MD15G1409200.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000357
Physical Location & Seq
Forward (+)
50896162 .. 50898295
2134 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1409200.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGGCCCTGCAGCTGGAAGAAGAGGAAAACTTCGGCTGTGCCATGCAGCTGGTGTTTTCTTCTGTGCTGTCCATGTCTATGCAATCAGCAATCGAGCTAGGCGTTTTAGACATCATAGCGAAAGCCGGTCCTGGTGCCAATCTGTCTTCATCAGAGATTGCAGCCCATATCGGCAGCGGCACCAGGAATTCTGAGGCACCGATGATGTTGGATCGTATTCTAAGGCTCCTAGCCAGTTACTCTATTCTCAGCTGCTCTGTCGTTGCTAATGAAGAAGATGGGTCTGATTCTCAGAGGCTCTACAGCCTTGGTCCTGTGTCCAACTACTTTGTGACTAATGAAGATGGTATTTCTTTCGGTCCCATGATGGCATTGATTCAAGACAAGGTCGTCCTAGACTCCTGGTCCCAACTGAAAGATGCAGTTGTTGAAGGAGGAATTCCATTTAACAGGGTCTATGGCAAGGGGTGTTTTGAGTACCTAGGTTTAGACCCCAGGTTTAATCAAGTTTTCAACACAGCAATGTTTAACCACACCACTATTGTCACCAACAAGATTCTTCATCTCTACAAGGGTTTTGAGAAAATTACCCAACTTGTTGATGTTGGTGGTGGTTTGGGAGTCACCATTAGTCTAATCACTTCTAAGCATCCCCATATTAAGGGTATCAATTATGACTTGCCTCATGTCATAAAACATGCCGCTTCATATCCTGGGGTAGAACATGTTGGAGGAGACATGTTTGCAAGTGTTCCATCTGGGGATGCCATTTTGTTGAAGTGGATACTTCACGATTGGAGTGACCAACACTGCCTAAAGCTGTTGGAAAATTGTTACAATGCTATTCCAGATTATGGAAAAGTGATTATTGTGGAAGCACTTCTTCCAGTGATGCCAGAGACTAGCACCGCCGTGAAGAGCACCTCCCTACTTGATGTGCTTATGCTGAGTCAAAACAGGGGAGGAAAGGAGCGGAGCCGAGAAGAGTTCATGACTTTGGCAACTGGTGCTGGATTTAGTGGCATTAAATATGAATGTTTTTTTTCCAGCTTTTGCGTGATGGAGATCTTTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01058 ko01100 ko01110 map00940 map01058 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

359

Amino Acids

39.11

Weight (kDa)

5.2

Isoelectric Point (pI)

33.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 15 - 112 6.3e-20 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 135 - 340 4.2e-67 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000357)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17810 FvH4_7g17811 FvH4_7g17820 FvH4_7g17830
malus_domestica MD00G1100700.v1.1 MD01G1089600.v1.1 MD01G1089800.v1.1 MD01G1090400.v1.1 MD01G1090500.v1.1 MD01G1090800.v1.1 MD04G1141100.v1.1 MD07G1161000.v1.1 MD07G1161100.v1.1 MD10G1030000.v1.1 MD10G1030200.v1.1 MD15G1409200.v1.1 MD15G1409700.v1.1
prunus_persica Prupe.2G199100_v2.0.a1 Prupe.2G199100_v2.0.a1 Prupe.2G199300_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199500_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199800_v2.0.a1 Prupe.2G200100_v2.0.a1 Prupe.2G200100_v2.0.a1
pyrus_communis pycom01g11360 pycom01g11370 pycom01g11400 pycom01g11440 pycom01g11480 pycom01g11490 pycom07g15690 pycom08g12100
rosa_chinensis RchiOBHm_Chr1g0317931 RchiOBHm_Chr1g0361211 RchiOBHm_Chr1g0361221 RchiOBHm_Chr1g0361241 RchiOBHm_Chr1g0367691 RchiOBHm_Chr4g0390171 RchiOBHm_Chr4g0396271 RchiOBHm_Chr6g0286421
