MD00G1100700.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000357
Physical Location & Seq
Reverse (-)
21348270 .. 21352252
3983 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1100700.v1.1.491

Sequence Viewer

Length: 666 bp
ATGAAAAATGCAATTATCGAAGGAGGAATTCCATTTGAAATGGCTCATGGCTCGCACGCCTTCAAGTACGTAGGATACGACCAGAGGTTTAATCAAGCTTTCAACATAGGAATGAACAGCATCACCACCATGCTTGTAAAGAAGATTCTTGAGGTTTACATGGGTTTTGAGAACCTTAAGAAAGTTGTGGATGTTGGTGGTGGTCTCGGGGTGGCCATTAGTCTAATCACTACCACATATCCTCACATTAAGGGTATCAATTATGATTTACCTCATGTTGTAAAAGATGCTCCTAAATATCCTGGTGTGGAACATGTAGGAGGAGACATGTTTGCAAGTATTCTCGATGCCGATGTTGTTTTTATGAAGGGCATACTTCACGATTGGTTTGATGAAGACAGCATAAAATTGTTGAAAATTTGTTACAAAGCTCTTCCAGTAGATGGAAAAGTGATCGTTGTGGACGCAGTTCTGCCAAGCATCCCAGATAGTAAAAGTATTACGAAAAGCAACTACCTGCTCGATGTACATATGATGGTCCAAACCCCGGGAGGGAAGCAAAGGAGCGCAGAAGAGTTCATGGACTTGGCAACTGCTGCTGATTTTAGTGCCATCAAATTAGCATGCTTTGCGGCCCATCTTGGTGTCGTTGAGTTCTATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01058 ko01100 ko01110 map00940 map01058 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

222

Amino Acids

24.26

Weight (kDa)

6.04

Isoelectric Point (pI)

