Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 728 |
| AccB1I | GGYRCC | 1 cut(s) | 218 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 890 |
| AccI | GTMKAC | 1 cut(s) | 525 |
| AccIII | TCCGGA | 1 cut(s) | 327 |
| AclWI | GGATC | 1 cut(s) | 978 |
| AcoI | YGGCCR | 2 cut(s) | 270, 354 |
| AcsI | RAATTY | 4 cut(s) | 36, 591, 1022, 1100 |
| AdeI | CACNNNGTG | 1 cut(s) | 367 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 890, 999 |
| AflIII | ACRYGT | 1 cut(s) | 774 |
| AgsI | TTSAA | 8 cut(s) | 416, 467, 550, 565, 758, 782, 814, 862 |
| AjnI | CCWGG | 3 cut(s) | 267, 517, 748 |
| AluBI | AGCT | 7 cut(s) | 75, 94, 142, 187, 263, 438, 631 |
| AluI | AGCT | 7 cut(s) | 75, 94, 142, 187, 263, 438, 631 |
| Alw21I | GWGCWC | 1 cut(s) | 621 |
| Alw26I | GTCTC | 1 cut(s) | 765 |
| AlwI | GGATC | 1 cut(s) | 978 |
| Ama87I | CYCGRG | 1 cut(s) | 994 |
| Aor13HI | TCCGGA | 1 cut(s) | 327 |
| AoxI | GGCC | 5 cut(s) | 25, 270, 354, 440, 1029 |
| ApeKI | GCWGC | 3 cut(s) | 91, 628, 1079 |
| ApoI | RAATTY | 4 cut(s) | 36, 591, 1022, 1100 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 786 |
| AspS9I | GGNCC | 4 cut(s) | 173, 236, 954, 1030 |
| AsuC2I | CCSGG | 2 cut(s) | 995, 996 |
| AvaI | CYCGRG | 1 cut(s) | 994 |
| AvaII | GGWCC | 3 cut(s) | 173, 236, 954 |
| AxyI | CCTNAGG | 1 cut(s) | 530 |
| BalI | TGGCCA | 2 cut(s) | 272, 356 |
| BanI | GGYRCC | 1 cut(s) | 218 |
| BbsI | GAAGAC | 2 cut(s) | 418, 849 |
| Bbv12I | GWGCWC | 1 cut(s) | 621 |
| BbvI | GCAGC | 3 cut(s) | 103, 640, 1066 |
| BccI | CCATC | 3 cut(s) | 374, 884, 997 |
| BciT130I | CCWGG | 3 cut(s) | 269, 519, 750 |
| BciVI | GTATCC | 1 cut(s) | 525 |
| BcnI | CCSGG | 2 cut(s) | 995, 996 |
| BcoDI | GTCTC | 1 cut(s) | 765 |
| BfaI | CTAG | 4 cut(s) | 248, 260, 444, 1034 |
| BfmI | CTRYAG | 1 cut(s) | 89 |
| BfuI | GTATCC | 1 cut(s) | 525 |
| BisI | GCNGC | 3 cut(s) | 92, 629, 1080 |
| BlsI | GCNGC | 3 cut(s) | 93, 630, 1081 |
| Bme1390I | CCNGG | 5 cut(s) | 269, 519, 750, 995, 996 |
| Bme18I | GGWCC | 3 cut(s) | 173, 236, 954 |
| BmeT110I | CYCGRG | 1 cut(s) | 994 |
| BmgT120I | GGNCC | 4 cut(s) | 173, 236, 954, 1030 |
| BmiI | GGNNCC | 5 cut(s) | 18, 174, 220, 238, 1013 |
| BmrFI | CCNGG | 5 cut(s) | 269, 519, 750, 995, 996 |
| BmsI | GCATC | 5 cut(s) | 203, 234, 445, 790, 1068 |
