cytochrome P450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 142 |
| Acc36I | ACCTGC | 1 cut(s) | 2069 |
| AccB1I | GGYRCC | 1 cut(s) | 1088 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1877 |
| AccI | GTMKAC | 2 cut(s) | 522, 663 |
| AccII | CGCG | 3 cut(s) | 1039, 1832, 2086 |
| AciI | CCGC | 5 cut(s) | 1507, 1832, 1865, 2084, 2086 |
| AclWI | GGATC | 4 cut(s) | 158, 338, 351, 1394 |
| AcoI | YGGCCR | 2 cut(s) | 1123, 2169 |
| AcsI | RAATTY | 6 cut(s) | 753, 779, 1630, 1778, 2034, 2060 |
| AcuI | CTGAAG | 3 cut(s) | 225, 987, 1818 |
| AcyI | GRCGYC | 1 cut(s) | 1089 |
| AfaI | GTAC | 6 cut(s) | 249, 443, 1025, 1079, 1270, 1725 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 235, 911, 1877 |
| AflII | CTTAAG | 1 cut(s) | 1849 |
| AflIII | ACRYGT | 1 cut(s) | 927 |
| AgsI | TTSAA | 8 cut(s) | 93, 181, 778, 943, 1288, 1429, 2059, 2164 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1555 |
| AjnI | CCWGG | 1 cut(s) | 1073 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1421, 1453 |
| AluBI | AGCT | 5 cut(s) | 530, 1174, 1182, 1218, 2151 |
| AluI | AGCT | 5 cut(s) | 530, 1174, 1182, 1218, 2151 |
| Alw21I | GWGCWC | 1 cut(s) | 1488 |
| Alw26I | GTCTC | 3 cut(s) | 1838, 1929, 2234 |
| Alw44I | GTGCAC | 1 cut(s) | 1484 |
| AlwI | GGATC | 4 cut(s) | 158, 338, 351, 1394 |
| Ama87I | CYCGRG | 1 cut(s) | 347 |
| AoxI | GGCC | 7 cut(s) | 120, 672, 692, 1123, 1356, 1443, 2169 |
| ApaLI | GTGCAC | 1 cut(s) | 1484 |
| ApeKI | GCWGC | 5 cut(s) | 209, 1162, 1218, 1241, 1500 |
| ApoI | RAATTY | 6 cut(s) | 753, 779, 1630, 1778, 2034, 2060 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1655, 1687 |
| AspLEI | GCGC | 4 cut(s) | 156, 1041, 1091, 1837 |
| AspS9I | GGNCC | 3 cut(s) | 1009, 2081, 2199 |
| AsuC2I | CCSGG | 1 cut(s) | 1007 |
| AsuHPI | GGTGA | 6 cut(s) | 762, 805, 862, 1945, 2043, 2200 |
| AsuNHI | GCTAGC | 1 cut(s) | 530 |
| AvaI | CYCGRG | 1 cut(s) | 347 |
| AvaII | GGWCC | 3 cut(s) | 1009, 2081, 2199 |
| BaeGI | GKGCMC | 1 cut(s) | 1488 |
| BalI | TGGCCA | 1 cut(s) | 1125 |
| BamHI | GGATCC | 1 cut(s) | 343 |
| BanI | GGYRCC | 1 cut(s) | 1088 |
| BbsI | GAAGAC | 1 cut(s) | 913 |
| Bbv12I | GWGCWC | 1 cut(s) | 1488 |
| BbvCI | CCTCAGC | 1 cut(s) | 717 |
| BbvI | GCAGC | 5 cut(s) | 221, 1174, 1205, 1228, 1512 |
| BceAI | ACGGC | 1 cut(s) | 1044 |
| BciT130I | CCWGG | 1 cut(s) | 1075 |
| BcnI | CCSGG | 1 cut(s) | 1007 |
| BcoDI | GTCTC | 3 cut(s) | 1838, 1929, 2234 |
