MD01G1090800.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000357
Physical Location & Seq
Forward (+)
20555929 .. 20558267
2339 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1090800.v1.1.491

Sequence Viewer

Length: 828 bp
ATGTTGAAACACATACTAAGTGGCATTAAATATCACAATACAAATATAGACGGTCCAATGGCCCTGCAGCTAGAAGAAGAAGAGAACTTCTGCTATGCCATTGAGCTGGTGTCTTCTTCTGTGCCTTCCATGTCTATGCAATCAGCAATCGAGCTAGGTGTTTTCGACATCATAGCAAAAGCCGGTCCTGGTGCCAAGCTCTCTTCATCAGAGATTGCAGCCCATATCGGCAGTGGCACCAGGAATTCTGAGGGACCAATGATGTTGGATCGTATTCTGAGGCTTCTAGCCAAAGATGGGCCTGCTTCTCAGAGGCTCTACAGCCTTGTCCCTGTGTCCAACTACGTTGTGACTAATGAAGATGGTGTTTCTTTAGTTCCCTTGATGGCGTTGATGCAAGACAAGGTCCTCCTAGACAGCTGGTCCCAACTGAAAGATGCAGTTGTTGAAAGAGGAATTCCATTTAACAGAGTCCACGGCACTCATGCTTTTGAGTATGCAGGTTTAGACCCCAGGTTTAATCAAGTTTTCAACACAGCAATGTTTAACCACGCCACTATTGTCATGAAGAAGATTCTTCAACTCTACAAGGGTTTTGAGAAACTTACCCAACTTGTTGATGTTGGTGGTGGTTTGGGAGTCACTCTTAGTCTAATCACTTCTAAACATCGTCATATTAAGGGGGTGGAACATGTTGGAGGAGACATGTTTGCAAGTGTTCCATCTGGGGATGCCATTTTTAAGAAGTTAATACTTCACGATTGGAGTGACCAGCACTGCCTAAATCTGTTGAAAATTGTTACAATGCTATACCAGACCGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01058 ko01100 ko01110 map00940 map01058 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

276

Amino Acids

30.18

Weight (kDa)

