MD04G1141100.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000357
Physical Location & Seq
Reverse (-)
22968897 .. 22970834
1938 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1141100.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGGCTTCTCTAGAGGAACCAAAAGGCCTTCCTGACATTCCTCTTGATGATGAAGCAAGGAAAGAAGAAGAGAGCTACTGCCATGCACTGCAGCTGGTGGTTTCTTCAGTGCTGTCCTTCTCCATGCAGTCAGCAATTGAGCTTGGCGTTTTTGACATCATAGCAAAAGAGGGTCAAAATGCCAAGCTCTCTTCATCTAAGATCGCAGCTCACATCGGAACCAAGACCCCTGACGGACCCATGATGCTAGATCGCCTTCTAGCTGTCCTGGCCAGCAACTCCGTGCTCGACTGCACAGTTGTTGACGGTAAACTTGACAAGTGTTTCCGGAGGCTCTACGGCCTTACCCCTGTGTCCAAGCACTTTGTGACTAATGAAGATGGTGTTTCCTTAGCCCCTGTGTTGACTTTGGTTCAAGACGAGGCCTTCTTAAAAGGCTGGCGTCAGGTGAAAGATGCAGTTATTGAAGGAGAAATTGCCTTTGACAGAGCTCATGGCATGCACCACTTTCAGTATCCAAGTGTCGACCATAGGTTTAATGAAATATTCAACAAGGCAATGTTCAACCATAGCACCCTAGTTATGAAGAGAATTCTCAAGCTCTACAAAGGTTTTGAGCACGTTACGCAGCTTGTTGATGTTGGTGGTAATTTGGGAGGGGCAATTAATCTAATCACTTCTAAATATCCACATATTAAGGGCATCAATTTTGACTTACCTCATGTTATAAAACATGCCTACTCTTATCCTGGTGTTGAAAATGTAGGAGGAGACATGTTTGAAAGTATTCCAAATGGGGATGCCATTTTTTTGAAGAACATACTTCATGACTGGTTGGATAAAGATTGCATAAAGTTATTGAAAAATTGTTACAATGCAATTCCAGACAATGGAAAAGTGATAGTGGTGGAGGCACTTCTCCCAATTAAGCCGGATTCTAACCTATCTGTGAGGACCAACGGCCAACTTGATCTGCATATGATGACACAAAGCCCGGGAGGGATGGAGAGGAGCCAAGAAGAATTCATGGCCTTAGCAACTGCTGCTGGATTTAGTGGCATCAGATATGAATGTTTCACTGCTAATCTTTGGATCATGGAATTCTACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01058 ko01100 ko01110 map00940 map01058 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

371

Amino Acids

41.11

Weight (kDa)

5.78

Isoelectric Point (pI)

34.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 31 - 124 2.8e-17 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 147 - 352 2.2e-64 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000357)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17810 FvH4_7g17811 FvH4_7g17820 FvH4_7g17830
malus_domestica MD00G1100700.v1.1 MD01G1089600.v1.1 MD01G1089800.v1.1 MD01G1090400.v1.1 MD01G1090500.v1.1 MD01G1090800.v1.1 MD04G1141100.v1.1 MD07G1161000.v1.1 MD07G1161100.v1.1 MD10G1030000.v1.1 MD10G1030200.v1.1 MD15G1409200.v1.1 MD15G1409700.v1.1
prunus_persica Prupe.2G199100_v2.0.a1 Prupe.2G199100_v2.0.a1 Prupe.2G199300_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199500_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199800_v2.0.a1 Prupe.2G200100_v2.0.a1 Prupe.2G200100_v2.0.a1
pyrus_communis pycom01g11360 pycom01g11370 pycom01g11400 pycom01g11440 pycom01g11480 pycom01g11490 pycom07g15690 pycom08g12100
