79 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44890 AT2G44890 AT2G45510
fragaria_vesca FvH4_7g12200 FvH4_7g12250 FvH4_7g12281 FvH4_7g12281 FvH4_7g12281 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12300 FvH4_7g12310
malus_domestica MD02G1200000.v1.1 MD02G1200100.v1.1 MD02G1200300.v1.1 MD02G1200400.v1.1 MD02G1200500.v1.1 MD02G1200600.v1.1 MD02G1200700.v1.1
prunus_persica Prupe.2G148400_v2.0.a1 Prupe.2G148500_v2.0.a1 Prupe.2G148600_v2.0.a1 Prupe.2G148700_v2.0.a1
pyrus_communis pycom02g16390
rosa_chinensis RchiOBHm_Chr1g0350941 RchiOBHm_Chr1g0350951 RchiOBHm_Chr1g0350981 RchiOBHm_Chr1g0350991 RchiOBHm_Chr1g0351001 RchiOBHm_Chr1g0351031 RchiOBHm_Chr1g0351061 RchiOBHm_Chr1g0351071 RchiOBHm_Chr1g0351081 RchiOBHm_Chr1g0351091 RchiOBHm_Chr1g0351101 RchiOBHm_Chr1g0375431 RchiOBHm_Chr2g0142281
rosa_laevigata RLG00000026651 RLG00000028432 RLG00000028433 RLG00000028435 RLG00000028436 RLG00000028437 RLG00000028438 RLG00000028439
rosa_multiflora Rmu_co7992102.1_g000001 Rmu_sc0000429.1_g000085 Rmu_sc0002665.1_g000014 Rmu_sc0002665.1_g000018 Rmu_sc0006087.1_g000001 Rmu_sc0006458.1_g000011 Rmu_sc0008688.1_g000002 Rmu_sc0008688.1_g000006 Rmu_sc0009646.1_g000012 Rmu_sc0032693.1_g000003
rosa_roxburghii Rroxscaffold_4G00304080 Rroxscaffold_4G00304120 Rroxscaffold_4G00304150 Rroxscaffold_4G00304170 Rroxscaffold_4G00304180 Rroxscaffold_4G00304190 Rroxscaffold_4G00304210 Rroxscaffold_4G00304230 Rroxscaffold_4G00304280
rosa_rugosa Rorug01G0075700 Rorug01G0215400 Rorug01G0216000 Rorug01G0216100 Rorug01G0216200 Rorug01G0216300 Rorug01G0216300 Rorug01G0216300 Rorug02G0238800 Rorug04G0006700 Rorug06G0023400
rosa_samantha Rh1AG230300 Rh1AG230400 Rh1AG230700 Rh1AG230800 Rh1BG200100 Rh1BG200200 Rh1BG200600 Rh1DG227900 Rh1DG228000 Rh1DG228200
rosa_wichuraiana Rw0G010880 Rw1G019960 Rw1G019970 Rw1G019990 Rw1G020000 Rw1G020010 Rw1G020040 Rw1G020050 Rw1G020060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 767
AccB1I GGYRCC 2 cut(s) 134, 1496
AccII CGCG 1 cut(s) 1103
AclWI GGATC 3 cut(s) 665, 854, 867
AcsI RAATTY 6 cut(s) 407, 476, 496, 904, 1049, 1331
AfaI GTAC 1 cut(s) 541
AfiI CCNNNNNNNGG 3 cut(s) 131, 1148, 1368