28.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 2 - 203 4.6e-56 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000357)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17810 FvH4_7g17811 FvH4_7g17820 FvH4_7g17830
malus_domestica MD00G1100700.v1.1 MD01G1089600.v1.1 MD01G1089800.v1.1 MD01G1090400.v1.1 MD01G1090500.v1.1 MD01G1090800.v1.1 MD04G1141100.v1.1 MD07G1161000.v1.1 MD07G1161100.v1.1 MD10G1030000.v1.1 MD10G1030200.v1.1 MD15G1409200.v1.1 MD15G1409700.v1.1
prunus_persica Prupe.2G199100_v2.0.a1 Prupe.2G199100_v2.0.a1 Prupe.2G199300_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199500_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199800_v2.0.a1 Prupe.2G200100_v2.0.a1 Prupe.2G200100_v2.0.a1
pyrus_communis pycom01g11360 pycom01g11370 pycom01g11400 pycom01g11440 pycom01g11480 pycom01g11490 pycom07g15690 pycom08g12100
rosa_chinensis RchiOBHm_Chr1g0317931 RchiOBHm_Chr1g0361211 RchiOBHm_Chr1g0361221 RchiOBHm_Chr1g0361241 RchiOBHm_Chr1g0367691 RchiOBHm_Chr4g0390171 RchiOBHm_Chr4g0396271 RchiOBHm_Chr6g0286421
rosa_laevigata RLG00000002555 RLG00000009555 RLG00000027275 RLG00000027731 RLG00000027732 RLG00000027734 RLG00000027738 RLG00000030575
rosa_multiflora Rmu_co8470451.1_g000001 Rmu_sc0000250.1_g000010 Rmu_sc0010379.1_g000006 Rmu_sc0011132.1_g000005 Rmu_sc0011132.1_g000007 Rmu_sc0038028.1_g000001 Rmu_ssc0000008.1_g000043 Rmu_ssc0000234.1_g000002 Rmu_ssc0000418.1_g000001
rosa_roxburghii Rroxscaffold_2G00116370 Rroxscaffold_4G00289300 Rroxscaffold_4G00295130 Rroxscaffold_4G00295140 Rroxscaffold_4G00295160 Rroxscaffold_4G00295190 Rroxscaffold_4G00295220 Rroxscaffold_4G00330100 Rroxscaffold_5G00341100
rosa_rugosa Rorug01G0287600 Rorug01G0287700 Rorug01G0336200 Rorug01G0336200 Rorug03G0350700
rosa_samantha Rh1AG030300 Rh1AG298500 Rh1AG298600 Rh1AG298700 Rh1AG298900 Rh1BG262400 Rh1BG262500 Rh1BG262800 Rh1BG304800 Rh1CG029000 Rh1CG279800 Rh1CG279900 Rh1CG280000 Rh1CG280100 Rh1CG280200 Rh1CG320500 Rh1DG041200 Rh1DG292700 Rh1DG292800 Rh1DG337100 Rh3DG107500 Rh4AG128100 Rh4BG024900 Rh4CG072700 Rh4DG063000 Rh6CG295900 Rh6DG287700
rosa_wichuraiana Rw1G002290 Rw1G026410 Rw1G026420 Rw1G026440 Rw4G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 525
AccB7I CCANNNNNTGG 1 cut(s) 443
AciI CCGC 1 cut(s) 632
AcoI YGGCCR 1 cut(s) 213
AcsI RAATTY 2 cut(s) 27, 417
AfaI GTAC 2 cut(s) 68, 528
AfiI CCNNNNNNNGG 3 cut(s) 443, 547, 552
AflII CTTAAG 1 cut(s) 176
AflIII ACRYGT 2 cut(s) 313, 327
AgsI TTSAA 4 cut(s) 38, 64, 103, 415
AjnI CCWGG 1 cut(s) 301
AluBI AGCT 2 cut(s) 98, 431
AluI AGCT 2 cut(s) 98, 431
Alw26I GTCTC 2 cut(s) 209, 318
Ama87I CYCGRG 2 cut(s) 206, 547
AoxI GGCC 2 cut(s) 213, 633
ApeKI GCWGC 1 cut(s) 596
ApoI RAATTY 2 cut(s) 27, 417
AspLEI GCGC 1 cut(s) 569
AspS9I GGNCC 2 cut(s) 538, 634
AsuC2I CCSGG 2 cut(s) 548, 549
AsuHPI GGTGA 1 cut(s) 115
AvaI CYCGRG 2 cut(s) 206, 547
AvaII GGWCC 1 cut(s) 538
BalI TGGCCA 1 cut(s) 215
BbsI GAAGAC 1 cut(s) 402
BbvI GCAGC 1 cut(s) 583
BccI CCATC 4 cut(s) 437, 529, 620, 645
BciT130I CCWGG 1 cut(s) 303
BciVI GTATCC 1 cut(s) 68
BcnI CCSGG 2 cut(s) 548, 549
BcoDI GTCTC 2 cut(s) 209, 318
BfrI CTTAAG 1 cut(s) 176
BfuAI ACCTGC 1 cut(s) 525