| BfaI | CTAG | 3 cut(s) | 531, 1001, 1808 |
| BfmI | CTRYAG | 1 cut(s) | 2194 |
| BfoI | RGCGCY | 3 cut(s) | 157, 1092, 1838 |
| BfrI | CTTAAG | 1 cut(s) | 1849 |
| BfuAI | ACCTGC | 1 cut(s) | 2069 |
| BisI | GCNGC | 6 cut(s) | 210, 1163, 1219, 1242, 1501, 1833 |
| BlpI | GCTNAGC | 1 cut(s) | 1691 |
| BlsI | GCNGC | 6 cut(s) | 211, 1164, 1220, 1243, 1502, 1834 |
| Bme1390I | CCNGG | 2 cut(s) | 1007, 1075 |
| Bme18I | GGWCC | 3 cut(s) | 1009, 2081, 2199 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1555 |
| BmeT110I | CYCGRG | 1 cut(s) | 347 |
| BmgT120I | GGNCC | 3 cut(s) | 1009, 2081, 2199 |
| BmiI | GGNNCC | 4 cut(s) | 345, 1090, 1438, 1869 |
| BmrFI | CCNGG | 2 cut(s) | 1007, 1075 |
| BmsI | GCATC | 5 cut(s) | 21, 286, 553, 703, 1984 |
| BmtI | GCTAGC | 1 cut(s) | 534 |
| BpiI | GAAGAC | 1 cut(s) | 913 |
| BpmI | CTGGAG | 1 cut(s) | 1592 |
| Bpu10I | CCTNAGC | 2 cut(s) | 717, 2147 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1691 |
| BpuMI | CCSGG | 1 cut(s) | 1007 |
| Bsa29I | ATCGAT | 2 cut(s) | 1473, 1875 |
| BsaBI | GATNNNNATC | 1 cut(s) | 567 |
| BsaHI | GRCGYC | 1 cut(s) | 1089 |
| BsaI | GGTCTC | 1 cut(s) | 1838 |
| BsaJI | CCNNGG | 7 cut(s) | 681, 1126, 1272, 1432, 1440, 1962, 2084 |
| BsaWI | WCCGGW | 1 cut(s) | 872 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 26, 56, 645, 675, 1926, 1956 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 235, 911, 1877 |
| Bse118I | RCCGGY | 2 cut(s) | 2115, 2185 |
| Bse1I | ACTGG | 3 cut(s) | 1254, 1575, 1656 |
| Bse3DI | GCAATG | 1 cut(s) | 1312 |
| Bse8I | GATNNNNATC | 1 cut(s) | 567 |
| BseBI | CCWGG | 1 cut(s) | 1075 |
| BseCI | ATCGAT | 2 cut(s) | 1473, 1875 |
| BseDI | CCNNGG | 7 cut(s) | 681, 1126, 1272, 1432, 1440, 1962, 2084 |
| BseGI | GGATG | 4 cut(s) | 244, 502, 882, 1744 |
| BseJI | GATNNNNATC | 1 cut(s) | 567 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 235, 911, 1877 |
| BseMI | GCAATG | 1 cut(s) | 1312 |
| BseMII | CTCAG | 5 cut(s) | 362, 708, 874, 1604, 1705 |
| BseNI | ACTGG | 3 cut(s) | 1254, 1575, 1656 |
| BseRI | GAGGAG | 1 cut(s) | 1535 |
| BseSI | GKGCMC | 1 cut(s) | 1488 |
| BseXI | GCAGC | 5 cut(s) | 221, 1174, 1205, 1228, 1512 |
| BsgI | GTGCAG | 3 cut(s) | 627, 1359, 1908 |
| Bsh1236I | CGCG | 3 cut(s) | 1039, 1832, 2086 |
| BshFI | GGCC | 7 cut(s) | 122, 674, 694, 1125, 1358, 1445, 2171 |
| BshNI | GGYRCC | 1 cut(s) | 1088 |
| BshVI | ATCGAT | 2 cut(s) | 1473, 1875 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1488 |