rosa_laevigata RLG00000002555 RLG00000009555 RLG00000027275 RLG00000027731 RLG00000027732 RLG00000027734 RLG00000027738 RLG00000030575
rosa_multiflora Rmu_co8470451.1_g000001 Rmu_sc0000250.1_g000010 Rmu_sc0010379.1_g000006 Rmu_sc0011132.1_g000005 Rmu_sc0011132.1_g000007 Rmu_sc0038028.1_g000001 Rmu_ssc0000008.1_g000043 Rmu_ssc0000234.1_g000002 Rmu_ssc0000418.1_g000001
rosa_roxburghii Rroxscaffold_2G00116370 Rroxscaffold_4G00289300 Rroxscaffold_4G00295130 Rroxscaffold_4G00295140 Rroxscaffold_4G00295160 Rroxscaffold_4G00295190 Rroxscaffold_4G00295220 Rroxscaffold_4G00330100 Rroxscaffold_5G00341100
rosa_rugosa Rorug01G0287600 Rorug01G0287700 Rorug01G0336200 Rorug01G0336200 Rorug03G0350700
rosa_samantha Rh1AG030300 Rh1AG298500 Rh1AG298600 Rh1AG298700 Rh1AG298900 Rh1BG262400 Rh1BG262500 Rh1BG262800 Rh1BG304800 Rh1CG029000 Rh1CG279800 Rh1CG279900 Rh1CG280000 Rh1CG280100 Rh1CG280200 Rh1CG320500 Rh1DG041200 Rh1DG292700 Rh1DG292800 Rh1DG337100 Rh3DG107500 Rh4AG128100 Rh4BG024900 Rh4CG072700 Rh4DG063000 Rh6CG295900 Rh6DG287700
rosa_wichuraiana Rw1G002290 Rw1G026410 Rw1G026420 Rw1G026440 Rw4G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 389
AccB1I GGYRCC 3 cut(s) 134, 179, 196
AccB7I CCANNNNNTGG 1 cut(s) 854
AccBSI CCGCTC 1 cut(s) 973
AciI CCGC 4 cut(s) 177, 702, 909, 973
AclWI GGATC 1 cut(s) 219
AcsI RAATTY 2 cut(s) 187, 438
AfaI GTAC 1 cut(s) 479
AfiI CCNNNNNNNGG 3 cut(s) 13, 661, 854
AflIII ACRYGT 2 cut(s) 724, 738
AgsI TTSAA 4 cut(s) 380, 431, 514, 778
AhdI GACNNNNNGTC 1 cut(s) 403
AjnI CCWGG 5 cut(s) 130, 182, 401, 494, 712
AleI CACNNNNGTG 1 cut(s) 911
AluBI AGCT 6 cut(s) 13, 49, 97, 252, 820, 1050
AluI AGCT 6 cut(s) 13, 49, 97, 252, 820, 1050
Alw21I GWGCWC 1 cut(s) 923
Alw26I GTCTC 2 cut(s) 729, 893
AlwI GGATC 1 cut(s) 219
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 5 cut(s) 10, 46, 161, 174, 252
ApoI RAATTY 2 cut(s) 187, 438
Asp700I GAANNNNTTC 1 cut(s) 879
AspA2I CCTAGG 1 cut(s) 481
AspS9I GGNCC 5 cut(s) 4, 128, 311, 359, 405
AsuHPI GGTGA 2 cut(s) 538, 616
AvaII GGWCC 4 cut(s) 128, 311, 359, 405
AvrII CCTAGG 1 cut(s) 481
BanI GGYRCC 3 cut(s) 134, 179, 196
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 923
BbvI GCAGC 5 cut(s) 22, 58, 173, 186, 239
BccI CCATC 5 cut(s) 272, 338, 361, 763, 1054
BceAI ACGGC 1 cut(s) 896
BciT130I CCWGG 5 cut(s) 132, 184, 403, 496, 714
BciVI GTATCC 1 cut(s) 777
BcoDI GTCTC 2 cut(s) 729, 893
BfaI CTAG 5 cut(s) 98, 230, 395, 482, 903
BfmI CTRYAG 2 cut(s) 8, 301
BfuI GTATCC 1 cut(s) 777
BglII AGATCT 1 cut(s) 1065
BisI GCNGC 7 cut(s) 11, 47, 162, 175, 178, 253, 702
BlnI CCTAGG 1 cut(s) 481