BfuI GTATCC 1 cut(s) 68
BisI GCNGC 2 cut(s) 597, 633
BlsI GCNGC 2 cut(s) 598, 634
Bme1390I CCNGG 3 cut(s) 303, 548, 549
Bme18I GGWCC 1 cut(s) 538
BmeT110I CYCGRG 2 cut(s) 206, 547
BmgT120I GGNCC 2 cut(s) 538, 634
BmrFI CCNGG 3 cut(s) 303, 548, 549
BmsI GCATC 4 cut(s) 129, 277, 337, 489
BpiI GAAGAC 1 cut(s) 402
BpuEI CTTGAG 1 cut(s) 170
BpuMI CCSGG 2 cut(s) 548, 549
BsaAI YACGTR 1 cut(s) 70
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 2 cut(s) 546, 547
Bsc4I CCNNNNNNNGG 3 cut(s) 443, 547, 552
Bse1I ACTGG 1 cut(s) 437
BseBI CCWGG 1 cut(s) 303
BseDI CCNNGG 2 cut(s) 546, 547
BseGI GGATG 2 cut(s) 196, 480
BseLI CCNNNNNNNGG 3 cut(s) 443, 547, 552
BseNI ACTGG 1 cut(s) 437
BseRI GAGGAG 1 cut(s) 336
BseXI GCAGC 1 cut(s) 583
BshFI GGCC 2 cut(s) 215, 635
BsiHKCI CYCGRG 2 cut(s) 206, 547
BsiSI CCGG 1 cut(s) 548
BslI CCNNNNNNNGG 3 cut(s) 443, 547, 552
BsmAI GTCTC 2 cut(s) 209, 318
BsnI GGCC 2 cut(s) 215, 635
Bso31I GGTCTC 1 cut(s) 209
BsoBI CYCGRG 2 cut(s) 206, 547
Bsp1407I TGTACA 1 cut(s) 526
Bsp143I GATC 1 cut(s) 453
BspACI CCGC 1 cut(s) 632
BspANI GGCC 2 cut(s) 215, 635
BspMI ACCTGC 1 cut(s) 525
BspQI GCTCTTC 1 cut(s) 438
BspTI CTTAAG 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 209
BsrGI TGTACA 1 cut(s) 526
BsrI ACTGG 1 cut(s) 437
BssECI CCNNGG 2 cut(s) 546, 547
BssMI GATC 1 cut(s) 453
Bst2UI CCWGG 1 cut(s) 303
Bst6I CTCTTC 2 cut(s) 438, 567
BstAFI CTTAAG 1 cut(s) 176
BstAPI GCANNNNNTGC 2 cut(s) 596, 629
BstAUI TGTACA 1 cut(s) 526
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 3 cut(s) 53, 57, 625
BstF5I GGATG 2 cut(s) 196, 480
BstHHI GCGC 1 cut(s) 569
BstKTI GATC 1 cut(s) 456
BstMAI GTCTC 2 cut(s) 209, 318
BstMBI GATC 1 cut(s) 453
BstMWI GCNNNNNNNGC 2 cut(s) 596, 629
BstNI CCWGG 1 cut(s) 303
BstNSI RCATGY 3 cut(s) 317, 331, 627
BstSCI CCNGG 3 cut(s) 301, 546, 547
BstSNI TACGTA 1 cut(s) 70
BstV1I GCAGC 1 cut(s) 583
BstV2I GAAGAC 1 cut(s) 402
BsuI GTATCC 1 cut(s) 68
BsuRI GGCC 2 cut(s) 215, 635
BtsCI GGATG 2 cut(s) 196, 480
BveI ACCTGC 1 cut(s) 525
Cac8I GCNNGC 3 cut(s) 53, 57, 625
CfoI GCGC 1 cut(s) 569
Cfr13I GGNCC 2 cut(s) 538, 634
Cfr9I CCCGGG 1 cut(s) 547
CseI GACGC 1 cut(s) 473
Csp6I GTAC 2 cut(s) 67, 527
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
CviAII CATG 8 cut(s) 47, 130, 160, 275, 314, 328, 580, 624
CviJI RGCY 6 cut(s) 44, 51, 98, 215, 431, 635
CviKI_1 RGCY 6 cut(s) 44, 51, 98, 215, 431, 635
CviQI GTAC 2 cut(s) 67, 527
DpnI GATC 1 cut(s) 455
DpnII GATC 1 cut(s) 453
EaeI YGGCCR 1 cut(s) 213
Eam1104I CTCTTC 2 cut(s) 438, 567
EarI CTCTTC 2 cut(s) 438, 567
Eco105I TACGTA 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 538
Eco88I CYCGRG 2 cut(s) 206, 547
EcoRI GAATTC 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 301
FaeI CATG 8 cut(s) 50, 133, 163, 278, 317, 331, 583, 627
FatI CATG 8 cut(s) 46, 129, 159, 274, 313, 327, 579, 623
FauNDI CATATG 1 cut(s) 531
Fnu4HI GCNGC 2 cut(s) 597, 633
FokI GGATG 2 cut(s) 203, 467
Fsp4HI GCNGC 2 cut(s) 597, 633
GlaI GCGC 1 cut(s) 568
GluI GCNGC 2 cut(s) 597, 633
HaeIII GGCC 2 cut(s) 215, 635
HapII CCGG 1 cut(s) 548
HgaI GACGC 1 cut(s) 473
HhaI GCGC 1 cut(s) 569
Hin1II CATG 8 cut(s) 50, 133, 163, 278, 317, 331, 583, 627
