Rroxscaffold_4G00304080

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000330
Physical Location & Seq
Reverse (-)
24719076 .. 24732278
13203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00304080.1

Sequence Viewer

Length: 3093 bp
ATGGGTTTTCTCTATCTCCTCTTTATCTTCACACTTTCATCACTTTTCCTCTTCCTAGCCTTCGTCTCGTTTCTCATACTCAAACTCTACACAGGCAAGTCTATCAAAGACCCAAACTATCCACCTGTAAAAGGCACCGTCTTCCACCAGCTCTTATATTTCAACAAACTATACGACCACGAAGCCGAAATCGCCAGAGAAAACCCTACTTACCGGCTCCTCGCGGCGGACCACAGCGCTATATATACGTGCGACCCAAGAAACATCGAGCACGTGCTCAAAACCAGATTTGCTCACTACACGAAAGGGGAGTATAACCAGACTATTGTCAGTGATGTTTTTGGGCATGGGATTTTTGCTGTGGATGGAGATAAGTGGAGGCAACAGAGGAAGCTTGCAAGTTTCGAGTTTTCGACAAGGGTTTTGAGGGATTTTAGCTGTTCTGTCTTTAGAAGAAATGCTGCCAAAATTGTCAGAAAGGTTTCCGAGTTTTCCGAGTCCAGTGAGGTTTTTGATATGCAGGATTTGCTTATGAGATGTACCCTGGATTCCATATTCAAGGTTGGATTTGGAATTGAACTGAATTGCTTGGAGGGGTCAAGCAAAGAAGGGCTAGCATTCATGAAGGCATTTGATGAGTCAACTGCTCTGACCTATTGGCGCTATGTTGATCCATTCTGGAAATTGAAAAGATTTCTCAACAATGGTTCTGAAGCCACCCTTAGAAATTATGTCAAAGTTATTCATGACTTTGTGCACCAACTTATCAGGAGAAAGAGGACATTGCTAGCTAGCCAGAAAGATGGTAATGACAAGGAGGACATACTGTCACGGTTTCTATTGGAGAGTGAGAAGAATCCGGAGGACATGAATGATAAATATCTAAGTGACATAATTCTGAACTTCATGATTGCTGGGAAAGATACCAGTGCAAATACTCTCTCATGGTTCTTCTACATGCTCAGCAAGAACCCTCTAATACAGGAAACAGTCGCACAAGAAGTGAGGGATGTTGTTGGTCTGGATCATGAAGCCAACATTTACGAATTTGTGGCCAATATAACTGATGCAGCTCTTGAAAAAATGCACTATCTTCATGCGGCAATAACAGAGACCCTGAGGCTATACCCAGCAGTTCCTATTGATGGTAGAAGTGCAGAGGTAGATGACATTCTTCCTGATGGCTTTAGAGTGAAAAAAGGAGATAGTATTAACTACATGAGTTATGCCATGGGCAGAATGCCTTATATTTGGGGAAAGGATGCAGAGGAGTTTAGACCTGAACGATGGCTCAACAATGGAATTTTCCAGCCGGAATCACCTTTCAAATTTGTTGCATTTCATGCTGGTCCTCGGATCTGTCTGGGGAAGGACTTTGCTTATCGGCAGATGAAGATAGTAGCAATGGCTCTTCTTTGCTTCTTCCGCTTCAAATTGGCCGATGAAACAAAAAATGTAACCTATAGGACCATGTTCACCCTTCACATTGATGGAAGTCTTCCCTTGAGAAAGATGGTTATTCTCTATTTCCTCTTTGCTTTCACATGTCTGTTAGTTTTCCTCTTCATAGCTTTCTTGTCTTCCCTCATACTCAGAATCTTCACAGGCAAGTCCATCAGAGACCCAAGCTACCCGCCTGTAAAAGGCACCGTTTTTCACCAGCTCTTATACTTCACCAAACTCTATGACCACCAAACCCAAATAGCCAGAGAAACCCCAACTTACCGGCTTCTTTCTCCTAACCACAGCTCTGTATACACATGTGATGTGCGAAATATAGAGCATGTCCTCAAAACCAAATTTTCCACATATACGAAAGGTGCATATAATGAACTTATAATGGGTGACACTTTTGGTCATGGGATATTTGTTGTTGATGGAGAGAAGTGGAGGCAGCAGAGGAAGCTTGCAAGTATGGAGTTCTCGACCAGGATTCTTAGAGATTTTAGCTGCTCCGTGTTTAGAAGAAATGCAGCCAAACTGGTTAAAGTTGTTCATGAGTTTTCAGATTCCAATTGTTCTTTTGATATGCAGGACTTGCTTATGAGATGTACCTTGGATTCAATATTCAAAGTTGGGTTTGGAATAGAGCTGAATTGCCTGGAGGAGTCAAGCAAATCGGGGTTAGGATTTATGAAGGCTTTTGATGAGTCAACTGCTCTGACTTACCGGCGATATGTTGATCCATTCTGGAAAGTTAAAAGATTTCTCAACTTTGGTTCTGAGGCCAGCCTTAGAAAGTATGTCAAAGTCATTAATGATTTTGTGGACCAACTAATCAGGAGCAAGAGGAAATTGCTAGCTGGGGAGAAAGCTGGTAATGACAAGGAGGACATACTATCGAGGTTCCTTGTGGAAAGTGAGAAGGATCCGGAGCAAATGAATGACAAATATCTAAGTGATATAATTCTGAATTTTATGATTGCTGGCAAAGATACAAGTGCAAATACACTCTCATGGTTCTTCTACATGCTCAGCAAGAATCCTCTAATACAGGAGAAAGTTGCACAAGAAGTGATGGATGTCGTTAGTCTTAATCATGAAGCTAACATTGATGACTTTGTGGCAGATATAACTGATGCAGCTCTCGAACAAATGCATTATCTTCACGCTACACTAACTGAGACTTTGAGGCTATATCCTGCAGTTCCCCTGGATGGGAGATCTGCTGAGGTAGATGACATTCTTCCTGATGGCTTTAGAGTGAAAAAAGGAGATCGAATAACCTATATGGCCTATGCCATGGGAAGAATGCCTTACATATGGGGAAACGATGCTGAGGATTTCCGACCTGAAAGATGGCTCGAGAATGGAATTTTCAAGCCAGAGTCACCTTTCAAATTCGTTGCATTTCATGCAGGGCCTCGGATCTGTCTAGGGAAGGACTTTGCTTACCGACAGATGAAGATAGTTGCAATGGCTCTTCTCTACTTTTTCCGCTTCGAATTGGCTGACGAAACAAAGAACCACACCTCCATGATCTACTCTCCTTTTCCCTTGGCCTTCTCGCTCGTCCGACTGGGCATCCATGCCGAGCGGGCCTCTGGCTTTGTCGGTATGCACCTTGTGGTCCGACGGGAGTCACGGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

1030

Amino Acids

119.22

Weight (kDa)

8.16

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 55 - 494 2.6e-77 Cytochrome P450
p450 PF00067 550 - 993 9.3e-79 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44890 AT2G44890 AT2G45510
fragaria_vesca FvH4_7g12200 FvH4_7g12250 FvH4_7g12281 FvH4_7g12281 FvH4_7g12281 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12300 FvH4_7g12310
malus_domestica MD02G1200000.v1.1 MD02G1200100.v1.1 MD02G1200300.v1.1 MD02G1200400.v1.1 MD02G1200500.v1.1 MD02G1200600.v1.1 MD02G1200700.v1.1
prunus_persica Prupe.2G148400_v2.0.a1 Prupe.2G148500_v2.0.a1 Prupe.2G148600_v2.0.a1 Prupe.2G148700_v2.0.a1
pyrus_communis pycom02g16390
rosa_chinensis RchiOBHm_Chr1g0350941 RchiOBHm_Chr1g0350951 RchiOBHm_Chr1g0350981 RchiOBHm_Chr1g0350991 RchiOBHm_Chr1g0351001 RchiOBHm_Chr1g0351031 RchiOBHm_Chr1g0351061 RchiOBHm_Chr1g0351071 RchiOBHm_Chr1g0351081 RchiOBHm_Chr1g0351091 RchiOBHm_Chr1g0351101 RchiOBHm_Chr1g0375431 RchiOBHm_Chr2g0142281
rosa_laevigata RLG00000026651 RLG00000028432 RLG00000028433 RLG00000028435 RLG00000028436 RLG00000028437 RLG00000028438 RLG00000028439
rosa_multiflora Rmu_co7992102.1_g000001 Rmu_sc0000429.1_g000085 Rmu_sc0002665.1_g000014 Rmu_sc0002665.1_g000018 Rmu_sc0006087.1_g000001 Rmu_sc0006458.1_g000011 Rmu_sc0008688.1_g000002 Rmu_sc0008688.1_g000006 Rmu_sc0009646.1_g000012 Rmu_sc0032693.1_g000003
rosa_roxburghii Rroxscaffold_4G00304080 Rroxscaffold_4G00304120 Rroxscaffold_4G00304150 Rroxscaffold_4G00304170 Rroxscaffold_4G00304180 Rroxscaffold_4G00304190 Rroxscaffold_4G00304210 Rroxscaffold_4G00304230 Rroxscaffold_4G00304280
rosa_rugosa Rorug01G0075700 Rorug01G0215400 Rorug01G0216000 Rorug01G0216100 Rorug01G0216200 Rorug01G0216300 Rorug01G0216300 Rorug01G0216300 Rorug02G0238800 Rorug04G0006700 Rorug06G0023400
