Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 401 |
| AccB1I | GGYRCC | 3 cut(s) | 146, 191, 208 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 866 |
| AccBSI | CCGCTC | 1 cut(s) | 985 |
| AciI | CCGC | 3 cut(s) | 189, 714, 985 |
| AclI | AACGTT | 1 cut(s) | 1062 |
| AclWI | GGATC | 1 cut(s) | 231 |
| AcsI | RAATTY | 2 cut(s) | 199, 450 |
| AfaI | GTAC | 1 cut(s) | 491 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 25, 673, 866 |
| AflIII | ACRYGT | 1 cut(s) | 736 |
| AgsI | TTSAA | 3 cut(s) | 443, 526, 790 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 415 |
| AjnI | CCWGG | 5 cut(s) | 142, 194, 413, 506, 724 |
| AluBI | AGCT | 5 cut(s) | 25, 61, 109, 137, 264 |
| AluI | AGCT | 5 cut(s) | 25, 61, 109, 137, 264 |
| Alw26I | GTCTC | 3 cut(s) | 486, 741, 905 |
| AlwI | GGATC | 1 cut(s) | 231 |
| AoxI | GGCC | 1 cut(s) | 15 |
| ApeKI | GCWGC | 4 cut(s) | 22, 58, 173, 264 |
| ApoI | RAATTY | 2 cut(s) | 199, 450 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 891 |
| AspLEI | GCGC | 1 cut(s) | 391 |
| AspS9I | GGNCC | 6 cut(s) | 16, 140, 323, 371, 417, 466 |
| AsuC2I | CCSGG | 1 cut(s) | 925 |
| AsuHPI | GGTGA | 1 cut(s) | 1081 |
| AvaII | GGWCC | 5 cut(s) | 140, 323, 371, 417, 466 |
| BanI | GGYRCC | 3 cut(s) | 146, 191, 208 |
| BbsI | GAAGAC | 2 cut(s) | 150, 935 |
| BbvI | GCAGC | 4 cut(s) | 34, 70, 185, 251 |
| BccI | CCATC | 5 cut(s) | 284, 350, 775, 860, 1066 |
| BciT130I | CCWGG | 5 cut(s) | 144, 196, 415, 508, 726 |
| BcnI | CCSGG | 1 cut(s) | 925 |
| BcoDI | GTCTC | 3 cut(s) | 486, 741, 905 |
| BfaI | CTAG | 4 cut(s) | 110, 242, 407, 915 |
| BfmI | CTRYAG | 2 cut(s) | 20, 313 |
| BglII | AGATCT | 1 cut(s) | 1077 |
| BisI | GCNGC | 6 cut(s) | 23, 59, 174, 190, 265, 714 |
| BlsI | GCNGC | 6 cut(s) | 24, 60, 175, 191, 266, 715 |
| Bme1390I | CCNGG | 6 cut(s) | 144, 196, 415, 508, 726, 925 |
| Bme18I | GGWCC | 5 cut(s) | 140, 323, 371, 417, 466 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 415 |
| BmgT120I | GGNCC | 6 cut(s) | 16, 140, 323, 371, 417, 466 |
| BmiI | GGNNCC | 8 cut(s) | 148, 193, 210, 239, 373, 419, 467, 989 |
| BmrFI | CCNGG | 6 cut(s) | 144, 196, 415, 508, 726, 925 |
| BmsI | GCATC | 4 cut(s) | 421, 670, 766, 894 |
| BpiI | GAAGAC | 2 cut(s) | 150, 935 |
| BpuMI | CCSGG | 1 cut(s) | 925 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1076 |
| BsaJI | CCNNGG | 4 cut(s) | 319, 469, 506, 725 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 25, 673, 866 |
| Bse1I | ACTGG | 3 cut(s) | 246, 899, 1021 |
| Bse3DI | GCAATG | 1 cut(s) | 540 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1076 |
| BseBI | CCWGG | 5 cut(s) | 144, 196, 415, 508, 726 |
| BseDI | CCNNGG | 4 cut(s) | 319, 469, 506, 725 |
| BseGI | GGATG | 2 cut(s) | 661, 781 |
| BseJI | GATNNNNATC | 1 cut(s) | 1076 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 25, 673, 866 |