6.59

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 35 - 118 8.1e-13 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 141 - 228 1.1e-18 O-methyltransferase domain
Methyltransf_2 PF00891 227 - 268 2.4e-11 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000357)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17810 FvH4_7g17811 FvH4_7g17820 FvH4_7g17830
malus_domestica MD00G1100700.v1.1 MD01G1089600.v1.1 MD01G1089800.v1.1 MD01G1090400.v1.1 MD01G1090500.v1.1 MD01G1090800.v1.1 MD04G1141100.v1.1 MD07G1161000.v1.1 MD07G1161100.v1.1 MD10G1030000.v1.1 MD10G1030200.v1.1 MD15G1409200.v1.1 MD15G1409700.v1.1
prunus_persica Prupe.2G199100_v2.0.a1 Prupe.2G199100_v2.0.a1 Prupe.2G199300_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199500_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199800_v2.0.a1 Prupe.2G200100_v2.0.a1 Prupe.2G200100_v2.0.a1
pyrus_communis pycom01g11360 pycom01g11370 pycom01g11400 pycom01g11440 pycom01g11480 pycom01g11490 pycom07g15690 pycom08g12100
rosa_chinensis RchiOBHm_Chr1g0317931 RchiOBHm_Chr1g0361211 RchiOBHm_Chr1g0361221 RchiOBHm_Chr1g0361241 RchiOBHm_Chr1g0367691 RchiOBHm_Chr4g0390171 RchiOBHm_Chr4g0396271 RchiOBHm_Chr6g0286421
rosa_laevigata RLG00000002555 RLG00000009555 RLG00000027275 RLG00000027731 RLG00000027732 RLG00000027734 RLG00000027738 RLG00000030575
rosa_multiflora Rmu_co8470451.1_g000001 Rmu_sc0000250.1_g000010 Rmu_sc0010379.1_g000006 Rmu_sc0011132.1_g000005 Rmu_sc0011132.1_g000007 Rmu_sc0038028.1_g000001 Rmu_ssc0000008.1_g000043 Rmu_ssc0000234.1_g000002 Rmu_ssc0000418.1_g000001
rosa_roxburghii Rroxscaffold_2G00116370 Rroxscaffold_4G00289300 Rroxscaffold_4G00295130 Rroxscaffold_4G00295140 Rroxscaffold_4G00295160 Rroxscaffold_4G00295190 Rroxscaffold_4G00295220 Rroxscaffold_4G00330100 Rroxscaffold_5G00341100
rosa_rugosa Rorug01G0287600 Rorug01G0287700 Rorug01G0336200 Rorug01G0336200 Rorug03G0350700
rosa_samantha Rh1AG030300 Rh1AG298500 Rh1AG298600 Rh1AG298700 Rh1AG298900 Rh1BG262400 Rh1BG262500 Rh1BG262800 Rh1BG304800 Rh1CG029000 Rh1CG279800 Rh1CG279900 Rh1CG280000 Rh1CG280100 Rh1CG280200 Rh1CG320500 Rh1DG041200 Rh1DG292700 Rh1DG292800 Rh1DG337100 Rh3DG107500 Rh4AG128100 Rh4BG024900 Rh4CG072700 Rh4DG063000 Rh6CG295900 Rh6DG287700
rosa_wichuraiana Rw1G002290 Rw1G026410 Rw1G026420 Rw1G026440 Rw4G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 491
AccB1I GGYRCC 2 cut(s) 191, 236
AclWI GGATC 1 cut(s) 276
AcsI RAATTY 2 cut(s) 244, 456
AfiI CCNNNNNNNGG 1 cut(s) 297
AflIII ACRYGT 2 cut(s) 691, 705
AgsI TTSAA 5 cut(s) 7, 449, 532, 581, 793
AhdI GACNNNNNGTC 1 cut(s) 421
AjnI CCWGG 3 cut(s) 187, 239, 512
AluBI AGCT 5 cut(s) 70, 106, 154, 199, 420
AluI AGCT 5 cut(s) 70, 106, 154, 199, 420
Alw26I GTCTC 1 cut(s) 696
AlwI GGATC 1 cut(s) 276
AoxI GGCC 2 cut(s) 60, 299
ApeKI GCWGC 2 cut(s) 67, 218
ApoI RAATTY 2 cut(s) 244, 456
AspS9I GGNCC 7 cut(s) 53, 61, 185, 254, 299, 406, 423
AvaII GGWCC 5 cut(s) 53, 185, 254, 406, 423
BanI GGYRCC 2 cut(s) 191, 236
BbsI GAAGAC 1 cut(s) 105
BbvI GCAGC 2 cut(s) 79, 230
BccI CCATC 4 cut(s) 290, 356, 379, 730
BceAI ACGGC 1 cut(s) 493
BciT130I CCWGG 3 cut(s) 189, 241, 514