rosa_samantha Rh1AG230300 Rh1AG230400 Rh1AG230700 Rh1AG230800 Rh1BG200100 Rh1BG200200 Rh1BG200600 Rh1DG227900 Rh1DG228000 Rh1DG228200
rosa_wichuraiana Rw0G010880 Rw1G019960 Rw1G019970 Rw1G019990 Rw1G020000 Rw1G020010 Rw1G020040 Rw1G020050 Rw1G020060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1838
AccB1I GGYRCC 2 cut(s) 134, 1646
AccBSI CCGCTC 1 cut(s) 3037
AccI GTMKAC 1 cut(s) 1755
AccII CGCG 1 cut(s) 224
AccIII TCCGGA 2 cut(s) 859, 2371
AciI CCGC 7 cut(s) 224, 227, 1100, 1426, 1634, 2938, 3037
AclWI GGATC 7 cut(s) 665, 1032, 1364, 2177, 2363, 2376, 2876
AcoI YGGCCR 2 cut(s) 1053, 1437
AcsI RAATTY 7 cut(s) 1046, 1302, 1328, 1799, 2413, 2814, 2840
AcuI CTGAAG 1 cut(s) 732
AcvI CACGTG 1 cut(s) 274
AdeI CACNNNGTG 1 cut(s) 3067
AfaI GTAC 2 cut(s) 541, 2053
AfeI AGCGCT 1 cut(s) 238
AfiI CCNNNNNNNGG 6 cut(s) 131, 226, 1145, 1643, 2657, 2658
AflIII ACRYGT 2 cut(s) 1544, 1760
AhdI GACNNNNNGTC 1 cut(s) 826
AjnI CCWGG 4 cut(s) 543, 1928, 2100, 2652
AloI GAACNNNNNNTCC 2 cut(s) 2957, 2989
Alw21I GWGCWC 3 cut(s) 273, 279, 759
Alw26I GTCTC 4 cut(s) 70, 1106, 1614, 2618
Alw44I GTGCAC 1 cut(s) 755
AlwI GGATC 7 cut(s) 665, 1032, 1364, 2177, 2363, 2376, 2876
Ama87I CYCGRG 1 cut(s) 2804
Aor13HI TCCGGA 2 cut(s) 859, 2371
Aor51HI AGCGCT 1 cut(s) 238
AoxI GGCC 7 cut(s) 1053, 1437, 2226, 2733, 2861, 3000, 3039
ApaLI GTGCAC 1 cut(s) 755
ApeKI GCWGC 6 cut(s) 461, 1070, 1894, 1950, 1973, 2582
ApoI RAATTY 7 cut(s) 1046, 1302, 1328, 1799, 2413, 2814, 2840
ArsI GACNNNNNNTTYG 2 cut(s) 406, 438
AseI ATTAAT 1 cut(s) 2256
Asp700I GAANNNNTTC 1 cut(s) 481
AspLEI GCGC 2 cut(s) 239, 663
AspS9I GGNCC 7 cut(s) 229, 1349, 1467, 2269, 2861, 3039, 3070
AsuHPI GGTGA 6 cut(s) 1311, 1468, 1649, 1666, 1856, 2823
AsuII TTCGAA 1 cut(s) 2943
AsuNHI GCTAGC 4 cut(s) 613, 787, 791, 2299
AvaI CYCGRG 1 cut(s) 2804
AvaII GGWCC 5 cut(s) 229, 1349, 1467, 2269, 3070
AxyI CCTNAGG 1 cut(s) 1118
BaeGI GKGCMC 1 cut(s) 759
BalI TGGCCA 1 cut(s) 1055
BamHI GGATCC 1 cut(s) 2368
BanI GGYRCC 2 cut(s) 134, 1646
BbrPI CACGTG 1 cut(s) 274
BbsI GAAGAC 3 cut(s) 133, 1490, 1572
Bbv12I GWGCWC 3 cut(s) 273, 279, 759
BbvCI CCTCAGC 2 cut(s) 2670, 2778
BbvI GCAGC 6 cut(s) 448, 1082, 1906, 1937, 1985, 2594
BciT130I CCWGG 4 cut(s) 545, 1930, 2102, 2654
BcoDI GTCTC 4 cut(s) 70, 1106, 1614, 2618
BfaI CTAG 7 cut(s) 56, 614, 788, 792, 2300, 2876, 3091
BfmI CTRYAG 2 cut(s) 1462, 2643
BfoI RGCGCY 2 cut(s) 240, 664
BglI GCCNNNNNGGC 1 cut(s) 3038
BglII AGATCT 1 cut(s) 2663
BisI GCNGC 8 cut(s) 225, 462, 1071, 1101, 1895, 1951, 1974, 2583
