Rh6CG295900
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000357
Physical Location & Seq
Reverse (-)
47681330 .. 47683736
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG295900.1

Sequence Viewer

Length: 783 bp
ATGAGCATTGATAGTTCGAAGCCAGTAATGACGTCCACAAAAGAAGAAGAAGAAAGCTTCAGCTCTGCAATGCAGCTGGTGACTTCTACTGTGCTGTCTATGTCCATGCAATCAGCAGTTGAGCTTGGAGTTTTCGACATCATAGCCAAAGCTGGTGCGGGTGCGGGTCTCTCCTCAACCAAGATTGTAGCTCAGCTAGGCACCCAGAACTCCGAGGCGCCCAAGATGTTGGATCGAATCCTCAGGCTACTGGCAAGTCACTCTGTGCTCAACTGCACCGTGGTTAATGCTAATGATGATGATCAGAGTGATGGGCCTAATTTTCAGAGGGTTTATAGTCTTGCTCCTGTGTCCAAGTACTTCGTGAAAAGTGATGAAGACGGTGTTTCTTTAGGCGCCCTGATGGCATTGGTTCAAGACAAGGTCTACTTGGACAGCTGGTTCCAGTTGAAAGGTGCTGTTGTAGAAGGAGGAATCCCATTTAACAGGGTCCATGGCACACATGCCTTTGAGTATCCAGGTTTGGATCCCAGGTTTAACCAAGTGTTCAACACAGCAATGTTCAACCACACCACTATTGTTATCAAGAAAATTCTTGATCTCTACAAGGGTTTTGAGCACCTTAAGCAACTTGTTGATGTTGGGGGTGGCCTTGGAGTGACTCTTAGCCTAATCACTTCTAGATACCCTCACATTAAGGGCATCAACTTTGACTTGCCTCATGTCATCAAAGATGCCCCTTCTTACACTGGTATGATCATGACCTTCAGTATTTTACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01058 ko01100 ko01110 map00940 map01058 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

260

Amino Acids

28.28

Weight (kDa)

5.78

Isoelectric Point (pI)