rosa_chinensis RchiOBHm_Chr1g0317931 RchiOBHm_Chr1g0361211 RchiOBHm_Chr1g0361221 RchiOBHm_Chr1g0361241 RchiOBHm_Chr1g0367691 RchiOBHm_Chr4g0390171 RchiOBHm_Chr4g0396271 RchiOBHm_Chr6g0286421
rosa_laevigata RLG00000002555 RLG00000009555 RLG00000027275 RLG00000027731 RLG00000027732 RLG00000027734 RLG00000027738 RLG00000030575
rosa_multiflora Rmu_co8470451.1_g000001 Rmu_sc0000250.1_g000010 Rmu_sc0010379.1_g000006 Rmu_sc0011132.1_g000005 Rmu_sc0011132.1_g000007 Rmu_sc0038028.1_g000001 Rmu_ssc0000008.1_g000043 Rmu_ssc0000234.1_g000002 Rmu_ssc0000418.1_g000001
rosa_roxburghii Rroxscaffold_2G00116370 Rroxscaffold_4G00289300 Rroxscaffold_4G00295130 Rroxscaffold_4G00295140 Rroxscaffold_4G00295160 Rroxscaffold_4G00295190 Rroxscaffold_4G00295220 Rroxscaffold_4G00330100 Rroxscaffold_5G00341100
rosa_rugosa Rorug01G0287600 Rorug01G0287700 Rorug01G0336200 Rorug01G0336200 Rorug03G0350700
rosa_samantha Rh1AG030300 Rh1AG298500 Rh1AG298600 Rh1AG298700 Rh1AG298900 Rh1BG262400 Rh1BG262500 Rh1BG262800 Rh1BG304800 Rh1CG029000 Rh1CG279800 Rh1CG279900 Rh1CG280000 Rh1CG280100 Rh1CG280200 Rh1CG320500 Rh1DG041200 Rh1DG292700 Rh1DG292800 Rh1DG337100 Rh3DG107500 Rh4AG128100 Rh4BG024900 Rh4CG072700 Rh4DG063000 Rh6CG295900 Rh6DG287700
rosa_wichuraiana Rw1G002290 Rw1G026410 Rw1G026420 Rw1G026440 Rw4G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 728
AccB7I CCANNNNNTGG 1 cut(s) 890
AccI GTMKAC 1 cut(s) 525
AccIII TCCGGA 1 cut(s) 327
AclWI GGATC 1 cut(s) 1100
AcoI YGGCCR 2 cut(s) 270, 961
AcsI RAATTY 3 cut(s) 591, 1022, 1100
AcuI CTGAAG 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 442
AdeI CACNNNGTG 1 cut(s) 367
AfiI CCNNNNNNNGG 1 cut(s) 890
AflIII ACRYGT 1 cut(s) 774
AgsI TTSAA 8 cut(s) 416, 467, 550, 565, 758, 782, 814, 862
AjnI CCWGG 2 cut(s) 267, 748
AluBI AGCT 9 cut(s) 75, 94, 142, 187, 209, 263, 491, 601, 631
AluI AGCT 9 cut(s) 75, 94, 142, 187, 209, 263, 491, 601, 631
Alw21I GWGCWC 3 cut(s) 288, 493, 621
Alw26I GTCTC 1 cut(s) 765
AlwI GGATC 1 cut(s) 1100
Ama87I CYCGRG 1 cut(s) 994
Aor13HI TCCGGA 1 cut(s) 327
AoxI GGCC 6 cut(s) 25, 270, 340, 423, 961, 1029
ApeKI GCWGC 4 cut(s) 91, 206, 628, 1043
ApoI RAATTY 3 cut(s) 591, 1022, 1100
AseI ATTAAT 1 cut(s) 666
Asp700I GAANNNNTTC 1 cut(s) 786
AspS9I GGNCC 2 cut(s) 236, 954
AsuC2I CCSGG 2 cut(s) 995, 996
AsuHPI GGTGA 1 cut(s) 460
AvaI CYCGRG 1 cut(s) 994
AvaII GGWCC 2 cut(s) 236, 954
BalI TGGCCA 1 cut(s) 272
BanII GRGCYC 1 cut(s) 493
Bbv12I GWGCWC 3 cut(s) 288, 493, 621
BbvI GCAGC 4 cut(s) 103, 218, 640, 1030
BccI CCATC 2 cut(s) 374, 997