| BsiHKCI | CYCGRG | 1 cut(s) | 347 |
| BsiSI | CCGG | 5 cut(s) | 873, 994, 1007, 2116, 2186 |
| BslFI | GGGAC | 2 cut(s) | 989, 1354 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 235, 911, 1877 |
| BsmAI | GTCTC | 3 cut(s) | 1838, 1929, 2234 |
| BsmFI | GGGAC | 2 cut(s) | 989, 1354 |
| BsmI | GAATGC | 3 cut(s) | 1346, 1821, 1977 |
| BsnI | GGCC | 7 cut(s) | 122, 674, 694, 1125, 1358, 1445, 2171 |
| Bso31I | GGTCTC | 1 cut(s) | 1838 |
| BsoBI | CYCGRG | 1 cut(s) | 347 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1488 |
| Bsp1407I | TGTACA | 4 cut(s) | 247, 441, 1023, 1723 |
| Bsp143I | GATC | 3 cut(s) | 163, 343, 1399 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1691 |
| Bsp19I | CCATGG | 2 cut(s) | 681, 1962 |
| BspACI | CCGC | 5 cut(s) | 1507, 1832, 1865, 2084, 2086 |
| BspANI | GGCC | 7 cut(s) | 122, 674, 694, 1125, 1358, 1445, 2171 |
| BspCNI | CTCAG | 5 cut(s) | 363, 709, 873, 1605, 1704 |
| BspDI | ATCGAT | 2 cut(s) | 1473, 1875 |
| BspFNI | CGCG | 3 cut(s) | 1039, 1832, 2086 |
| BspHI | TCATGA | 2 cut(s) | 592, 1350 |
| BspLI | GGNNCC | 4 cut(s) | 345, 1090, 1438, 1869 |
| BspMI | ACCTGC | 1 cut(s) | 2069 |
| BspOI | GCTAGC | 1 cut(s) | 534 |
| BspPI | GGATC | 4 cut(s) | 158, 338, 351, 1394 |
| BspT107I | GGYRCC | 1 cut(s) | 1088 |
| BspTI | CTTAAG | 1 cut(s) | 1849 |
| BspTNI | GGTCTC | 1 cut(s) | 1838 |
| BsrDI | GCAATG | 1 cut(s) | 1312 |
| BsrFI | RCCGGY | 2 cut(s) | 2115, 2185 |
| BsrGI | TGTACA | 4 cut(s) | 247, 441, 1023, 1723 |
| BsrI | ACTGG | 3 cut(s) | 1254, 1575, 1656 |
| BssAI | RCCGGY | 2 cut(s) | 2115, 2185 |
| BssECI | CCNNGG | 7 cut(s) | 681, 1126, 1272, 1432, 1440, 1962, 2084 |
| BssMI | GATC | 3 cut(s) | 163, 343, 1399 |
| BssNAI | GTATAC | 1 cut(s) | 664 |
| BssNI | GRCGYC | 1 cut(s) | 1089 |
| BssT1I | CCWWGG | 5 cut(s) | 681, 1126, 1272, 1432, 1962 |
| Bst1107I | GTATAC | 1 cut(s) | 664 |
| Bst2UI | CCWGG | 1 cut(s) | 1075 |
| Bst4CI | ACNGT | 5 cut(s) | 844, 919, 1016, 1557, 1671 |
| BstACI | GRCGYC | 1 cut(s) | 1089 |
| BstAFI | CTTAAG | 1 cut(s) | 1849 |
| BstAUI | TGTACA | 4 cut(s) | 247, 441, 1023, 1723 |
| BstC8I | GCNNGC | 6 cut(s) | 403, 532, 1176, 1180, 1505, 1645 |
| BstDEI | CTNAG | 9 cut(s) | 37, 215, 371, 717, 860, 1205, 1613, 1691, 2147 |
| BstDSI | CCRYGG | 3 cut(s) | 681, 1962, 2084 |
| BstEII | GGTNACC | 1 cut(s) | 2188 |
| BstF5I | GGATG | 4 cut(s) | 244, 502, 882, 1744 |