30.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 24 - 124 9.8e-20 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 147 - 249 9.8e-28 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000357)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17810 FvH4_7g17811 FvH4_7g17820 FvH4_7g17830
malus_domestica MD00G1100700.v1.1 MD01G1089600.v1.1 MD01G1089800.v1.1 MD01G1090400.v1.1 MD01G1090500.v1.1 MD01G1090800.v1.1 MD04G1141100.v1.1 MD07G1161000.v1.1 MD07G1161100.v1.1 MD10G1030000.v1.1 MD10G1030200.v1.1 MD15G1409200.v1.1 MD15G1409700.v1.1
prunus_persica Prupe.2G199100_v2.0.a1 Prupe.2G199100_v2.0.a1 Prupe.2G199300_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199500_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199800_v2.0.a1 Prupe.2G200100_v2.0.a1 Prupe.2G200100_v2.0.a1
pyrus_communis pycom01g11360 pycom01g11370 pycom01g11400 pycom01g11440 pycom01g11480 pycom01g11490 pycom07g15690 pycom08g12100
rosa_chinensis RchiOBHm_Chr1g0317931 RchiOBHm_Chr1g0361211 RchiOBHm_Chr1g0361221 RchiOBHm_Chr1g0361241 RchiOBHm_Chr1g0367691 RchiOBHm_Chr4g0390171 RchiOBHm_Chr4g0396271 RchiOBHm_Chr6g0286421
rosa_laevigata RLG00000002555 RLG00000009555 RLG00000027275 RLG00000027731 RLG00000027732 RLG00000027734 RLG00000027738 RLG00000030575
rosa_multiflora Rmu_co8470451.1_g000001 Rmu_sc0000250.1_g000010 Rmu_sc0010379.1_g000006 Rmu_sc0011132.1_g000005 Rmu_sc0011132.1_g000007 Rmu_sc0038028.1_g000001 Rmu_ssc0000008.1_g000043 Rmu_ssc0000234.1_g000002 Rmu_ssc0000418.1_g000001
rosa_roxburghii Rroxscaffold_2G00116370 Rroxscaffold_4G00289300 Rroxscaffold_4G00295130 Rroxscaffold_4G00295140 Rroxscaffold_4G00295160 Rroxscaffold_4G00295190 Rroxscaffold_4G00295220 Rroxscaffold_4G00330100 Rroxscaffold_5G00341100
rosa_rugosa Rorug01G0287600 Rorug01G0287700 Rorug01G0336200 Rorug01G0336200 Rorug03G0350700
rosa_samantha Rh1AG030300 Rh1AG298500 Rh1AG298600 Rh1AG298700 Rh1AG298900 Rh1BG262400 Rh1BG262500 Rh1BG262800 Rh1BG304800 Rh1CG029000 Rh1CG279800 Rh1CG279900 Rh1CG280000 Rh1CG280100 Rh1CG280200 Rh1CG320500 Rh1DG041200 Rh1DG292700 Rh1DG292800 Rh1DG337100 Rh3DG107500 Rh4AG128100 Rh4BG024900 Rh4CG072700 Rh4DG063000 Rh6CG295900 Rh6DG287700
rosa_wichuraiana Rw1G002290 Rw1G026410 Rw1G026420 Rw1G026440 Rw4G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 35
AccB1I GGYRCC 3 cut(s) 200, 217, 395
AccI GTMKAC 1 cut(s) 426
AciI CCGC 2 cut(s) 158, 164
AclWI GGATC 3 cut(s) 240, 521, 534
AcsI RAATTY 1 cut(s) 591
AcuI CTGAAG 2 cut(s) 43, 751
AcyI GRCGYC 3 cut(s) 32, 218, 396
AdeI CACNNNGTG 1 cut(s) 265
AfaI GTAC 1 cut(s) 359
AflII CTTAAG 1 cut(s) 623
AgsI TTSAA 4 cut(s) 416, 451, 550, 565
AjnI CCWGG 2 cut(s) 517, 530
AloI GAACNNNNNNTCC 2 cut(s) 425, 457
AluBI AGCT 8 cut(s) 57, 63, 76, 124, 152, 191, 196, 438
AluI AGCT 8 cut(s) 57, 63, 76, 124, 152, 191, 196, 438
Alw21I GWGCWC 2 cut(s) 270, 621