AflIII ACRYGT 1 cut(s) 32
AgsI TTSAA 6 cut(s) 559, 688, 1082, 1312, 1330, 1435
AluBI AGCT 6 cut(s) 394, 402, 438, 644, 791, 1217
AluI AGCT 6 cut(s) 394, 402, 438, 644, 791, 1217
Alw21I GWGCWC 1 cut(s) 759
Alw26I GTCTC 1 cut(s) 1200
Alw44I GTGCAC 1 cut(s) 755
AlwI GGATC 3 cut(s) 665, 854, 867
AlwNI CAGNNNCTG 1 cut(s) 1142
Ama87I CYCGRG 1 cut(s) 186
AoxI GGCC 5 cut(s) 202, 627, 714, 1224, 1352
ApaLI GTGCAC 1 cut(s) 755
ApeKI GCWGC 5 cut(s) 234, 382, 461, 644, 791
ApoI RAATTY 6 cut(s) 407, 476, 496, 904, 1049, 1331
AspS9I GGNCC 4 cut(s) 203, 1352, 1359, 1470
AsuHPI GGTGA 6 cut(s) 16, 140, 203, 733, 1314, 1471
AsuII TTCGAA 1 cut(s) 494
AvaI CYCGRG 1 cut(s) 186
AvaII GGWCC 2 cut(s) 1359, 1470
BaeGI GKGCMC 1 cut(s) 759
BamHI GGATCC 1 cut(s) 859
BanI GGYRCC 2 cut(s) 134, 1496
BarI GAAGNNNNNNTAC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 759
BbvCI CCTCAGC 1 cut(s) 1269
BbvI GCAGC 5 cut(s) 246, 394, 448, 631, 778
BccI CCATC 7 cut(s) 110, 602, 797, 1142, 1178, 1284, 1487
BclI TGATCA 1 cut(s) 145
BcoDI GTCTC 1 cut(s) 1200
BfaI CTAG 2 cut(s) 788, 1367
BfmI CTRYAG 1 cut(s) 1134
BisI GCNGC 5 cut(s) 235, 383, 462, 645, 792
BlpI GCTNAGC 1 cut(s) 965
BlsI GCNGC 5 cut(s) 236, 384, 463, 646, 793
Bme18I GGWCC 2 cut(s) 1359, 1470
BmeT110I CYCGRG 1 cut(s) 186
BmgT120I GGNCC 4 cut(s) 203, 1352, 1359, 1470
BmiI GGNNCC 3 cut(s) 136, 861, 1498
BmsI GCATC 3 cut(s) 1060, 1077, 1255
Bpu10I CCTNAGC 1 cut(s) 1269
Bpu1102I GCTNAGC 1 cut(s) 965
Bpu14I TTCGAA 1 cut(s) 494
BpuEI CTTGAG 2 cut(s) 263, 1141
BsaBI GATNNNNATC 2 cut(s) 882, 1074
BsaJI CCNNGG 3 cut(s) 543, 1233, 1355
BsaWI WCCGGW 1 cut(s) 1428
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 1148, 1368
Bse118I RCCGGY 1 cut(s) 213
Bse1I ACTGG 2 cut(s) 662, 863
Bse3DI GCAATG 1 cut(s) 1413
Bse8I GATNNNNATC 2 cut(s) 882, 1074
BseDI CCNNGG 3 cut(s) 543, 1233, 1355
BseGI GGATG 2 cut(s) 1018, 1153
BseJI GATNNNNATC 2 cut(s) 882, 1074
BseLI CCNNNNNNNGG 3 cut(s) 131, 1148, 1368
BseMI GCAATG 1 cut(s) 1413
BseMII CTCAG 5 cut(s) 94, 324, 702, 979, 1260
BseNI ACTGG 2 cut(s) 662, 863
BseSI GKGCMC 1 cut(s) 759
BseXI GCAGC 5 cut(s) 246, 394, 448, 631, 778
Bsh1236I CGCG 1 cut(s) 1103