BlsI GCNGC 7 cut(s) 12, 48, 163, 176, 179, 254, 703
Bme1390I CCNGG 5 cut(s) 132, 184, 403, 496, 714
Bme18I GGWCC 4 cut(s) 128, 311, 359, 405
BmeRI GACNNNNNGTC 1 cut(s) 403
BmgT120I GGNCC 5 cut(s) 4, 128, 311, 359, 405
BmiI GGNNCC 7 cut(s) 136, 181, 198, 227, 361, 407, 977
BmrFI CCNGG 5 cut(s) 132, 184, 403, 496, 714
BmsI GCATC 4 cut(s) 409, 658, 754, 882
BpiI GAAGAC 1 cut(s) 138
BsaBI GATNNNNATC 1 cut(s) 1064
BsaJI CCNNGG 4 cut(s) 307, 481, 494, 713
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 661, 854
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 3 cut(s) 234, 887, 1009
Bse3DI GCAATG 1 cut(s) 528
Bse8I GATNNNNATC 1 cut(s) 1064
BseBI CCWGG 5 cut(s) 132, 184, 403, 496, 714
BseDI CCNNGG 4 cut(s) 307, 481, 494, 713
BseGI GGATG 2 cut(s) 649, 769
BseJI GATNNNNATC 1 cut(s) 1064
BseLI CCNNNNNNNGG 3 cut(s) 13, 661, 854
BseMI GCAATG 1 cut(s) 528
BseMII CTCAG 4 cut(s) 183, 262, 305, 938
BseNI ACTGG 3 cut(s) 234, 887, 1009
BseRI GAGGAG 1 cut(s) 747
BseXI GCAGC 5 cut(s) 22, 58, 173, 186, 239
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 3 cut(s) 134, 179, 196
BsiHKAI GWGCWC 1 cut(s) 923
BsiSI CCGG 1 cut(s) 126
BslFI GGGAC 2 cut(s) 345, 391
BslI CCNNNNNNNGG 3 cut(s) 13, 661, 854
BsmAI GTCTC 2 cut(s) 729, 893
BsmFI GGGAC 2 cut(s) 345, 391
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 923
Bsp143I GATC 2 cut(s) 211, 1065
BspACI CCGC 4 cut(s) 177, 702, 909, 973
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 4 cut(s) 184, 261, 304, 939
BspHI TCATGA 1 cut(s) 990
BspLI GGNNCC 7 cut(s) 136, 181, 198, 227, 361, 407, 977
BspMAI CTGCAG 1 cut(s) 12
BspPI GGATC 1 cut(s) 219
BspQI GCTCTTC 1 cut(s) 911
BspT107I GGYRCC 3 cut(s) 134, 179, 196
BsrBI CCGCTC 1 cut(s) 973
BsrDI GCAATG 1 cut(s) 528
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 3 cut(s) 234, 887, 1009
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 4 cut(s) 307, 481, 494, 713
BssMI GATC 2 cut(s) 211, 1065
BssT1I CCWWGG 2 cut(s) 307, 481
Bst2UI CCWGG 5 cut(s) 132, 184, 403, 496, 714
Bst6I CTCTTC 3 cut(s) 15, 911, 978
BstAPI GCANNNNNTGC 1 cut(s) 1007
BstDEI CTNAG 6 cut(s) 192, 221, 248, 291, 645, 947
BstF5I GGATG 2 cut(s) 649, 769
BstKTI GATC 2 cut(s) 214, 1068
BstMAI GTCTC 2 cut(s) 729, 893
BstMBI GATC 2 cut(s) 211, 1065
BstMWI GCNNNNNNNGC 1 cut(s) 1007
BstNI CCWGG 5 cut(s) 132, 184, 403, 496, 714
BstNSI RCATGY 3 cut(s) 701, 728, 742
BstSCI CCNGG 5 cut(s) 130, 182, 401, 494, 712
BstSFI CTRYAG 2 cut(s) 8, 301
BstV1I GCAGC 5 cut(s) 22, 58, 173, 186, 239
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 1 cut(s) 1065
BstXI CCANNNNNNTGG 1 cut(s) 49
BstYI RGATCY 1 cut(s) 1065
BsuI GTATCC 1 cut(s) 777