BcoDI GTCTC 1 cut(s) 696
BfaI CTAG 4 cut(s) 71, 155, 287, 413
BfmI CTRYAG 2 cut(s) 65, 319
BfuAI ACCTGC 1 cut(s) 491
BisI GCNGC 2 cut(s) 68, 219
BlsI GCNGC 2 cut(s) 69, 220
Bme1390I CCNGG 3 cut(s) 189, 241, 514
Bme18I GGWCC 5 cut(s) 53, 185, 254, 406, 423
BmeRI GACNNNNNGTC 1 cut(s) 421
BmgT120I GGNCC 7 cut(s) 53, 61, 185, 254, 299, 406, 423
BmiI GGNNCC 4 cut(s) 193, 238, 255, 425
BmrFI CCNGG 3 cut(s) 189, 241, 514
BmsI GCATC 3 cut(s) 384, 427, 721
BpiI GAAGAC 1 cut(s) 105
BsaJI CCNNGG 2 cut(s) 475, 512
BsaWI WCCGGW 1 cut(s) 818
Bsc4I CCNNNNNNNGG 1 cut(s) 297
Bse118I RCCGGY 1 cut(s) 182
Bse3DI GCAATG 1 cut(s) 546
BseBI CCWGG 3 cut(s) 189, 241, 514
BseDI CCNNGG 2 cut(s) 475, 512
BseGI GGATG 1 cut(s) 736
BseLI CCNNNNNNNGG 1 cut(s) 297
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 3 cut(s) 240, 269, 323
BseRI GAGGAG 1 cut(s) 714
BseXI GCAGC 2 cut(s) 79, 230
BshFI GGCC 2 cut(s) 62, 301
BshNI GGYRCC 2 cut(s) 191, 236
BsiSI CCGG 2 cut(s) 183, 819
BslFI GGGAC 3 cut(s) 267, 314, 409
BslI CCNNNNNNNGG 1 cut(s) 297
BsmAI GTCTC 1 cut(s) 696
BsmFI GGGAC 3 cut(s) 267, 314, 409
BsnI GGCC 2 cut(s) 62, 301
Bsp143I GATC 1 cut(s) 268
BspANI GGCC 2 cut(s) 62, 301
BspCNI CTCAG 3 cut(s) 241, 270, 322
BspHI TCATGA 1 cut(s) 564
BspLI GGNNCC 4 cut(s) 193, 238, 255, 425
BspMAI CTGCAG 1 cut(s) 69
BspMI ACCTGC 1 cut(s) 491
BspPI GGATC 1 cut(s) 276
BspT107I GGYRCC 2 cut(s) 191, 236
BsrDI GCAATG 1 cut(s) 546
BsrFI RCCGGY 1 cut(s) 182
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 2 cut(s) 475, 512
BssMI GATC 1 cut(s) 268
Bst2UI CCWGG 3 cut(s) 189, 241, 514
Bst4CI ACNGT 1 cut(s) 53
Bst6I CTCTTC 2 cut(s) 75, 208
BstC8I GCNNGC 1 cut(s) 303
BstDEI CTNAG 5 cut(s) 17, 249, 278, 309, 647
BstDSI CCRYGG 1 cut(s) 475
BstF5I GGATG 1 cut(s) 736
BstKTI GATC 1 cut(s) 271
BstMAI GTCTC 1 cut(s) 696
BstMBI GATC 1 cut(s) 268
BstNI CCWGG 3 cut(s) 189, 241, 514
BstNSI RCATGY 2 cut(s) 695, 709
BstSCI CCNGG 3 cut(s) 187, 239, 512
BstSFI CTRYAG 2 cut(s) 65, 319
BstV1I GCAGC 2 cut(s) 79, 230
BstV2I GAAGAC 1 cut(s) 105
BstXI CCANNNNNNTGG 1 cut(s) 106
BsuRI GGCC 2 cut(s) 62, 301
BtgI CCRYGG 1 cut(s) 475
BtsCI GGATG 1 cut(s) 736
BtsI GCAGTG 2 cut(s) 238, 775
BtsIMutI CAGTG 2 cut(s) 238, 775
BveI ACCTGC 1 cut(s) 491
Cac8I GCNNGC 1 cut(s) 303
CciI TCATGA 1 cut(s) 564
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 7 cut(s) 53, 61, 185, 254, 299, 406, 423
CviAII CATG 5 cut(s) 130, 485, 565, 692, 706
DdeI CTNAG 5 cut(s) 17, 249, 278, 309, 647
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
DriI GACNNNNNGTC 1 cut(s) 421
Eam1104I CTCTTC 2 cut(s) 75, 208
Eam1105I GACNNNNNGTC 1 cut(s) 421
EarI CTCTTC 2 cut(s) 75, 208
Eco47I GGWCC 5 cut(s) 53, 185, 254, 406, 423
EcoO109I RGGNCCY 1 cut(s) 406
EcoRI GAATTC 2 cut(s) 244, 456
EcoRII CCWGG 3 cut(s) 187, 239, 512
FaeI CATG 5 cut(s) 133, 488, 568, 695, 709
FaqI GGGAC 3 cut(s) 267, 314, 409
FatI CATG 5 cut(s) 129, 484, 564, 691, 705
Fnu4HI GCNGC 2 cut(s) 68, 219
FokI GGATG 1 cut(s) 743
Fsp4HI GCNGC 2 cut(s) 68, 219
FspBI CTAG 4 cut(s) 71, 155, 287, 413
GluI GCNGC 2 cut(s) 68, 219
HaeIII GGCC 2 cut(s) 62, 301
HapII CCGG 2 cut(s) 183, 819
