Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 950 |
| Acc36I | ACCTGC | 1 cut(s) | 950 |
| AccB1I | GGYRCC | 4 cut(s) | 179, 196, 374, 385 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 872 |
| AciI | CCGC | 2 cut(s) | 137, 143 |
| AclWI | GGATC | 1 cut(s) | 219 |
| AcsI | RAATTY | 2 cut(s) | 573, 1004 |
| AcuI | CTGAAG | 1 cut(s) | 22 |
| AcyI | GRCGYC | 3 cut(s) | 197, 375, 386 |
| AdeI | CACNNNGTG | 1 cut(s) | 244 |
| AfaI | GTAC | 2 cut(s) | 338, 587 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 872 |
| AflII | CTTAAG | 1 cut(s) | 605 |
| AflIII | ACRYGT | 2 cut(s) | 742, 756 |
| AgsI | TTSAA | 3 cut(s) | 433, 532, 835 |
| AjnI | CCWGG | 3 cut(s) | 499, 512, 913 |
| AleI | CACNNNNGTG | 1 cut(s) | 734 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 407, 439 |
| AluBI | AGCT | 9 cut(s) | 36, 42, 55, 103, 131, 170, 175, 420, 838 |
| AluI | AGCT | 9 cut(s) | 36, 42, 55, 103, 131, 170, 175, 420, 838 |
| Alw21I | GWGCWC | 4 cut(s) | 249, 603, 828, 941 |
| Alw26I | GTCTC | 1 cut(s) | 747 |
| AlwI | GGATC | 1 cut(s) | 219 |
| Ama87I | CYCGRG | 1 cut(s) | 191 |
| AoxI | GGCC | 2 cut(s) | 293, 631 |
| ApeKI | GCWGC | 6 cut(s) | 52, 73, 167, 536, 929, 1025 |
| ApoI | RAATTY | 2 cut(s) | 573, 1004 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 897 |
| AspLEI | GCGC | 3 cut(s) | 199, 377, 388 |
| AspS9I | GGNCC | 4 cut(s) | 293, 472, 508, 918 |
| AsuC2I | CCSGG | 1 cut(s) | 978 |
| AsuHPI | GGTGA | 1 cut(s) | 70 |
| AvaI | CYCGRG | 1 cut(s) | 191 |
| AvaII | GGWCC | 3 cut(s) | 472, 508, 918 |
| BanI | GGYRCC | 4 cut(s) | 179, 196, 374, 385 |
| BbsI | GAAGAC | 1 cut(s) | 400 |
| Bbv12I | GWGCWC | 4 cut(s) | 249, 603, 828, 941 |
| BbvI | GCAGC | 6 cut(s) | 60, 64, 179, 548, 916, 1012 |
| BccI | CCATC | 2 cut(s) | 284, 376 |
| BciT130I | CCWGG | 3 cut(s) | 501, 514, 915 |
| BciVI | GTATCC | 2 cut(s) | 507, 795 |
| BclI | TGATCA | 1 cut(s) | 280 |
| BcnI | CCSGG | 1 cut(s) | 978 |
| BcoDI | GTCTC | 1 cut(s) | 747 |
| BfaI | CTAG | 1 cut(s) | 176 |
| BfoI | RGCGCY | 3 cut(s) | 200, 378, 389 |
| BfrI | CTTAAG | 1 cut(s) | 605 |
| BfuAI | ACCTGC | 1 cut(s) | 950 |
| BfuI | GTATCC | 2 cut(s) | 507, 795 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 383 |
| BisI | GCNGC | 6 cut(s) | 53, 74, 168, 537, 930, 1026 |
| BlpI | GCTNAGC | 1 cut(s) | 171 |
| BlsI | GCNGC | 6 cut(s) | 54, 75, 169, 538, 931, 1027 |
| BmcAI | AGTACT | 1 cut(s) | 338 |
| Bme1390I | CCNGG | 4 cut(s) | 501, 514, 915, 978 |
| Bme18I | GGWCC | 3 cut(s) | 472, 508, 918 |
| BmeT110I | CYCGRG | 1 cut(s) | 191 |
| BmgT120I | GGNCC | 4 cut(s) | 293, 472, 508, 918 |
| BmiI | GGNNCC | 8 cut(s) | 181, 198, 376, 387, 425, 473, 510, 919 |
| BmrFI | CCNGG | 4 cut(s) | 501, 514, 915, 978 |
