MD02G1200100.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000330
Physical Location & Seq
Reverse (-)
19691050 .. 19692332
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1200100.v1.1.491

Sequence Viewer

Length: 711 bp
ATGGGGTTTTTCCCTATCATCTTCACCTCCATACTTATGTTTCTCTTCCTAACTGTGTCTTTGTTCATACTCAAAATCTTCACCGGAAAGTCTATCCGAGACCCGAATTACCCTCCCGTAAAAGGCACTGTCTTCCACATGTTCTTATACTTCAACAGCGTCTACAACTACCAAACTGTTGTTGCCAAAACACACTCAACTTTCCAGCTTCTAGTCCTGGACCGCAGCGAAGTGTACACGGCTGACACGCGCAACATTGAGCATGTTCTGAAAACCAACTTTGCCAGCTACTCGAAAGGCGCCTTCACTCAAAGTATTTTGTCGGATATTTTTGGCCAAGGGATATTTGTTGTTGATGGAGAAAAATGGAAGCAGCAGAGGAAGCTTGCGAGCTTCGAGTTCTCAACAAGGGTGCTTAGAGATGCTAGCTGCTCTGTGTTTAGAAGAAGTGTTGCCAAACTGGTTAGAGTTATTTTTGGAATTTCGAGTTCCGGAGGGGTTTTTGATATGCAGGATTTGCTTATGAGATGTACCTTGGATTCCATATTCAAAGTCGGGTTTGGAATAGAACTGCATTGCCTGGAGGGGTCAGGCAAAGAAGGGACTGCATTCATGAAGGCCTTTGATGAATCTACTGCACTGACCTATTTTCGCTACATTGATCCATTCTGGAAACTAAAAAGAATCTTCAACCTTGGTTCAAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

237

Amino Acids

26.96

Weight (kDa)

9.49

Isoelectric Point (pI)