| BseMI | GCAATG | 1 cut(s) | 540 |
| BseMII | CTCAG | 3 cut(s) | 195, 274, 317 |
| BseNI | ACTGG | 3 cut(s) | 246, 899, 1021 |
| BseRI | GAGGAG | 1 cut(s) | 759 |
| BseXI | GCAGC | 4 cut(s) | 34, 70, 185, 251 |
| BshFI | GGCC | 1 cut(s) | 17 |
| BshNI | GGYRCC | 3 cut(s) | 146, 191, 208 |
| BsiSI | CCGG | 1 cut(s) | 924 |
| BslFI | GGGAC | 2 cut(s) | 357, 403 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 25, 673, 866 |
| BsmAI | GTCTC | 3 cut(s) | 486, 741, 905 |
| BsmFI | GGGAC | 2 cut(s) | 357, 403 |
| BsnI | GGCC | 1 cut(s) | 17 |
| Bsp143I | GATC | 2 cut(s) | 223, 1077 |
| Bsp19I | CCATGG | 1 cut(s) | 469 |
| BspACI | CCGC | 3 cut(s) | 189, 714, 985 |
| BspANI | GGCC | 1 cut(s) | 17 |
| BspCNI | CTCAG | 3 cut(s) | 196, 273, 316 |
| BspHI | TCATGA | 3 cut(s) | 484, 558, 1002 |
| BspLI | GGNNCC | 8 cut(s) | 148, 193, 210, 239, 373, 419, 467, 989 |
| BspMAI | CTGCAG | 1 cut(s) | 24 |
| BspPI | GGATC | 1 cut(s) | 231 |
| BspT107I | GGYRCC | 3 cut(s) | 146, 191, 208 |
| BsrBI | CCGCTC | 1 cut(s) | 985 |
| BsrDI | GCAATG | 1 cut(s) | 540 |
| BsrI | ACTGG | 3 cut(s) | 246, 899, 1021 |
| BssECI | CCNNGG | 4 cut(s) | 319, 469, 506, 725 |
| BssMI | GATC | 2 cut(s) | 223, 1077 |
| BssT1I | CCWWGG | 2 cut(s) | 319, 469 |
| Bst2UI | CCWGG | 5 cut(s) | 144, 196, 415, 508, 726 |
| Bst4CI | ACNGT | 1 cut(s) | 8 |
| Bst6I | CTCTTC | 2 cut(s) | 27, 990 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1019 |
| BstC8I | GCNNGC | 2 cut(s) | 476, 756 |
| BstDEI | CTNAG | 5 cut(s) | 204, 233, 260, 303, 657 |
| BstDSI | CCRYGG | 1 cut(s) | 469 |
| BstF5I | GGATG | 2 cut(s) | 661, 781 |
| BstHHI | GCGC | 1 cut(s) | 391 |
| BstKTI | GATC | 2 cut(s) | 226, 1080 |
| BstMAI | GTCTC | 3 cut(s) | 486, 741, 905 |
| BstMBI | GATC | 2 cut(s) | 223, 1077 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1019 |
| BstNI | CCWGG | 5 cut(s) | 144, 196, 415, 508, 726 |
| BstNSI | RCATGY | 3 cut(s) | 713, 740, 754 |
| BstSCI | CCNGG | 6 cut(s) | 142, 194, 413, 506, 724, 923 |
| BstSFI | CTRYAG | 2 cut(s) | 20, 313 |
| BstV1I | GCAGC | 4 cut(s) | 34, 70, 185, 251 |
| BstV2I | GAAGAC | 2 cut(s) | 150, 935 |
| BstX2I | RGATCY | 1 cut(s) | 1077 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 61 |
| BstYI | RGATCY | 1 cut(s) | 1077 |
| BsuRI | GGCC | 1 cut(s) | 17 |
| BtgI | CCRYGG | 1 cut(s) | 469 |
| BtsCI | GGATG | 2 cut(s) | 661, 781 |
| BtsI | GCAGTG | 2 cut(s) | 820, 918 |
| BtsIMutI | CAGTG | 3 cut(s) | 820, 906, 918 |
| Cac8I | GCNNGC | 2 cut(s) | 476, 756 |
| CciI | TCATGA | 3 cut(s) | 484, 558, 1002 |
| CfoI | GCGC | 1 cut(s) | 391 |
| Cfr13I | GGNCC | 6 cut(s) | 16, 140, 323, 371, 417, 466 |
| Csp6I | GTAC | 1 cut(s) | 490 |
| CviQI | GTAC | 1 cut(s) | 490 |
| DdeI | CTNAG | 5 cut(s) | 204, 233, 260, 303, 657 |
| DpnI | GATC | 2 cut(s) | 225, 1079 |
| DpnII | GATC | 2 cut(s) | 223, 1077 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 401 |
