MD01G1089800.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000357
Physical Location & Seq
Reverse (-)
20342364 .. 20343432
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1089800.v1.1.491

Sequence Viewer

Length: 555 bp
ATGTTCAACCACAGCACCATAGTTATGAAGAGAATTCTCAAGCTCTACAAAGGTTTTGAGCACGTTACGCAGCTTGTTGATATTGGTGGTAATTTGGGAGGGGCAATTAGTCTCATTACTTCTAAATATCCACATATTAAAGGCATCAATTTTGACTTACCTCATGTTATAAAACATGCCTCCTCTTATCCTGGTGTTGAAAATGTAGGAGGAGACATGTTTGAAAGTATTCCAAATGGGGATGCCATTTTTTTGAAGTTCATACTTCATGACTGGTTGGATAAAGACTGCATAAAGTTATTGAAAAATTGTTACAATGCAATTCCAGACAATGGAAAAGTGATCGTGGTGGAGGCACTTCTCCCAATTAAGCCAGATTCTAACCTATCTGTGAGGACCAACGGCCAACTTGATCTGCATATGATGACTCAAACCCCGGGAGGGATGGAGAGGAGCCAAGAAGAATTCATGGCCTTAGCAACTGCTTCTGGATTTAGTGGAATCAGATATGAATGTTTCACTGCTAATCTTTGGATCATGGAATTCTACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01058 ko01100 ko01110 map00940 map01058 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

185

Amino Acids

20.58

Weight (kDa)

6.37

Isoelectric Point (pI)