| BmsI | GCATC | 2 cut(s) | 693, 772 |
| BpiI | GAAGAC | 1 cut(s) | 400 |
| BpmI | CTGGAG | 1 cut(s) | 753 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 171 |
| BpuEI | CTTGAG | 1 cut(s) | 381 |
| BpuMI | CCSGG | 1 cut(s) | 978 |
| BsaBI | GATNNNNATC | 1 cut(s) | 279 |
| BsaHI | GRCGYC | 3 cut(s) | 197, 375, 386 |
| BsaJI | CCNNGG | 6 cut(s) | 192, 475, 512, 634, 914, 1014 |
| BsaWI | WCCGGW | 1 cut(s) | 426 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 872 |
| Bse1I | ACTGG | 3 cut(s) | 736, 818, 905 |
| Bse3DI | GCAATG | 1 cut(s) | 546 |
| Bse8I | GATNNNNATC | 1 cut(s) | 279 |
| BseBI | CCWGG | 3 cut(s) | 501, 514, 915 |
| BseDI | CCNNGG | 6 cut(s) | 192, 475, 512, 634, 914, 1014 |
| BseGI | GGATG | 3 cut(s) | 155, 787, 958 |
| BseJI | GATNNNNATC | 1 cut(s) | 279 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 872 |
| BseMI | GCAATG | 1 cut(s) | 546 |
| BseMII | CTCAG | 2 cut(s) | 185, 235 |
| BseNI | ACTGG | 3 cut(s) | 736, 818, 905 |
| BseRI | GAGGAG | 1 cut(s) | 765 |
| BseXI | GCAGC | 6 cut(s) | 60, 64, 179, 548, 916, 1012 |
| BshFI | GGCC | 2 cut(s) | 295, 633 |
| BshNI | GGYRCC | 4 cut(s) | 179, 196, 374, 385 |
| BsiHKAI | GWGCWC | 4 cut(s) | 249, 603, 828, 941 |
| BsiHKCI | CYCGRG | 1 cut(s) | 191 |
| BsiSI | CCGG | 2 cut(s) | 427, 977 |
| BslFI | GGGAC | 1 cut(s) | 931 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 872 |
| BsmAI | GTCTC | 1 cut(s) | 747 |
| BsmFI | GGGAC | 1 cut(s) | 931 |
| BsnI | GGCC | 2 cut(s) | 295, 633 |
| BsoBI | CYCGRG | 1 cut(s) | 191 |
| Bsp1286I | GDGCHC | 4 cut(s) | 249, 603, 828, 941 |
| Bsp1407I | TGTACA | 1 cut(s) | 585 |
| Bsp143I | GATC | 3 cut(s) | 211, 280, 580 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 171 |
| Bsp19I | CCATGG | 1 cut(s) | 475 |
| BspACI | CCGC | 2 cut(s) | 137, 143 |
| BspANI | GGCC | 2 cut(s) | 295, 633 |
| BspCNI | CTCAG | 2 cut(s) | 184, 234 |
| BspHI | TCATGA | 1 cut(s) | 808 |
| BspLI | GGNNCC | 8 cut(s) | 181, 198, 376, 387, 425, 473, 510, 919 |
| BspMI | ACCTGC | 1 cut(s) | 950 |
| BspPI | GGATC | 1 cut(s) | 219 |
| BspT107I | GGYRCC | 4 cut(s) | 179, 196, 374, 385 |
| BspTI | CTTAAG | 1 cut(s) | 605 |
| BsrDI | GCAATG | 1 cut(s) | 546 |
| BsrGI | TGTACA | 1 cut(s) | 585 |
| BsrI | ACTGG | 3 cut(s) | 736, 818, 905 |
| BssECI | CCNNGG | 6 cut(s) | 192, 475, 512, 634, 914, 1014 |
| BssMI | GATC | 3 cut(s) | 211, 280, 580 |
| BssNI | GRCGYC | 3 cut(s) | 197, 375, 386 |
| BssT1I | CCWWGG | 3 cut(s) | 475, 634, 1014 |
| Bst2UI | CCWGG | 3 cut(s) | 501, 514, 915 |
| Bst4CI | ACNGT | 1 cut(s) | 259 |
| Bst6I | CTCTTC | 1 cut(s) | 341 |
| BstACI | GRCGYC | 3 cut(s) | 197, 375, 386 |
| BstAFI | CTTAAG | 1 cut(s) | 605 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1025 |