BlpI GCTNAGC 2 cut(s) 962, 2474
BlsI GCNGC 8 cut(s) 226, 463, 1072, 1102, 1896, 1952, 1975, 2584
Bme1390I CCNGG 4 cut(s) 545, 1930, 2102, 2654
Bme18I GGWCC 5 cut(s) 229, 1349, 1467, 2269, 3070
BmeRI GACNNNNNGTC 1 cut(s) 826
BmeT110I CYCGRG 1 cut(s) 2804
BmgT120I GGNCC 7 cut(s) 229, 1349, 1467, 2269, 2861, 3039, 3070
BmiI GGNNCC 5 cut(s) 136, 218, 1648, 2348, 2370
BmrFI CCNGG 4 cut(s) 545, 1930, 2102, 2654
BmrI ACTGGG 1 cut(s) 3029
BmsI GCATC 5 cut(s) 1057, 1252, 2569, 2764, 3033
BmtI GCTAGC 4 cut(s) 617, 791, 795, 2303
BmuI ACTGGG 1 cut(s) 3029
BoxI GACNNNNGTC 2 cut(s) 326, 3079
BpiI GAAGAC 3 cut(s) 133, 1490, 1572
BplI GAGNNNNNCTC 2 cut(s) 3026, 3058
BpmI CTGGAG 1 cut(s) 2123
Bpu10I CCTNAGC 2 cut(s) 2670, 2778
Bpu1102I GCTNAGC 2 cut(s) 962, 2474
Bpu14I TTCGAA 1 cut(s) 2943
BpuEI CTTGAG 1 cut(s) 1525
BsaAI YACGTR 2 cut(s) 249, 274
BsaBI GATNNNNATC 1 cut(s) 879
BsaI GGTCTC 2 cut(s) 1106, 1614
BsaJI CCNNGG 8 cut(s) 543, 1230, 1352, 2055, 2652, 2742, 2864, 2997
BsaWI WCCGGW 2 cut(s) 859, 2371
BsaXI ACNNNNNCTCC 2 cut(s) 2957, 2987
Bsc4I CCNNNNNNNGG 6 cut(s) 131, 226, 1145, 1643, 2657, 2658
Bse118I RCCGGY 3 cut(s) 213, 1725, 2169
Bse1I ACTGG 4 cut(s) 501, 927, 1986, 3024
Bse21I CCTNAGG 1 cut(s) 1118
Bse3DI GCAATG 3 cut(s) 782, 1410, 2922
Bse8I GATNNNNATC 1 cut(s) 879
BseAI TCCGGA 2 cut(s) 859, 2371
BseBI CCWGG 4 cut(s) 545, 1930, 2102, 2654
BseDI CCNNGG 8 cut(s) 543, 1230, 1352, 2055, 2652, 2742, 2864, 2997
BseGI GGATG 6 cut(s) 370, 1015, 1267, 2527, 2662, 3024
BseJI GATNNNNATC 1 cut(s) 879
BseLI CCNNNNNNNGG 6 cut(s) 131, 226, 1145, 1643, 2657, 2658
BseMI GCAATG 3 cut(s) 782, 1410, 2922
BseMII CTCAG 8 cut(s) 976, 1109, 1606, 2214, 2488, 2613, 2661, 2769
BseNI ACTGG 4 cut(s) 501, 927, 1986, 3024
BseRI GAGGAG 4 cut(s) 8, 209, 1283, 2120
BseSI GKGCMC 1 cut(s) 759
BseXI GCAGC 6 cut(s) 448, 1082, 1906, 1937, 1985, 2594
BseYI CCCAGC 3 cut(s) 914, 1129, 2303
BsgI GTGCAG 1 cut(s) 1176
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 7 cut(s) 1055, 1439, 2228, 2735, 2863, 3002, 3041
BshNI GGYRCC 2 cut(s) 134, 1646
BsiHKAI GWGCWC 3 cut(s) 273, 279, 759
BsiHKCI CYCGRG 1 cut(s) 2804
BsiSI CCGG 6 cut(s) 214, 860, 1313, 1726, 2170, 2372
BslI CCNNNNNNNGG 6 cut(s) 131, 226, 1145, 1643, 2657, 2658
BsmAI GTCTC 4 cut(s) 70, 1106, 1614, 2618
BsmBI CGTCTC 1 cut(s) 70
BsmI GAATGC 3 cut(s) 617, 1245, 2757
BsnI GGCC 7 cut(s) 1055, 1439, 2228, 2735, 2863, 3002, 3041
Bso31I GGTCTC 2 cut(s) 1106, 1614
BsoBI CYCGRG 1 cut(s) 2804
Bsp119I TTCGAA 1 cut(s) 2943
Bsp1286I GDGCHC 3 cut(s) 273, 279, 759
Bsp13I TCCGGA 2 cut(s) 859, 2371