30.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 39 - 228 1.8e-14 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44890 AT2G44890 AT2G45510
fragaria_vesca FvH4_7g12200 FvH4_7g12250 FvH4_7g12281 FvH4_7g12281 FvH4_7g12281 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12282 FvH4_7g12300 FvH4_7g12310
malus_domestica MD02G1200000.v1.1 MD02G1200100.v1.1 MD02G1200300.v1.1 MD02G1200400.v1.1 MD02G1200500.v1.1 MD02G1200600.v1.1 MD02G1200700.v1.1
prunus_persica Prupe.2G148400_v2.0.a1 Prupe.2G148500_v2.0.a1 Prupe.2G148600_v2.0.a1 Prupe.2G148700_v2.0.a1
pyrus_communis pycom02g16390
rosa_chinensis RchiOBHm_Chr1g0350941 RchiOBHm_Chr1g0350951 RchiOBHm_Chr1g0350981 RchiOBHm_Chr1g0350991 RchiOBHm_Chr1g0351001 RchiOBHm_Chr1g0351031 RchiOBHm_Chr1g0351061 RchiOBHm_Chr1g0351071 RchiOBHm_Chr1g0351081 RchiOBHm_Chr1g0351091 RchiOBHm_Chr1g0351101 RchiOBHm_Chr1g0375431 RchiOBHm_Chr2g0142281
rosa_laevigata RLG00000026651 RLG00000028432 RLG00000028433 RLG00000028435 RLG00000028436 RLG00000028437 RLG00000028438 RLG00000028439
rosa_multiflora Rmu_co7992102.1_g000001 Rmu_sc0000429.1_g000085 Rmu_sc0002665.1_g000014 Rmu_sc0002665.1_g000018 Rmu_sc0006087.1_g000001 Rmu_sc0006458.1_g000011 Rmu_sc0008688.1_g000002 Rmu_sc0008688.1_g000006 Rmu_sc0009646.1_g000012 Rmu_sc0032693.1_g000003
rosa_roxburghii Rroxscaffold_4G00304080 Rroxscaffold_4G00304120 Rroxscaffold_4G00304150 Rroxscaffold_4G00304170 Rroxscaffold_4G00304180 Rroxscaffold_4G00304190 Rroxscaffold_4G00304210 Rroxscaffold_4G00304230 Rroxscaffold_4G00304280
rosa_rugosa Rorug01G0075700 Rorug01G0215400 Rorug01G0216000 Rorug01G0216100 Rorug01G0216200 Rorug01G0216300 Rorug01G0216300 Rorug01G0216300 Rorug02G0238800 Rorug04G0006700 Rorug06G0023400
rosa_samantha Rh1AG230300 Rh1AG230400 Rh1AG230700 Rh1AG230800 Rh1BG200100 Rh1BG200200 Rh1BG200600 Rh1DG227900 Rh1DG228000 Rh1DG228200
rosa_wichuraiana Rw0G010880 Rw1G019960 Rw1G019970 Rw1G019990 Rw1G020000 Rw1G020010 Rw1G020040 Rw1G020050 Rw1G020060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 162
AccII CGCG 1 cut(s) 250
AccIII TCCGGA 1 cut(s) 491
AciI CCGC 1 cut(s) 223
AclWI GGATC 1 cut(s) 656
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 1 cut(s) 480
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 2 cut(s) 236, 532
AfiI CCNNNNNNNGG 1 cut(s) 122
AflIII ACRYGT 1 cut(s) 138
AgsI TTSAA 4 cut(s) 154, 550, 691, 702
AjnI CCWGG 2 cut(s) 216, 579
AluBI AGCT 5 cut(s) 208, 288, 385, 393, 429
AluI AGCT 5 cut(s) 208, 288, 385, 393, 429
Alw26I GTCTC 1 cut(s) 93
AlwI GGATC 1 cut(s) 656
Aor13HI TCCGGA 1 cut(s) 491
AoxI GGCC 2 cut(s) 334, 618
ApeKI GCWGC 3 cut(s) 225, 373, 429
ApoI RAATTY 1 cut(s) 480
AspLEI GCGC 2 cut(s) 252, 302
AspS9I GGNCC 1 cut(s) 220
AsuHPI GGTGA 2 cut(s) 16, 73
AsuNHI GCTAGC 1 cut(s) 425
AvaII GGWCC 1 cut(s) 220
BalI TGGCCA 1 cut(s) 336
BanI GGYRCC 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 124
BbvI GCAGC 3 cut(s) 237, 385, 416
BccI CCATC 1 cut(s) 350
BceAI ACGGC 1 cut(s) 255
BciT130I CCWGG 2 cut(s) 218, 581
BcoDI GTCTC 1 cut(s) 93
BfaI CTAG 2 cut(s) 212, 426
BfoI RGCGCY 1 cut(s) 303
BisI GCNGC 3 cut(s) 226, 374, 430
BlsI GCNGC 3 cut(s) 227, 375, 431
Bme1390I CCNGG 2 cut(s) 218, 581
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 1 cut(s) 301
BmrFI CCNGG 2 cut(s) 218, 581