BceAI ACGGC 2 cut(s) 355, 976
BciT130I CCWGG 2 cut(s) 269, 750
BciVI GTATCC 1 cut(s) 525
BcnI CCSGG 2 cut(s) 995, 996
BcoDI GTCTC 1 cut(s) 765
BfaI CTAG 4 cut(s) 11, 248, 260, 578
BfmI CTRYAG 1 cut(s) 89
BfuI GTATCC 1 cut(s) 525
BisI GCNGC 4 cut(s) 92, 207, 629, 1044
BlsI GCNGC 4 cut(s) 93, 208, 630, 1045
Bme1390I CCNGG 4 cut(s) 269, 750, 995, 996
Bme18I GGWCC 2 cut(s) 236, 954
BmeT110I CYCGRG 1 cut(s) 994
BmgT120I GGNCC 2 cut(s) 236, 954
BmiI GGNNCC 4 cut(s) 18, 220, 238, 1013
BmrFI CCNGG 4 cut(s) 269, 750, 995, 996
BmsI GCATC 5 cut(s) 234, 445, 711, 790, 1068
Bpu10I CCTNAGC 2 cut(s) 391, 1033
BpuEI CTTGAG 1 cut(s) 581
BpuMI CCSGG 2 cut(s) 995, 996
BsaHI GRCGYC 1 cut(s) 442
BsaJI CCNNGG 1 cut(s) 994
BsaWI WCCGGW 1 cut(s) 327
Bsc4I CCNNNNNNNGG 1 cut(s) 890
Bse1I ACTGG 1 cut(s) 836
Bse3DI GCAATG 1 cut(s) 564
BseAI TCCGGA 1 cut(s) 327
BseBI CCWGG 2 cut(s) 269, 750
BseDI CCNNGG 1 cut(s) 994
BseGI GGATG 2 cut(s) 805, 1008
BseLI CCNNNNNNNGG 1 cut(s) 890
BseMI GCAATG 1 cut(s) 564
BseNI ACTGG 1 cut(s) 836
BseRI GAGGAG 2 cut(s) 783, 1024
BseXI GCAGC 4 cut(s) 103, 218, 640, 1030
BsgI GTGCAG 1 cut(s) 277
BshFI GGCC 6 cut(s) 27, 272, 342, 425, 963, 1031
BsiHKAI GWGCWC 3 cut(s) 288, 493, 621
BsiHKCI CYCGRG 1 cut(s) 994
BsiSI CCGG 3 cut(s) 328, 932, 995
BslI CCNNNNNNNGG 1 cut(s) 890
BsmAI GTCTC 1 cut(s) 765
BsnI GGCC 6 cut(s) 27, 272, 342, 425, 963, 1031
BsoBI CYCGRG 1 cut(s) 994
Bsp1286I GDGCHC 3 cut(s) 288, 493, 621
Bsp13I TCCGGA 1 cut(s) 327
Bsp143I GATC 4 cut(s) 201, 250, 970, 1092
BspANI GGCC 6 cut(s) 27, 272, 342, 425, 963, 1031
BspEI TCCGGA 1 cut(s) 327
BspHI TCATGA 1 cut(s) 826
BspLI GGNNCC 4 cut(s) 18, 220, 238, 1013
BspMAI CTGCAG 1 cut(s) 93
BspPI GGATC 1 cut(s) 1100
BsrDI GCAATG 1 cut(s) 564
BsrI ACTGG 1 cut(s) 836
BssECI CCNNGG 1 cut(s) 994
BssMI GATC 4 cut(s) 201, 250, 970, 1092
BssNI GRCGYC 1 cut(s) 442
Bst2UI CCWGG 2 cut(s) 269, 750
Bst4CI ACNGT 2 cut(s) 298, 308
Bst6I CTCTTC 3 cut(s) 63, 196, 581
BstACI GRCGYC 1 cut(s) 442
BstAPI GCANNNNNTGC 1 cut(s) 1043
BstC8I GCNNGC 3 cut(s) 274, 440, 500
BstDEI CTNAG 3 cut(s) 198, 391, 1033
BstF5I GGATG 2 cut(s) 805, 1008
BstKTI GATC 4 cut(s) 204, 253, 973, 1095
BstMAI GTCTC 1 cut(s) 765
BstMBI GATC 4 cut(s) 201, 250, 970, 1092
BstMWI GCNNNNNNNGC 3 cut(s) 269, 625, 1043
BstNI CCWGG 2 cut(s) 269, 750
BstNSI RCATGY 3 cut(s) 502, 737, 778
BstSCI CCNGG 4 cut(s) 267, 748, 993, 994
BstSFI CTRYAG 1 cut(s) 89
BstV1I GCAGC 4 cut(s) 103, 218, 640, 1030
BsuI GTATCC 1 cut(s) 525
BsuRI GGCC 6 cut(s) 27, 272, 342, 425, 963, 1031
BtsCI GGATG 2 cut(s) 805, 1008