20.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 2 - 166 3.7e-53 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000357)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17810 FvH4_7g17811 FvH4_7g17820 FvH4_7g17830
malus_domestica MD00G1100700.v1.1 MD01G1089600.v1.1 MD01G1089800.v1.1 MD01G1090400.v1.1 MD01G1090500.v1.1 MD01G1090800.v1.1 MD04G1141100.v1.1 MD07G1161000.v1.1 MD07G1161100.v1.1 MD10G1030000.v1.1 MD10G1030200.v1.1 MD15G1409200.v1.1 MD15G1409700.v1.1
prunus_persica Prupe.2G199100_v2.0.a1 Prupe.2G199100_v2.0.a1 Prupe.2G199300_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199500_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199800_v2.0.a1 Prupe.2G200100_v2.0.a1 Prupe.2G200100_v2.0.a1
pyrus_communis pycom01g11360 pycom01g11370 pycom01g11400 pycom01g11440 pycom01g11480 pycom01g11490 pycom07g15690 pycom08g12100
rosa_chinensis RchiOBHm_Chr1g0317931 RchiOBHm_Chr1g0361211 RchiOBHm_Chr1g0361221 RchiOBHm_Chr1g0361241 RchiOBHm_Chr1g0367691 RchiOBHm_Chr4g0390171 RchiOBHm_Chr4g0396271 RchiOBHm_Chr6g0286421
rosa_laevigata RLG00000002555 RLG00000009555 RLG00000027275 RLG00000027731 RLG00000027732 RLG00000027734 RLG00000027738 RLG00000030575
rosa_multiflora Rmu_co8470451.1_g000001 Rmu_sc0000250.1_g000010 Rmu_sc0010379.1_g000006 Rmu_sc0011132.1_g000005 Rmu_sc0011132.1_g000007 Rmu_sc0038028.1_g000001 Rmu_ssc0000008.1_g000043 Rmu_ssc0000234.1_g000002 Rmu_ssc0000418.1_g000001
rosa_roxburghii Rroxscaffold_2G00116370 Rroxscaffold_4G00289300 Rroxscaffold_4G00295130 Rroxscaffold_4G00295140 Rroxscaffold_4G00295160 Rroxscaffold_4G00295190 Rroxscaffold_4G00295220 Rroxscaffold_4G00330100 Rroxscaffold_5G00341100
rosa_rugosa Rorug01G0287600 Rorug01G0287700 Rorug01G0336200 Rorug01G0336200 Rorug03G0350700
rosa_samantha Rh1AG030300 Rh1AG298500 Rh1AG298600 Rh1AG298700 Rh1AG298900 Rh1BG262400 Rh1BG262500 Rh1BG262800 Rh1BG304800 Rh1CG029000 Rh1CG279800 Rh1CG279900 Rh1CG280000 Rh1CG280100 Rh1CG280200 Rh1CG320500 Rh1DG041200 Rh1DG292700 Rh1DG292800 Rh1DG337100 Rh3DG107500 Rh4AG128100 Rh4BG024900 Rh4CG072700 Rh4DG063000 Rh6CG295900 Rh6DG287700
rosa_wichuraiana Rw1G002290 Rw1G026410 Rw1G026420 Rw1G026440 Rw4G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 170
AccB7I CCANNNNNTGG 1 cut(s) 332
AclWI GGATC 1 cut(s) 542
AcoI YGGCCR 1 cut(s) 403
AcsI RAATTY 3 cut(s) 33, 464, 542
AfiI CCNNNNNNNGG 2 cut(s) 332, 441
AflIII ACRYGT 1 cut(s) 216
AgsI TTSAA 5 cut(s) 7, 200, 224, 256, 304
AjnI CCWGG 1 cut(s) 190
AluBI AGCT 2 cut(s) 43, 73
AluI AGCT 2 cut(s) 43, 73
Alw21I GWGCWC 1 cut(s) 63
Alw26I GTCTC 2 cut(s) 116, 207
AlwI GGATC 1 cut(s) 542
Ama87I CYCGRG 1 cut(s) 436
AoxI GGCC 2 cut(s) 403, 471
ApeKI GCWGC 1 cut(s) 70
ApoI RAATTY 3 cut(s) 33, 464, 542
Asp700I GAANNNNTTC 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 396
AsuC2I CCSGG 2 cut(s) 437, 438
AvaI CYCGRG 1 cut(s) 436
AvaII GGWCC 1 cut(s) 396
Bbv12I GWGCWC 1 cut(s) 63
BbvI GCAGC 1 cut(s) 82
BccI CCATC 1 cut(s) 439
BceAI ACGGC 1 cut(s) 418
BciT130I CCWGG 1 cut(s) 192
BcnI CCSGG 2 cut(s) 437, 438
BcoDI GTCTC 2 cut(s) 116, 207
BisI GCNGC 1 cut(s) 71
BlsI GCNGC 1 cut(s) 72
Bme1390I CCNGG 3 cut(s) 192, 437, 438
Bme18I GGWCC 1 cut(s) 396
BmeT110I CYCGRG 1 cut(s) 436
BmgT120I GGNCC 1 cut(s) 396
BmiI GGNNCC 1 cut(s) 455
BmrFI CCNGG 3 cut(s) 192, 437, 438
BmsI GCATC 2 cut(s) 153, 232
Bpu10I CCTNAGC 1 cut(s) 475
BpuEI CTTGAG 1 cut(s) 23
BpuMI CCSGG 2 cut(s) 437, 438
BsaJI CCNNGG 2 cut(s) 435, 436
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 441
Bse1I ACTGG 1 cut(s) 278
BseBI CCWGG 1 cut(s) 192
BseDI CCNNGG 2 cut(s) 435, 436
BseGI GGATG 2 cut(s) 247, 450
BseLI CCNNNNNNNGG 2 cut(s) 332, 441
BseNI ACTGG 1 cut(s) 278
BseRI GAGGAG 3 cut(s) 172, 225, 466
BseXI GCAGC 1 cut(s) 82
BshFI GGCC 2 cut(s) 405, 473
BsiHKAI GWGCWC 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 436
BsiSI CCGG 1 cut(s) 437
BslI CCNNNNNNNGG 2 cut(s) 332, 441
BsmAI GTCTC 2 cut(s) 116, 207
BsnI GGCC 2 cut(s) 405, 473
BsoBI CYCGRG 1 cut(s) 436
Bsp1286I GDGCHC 1 cut(s) 63
Bsp143I GATC 3 cut(s) 342, 412, 534
BspANI GGCC 2 cut(s) 405, 473
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 1 cut(s) 455
BspPI GGATC 1 cut(s) 542