BmsI GCATC 1 cut(s) 412
BmtI GCTAGC 1 cut(s) 429
BpiI GAAGAC 1 cut(s) 124
BpmI CTGGAG 1 cut(s) 602
BsaHI GRCGYC 1 cut(s) 300
BsaI GGTCTC 1 cut(s) 93
BsaJI CCNNGG 3 cut(s) 337, 534, 694
BsaWI WCCGGW 2 cut(s) 83, 491
Bsc4I CCNNNNNNNGG 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 465
Bse3DI GCAATG 1 cut(s) 574
BseAI TCCGGA 1 cut(s) 491
BseBI CCWGG 2 cut(s) 218, 581
BseDI CCNNGG 3 cut(s) 337, 534, 694
BseLI CCNNNNNNNGG 1 cut(s) 122
BseMI GCAATG 1 cut(s) 574
BseNI ACTGG 1 cut(s) 465
BseXI GCAGC 3 cut(s) 237, 385, 416
BsgI GTGCAG 1 cut(s) 621
Bsh1236I CGCG 1 cut(s) 250
BshFI GGCC 2 cut(s) 336, 620
BshNI GGYRCC 1 cut(s) 299
BsiSI CCGG 2 cut(s) 84, 492
BslFI GGGAC 1 cut(s) 616
BslI CCNNNNNNNGG 1 cut(s) 122
BsmAI GTCTC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 616
BsmI GAATGC 1 cut(s) 608
BsnI GGCC 2 cut(s) 336, 620
Bso31I GGTCTC 1 cut(s) 93
Bsp13I TCCGGA 1 cut(s) 491
Bsp1407I TGTACA 1 cut(s) 234
Bsp143I GATC 1 cut(s) 661
BspACI CCGC 1 cut(s) 223
BspANI GGCC 2 cut(s) 336, 620
BspEI TCCGGA 1 cut(s) 491
BspFNI CGCG 1 cut(s) 250
BspHI TCATGA 1 cut(s) 612
BspLI GGNNCC 1 cut(s) 301
BspOI GCTAGC 1 cut(s) 429
BspPI GGATC 1 cut(s) 656
BspT107I GGYRCC 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 93
BsrDI GCAATG 1 cut(s) 574
BsrGI TGTACA 1 cut(s) 234
BsrI ACTGG 1 cut(s) 465
BssECI CCNNGG 3 cut(s) 337, 534, 694
BssMI GATC 1 cut(s) 661
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 3 cut(s) 337, 534, 694
Bst2UI CCWGG 2 cut(s) 218, 581
Bst4CI ACNGT 3 cut(s) 55, 130, 178
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 1 cut(s) 300
BstAPI GCANNNNNTGC 1 cut(s) 517
BstAUI TGTACA 1 cut(s) 234
BstC8I GCNNGC 4 cut(s) 286, 387, 391, 427
BstDEI CTNAG 1 cut(s) 416
BstFNI CGCG 1 cut(s) 250
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 2 cut(s) 252, 302
BstKTI GATC 1 cut(s) 664
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 1 cut(s) 661
BstMWI GCNNNNNNNGC 2 cut(s) 382, 517
BstNI CCWGG 2 cut(s) 218, 581
BstNSI RCATGY 2 cut(s) 142, 266
BstSCI CCNGG 2 cut(s) 216, 579
BstUI CGCG 1 cut(s) 250
BstV1I GCAGC 3 cut(s) 237, 385, 416
BstV2I GAAGAC 1 cut(s) 124
BsuRI GGCC 2 cut(s) 336, 620
BtsIMutI CAGTG 2 cut(s) 126, 638
Cac8I GCNNGC 4 cut(s) 286, 387, 391, 427
CciI TCATGA 1 cut(s) 612
CfoI GCGC 2 cut(s) 252, 302
Cfr13I GGNCC 1 cut(s) 220
CseI GACGC 1 cut(s) 148
Csp6I GTAC 2 cut(s) 235, 531
CviAII CATG 3 cut(s) 139, 263, 613
CviJI RGCY 8 cut(s) 208, 242, 288, 336, 385, 393, 429, 620
CviKI_1 RGCY 8 cut(s) 208, 242, 288, 336, 385, 393, 429, 620
CviQI GTAC 2 cut(s) 235, 531
DdeI CTNAG 1 cut(s) 416
DinI GGCGCC 1 cut(s) 301
DpnI GATC 1 cut(s) 663
DpnII GATC 1 cut(s) 661
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
Eco130I CCWWGG 3 cut(s) 337, 534, 694
Eco147I AGGCCT 1 cut(s) 620
Eco31I GGTCTC 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 220
EcoRII CCWGG 2 cut(s) 216, 579
EcoT14I CCWWGG 3 cut(s) 337, 534, 694
EgeI GGCGCC 1 cut(s) 301
EheI GGCGCC 1 cut(s) 301
ErhI CCWWGG 3 cut(s) 337, 534, 694
FaeI CATG 3 cut(s) 142, 266, 616
FaqI GGGAC 1 cut(s) 616
FatI CATG 3 cut(s) 138, 262, 612
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 3 cut(s) 226, 374, 430
Fsp4HI GCNGC 3 cut(s) 226, 374, 430
FspBI CTAG 2 cut(s) 212, 426
GlaI GCGC 2 cut(s) 251, 301
GluI GCNGC 3 cut(s) 226, 374, 430
