Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 3 cut(s) | 200, 217, 395 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 890 |
| AccII | CGCG | 1 cut(s) | 1056 |
| AciI | CCGC | 1 cut(s) | 1056 |
| AclWI | GGATC | 4 cut(s) | 232, 240, 245, 850 |
| AcsI | RAATTY | 2 cut(s) | 591, 1022 |
| AcuI | CTGAAG | 1 cut(s) | 69 |
| AcyI | GRCGYC | 3 cut(s) | 32, 396, 528 |
| AdeI | CACNNNGTG | 1 cut(s) | 265 |
| AfaI | GTAC | 1 cut(s) | 359 |
| AfeI | AGCGCT | 1 cut(s) | 845 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 890, 1033 |
| AflII | CTTAAG | 1 cut(s) | 623 |
| AflIII | ACRYGT | 1 cut(s) | 774 |
| AgsI | TTSAA | 3 cut(s) | 412, 451, 550 |
| AjnI | CCWGG | 2 cut(s) | 517, 530 |
| AleI | CACNNNNGTG | 2 cut(s) | 752, 947 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 425, 457, 1008, 1040 |
| AluBI | AGCT | 5 cut(s) | 76, 124, 152, 191, 438 |
| AluI | AGCT | 5 cut(s) | 76, 124, 152, 191, 438 |
| Alw21I | GWGCWC | 3 cut(s) | 270, 621, 959 |
| Alw26I | GTCTC | 3 cut(s) | 173, 765, 929 |
| AlwI | GGATC | 4 cut(s) | 232, 240, 245, 850 |
| Ama87I | CYCGRG | 2 cut(s) | 212, 994 |
| Aor51HI | AGCGCT | 1 cut(s) | 845 |
| AoxI | GGCC | 2 cut(s) | 314, 649 |
| ApeKI | GCWGC | 4 cut(s) | 73, 188, 846, 1043 |
| ApoI | RAATTY | 2 cut(s) | 591, 1022 |
| AseI | ATTAAT | 1 cut(s) | 285 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 915, 918 |
| AspLEI | GCGC | 2 cut(s) | 398, 846 |
| AspS9I | GGNCC | 1 cut(s) | 757 |
| AsuHPI | GGTGA | 1 cut(s) | 91 |
| AvaI | CYCGRG | 2 cut(s) | 212, 994 |
| AvaII | GGWCC | 1 cut(s) | 757 |
| AxyI | CCTNAGG | 1 cut(s) | 242 |
| BamHI | GGATCC | 1 cut(s) | 237 |
| BanI | GGYRCC | 3 cut(s) | 200, 217, 395 |
| BbsI | GAAGAC | 1 cut(s) | 418 |
| Bbv12I | GWGCWC | 3 cut(s) | 270, 621, 959 |
| BbvI | GCAGC | 4 cut(s) | 85, 200, 833, 1030 |
| BccI | CCATC | 1 cut(s) | 397 |
| BciT130I | CCWGG | 2 cut(s) | 519, 532 |
| BciVI | GTATCC | 2 cut(s) | 525, 813 |
| BclI | TGATCA | 1 cut(s) | 301 |
| BcoDI | GTCTC | 3 cut(s) | 173, 765, 929 |
| BfaI | CTAG | 4 cut(s) | 251, 392, 681, 1028 |
| BfoI | RGCGCY | 2 cut(s) | 399, 847 |
| BfrI | CTTAAG | 1 cut(s) | 623 |
| BfuI | GTATCC | 2 cut(s) | 525, 813 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 404 |
| BisI | GCNGC | 5 cut(s) | 74, 189, 847, 1044, 1057 |
