cytochrome P450
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 732 |
| Acc36I | ACCTGC | 1 cut(s) | 1288 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 947 |
| AccII | CGCG | 1 cut(s) | 1191 |
| AciI | CCGC | 4 cut(s) | 408, 521, 1224, 1372 |
| AclWI | GGATC | 3 cut(s) | 542, 1353, 1366 |
| AcoI | YGGCCR | 2 cut(s) | 454, 1373 |
| AcsI | RAATTY | 5 cut(s) | 304, 412, 594, 747, 806 |
| AcuI | CTGAAG | 3 cut(s) | 747, 1121, 1170 |
| AcyI | GRCGYC | 1 cut(s) | 38 |
| AfaI | GTAC | 1 cut(s) | 105 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 194, 947, 1435 |
| AgsI | TTSAA | 3 cut(s) | 297, 569, 904 |
| AjnI | CCWGG | 2 cut(s) | 658, 1397 |
| Alw26I | GTCTC | 1 cut(s) | 141 |
| AlwI | GGATC | 3 cut(s) | 542, 1353, 1366 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 875, 1294 |
| Ama87I | CYCGRG | 1 cut(s) | 780 |
| AoxI | GGCC | 5 cut(s) | 454, 729, 893, 1228, 1373 |
| ApeKI | GCWGC | 7 cut(s) | 122, 869, 872, 875, 1154, 1268, 1277 |
| ApoI | RAATTY | 5 cut(s) | 304, 412, 594, 747, 806 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1464 |
| AspS9I | GGNCC | 3 cut(s) | 680, 1390, 1507 |
| AsuC2I | CCSGG | 1 cut(s) | 1356 |
| AsuHPI | GGTGA | 3 cut(s) | 247, 1004, 1061 |
| AsuII | TTCGAA | 1 cut(s) | 821 |
| AvaI | CYCGRG | 1 cut(s) | 780 |
| AvaII | GGWCC | 3 cut(s) | 680, 1390, 1507 |
| BaeGI | GKGCMC | 1 cut(s) | 1288 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 87, 120 |
| BalI | TGGCCA | 1 cut(s) | 456 |
| BamHI | GGATCC | 1 cut(s) | 1358 |
| BanII | GRGCYC | 1 cut(s) | 266 |
| BbvI | GCAGC | 7 cut(s) | 109, 859, 862, 881, 1141, 1264, 1280 |
| BccI | CCATC | 7 cut(s) | 68, 353, 380, 528, 634, 1316, 1396 |
| BceAI | ACGGC | 1 cut(s) | 25 |
| BciT130I | CCWGG | 2 cut(s) | 660, 1399 |
| BclI | TGATCA | 1 cut(s) | 58 |
| BcnI | CCSGG | 1 cut(s) | 1356 |
| BcoDI | GTCTC | 1 cut(s) | 141 |
| BfaI | CTAG | 3 cut(s) | 258, 279, 429 |
| BfmI | CTRYAG | 5 cut(s) | 180, 315, 539, 963, 1295 |
| BfuAI | ACCTGC | 1 cut(s) | 1288 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 462 |
| BisI | GCNGC | 8 cut(s) | 123, 870, 873, 876, 1155, 1269, 1278, 1373 |
| BlpI | GCTNAGC | 1 cut(s) | 1203 |
| BlsI | GCNGC | 8 cut(s) | 124, 871, 874, 877, 1156, 1270, 1279, 1374 |
| Bme1390I | CCNGG | 3 cut(s) | 660, 1356, 1399 |
| Bme18I | GGWCC | 3 cut(s) | 680, 1390, 1507 |
| BmeT110I | CYCGRG | 1 cut(s) | 780 |
