Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 704 |
| Acc36I | ACCTGC | 1 cut(s) | 158 |
| AccB1I | GGYRCC | 1 cut(s) | 215 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 866 |
| AccII | CGCG | 1 cut(s) | 329 |
| AciI | CCGC | 5 cut(s) | 179, 327, 329, 565, 695 |
| AclWI | GGATC | 1 cut(s) | 255 |
| AcoI | YGGCCR | 1 cut(s) | 304 |
| AcsI | RAATTY | 1 cut(s) | 450 |
| AcuI | CTGAAG | 1 cut(s) | 372 |
| AdeI | CACNNNGTG | 1 cut(s) | 346 |
| AfaI | GTAC | 1 cut(s) | 282 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 397, 725, 866 |
| AflIII | ACRYGT | 2 cut(s) | 736, 750 |
| AgsI | TTSAA | 4 cut(s) | 443, 526, 541, 838 |
| AjnI | CCWGG | 1 cut(s) | 724 |
| AluBI | AGCT | 6 cut(s) | 32, 72, 91, 139, 607, 635 |
| AluI | AGCT | 6 cut(s) | 32, 72, 91, 139, 607, 635 |
| Alw21I | GWGCWC | 2 cut(s) | 141, 597 |
| Alw26I | GTCTC | 1 cut(s) | 741 |
| AlwI | GGATC | 1 cut(s) | 255 |
| Ama87I | CYCGRG | 1 cut(s) | 505 |
| AoxI | GGCC | 2 cut(s) | 3, 304 |
| ApeKI | GCWGC | 4 cut(s) | 88, 109, 203, 233 |
| ApoI | RAATTY | 1 cut(s) | 450 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 72 |
| AspLEI | GCGC | 1 cut(s) | 109 |
| AspS9I | GGNCC | 1 cut(s) | 502 |
| AsuHPI | GGTGA | 2 cut(s) | 707, 951 |
| AvaI | CYCGRG | 1 cut(s) | 505 |
| AvaII | GGWCC | 1 cut(s) | 502 |
| AxyI | CCTNAGG | 2 cut(s) | 228, 257 |
| BalI | TGGCCA | 1 cut(s) | 306 |
| BanI | GGYRCC | 1 cut(s) | 215 |
| BanII | GRGCYC | 2 cut(s) | 141, 186 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 830, 862 |
| BbsI | GAAGAC | 1 cut(s) | 394 |
| Bbv12I | GWGCWC | 2 cut(s) | 141, 597 |
| BbvI | GCAGC | 4 cut(s) | 96, 100, 190, 245 |
| BccI | CCATC | 3 cut(s) | 296, 350, 860 |
| BciT130I | CCWGG | 1 cut(s) | 726 |
| BcoDI | GTCTC | 1 cut(s) | 741 |
| BfmI | CTRYAG | 3 cut(s) | 33, 316, 580 |
| BfuAI | ACCTGC | 1 cut(s) | 158 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 913 |
| BisI | GCNGC | 5 cut(s) | 89, 110, 204, 234, 695 |
| BlsI | GCNGC | 5 cut(s) | 90, 111, 205, 235, 696 |
| Bme1390I | CCNGG | 1 cut(s) | 726 |
| Bme18I | GGWCC | 1 cut(s) | 502 |
| BmeT110I | CYCGRG | 1 cut(s) | 505 |
| BmgT120I | GGNCC | 1 cut(s) | 502 |
| BmiI | GGNNCC | 4 cut(s) | 217, 263, 467, 504 |
| BmrFI | CCNGG | 1 cut(s) | 726 |
| BmsI | GCATC | 1 cut(s) | 766 |
| BpiI | GAAGAC | 1 cut(s) | 394 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 624, 656 |
| BpmI | CTGGAG | 1 cut(s) | 843 |