Bsp143I GATC 9 cut(s) 670, 1024, 1356, 2182, 2368, 2663, 2716, 2868, 2979
Bsp1720I GCTNAGC 2 cut(s) 962, 2474
Bsp19I CCATGG 2 cut(s) 1230, 2742
BspACI CCGC 7 cut(s) 224, 227, 1100, 1426, 1634, 2938, 3037
BspANI GGCC 7 cut(s) 1055, 1439, 2228, 2735, 2863, 3002, 3041
BspCNI CTCAG 8 cut(s) 975, 1110, 1605, 2215, 2487, 2614, 2662, 2770
BspEI TCCGGA 2 cut(s) 859, 2371
BspFNI CGCG 1 cut(s) 224
BspHI TCATGA 6 cut(s) 621, 745, 906, 1027, 1996, 2539
BspLI GGNNCC 5 cut(s) 136, 218, 1648, 2348, 2370
BspMAI CTGCAG 1 cut(s) 2647
BspOI GCTAGC 4 cut(s) 617, 791, 795, 2303
BspPI GGATC 7 cut(s) 665, 1032, 1364, 2177, 2363, 2376, 2876
BspQI GCTCTTC 2 cut(s) 1416, 2928
BspT104I TTCGAA 1 cut(s) 2943
BspT107I GGYRCC 2 cut(s) 134, 1646
BspTNI GGTCTC 2 cut(s) 1106, 1614
BsrBI CCGCTC 1 cut(s) 3037
BsrDI GCAATG 3 cut(s) 782, 1410, 2922
BsrFI RCCGGY 3 cut(s) 213, 1725, 2169
BsrI ACTGG 4 cut(s) 501, 927, 1986, 3024
BssAI RCCGGY 3 cut(s) 213, 1725, 2169
BssECI CCNNGG 8 cut(s) 543, 1230, 1352, 2055, 2652, 2742, 2864, 2997
BssMI GATC 9 cut(s) 670, 1024, 1356, 2182, 2368, 2663, 2716, 2868, 2979
BssNAI GTATAC 1 cut(s) 1756
BssT1I CCWWGG 4 cut(s) 1230, 2055, 2742, 2997
Bst1107I GTATAC 1 cut(s) 1756
Bst2UI CCWGG 4 cut(s) 545, 1930, 2102, 2654
Bst4CI ACNGT 5 cut(s) 139, 828, 834, 991, 1651
Bst6I CTCTTC 4 cut(s) 56, 1416, 1568, 2928
BstAPI GCANNNNNTGC 4 cut(s) 526, 1343, 2038, 2855
BstBAI YACGTR 2 cut(s) 249, 274
BstBI TTCGAA 1 cut(s) 2943
BstC8I GCNNGC 9 cut(s) 396, 615, 789, 793, 1908, 2230, 2301, 2428, 3039
BstDSI CCRYGG 2 cut(s) 1230, 2742
BstENI CCTNNNNNAGG 2 cut(s) 129, 1641
BstF5I GGATG 6 cut(s) 370, 1015, 1267, 2527, 2662, 3024
BstFNI CGCG 1 cut(s) 224
BstH2I RGCGCY 2 cut(s) 240, 664
BstHHI GCGC 2 cut(s) 239, 663
BstKTI GATC 9 cut(s) 673, 1027, 1359, 2185, 2371, 2666, 2719, 2871, 2982
BstMAI GTCTC 4 cut(s) 70, 1106, 1614, 2618
BstMBI GATC 9 cut(s) 670, 1024, 1356, 2182, 2368, 2663, 2716, 2868, 2979
BstMWI GCNNNNNNNGC 8 cut(s) 191, 526, 1343, 1425, 1903, 2038, 2855, 3038
BstNI CCWGG 4 cut(s) 545, 1930, 2102, 2654
BstNSI RCATGY 5 cut(s) 961, 1548, 1764, 1787, 2473
BstPAI GACNNNNGTC 2 cut(s) 326, 3079
BstSCI CCNGG 4 cut(s) 543, 1928, 2100, 2652
BstSFI CTRYAG 2 cut(s) 1462, 2643
BstSLI GKGCMC 1 cut(s) 759
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 6 cut(s) 448, 1082, 1906, 1937, 1985, 2594
BstV2I GAAGAC 3 cut(s) 133, 1490, 1572
BstX2I RGATCY 4 cut(s) 1356, 2368, 2663, 2868
BstXI CCANNNNNNTGG 1 cut(s) 803
BstYI RGATCY 4 cut(s) 1356, 2368, 2663, 2868
BstZ17I GTATAC 1 cut(s) 1756
Bsu36I CCTNAGG 1 cut(s) 1118
BsuRI GGCC 7 cut(s) 1055, 1439, 2228, 2735, 2863, 3002, 3041