| BmgT120I | GGNCC | 3 cut(s) | 680, 1390, 1507 |
| BmiI | GGNNCC | 3 cut(s) | 364, 657, 1360 |
| BmrFI | CCNGG | 3 cut(s) | 660, 1356, 1399 |
| BmsI | GCATC | 6 cut(s) | 299, 331, 358, 856, 1333, 1432 |
| BpmI | CTGGAG | 2 cut(s) | 216, 270 |
| Bpu10I | CCTNAGC | 1 cut(s) | 225 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1203 |
| Bpu14I | TTCGAA | 1 cut(s) | 821 |
| BpuEI | CTTGAG | 1 cut(s) | 1434 |
| BpuMI | CCSGG | 1 cut(s) | 1356 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1028 |
| BsaHI | GRCGYC | 1 cut(s) | 38 |
| BsaJI | CCNNGG | 2 cut(s) | 478, 676 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 328, 358 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 194, 947, 1435 |
| Bse118I | RCCGGY | 1 cut(s) | 1234 |
| Bse1I | ACTGG | 2 cut(s) | 732, 1247 |
| Bse3DI | GCAATG | 1 cut(s) | 1362 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1028 |
| BseBI | CCWGG | 2 cut(s) | 660, 1399 |
| BseDI | CCNNGG | 2 cut(s) | 478, 676 |
| BseGI | GGATG | 3 cut(s) | 529, 539, 1407 |
| BseJI | GATNNNNATC | 1 cut(s) | 1028 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 194, 947, 1435 |
| BseMI | GCAATG | 1 cut(s) | 1362 |
| BseMII | CTCAG | 3 cut(s) | 152, 239, 1194 |
| BseNI | ACTGG | 2 cut(s) | 732, 1247 |
| BseSI | GKGCMC | 1 cut(s) | 1288 |
| BseXI | GCAGC | 7 cut(s) | 109, 859, 862, 881, 1141, 1264, 1280 |
| BseYI | CCCAGC | 1 cut(s) | 1287 |
| BsgI | GTGCAG | 1 cut(s) | 1287 |
| Bsh1236I | CGCG | 1 cut(s) | 1191 |
| BshFI | GGCC | 5 cut(s) | 456, 731, 895, 1230, 1375 |
| BsiHKCI | CYCGRG | 1 cut(s) | 780 |
| BsiSI | CCGG | 2 cut(s) | 1235, 1355 |
| BslFI | GGGAC | 1 cut(s) | 996 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 194, 947, 1435 |
| BsmAI | GTCTC | 1 cut(s) | 141 |
| BsmFI | GGGAC | 1 cut(s) | 996 |
| BsnI | GGCC | 5 cut(s) | 456, 731, 895, 1230, 1375 |
| BsoBI | CYCGRG | 1 cut(s) | 780 |
| Bsp119I | TTCGAA | 1 cut(s) | 821 |
| Bsp1286I | GDGCHC | 2 cut(s) | 266, 1288 |
| Bsp143I | GATC | 5 cut(s) | 58, 490, 547, 1029, 1358 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1203 |
| Bsp19I | CCATGG | 1 cut(s) | 676 |
| BspACI | CCGC | 4 cut(s) | 408, 521, 1224, 1372 |
| BspANI | GGCC | 5 cut(s) | 456, 731, 895, 1230, 1375 |
| BspCNI | CTCAG | 3 cut(s) | 151, 238, 1195 |
| BspFNI | CGCG | 1 cut(s) | 1191 |
| BspLI | GGNNCC | 3 cut(s) | 364, 657, 1360 |