| BlsI | GCNGC | 5 cut(s) | 75, 190, 848, 1045, 1058 |
| BmcAI | AGTACT | 1 cut(s) | 359 |
| Bme1390I | CCNGG | 2 cut(s) | 519, 532 |
| Bme18I | GGWCC | 1 cut(s) | 757 |
| BmeT110I | CYCGRG | 2 cut(s) | 212, 994 |
| BmgT120I | GGNCC | 1 cut(s) | 757 |
| BmiI | GGNNCC | 5 cut(s) | 202, 219, 239, 397, 443 |
| BmrFI | CCNGG | 2 cut(s) | 519, 532 |
| BmsI | GCATC | 5 cut(s) | 60, 711, 724, 790, 1068 |
| BpiI | GAAGAC | 1 cut(s) | 418 |
| BpmI | CTGGAG | 2 cut(s) | 771, 853 |
| BsaBI | GATNNNNATC | 1 cut(s) | 300 |
| BsaHI | GRCGYC | 3 cut(s) | 32, 396, 528 |
| BsaI | GGTCTC | 2 cut(s) | 173, 929 |
| BsaJI | CCNNGG | 3 cut(s) | 213, 652, 1032 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 890, 1033 |
| Bse1I | ACTGG | 5 cut(s) | 445, 754, 836, 923, 949 |
| Bse21I | CCTNAGG | 1 cut(s) | 242 |
| Bse3DI | GCAATG | 2 cut(s) | 564, 988 |
| Bse8I | GATNNNNATC | 1 cut(s) | 300 |
| BseBI | CCWGG | 2 cut(s) | 519, 532 |
| BseDI | CCNNGG | 3 cut(s) | 213, 652, 1032 |
| BseGI | GGATG | 3 cut(s) | 176, 805, 976 |
| BseJI | GATNNNNATC | 1 cut(s) | 300 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 890, 1033 |
| BseMI | GCAATG | 2 cut(s) | 564, 988 |
| BseMII | CTCAG | 2 cut(s) | 206, 256 |
| BseNI | ACTGG | 5 cut(s) | 445, 754, 836, 923, 949 |
| BseRI | GAGGAG | 2 cut(s) | 163, 783 |
| BseXI | GCAGC | 4 cut(s) | 85, 200, 833, 1030 |
| BseYI | CCCAGC | 1 cut(s) | 1046 |
| Bsh1236I | CGCG | 1 cut(s) | 1056 |
| BshFI | GGCC | 2 cut(s) | 316, 651 |
| BshNI | GGYRCC | 3 cut(s) | 200, 217, 395 |
| BsiHKAI | GWGCWC | 3 cut(s) | 270, 621, 959 |
| BsiHKCI | CYCGRG | 2 cut(s) | 212, 994 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 890, 1033 |
| BsmAI | GTCTC | 3 cut(s) | 173, 765, 929 |
| BsnI | GGCC | 2 cut(s) | 316, 651 |
| Bso31I | GGTCTC | 2 cut(s) | 173, 929 |
| BsoBI | CYCGRG | 2 cut(s) | 212, 994 |
| Bsp1286I | GDGCHC | 3 cut(s) | 270, 621, 959 |
| Bsp143I | GATC | 5 cut(s) | 232, 237, 301, 598, 855 |
| BspACI | CCGC | 1 cut(s) | 1056 |
| BspANI | GGCC | 2 cut(s) | 316, 651 |
| BspCNI | CTCAG | 2 cut(s) | 205, 255 |
| BspFNI | CGCG | 1 cut(s) | 1056 |
| BspLI | GGNNCC | 5 cut(s) | 202, 219, 239, 397, 443 |
| BspPI | GGATC | 4 cut(s) | 232, 240, 245, 850 |
| BspQI | GCTCTTC | 1 cut(s) | 947 |
| BspT107I | GGYRCC | 3 cut(s) | 200, 217, 395 |
| BspTI | CTTAAG | 1 cut(s) | 623 |