BtsI GCAGTG 2 cut(s) 86, 1077
BtsIMutI CAGTG 3 cut(s) 86, 114, 1077
Cac8I GCNNGC 3 cut(s) 274, 440, 500
CciI TCATGA 1 cut(s) 826
Cfr13I GGNCC 2 cut(s) 236, 954
Cfr9I CCCGGG 1 cut(s) 994
CseI GACGC 1 cut(s) 431
DdeI CTNAG 3 cut(s) 198, 391, 1033
DpnI GATC 4 cut(s) 203, 252, 972, 1094
DpnII GATC 4 cut(s) 201, 250, 970, 1092
DraIII CACNNNGTG 1 cut(s) 367
EaeI YGGCCR 2 cut(s) 270, 961
Eam1104I CTCTTC 3 cut(s) 63, 196, 581
EarI CTCTTC 3 cut(s) 63, 196, 581
Ecl136II GAGCTC 1 cut(s) 491
Eco147I AGGCCT 2 cut(s) 27, 425
Eco24I GRGCYC 1 cut(s) 493
Eco47I GGWCC 2 cut(s) 236, 954
Eco53kI GAGCTC 1 cut(s) 491
Eco57I CTGAAG 1 cut(s) 90
Eco88I CYCGRG 1 cut(s) 994
EcoICRI GAGCTC 1 cut(s) 491
EcoRI GAATTC 3 cut(s) 591, 1022, 1100
EcoRII CCWGG 2 cut(s) 267, 748
EcoT38I GRGCYC 1 cut(s) 493
FauNDI CATATG 1 cut(s) 978
FblI GTMKAC 1 cut(s) 525
Fnu4HI GCNGC 4 cut(s) 92, 207, 629, 1044
FokI GGATG 2 cut(s) 812, 1015
FriOI GRGCYC 1 cut(s) 493
Fsp4HI GCNGC 4 cut(s) 92, 207, 629, 1044
FspBI CTAG 4 cut(s) 11, 248, 260, 578
GluI GCNGC 4 cut(s) 92, 207, 629, 1044
HaeIII GGCC 6 cut(s) 27, 272, 342, 425, 963, 1031
HapII CCGG 3 cut(s) 328, 932, 995
HgaI GACGC 1 cut(s) 431
Hin1I GRCGYC 1 cut(s) 442
HincII GTYRAC 3 cut(s) 304, 405, 526
HindII GTYRAC 3 cut(s) 304, 405, 526
HinfI GANTC 1 cut(s) 935
HpaII CCGG 3 cut(s) 328, 932, 995
HphI GGTGA 1 cut(s) 460
Hpy166II GTNNAC 4 cut(s) 304, 311, 405, 526
Hpy188I TCNGA 2 cut(s) 218, 1064
Hpy188III TCNNGA 7 cut(s) 11, 32, 44, 328, 416, 827, 884
Hpy8I GTNNAC 4 cut(s) 304, 311, 405, 526
HpyAV CCTTC 5 cut(s) 38, 127, 266, 436, 461
HpyCH4III ACNGT 2 cut(s) 298, 308
HpyCH4IV ACGT 1 cut(s) 621
HpyCH4V TGCA 9 cut(s) 86, 91, 127, 294, 458, 502, 849, 878, 976
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 625, 1043
HpyF3I CTNAG 3 cut(s) 198, 391, 1033
HpySE526I ACGT 1 cut(s) 621
Hsp92I GRCGYC 1 cut(s) 442
Kpn2I TCCGGA 1 cut(s) 327
Kzo9I GATC 4 cut(s) 201, 250, 970, 1092
LmnI GCTCC 1 cut(s) 1011
Lsp1109I GCAGC 4 cut(s) 103, 218, 640, 1030
LweI GCATC 5 cut(s) 234, 445, 711, 790, 1068
MaeI CTAG 4 cut(s) 11, 248, 260, 578
MaeII ACGT 1 cut(s) 621
MaeIII GTNAC 3 cut(s) 367, 622, 869
MalI GATC 4 cut(s) 203, 252, 972, 1094
MboI GATC 4 cut(s) 201, 250, 970, 1092
MboII GAAGA 8 cut(s) 77, 80, 96, 183, 389, 598, 826, 1031
MfeI CAATTG 1 cut(s) 135
MhlI GDGCHC 3 cut(s) 288, 493, 621
MlsI TGGCCA 1 cut(s) 272
MluNI TGGCCA 1 cut(s) 272
MmeI TCCRAC 1 cut(s) 816
Mox20I TGGCCA 1 cut(s) 272
MroI TCCGGA 1 cut(s) 327
MroXI GAANNNNTTC 1 cut(s) 786
MscI TGGCCA 1 cut(s) 272
