Rroxscaffold_4G00295130
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000357
Physical Location & Seq
Forward (+)
15053567 .. 15055025
1459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00295130.1

Sequence Viewer

Length: 975 bp
ATGTCCATGCAATCAGCCATTGAGCTCGGACTTTTTGATATCATAGCCAGAGCAGGTTCAGGTGCGGGGCTCTCTGCACCCCAGATTGCTGCCGAGATTGGCACCCAGAATCCTGAGGCAGCCTTTATGCTGGATCGAATCCTTAGGCTCCTCGCCACTCACTCTGTACTTGGTTGCTCTCTGGTTGATGGCCAAAGGCCCTACAGGCTCTCCGCGGTGTCCAAGCACTTTGTGACTGAAGATGGCGTTTCGTTGGGCTTCGTCATGGCATTGTTCCAAGACAAGGTCTTCATAAACAGTTGGTCACAACTGAAAGACGCAGTTATTGAAGGTGGAATTCCATTTGAGAGGTTCCATGGCATGCCTACCTTTGAGTATTCAGGTTCGGACCCGAGGTTTAATCAGGTTTTCAACACAGCAATGCTCAACCACACCACTCTTGTTGTGAAGCGGATTCTTGATCTCTACAGGGGTTTTGAGCACCTTGGGAAGCTTGTTGATGTTGGTGGTGGTTTGGGAGTAGCTCTCAGTCTAATTACTTCCAGATACCCTCATACTAAGGGAATTAATTACGATCTGCCGCATGTTATAAAATATGCTCACCCTTCTCCTGGTGTCGAACATGTTGGAGGAGACATGTTTTCCCATATTCCAAGTGGAGATGCCATTTTCTTAAAGGGCATACTTCACGATTGGACGGATGAGGACTGCATAAAGTTGTTGAAGAATTGTTACAGTGCTACTCCAGGCGATGGAAAAGTAATCGTTTTGGAGGCAGTTCTTCCAGTTTTGCCGGAAACAAGCACTACTGAGGAGATCACCACCCAACTGGATGTGCTTATGATGACTCAATGCCCAGGAGGGAAGGAGAGGACCCAACAAGAATTCATGGACTTGGCAACTGGTGCTGGATTCAGTGGCATCAAATATGAATGTTTCGTCGCTAATATATGGGTCATGGAGTTTATTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01058 ko01100 ko01110 map00940 map01058 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

324

Amino Acids

35.43

Weight (kDa)

5.43

Isoelectric Point (pI)