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 2 cut(s) 649, 769
BtsI GCAGTG 1 cut(s) 808
BtsIMutI CAGTG 2 cut(s) 808, 894
CciI TCATGA 1 cut(s) 990
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 5 cut(s) 4, 128, 311, 359, 405
Csp6I GTAC 1 cut(s) 478
CviAII CATG 8 cut(s) 43, 73, 364, 686, 698, 725, 739, 991
CviQI GTAC 1 cut(s) 478
DdeI CTNAG 6 cut(s) 192, 221, 248, 291, 645, 947
DpnI GATC 2 cut(s) 213, 1067
DpnII GATC 2 cut(s) 211, 1065
DrdI GACNNNNNNGTC 1 cut(s) 389
DriI GACNNNNNGTC 1 cut(s) 403
DseDI GACNNNNNNGTC 1 cut(s) 389
Eam1104I CTCTTC 3 cut(s) 15, 911, 978
Eam1105I GACNNNNNGTC 1 cut(s) 403
EarI CTCTTC 3 cut(s) 15, 911, 978
Eco130I CCWWGG 2 cut(s) 307, 481
Eco47I GGWCC 4 cut(s) 128, 311, 359, 405
EcoRI GAATTC 2 cut(s) 187, 438
EcoRII CCWGG 5 cut(s) 130, 182, 401, 494, 712
EcoT14I CCWWGG 2 cut(s) 307, 481
ErhI CCWWGG 2 cut(s) 307, 481
FaeI CATG 8 cut(s) 46, 76, 367, 689, 701, 728, 742, 994
FalI AAGNNNNNCTT 2 cut(s) 867, 899
FaqI GGGAC 2 cut(s) 345, 391
FatI CATG 8 cut(s) 42, 72, 363, 685, 697, 724, 738, 990
Fnu4HI GCNGC 7 cut(s) 11, 47, 162, 175, 178, 253, 702
FokI GGATG 2 cut(s) 636, 776
Fsp4HI GCNGC 7 cut(s) 11, 47, 162, 175, 178, 253, 702
FspBI CTAG 5 cut(s) 98, 230, 395, 482, 903
GluI GCNGC 7 cut(s) 11, 47, 162, 175, 178, 253, 702
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 126
Hin1II CATG 8 cut(s) 46, 76, 367, 689, 701, 728, 742, 994
HinfI GANTC 6 cut(s) 287, 376, 398, 556, 621, 949
HpaII CCGG 1 cut(s) 126
HphI GGTGA 2 cut(s) 538, 616
Hpy188I TCNGA 4 cut(s) 154, 193, 286, 294
Hpy188III TCNNGA 4 cut(s) 380, 791, 848, 991
HpyAV CCTTC 1 cut(s) 425
HpyCH4V TGCA 6 cut(s) 10, 46, 82, 161, 422, 746
HpyF10VI GCNNNNNNNGC 1 cut(s) 1007
HpyF3I CTNAG 6 cut(s) 192, 221, 248, 291, 645, 947
Hsp92II CATG 8 cut(s) 46, 76, 367, 689, 701, 728, 742, 994
Kzo9I GATC 2 cut(s) 211, 1065
LguI GCTCTTC 1 cut(s) 911
LmnI GCTCC 3 cut(s) 231, 970, 975
Lsp1109I GCAGC 5 cut(s) 22, 58, 173, 186, 239
LweI GCATC 4 cut(s) 409, 658, 754, 882
MaeI CTAG 5 cut(s) 98, 230, 395, 482, 903
MaeIII GTNAC 6 cut(s) 236, 331, 544, 622, 800, 833
MalI GATC 2 cut(s) 213, 1067
MbiI CCGCTC 1 cut(s) 973
MboI GATC 2 cut(s) 211, 1065
MflI RGATCY 1 cut(s) 1065
MhlI GDGCHC 1 cut(s) 923
MluCI AATT 5 cut(s) 187, 438, 585, 670, 829
MlyI GAGTC 3 cut(s) 392, 630, 958
MmeI TCCRAC 4 cut(s) 189, 345, 709, 804
MnlI CCTC 8 cut(s) 16, 187, 288, 428, 693, 725, 934, 956
MroXI GAANNNNTTC 1 cut(s) 879
MseI TTAA 6 cut(s) 447, 501, 528, 660, 1026, 1071
MslI CAYNNNNRTG 3 cut(s) 77, 521, 911
MspA1I CMGCKG 4 cut(s) 13, 49, 177, 252
MspI CCGG 1 cut(s) 126
MspR9I CCNGG 5 cut(s) 132, 184, 403, 496, 714