MseI TTAA 5 cut(s) 431, 537, 666, 696, 927
Msp20I TGGCCA 1 cut(s) 272
MspA1I CMGCKG 1 cut(s) 94
MspI CCGG 3 cut(s) 328, 932, 995
MspR9I CCNGG 4 cut(s) 269, 750, 995, 996
MunI CAATTG 1 cut(s) 135
MvaI CCWGG 2 cut(s) 269, 750
MwoI GCNNNNNNNGC 3 cut(s) 269, 625, 1043
NciI CCSGG 2 cut(s) 995, 996
NdeI CATATG 1 cut(s) 978
NdeII GATC 4 cut(s) 201, 250, 970, 1092
NlaIV GGNNCC 4 cut(s) 18, 220, 238, 1013
NmuCI GTSAC 1 cut(s) 367
NspI RCATGY 3 cut(s) 502, 737, 778
PaeI GCATGC 1 cut(s) 502
PagI TCATGA 1 cut(s) 826
PceI AGGCCT 2 cut(s) 27, 425
PciI ACATGT 1 cut(s) 774
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 1 cut(s) 935
PflMI CCANNNNNTGG 1 cut(s) 890
PkrI GCNGC 4 cut(s) 93, 208, 630, 1045
PscI ACATGT 1 cut(s) 774
PshBI ATTAAT 1 cut(s) 666
PsiI TTATAA 1 cut(s) 728
Psp124BI GAGCTC 1 cut(s) 493
Psp6I CCWGG 2 cut(s) 267, 748
PspGI CCWGG 2 cut(s) 267, 748
PspN4I GGNNCC 4 cut(s) 18, 220, 238, 1013
PspPI GGNCC 2 cut(s) 236, 954
PstI CTGCAG 1 cut(s) 93
PvuII CAGCTG 1 cut(s) 94
SacI GAGCTC 1 cut(s) 493
SalI GTCGAC 1 cut(s) 524
SaqAI TTAA 5 cut(s) 431, 537, 666, 696, 927
SatI GCNGC 4 cut(s) 92, 207, 629, 1044
Sau3AI GATC 4 cut(s) 201, 250, 970, 1092
Sau96I GGNCC 2 cut(s) 236, 954
ScrFI CCNGG 4 cut(s) 269, 750, 995, 996
SduI GDGCHC 3 cut(s) 288, 493, 621
SfaNI GCATC 5 cut(s) 234, 445, 711, 790, 1068
SfcI CTRYAG 1 cut(s) 89
SinI GGWCC 2 cut(s) 236, 954
SmaI CCCGGG 1 cut(s) 996
SmlI CTYRAG 1 cut(s) 596
SmoI CTYRAG 1 cut(s) 596
SphI GCATGC 1 cut(s) 502
SseBI AGGCCT 2 cut(s) 27, 425
SspI AATATT 1 cut(s) 546
SspMI CTAG 4 cut(s) 11, 248, 260, 578
SstI GAGCTC 1 cut(s) 493
StuI AGGCCT 2 cut(s) 27, 425
StyD4I CCNGG 4 cut(s) 267, 748, 993, 994
TaaI ACNGT 2 cut(s) 298, 308
TaiI ACGT 1 cut(s) 624
TaqI TCGA 2 cut(s) 288, 525
TfiI GAWTC 1 cut(s) 935
Tru1I TTAA 5 cut(s) 431, 537, 666, 696, 927
Tru9I TTAA 5 cut(s) 431, 537, 666, 696, 927
TscAI CASTG 3 cut(s) 93, 114, 1084
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 4 cut(s) 91, 206, 628, 1043
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 8 cut(s) 66, 183, 390, 555, 599, 815, 1015, 1083
TspGWI ACGGA 2 cut(s) 249, 271
TspMI CCCGGG 1 cut(s) 994
TspRI CASTG 3 cut(s) 93, 114, 1084
Van91I CCANNNNNTGG 1 cut(s) 890
VpaK11BI GGWCC 2 cut(s) 236, 954
VspI ATTAAT 1 cut(s) 666
XapI RAATTY 3 cut(s) 591, 1022, 1100
XbaI TCTAGA 1 cut(s) 10
XceI RCATGY 3 cut(s) 502, 737, 778
XmaI CCCGGG 1 cut(s) 994
XmiI GTMKAC 1 cut(s) 525
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 4 cut(s) 11, 248, 260, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.