29.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 1 - 79 7.9e-16 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 101 - 306 1.7e-64 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000357)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17810 FvH4_7g17811 FvH4_7g17820 FvH4_7g17830
malus_domestica MD00G1100700.v1.1 MD01G1089600.v1.1 MD01G1089800.v1.1 MD01G1090400.v1.1 MD01G1090500.v1.1 MD01G1090800.v1.1 MD04G1141100.v1.1 MD07G1161000.v1.1 MD07G1161100.v1.1 MD10G1030000.v1.1 MD10G1030200.v1.1 MD15G1409200.v1.1 MD15G1409700.v1.1
prunus_persica Prupe.2G199100_v2.0.a1 Prupe.2G199100_v2.0.a1 Prupe.2G199300_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199500_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199800_v2.0.a1 Prupe.2G200100_v2.0.a1 Prupe.2G200100_v2.0.a1
pyrus_communis pycom01g11360 pycom01g11370 pycom01g11400 pycom01g11440 pycom01g11480 pycom01g11490 pycom07g15690 pycom08g12100
rosa_chinensis RchiOBHm_Chr1g0317931 RchiOBHm_Chr1g0361211 RchiOBHm_Chr1g0361221 RchiOBHm_Chr1g0361241 RchiOBHm_Chr1g0367691 RchiOBHm_Chr4g0390171 RchiOBHm_Chr4g0396271 RchiOBHm_Chr6g0286421
rosa_laevigata RLG00000002555 RLG00000009555 RLG00000027275 RLG00000027731 RLG00000027732 RLG00000027734 RLG00000027738 RLG00000030575
rosa_multiflora Rmu_co8470451.1_g000001 Rmu_sc0000250.1_g000010 Rmu_sc0010379.1_g000006 Rmu_sc0011132.1_g000005 Rmu_sc0011132.1_g000007 Rmu_sc0038028.1_g000001 Rmu_ssc0000008.1_g000043 Rmu_ssc0000234.1_g000002 Rmu_ssc0000418.1_g000001
rosa_roxburghii Rroxscaffold_2G00116370 Rroxscaffold_4G00289300 Rroxscaffold_4G00295130 Rroxscaffold_4G00295140 Rroxscaffold_4G00295160 Rroxscaffold_4G00295190 Rroxscaffold_4G00295220 Rroxscaffold_4G00330100 Rroxscaffold_5G00341100
rosa_rugosa Rorug01G0287600 Rorug01G0287700 Rorug01G0336200 Rorug01G0336200 Rorug03G0350700
rosa_samantha Rh1AG030300 Rh1AG298500 Rh1AG298600 Rh1AG298700 Rh1AG298900 Rh1BG262400 Rh1BG262500 Rh1BG262800 Rh1BG304800 Rh1CG029000 Rh1CG279800 Rh1CG279900 Rh1CG280000 Rh1CG280100 Rh1CG280200 Rh1CG320500 Rh1DG041200 Rh1DG292700 Rh1DG292800 Rh1DG337100 Rh3DG107500 Rh4AG128100 Rh4BG024900 Rh4CG072700 Rh4DG063000 Rh6CG295900 Rh6DG287700
rosa_wichuraiana Rw1G002290 Rw1G026410 Rw1G026420 Rw1G026440 Rw4G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 590
Acc36I ACCTGC 1 cut(s) 44
AccB1I GGYRCC 1 cut(s) 101
AccB7I CCANNNNNTGG 1 cut(s) 752
AccII CGCG 1 cut(s) 215
AciI CCGC 5 cut(s) 65, 213, 215, 451, 581
AclWI GGATC 1 cut(s) 141
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 2 cut(s) 336, 884
AcuI CTGAAG 1 cut(s) 258
AdeI CACNNNGTG 1 cut(s) 232
AfaI GTAC 1 cut(s) 168
AfiI CCNNNNNNNGG 3 cut(s) 283, 611, 752
AflIII ACRYGT 2 cut(s) 622, 636
AgsI TTSAA 3 cut(s) 329, 412, 724
AjnI CCWGG 3 cut(s) 610, 745, 856
AluBI AGCT 3 cut(s) 25, 493, 524
AluI AGCT 3 cut(s) 25, 493, 524
Alw21I GWGCWC 2 cut(s) 27, 483
Alw26I GTCTC 1 cut(s) 627