| BsaJI | CCNNGG | 5 cut(s) | 327, 469, 506, 598, 908 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 308, 338 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 397, 725, 866 |
| Bse1I | ACTGG | 4 cut(s) | 18, 899, 925, 974 |
| Bse21I | CCTNAGG | 2 cut(s) | 228, 257 |
| Bse3DI | GCAATG | 1 cut(s) | 540 |
| BseBI | CCWGG | 1 cut(s) | 726 |
| BseDI | CCNNGG | 5 cut(s) | 327, 469, 506, 598, 908 |
| BseGI | GGATG | 2 cut(s) | 820, 978 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 397, 725, 866 |
| BseMI | GCAATG | 1 cut(s) | 540 |
| BseMII | CTCAG | 3 cut(s) | 219, 655, 941 |
| BseNI | ACTGG | 4 cut(s) | 18, 899, 925, 974 |
| BseRI | GAGGAG | 2 cut(s) | 254, 759 |
| BseXI | GCAGC | 4 cut(s) | 96, 100, 190, 245 |
| BseYI | CCCAGC | 1 cut(s) | 367 |
| BsgI | GTGCAG | 2 cut(s) | 107, 174 |
| Bsh1236I | CGCG | 1 cut(s) | 329 |
| BshFI | GGCC | 2 cut(s) | 5, 306 |
| BshNI | GGYRCC | 1 cut(s) | 215 |
| BsiHKAI | GWGCWC | 2 cut(s) | 141, 597 |
| BsiHKCI | CYCGRG | 1 cut(s) | 505 |
| BsiSI | CCGG | 1 cut(s) | 934 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 397, 725, 866 |
| BsmAI | GTCTC | 1 cut(s) | 741 |
| BsnI | GGCC | 2 cut(s) | 5, 306 |
| BsoBI | CYCGRG | 1 cut(s) | 505 |
| Bsp1286I | GDGCHC | 3 cut(s) | 141, 186, 597 |
| Bsp143I | GATC | 4 cut(s) | 247, 574, 688, 956 |
| Bsp19I | CCATGG | 1 cut(s) | 469 |
| BspACI | CCGC | 5 cut(s) | 179, 327, 329, 565, 695 |
| BspANI | GGCC | 2 cut(s) | 5, 306 |
| BspCNI | CTCAG | 3 cut(s) | 220, 654, 942 |
| BspFNI | CGCG | 1 cut(s) | 329 |
| BspLI | GGNNCC | 4 cut(s) | 217, 263, 467, 504 |
| BspMI | ACCTGC | 1 cut(s) | 158 |
| BspPI | GGATC | 1 cut(s) | 255 |
| BspT107I | GGYRCC | 1 cut(s) | 215 |
| BsrDI | GCAATG | 1 cut(s) | 540 |
| BsrI | ACTGG | 4 cut(s) | 18, 899, 925, 974 |
| BssECI | CCNNGG | 5 cut(s) | 327, 469, 506, 598, 908 |
| BssMI | GATC | 4 cut(s) | 247, 574, 688, 956 |
| BssT1I | CCWWGG | 3 cut(s) | 469, 598, 908 |
| Bst2UI | CCWGG | 1 cut(s) | 726 |
| Bst4CI | ACNGT | 2 cut(s) | 413, 851 |
| Bst6I | CTCTTC | 1 cut(s) | 13 |
| BstC8I | GCNNGC | 1 cut(s) | 476 |
| BstDEI | CTNAG | 4 cut(s) | 228, 257, 641, 950 |
| BstDSI | CCRYGG | 2 cut(s) | 327, 469 |
| BstF5I | GGATG | 2 cut(s) | 820, 978 |
| BstFNI | CGCG | 1 cut(s) | 329 |
| BstHHI | GCGC | 1 cut(s) | 109 |
| BstKTI | GATC | 4 cut(s) | 250, 577, 691, 959 |
| BstMAI | GTCTC | 1 cut(s) | 741 |
| BstMBI | GATC | 4 cut(s) | 247, 574, 688, 956 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 319, 913 |
| BstNI | CCWGG | 1 cut(s) | 726 |