| BstFNI | CGCG | 3 cut(s) | 1039, 1832, 2086 |
| BstH2I | RGCGCY | 3 cut(s) | 157, 1092, 1838 |
| BstHHI | GCGC | 4 cut(s) | 156, 1041, 1091, 1837 |
| BstKTI | GATC | 3 cut(s) | 166, 346, 1402 |
| BstMAI | GTCTC | 3 cut(s) | 1838, 1929, 2234 |
| BstMBI | GATC | 3 cut(s) | 163, 343, 1399 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 1171, 1513, 1522, 2075, 2157 |
| BstNI | CCWGG | 1 cut(s) | 1075 |
| BstNSI | RCATGY | 4 cut(s) | 448, 931, 1055, 1690 |
| BstPI | GGTNACC | 1 cut(s) | 2188 |
| BstSCI | CCNGG | 2 cut(s) | 1005, 1073 |
| BstSFI | CTRYAG | 1 cut(s) | 2194 |
| BstSLI | GKGCMC | 1 cut(s) | 1488 |
| BstUI | CGCG | 3 cut(s) | 1039, 1832, 2086 |
| BstV1I | GCAGC | 5 cut(s) | 221, 1174, 1205, 1228, 1512 |
| BstV2I | GAAGAC | 1 cut(s) | 913 |
| BstX2I | RGATCY | 1 cut(s) | 343 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2019 |
| BstYI | RGATCY | 1 cut(s) | 343 |
| BstZ17I | GTATAC | 1 cut(s) | 664 |
| Bsu15I | ATCGAT | 2 cut(s) | 1473, 1875 |
| BsuRI | GGCC | 7 cut(s) | 122, 674, 694, 1125, 1358, 1445, 2171 |
| BsuTUI | ATCGAT | 2 cut(s) | 1473, 1875 |
| BtgI | CCRYGG | 3 cut(s) | 681, 1962, 2084 |
| BtgZI | GCGATG | 1 cut(s) | 2150 |
| BtsCI | GGATG | 4 cut(s) | 244, 502, 882, 1744 |
| BtsIMutI | CAGTG | 5 cut(s) | 915, 1021, 1376, 1663, 1667 |
| BveI | ACCTGC | 1 cut(s) | 2069 |
| Cac8I | GCNNGC | 6 cut(s) | 403, 532, 1176, 1180, 1505, 1645 |
| CciI | TCATGA | 2 cut(s) | 592, 1350 |
| CfoI | GCGC | 4 cut(s) | 156, 1041, 1091, 1837 |
| Cfr10I | RCCGGY | 2 cut(s) | 2115, 2185 |
| Cfr13I | GGNCC | 3 cut(s) | 1009, 2081, 2199 |
| Cfr42I | CCGCGG | 1 cut(s) | 2087 |
| ClaI | ATCGAT | 2 cut(s) | 1473, 1875 |
| Csp6I | GTAC | 6 cut(s) | 248, 442, 1024, 1078, 1269, 1724 |
| CviQI | GTAC | 6 cut(s) | 248, 442, 1024, 1078, 1269, 1724 |
| DdeI | CTNAG | 9 cut(s) | 37, 215, 371, 717, 860, 1205, 1613, 1691, 2147 |
| DinI | GGCGCC | 1 cut(s) | 1090 |
| DpnI | GATC | 3 cut(s) | 165, 345, 1401 |
| DpnII | GATC | 3 cut(s) | 163, 343, 1399 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 142 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1555 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 142 |
| EaeI | YGGCCR | 2 cut(s) | 1123, 2169 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1555 |
| Eco130I | CCWWGG | 5 cut(s) | 681, 1126, 1272, 1432, 1962 |
| Eco147I | AGGCCT | 3 cut(s) | 694, 1358, 1445 |
| Eco31I | GGTCTC | 1 cut(s) | 1838 |
| Eco47I | GGWCC | 3 cut(s) | 1009, 2081, 2199 |