AlwI GGATC 1 cut(s) 141
Ama87I CYCGRG 1 cut(s) 391
AoxI GGCC 2 cut(s) 190, 197
ApeKI GCWGC 2 cut(s) 89, 119
ApoI RAATTY 2 cut(s) 336, 884
AseI ATTAAT 1 cut(s) 567
AspS9I GGNCC 3 cut(s) 198, 388, 873
AsuHPI GGTGA 2 cut(s) 593, 811
AvaI CYCGRG 1 cut(s) 391
AvaII GGWCC 2 cut(s) 388, 873
AxyI CCTNAGG 2 cut(s) 114, 143
BalI TGGCCA 1 cut(s) 192
BanI GGYRCC 1 cut(s) 101
BanII GRGCYC 2 cut(s) 27, 72
BarI GAAGNNNNNNTAC 2 cut(s) 716, 748
BbsI GAAGAC 1 cut(s) 280
Bbv12I GWGCWC 2 cut(s) 27, 483
BbvI GCAGC 2 cut(s) 76, 131
BccI CCATC 3 cut(s) 182, 236, 746
BciT130I CCWGG 3 cut(s) 612, 747, 858
BcoDI GTCTC 1 cut(s) 627
BfmI CTRYAG 2 cut(s) 202, 466
BfuAI ACCTGC 1 cut(s) 44
BglI GCCNNNNNGGC 1 cut(s) 205
BisI GCNGC 3 cut(s) 90, 120, 581
BlsI GCNGC 3 cut(s) 91, 121, 582
Bme1390I CCNGG 3 cut(s) 612, 747, 858
Bme18I GGWCC 2 cut(s) 388, 873
BmeT110I CYCGRG 1 cut(s) 391
BmgT120I GGNCC 3 cut(s) 198, 388, 873
BmiI GGNNCC 5 cut(s) 103, 149, 353, 390, 875
BmrFI CCNGG 3 cut(s) 612, 747, 858
BmsI GCATC 2 cut(s) 652, 930
BpiI GAAGAC 1 cut(s) 280
BplI GAGNNNNNCTC 2 cut(s) 510, 542
BpmI CTGGAG 1 cut(s) 729
BsaJI CCNNGG 5 cut(s) 213, 355, 392, 484, 856
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 611, 752
Bse1I ACTGG 3 cut(s) 785, 834, 907
Bse21I CCTNAGG 2 cut(s) 114, 143
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 3 cut(s) 612, 747, 858
BseDI CCNNGG 5 cut(s) 213, 355, 392, 484, 856
BseGI GGATG 2 cut(s) 706, 838
BseLI CCNNNNNNNGG 3 cut(s) 283, 611, 752
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 3 cut(s) 105, 541, 801
BseNI ACTGG 3 cut(s) 785, 834, 907
BseRI GAGGAG 3 cut(s) 140, 645, 827
BseXI GCAGC 2 cut(s) 76, 131
BsgI GTGCAG 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 215
BshFI GGCC 2 cut(s) 192, 199
BshNI GGYRCC 1 cut(s) 101
BsiHKAI GWGCWC 2 cut(s) 27, 483
BsiHKCI CYCGRG 1 cut(s) 391
BsiSI CCGG 1 cut(s) 794
BslI CCNNNNNNNGG 3 cut(s) 283, 611, 752
BsmAI GTCTC 1 cut(s) 627
BsnI GGCC 2 cut(s) 192, 199
BsoBI CYCGRG 1 cut(s) 391
Bsp1286I GDGCHC 3 cut(s) 27, 72, 483
Bsp143I GATC 4 cut(s) 133, 460, 574, 816
Bsp19I CCATGG 1 cut(s) 355
BspACI CCGC 5 cut(s) 65, 213, 215, 451, 581
BspANI GGCC 2 cut(s) 192, 199
BspCNI CTCAG 3 cut(s) 106, 540, 802
BspFNI CGCG 1 cut(s) 215
BspLI GGNNCC 5 cut(s) 103, 149, 353, 390, 875
BspMI ACCTGC 1 cut(s) 44
BspPI GGATC 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 426
BsrI ACTGG 3 cut(s) 785, 834, 907
BssECI CCNNGG 5 cut(s) 213, 355, 392, 484, 856
BssMI GATC 4 cut(s) 133, 460, 574, 816
BssT1I CCWWGG 2 cut(s) 355, 484
Bst2UI CCWGG 3 cut(s) 612, 747, 858
Bst4CI ACNGT 2 cut(s) 299, 737
BstAPI GCANNNNNTGC 1 cut(s) 905
BstC8I GCNNGC 1 cut(s) 362
BstDEI CTNAG 5 cut(s) 114, 143, 527, 558, 810
BstDSI CCRYGG 2 cut(s) 213, 355
BstF5I GGATG 2 cut(s) 706, 838
BstFNI CGCG 1 cut(s) 215
BstKTI GATC 4 cut(s) 136, 463, 577, 819
BstMAI GTCTC 1 cut(s) 627