Alw26I GTCTC 1 cut(s) 173
AlwI GGATC 3 cut(s) 240, 521, 534
AoxI GGCC 2 cut(s) 314, 649
ApeKI GCWGC 1 cut(s) 73
ApoI RAATTY 1 cut(s) 591
AspLEI GCGC 2 cut(s) 220, 398
AspS9I GGNCC 2 cut(s) 314, 490
AsuHPI GGTGA 1 cut(s) 91
AsuII TTCGAA 1 cut(s) 17
AvaII GGWCC 1 cut(s) 490
AxyI CCTNAGG 1 cut(s) 242
BamHI GGATCC 1 cut(s) 526
BanI GGYRCC 3 cut(s) 200, 217, 395
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 2 cut(s) 270, 621
BbvI GCAGC 1 cut(s) 85
BccI CCATC 2 cut(s) 305, 397
BciT130I CCWGG 2 cut(s) 519, 532
BciVI GTATCC 1 cut(s) 525
BclI TGATCA 2 cut(s) 301, 756
BcoDI GTCTC 1 cut(s) 173
BfaI CTAG 2 cut(s) 197, 681
BfoI RGCGCY 2 cut(s) 221, 399
BfrI CTTAAG 1 cut(s) 623
BfuI GTATCC 1 cut(s) 525
BglI GCCNNNNNGGC 1 cut(s) 404
BisI GCNGC 1 cut(s) 74
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 75
BmcAI AGTACT 1 cut(s) 359
Bme1390I CCNGG 2 cut(s) 519, 532
Bme18I GGWCC 1 cut(s) 490
BmgT120I GGNCC 2 cut(s) 314, 490
BmiI GGNNCC 6 cut(s) 202, 219, 397, 443, 491, 528
BmrFI CCNGG 2 cut(s) 519, 532
BmsI GCATC 2 cut(s) 711, 724
BpiI GAAGAC 1 cut(s) 384
Bpu1102I GCTNAGC 1 cut(s) 192
Bpu14I TTCGAA 1 cut(s) 17
BsaBI GATNNNNATC 1 cut(s) 300
BsaHI GRCGYC 3 cut(s) 32, 218, 396
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 5 cut(s) 213, 279, 493, 530, 652
Bse1I ACTGG 4 cut(s) 23, 255, 445, 754
Bse21I CCTNAGG 1 cut(s) 242
Bse3DI GCAATG 2 cut(s) 75, 564
Bse8I GATNNNNATC 1 cut(s) 300
BseBI CCWGG 2 cut(s) 519, 532
BseDI CCNNGG 5 cut(s) 213, 279, 493, 530, 652
BseJI GATNNNNATC 1 cut(s) 300
BseMI GCAATG 2 cut(s) 75, 564
BseMII CTCAG 2 cut(s) 206, 256
BseNI ACTGG 4 cut(s) 23, 255, 445, 754
BseRI GAGGAG 1 cut(s) 163
BseXI GCAGC 1 cut(s) 85
BsgI GTGCAG 1 cut(s) 259
BshFI GGCC 2 cut(s) 316, 651
BshNI GGYRCC 3 cut(s) 200, 217, 395
BsiHKAI GWGCWC 2 cut(s) 270, 621
BsmAI GTCTC 1 cut(s) 173
BsnI GGCC 2 cut(s) 316, 651
Bso31I GGTCTC 1 cut(s) 173
Bsp119I TTCGAA 1 cut(s) 17
Bsp1286I GDGCHC 2 cut(s) 270, 621
Bsp143I GATC 5 cut(s) 232, 301, 526, 598, 756
Bsp1720I GCTNAGC 1 cut(s) 192
Bsp19I CCATGG 1 cut(s) 493
BspACI CCGC 2 cut(s) 158, 164
BspANI GGCC 2 cut(s) 316, 651
BspCNI CTCAG 2 cut(s) 205, 255
BspHI TCATGA 1 cut(s) 759
BspLI GGNNCC 6 cut(s) 202, 219, 397, 443, 491, 528
BspPI GGATC 3 cut(s) 240, 521, 534
BspT104I TTCGAA 1 cut(s) 17
BspT107I GGYRCC 3 cut(s) 200, 217, 395
BspTI CTTAAG 1 cut(s) 623
BspTNI GGTCTC 1 cut(s) 173
BsrDI GCAATG 2 cut(s) 75, 564
BsrI ACTGG 4 cut(s) 23, 255, 445, 754
BssECI CCNNGG 5 cut(s) 213, 279, 493, 530, 652
BssMI GATC 5 cut(s) 232, 301, 526, 598, 756
BssNI GRCGYC 3 cut(s) 32, 218, 396
BssT1I CCWWGG 2 cut(s) 493, 652
Bst2UI CCWGG 2 cut(s) 519, 532
Bst4CI ACNGT 4 cut(s) 91, 280, 383, 780
BstACI GRCGYC 3 cut(s) 32, 218, 396
BstAFI CTTAAG 1 cut(s) 623
BstBI TTCGAA 1 cut(s) 17
BstDEI CTNAG 3 cut(s) 192, 242, 665
BstDSI CCRYGG 2 cut(s) 279, 493