| BspTNI | GGTCTC | 2 cut(s) | 173, 929 |
| BsrDI | GCAATG | 2 cut(s) | 564, 988 |
| BsrI | ACTGG | 5 cut(s) | 445, 754, 836, 923, 949 |
| BssECI | CCNNGG | 3 cut(s) | 213, 652, 1032 |
| BssMI | GATC | 5 cut(s) | 232, 237, 301, 598, 855 |
| BssNI | GRCGYC | 3 cut(s) | 32, 396, 528 |
| BssT1I | CCWWGG | 2 cut(s) | 652, 1032 |
| Bst2UI | CCWGG | 2 cut(s) | 519, 532 |
| Bst4CI | ACNGT | 3 cut(s) | 14, 280, 875 |
| Bst6I | CTCTTC | 2 cut(s) | 362, 947 |
| BstACI | GRCGYC | 3 cut(s) | 32, 396, 528 |
| BstAFI | CTTAAG | 1 cut(s) | 623 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1043 |
| BstC8I | GCNNGC | 1 cut(s) | 256 |
| BstDEI | CTNAG | 3 cut(s) | 192, 242, 665 |
| BstF5I | GGATG | 3 cut(s) | 176, 805, 976 |
| BstFNI | CGCG | 1 cut(s) | 1056 |
| BstH2I | RGCGCY | 2 cut(s) | 399, 847 |
| BstHHI | GCGC | 2 cut(s) | 398, 846 |
| BstKTI | GATC | 5 cut(s) | 235, 240, 304, 601, 858 |
| BstMAI | GTCTC | 3 cut(s) | 173, 765, 929 |
| BstMBI | GATC | 5 cut(s) | 232, 237, 301, 598, 855 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 404, 625, 1043 |
| BstNI | CCWGG | 2 cut(s) | 519, 532 |
| BstNSI | RCATGY | 1 cut(s) | 778 |
| BstSCI | CCNGG | 2 cut(s) | 517, 530 |
| BstUI | CGCG | 1 cut(s) | 1056 |
| BstV1I | GCAGC | 4 cut(s) | 85, 200, 833, 1030 |
| BstV2I | GAAGAC | 1 cut(s) | 418 |
| BstX2I | RGATCY | 1 cut(s) | 237 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 229 |
| BstYI | RGATCY | 1 cut(s) | 237 |
| Bsu36I | CCTNAGG | 1 cut(s) | 242 |
| BsuI | GTATCC | 2 cut(s) | 525, 813 |
| BsuRI | GGCC | 2 cut(s) | 316, 651 |
| BtgZI | GCGATG | 1 cut(s) | 83 |
| BtsCI | GGATG | 3 cut(s) | 176, 805, 976 |
| BtsIMutI | CAGTG | 4 cut(s) | 276, 747, 880, 942 |
| Cac8I | GCNNGC | 1 cut(s) | 256 |
| CfoI | GCGC | 2 cut(s) | 398, 846 |
| Cfr13I | GGNCC | 1 cut(s) | 757 |
| CseI | GACGC | 2 cut(s) | 21, 536 |
| Csp6I | GTAC | 1 cut(s) | 358 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 136, 171 |
| CviAII | CATG | 7 cut(s) | 100, 106, 223, 347, 722, 761, 775 |
| CviQI | GTAC | 1 cut(s) | 358 |
| DdeI | CTNAG | 3 cut(s) | 192, 242, 665 |
| DinI | GGCGCC | 1 cut(s) | 397 |
| DpnI | GATC | 5 cut(s) | 234, 239, 303, 600, 857 |
| DpnII | GATC | 5 cut(s) | 232, 237, 301, 598, 855 |
| DraIII | CACNNNGTG | 1 cut(s) | 265 |
| Eam1104I | CTCTTC | 2 cut(s) | 362, 947 |