| BspMAI | CTGCAG | 2 cut(s) | 319, 1299 |
| BspMI | ACCTGC | 1 cut(s) | 1288 |
| BspPI | GGATC | 3 cut(s) | 542, 1353, 1366 |
| BspQI | GCTCTTC | 1 cut(s) | 1470 |
| BspT104I | TTCGAA | 1 cut(s) | 821 |
| BsrDI | GCAATG | 1 cut(s) | 1362 |
| BsrFI | RCCGGY | 1 cut(s) | 1234 |
| BsrI | ACTGG | 2 cut(s) | 732, 1247 |
| BssAI | RCCGGY | 1 cut(s) | 1234 |
| BssECI | CCNNGG | 2 cut(s) | 478, 676 |
| BssMI | GATC | 5 cut(s) | 58, 490, 547, 1029, 1358 |
| BssNI | GRCGYC | 1 cut(s) | 38 |
| BssT1I | CCWWGG | 2 cut(s) | 478, 676 |
| Bst2UI | CCWGG | 2 cut(s) | 660, 1399 |
| Bst4CI | ACNGT | 1 cut(s) | 508 |
| Bst6I | CTCTTC | 4 cut(s) | 564, 1084, 1310, 1470 |
| BstACI | GRCGYC | 1 cut(s) | 38 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 341, 875 |
| BstBI | TTCGAA | 1 cut(s) | 821 |
| BstC8I | GCNNGC | 2 cut(s) | 1059, 1228 |
| BstDEI | CTNAG | 3 cut(s) | 138, 225, 1203 |
| BstDSI | CCRYGG | 1 cut(s) | 676 |
| BstEII | GGTNACC | 1 cut(s) | 235 |
| BstF5I | GGATG | 3 cut(s) | 529, 539, 1407 |
| BstFNI | CGCG | 1 cut(s) | 1191 |
| BstKTI | GATC | 5 cut(s) | 61, 493, 550, 1032, 1361 |
| BstMAI | GTCTC | 1 cut(s) | 141 |
| BstMBI | GATC | 5 cut(s) | 58, 490, 547, 1029, 1358 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 341, 462, 875, 1175, 1274, 1277, 1283 |
| BstNI | CCWGG | 2 cut(s) | 660, 1399 |
| BstNSI | RCATGY | 3 cut(s) | 224, 1202, 1338 |
| BstPI | GGTNACC | 1 cut(s) | 235 |
| BstSCI | CCNGG | 3 cut(s) | 658, 1354, 1397 |
| BstSFI | CTRYAG | 5 cut(s) | 180, 315, 539, 963, 1295 |
| BstSLI | GKGCMC | 1 cut(s) | 1288 |
| BstUI | CGCG | 1 cut(s) | 1191 |
| BstV1I | GCAGC | 7 cut(s) | 109, 859, 862, 881, 1141, 1264, 1280 |
| BstX2I | RGATCY | 1 cut(s) | 1358 |
| BstYI | RGATCY | 1 cut(s) | 1358 |
| BsuRI | GGCC | 5 cut(s) | 456, 731, 895, 1230, 1375 |
| BtgI | CCRYGG | 1 cut(s) | 676 |
| BtsCI | GGATG | 3 cut(s) | 529, 539, 1407 |
| BtsIMutI | CAGTG | 3 cut(s) | 121, 168, 1146 |
| BveI | ACCTGC | 1 cut(s) | 1288 |
| Cac8I | GCNNGC | 2 cut(s) | 1059, 1228 |
| CaiI | CAGNNNCTG | 2 cut(s) | 875, 1294 |
| Cfr10I | RCCGGY | 1 cut(s) | 1234 |
| Cfr13I | GGNCC | 3 cut(s) | 680, 1390, 1507 |
| CseI | GACGC | 1 cut(s) | 46 |
| Csp6I | GTAC | 1 cut(s) | 104 |
| CviQI | GTAC | 1 cut(s) | 104 |
| DdeI | CTNAG | 3 cut(s) | 138, 225, 1203 |
| DpnI | GATC | 5 cut(s) | 60, 492, 549, 1031, 1360 |