| BpiI | GAAGAC | 2 cut(s) | 418, 849 |
| BpmI | CTGGAG | 1 cut(s) | 32 |
| BpuMI | CCSGG | 2 cut(s) | 995, 996 |
| BsaJI | CCNNGG | 4 cut(s) | 493, 749, 993, 994 |
| BsaWI | WCCGGW | 1 cut(s) | 327 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 890, 999 |
| Bse1I | ACTGG | 2 cut(s) | 972, 1045 |
| Bse21I | CCTNAGG | 1 cut(s) | 530 |
| Bse3DI | GCAATG | 1 cut(s) | 564 |
| BseAI | TCCGGA | 1 cut(s) | 327 |
| BseBI | CCWGG | 3 cut(s) | 269, 519, 750 |
| BseDI | CCNNGG | 4 cut(s) | 493, 749, 993, 994 |
| BseGI | GGATG | 2 cut(s) | 805, 1008 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 890, 999 |
| BseMI | GCAATG | 1 cut(s) | 564 |
| BseMII | CTCAG | 1 cut(s) | 189 |
| BseNI | ACTGG | 2 cut(s) | 972, 1045 |
| BseRI | GAGGAG | 2 cut(s) | 783, 1024 |
| BseXI | GCAGC | 3 cut(s) | 103, 640, 1066 |
| BshFI | GGCC | 5 cut(s) | 27, 272, 356, 442, 1031 |
| BshNI | GGYRCC | 1 cut(s) | 218 |
| BsiHKAI | GWGCWC | 1 cut(s) | 621 |
| BsiHKCI | CYCGRG | 1 cut(s) | 994 |
| BsiSI | CCGG | 2 cut(s) | 328, 995 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 890, 999 |
| BsmAI | GTCTC | 1 cut(s) | 765 |
| BsnI | GGCC | 5 cut(s) | 27, 272, 356, 442, 1031 |
| BsoBI | CYCGRG | 1 cut(s) | 994 |
| Bsp1286I | GDGCHC | 1 cut(s) | 621 |
| Bsp13I | TCCGGA | 1 cut(s) | 327 |
| Bsp143I | GATC | 2 cut(s) | 250, 970 |
| Bsp19I | CCATGG | 1 cut(s) | 493 |
| BspANI | GGCC | 5 cut(s) | 27, 272, 356, 442, 1031 |
| BspCNI | CTCAG | 1 cut(s) | 190 |
| BspEI | TCCGGA | 1 cut(s) | 327 |
| BspHI | TCATGA | 1 cut(s) | 826 |
| BspLI | GGNNCC | 5 cut(s) | 18, 174, 220, 238, 1013 |
| BspMAI | CTGCAG | 1 cut(s) | 93 |
| BspPI | GGATC | 1 cut(s) | 978 |
| BspT107I | GGYRCC | 1 cut(s) | 218 |
| BsrDI | GCAATG | 1 cut(s) | 564 |
| BsrI | ACTGG | 2 cut(s) | 972, 1045 |
| BssECI | CCNNGG | 4 cut(s) | 493, 749, 993, 994 |
| BssMI | GATC | 2 cut(s) | 250, 970 |
| BssT1I | CCWWGG | 1 cut(s) | 493 |
| Bst2UI | CCWGG | 3 cut(s) | 269, 519, 750 |
| Bst4CI | ACNGT | 2 cut(s) | 298, 308 |
| Bst6I | CTCTTC | 2 cut(s) | 63, 581 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1043 |
| BstC8I | GCNNGC | 3 cut(s) | 274, 440, 500 |
| BstDEI | CTNAG | 2 cut(s) | 198, 530 |
| BstDSI | CCRYGG | 1 cut(s) | 493 |
| BstF5I | GGATG | 2 cut(s) | 805, 1008 |
| BstKTI | GATC | 2 cut(s) | 253, 973 |
| BstMAI | GTCTC | 1 cut(s) | 765 |