BtgI CCRYGG 2 cut(s) 1230, 2742
BtsCI GGATG 6 cut(s) 370, 1015, 1267, 2527, 2662, 3024
BtsIMutI CAGTG 3 cut(s) 337, 508, 934
Cac8I GCNNGC 9 cut(s) 396, 615, 789, 793, 1908, 2230, 2301, 2428, 3039
CciI TCATGA 6 cut(s) 621, 745, 906, 1027, 1996, 2539
CfoI GCGC 2 cut(s) 239, 663
Cfr10I RCCGGY 3 cut(s) 213, 1725, 2169
Cfr13I GGNCC 7 cut(s) 229, 1349, 1467, 2269, 2861, 3039, 3070
Csp6I GTAC 2 cut(s) 540, 2052
CviQI GTAC 2 cut(s) 540, 2052
DpnI GATC 9 cut(s) 672, 1026, 1358, 2184, 2370, 2665, 2718, 2870, 2981
DpnII GATC 9 cut(s) 670, 1024, 1356, 2182, 2368, 2663, 2716, 2868, 2979
DraIII CACNNNGTG 1 cut(s) 3067
DriI GACNNNNNGTC 1 cut(s) 826
EaeI YGGCCR 2 cut(s) 1053, 1437
Eam1104I CTCTTC 4 cut(s) 56, 1416, 1568, 2928
Eam1105I GACNNNNNGTC 1 cut(s) 826
EarI CTCTTC 4 cut(s) 56, 1416, 1568, 2928
EciI GGCGGA 1 cut(s) 242
Eco130I CCWWGG 4 cut(s) 1230, 2055, 2742, 2997
Eco31I GGTCTC 2 cut(s) 1106, 1614
Eco47I GGWCC 5 cut(s) 229, 1349, 1467, 2269, 3070
Eco47III AGCGCT 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 732
Eco72I CACGTG 1 cut(s) 274
Eco81I CCTNAGG 1 cut(s) 1118
Eco88I CYCGRG 1 cut(s) 2804
EcoNI CCTNNNNNAGG 2 cut(s) 129, 1641
EcoO109I RGGNCCY 1 cut(s) 2861
EcoRII CCWGG 4 cut(s) 543, 1928, 2100, 2652
EcoT14I CCWWGG 4 cut(s) 1230, 2055, 2742, 2997
EcoT22I ATGCAT 1 cut(s) 2601
ErhI CCWWGG 4 cut(s) 1230, 2055, 2742, 2997
Esp3I CGTCTC 1 cut(s) 70
FalI AAGNNNNNCTT 4 cut(s) 705, 737, 2740, 2772
FauI CCCGC 2 cut(s) 1641, 3030
FauNDI CATATG 1 cut(s) 2762
FblI GTMKAC 1 cut(s) 1755
Fnu4HI GCNGC 8 cut(s) 225, 462, 1071, 1101, 1895, 1951, 1974, 2583
FokI GGATG 6 cut(s) 377, 1022, 1274, 2534, 2669, 3011
Fsp4HI GCNGC 8 cut(s) 225, 462, 1071, 1101, 1895, 1951, 1974, 2583
FspBI CTAG 7 cut(s) 56, 614, 788, 792, 2300, 2876, 3091
GlaI GCGC 2 cut(s) 238, 662
GluI GCNGC 8 cut(s) 225, 462, 1071, 1101, 1895, 1951, 1974, 2583
GsaI CCCAGC 3 cut(s) 918, 1133, 2307
GsuI CTGGAG 1 cut(s) 2123
HaeII RGCGCY 2 cut(s) 240, 664
HaeIII GGCC 7 cut(s) 1055, 1439, 2228, 2735, 2863, 3002, 3041
HapII CCGG 6 cut(s) 214, 860, 1313, 1726, 2170, 2372
HhaI GCGC 2 cut(s) 239, 663
Hin6I GCGC 2 cut(s) 237, 661
HinP1I GCGC 2 cut(s) 237, 661
HincII GTYRAC 2 cut(s) 642, 2154
HindII GTYRAC 2 cut(s) 642, 2154
HindIII AAGCTT 2 cut(s) 392, 1904
HpaII CCGG 6 cut(s) 214, 860, 1313, 1726, 2170, 2372
HphI GGTGA 6 cut(s) 1311, 1468, 1649, 1666, 1856, 2823
Hpy166II GTNNAC 6 cut(s) 642, 757, 1476, 1756, 2154, 2269
Hpy8I GTNNAC 6 cut(s) 642, 757, 1476, 1756, 2154, 2269
Hpy99I CGWCG 1 cut(s) 3078
HpyAV CCTTC 9 cut(s) 70, 602, 619, 1363, 1490, 2131, 2359, 2875, 3013