| DpnII | GATC | 5 cut(s) | 58, 490, 547, 1029, 1358 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 732 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 732 |
| EaeI | YGGCCR | 2 cut(s) | 454, 1373 |
| Eam1104I | CTCTTC | 4 cut(s) | 564, 1084, 1310, 1470 |
| EarI | CTCTTC | 4 cut(s) | 564, 1084, 1310, 1470 |
| Eco130I | CCWWGG | 2 cut(s) | 478, 676 |
| Eco24I | GRGCYC | 1 cut(s) | 266 |
| Eco32I | GATATC | 2 cut(s) | 607, 970 |
| Eco47I | GGWCC | 3 cut(s) | 680, 1390, 1507 |
| Eco57I | CTGAAG | 3 cut(s) | 747, 1121, 1170 |
| Eco88I | CYCGRG | 1 cut(s) | 780 |
| Eco91I | GGTNACC | 1 cut(s) | 235 |
| EcoO65I | GGTNACC | 1 cut(s) | 235 |
| EcoRI | GAATTC | 1 cut(s) | 806 |
| EcoRII | CCWGG | 2 cut(s) | 658, 1397 |
| EcoRV | GATATC | 2 cut(s) | 607, 970 |
| EcoT14I | CCWWGG | 2 cut(s) | 478, 676 |
| EcoT22I | ATGCAT | 1 cut(s) | 292 |
| EcoT38I | GRGCYC | 1 cut(s) | 266 |
| ErhI | CCWWGG | 2 cut(s) | 478, 676 |
| FaqI | GGGAC | 1 cut(s) | 996 |
| FauI | CCCGC | 2 cut(s) | 415, 1231 |
| FauNDI | CATATG | 1 cut(s) | 943 |
| FbaI | TGATCA | 1 cut(s) | 58 |
| Fnu4HI | GCNGC | 8 cut(s) | 123, 870, 873, 876, 1155, 1269, 1278, 1373 |
| FokI | GGATG | 3 cut(s) | 536, 546, 1414 |
| FriOI | GRGCYC | 1 cut(s) | 266 |
| Fsp4HI | GCNGC | 8 cut(s) | 123, 870, 873, 876, 1155, 1269, 1278, 1373 |
| FspBI | CTAG | 3 cut(s) | 258, 279, 429 |
| GluI | GCNGC | 8 cut(s) | 123, 870, 873, 876, 1155, 1269, 1278, 1373 |
| GsaI | CCCAGC | 1 cut(s) | 1291 |
| GsuI | CTGGAG | 2 cut(s) | 216, 270 |
| HaeIII | GGCC | 5 cut(s) | 456, 731, 895, 1230, 1375 |
| HapII | CCGG | 2 cut(s) | 1235, 1355 |
| HgaI | GACGC | 1 cut(s) | 46 |
| Hin1I | GRCGYC | 1 cut(s) | 38 |
| HinfI | GANTC | 5 cut(s) | 209, 320, 443, 1024, 1326 |
| HpaII | CCGG | 2 cut(s) | 1235, 1355 |
| HphI | GGTGA | 3 cut(s) | 247, 1004, 1061 |
| Hpy166II | GTNNAC | 2 cut(s) | 622, 1003 |
| Hpy188I | TCNGA | 6 cut(s) | 141, 1029, 1101, 1304, 1435, 1470 |
| Hpy188III | TCNNGA | 5 cut(s) | 195, 227, 249, 782, 1064 |
| Hpy8I | GTNNAC | 2 cut(s) | 622, 1003 |
| Hpy99I | CGWCG | 1 cut(s) | 40 |
| HpyAV | CCTTC | 2 cut(s) | 198, 1440 |
| HpyCH4III | ACNGT | 1 cut(s) | 508 |
| HpyCH4IV | ACGT | 1 cut(s) | 1381 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 341, 462, 875, 1175, 1274, 1277, 1283 |
| HpyF3I | CTNAG | 3 cut(s) | 138, 225, 1203 |
| HpySE526I | ACGT | 1 cut(s) | 1381 |
| Hsp92I | GRCGYC | 1 cut(s) | 38 |
| Ksp22I | TGATCA | 1 cut(s) | 58 |