| BstNSI | RCATGY | 4 cut(s) | 478, 701, 740, 754 |
| BstSCI | CCNGG | 1 cut(s) | 724 |
| BstSFI | CTRYAG | 3 cut(s) | 33, 316, 580 |
| BstUI | CGCG | 1 cut(s) | 329 |
| BstV1I | GCAGC | 4 cut(s) | 96, 100, 190, 245 |
| BstV2I | GAAGAC | 1 cut(s) | 394 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 969 |
| Bsu36I | CCTNAGG | 2 cut(s) | 228, 257 |
| BsuRI | GGCC | 2 cut(s) | 5, 306 |
| BtgI | CCRYGG | 2 cut(s) | 327, 469 |
| BtsCI | GGATG | 2 cut(s) | 820, 978 |
| BtsIMutI | CAGTG | 2 cut(s) | 11, 856 |
| BveI | ACCTGC | 1 cut(s) | 158 |
| Cac8I | GCNNGC | 1 cut(s) | 476 |
| CfoI | GCGC | 1 cut(s) | 109 |
| Cfr13I | GGNCC | 1 cut(s) | 502 |
| Cfr42I | CCGCGG | 1 cut(s) | 330 |
| CseI | GACGC | 2 cut(s) | 440, 896 |
| Csp6I | GTAC | 1 cut(s) | 281 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 538, 573 |
| CviAII | CATG | 9 cut(s) | 115, 121, 379, 470, 475, 698, 737, 751, 761 |
| CviQI | GTAC | 1 cut(s) | 281 |
| DdeI | CTNAG | 4 cut(s) | 228, 257, 641, 950 |
| DpnI | GATC | 4 cut(s) | 249, 576, 690, 958 |
| DpnII | GATC | 4 cut(s) | 247, 574, 688, 956 |
| DraIII | CACNNNGTG | 1 cut(s) | 346 |
| EaeI | YGGCCR | 1 cut(s) | 304 |
| Eam1104I | CTCTTC | 1 cut(s) | 13 |
| EarI | CTCTTC | 1 cut(s) | 13 |
| Ecl136II | GAGCTC | 1 cut(s) | 139 |
| Eco130I | CCWWGG | 3 cut(s) | 469, 598, 908 |
| Eco24I | GRGCYC | 2 cut(s) | 141, 186 |
| Eco32I | GATATC | 1 cut(s) | 154 |
| Eco47I | GGWCC | 1 cut(s) | 502 |
| Eco53kI | GAGCTC | 1 cut(s) | 139 |
| Eco57I | CTGAAG | 1 cut(s) | 372 |
| Eco81I | CCTNAGG | 2 cut(s) | 228, 257 |
| Eco88I | CYCGRG | 1 cut(s) | 505 |
| EcoICRI | GAGCTC | 1 cut(s) | 139 |
| EcoRI | GAATTC | 1 cut(s) | 450 |
| EcoRII | CCWGG | 1 cut(s) | 724 |
| EcoRV | GATATC | 1 cut(s) | 154 |
| EcoT14I | CCWWGG | 3 cut(s) | 469, 598, 908 |
| EcoT38I | GRGCYC | 2 cut(s) | 141, 186 |
| ErhI | CCWWGG | 3 cut(s) | 469, 598, 908 |
| FaeI | CATG | 9 cut(s) | 118, 124, 382, 473, 478, 701, 740, 754, 764 |
| FatI | CATG | 9 cut(s) | 114, 120, 378, 469, 474, 697, 736, 750, 760 |
| FauI | CCCGC | 1 cut(s) | 172 |
| Fnu4HI | GCNGC | 5 cut(s) | 89, 110, 204, 234, 695 |
| FokI | GGATG | 1 cut(s) | 827 |
| FriOI | GRGCYC | 2 cut(s) | 141, 186 |
| Fsp4HI | GCNGC | 5 cut(s) | 89, 110, 204, 234, 695 |
| GlaI | GCGC | 1 cut(s) | 108 |
| GluI | GCNGC | 5 cut(s) | 89, 110, 204, 234, 695 |
| GsaI | CCCAGC | 1 cut(s) | 371 |
| GsuI | CTGGAG | 1 cut(s) | 843 |
| HaeIII | GGCC | 2 cut(s) | 5, 306 |