| BstMBI | GATC | 2 cut(s) | 250, 970 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 269, 625, 1043 |
| BstNI | CCWGG | 3 cut(s) | 269, 519, 750 |
| BstNSI | RCATGY | 3 cut(s) | 502, 737, 778 |
| BstSCI | CCNGG | 5 cut(s) | 267, 517, 748, 993, 994 |
| BstSFI | CTRYAG | 1 cut(s) | 89 |
| BstV1I | GCAGC | 3 cut(s) | 103, 640, 1066 |
| BstV2I | GAAGAC | 2 cut(s) | 418, 849 |
| BstX2I | RGATCY | 1 cut(s) | 970 |
| BstYI | RGATCY | 1 cut(s) | 970 |
| Bsu36I | CCTNAGG | 1 cut(s) | 530 |
| BsuI | GTATCC | 1 cut(s) | 525 |
| BsuRI | GGCC | 5 cut(s) | 27, 272, 356, 442, 1031 |
| BtgI | CCRYGG | 1 cut(s) | 493 |
| BtsCI | GGATG | 2 cut(s) | 805, 1008 |
| BtsI | GCAGTG | 1 cut(s) | 86 |
| BtsIMutI | CAGTG | 1 cut(s) | 86 |
| Cac8I | GCNNGC | 3 cut(s) | 274, 440, 500 |
| CciI | TCATGA | 1 cut(s) | 826 |
| Cfr13I | GGNCC | 4 cut(s) | 173, 236, 954, 1030 |
| Cfr9I | CCCGGG | 1 cut(s) | 994 |
| DdeI | CTNAG | 2 cut(s) | 198, 530 |
| DpnI | GATC | 2 cut(s) | 252, 972 |
| DpnII | GATC | 2 cut(s) | 250, 970 |
| DraIII | CACNNNGTG | 1 cut(s) | 367 |
| EaeI | YGGCCR | 2 cut(s) | 270, 354 |
| Eam1104I | CTCTTC | 2 cut(s) | 63, 581 |
| EarI | CTCTTC | 2 cut(s) | 63, 581 |
| Eco130I | CCWWGG | 1 cut(s) | 493 |
| Eco147I | AGGCCT | 1 cut(s) | 27 |
| Eco47I | GGWCC | 3 cut(s) | 173, 236, 954 |
| Eco81I | CCTNAGG | 1 cut(s) | 530 |
| Eco88I | CYCGRG | 1 cut(s) | 994 |
| EcoRI | GAATTC | 3 cut(s) | 591, 1022, 1100 |
| EcoRII | CCWGG | 3 cut(s) | 267, 517, 748 |
| EcoT14I | CCWWGG | 1 cut(s) | 493 |
| ErhI | CCWWGG | 1 cut(s) | 493 |
| FauNDI | CATATG | 1 cut(s) | 978 |
| FblI | GTMKAC | 1 cut(s) | 525 |
| Fnu4HI | GCNGC | 3 cut(s) | 92, 629, 1080 |
| FokI | GGATG | 2 cut(s) | 812, 1015 |
| Fsp4HI | GCNGC | 3 cut(s) | 92, 629, 1080 |
| FspBI | CTAG | 4 cut(s) | 248, 260, 444, 1034 |
| GluI | GCNGC | 3 cut(s) | 92, 629, 1080 |
| GsuI | CTGGAG | 1 cut(s) | 32 |
| HaeIII | GGCC | 5 cut(s) | 27, 272, 356, 442, 1031 |
| HapII | CCGG | 2 cut(s) | 328, 995 |
| HincII | GTYRAC | 4 cut(s) | 304, 316, 405, 526 |
| HindII | GTYRAC | 4 cut(s) | 304, 316, 405, 526 |
| HinfI | GANTC | 2 cut(s) | 702, 985 |
| HpaI | GTTAAC | 1 cut(s) | 304 |
| HpaII | CCGG | 2 cut(s) | 328, 995 |
| Hpy166II | GTNNAC | 4 cut(s) | 304, 316, 405, 526 |
| Hpy188I | TCNGA | 2 cut(s) | 199, 1064 |