GsuI CTGGAG 1 cut(s) 602
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 2 cut(s) 336, 620
HapII CCGG 2 cut(s) 84, 492
HgaI GACGC 1 cut(s) 148
HhaI GCGC 2 cut(s) 252, 302
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 3 cut(s) 142, 266, 616
Hin6I GCGC 2 cut(s) 250, 300
HinP1I GCGC 2 cut(s) 250, 300
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 3 cut(s) 539, 629, 684
HpaII CCGG 2 cut(s) 84, 492
HphI GGTGA 2 cut(s) 16, 73
Hpy166II GTNNAC 3 cut(s) 163, 235, 237
Hpy188I TCNGA 3 cut(s) 98, 270, 325
Hpy188III TCNNGA 3 cut(s) 492, 613, 670
Hpy8I GTNNAC 3 cut(s) 163, 235, 237
HpyAV CCTTC 3 cut(s) 313, 593, 610
HpyCH4III ACNGT 3 cut(s) 55, 130, 178
HpyCH4V TGCA 4 cut(s) 511, 574, 608, 638
HpyF10VI GCNNNNNNNGC 2 cut(s) 382, 517
HpyF3I CTNAG 1 cut(s) 416
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 3 cut(s) 142, 266, 616
HspAI GCGC 2 cut(s) 250, 300
KasI GGCGCC 1 cut(s) 299
Kpn2I TCCGGA 1 cut(s) 491
Kzo9I GATC 1 cut(s) 661
Lsp1109I GCAGC 3 cut(s) 237, 385, 416
LweI GCATC 1 cut(s) 412
MaeI CTAG 2 cut(s) 212, 426
MalI GATC 1 cut(s) 663
MboI GATC 1 cut(s) 661
MboII GAAGA 6 cut(s) 13, 37, 70, 124, 456, 679
MlsI TGGCCA 1 cut(s) 336
MluCI AATT 2 cut(s) 106, 480
MluNI TGGCCA 1 cut(s) 336
Mly113I GGCGCC 1 cut(s) 300
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 5 cut(s) 37, 123, 372, 488, 577
Mox20I TGGCCA 1 cut(s) 336
MroI TCCGGA 1 cut(s) 491
MscI TGGCCA 1 cut(s) 336
MslI CAYNNNNRTG 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 336
MspI CCGG 2 cut(s) 84, 492
MspR9I CCNGG 2 cut(s) 218, 581
Mva1269I GAATGC 1 cut(s) 608
MvaI CCWGG 2 cut(s) 218, 581
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 2 cut(s) 382, 517
NarI GGCGCC 1 cut(s) 300
NdeII GATC 1 cut(s) 661
NheI GCTAGC 1 cut(s) 425
NlaIII CATG 3 cut(s) 142, 266, 616
NlaIV GGNNCC 1 cut(s) 301
NspI RCATGY 2 cut(s) 142, 266
PagI TCATGA 1 cut(s) 612
PceI AGGCCT 1 cut(s) 620
PciI ACATGT 1 cut(s) 138
PctI GAATGC 1 cut(s) 608
PfeI GAWTC 3 cut(s) 539, 629, 684
PfoI TCCNGGA 1 cut(s) 216
PkrI GCNGC 3 cut(s) 227, 375, 431
PluTI GGCGCC 1 cut(s) 303
PscI ACATGT 1 cut(s) 138
Psp6I CCWGG 2 cut(s) 216, 579
PspGI CCWGG 2 cut(s) 216, 579
PspN4I GGNNCC 1 cut(s) 301
PspPI GGNCC 1 cut(s) 220
RsaI GTAC 2 cut(s) 236, 532
RsaNI GTAC 2 cut(s) 235, 531
RseI CAYNNNNRTG 1 cut(s) 35
SatI GCNGC 3 cut(s) 226, 374, 430
Sau3AI GATC 1 cut(s) 661
Sau96I GGNCC 1 cut(s) 220
ScrFI CCNGG 2 cut(s) 218, 581
SetI ASST 9 cut(s) 29, 210, 290, 387, 395, 431, 536, 647, 696
SfaNI GCATC 1 cut(s) 412
SfoI GGCGCC 1 cut(s) 301
SinI GGWCC 1 cut(s) 220
SmiMI CAYNNNNRTG 1 cut(s) 35
Sse9I AATT 2 cut(s) 106, 480
SseBI AGGCCT 1 cut(s) 620
SsiI CCGC 1 cut(s) 223
SspDI GGCGCC 1 cut(s) 299
SspMI CTAG 2 cut(s) 212, 426
StuI AGGCCT 1 cut(s) 620
StyD4I CCNGG 2 cut(s) 216, 579
StyI CCWWGG 3 cut(s) 337, 534, 694
TaaI ACNGT 3 cut(s) 55, 130, 178
TaqI TCGA 3 cut(s) 293, 396, 485
TasI AATT 2 cut(s) 106, 480
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 3 cut(s) 539, 629, 684
TscAI CASTG 2 cut(s) 133, 645
TseI GCWGC 3 cut(s) 225, 373, 429
TspDTI ATGAA 4 cut(s) 55, 601, 629, 642
TspRI CASTG 2 cut(s) 133, 645
VpaK11BI GGWCC 1 cut(s) 220
XapI RAATTY 1 cut(s) 480
XceI RCATGY 2 cut(s) 142, 266
XmiI GTMKAC 1 cut(s) 162
XspI CTAG 2 cut(s) 212, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.