| Kzo9I | GATC | 5 cut(s) | 58, 490, 547, 1029, 1358 |
| LguI | GCTCTTC | 1 cut(s) | 1470 |
| LmnI | GCTCC | 2 cut(s) | 349, 1225 |
| Lsp1109I | GCAGC | 7 cut(s) | 109, 859, 862, 881, 1141, 1264, 1280 |
| LweI | GCATC | 6 cut(s) | 299, 331, 358, 856, 1333, 1432 |
| MaeI | CTAG | 3 cut(s) | 258, 279, 429 |
| MaeII | ACGT | 1 cut(s) | 1381 |
| MaeIII | GTNAC | 2 cut(s) | 235, 1330 |
| MalI | GATC | 5 cut(s) | 60, 492, 549, 1031, 1360 |
| MboI | GATC | 5 cut(s) | 58, 490, 547, 1029, 1358 |
| MfeI | CAATTG | 1 cut(s) | 957 |
| MflI | RGATCY | 1 cut(s) | 1358 |
| MhlI | GDGCHC | 2 cut(s) | 266, 1288 |
| MlsI | TGGCCA | 1 cut(s) | 456 |
| MluNI | TGGCCA | 1 cut(s) | 456 |
| MlyI | GAGTC | 2 cut(s) | 329, 1320 |
| Mox20I | TGGCCA | 1 cut(s) | 456 |
| Mph1103I | ATGCAT | 1 cut(s) | 292 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1464 |
| MscI | TGGCCA | 1 cut(s) | 456 |
| MseI | TTAA | 3 cut(s) | 65, 1044, 1073 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1367 |
| Msp20I | TGGCCA | 1 cut(s) | 456 |
| MspA1I | CMGCKG | 1 cut(s) | 872 |
| MspI | CCGG | 2 cut(s) | 1235, 1355 |
| MspR9I | CCNGG | 3 cut(s) | 660, 1356, 1399 |
| MunI | CAATTG | 1 cut(s) | 957 |
| MvaI | CCWGG | 2 cut(s) | 660, 1399 |
| MvnI | CGCG | 1 cut(s) | 1191 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 341, 462, 875, 1175, 1274, 1277, 1283 |
| NciI | CCSGG | 1 cut(s) | 1356 |
| NcoI | CCATGG | 1 cut(s) | 676 |
| NdeI | CATATG | 1 cut(s) | 943 |
| NdeII | GATC | 5 cut(s) | 58, 490, 547, 1029, 1358 |
| NlaIV | GGNNCC | 3 cut(s) | 364, 657, 1360 |
| NmeAIII | GCCGAG | 1 cut(s) | 1513 |
| NmuCI | GTSAC | 2 cut(s) | 235, 1330 |
| NsiI | ATGCAT | 1 cut(s) | 292 |
| NspI | RCATGY | 3 cut(s) | 224, 1202, 1338 |
| NspV | TTCGAA | 1 cut(s) | 821 |
| PaeR7I | CTCGAG | 1 cut(s) | 780 |
| PciSI | GCTCTTC | 1 cut(s) | 1470 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1464 |
| PfeI | GAWTC | 3 cut(s) | 209, 443, 1024 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 947 |
| PfoI | TCCNGGA | 1 cut(s) | 1354 |
| PkrI | GCNGC | 8 cut(s) | 124, 871, 874, 877, 1156, 1270, 1279, 1374 |
| PleI | GAGTC | 2 cut(s) | 328, 1320 |
| PpsI | GAGTC | 2 cut(s) | 328, 1320 |
| Psp6I | CCWGG | 2 cut(s) | 658, 1397 |
| PspEI | GGTNACC | 1 cut(s) | 235 |
| PspFI | CCCAGC | 1 cut(s) | 1287 |
| PspGI | CCWGG | 2 cut(s) | 658, 1397 |