| Eco57I | CTGAAG | 3 cut(s) | 225, 987, 1818 |
| Eco88I | CYCGRG | 1 cut(s) | 347 |
| Eco91I | GGTNACC | 1 cut(s) | 2188 |
| EcoO65I | GGTNACC | 1 cut(s) | 2188 |
| EcoRI | GAATTC | 1 cut(s) | 1778 |
| EcoRII | CCWGG | 1 cut(s) | 1073 |
| EcoT14I | CCWWGG | 5 cut(s) | 681, 1126, 1272, 1432, 1962 |
| EcoT22I | ATGCAT | 2 cut(s) | 585, 1821 |
| EgeI | GGCGCC | 1 cut(s) | 1090 |
| EheI | GGCGCC | 1 cut(s) | 1090 |
| ErhI | CCWWGG | 5 cut(s) | 681, 1126, 1272, 1432, 1962 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 198, 230, 1076, 1108, 1960, 1992 |
| FaqI | GGGAC | 2 cut(s) | 989, 1354 |
| FblI | GTMKAC | 2 cut(s) | 522, 663 |
| Fnu4HI | GCNGC | 6 cut(s) | 210, 1163, 1219, 1242, 1501, 1833 |
| FokI | GGATG | 4 cut(s) | 251, 509, 869, 1751 |
| Fsp4HI | GCNGC | 6 cut(s) | 210, 1163, 1219, 1242, 1501, 1833 |
| FspBI | CTAG | 3 cut(s) | 531, 1001, 1808 |
| GlaI | GCGC | 4 cut(s) | 155, 1040, 1090, 1836 |
| GluI | GCNGC | 6 cut(s) | 210, 1163, 1219, 1242, 1501, 1833 |
| GsuI | CTGGAG | 1 cut(s) | 1592 |
| HaeII | RGCGCY | 3 cut(s) | 157, 1092, 1838 |
| HaeIII | GGCC | 7 cut(s) | 122, 674, 694, 1125, 1358, 1445, 2171 |
| HapII | CCGG | 5 cut(s) | 873, 994, 1007, 2116, 2186 |
| HhaI | GCGC | 4 cut(s) | 156, 1041, 1091, 1837 |
| Hin1I | GRCGYC | 1 cut(s) | 1089 |
| Hin6I | GCGC | 4 cut(s) | 154, 1039, 1089, 1835 |
| HinP1I | GCGC | 4 cut(s) | 154, 1039, 1089, 1835 |
| HincII | GTYRAC | 1 cut(s) | 523 |
| HindII | GTYRAC | 1 cut(s) | 523 |
| HindIII | AAGCTT | 1 cut(s) | 1172 |
| HpaII | CCGG | 5 cut(s) | 873, 994, 1007, 2116, 2186 |
| HphI | GGTGA | 6 cut(s) | 762, 805, 862, 1945, 2043, 2200 |
| Hpy166II | GTNNAC | 8 cut(s) | 250, 523, 664, 1024, 1026, 1486, 1945, 2208 |
| Hpy8I | GTNNAC | 8 cut(s) | 250, 523, 664, 1024, 1026, 1486, 1945, 2208 |
| Hpy99I | CGWCG | 2 cut(s) | 527, 2069 |
| HpyAV | CCTTC | 9 cut(s) | 334, 684, 1078, 1102, 1331, 1348, 2065, 2095, 2222 |
| HpyCH4III | ACNGT | 5 cut(s) | 844, 919, 1016, 1557, 1671 |
| HpyCH4IV | ACGT | 2 cut(s) | 275, 1941 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 1171, 1513, 1522, 2075, 2157 |
| HpyF3I | CTNAG | 9 cut(s) | 37, 215, 371, 717, 860, 1205, 1613, 1691, 2147 |
| HpySE526I | ACGT | 2 cut(s) | 275, 1941 |
| Hsp92I | GRCGYC | 1 cut(s) | 1089 |
| HspAI | GCGC | 4 cut(s) | 154, 1039, 1089, 1835 |
| KasI | GGCGCC | 1 cut(s) | 1088 |
| KspI | CCGCGG | 1 cut(s) | 2087 |
| Kzo9I | GATC | 3 cut(s) | 163, 343, 1399 |