| Hpy188III | TCNNGA | 6 cut(s) | 11, 44, 328, 416, 827, 884 |
| Hpy8I | GTNNAC | 4 cut(s) | 304, 316, 405, 526 |
| HpyAV | CCTTC | 3 cut(s) | 38, 266, 461 |
| HpyCH4III | ACNGT | 2 cut(s) | 298, 308 |
| HpyCH4IV | ACGT | 1 cut(s) | 621 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 269, 625, 1043 |
| HpyF3I | CTNAG | 2 cut(s) | 198, 530 |
| HpySE526I | ACGT | 1 cut(s) | 621 |
| Kpn2I | TCCGGA | 1 cut(s) | 327 |
| KspAI | GTTAAC | 1 cut(s) | 304 |
| Kzo9I | GATC | 2 cut(s) | 250, 970 |
| LmnI | GCTCC | 1 cut(s) | 1011 |
| Lsp1109I | GCAGC | 3 cut(s) | 103, 640, 1066 |
| LweI | GCATC | 5 cut(s) | 203, 234, 445, 790, 1068 |
| MaeI | CTAG | 4 cut(s) | 248, 260, 444, 1034 |
| MaeII | ACGT | 1 cut(s) | 621 |
| MaeIII | GTNAC | 2 cut(s) | 367, 869 |
| MalI | GATC | 2 cut(s) | 252, 972 |
| MboI | GATC | 2 cut(s) | 250, 970 |
| MboII | GAAGA | 9 cut(s) | 77, 80, 96, 389, 418, 587, 598, 854, 1031 |
| MfeI | CAATTG | 1 cut(s) | 135 |
| MflI | RGATCY | 1 cut(s) | 970 |
| MhlI | GDGCHC | 1 cut(s) | 621 |
| MlsI | TGGCCA | 2 cut(s) | 272, 356 |
| MluNI | TGGCCA | 2 cut(s) | 272, 356 |
| MlyI | GAGTC | 1 cut(s) | 979 |
| MmeI | TCCRAC | 1 cut(s) | 745 |
| Mox20I | TGGCCA | 2 cut(s) | 272, 356 |
| MroI | TCCGGA | 1 cut(s) | 327 |
| MroXI | GAANNNNTTC | 1 cut(s) | 786 |
| MscI | TGGCCA | 2 cut(s) | 272, 356 |
| MseI | TTAA | 5 cut(s) | 303, 431, 537, 624, 696 |
| MslI | CAYNNNNRTG | 2 cut(s) | 581, 947 |
| Msp20I | TGGCCA | 2 cut(s) | 272, 356 |
| MspA1I | CMGCKG | 1 cut(s) | 94 |
| MspI | CCGG | 2 cut(s) | 328, 995 |
| MspR9I | CCNGG | 5 cut(s) | 269, 519, 750, 995, 996 |
| MunI | CAATTG | 1 cut(s) | 135 |
| MvaI | CCWGG | 3 cut(s) | 269, 519, 750 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 269, 625, 1043 |
| NciI | CCSGG | 2 cut(s) | 995, 996 |
| NcoI | CCATGG | 1 cut(s) | 493 |
| NdeI | CATATG | 1 cut(s) | 978 |
| NdeII | GATC | 2 cut(s) | 250, 970 |
| NlaIV | GGNNCC | 5 cut(s) | 18, 174, 220, 238, 1013 |
| NmuCI | GTSAC | 1 cut(s) | 367 |
| NspI | RCATGY | 3 cut(s) | 502, 737, 778 |
| PaeI | GCATGC | 1 cut(s) | 502 |
| PagI | TCATGA | 1 cut(s) | 826 |
| PceI | AGGCCT | 1 cut(s) | 27 |
| PciI | ACATGT | 1 cut(s) | 774 |
| PdmI | GAANNNNTTC | 1 cut(s) | 786 |
| PfeI | GAWTC | 1 cut(s) | 702 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 890 |
