MD02G1200500.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000330
Physical Location & Seq
Reverse (-)
19731218 .. 19732337
1120 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1200500.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGGGTTTTTCCTTATCATCTTCACCTCCATACTTATGTTTCTCTTCCTAACTATGTCTTTGTTCATACTCAAAATCTTCACTGGAAAGTTTATCCGAGACCCGAACTACCTTATCTTCCACATGTTCTTATACTTCAACAGAGTCTACCACTACCAAATCAAAGTTGCCAAAACACACTCAACTTTCCAGCTTCTAGTCCCGGACCACAGCGAAGTGTACATGGCTGACATGCGCAAAATTGAGCATGTTCTGAAAACCAACTTTGCCAGTTACTCGAAAGGCGCCTTCACTCAAAGTATTCTGTCGGATATTTTTGGCCAAGGGATATTTGTTGTTGATGGAGAAAAATGGAAGCAGCAGAGGAAGCTTGCGAGCTTCGAGTTTTCAACAAGGGTGCTCAGAGATGCTAGCTGCTCTGTGTTTAGAAGAAATGCTGCCAAACTGGTTAGAATTGTTTTTGAAATTTCAAGTTCCAGAGGAGTTTTTGATATGCAGGATTTGCTTATGAGATGTACCTTGGATTCCATATTCAAAGTCGGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

183

Amino Acids

21.35

Weight (kDa)

9.73

Isoelectric Point (pI)