Hin6I GCGC 1 cut(s) 567
HinP1I GCGC 1 cut(s) 567
HindIII AAGCTT 1 cut(s) 96
HinfI GANTC 1 cut(s) 145
HpaII CCGG 1 cut(s) 548
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 2 cut(s) 157, 463
Hpy188III TCNNGA 3 cut(s) 149, 344, 380
Hpy8I GTNNAC 2 cut(s) 157, 463
HpyAV CCTTC 3 cut(s) 14, 70, 361
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 2 cut(s) 11, 335
HpyF10VI GCNNNNNNNGC 2 cut(s) 596, 629
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 8 cut(s) 50, 133, 163, 278, 317, 331, 583, 627
HspAI GCGC 1 cut(s) 567
Kzo9I GATC 1 cut(s) 453
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 2 cut(s) 295, 564
LpnPI CCDG 7 cut(s) 95, 288, 315, 450, 498, 530, 561
Lsp1109I GCAGC 1 cut(s) 583
LweI GCATC 4 cut(s) 129, 277, 337, 489
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 1 cut(s) 422
MalI GATC 1 cut(s) 455
MboI GATC 1 cut(s) 453
MboII GAAGA 4 cut(s) 154, 407, 425, 584
MlsI TGGCCA 1 cut(s) 215
MluCI AATT 6 cut(s) 12, 27, 259, 407, 417, 617
MluNI TGGCCA 1 cut(s) 215
MnlI CCTC 7 cut(s) 17, 78, 145, 252, 282, 314, 545
Mox20I TGGCCA 1 cut(s) 215
MscI TGGCCA 1 cut(s) 215
MseI TTAA 3 cut(s) 90, 177, 249
MslI CAYNNNNRTG 3 cut(s) 110, 128, 642
Msp20I TGGCCA 1 cut(s) 215
MspCI CTTAAG 1 cut(s) 176
MspI CCGG 1 cut(s) 548
MspR9I CCNGG 3 cut(s) 303, 548, 549
MvaI CCWGG 1 cut(s) 303
MwoI GCNNNNNNNGC 2 cut(s) 596, 629
NciI CCSGG 2 cut(s) 548, 549
NdeI CATATG 1 cut(s) 531
NdeII GATC 1 cut(s) 453
NlaIII CATG 8 cut(s) 50, 133, 163, 278, 317, 331, 583, 627
NspI RCATGY 3 cut(s) 317, 331, 627
PaeI GCATGC 1 cut(s) 627
PciI ACATGT 2 cut(s) 313, 327
PciSI GCTCTTC 1 cut(s) 438
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PfeI GAWTC 1 cut(s) 145
PflMI CCANNNNNTGG 1 cut(s) 443
PkrI GCNGC 2 cut(s) 598, 634
Ppu21I YACGTR 1 cut(s) 70
PscI ACATGT 2 cut(s) 313, 327
Psp6I CCWGG 1 cut(s) 301
PspGI CCWGG 1 cut(s) 301
PspPI GGNCC 2 cut(s) 538, 634
RsaI GTAC 2 cut(s) 68, 528
RsaNI GTAC 2 cut(s) 67, 527
RseI CAYNNNNRTG 3 cut(s) 110, 128, 642
SapI GCTCTTC 1 cut(s) 438
SaqAI TTAA 3 cut(s) 90, 177, 249
SatI GCNGC 2 cut(s) 597, 633
Sau3AI GATC 1 cut(s) 453
Sau96I GGNCC 2 cut(s) 538, 634
ScrFI CCNGG 3 cut(s) 303, 548, 549
SetI ASST 8 cut(s) 72, 89, 100, 156, 177, 274, 433, 519
SfaNI GCATC 4 cut(s) 129, 277, 337, 489
SinI GGWCC 1 cut(s) 538
SmaI CCCGGG 1 cut(s) 549
SmiMI CAYNNNNRTG 3 cut(s) 110, 128, 642
SmlI CTYRAG 2 cut(s) 149, 176
SmoI CTYRAG 2 cut(s) 149, 176
SnaBI TACGTA 1 cut(s) 70
SphI GCATGC 1 cut(s) 627
Sse9I AATT 6 cut(s) 12, 27, 259, 407, 417, 617
SsiI CCGC 1 cut(s) 632
StyD4I CCNGG 3 cut(s) 301, 546, 547
TaiI ACGT 1 cut(s) 72
TaqI TCGA 3 cut(s) 18, 345, 522
TasI AATT 6 cut(s) 12, 27, 259, 407, 417, 617
TatI WGTACW 1 cut(s) 526
TauI GCSGC 1 cut(s) 635
TfiI GAWTC 1 cut(s) 145
Tru1I TTAA 3 cut(s) 90, 177, 249
Tru9I TTAA 3 cut(s) 90, 177, 249
TseI GCWGC 1 cut(s) 596
TspDTI ATGAA 5 cut(s) 17, 128, 380, 408, 568
TspMI CCCGGG 1 cut(s) 547
Van91I CCANNNNNTGG 1 cut(s) 443
Vha464I CTTAAG 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 538
XapI RAATTY 2 cut(s) 27, 417
XceI RCATGY 3 cut(s) 317, 331, 627
XmaI CCCGGG 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.