HpyCH4III ACNGT 5 cut(s) 139, 828, 834, 991, 1651
HpyCH4IV ACGT 2 cut(s) 248, 273
HpyF10VI GCNNNNNNNGC 8 cut(s) 191, 526, 1343, 1425, 1903, 2038, 2855, 3038
HpySE526I ACGT 2 cut(s) 248, 273
HspAI GCGC 2 cut(s) 237, 661
Kpn2I TCCGGA 2 cut(s) 859, 2371
Kzo9I GATC 9 cut(s) 670, 1024, 1356, 2182, 2368, 2663, 2716, 2868, 2979
LguI GCTCTTC 2 cut(s) 1416, 2928
LmnI GCTCC 4 cut(s) 222, 1958, 2283, 2374
Lsp1109I GCAGC 6 cut(s) 448, 1082, 1906, 1937, 1985, 2594
LweI GCATC 5 cut(s) 1057, 1252, 2569, 2764, 3033
MaeI CTAG 7 cut(s) 56, 614, 788, 792, 2300, 2876, 3091
MaeII ACGT 2 cut(s) 248, 273
MaeIII GTNAC 6 cut(s) 828, 887, 1456, 1844, 2829, 3081
MalI GATC 9 cut(s) 672, 1026, 1358, 2184, 2370, 2665, 2718, 2870, 2981
MbiI CCGCTC 1 cut(s) 3037
MboI GATC 9 cut(s) 670, 1024, 1356, 2182, 2368, 2663, 2716, 2868, 2979
MfeI CAATTG 1 cut(s) 2014
MflI RGATCY 4 cut(s) 1356, 2368, 2663, 2868
MhlI GDGCHC 3 cut(s) 273, 279, 759
MlsI TGGCCA 1 cut(s) 1055
MluNI TGGCCA 1 cut(s) 1055
MlyI GAGTC 6 cut(s) 506, 647, 2117, 2159, 2837, 3089
MmeI TCCRAC 3 cut(s) 544, 2812, 3040
Mox20I TGGCCA 1 cut(s) 1055
Mph1103I ATGCAT 1 cut(s) 2601
MroI TCCGGA 2 cut(s) 859, 2371
MroXI GAANNNNTTC 1 cut(s) 481
MscI TGGCCA 1 cut(s) 1055
MseI TTAA 5 cut(s) 1212, 1986, 2199, 2256, 2535
MslI CAYNNNNRTG 3 cut(s) 1488, 2455, 2975
Msp20I TGGCCA 1 cut(s) 1055
MspI CCGG 6 cut(s) 214, 860, 1313, 1726, 2170, 2372
MspR9I CCNGG 4 cut(s) 545, 1930, 2102, 2654
MunI CAATTG 1 cut(s) 2014
Mva1269I GAATGC 3 cut(s) 617, 1245, 2757
MvaI CCWGG 4 cut(s) 545, 1930, 2102, 2654
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 8 cut(s) 191, 526, 1343, 1425, 1903, 2038, 2855, 3038
NcoI CCATGG 2 cut(s) 1230, 2742
NdeI CATATG 1 cut(s) 2762
NdeII GATC 9 cut(s) 670, 1024, 1356, 2182, 2368, 2663, 2716, 2868, 2979
NheI GCTAGC 4 cut(s) 613, 787, 791, 2299
NlaIV GGNNCC 5 cut(s) 136, 218, 1648, 2348, 2370
NmeAIII GCCGAG 1 cut(s) 3058
NmuCI GTSAC 5 cut(s) 828, 887, 1844, 2829, 3081
NsiI ATGCAT 1 cut(s) 2601
NspI RCATGY 5 cut(s) 961, 1548, 1764, 1787, 2473
NspV TTCGAA 1 cut(s) 2943
PaeR7I CTCGAG 1 cut(s) 2804
PagI TCATGA 6 cut(s) 621, 745, 906, 1027, 1996, 2539
PciI ACATGT 2 cut(s) 1544, 1760
PciSI GCTCTTC 2 cut(s) 1416, 2928
PctI GAATGC 3 cut(s) 617, 1245, 2757
PdmI GAANNNNTTC 1 cut(s) 481
PfeI GAWTC 8 cut(s) 548, 856, 1316, 1596, 1933, 2009, 2060, 2482
PkrI GCNGC 8 cut(s) 226, 463, 1072, 1102, 1896, 1952, 1975, 2584
PleI GAGTC 6 cut(s) 505, 646, 2116, 2158, 2836, 3088
PmaCI CACGTG 1 cut(s) 274
PmlI CACGTG 1 cut(s) 274
PpsI GAGTC 6 cut(s) 505, 646, 2116, 2158, 2836, 3088
Ppu21I YACGTR 2 cut(s) 249, 274