BstMBI GATC 4 cut(s) 133, 460, 574, 816
BstMWI GCNNNNNNNGC 2 cut(s) 205, 905
BstNI CCWGG 3 cut(s) 612, 747, 858
BstNSI RCATGY 4 cut(s) 364, 587, 626, 640
BstSCI CCNGG 3 cut(s) 610, 745, 856
BstSFI CTRYAG 2 cut(s) 202, 466
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 2 cut(s) 76, 131
BstV2I GAAGAC 1 cut(s) 280
BstXI CCANNNNNNTGG 1 cut(s) 829
Bsu36I CCTNAGG 2 cut(s) 114, 143
BsuRI GGCC 2 cut(s) 192, 199
BtgI CCRYGG 2 cut(s) 213, 355
BtgZI GCGATG 1 cut(s) 765
BtsCI GGATG 2 cut(s) 706, 838
BtsIMutI CAGTG 2 cut(s) 742, 922
BveI ACCTGC 1 cut(s) 44
Cac8I GCNNGC 1 cut(s) 362
Cfr13I GGNCC 3 cut(s) 198, 388, 873
Cfr42I CCGCGG 1 cut(s) 216
CseI GACGC 1 cut(s) 326
Csp6I GTAC 1 cut(s) 167
CspCI CAANNNNNGTGG 2 cut(s) 424, 459
CviAII CATG 9 cut(s) 7, 265, 356, 361, 584, 623, 637, 889, 958
CviQI GTAC 1 cut(s) 167
DdeI CTNAG 5 cut(s) 114, 143, 527, 558, 810
DpnI GATC 4 cut(s) 135, 462, 576, 818
DpnII GATC 4 cut(s) 133, 460, 574, 816
DraIII CACNNNGTG 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 190
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 2 cut(s) 355, 484
Eco24I GRGCYC 2 cut(s) 27, 72
Eco32I GATATC 1 cut(s) 40
Eco47I GGWCC 2 cut(s) 388, 873
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 258
Eco81I CCTNAGG 2 cut(s) 114, 143
Eco88I CYCGRG 1 cut(s) 391
EcoICRI GAGCTC 1 cut(s) 25
EcoO109I RGGNCCY 2 cut(s) 198, 873
EcoRI GAATTC 2 cut(s) 336, 884
EcoRII CCWGG 3 cut(s) 610, 745, 856
EcoRV GATATC 1 cut(s) 40
EcoT14I CCWWGG 2 cut(s) 355, 484
EcoT38I GRGCYC 2 cut(s) 27, 72
ErhI CCWWGG 2 cut(s) 355, 484
FaeI CATG 9 cut(s) 10, 268, 359, 364, 587, 626, 640, 892, 961
FatI CATG 9 cut(s) 6, 264, 355, 360, 583, 622, 636, 888, 957
FauI CCCGC 1 cut(s) 58
Fnu4HI GCNGC 3 cut(s) 90, 120, 581
FokI GGATG 2 cut(s) 713, 845
FriOI GRGCYC 2 cut(s) 27, 72
Fsp4HI GCNGC 3 cut(s) 90, 120, 581
GluI GCNGC 3 cut(s) 90, 120, 581
GsuI CTGGAG 1 cut(s) 729
HaeIII GGCC 2 cut(s) 192, 199
HapII CCGG 1 cut(s) 794
HgaI GACGC 1 cut(s) 326
Hin1II CATG 9 cut(s) 10, 268, 359, 364, 587, 626, 640, 892, 961
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 5 cut(s) 109, 138, 454, 847, 912
HpaII CCGG 1 cut(s) 794
HphI GGTGA 2 cut(s) 593, 811
Hpy188I TCNGA 2 cut(s) 29, 388
Hpy188III TCNNGA 4 cut(s) 113, 458, 543, 689
Hpy99I CGWCG 1 cut(s) 944
HpyAV CCTTC 3 cut(s) 323, 615, 859
HpyCH4III ACNGT 2 cut(s) 299, 737
HpyCH4V TGCA 3 cut(s) 10, 77, 711
HpyF10VI GCNNNNNNNGC 2 cut(s) 205, 905
HpyF3I CTNAG 5 cut(s) 114, 143, 527, 558, 810
Hsp92II CATG 9 cut(s) 10, 268, 359, 364, 587, 626, 640, 892, 961
KspI CCGCGG 1 cut(s) 216
Kzo9I GATC 4 cut(s) 133, 460, 574, 816
LmnI GCTCC 1 cut(s) 153
Lsp1109I GCAGC 2 cut(s) 76, 131
LweI GCATC 2 cut(s) 652, 930
MaeIII GTNAC 3 cut(s) 232, 303, 731
MalI GATC 4 cut(s) 135, 462, 576, 818
MboI GATC 4 cut(s) 133, 460, 574, 816
MboII GAAGA 4 cut(s) 251, 280, 736, 773
MhlI GDGCHC 3 cut(s) 27, 72, 483
MlsI TGGCCA 1 cut(s) 192