| PkrI | GCNGC | 3 cut(s) | 93, 630, 1081 |
| PleI | GAGTC | 1 cut(s) | 979 |
| PpsI | GAGTC | 1 cut(s) | 979 |
| PscI | ACATGT | 1 cut(s) | 774 |
| PsiI | TTATAA | 1 cut(s) | 728 |
| Psp6I | CCWGG | 3 cut(s) | 267, 517, 748 |
| PspGI | CCWGG | 3 cut(s) | 267, 517, 748 |
| PspN4I | GGNNCC | 5 cut(s) | 18, 174, 220, 238, 1013 |
| PspPI | GGNCC | 4 cut(s) | 173, 236, 954, 1030 |
| PstI | CTGCAG | 1 cut(s) | 93 |
| PsuI | RGATCY | 1 cut(s) | 970 |
| PvuII | CAGCTG | 1 cut(s) | 94 |
| RseI | CAYNNNNRTG | 2 cut(s) | 581, 947 |
| SalI | GTCGAC | 1 cut(s) | 524 |
| SaqAI | TTAA | 5 cut(s) | 303, 431, 537, 624, 696 |
| SatI | GCNGC | 3 cut(s) | 92, 629, 1080 |
| Sau3AI | GATC | 2 cut(s) | 250, 970 |
| Sau96I | GGNCC | 4 cut(s) | 173, 236, 954, 1030 |
| SchI | GAGTC | 1 cut(s) | 979 |
| ScrFI | CCNGG | 5 cut(s) | 269, 519, 750, 995, 996 |
| SduI | GDGCHC | 1 cut(s) | 621 |
| SfaNI | GCATC | 5 cut(s) | 203, 234, 445, 790, 1068 |
| SfcI | CTRYAG | 1 cut(s) | 89 |
| SinI | GGWCC | 3 cut(s) | 173, 236, 954 |
| SmaI | CCCGGG | 1 cut(s) | 996 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 581, 947 |
| SphI | GCATGC | 1 cut(s) | 502 |
| SseBI | AGGCCT | 1 cut(s) | 27 |
| SspMI | CTAG | 4 cut(s) | 248, 260, 444, 1034 |
| StuI | AGGCCT | 1 cut(s) | 27 |
| StyD4I | CCNGG | 5 cut(s) | 267, 517, 748, 993, 994 |
| StyI | CCWWGG | 1 cut(s) | 493 |
| TaaI | ACNGT | 2 cut(s) | 298, 308 |
| TaiI | ACGT | 1 cut(s) | 624 |
| TaqI | TCGA | 2 cut(s) | 288, 525 |
| TaqII | GACCGA | 1 cut(s) | 386 |
| TfiI | GAWTC | 1 cut(s) | 702 |
| Tru1I | TTAA | 5 cut(s) | 303, 431, 537, 624, 696 |
| Tru9I | TTAA | 5 cut(s) | 303, 431, 537, 624, 696 |
| TscAI | CASTG | 1 cut(s) | 93 |
| TseFI | GTSAC | 1 cut(s) | 367 |
| TseI | GCWGC | 3 cut(s) | 91, 628, 1079 |
| Tsp45I | GTSAC | 1 cut(s) | 367 |
| TspGWI | ACGGA | 2 cut(s) | 249, 271 |
| TspMI | CCCGGG | 1 cut(s) | 994 |
| TspRI | CASTG | 1 cut(s) | 93 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 890 |
| VpaK11BI | GGWCC | 3 cut(s) | 173, 236, 954 |
| XapI | RAATTY | 4 cut(s) | 36, 591, 1022, 1100 |
| XceI | RCATGY | 3 cut(s) | 502, 737, 778 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 964 |
| XmaI | CCCGGG | 1 cut(s) | 994 |
| XmiI | GTMKAC | 1 cut(s) | 525 |
| XmnI | GAANNNNTTC | 1 cut(s) | 786 |
| XspI | CTAG | 4 cut(s) | 248, 260, 444, 1034 |