| BstAUI | TGTACA | 1 cut(s) | 585 |
| BstDEI | CTNAG | 3 cut(s) | 171, 221, 647 |
| BstDSI | CCRYGG | 1 cut(s) | 475 |
| BstF5I | GGATG | 3 cut(s) | 155, 787, 958 |
| BstH2I | RGCGCY | 3 cut(s) | 200, 378, 389 |
| BstHHI | GCGC | 3 cut(s) | 199, 377, 388 |
| BstKTI | GATC | 3 cut(s) | 214, 283, 583 |
| BstMAI | GTCTC | 1 cut(s) | 747 |
| BstMBI | GATC | 3 cut(s) | 211, 280, 580 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 383, 607, 1025 |
| BstNI | CCWGG | 3 cut(s) | 501, 514, 915 |
| BstNSI | RCATGY | 4 cut(s) | 488, 719, 746, 760 |
| BstSCI | CCNGG | 4 cut(s) | 499, 512, 913, 976 |
| BstV1I | GCAGC | 6 cut(s) | 60, 64, 179, 548, 916, 1012 |
| BstV2I | GAAGAC | 1 cut(s) | 400 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 55, 208 |
| BsuI | GTATCC | 2 cut(s) | 507, 795 |
| BsuRI | GGCC | 2 cut(s) | 295, 633 |
| BtgI | CCRYGG | 1 cut(s) | 475 |
| BtsCI | GGATG | 3 cut(s) | 155, 787, 958 |
| BtsI | GCAGTG | 2 cut(s) | 826, 924 |
| BtsIMutI | CAGTG | 4 cut(s) | 255, 729, 826, 924 |
| BveI | ACCTGC | 1 cut(s) | 950 |
| CciI | TCATGA | 1 cut(s) | 808 |
| CfoI | GCGC | 3 cut(s) | 199, 377, 388 |
| Cfr13I | GGNCC | 4 cut(s) | 293, 472, 508, 918 |
| Csp6I | GTAC | 2 cut(s) | 337, 586 |
| CviQI | GTAC | 2 cut(s) | 337, 586 |
| DdeI | CTNAG | 3 cut(s) | 171, 221, 647 |
| DinI | GGCGCC | 3 cut(s) | 198, 376, 387 |
| DpnI | GATC | 3 cut(s) | 213, 282, 582 |
| DpnII | GATC | 3 cut(s) | 211, 280, 580 |
| DraIII | CACNNNGTG | 1 cut(s) | 244 |
| Eam1104I | CTCTTC | 1 cut(s) | 341 |
| EarI | CTCTTC | 1 cut(s) | 341 |
| Eco130I | CCWWGG | 3 cut(s) | 475, 634, 1014 |
| Eco47I | GGWCC | 3 cut(s) | 472, 508, 918 |
| Eco57I | CTGAAG | 1 cut(s) | 22 |
| Eco88I | CYCGRG | 1 cut(s) | 191 |
| EcoRI | GAATTC | 1 cut(s) | 1004 |
| EcoRII | CCWGG | 3 cut(s) | 499, 512, 913 |
| EcoT14I | CCWWGG | 3 cut(s) | 475, 634, 1014 |
| EgeI | GGCGCC | 3 cut(s) | 198, 376, 387 |
| EheI | GGCGCC | 3 cut(s) | 198, 376, 387 |
| ErhI | CCWWGG | 3 cut(s) | 475, 634, 1014 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 395, 427, 885, 917 |
| FaqI | GGGAC | 1 cut(s) | 931 |
| FauI | CCCGC | 2 cut(s) | 130, 136 |
| FbaI | TGATCA | 1 cut(s) | 280 |
| Fnu4HI | GCNGC | 6 cut(s) | 53, 74, 168, 537, 930, 1026 |
| FokI | GGATG | 3 cut(s) | 142, 794, 965 |
| Fsp4HI | GCNGC | 6 cut(s) | 53, 74, 168, 537, 930, 1026 |
| FspBI | CTAG | 1 cut(s) | 176 |
| GlaI | GCGC | 3 cut(s) | 198, 376, 387 |
| GluI | GCNGC | 6 cut(s) | 53, 74, 168, 537, 930, 1026 |
| GsuI | CTGGAG | 1 cut(s) | 753 |
| HaeII | RGCGCY | 3 cut(s) | 200, 378, 389 |
| HaeIII | GGCC | 2 cut(s) | 295, 633 |
| HapII | CCGG | 2 cut(s) | 427, 977 |
| HhaI | GCGC | 3 cut(s) | 199, 377, 388 |