BshFI GGCC 5 cut(s) 204, 629, 716, 1226, 1354
BshNI GGYRCC 2 cut(s) 134, 1496
BsiHKAI GWGCWC 1 cut(s) 759
BsiHKCI CYCGRG 1 cut(s) 186
BsiSI CCGG 2 cut(s) 214, 1429
BslFI GGGAC 1 cut(s) 1388
BslI CCNNNNNNNGG 3 cut(s) 131, 1148, 1368
BsmAI GTCTC 1 cut(s) 1200
BsmFI GGGAC 1 cut(s) 1388
BsmI GAATGC 1 cut(s) 1248
BsnI GGCC 5 cut(s) 204, 629, 716, 1226, 1354
BsoBI CYCGRG 1 cut(s) 186
Bsp119I TTCGAA 1 cut(s) 494
Bsp1286I GDGCHC 1 cut(s) 759
Bsp143I GATC 3 cut(s) 145, 670, 859
Bsp1720I GCTNAGC 1 cut(s) 965
Bsp19I CCATGG 1 cut(s) 1233
BspANI GGCC 5 cut(s) 204, 629, 716, 1226, 1354
BspCNI CTCAG 5 cut(s) 93, 325, 703, 978, 1261
BspFNI CGCG 1 cut(s) 1103
BspLI GGNNCC 3 cut(s) 136, 861, 1498
BspMAI CTGCAG 1 cut(s) 1138
BspPI GGATC 3 cut(s) 665, 854, 867
BspT104I TTCGAA 1 cut(s) 494
BspT107I GGYRCC 2 cut(s) 134, 1496
BsrDI GCAATG 1 cut(s) 1413
BsrFI RCCGGY 1 cut(s) 213
BsrI ACTGG 2 cut(s) 662, 863
BssAI RCCGGY 1 cut(s) 213
BssECI CCNNGG 3 cut(s) 543, 1233, 1355
BssMI GATC 3 cut(s) 145, 670, 859
BssT1I CCWWGG 2 cut(s) 543, 1233
Bst4CI ACNGT 1 cut(s) 729
Bst6I CTCTTC 2 cut(s) 62, 68
BstAPI GCANNNNNTGC 2 cut(s) 526, 1346
BstBI TTCGAA 1 cut(s) 494
BstC8I GCNNGC 4 cut(s) 94, 396, 400, 1512
BstDEI CTNAG 8 cut(s) 80, 333, 425, 711, 795, 887, 965, 1269
BstDSI CCRYGG 1 cut(s) 1233
BstENI CCTNNNNNAGG 1 cut(s) 129
BstF5I GGATG 2 cut(s) 1018, 1153
BstFNI CGCG 1 cut(s) 1103
BstKTI GATC 3 cut(s) 148, 673, 862
BstMAI GTCTC 1 cut(s) 1200
BstMBI GATC 3 cut(s) 145, 670, 859
BstMWI GCNNNNNNNGC 4 cut(s) 391, 526, 1223, 1346
BstNSI RCATGY 2 cut(s) 36, 964
BstSFI CTRYAG 1 cut(s) 1134
BstSLI GKGCMC 1 cut(s) 759
BstUI CGCG 1 cut(s) 1103
BstV1I GCAGC 5 cut(s) 246, 394, 448, 631, 778
BstX2I RGATCY 1 cut(s) 859
BstYI RGATCY 1 cut(s) 859
BsuRI GGCC 5 cut(s) 204, 629, 716, 1226, 1354
BtgI CCRYGG 1 cut(s) 1233
BtgZI GCGATG 1 cut(s) 1321
BtsCI GGATG 2 cut(s) 1018, 1153
BtsIMutI CAGTG 1 cut(s) 870
Cac8I GCNNGC 4 cut(s) 94, 396, 400, 1512
CaiI CAGNNNCTG 1 cut(s) 1142
Cfr10I RCCGGY 1 cut(s) 213
Cfr13I GGNCC 4 cut(s) 203, 1352, 1359, 1470
Csp6I GTAC 1 cut(s) 540
CviQI GTAC 1 cut(s) 540
DdeI CTNAG 8 cut(s) 80, 333, 425, 711, 795, 887, 965, 1269
DpnI GATC 3 cut(s) 147, 672, 861