| HapII | CCGG | 1 cut(s) | 934 |
| HgaI | GACGC | 2 cut(s) | 440, 896 |
| HhaI | GCGC | 1 cut(s) | 109 |
| Hin1II | CATG | 9 cut(s) | 118, 124, 382, 473, 478, 701, 740, 754, 764 |
| Hin6I | GCGC | 1 cut(s) | 107 |
| HinP1I | GCGC | 1 cut(s) | 107 |
| HindIII | AAGCTT | 1 cut(s) | 70 |
| HinfI | GANTC | 4 cut(s) | 223, 252, 568, 678 |
| HpaII | CCGG | 1 cut(s) | 934 |
| HphI | GGTGA | 2 cut(s) | 707, 951 |
| Hpy188I | TCNGA | 2 cut(s) | 143, 502 |
| Hpy188III | TCNNGA | 6 cut(s) | 41, 227, 572, 657, 803, 860 |
| HpyAV | CCTTC | 2 cut(s) | 437, 729 |
| HpyCH4III | ACNGT | 2 cut(s) | 413, 851 |
| HpyCH4V | TGCA | 4 cut(s) | 88, 124, 191, 825 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 319, 913 |
| HpyF3I | CTNAG | 4 cut(s) | 228, 257, 641, 950 |
| Hsp92II | CATG | 9 cut(s) | 118, 124, 382, 473, 478, 701, 740, 754, 764 |
| HspAI | GCGC | 1 cut(s) | 107 |
| KspI | CCGCGG | 1 cut(s) | 330 |
| Kzo9I | GATC | 4 cut(s) | 247, 574, 688, 956 |
| LmnI | GCTCC | 3 cut(s) | 267, 604, 632 |
| Lsp1109I | GCAGC | 4 cut(s) | 96, 100, 190, 245 |
| LweI | GCATC | 1 cut(s) | 766 |
| MaeIII | GTNAC | 3 cut(s) | 346, 417, 845 |
| MalI | GATC | 4 cut(s) | 249, 576, 690, 958 |
| MboI | GATC | 4 cut(s) | 247, 574, 688, 956 |
| MboII | GAAGA | 8 cut(s) | 74, 77, 365, 394, 850, 887, 913, 966 |
| MhlI | GDGCHC | 3 cut(s) | 141, 186, 597 |
| MlsI | TGGCCA | 1 cut(s) | 306 |
| MluCI | AATT | 3 cut(s) | 450, 682, 841 |
| MluNI | TGGCCA | 1 cut(s) | 306 |
| MmeI | TCCRAC | 1 cut(s) | 721 |
| MnlI | CCTC | 9 cut(s) | 16, 223, 275, 456, 501, 675, 737, 811, 906 |
| Mox20I | TGGCCA | 1 cut(s) | 306 |
| MroXI | GAANNNNTTC | 1 cut(s) | 72 |
| MscI | TGGCCA | 1 cut(s) | 306 |
| MseI | TTAA | 3 cut(s) | 513, 672, 788 |
| MslI | CAYNNNNRTG | 2 cut(s) | 119, 533 |
| Msp20I | TGGCCA | 1 cut(s) | 306 |
| MspA1I | CMGCKG | 2 cut(s) | 91, 329 |
| MspI | CCGG | 1 cut(s) | 934 |
| MspR9I | CCNGG | 1 cut(s) | 726 |
| MvaI | CCWGG | 1 cut(s) | 726 |
| MvnI | CGCG | 1 cut(s) | 329 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 319, 913 |
| NcoI | CCATGG | 1 cut(s) | 469 |
| NdeII | GATC | 4 cut(s) | 247, 574, 688, 956 |
| NlaIII | CATG | 9 cut(s) | 118, 124, 382, 473, 478, 701, 740, 754, 764 |
| NlaIV | GGNNCC | 4 cut(s) | 217, 263, 467, 504 |
| NmuCI | GTSAC | 2 cut(s) | 346, 417 |
| NspI | RCATGY | 4 cut(s) | 478, 701, 740, 754 |
| PaeI | GCATGC | 1 cut(s) | 478 |
| PciI | ACATGT | 2 cut(s) | 736, 750 |
| PdmI | GAANNNNTTC | 1 cut(s) | 72 |