| PspN4I | GGNNCC | 3 cut(s) | 364, 657, 1360 |
| PspPI | GGNCC | 3 cut(s) | 680, 1390, 1507 |
| PstI | CTGCAG | 2 cut(s) | 319, 1299 |
| PstNI | CAGNNNCTG | 2 cut(s) | 875, 1294 |
| PsuI | RGATCY | 1 cut(s) | 1358 |
| PvuII | CAGCTG | 1 cut(s) | 872 |
| RsaI | GTAC | 1 cut(s) | 105 |
| RsaNI | GTAC | 1 cut(s) | 104 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1367 |
| SapI | GCTCTTC | 1 cut(s) | 1470 |
| SaqAI | TTAA | 3 cut(s) | 65, 1044, 1073 |
| SatI | GCNGC | 8 cut(s) | 123, 870, 873, 876, 1155, 1269, 1278, 1373 |
| Sau3AI | GATC | 5 cut(s) | 58, 490, 547, 1029, 1358 |
| Sau96I | GGNCC | 3 cut(s) | 680, 1390, 1507 |
| SchI | GAGTC | 2 cut(s) | 329, 1320 |
| ScrFI | CCNGG | 3 cut(s) | 660, 1356, 1399 |
| SduI | GDGCHC | 2 cut(s) | 266, 1288 |
| SfaNI | GCATC | 6 cut(s) | 299, 331, 358, 856, 1333, 1432 |
| SfcI | CTRYAG | 5 cut(s) | 180, 315, 539, 963, 1295 |
| Sfr274I | CTCGAG | 1 cut(s) | 780 |
| SfuI | TTCGAA | 1 cut(s) | 821 |
| SinI | GGWCC | 3 cut(s) | 680, 1390, 1507 |
| SlaI | CTCGAG | 1 cut(s) | 780 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1367 |
| SmlI | CTYRAG | 2 cut(s) | 780, 1449 |
| SmoI | CTYRAG | 2 cut(s) | 780, 1449 |
| SsiI | CCGC | 4 cut(s) | 408, 521, 1224, 1372 |
| SspMI | CTAG | 3 cut(s) | 258, 279, 429 |
| StyD4I | CCNGG | 3 cut(s) | 658, 1354, 1397 |
| StyI | CCWWGG | 2 cut(s) | 478, 676 |
| TaaI | ACNGT | 1 cut(s) | 508 |
| TaiI | ACGT | 1 cut(s) | 1384 |
| TaqI | TCGA | 3 cut(s) | 638, 781, 821 |
| TauI | GCSGC | 1 cut(s) | 1375 |
| TfiI | GAWTC | 3 cut(s) | 209, 443, 1024 |
| Tru1I | TTAA | 3 cut(s) | 65, 1044, 1073 |
| Tru9I | TTAA | 3 cut(s) | 65, 1044, 1073 |
| TscAI | CASTG | 3 cut(s) | 121, 175, 1153 |
| TseFI | GTSAC | 2 cut(s) | 235, 1330 |
| TseI | GCWGC | 7 cut(s) | 122, 869, 872, 875, 1154, 1268, 1277 |
| Tsp45I | GTSAC | 2 cut(s) | 235, 1330 |
| TspDTI | ATGAA | 9 cut(s) | 138, 234, 297, 405, 577, 740, 1128, 1401, 1527 |
| TspGWI | ACGGA | 1 cut(s) | 1024 |
| TspRI | CASTG | 3 cut(s) | 121, 175, 1153 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 947 |
| VpaK11BI | GGWCC | 3 cut(s) | 680, 1390, 1507 |
| XapI | RAATTY | 5 cut(s) | 304, 412, 594, 747, 806 |
| XceI | RCATGY | 3 cut(s) | 224, 1202, 1338 |
| XhoI | CTCGAG | 1 cut(s) | 780 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1464 |
| XspI | CTAG | 3 cut(s) | 258, 279, 429 |
| Zsp2I | ATGCAT | 1 cut(s) | 292 |