BsrI ACTGG 1 cut(s) 278
BssECI CCNNGG 2 cut(s) 435, 436
BssMI GATC 3 cut(s) 342, 412, 534
Bst2UI CCWGG 1 cut(s) 192
Bst6I CTCTTC 1 cut(s) 23
BstDEI CTNAG 1 cut(s) 475
BstF5I GGATG 2 cut(s) 247, 450
BstKTI GATC 3 cut(s) 345, 415, 537
BstMAI GTCTC 2 cut(s) 116, 207
BstMBI GATC 3 cut(s) 342, 412, 534
BstMWI GCNNNNNNNGC 1 cut(s) 67
BstNI CCWGG 1 cut(s) 192
BstNSI RCATGY 2 cut(s) 179, 220
BstSCI CCNGG 3 cut(s) 190, 435, 436
BstV1I GCAGC 1 cut(s) 82
BsuRI GGCC 2 cut(s) 405, 473
BtsCI GGATG 2 cut(s) 247, 450
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 1 cut(s) 519
CciI TCATGA 1 cut(s) 268
Cfr13I GGNCC 1 cut(s) 396
Cfr9I CCCGGG 1 cut(s) 436
CviAII CATG 6 cut(s) 164, 176, 217, 269, 469, 538
CviJI RGCY 6 cut(s) 43, 73, 373, 405, 456, 473
CviKI_1 RGCY 6 cut(s) 43, 73, 373, 405, 456, 473
DdeI CTNAG 1 cut(s) 475
DpnI GATC 3 cut(s) 344, 414, 536
DpnII GATC 3 cut(s) 342, 412, 534
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
Eco47I GGWCC 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 436
EcoRI GAATTC 3 cut(s) 33, 464, 542
EcoRII CCWGG 1 cut(s) 190
FaeI CATG 6 cut(s) 167, 179, 220, 272, 472, 541
FatI CATG 6 cut(s) 163, 175, 216, 268, 468, 537
FauNDI CATATG 1 cut(s) 420
Fnu4HI GCNGC 1 cut(s) 71
FokI GGATG 2 cut(s) 254, 457
Fsp4HI GCNGC 1 cut(s) 71
GluI GCNGC 1 cut(s) 71
HaeIII GGCC 2 cut(s) 405, 473
HapII CCGG 1 cut(s) 437
Hin1II CATG 6 cut(s) 167, 179, 220, 272, 472, 541
HinfI GANTC 3 cut(s) 377, 427, 501
HpaII CCGG 1 cut(s) 437
Hpy188I TCNGA 1 cut(s) 506
Hpy188III TCNNGA 3 cut(s) 269, 326, 489
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 3 cut(s) 291, 320, 418
HpyF10VI GCNNNNNNNGC 1 cut(s) 67
HpyF3I CTNAG 1 cut(s) 475
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 6 cut(s) 167, 179, 220, 272, 472, 541
Kzo9I GATC 3 cut(s) 342, 412, 534
LmnI GCTCC 1 cut(s) 453
LpnPI CCDG 7 cut(s) 177, 204, 259, 339, 387, 450, 474
Lsp1109I GCAGC 1 cut(s) 82
LweI GCATC 2 cut(s) 153, 232
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 2 cut(s) 64, 311
MalI GATC 3 cut(s) 344, 414, 536
MboI GATC 3 cut(s) 342, 412, 534
MboII GAAGA 2 cut(s) 40, 473
MhlI GDGCHC 1 cut(s) 63
MluCI AATT 9 cut(s) 33, 91, 105, 148, 307, 321, 366, 464, 542
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 1 cut(s) 258
MnlI CCTC 9 cut(s) 92, 171, 190, 193, 203, 346, 387, 434, 444
MroXI GAANNNNTTC 1 cut(s) 228
MseI TTAA 2 cut(s) 138, 369
MslI CAYNNNNRTG 1 cut(s) 23
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 3 cut(s) 192, 437, 438
MvaI CCWGG 1 cut(s) 192
MwoI GCNNNNNNNGC 1 cut(s) 67
NciI CCSGG 2 cut(s) 437, 438
NdeI CATATG 1 cut(s) 420
NdeII GATC 3 cut(s) 342, 412, 534
NlaIII CATG 6 cut(s) 167, 179, 220, 272, 472, 541
NlaIV GGNNCC 1 cut(s) 455
NspI RCATGY 2 cut(s) 179, 220
PagI TCATGA 1 cut(s) 268
PciI ACATGT 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 228
PfeI GAWTC 2 cut(s) 377, 501
PflMI CCANNNNNTGG 1 cut(s) 332
PkrI GCNGC 1 cut(s) 72
PleI GAGTC 1 cut(s) 421
PpsI GAGTC 1 cut(s) 421
PscI ACATGT 1 cut(s) 216
PsiI TTATAA 1 cut(s) 170
Psp6I CCWGG 1 cut(s) 190
PspGI CCWGG 1 cut(s) 190
PspN4I GGNNCC 1 cut(s) 455
PspPI GGNCC 1 cut(s) 396
RseI CAYNNNNRTG 1 cut(s) 23
SaqAI TTAA 2 cut(s) 138, 369
SatI GCNGC 1 cut(s) 71
Sau3AI GATC 3 cut(s) 342, 412, 534
Sau96I GGNCC 1 cut(s) 396
SchI GAGTC 1 cut(s) 421
ScrFI CCNGG 3 cut(s) 192, 437, 438
SduI GDGCHC 1 cut(s) 63
SetI ASST 6 cut(s) 45, 55, 66, 75, 163, 387
SfaNI GCATC 2 cut(s) 153, 232
SinI GGWCC 1 cut(s) 396
SmaI CCCGGG 1 cut(s) 438
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
Sse9I AATT 9 cut(s) 33, 91, 105, 148, 307, 321, 366, 464, 542
StyD4I CCNGG 3 cut(s) 190, 435, 436
TaiI ACGT 1 cut(s) 66
TasI AATT 9 cut(s) 33, 91, 105, 148, 307, 321, 366, 464, 542
TfiI GAWTC 2 cut(s) 377, 501
Tru1I TTAA 2 cut(s) 138, 369
Tru9I TTAA 2 cut(s) 138, 369
TscAI CASTG 1 cut(s) 526
TseI GCWGC 1 cut(s) 70
TspDTI ATGAA 5 cut(s) 41, 250, 257, 457, 525
TspMI CCCGGG 1 cut(s) 436
TspRI CASTG 1 cut(s) 526
Van91I CCANNNNNTGG 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 396
XapI RAATTY 3 cut(s) 33, 464, 542
XceI RCATGY 2 cut(s) 179, 220
XmaI CCCGGG 1 cut(s) 436
XmnI GAANNNNTTC 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.