DpnII GATC 3 cut(s) 145, 670, 859
Eam1104I CTCTTC 2 cut(s) 62, 68
EarI CTCTTC 2 cut(s) 62, 68
Eco130I CCWWGG 2 cut(s) 543, 1233
Eco147I AGGCCT 1 cut(s) 629
Eco47I GGWCC 2 cut(s) 1359, 1470
Eco88I CYCGRG 1 cut(s) 186
EcoNI CCTNNNNNAGG 1 cut(s) 129
EcoO109I RGGNCCY 1 cut(s) 1352
EcoRI GAATTC 1 cut(s) 496
EcoT14I CCWWGG 2 cut(s) 543, 1233
EcoT22I ATGCAT 1 cut(s) 1092
ErhI CCWWGG 2 cut(s) 543, 1233
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 1 cut(s) 1388
FbaI TGATCA 1 cut(s) 145
Fnu4HI GCNGC 5 cut(s) 235, 383, 462, 645, 792
FokI GGATG 2 cut(s) 1025, 1160
Fsp4HI GCNGC 5 cut(s) 235, 383, 462, 645, 792
FspBI CTAG 2 cut(s) 788, 1367
GluI GCNGC 5 cut(s) 235, 383, 462, 645, 792
HaeIII GGCC 5 cut(s) 204, 629, 716, 1226, 1354
HapII CCGG 2 cut(s) 214, 1429
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 7 cut(s) 84, 421, 548, 638, 1027, 1034, 1319
HpaII CCGG 2 cut(s) 214, 1429
HphI GGTGA 6 cut(s) 16, 140, 203, 733, 1314, 1471
Hpy166II GTNNAC 2 cut(s) 757, 1479
Hpy188III TCNNGA 5 cut(s) 280, 679, 1031, 1079, 1181
Hpy8I GTNNAC 2 cut(s) 757, 1479
HpyAV CCTTC 5 cut(s) 37, 194, 619, 1106, 1493
HpyCH4III ACNGT 1 cut(s) 729
HpyCH4IV ACGT 2 cut(s) 666, 1251
HpyF10VI GCNNNNNNNGC 4 cut(s) 391, 526, 1223, 1346
HpyF3I CTNAG 8 cut(s) 80, 333, 425, 711, 795, 887, 965, 1269
HpySE526I ACGT 2 cut(s) 666, 1251
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 3 cut(s) 145, 670, 859
Lsp1109I GCAGC 5 cut(s) 246, 394, 448, 631, 778
LweI GCATC 3 cut(s) 1060, 1077, 1255
MaeI CTAG 2 cut(s) 788, 1367
MaeII ACGT 2 cut(s) 666, 1251
MaeIII GTNAC 6 cut(s) 251, 308, 662, 739, 812, 1459
MalI GATC 3 cut(s) 147, 672, 861
MboI GATC 3 cut(s) 145, 670, 859
MboII GAAGA 9 cut(s) 13, 49, 55, 79, 946, 1169, 1274, 1408, 1417
MflI RGATCY 1 cut(s) 859
MhlI GDGCHC 1 cut(s) 759
MluCI AATT 9 cut(s) 407, 476, 496, 583, 897, 904, 1049, 1331, 1436
MlyI GAGTC 1 cut(s) 1028
MmeI TCCRAC 3 cut(s) 544, 1000, 1303
Mph1103I ATGCAT 1 cut(s) 1092
MseI TTAA 4 cut(s) 363, 474, 599, 723
MslI CAYNNNNRTG 2 cut(s) 946, 1491
MspA1I CMGCKG 2 cut(s) 438, 644
MspI CCGG 2 cut(s) 214, 1429
Mva1269I GAATGC 1 cut(s) 1248
MvnI CGCG 1 cut(s) 1103
MwoI GCNNNNNNNGC 4 cut(s) 391, 526, 1223, 1346