MvaI CCWGG 5 cut(s) 132, 184, 403, 496, 714
MwoI GCNNNNNNNGC 1 cut(s) 1007
NdeII GATC 2 cut(s) 211, 1065
NlaIII CATG 8 cut(s) 46, 76, 367, 689, 701, 728, 742, 994
NlaIV GGNNCC 7 cut(s) 136, 181, 198, 227, 361, 407, 977
NmeAIII GCCGAG 1 cut(s) 1004
NmuCI GTSAC 4 cut(s) 331, 544, 622, 800
NspI RCATGY 3 cut(s) 701, 728, 742
OliI CACNNNNGTG 1 cut(s) 911
PagI TCATGA 1 cut(s) 990
PciI ACATGT 2 cut(s) 724, 738
PciSI GCTCTTC 1 cut(s) 911
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PdmI GAANNNNTTC 1 cut(s) 879
PfeI GAWTC 3 cut(s) 287, 376, 556
PflFI GACNNNGTC 1 cut(s) 386
PflMI CCANNNNNTGG 1 cut(s) 854
PkrI GCNGC 7 cut(s) 12, 48, 163, 176, 179, 254, 703
PleI GAGTC 3 cut(s) 392, 629, 957
PpsI GAGTC 3 cut(s) 392, 629, 957
PscI ACATGT 2 cut(s) 724, 738
Psp6I CCWGG 5 cut(s) 130, 182, 401, 494, 712
PspGI CCWGG 5 cut(s) 130, 182, 401, 494, 712
PspN4I GGNNCC 7 cut(s) 136, 181, 198, 227, 361, 407, 977
PspPI GGNCC 5 cut(s) 4, 128, 311, 359, 405
PstI CTGCAG 1 cut(s) 12
PsuI RGATCY 1 cut(s) 1065
PsyI GACNNNGTC 1 cut(s) 386
PvuII CAGCTG 3 cut(s) 13, 49, 252
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
RseI CAYNNNNRTG 3 cut(s) 77, 521, 911
SapI GCTCTTC 1 cut(s) 911
SaqAI TTAA 6 cut(s) 447, 501, 528, 660, 1026, 1071
SatI GCNGC 7 cut(s) 11, 47, 162, 175, 178, 253, 702
Sau3AI GATC 2 cut(s) 211, 1065
Sau96I GGNCC 5 cut(s) 4, 128, 311, 359, 405
SchI GAGTC 3 cut(s) 392, 630, 958
ScrFI CCNGG 5 cut(s) 132, 184, 403, 496, 714
SduI GDGCHC 1 cut(s) 923
SfaNI GCATC 4 cut(s) 409, 658, 754, 882
SfcI CTRYAG 2 cut(s) 8, 301
SinI GGWCC 4 cut(s) 128, 311, 359, 405
SmiMI CAYNNNNRTG 3 cut(s) 77, 521, 911
Sse9I AATT 5 cut(s) 187, 438, 585, 670, 829
SsiI CCGC 4 cut(s) 177, 702, 909, 973
SspMI CTAG 5 cut(s) 98, 230, 395, 482, 903
StyD4I CCNGG 5 cut(s) 130, 182, 401, 494, 712
StyI CCWWGG 2 cut(s) 307, 481
TaqI TCGA 1 cut(s) 93
TaqII GACCGA 1 cut(s) 347
TasI AATT 5 cut(s) 187, 438, 585, 670, 829
TauI GCSGC 2 cut(s) 180, 704
TfiI GAWTC 3 cut(s) 287, 376, 556
Tru1I TTAA 6 cut(s) 447, 501, 528, 660, 1026, 1071
Tru9I TTAA 6 cut(s) 447, 501, 528, 660, 1026, 1071
TscAI CASTG 2 cut(s) 815, 894
TseFI GTSAC 4 cut(s) 331, 544, 622, 800
TseI GCWGC 5 cut(s) 10, 46, 161, 174, 252
Tsp45I GTSAC 4 cut(s) 331, 544, 622, 800
TspDTI ATGAA 7 cut(s) 138, 285, 354, 551, 696, 979, 1047
TspRI CASTG 2 cut(s) 815, 894
Tth111I GACNNNGTC 1 cut(s) 386
Van91I CCANNNNNTGG 1 cut(s) 854
VpaK11BI GGWCC 4 cut(s) 128, 311, 359, 405
XapI RAATTY 2 cut(s) 187, 438
XceI RCATGY 3 cut(s) 701, 728, 742
XmaJI CCTAGG 1 cut(s) 481
XmnI GAANNNNTTC 1 cut(s) 879
XspI CTAG 5 cut(s) 98, 230, 395, 482, 903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.