| EarI | CTCTTC | 2 cut(s) | 362, 947 |
| Eco130I | CCWWGG | 2 cut(s) | 652, 1032 |
| Eco147I | AGGCCT | 1 cut(s) | 316 |
| Eco31I | GGTCTC | 2 cut(s) | 173, 929 |
| Eco32I | GATATC | 1 cut(s) | 686 |
| Eco47I | GGWCC | 1 cut(s) | 757 |
| Eco47III | AGCGCT | 1 cut(s) | 845 |
| Eco57I | CTGAAG | 1 cut(s) | 69 |
| Eco81I | CCTNAGG | 1 cut(s) | 242 |
| Eco88I | CYCGRG | 2 cut(s) | 212, 994 |
| EcoRI | GAATTC | 1 cut(s) | 1022 |
| EcoRII | CCWGG | 2 cut(s) | 517, 530 |
| EcoRV | GATATC | 1 cut(s) | 686 |
| EcoT14I | CCWWGG | 2 cut(s) | 652, 1032 |
| EgeI | GGCGCC | 1 cut(s) | 397 |
| EheI | GGCGCC | 1 cut(s) | 397 |
| ErhI | CCWWGG | 2 cut(s) | 652, 1032 |
| FaeI | CATG | 7 cut(s) | 103, 109, 226, 350, 725, 764, 778 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 413, 445, 903, 935 |
| FatI | CATG | 7 cut(s) | 99, 105, 222, 346, 721, 760, 774 |
| FbaI | TGATCA | 1 cut(s) | 301 |
| Fnu4HI | GCNGC | 5 cut(s) | 74, 189, 847, 1044, 1057 |
| FokI | GGATG | 3 cut(s) | 163, 812, 983 |
| Fsp4HI | GCNGC | 5 cut(s) | 74, 189, 847, 1044, 1057 |
| FspBI | CTAG | 4 cut(s) | 251, 392, 681, 1028 |
| GlaI | GCGC | 2 cut(s) | 397, 845 |
| GluI | GCNGC | 5 cut(s) | 74, 189, 847, 1044, 1057 |
| GsaI | CCCAGC | 1 cut(s) | 1050 |
| GsuI | CTGGAG | 2 cut(s) | 771, 853 |
| HaeII | RGCGCY | 2 cut(s) | 399, 847 |
| HaeIII | GGCC | 2 cut(s) | 316, 651 |
| HgaI | GACGC | 2 cut(s) | 21, 536 |
| HhaI | GCGC | 2 cut(s) | 398, 846 |
| Hin1I | GRCGYC | 3 cut(s) | 32, 396, 528 |
| Hin1II | CATG | 7 cut(s) | 103, 109, 226, 350, 725, 764, 778 |
| Hin6I | GCGC | 2 cut(s) | 396, 844 |
| HinP1I | GCGC | 2 cut(s) | 396, 844 |
| HinfI | GANTC | 3 cut(s) | 412, 474, 661 |
| HphI | GGTGA | 1 cut(s) | 91 |
| Hpy166II | GTNNAC | 3 cut(s) | 36, 757, 1016 |
| Hpy188I | TCNGA | 3 cut(s) | 195, 237, 1064 |
| Hpy188III | TCNNGA | 6 cut(s) | 416, 586, 596, 681, 689, 859 |
| Hpy8I | GTNNAC | 3 cut(s) | 36, 757, 1016 |
| HpyAV | CCTTC | 2 cut(s) | 461, 750 |
| HpyCH4III | ACNGT | 3 cut(s) | 14, 280, 875 |
| HpyCH4V | TGCA | 3 cut(s) | 73, 188, 276 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 404, 625, 1043 |
| HpyF3I | CTNAG | 3 cut(s) | 192, 242, 665 |
| Hsp92I | GRCGYC | 3 cut(s) | 32, 396, 528 |
| Hsp92II | CATG | 7 cut(s) | 103, 109, 226, 350, 725, 764, 778 |
| HspAI | GCGC | 2 cut(s) | 396, 844 |