| Hin1I | GRCGYC | 3 cut(s) | 197, 375, 386 |
| Hin6I | GCGC | 3 cut(s) | 197, 375, 386 |
| HinP1I | GCGC | 3 cut(s) | 197, 375, 386 |
| HindIII | AAGCTT | 1 cut(s) | 34 |
| HinfI | GANTC | 3 cut(s) | 394, 456, 643 |
| HpaII | CCGG | 2 cut(s) | 427, 977 |
| HphI | GGTGA | 1 cut(s) | 70 |
| Hpy166II | GTNNAC | 1 cut(s) | 9 |
| Hpy188I | TCNGA | 2 cut(s) | 306, 508 |
| Hpy188III | TCNNGA | 5 cut(s) | 398, 568, 578, 809, 841 |
| Hpy8I | GTNNAC | 1 cut(s) | 9 |
| HpyAV | CCTTC | 2 cut(s) | 443, 732 |
| HpyCH4III | ACNGT | 1 cut(s) | 259 |
| HpyCH4V | TGCA | 7 cut(s) | 52, 88, 167, 255, 546, 764, 932 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 383, 607, 1025 |
| HpyF3I | CTNAG | 3 cut(s) | 171, 221, 647 |
| Hsp92I | GRCGYC | 3 cut(s) | 197, 375, 386 |
| HspAI | GCGC | 3 cut(s) | 197, 375, 386 |
| KasI | GGCGCC | 3 cut(s) | 196, 374, 385 |
| Ksp22I | TGATCA | 1 cut(s) | 280 |
| Kzo9I | GATC | 3 cut(s) | 211, 280, 580 |
| LmnI | GCTCC | 2 cut(s) | 328, 843 |
| Lsp1109I | GCAGC | 6 cut(s) | 60, 64, 179, 548, 916, 1012 |
| LweI | GCATC | 2 cut(s) | 693, 772 |
| MaeI | CTAG | 1 cut(s) | 176 |
| MaeIII | GTNAC | 4 cut(s) | 58, 236, 640, 851 |
| MalI | GATC | 3 cut(s) | 213, 282, 582 |
| MboI | GATC | 3 cut(s) | 211, 280, 580 |
| MboII | GAAGA | 9 cut(s) | 29, 32, 35, 38, 41, 358, 368, 400, 893 |
| MfeI | CAATTG | 1 cut(s) | 250 |
| MhlI | GDGCHC | 4 cut(s) | 249, 603, 828, 941 |
| MluCI | AATT | 7 cut(s) | 250, 298, 573, 847, 882, 1004, 1066 |
| Mly113I | GGCGCC | 3 cut(s) | 197, 375, 386 |
| MlyI | GAGTC | 2 cut(s) | 388, 637 |
| MmeI | TCCRAC | 2 cut(s) | 189, 727 |
| MnlI | CCTC | 8 cut(s) | 163, 187, 300, 446, 681, 711, 743, 974 |
| MroXI | GAANNNNTTC | 1 cut(s) | 897 |
| MseI | TTAA | 6 cut(s) | 264, 465, 519, 606, 678, 1089 |
| MslI | CAYNNNNRTG | 3 cut(s) | 288, 734, 791 |
| MspA1I | CMGCKG | 2 cut(s) | 55, 420 |
| MspCI | CTTAAG | 1 cut(s) | 605 |
| MspI | CCGG | 2 cut(s) | 427, 977 |
| MspR9I | CCNGG | 4 cut(s) | 501, 514, 915, 978 |
| MunI | CAATTG | 1 cut(s) | 250 |
| MvaI | CCWGG | 3 cut(s) | 501, 514, 915 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 383, 607, 1025 |
| NarI | GGCGCC | 3 cut(s) | 197, 375, 386 |
| NciI | CCSGG | 1 cut(s) | 978 |
| NcoI | CCATGG | 1 cut(s) | 475 |
| NdeII | GATC | 3 cut(s) | 211, 280, 580 |
| NlaIV | GGNNCC | 8 cut(s) | 181, 198, 376, 387, 425, 473, 510, 919 |
| NmuCI | GTSAC | 3 cut(s) | 58, 236, 640 |
| NspI | RCATGY | 4 cut(s) | 488, 719, 746, 760 |
| OliI | CACNNNNGTG | 1 cut(s) | 734 |
| PagI | TCATGA | 1 cut(s) | 808 |
| PaqCI | CACCTGC | 1 cut(s) | 950 |
| PciI | ACATGT | 2 cut(s) | 742, 756 |
| PdmI | GAANNNNTTC | 1 cut(s) | 897 |
| PfeI | GAWTC | 1 cut(s) | 456 |