PscI ACATGT 2 cut(s) 1544, 1760
PshAI GACNNNNGTC 2 cut(s) 326, 3079
PshBI ATTAAT 1 cut(s) 2256
PsiI TTATAA 1 cut(s) 1838
Psp6I CCWGG 4 cut(s) 543, 1928, 2100, 2652
PspCI CACGTG 1 cut(s) 274
PspFI CCCAGC 3 cut(s) 914, 1129, 2303
PspGI CCWGG 4 cut(s) 543, 1928, 2100, 2652
PspN4I GGNNCC 5 cut(s) 136, 218, 1648, 2348, 2370
PspPI GGNCC 7 cut(s) 229, 1349, 1467, 2269, 2861, 3039, 3070
PstI CTGCAG 1 cut(s) 2647
PsuI RGATCY 4 cut(s) 1356, 2368, 2663, 2868
RsaI GTAC 2 cut(s) 541, 2053
RsaNI GTAC 2 cut(s) 540, 2052
RseI CAYNNNNRTG 3 cut(s) 1488, 2455, 2975
SapI GCTCTTC 2 cut(s) 1416, 2928
SaqAI TTAA 5 cut(s) 1212, 1986, 2199, 2256, 2535
SatI GCNGC 8 cut(s) 225, 462, 1071, 1101, 1895, 1951, 1974, 2583
Sau3AI GATC 9 cut(s) 670, 1024, 1356, 2182, 2368, 2663, 2716, 2868, 2979
Sau96I GGNCC 7 cut(s) 229, 1349, 1467, 2269, 2861, 3039, 3070
SchI GAGTC 6 cut(s) 506, 647, 2117, 2159, 2837, 3089
ScrFI CCNGG 4 cut(s) 545, 1930, 2102, 2654
SduI GDGCHC 3 cut(s) 273, 279, 759
SfaNI GCATC 5 cut(s) 1057, 1252, 2569, 2764, 3033
SfcI CTRYAG 2 cut(s) 1462, 2643
Sfr274I CTCGAG 1 cut(s) 2804
SfuI TTCGAA 1 cut(s) 2943
SinI GGWCC 5 cut(s) 229, 1349, 1467, 2269, 3070
SlaI CTCGAG 1 cut(s) 2804
SmiMI CAYNNNNRTG 3 cut(s) 1488, 2455, 2975
SmlI CTYRAG 2 cut(s) 1504, 2804
SmoI CTYRAG 2 cut(s) 1504, 2804
SsiI CCGC 7 cut(s) 224, 227, 1100, 1426, 1634, 2938, 3037
SspI AATATT 1 cut(s) 2067
SspMI CTAG 7 cut(s) 56, 614, 788, 792, 2300, 2876, 3091
StyD4I CCNGG 4 cut(s) 543, 1928, 2100, 2652
StyI CCWWGG 4 cut(s) 1230, 2055, 2742, 2997
TaaI ACNGT 5 cut(s) 139, 828, 834, 991, 1651
TaiI ACGT 2 cut(s) 251, 276
TaqI TCGA 9 cut(s) 267, 405, 413, 1925, 2342, 2589, 2719, 2805, 2943
TauI GCSGC 2 cut(s) 227, 1103
TfiI GAWTC 8 cut(s) 548, 856, 1316, 1596, 1933, 2009, 2060, 2482
Tru1I TTAA 5 cut(s) 1212, 1986, 2199, 2256, 2535
Tru9I TTAA 5 cut(s) 1212, 1986, 2199, 2256, 2535
TscAI CASTG 3 cut(s) 337, 508, 934
TseFI GTSAC 5 cut(s) 828, 887, 1844, 2829, 3081
TseI GCWGC 6 cut(s) 461, 1070, 1894, 1950, 1973, 2582
Tsp45I GTSAC 5 cut(s) 828, 887, 1844, 2829, 3081
TspGWI ACGGA 1 cut(s) 1945
TspRI CASTG 3 cut(s) 337, 508, 934
VneI GTGCAC 1 cut(s) 755
VpaK11BI GGWCC 5 cut(s) 229, 1349, 1467, 2269, 3070
VspI ATTAAT 1 cut(s) 2256
XagI CCTNNNNNAGG 2 cut(s) 129, 1641
XapI RAATTY 7 cut(s) 1046, 1302, 1328, 1799, 2413, 2814, 2840
XceI RCATGY 5 cut(s) 961, 1548, 1764, 1787, 2473
XhoI CTCGAG 1 cut(s) 2804
XmiI GTMKAC 1 cut(s) 1755
XmnI GAANNNNTTC 1 cut(s) 481
XspI CTAG 7 cut(s) 56, 614, 788, 792, 2300, 2876, 3091
Zsp2I ATGCAT 1 cut(s) 2601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.