| DriI | GACNNNNNGTC | 1 cut(s) | 415 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 401 |
| Eam1104I | CTCTTC | 2 cut(s) | 27, 990 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 415 |
| EarI | CTCTTC | 2 cut(s) | 27, 990 |
| Eco130I | CCWWGG | 2 cut(s) | 319, 469 |
| Eco47I | GGWCC | 5 cut(s) | 140, 323, 371, 417, 466 |
| EcoO109I | RGGNCCY | 1 cut(s) | 371 |
| EcoRI | GAATTC | 2 cut(s) | 199, 450 |
| EcoRII | CCWGG | 5 cut(s) | 142, 194, 413, 506, 724 |
| EcoT14I | CCWWGG | 2 cut(s) | 319, 469 |
| ErhI | CCWWGG | 2 cut(s) | 319, 469 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 879, 911 |
| FaqI | GGGAC | 2 cut(s) | 357, 403 |
| Fnu4HI | GCNGC | 6 cut(s) | 23, 59, 174, 190, 265, 714 |
| FokI | GGATG | 2 cut(s) | 648, 788 |
| Fsp4HI | GCNGC | 6 cut(s) | 23, 59, 174, 190, 265, 714 |
| FspBI | CTAG | 4 cut(s) | 110, 242, 407, 915 |
| GlaI | GCGC | 1 cut(s) | 390 |
| GluI | GCNGC | 6 cut(s) | 23, 59, 174, 190, 265, 714 |
| HaeIII | GGCC | 1 cut(s) | 17 |
| HapII | CCGG | 1 cut(s) | 924 |
| HhaI | GCGC | 1 cut(s) | 391 |
| Hin6I | GCGC | 1 cut(s) | 389 |
| HinP1I | GCGC | 1 cut(s) | 389 |
| HinfI | GANTC | 5 cut(s) | 299, 410, 568, 633, 961 |
| HpaII | CCGG | 1 cut(s) | 924 |
| HphI | GGTGA | 1 cut(s) | 1081 |
| Hpy166II | GTNNAC | 1 cut(s) | 492 |
| Hpy188I | TCNGA | 4 cut(s) | 166, 205, 298, 306 |
| Hpy188III | TCNNGA | 4 cut(s) | 485, 559, 860, 1003 |
| Hpy8I | GTNNAC | 1 cut(s) | 492 |
| HpyAV | CCTTC | 2 cut(s) | 437, 784 |
| HpyCH4III | ACNGT | 1 cut(s) | 8 |
| HpyCH4IV | ACGT | 1 cut(s) | 1062 |
| HpyCH4V | TGCA | 8 cut(s) | 22, 58, 94, 173, 383, 434, 758, 1059 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1019 |
| HpyF3I | CTNAG | 5 cut(s) | 204, 233, 260, 303, 657 |
| HpySE526I | ACGT | 1 cut(s) | 1062 |
| HspAI | GCGC | 1 cut(s) | 389 |
| Kzo9I | GATC | 2 cut(s) | 223, 1077 |
| LmnI | GCTCC | 3 cut(s) | 243, 982, 987 |
| Lsp1109I | GCAGC | 4 cut(s) | 34, 70, 185, 251 |
| LweI | GCATC | 4 cut(s) | 421, 670, 766, 894 |
| MaeI | CTAG | 4 cut(s) | 110, 242, 407, 915 |
| MaeII | ACGT | 1 cut(s) | 1062 |
| MaeIII | GTNAC | 5 cut(s) | 248, 343, 634, 812, 845 |
| MalI | GATC | 2 cut(s) | 225, 1079 |
| MbiI | CCGCTC | 1 cut(s) | 985 |
| MboI | GATC | 2 cut(s) | 223, 1077 |
| MfeI | CAATTG | 1 cut(s) | 805 |
| MflI | RGATCY | 1 cut(s) | 1077 |
| MluCI | AATT | 6 cut(s) | 199, 450, 597, 682, 805, 841 |
| MlyI | GAGTC | 3 cut(s) | 404, 642, 955 |
| MmeI | TCCRAC | 4 cut(s) | 201, 357, 721, 816 |
| MnlI | CCTC | 8 cut(s) | 28, 199, 300, 440, 705, 737, 946, 968 |
| MroXI | GAANNNNTTC | 1 cut(s) | 891 |
| MseI | TTAA | 5 cut(s) | 513, 540, 672, 1038, 1083 |
| MslI | CAYNNNNRTG | 2 cut(s) | 89, 533 |
| MspA1I | CMGCKG | 4 cut(s) | 25, 61, 189, 264 |
| MspI | CCGG | 1 cut(s) | 924 |
| MspR9I | CCNGG | 6 cut(s) | 144, 196, 415, 508, 726, 925 |