NcoI CCATGG 1 cut(s) 1233
NdeII GATC 3 cut(s) 145, 670, 859
NlaIV GGNNCC 3 cut(s) 136, 861, 1498
NmuCI GTSAC 4 cut(s) 251, 308, 739, 812
NsiI ATGCAT 1 cut(s) 1092
NspI RCATGY 2 cut(s) 36, 964
NspV TTCGAA 1 cut(s) 494
PceI AGGCCT 1 cut(s) 629
PciI ACATGT 1 cut(s) 32
PctI GAATGC 1 cut(s) 1248
PfeI GAWTC 6 cut(s) 84, 421, 548, 638, 1027, 1319
PkrI GCNGC 5 cut(s) 236, 384, 463, 646, 793
PleI GAGTC 1 cut(s) 1028
PpsI GAGTC 1 cut(s) 1028
PscI ACATGT 1 cut(s) 32
PsiI TTATAA 1 cut(s) 767
PspN4I GGNNCC 3 cut(s) 136, 861, 1498
PspPI GGNCC 4 cut(s) 203, 1352, 1359, 1470
PstI CTGCAG 1 cut(s) 1138
PstNI CAGNNNCTG 1 cut(s) 1142
PsuI RGATCY 1 cut(s) 859
PvuII CAGCTG 2 cut(s) 438, 644
RsaI GTAC 1 cut(s) 541
RsaNI GTAC 1 cut(s) 540
RseI CAYNNNNRTG 2 cut(s) 946, 1491
SaqAI TTAA 4 cut(s) 363, 474, 599, 723
SatI GCNGC 5 cut(s) 235, 383, 462, 645, 792
Sau3AI GATC 3 cut(s) 145, 670, 859
Sau96I GGNCC 4 cut(s) 203, 1352, 1359, 1470
SchI GAGTC 1 cut(s) 1028
SduI GDGCHC 1 cut(s) 759
SfaNI GCATC 3 cut(s) 1060, 1077, 1255
SfcI CTRYAG 1 cut(s) 1134
SfuI TTCGAA 1 cut(s) 494
SinI GGWCC 2 cut(s) 1359, 1470
SmiMI CAYNNNNRTG 2 cut(s) 946, 1491
SmlI CTYRAG 2 cut(s) 278, 1120
SmoI CTYRAG 2 cut(s) 278, 1120
Sse9I AATT 9 cut(s) 407, 476, 496, 583, 897, 904, 1049, 1331, 1436
SseBI AGGCCT 1 cut(s) 629
SspI AATATT 1 cut(s) 703
SspMI CTAG 2 cut(s) 788, 1367
StuI AGGCCT 1 cut(s) 629
StyI CCWWGG 2 cut(s) 543, 1233
TaaI ACNGT 1 cut(s) 729
TaiI ACGT 2 cut(s) 669, 1254
TaqI TCGA 4 cut(s) 259, 413, 494, 1296
TasI AATT 9 cut(s) 407, 476, 496, 583, 897, 904, 1049, 1331, 1436
TfiI GAWTC 6 cut(s) 84, 421, 548, 638, 1027, 1319
Tru1I TTAA 4 cut(s) 363, 474, 599, 723
Tru9I TTAA 4 cut(s) 363, 474, 599, 723
TscAI CASTG 1 cut(s) 870
TseFI GTSAC 4 cut(s) 251, 308, 739, 812
TseI GCWGC 5 cut(s) 234, 382, 461, 644, 791
Tsp45I GTSAC 4 cut(s) 251, 308, 739, 812
TspRI CASTG 1 cut(s) 870
VneI GTGCAC 1 cut(s) 755
VpaK11BI GGWCC 2 cut(s) 1359, 1470
XagI CCTNNNNNAGG 1 cut(s) 129
XapI RAATTY 6 cut(s) 407, 476, 496, 904, 1049, 1331
XceI RCATGY 2 cut(s) 36, 964
XspI CTAG 2 cut(s) 788, 1367
Zsp2I ATGCAT 1 cut(s) 1092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.