| PflFI | GACNNNGTC | 1 cut(s) | 404 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 872 |
| PkrI | GCNGC | 6 cut(s) | 54, 75, 169, 538, 931, 1027 |
| PleI | GAGTC | 2 cut(s) | 388, 637 |
| PluTI | GGCGCC | 3 cut(s) | 200, 378, 389 |
| PpsI | GAGTC | 2 cut(s) | 388, 637 |
| PscI | ACATGT | 2 cut(s) | 742, 756 |
| Psp6I | CCWGG | 3 cut(s) | 499, 512, 913 |
| PspGI | CCWGG | 3 cut(s) | 499, 512, 913 |
| PspN4I | GGNNCC | 8 cut(s) | 181, 198, 376, 387, 425, 473, 510, 919 |
| PspPI | GGNCC | 4 cut(s) | 293, 472, 508, 918 |
| PsyI | GACNNNGTC | 1 cut(s) | 404 |
| PvuII | CAGCTG | 2 cut(s) | 55, 420 |
| RsaI | GTAC | 2 cut(s) | 338, 587 |
| RsaNI | GTAC | 2 cut(s) | 337, 586 |
| RseI | CAYNNNNRTG | 3 cut(s) | 288, 734, 791 |
| SaqAI | TTAA | 6 cut(s) | 264, 465, 519, 606, 678, 1089 |
| SatI | GCNGC | 6 cut(s) | 53, 74, 168, 537, 930, 1026 |
| Sau3AI | GATC | 3 cut(s) | 211, 280, 580 |
| Sau96I | GGNCC | 4 cut(s) | 293, 472, 508, 918 |
| ScaI | AGTACT | 1 cut(s) | 338 |
| SchI | GAGTC | 2 cut(s) | 388, 637 |
| ScrFI | CCNGG | 4 cut(s) | 501, 514, 915, 978 |
| SduI | GDGCHC | 4 cut(s) | 249, 603, 828, 941 |
| SfaNI | GCATC | 2 cut(s) | 693, 772 |
| SfoI | GGCGCC | 3 cut(s) | 198, 376, 387 |
| SinI | GGWCC | 3 cut(s) | 472, 508, 918 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 288, 734, 791 |
| SmlI | CTYRAG | 2 cut(s) | 396, 605 |
| SmoI | CTYRAG | 2 cut(s) | 396, 605 |
| Sse9I | AATT | 7 cut(s) | 250, 298, 573, 847, 882, 1004, 1066 |
| SsiI | CCGC | 2 cut(s) | 137, 143 |
| SspDI | GGCGCC | 3 cut(s) | 196, 374, 385 |
| SspI | AATATT | 1 cut(s) | 1055 |
| SspMI | CTAG | 1 cut(s) | 176 |
| StyD4I | CCNGG | 4 cut(s) | 499, 512, 913, 976 |
| StyI | CCWWGG | 3 cut(s) | 475, 634, 1014 |
| TaaI | ACNGT | 1 cut(s) | 259 |
| TaqI | TCGA | 2 cut(s) | 114, 214 |
| TasI | AATT | 7 cut(s) | 250, 298, 573, 847, 882, 1004, 1066 |
| TatI | WGTACW | 2 cut(s) | 336, 585 |
| TfiI | GAWTC | 1 cut(s) | 456 |
| Tru1I | TTAA | 6 cut(s) | 264, 465, 519, 606, 678, 1089 |
| Tru9I | TTAA | 6 cut(s) | 264, 465, 519, 606, 678, 1089 |
| TscAI | CASTG | 4 cut(s) | 262, 736, 833, 931 |
| TseFI | GTSAC | 3 cut(s) | 58, 236, 640 |
| TseI | GCWGC | 6 cut(s) | 52, 73, 167, 536, 929, 1025 |
| Tsp45I | GTSAC | 3 cut(s) | 58, 236, 640 |
| TspDTI | ATGAA | 5 cut(s) | 369, 797, 809, 997, 1065 |
| TspRI | CASTG | 4 cut(s) | 262, 736, 833, 931 |
| Tth111I | GACNNNGTC | 1 cut(s) | 404 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 872 |
| Vha464I | CTTAAG | 1 cut(s) | 605 |
| VpaK11BI | GGWCC | 3 cut(s) | 472, 508, 918 |
| XapI | RAATTY | 2 cut(s) | 573, 1004 |
| XceI | RCATGY | 4 cut(s) | 488, 719, 746, 760 |
| XmnI | GAANNNNTTC | 1 cut(s) | 897 |
| XspI | CTAG | 1 cut(s) | 176 |
| ZrmI | AGTACT | 1 cut(s) | 338 |