| KasI | GGCGCC | 1 cut(s) | 395 |
| Ksp22I | TGATCA | 1 cut(s) | 301 |
| Kzo9I | GATC | 5 cut(s) | 232, 237, 301, 598, 855 |
| LguI | GCTCTTC | 1 cut(s) | 947 |
| Lsp1109I | GCAGC | 4 cut(s) | 85, 200, 833, 1030 |
| LweI | GCATC | 5 cut(s) | 60, 711, 724, 790, 1068 |
| MaeI | CTAG | 4 cut(s) | 251, 392, 681, 1028 |
| MaeIII | GTNAC | 3 cut(s) | 79, 658, 869 |
| MalI | GATC | 5 cut(s) | 234, 239, 303, 600, 857 |
| MboI | GATC | 5 cut(s) | 232, 237, 301, 598, 855 |
| MboII | GAAGA | 8 cut(s) | 56, 59, 62, 379, 418, 911, 923, 964 |
| MfeI | CAATTG | 3 cut(s) | 271, 542, 1038 |
| MflI | RGATCY | 1 cut(s) | 237 |
| MhlI | GDGCHC | 3 cut(s) | 270, 621, 959 |
| Mly113I | GGCGCC | 1 cut(s) | 396 |
| MlyI | GAGTC | 1 cut(s) | 655 |
| MmeI | TCCRAC | 1 cut(s) | 210 |
| MnlI | CCTC | 8 cut(s) | 184, 208, 251, 464, 729, 761, 970, 992 |
| MroXI | GAANNNNTTC | 2 cut(s) | 915, 918 |
| MseI | TTAA | 6 cut(s) | 285, 483, 537, 624, 696, 1107 |
| MslI | CAYNNNNRTG | 5 cut(s) | 104, 557, 752, 809, 947 |
| MspA1I | CMGCKG | 2 cut(s) | 76, 438 |
| MspCI | CTTAAG | 1 cut(s) | 623 |
| MspR9I | CCNGG | 2 cut(s) | 519, 532 |
| MunI | CAATTG | 3 cut(s) | 271, 542, 1038 |
| MvaI | CCWGG | 2 cut(s) | 519, 532 |
| MvnI | CGCG | 1 cut(s) | 1056 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 404, 625, 1043 |
| NarI | GGCGCC | 1 cut(s) | 396 |
| NdeII | GATC | 5 cut(s) | 232, 237, 301, 598, 855 |
| NlaIII | CATG | 7 cut(s) | 103, 109, 226, 350, 725, 764, 778 |
| NlaIV | GGNNCC | 5 cut(s) | 202, 219, 239, 397, 443 |
| NmuCI | GTSAC | 2 cut(s) | 79, 658 |
| NspI | RCATGY | 1 cut(s) | 778 |
| OliI | CACNNNNGTG | 2 cut(s) | 752, 947 |
| PceI | AGGCCT | 1 cut(s) | 316 |
| PciI | ACATGT | 1 cut(s) | 774 |
| PciSI | GCTCTTC | 1 cut(s) | 947 |
| PdmI | GAANNNNTTC | 2 cut(s) | 915, 918 |
| PfeI | GAWTC | 2 cut(s) | 412, 474 |
| PflFI | GACNNNGTC | 1 cut(s) | 422 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 890 |
| PkrI | GCNGC | 5 cut(s) | 75, 190, 848, 1045, 1058 |
| PleI | GAGTC | 1 cut(s) | 655 |
| PluTI | GGCGCC | 1 cut(s) | 399 |
| PpsI | GAGTC | 1 cut(s) | 655 |
| PscI | ACATGT | 1 cut(s) | 774 |
| PshBI | ATTAAT | 1 cut(s) | 285 |
| Psp6I | CCWGG | 2 cut(s) | 517, 530 |
| PspFI | CCCAGC | 1 cut(s) | 1046 |
| PspGI | CCWGG | 2 cut(s) | 517, 530 |