Hin1II CATG 5 cut(s) 133, 488, 568, 695, 709
HinfI GANTC 3 cut(s) 471, 574, 639
HpaII CCGG 2 cut(s) 183, 819
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 4 cut(s) 211, 250, 279, 312
Hpy188III TCNNGA 2 cut(s) 565, 758
Hpy8I GTNNAC 1 cut(s) 475
HpyAV CCTTC 1 cut(s) 135
HpyCH4III ACNGT 1 cut(s) 53
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 7 cut(s) 67, 139, 218, 397, 440, 500, 713
HpyF3I CTNAG 5 cut(s) 17, 249, 278, 309, 647
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 5 cut(s) 133, 488, 568, 695, 709
Kzo9I GATC 1 cut(s) 268
Lsp1109I GCAGC 2 cut(s) 79, 230
LweI GCATC 3 cut(s) 384, 427, 721
MaeI CTAG 4 cut(s) 71, 155, 287, 413
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 4 cut(s) 349, 640, 767, 799
MalI GATC 1 cut(s) 270
MboI GATC 1 cut(s) 268
MluCI AATT 3 cut(s) 244, 456, 795
MlyI GAGTC 2 cut(s) 480, 648
MmeI TCCRAC 3 cut(s) 246, 363, 676
MnlI CCTC 6 cut(s) 244, 273, 306, 419, 446, 692
MseI TTAA 7 cut(s) 27, 465, 519, 546, 678, 741, 749
MslI CAYNNNNRTG 2 cut(s) 134, 539
MspA1I CMGCKG 1 cut(s) 420
MspI CCGG 2 cut(s) 183, 819
MspR9I CCNGG 3 cut(s) 189, 241, 514
MvaI CCWGG 3 cut(s) 189, 241, 514
NdeII GATC 1 cut(s) 268
NlaIII CATG 5 cut(s) 133, 488, 568, 695, 709
NlaIV GGNNCC 4 cut(s) 193, 238, 255, 425
NmuCI GTSAC 3 cut(s) 349, 640, 767
NspI RCATGY 2 cut(s) 695, 709
PagI TCATGA 1 cut(s) 564
PciI ACATGT 2 cut(s) 691, 705
PfeI GAWTC 1 cut(s) 574
PflFI GACNNNGTC 1 cut(s) 404
PkrI GCNGC 2 cut(s) 69, 220
PleI GAGTC 2 cut(s) 479, 647
PpsI GAGTC 2 cut(s) 479, 647
PpuMI RGGWCCY 1 cut(s) 406
PscI ACATGT 2 cut(s) 691, 705
Psp5II RGGWCCY 1 cut(s) 406
Psp6I CCWGG 3 cut(s) 187, 239, 512
PspGI CCWGG 3 cut(s) 187, 239, 512
PspN4I GGNNCC 4 cut(s) 193, 238, 255, 425
PspPI GGNCC 7 cut(s) 53, 61, 185, 254, 299, 406, 423
PspPPI RGGWCCY 1 cut(s) 406
PstI CTGCAG 1 cut(s) 69
PsyI GACNNNGTC 1 cut(s) 404
PvuII CAGCTG 1 cut(s) 420
RseI CAYNNNNRTG 2 cut(s) 134, 539
SaqAI TTAA 7 cut(s) 27, 465, 519, 546, 678, 741, 749
SatI GCNGC 2 cut(s) 68, 219
Sau3AI GATC 1 cut(s) 268
Sau96I GGNCC 7 cut(s) 53, 61, 185, 254, 299, 406, 423
SchI GAGTC 2 cut(s) 480, 648
ScrFI CCNGG 3 cut(s) 189, 241, 514
SfaNI GCATC 3 cut(s) 384, 427, 721
SfcI CTRYAG 2 cut(s) 65, 319
SinI GGWCC 5 cut(s) 53, 185, 254, 406, 423
SmiMI CAYNNNNRTG 2 cut(s) 134, 539
Sse9I AATT 3 cut(s) 244, 456, 795
SspMI CTAG 4 cut(s) 71, 155, 287, 413
StyD4I CCNGG 3 cut(s) 187, 239, 512
TaaI ACNGT 1 cut(s) 53
TaiI ACGT 1 cut(s) 348
TaqI TCGA 2 cut(s) 150, 165
TasI AATT 3 cut(s) 244, 456, 795
TfiI GAWTC 1 cut(s) 574
Tru1I TTAA 7 cut(s) 27, 465, 519, 546, 678, 741, 749
Tru9I TTAA 7 cut(s) 27, 465, 519, 546, 678, 741, 749
TscAI CASTG 2 cut(s) 238, 782
TseFI GTSAC 3 cut(s) 349, 640, 767
TseI GCWGC 2 cut(s) 67, 218
Tsp45I GTSAC 3 cut(s) 349, 640, 767
TspDTI ATGAA 3 cut(s) 195, 372, 581
TspRI CASTG 2 cut(s) 238, 782
Tth111I GACNNNGTC 1 cut(s) 404
VpaK11BI GGWCC 5 cut(s) 53, 185, 254, 406, 423
XapI RAATTY 2 cut(s) 244, 456
XceI RCATGY 2 cut(s) 695, 709
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XspI CTAG 4 cut(s) 71, 155, 287, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.