BstH2I RGCGCY 2 cut(s) 221, 399
BstHHI GCGC 2 cut(s) 220, 398
BstKTI GATC 5 cut(s) 235, 304, 529, 601, 759
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 5 cut(s) 232, 301, 526, 598, 756
BstMWI GCNNNNNNNGC 2 cut(s) 404, 625
BstNI CCWGG 2 cut(s) 519, 532
BstNSI RCATGY 1 cut(s) 506
BstSCI CCNGG 2 cut(s) 517, 530
BstV1I GCAGC 1 cut(s) 85
BstV2I GAAGAC 1 cut(s) 384
BstX2I RGATCY 1 cut(s) 526
BstXI CCANNNNNNTGG 1 cut(s) 229
BstYI RGATCY 1 cut(s) 526
Bsu36I CCTNAGG 1 cut(s) 242
BsuI GTATCC 1 cut(s) 525
BsuRI GGCC 2 cut(s) 316, 651
BtgI CCRYGG 2 cut(s) 279, 493
BtsIMutI CAGTG 1 cut(s) 747
CciI TCATGA 1 cut(s) 759
CfoI GCGC 2 cut(s) 220, 398
Cfr13I GGNCC 2 cut(s) 314, 490
Csp6I GTAC 1 cut(s) 358
CviAII CATG 5 cut(s) 106, 494, 503, 722, 760
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 3 cut(s) 192, 242, 665
DinI GGCGCC 2 cut(s) 219, 397
DpnI GATC 5 cut(s) 234, 303, 528, 600, 758
DpnII GATC 5 cut(s) 232, 301, 526, 598, 756
DraIII CACNNNGTG 1 cut(s) 265
Eco130I CCWWGG 2 cut(s) 493, 652
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 1 cut(s) 490
Eco57I CTGAAG 2 cut(s) 43, 751
Eco81I CCTNAGG 1 cut(s) 242
EcoRII CCWGG 2 cut(s) 517, 530
EcoT14I CCWWGG 2 cut(s) 493, 652
EgeI GGCGCC 2 cut(s) 219, 397
EheI GGCGCC 2 cut(s) 219, 397
ErhI CCWWGG 2 cut(s) 493, 652
FaeI CATG 5 cut(s) 109, 497, 506, 725, 763
FaiI YATR 9 cut(s) 101, 107, 143, 336, 495, 504, 723, 755, 761
FalI AAGNNNNNCTT 2 cut(s) 413, 445
FatI CATG 5 cut(s) 105, 493, 502, 721, 759
FauI CCCGC 2 cut(s) 151, 157
FbaI TGATCA 2 cut(s) 301, 756
FblI GTMKAC 1 cut(s) 426
Fnu4HI GCNGC 1 cut(s) 74
Fsp4HI GCNGC 1 cut(s) 74
FspBI CTAG 2 cut(s) 197, 681
GlaI GCGC 2 cut(s) 219, 397
GluI GCNGC 1 cut(s) 74
HaeII RGCGCY 2 cut(s) 221, 399
HaeIII GGCC 2 cut(s) 316, 651
HhaI GCGC 2 cut(s) 220, 398
Hin1I GRCGYC 3 cut(s) 32, 218, 396
Hin1II CATG 5 cut(s) 109, 497, 506, 725, 763
Hin6I GCGC 2 cut(s) 218, 396
HinP1I GCGC 2 cut(s) 218, 396
HindIII AAGCTT 1 cut(s) 55
HinfI GANTC 3 cut(s) 237, 474, 661
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 2 cut(s) 36, 427
Hpy188I TCNGA 3 cut(s) 214, 306, 327
Hpy188III TCNNGA 6 cut(s) 364, 416, 586, 596, 681, 760
Hpy8I GTNNAC 2 cut(s) 36, 427
HpyAV CCTTC 3 cut(s) 461, 750, 775
HpyCH4III ACNGT 4 cut(s) 91, 280, 383, 780
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 4 cut(s) 68, 73, 109, 276
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 625
HpyF3I CTNAG 3 cut(s) 192, 242, 665
HpySE526I ACGT 1 cut(s) 32
Hsp92I GRCGYC 3 cut(s) 32, 218, 396
Hsp92II CATG 5 cut(s) 109, 497, 506, 725, 763
HspAI GCGC 2 cut(s) 218, 396
KasI GGCGCC 2 cut(s) 217, 395
Ksp22I TGATCA 2 cut(s) 301, 756
Kzo9I GATC 5 cut(s) 232, 301, 526, 598, 756
LmnI GCTCC 1 cut(s) 349
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 2 cut(s) 711, 724
MaeI CTAG 2 cut(s) 197, 681