31.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 182 1.1e-11 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44890 AT2G44890 AT2G45510
fragaria_vesca FvH4_7g12200 FvH4_7g12250 FvH4_7g12281 FvH4_7g12281 FvH4_7g12281 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12300 FvH4_7g12310
malus_domestica MD02G1200000.v1.1 MD02G1200100.v1.1 MD02G1200300.v1.1 MD02G1200400.v1.1 MD02G1200500.v1.1 MD02G1200600.v1.1 MD02G1200700.v1.1
prunus_persica Prupe.2G148400_v2.0.a1 Prupe.2G148500_v2.0.a1 Prupe.2G148600_v2.0.a1 Prupe.2G148700_v2.0.a1
pyrus_communis pycom02g16390
rosa_chinensis RchiOBHm_Chr1g0350941 RchiOBHm_Chr1g0350951 RchiOBHm_Chr1g0350981 RchiOBHm_Chr1g0350991 RchiOBHm_Chr1g0351001 RchiOBHm_Chr1g0351031 RchiOBHm_Chr1g0351061 RchiOBHm_Chr1g0351071 RchiOBHm_Chr1g0351081 RchiOBHm_Chr1g0351091 RchiOBHm_Chr1g0351101 RchiOBHm_Chr1g0375431 RchiOBHm_Chr2g0142281
rosa_laevigata RLG00000026651 RLG00000028432 RLG00000028433 RLG00000028435 RLG00000028436 RLG00000028437 RLG00000028438 RLG00000028439
rosa_multiflora Rmu_co7992102.1_g000001 Rmu_sc0000429.1_g000085 Rmu_sc0002665.1_g000014 Rmu_sc0002665.1_g000018 Rmu_sc0006087.1_g000001 Rmu_sc0006458.1_g000011 Rmu_sc0008688.1_g000002 Rmu_sc0008688.1_g000006 Rmu_sc0009646.1_g000012 Rmu_sc0032693.1_g000003
rosa_roxburghii Rroxscaffold_4G00304080 Rroxscaffold_4G00304120 Rroxscaffold_4G00304150 Rroxscaffold_4G00304170 Rroxscaffold_4G00304180 Rroxscaffold_4G00304190 Rroxscaffold_4G00304210 Rroxscaffold_4G00304230 Rroxscaffold_4G00304280
rosa_rugosa Rorug01G0075700 Rorug01G0215400 Rorug01G0216000 Rorug01G0216100 Rorug01G0216200 Rorug01G0216300 Rorug01G0216300 Rorug01G0216300 Rorug02G0238800 Rorug04G0006700 Rorug06G0023400
rosa_samantha Rh1AG230300 Rh1AG230400 Rh1AG230700 Rh1AG230800 Rh1BG200100 Rh1BG200200 Rh1BG200600 Rh1DG227900 Rh1DG228000 Rh1DG228200
rosa_wichuraiana Rw0G010880 Rw1G019960 Rw1G019970 Rw1G019990 Rw1G020000 Rw1G020010 Rw1G020040 Rw1G020050 Rw1G020060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 236
AccB1I GGYRCC 1 cut(s) 284
AccI GTMKAC 1 cut(s) 147
AcoI YGGCCR 1 cut(s) 319
AcsI RAATTY 1 cut(s) 465
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 2 cut(s) 221, 517
AflIII ACRYGT 1 cut(s) 123
AgsI TTSAA 5 cut(s) 139, 390, 464, 471, 535
AluBI AGCT 4 cut(s) 193, 370, 378, 414
AluI AGCT 4 cut(s) 193, 370, 378, 414
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 1 cut(s) 93
AoxI GGCC 1 cut(s) 319
ApeKI GCWGC 3 cut(s) 358, 414, 437
ApoI RAATTY 1 cut(s) 465
AspLEI GCGC 2 cut(s) 237, 287
AspS9I GGNCC 1 cut(s) 205
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 1 cut(s) 16
AsuNHI GCTAGC 1 cut(s) 410
AvaII GGWCC 1 cut(s) 205
BalI TGGCCA 1 cut(s) 321
BanI GGYRCC 1 cut(s) 284
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 3 cut(s) 370, 401, 424
BccI CCATC 1 cut(s) 335
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 93
BfaI CTAG 2 cut(s) 197, 411
BfoI RGCGCY 1 cut(s) 288
BisI GCNGC 3 cut(s) 359, 415, 438
BlsI GCNGC 3 cut(s) 360, 416, 439
Bme1390I CCNGG 1 cut(s) 203
Bme18I GGWCC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 1 cut(s) 286
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 397
BmtI GCTAGC 1 cut(s) 414
BpuMI CCSGG 1 cut(s) 203
BsaHI GRCGYC 1 cut(s) 285
BsaI GGTCTC 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 322, 519
Bse1I ACTGG 3 cut(s) 88, 270, 450
BseDI CCNNGG 2 cut(s) 322, 519
BseMII CTCAG 1 cut(s) 415
BseNI ACTGG 3 cut(s) 88, 270, 450
BseRI GAGGAG 1 cut(s) 495
BseXI GCAGC 3 cut(s) 370, 401, 424
BshFI GGCC 1 cut(s) 321
BshNI GGYRCC 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 402
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 1 cut(s) 185
BsmAI GTCTC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 185
BsnI GGCC 1 cut(s) 321
Bso31I GGTCTC 1 cut(s) 93
Bsp1286I GDGCHC 1 cut(s) 402
Bsp1407I TGTACA 1 cut(s) 219
BspANI GGCC 1 cut(s) 321
BspCNI CTCAG 1 cut(s) 414
BspLI GGNNCC 1 cut(s) 286
BspOI GCTAGC 1 cut(s) 414
BspT107I GGYRCC 1 cut(s) 284
BspTNI GGTCTC 1 cut(s) 93
BsrGI TGTACA 1 cut(s) 219
BsrI ACTGG 3 cut(s) 88, 270, 450
BssECI CCNNGG 2 cut(s) 322, 519
BssNI GRCGYC 1 cut(s) 285
BssT1I CCWWGG 2 cut(s) 322, 519
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 1 cut(s) 285
BstAPI GCANNNNNTGC 1 cut(s) 502