| PfeI | GAWTC | 4 cut(s) | 223, 252, 568, 678 |
| PflFI | GACNNNGTC | 1 cut(s) | 398 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 866 |
| PkrI | GCNGC | 5 cut(s) | 90, 111, 205, 235, 696 |
| PscI | ACATGT | 2 cut(s) | 736, 750 |
| PsiI | TTATAA | 1 cut(s) | 704 |
| Psp124BI | GAGCTC | 1 cut(s) | 141 |
| Psp6I | CCWGG | 1 cut(s) | 724 |
| PspFI | CCCAGC | 1 cut(s) | 367 |
| PspGI | CCWGG | 1 cut(s) | 724 |
| PspN4I | GGNNCC | 4 cut(s) | 217, 263, 467, 504 |
| PspPI | GGNCC | 1 cut(s) | 502 |
| PsyI | GACNNNGTC | 1 cut(s) | 398 |
| PvuII | CAGCTG | 1 cut(s) | 91 |
| RsaI | GTAC | 1 cut(s) | 282 |
| RsaNI | GTAC | 1 cut(s) | 281 |
| RseI | CAYNNNNRTG | 2 cut(s) | 119, 533 |
| SacI | GAGCTC | 1 cut(s) | 141 |
| SacII | CCGCGG | 1 cut(s) | 330 |
| SaqAI | TTAA | 3 cut(s) | 513, 672, 788 |
| SatI | GCNGC | 5 cut(s) | 89, 110, 204, 234, 695 |
| Sau3AI | GATC | 4 cut(s) | 247, 574, 688, 956 |
| Sau96I | GGNCC | 1 cut(s) | 502 |
| ScrFI | CCNGG | 1 cut(s) | 726 |
| SduI | GDGCHC | 3 cut(s) | 141, 186, 597 |
| SfaNI | GCATC | 1 cut(s) | 766 |
| SfcI | CTRYAG | 3 cut(s) | 33, 316, 580 |
| Sfr303I | CCGCGG | 1 cut(s) | 330 |
| SgrBI | CCGCGG | 1 cut(s) | 330 |
| SinI | GGWCC | 1 cut(s) | 502 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 119, 533 |
| SphI | GCATGC | 1 cut(s) | 478 |
| Sse9I | AATT | 3 cut(s) | 450, 682, 841 |
| SsiI | CCGC | 5 cut(s) | 179, 327, 329, 565, 695 |
| SstI | GAGCTC | 1 cut(s) | 141 |
| StyD4I | CCNGG | 1 cut(s) | 724 |
| StyI | CCWWGG | 3 cut(s) | 469, 598, 908 |
| TaaI | ACNGT | 2 cut(s) | 413, 851 |
| TaqI | TCGA | 2 cut(s) | 250, 732 |
| TasI | AATT | 3 cut(s) | 450, 682, 841 |
| TatI | WGTACW | 1 cut(s) | 280 |
| TauI | GCSGC | 1 cut(s) | 697 |
| TfiI | GAWTC | 4 cut(s) | 223, 252, 568, 678 |
| Tru1I | TTAA | 3 cut(s) | 513, 672, 788 |
| Tru9I | TTAA | 3 cut(s) | 513, 672, 788 |
| TscAI | CASTG | 2 cut(s) | 18, 856 |
| TseFI | GTSAC | 2 cut(s) | 346, 417 |
| TseI | GCWGC | 4 cut(s) | 88, 109, 203, 233 |
| Tsp45I | GTSAC | 2 cut(s) | 346, 417 |
| TspDTI | ATGAA | 4 cut(s) | 63, 65, 90, 394 |
| TspGWI | ACGGA | 1 cut(s) | 827 |
| TspRI | CASTG | 2 cut(s) | 18, 856 |
| Tth111I | GACNNNGTC | 1 cut(s) | 398 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 866 |
| VpaK11BI | GGWCC | 1 cut(s) | 502 |
| XapI | RAATTY | 1 cut(s) | 450 |
| XceI | RCATGY | 4 cut(s) | 478, 701, 740, 754 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 767, 906 |
| XmnI | GAANNNNTTC | 1 cut(s) | 72 |