| LmnI | GCTCC | 1 cut(s) | 1522 |
| Lsp1109I | GCAGC | 5 cut(s) | 221, 1174, 1205, 1228, 1512 |
| LweI | GCATC | 5 cut(s) | 21, 286, 553, 703, 1984 |
| MaeI | CTAG | 3 cut(s) | 531, 1001, 1808 |
| MaeII | ACGT | 2 cut(s) | 275, 1941 |
| MaeIII | GTNAC | 2 cut(s) | 280, 2188 |
| MalI | GATC | 3 cut(s) | 165, 345, 1401 |
| MboI | GATC | 3 cut(s) | 163, 343, 1399 |
| MflI | RGATCY | 1 cut(s) | 343 |
| MhlI | GDGCHC | 1 cut(s) | 1488 |
| MlsI | TGGCCA | 1 cut(s) | 1125 |
| MluNI | TGGCCA | 1 cut(s) | 1125 |
| Mly113I | GGCGCC | 1 cut(s) | 1089 |
| MlyI | GAGTC | 2 cut(s) | 35, 942 |
| MmeI | TCCRAC | 4 cut(s) | 309, 751, 1092, 2032 |
| Mox20I | TGGCCA | 1 cut(s) | 1125 |
| Mph1103I | ATGCAT | 2 cut(s) | 585, 1821 |
| MscI | TGGCCA | 1 cut(s) | 1125 |
| MseI | TTAA | 2 cut(s) | 1452, 1850 |
| MslI | CAYNNNNRTG | 3 cut(s) | 430, 1672, 2220 |
| Msp20I | TGGCCA | 1 cut(s) | 1125 |
| MspA1I | CMGCKG | 1 cut(s) | 2086 |
| MspCI | CTTAAG | 1 cut(s) | 1849 |
| MspI | CCGG | 5 cut(s) | 873, 994, 1007, 2116, 2186 |
| MspR9I | CCNGG | 2 cut(s) | 1007, 1075 |
| Mva1269I | GAATGC | 3 cut(s) | 1346, 1821, 1977 |
| MvaI | CCWGG | 1 cut(s) | 1075 |
| MvnI | CGCG | 3 cut(s) | 1039, 1832, 2086 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 1171, 1513, 1522, 2075, 2157 |
| NarI | GGCGCC | 1 cut(s) | 1089 |
| NciI | CCSGG | 1 cut(s) | 1007 |
| NcoI | CCATGG | 2 cut(s) | 681, 1962 |
| NdeII | GATC | 3 cut(s) | 163, 343, 1399 |
| NheI | GCTAGC | 1 cut(s) | 530 |
| NlaIV | GGNNCC | 4 cut(s) | 345, 1090, 1438, 1869 |
| NsiI | ATGCAT | 2 cut(s) | 585, 1821 |
| NspI | RCATGY | 4 cut(s) | 448, 931, 1055, 1690 |
| PagI | TCATGA | 2 cut(s) | 592, 1350 |
| PceI | AGGCCT | 3 cut(s) | 694, 1358, 1445 |
| PciI | ACATGT | 1 cut(s) | 927 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1479 |
| PctI | GAATGC | 3 cut(s) | 1346, 1821, 1977 |
| PflFI | GACNNNGTC | 1 cut(s) | 844 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1877 |
| PfoI | TCCNGGA | 1 cut(s) | 1005 |
| PkrI | GCNGC | 6 cut(s) | 211, 1164, 1220, 1243, 1502, 1834 |
| PleI | GAGTC | 2 cut(s) | 35, 942 |
| PluTI | GGCGCC | 1 cut(s) | 1092 |
| PpsI | GAGTC | 2 cut(s) | 35, 942 |
| PscI | ACATGT | 1 cut(s) | 927 |
| Psp6I | CCWGG | 1 cut(s) | 1073 |
| PspEI | GGTNACC | 1 cut(s) | 2188 |
| PspGI | CCWGG | 1 cut(s) | 1073 |
| PspN4I | GGNNCC | 4 cut(s) | 345, 1090, 1438, 1869 |
| PspPI | GGNCC | 3 cut(s) | 1009, 2081, 2199 |
| PsuI | RGATCY | 1 cut(s) | 343 |