BstAUI TGTACA 1 cut(s) 219
BstC8I GCNNGC 3 cut(s) 372, 376, 412
BstDEI CTNAG 1 cut(s) 401
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 2 cut(s) 237, 287
BstMAI GTCTC 1 cut(s) 93
BstMWI GCNNNNNNNGC 2 cut(s) 367, 502
BstNSI RCATGY 3 cut(s) 127, 235, 251
BstSCI CCNGG 1 cut(s) 201
BstV1I GCAGC 3 cut(s) 370, 401, 424
BsuRI GGCC 1 cut(s) 321
BtsIMutI CAGTG 1 cut(s) 81
Cac8I GCNNGC 3 cut(s) 372, 376, 412
CfoI GCGC 2 cut(s) 237, 287
Cfr13I GGNCC 1 cut(s) 205
Csp6I GTAC 2 cut(s) 220, 516
CviAII CATG 4 cut(s) 124, 223, 232, 248
CviJI RGCY 6 cut(s) 193, 227, 321, 370, 378, 414
CviKI_1 RGCY 6 cut(s) 193, 227, 321, 370, 378, 414
CviQI GTAC 2 cut(s) 220, 516
DdeI CTNAG 1 cut(s) 401
DinI GGCGCC 1 cut(s) 286
EaeI YGGCCR 1 cut(s) 319
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
Eco130I CCWWGG 2 cut(s) 322, 519
Eco31I GGTCTC 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 205
EcoT14I CCWWGG 2 cut(s) 322, 519
EgeI GGCGCC 1 cut(s) 286
EheI GGCGCC 1 cut(s) 286
ErhI CCWWGG 2 cut(s) 322, 519
FaeI CATG 4 cut(s) 127, 226, 235, 251
FaqI GGGAC 1 cut(s) 185
FatI CATG 4 cut(s) 123, 222, 231, 247
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 3 cut(s) 359, 415, 438
Fsp4HI GCNGC 3 cut(s) 359, 415, 438
FspBI CTAG 2 cut(s) 197, 411
FspI TGCGCA 1 cut(s) 236
GlaI GCGC 2 cut(s) 236, 286
GluI GCNGC 3 cut(s) 359, 415, 438
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 1 cut(s) 321
HapII CCGG 1 cut(s) 203
HhaI GCGC 2 cut(s) 237, 287
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 4 cut(s) 127, 226, 235, 251
Hin6I GCGC 2 cut(s) 235, 285
HinP1I GCGC 2 cut(s) 235, 285
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 2 cut(s) 144, 524
HpaII CCGG 1 cut(s) 203
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 148, 220
Hpy188I TCNGA 4 cut(s) 98, 255, 310, 404
Hpy188III TCNNGA 1 cut(s) 477
Hpy8I GTNNAC 2 cut(s) 148, 220
HpyAV CCTTC 1 cut(s) 298
HpyCH4V TGCA 1 cut(s) 496
HpyF10VI GCNNNNNNNGC 2 cut(s) 367, 502
HpyF3I CTNAG 1 cut(s) 401
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 4 cut(s) 127, 226, 235, 251
HspAI GCGC 2 cut(s) 235, 285
KasI GGCGCC 1 cut(s) 284
LpnPI CCDG 7 cut(s) 69, 203, 216, 283, 431, 482, 490
Lsp1109I GCAGC 3 cut(s) 370, 401, 424
LweI GCATC 1 cut(s) 397
MaeI CTAG 2 cut(s) 197, 411
MaeIII GTNAC 1 cut(s) 272
MboII GAAGA 5 cut(s) 13, 37, 70, 109, 441
MhlI GDGCHC 1 cut(s) 402
MlsI TGGCCA 1 cut(s) 321
MluCI AATT 3 cut(s) 240, 453, 465
MluNI TGGCCA 1 cut(s) 321
Mly113I GGCGCC 1 cut(s) 285
MlyI GAGTC 1 cut(s) 153
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 3 cut(s) 37, 357, 473
Mox20I TGGCCA 1 cut(s) 321
MscI TGGCCA 1 cut(s) 321
MslI CAYNNNNRTG 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 321
MspI CCGG 1 cut(s) 203
MspR9I CCNGG 1 cut(s) 203
MwoI GCNNNNNNNGC 2 cut(s) 367, 502
NarI GGCGCC 1 cut(s) 285
NciI CCSGG 1 cut(s) 203
NheI GCTAGC 1 cut(s) 410
NlaIII CATG 4 cut(s) 127, 226, 235, 251
NlaIV GGNNCC 1 cut(s) 286
NsbI TGCGCA 1 cut(s) 236
NspI RCATGY 3 cut(s) 127, 235, 251
PciI ACATGT 1 cut(s) 123
PfeI GAWTC 1 cut(s) 524
PfoI TCCNGGA 1 cut(s) 201
PkrI GCNGC 3 cut(s) 360, 416, 439
PleI GAGTC 1 cut(s) 152
PluTI GGCGCC 1 cut(s) 288
PpsI GAGTC 1 cut(s) 152
PscI ACATGT 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 286
PspPI GGNCC 1 cut(s) 205
RsaI GTAC 2 cut(s) 221, 517
RsaNI GTAC 2 cut(s) 220, 516
RseI CAYNNNNRTG 1 cut(s) 35
SatI GCNGC 3 cut(s) 359, 415, 438
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 1 cut(s) 203
SduI GDGCHC 1 cut(s) 402
SetI ASST 7 cut(s) 29, 114, 195, 372, 380, 416, 521
SfaNI GCATC 1 cut(s) 397
SfoI GGCGCC 1 cut(s) 286
SinI GGWCC 1 cut(s) 205
SmiMI CAYNNNNRTG 1 cut(s) 35
Sse9I AATT 3 cut(s) 240, 453, 465
SspDI GGCGCC 1 cut(s) 284
SspMI CTAG 2 cut(s) 197, 411
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 2 cut(s) 322, 519
TaqI TCGA 2 cut(s) 278, 381
TasI AATT 3 cut(s) 240, 453, 465
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 1 cut(s) 524
TscAI CASTG 1 cut(s) 88
TseI GCWGC 3 cut(s) 358, 414, 437
TspDTI ATGAA 1 cut(s) 55
TspRI CASTG 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 205
XapI RAATTY 1 cut(s) 465
XceI RCATGY 3 cut(s) 127, 235, 251
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 2 cut(s) 197, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.