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 3 cut(s) 79, 257, 658
MalI GATC 5 cut(s) 234, 303, 528, 600, 758
MboI GATC 5 cut(s) 232, 301, 526, 598, 756
MboII GAAGA 4 cut(s) 56, 59, 62, 389
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 2 cut(s) 270, 621
MluCI AATT 2 cut(s) 319, 591
Mly113I GGCGCC 2 cut(s) 218, 396
MlyI GAGTC 1 cut(s) 655
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 7 cut(s) 184, 208, 251, 321, 464, 699, 729
MseI TTAA 5 cut(s) 285, 483, 537, 624, 696
MslI CAYNNNNRTG 2 cut(s) 557, 752
MspA1I CMGCKG 2 cut(s) 76, 438
MspCI CTTAAG 1 cut(s) 623
MspR9I CCNGG 2 cut(s) 519, 532
MvaI CCWGG 2 cut(s) 519, 532
MwoI GCNNNNNNNGC 2 cut(s) 404, 625
NarI GGCGCC 2 cut(s) 218, 396
NcoI CCATGG 1 cut(s) 493
NdeII GATC 5 cut(s) 232, 301, 526, 598, 756
NlaIII CATG 5 cut(s) 109, 497, 506, 725, 763
NlaIV GGNNCC 6 cut(s) 202, 219, 397, 443, 491, 528
NmuCI GTSAC 3 cut(s) 79, 257, 658
NspI RCATGY 1 cut(s) 506
NspV TTCGAA 1 cut(s) 17
PagI TCATGA 1 cut(s) 759
PfeI GAWTC 2 cut(s) 237, 474
PflFI GACNNNGTC 1 cut(s) 422
PkrI GCNGC 1 cut(s) 75
PleI GAGTC 1 cut(s) 655
PluTI GGCGCC 2 cut(s) 221, 399
PpsI GAGTC 1 cut(s) 655
Psp6I CCWGG 2 cut(s) 517, 530
PspGI CCWGG 2 cut(s) 517, 530
PspN4I GGNNCC 6 cut(s) 202, 219, 397, 443, 491, 528
PspPI GGNCC 2 cut(s) 314, 490
PsuI RGATCY 1 cut(s) 526
PsyI GACNNNGTC 1 cut(s) 422
PvuII CAGCTG 2 cut(s) 76, 438
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
RseI CAYNNNNRTG 2 cut(s) 557, 752
SaqAI TTAA 5 cut(s) 285, 483, 537, 624, 696
SatI GCNGC 1 cut(s) 74
Sau3AI GATC 5 cut(s) 232, 301, 526, 598, 756
Sau96I GGNCC 2 cut(s) 314, 490
ScaI AGTACT 1 cut(s) 359
SchI GAGTC 1 cut(s) 655
ScrFI CCNGG 2 cut(s) 519, 532
SduI GDGCHC 2 cut(s) 270, 621
SfaNI GCATC 2 cut(s) 711, 724
SfoI GGCGCC 2 cut(s) 219, 397
SfuI TTCGAA 1 cut(s) 17
SinI GGWCC 1 cut(s) 490
SmiMI CAYNNNNRTG 2 cut(s) 557, 752
SmlI CTYRAG 1 cut(s) 623
SmoI CTYRAG 1 cut(s) 623
Sse9I AATT 2 cut(s) 319, 591
SsiI CCGC 2 cut(s) 158, 164
SspDI GGCGCC 2 cut(s) 217, 395
SspMI CTAG 2 cut(s) 197, 681
StyD4I CCNGG 2 cut(s) 517, 530
StyI CCWWGG 2 cut(s) 493, 652
TaaI ACNGT 4 cut(s) 91, 280, 383, 780
TaiI ACGT 1 cut(s) 35
TaqI TCGA 3 cut(s) 17, 135, 235
TasI AATT 2 cut(s) 319, 591
TatI WGTACW 1 cut(s) 357
TfiI GAWTC 2 cut(s) 237, 474
Tru1I TTAA 5 cut(s) 285, 483, 537, 624, 696
Tru9I TTAA 5 cut(s) 285, 483, 537, 624, 696
TscAI CASTG 1 cut(s) 754
TseFI GTSAC 3 cut(s) 79, 257, 658
TseI GCWGC 1 cut(s) 73
Tsp45I GTSAC 3 cut(s) 79, 257, 658
TspDTI ATGAA 1 cut(s) 390
TspRI CASTG 1 cut(s) 754
Tth111I GACNNNGTC 1 cut(s) 422
Vha464I CTTAAG 1 cut(s) 623
VpaK11BI GGWCC 1 cut(s) 490
XapI RAATTY 1 cut(s) 591
XbaI TCTAGA 1 cut(s) 680
XceI RCATGY 1 cut(s) 506
XmiI GTMKAC 1 cut(s) 426
XspI CTAG 2 cut(s) 197, 681
ZraI GACGTC 1 cut(s) 33
ZrmI AGTACT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.