| PsyI | GACNNNGTC | 1 cut(s) | 844 |
| RsaI | GTAC | 6 cut(s) | 249, 443, 1025, 1079, 1270, 1725 |
| RsaNI | GTAC | 6 cut(s) | 248, 442, 1024, 1078, 1269, 1724 |
| RseI | CAYNNNNRTG | 3 cut(s) | 430, 1672, 2220 |
| SacII | CCGCGG | 1 cut(s) | 2087 |
| SalI | GTCGAC | 1 cut(s) | 521 |
| SaqAI | TTAA | 2 cut(s) | 1452, 1850 |
| SatI | GCNGC | 6 cut(s) | 210, 1163, 1219, 1242, 1501, 1833 |
| Sau3AI | GATC | 3 cut(s) | 163, 343, 1399 |
| Sau96I | GGNCC | 3 cut(s) | 1009, 2081, 2199 |
| SchI | GAGTC | 2 cut(s) | 35, 942 |
| ScrFI | CCNGG | 2 cut(s) | 1007, 1075 |
| SduI | GDGCHC | 1 cut(s) | 1488 |
| SfaNI | GCATC | 5 cut(s) | 21, 286, 553, 703, 1984 |
| SfcI | CTRYAG | 1 cut(s) | 2194 |
| SfoI | GGCGCC | 1 cut(s) | 1090 |
| Sfr303I | CCGCGG | 1 cut(s) | 2087 |
| SgrBI | CCGCGG | 1 cut(s) | 2087 |
| SinI | GGWCC | 3 cut(s) | 1009, 2081, 2199 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 430, 1672, 2220 |
| SmlI | CTYRAG | 1 cut(s) | 1849 |
| SmoI | CTYRAG | 1 cut(s) | 1849 |
| SseBI | AGGCCT | 3 cut(s) | 694, 1358, 1445 |
| SsiI | CCGC | 5 cut(s) | 1507, 1832, 1865, 2084, 2086 |
| SspDI | GGCGCC | 1 cut(s) | 1088 |
| SspI | AATATT | 1 cut(s) | 700 |
| SspMI | CTAG | 3 cut(s) | 531, 1001, 1808 |
| StuI | AGGCCT | 3 cut(s) | 694, 1358, 1445 |
| StyD4I | CCNGG | 2 cut(s) | 1005, 1073 |
| StyI | CCWWGG | 5 cut(s) | 681, 1126, 1272, 1432, 1962 |
| TaaI | ACNGT | 5 cut(s) | 844, 919, 1016, 1557, 1671 |
| TaiI | ACGT | 2 cut(s) | 278, 1944 |
| TaqI | TCGA | 9 cut(s) | 134, 162, 323, 522, 1082, 1185, 1473, 1875, 2025 |
| TatI | WGTACW | 4 cut(s) | 247, 441, 1023, 1723 |
| TauI | GCSGC | 1 cut(s) | 1835 |
| Tru1I | TTAA | 2 cut(s) | 1452, 1850 |
| Tru9I | TTAA | 2 cut(s) | 1452, 1850 |
| TscAI | CASTG | 5 cut(s) | 922, 1021, 1383, 1663, 1674 |
| TseI | GCWGC | 5 cut(s) | 209, 1162, 1218, 1241, 1500 |
| TspGWI | ACGGA | 1 cut(s) | 1392 |
| TspRI | CASTG | 5 cut(s) | 922, 1021, 1383, 1663, 1674 |
| Tth111I | GACNNNGTC | 1 cut(s) | 844 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1877 |
| Vha464I | CTTAAG | 1 cut(s) | 1849 |
| VneI | GTGCAC | 1 cut(s) | 1484 |
| VpaK11BI | GGWCC | 3 cut(s) | 1009, 2081, 2199 |
| XapI | RAATTY | 6 cut(s) | 753, 779, 1630, 1778, 2034, 2060 |
| XbaI | TCTAGA | 1 cut(s) | 1807 |
| XceI | RCATGY | 4 cut(s) | 448, 931, 1055, 1690 |
| XmiI | GTMKAC | 2 cut(s) | 522, 663 |
| XspI | CTAG | 3 cut(s) | 531, 1001, 1808 |
| Zsp2I | ATGCAT | 2 cut(s) | 585, 1821 |