MluCI AATT 6 cut(s) 336, 534, 564, 568, 727, 884
MluNI TGGCCA 1 cut(s) 192
MlyI GAGTC 1 cut(s) 841
MmeI TCCRAC 1 cut(s) 607
Mox20I TGGCCA 1 cut(s) 192
MscI TGGCCA 1 cut(s) 192
MseI TTAA 4 cut(s) 399, 567, 674, 969
MslI CAYNNNNRTG 1 cut(s) 419
Msp20I TGGCCA 1 cut(s) 192
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 1 cut(s) 794
MspR9I CCNGG 3 cut(s) 612, 747, 858
MvaI CCWGG 3 cut(s) 612, 747, 858
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 2 cut(s) 205, 905
NcoI CCATGG 1 cut(s) 355
NdeII GATC 4 cut(s) 133, 460, 574, 816
NlaIII CATG 9 cut(s) 10, 268, 359, 364, 587, 626, 640, 892, 961
NlaIV GGNNCC 5 cut(s) 103, 149, 353, 390, 875
NmeAIII GCCGAG 1 cut(s) 118
NmuCI GTSAC 2 cut(s) 232, 303
NspI RCATGY 4 cut(s) 364, 587, 626, 640
PaeI GCATGC 1 cut(s) 364
PciI ACATGT 2 cut(s) 622, 636
PfeI GAWTC 4 cut(s) 109, 138, 454, 912
PflFI GACNNNGTC 1 cut(s) 284
PflMI CCANNNNNTGG 1 cut(s) 752
PkrI GCNGC 3 cut(s) 91, 121, 582
PleI GAGTC 1 cut(s) 841
PpsI GAGTC 1 cut(s) 841
PpuMI RGGWCCY 1 cut(s) 873
PscI ACATGT 2 cut(s) 622, 636
PshBI ATTAAT 1 cut(s) 567
PsiI TTATAA 1 cut(s) 590
Psp124BI GAGCTC 1 cut(s) 27
Psp5II RGGWCCY 1 cut(s) 873
Psp6I CCWGG 3 cut(s) 610, 745, 856
PspGI CCWGG 3 cut(s) 610, 745, 856
PspN4I GGNNCC 5 cut(s) 103, 149, 353, 390, 875
PspPI GGNCC 3 cut(s) 198, 388, 873
PspPPI RGGWCCY 1 cut(s) 873
PsyI GACNNNGTC 1 cut(s) 284
RsaI GTAC 1 cut(s) 168
RsaNI GTAC 1 cut(s) 167
RseI CAYNNNNRTG 1 cut(s) 419
SacI GAGCTC 1 cut(s) 27
SacII CCGCGG 1 cut(s) 216
SaqAI TTAA 4 cut(s) 399, 567, 674, 969
SatI GCNGC 3 cut(s) 90, 120, 581
Sau3AI GATC 4 cut(s) 133, 460, 574, 816
Sau96I GGNCC 3 cut(s) 198, 388, 873
SchI GAGTC 1 cut(s) 841
ScrFI CCNGG 3 cut(s) 612, 747, 858
SduI GDGCHC 3 cut(s) 27, 72, 483
SfaNI GCATC 2 cut(s) 652, 930
SfcI CTRYAG 2 cut(s) 202, 466
Sfr303I CCGCGG 1 cut(s) 216
SgrBI CCGCGG 1 cut(s) 216
SinI GGWCC 2 cut(s) 388, 873
SmiMI CAYNNNNRTG 1 cut(s) 419
SphI GCATGC 1 cut(s) 364
Sse9I AATT 6 cut(s) 336, 534, 564, 568, 727, 884
SsiI CCGC 5 cut(s) 65, 213, 215, 451, 581
SstI GAGCTC 1 cut(s) 27
StyD4I CCNGG 3 cut(s) 610, 745, 856
StyI CCWWGG 2 cut(s) 355, 484
TaaI ACNGT 2 cut(s) 299, 737
TaqI TCGA 2 cut(s) 136, 618
TasI AATT 6 cut(s) 336, 534, 564, 568, 727, 884
TatI WGTACW 1 cut(s) 166
TauI GCSGC 1 cut(s) 583
TfiI GAWTC 4 cut(s) 109, 138, 454, 912
Tru1I TTAA 4 cut(s) 399, 567, 674, 969
Tru9I TTAA 4 cut(s) 399, 567, 674, 969
TscAI CASTG 2 cut(s) 742, 922
TseFI GTSAC 2 cut(s) 232, 303
TseI GCWGC 2 cut(s) 89, 119
Tsp45I GTSAC 2 cut(s) 232, 303
TspDTI ATGAA 3 cut(s) 280, 877, 945
TspGWI ACGGA 1 cut(s) 713
TspRI CASTG 2 cut(s) 742, 922
Tth111I GACNNNGTC 1 cut(s) 284
Van91I CCANNNNNTGG 1 cut(s) 752
VpaK11BI GGWCC 2 cut(s) 388, 873
VspI ATTAAT 1 cut(s) 567
XapI RAATTY 2 cut(s) 336, 884
XceI RCATGY 4 cut(s) 364, 587, 626, 640
XcmI CCANNNNNNNNNTGG 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.