| PspN4I | GGNNCC | 5 cut(s) | 202, 219, 239, 397, 443 |
| PspPI | GGNCC | 1 cut(s) | 757 |
| PsuI | RGATCY | 1 cut(s) | 237 |
| PsyI | GACNNNGTC | 1 cut(s) | 422 |
| PvuII | CAGCTG | 2 cut(s) | 76, 438 |
| RsaI | GTAC | 1 cut(s) | 359 |
| RsaNI | GTAC | 1 cut(s) | 358 |
| RseI | CAYNNNNRTG | 5 cut(s) | 104, 557, 752, 809, 947 |
| SapI | GCTCTTC | 1 cut(s) | 947 |
| SaqAI | TTAA | 6 cut(s) | 285, 483, 537, 624, 696, 1107 |
| SatI | GCNGC | 5 cut(s) | 74, 189, 847, 1044, 1057 |
| Sau3AI | GATC | 5 cut(s) | 232, 237, 301, 598, 855 |
| Sau96I | GGNCC | 1 cut(s) | 757 |
| ScaI | AGTACT | 1 cut(s) | 359 |
| SchI | GAGTC | 1 cut(s) | 655 |
| ScrFI | CCNGG | 2 cut(s) | 519, 532 |
| SduI | GDGCHC | 3 cut(s) | 270, 621, 959 |
| SfaNI | GCATC | 5 cut(s) | 60, 711, 724, 790, 1068 |
| SfoI | GGCGCC | 1 cut(s) | 397 |
| SinI | GGWCC | 1 cut(s) | 757 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 104, 557, 752, 809, 947 |
| SmlI | CTYRAG | 1 cut(s) | 623 |
| SmoI | CTYRAG | 1 cut(s) | 623 |
| SseBI | AGGCCT | 1 cut(s) | 316 |
| SsiI | CCGC | 1 cut(s) | 1056 |
| SspDI | GGCGCC | 1 cut(s) | 395 |
| SspMI | CTAG | 4 cut(s) | 251, 392, 681, 1028 |
| StuI | AGGCCT | 1 cut(s) | 316 |
| StyD4I | CCNGG | 2 cut(s) | 517, 530 |
| StyI | CCWWGG | 2 cut(s) | 652, 1032 |
| TaaI | ACNGT | 3 cut(s) | 14, 280, 875 |
| TaqI | TCGA | 2 cut(s) | 135, 564 |
| TatI | WGTACW | 1 cut(s) | 357 |
| TauI | GCSGC | 1 cut(s) | 1059 |
| TfiI | GAWTC | 2 cut(s) | 412, 474 |
| Tru1I | TTAA | 6 cut(s) | 285, 483, 537, 624, 696, 1107 |
| Tru9I | TTAA | 6 cut(s) | 285, 483, 537, 624, 696, 1107 |
| TscAI | CASTG | 4 cut(s) | 283, 754, 880, 949 |
| TseFI | GTSAC | 2 cut(s) | 79, 658 |
| TseI | GCWGC | 4 cut(s) | 73, 188, 846, 1043 |
| Tsp45I | GTSAC | 2 cut(s) | 79, 658 |
| TspDTI | ATGAA | 6 cut(s) | 122, 314, 390, 815, 827, 1083 |
| TspRI | CASTG | 4 cut(s) | 283, 754, 880, 949 |
| Tth111I | GACNNNGTC | 1 cut(s) | 422 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 890 |
| Vha464I | CTTAAG | 1 cut(s) | 623 |
| VpaK11BI | GGWCC | 1 cut(s) | 757 |
| VspI | ATTAAT | 1 cut(s) | 285 |
| XapI | RAATTY | 2 cut(s) | 591, 1022 |
| XbaI | TCTAGA | 1 cut(s) | 680 |
| XceI | RCATGY | 1 cut(s) | 778 |
| XmnI | GAANNNNTTC | 2 cut(s) | 915, 918 |
| XspI | CTAG | 4 cut(s) | 251, 392, 681, 1028 |
| ZrmI | AGTACT | 1 cut(s) | 359 |