| MunI | CAATTG | 1 cut(s) | 805 |
| MvaI | CCWGG | 5 cut(s) | 144, 196, 415, 508, 726 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1019 |
| NciI | CCSGG | 1 cut(s) | 925 |
| NcoI | CCATGG | 1 cut(s) | 469 |
| NdeII | GATC | 2 cut(s) | 223, 1077 |
| NlaIV | GGNNCC | 8 cut(s) | 148, 193, 210, 239, 373, 419, 467, 989 |
| NmeAIII | GCCGAG | 1 cut(s) | 1016 |
| NmuCI | GTSAC | 4 cut(s) | 248, 343, 634, 812 |
| NspI | RCATGY | 3 cut(s) | 713, 740, 754 |
| PagI | TCATGA | 3 cut(s) | 484, 558, 1002 |
| PciI | ACATGT | 1 cut(s) | 736 |
| PdmI | GAANNNNTTC | 1 cut(s) | 891 |
| PfeI | GAWTC | 2 cut(s) | 299, 568 |
| PflFI | GACNNNGTC | 1 cut(s) | 398 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 866 |
| PkrI | GCNGC | 6 cut(s) | 24, 60, 175, 191, 266, 715 |
| PleI | GAGTC | 3 cut(s) | 404, 641, 955 |
| PpsI | GAGTC | 3 cut(s) | 404, 641, 955 |
| PpuMI | RGGWCCY | 1 cut(s) | 371 |
| PscI | ACATGT | 1 cut(s) | 736 |
| Psp1406I | AACGTT | 1 cut(s) | 1062 |
| Psp5II | RGGWCCY | 1 cut(s) | 371 |
| Psp6I | CCWGG | 5 cut(s) | 142, 194, 413, 506, 724 |
| PspGI | CCWGG | 5 cut(s) | 142, 194, 413, 506, 724 |
| PspN4I | GGNNCC | 8 cut(s) | 148, 193, 210, 239, 373, 419, 467, 989 |
| PspPI | GGNCC | 6 cut(s) | 16, 140, 323, 371, 417, 466 |
| PspPPI | RGGWCCY | 1 cut(s) | 371 |
| PstI | CTGCAG | 1 cut(s) | 24 |
| PsuI | RGATCY | 1 cut(s) | 1077 |
| PsyI | GACNNNGTC | 1 cut(s) | 398 |
| PvuII | CAGCTG | 3 cut(s) | 25, 61, 264 |
| RsaI | GTAC | 1 cut(s) | 491 |
| RsaNI | GTAC | 1 cut(s) | 490 |
| RseI | CAYNNNNRTG | 2 cut(s) | 89, 533 |
| SaqAI | TTAA | 5 cut(s) | 513, 540, 672, 1038, 1083 |
| SatI | GCNGC | 6 cut(s) | 23, 59, 174, 190, 265, 714 |
| Sau3AI | GATC | 2 cut(s) | 223, 1077 |
| Sau96I | GGNCC | 6 cut(s) | 16, 140, 323, 371, 417, 466 |
| SchI | GAGTC | 3 cut(s) | 404, 642, 955 |
| ScrFI | CCNGG | 6 cut(s) | 144, 196, 415, 508, 726, 925 |
| SfaNI | GCATC | 4 cut(s) | 421, 670, 766, 894 |
| SfcI | CTRYAG | 2 cut(s) | 20, 313 |
| SinI | GGWCC | 5 cut(s) | 140, 323, 371, 417, 466 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 89, 533 |
| Sse9I | AATT | 6 cut(s) | 199, 450, 597, 682, 805, 841 |
| SsiI | CCGC | 3 cut(s) | 189, 714, 985 |
| SspMI | CTAG | 4 cut(s) | 110, 242, 407, 915 |
| StyD4I | CCNGG | 6 cut(s) | 142, 194, 413, 506, 724, 923 |
| StyI | CCWWGG | 2 cut(s) | 319, 469 |
| TaaI | ACNGT | 1 cut(s) | 8 |
| TaiI | ACGT | 1 cut(s) | 1065 |
| TaqI | TCGA | 3 cut(s) | 105, 120, 183 |
| TasI | AATT | 6 cut(s) | 199, 450, 597, 682, 805, 841 |
| TatI | WGTACW | 1 cut(s) | 489 |
| TauI | GCSGC | 2 cut(s) | 192, 716 |
| TfiI | GAWTC | 2 cut(s) | 299, 568 |
| Tru1I | TTAA | 5 cut(s) | 513, 540, 672, 1038, 1083 |
| Tru9I | TTAA | 5 cut(s) | 513, 540, 672, 1038, 1083 |
| TscAI | CASTG | 3 cut(s) | 827, 906, 925 |
| TseFI | GTSAC | 4 cut(s) | 248, 343, 634, 812 |
| TseI | GCWGC | 4 cut(s) | 22, 58, 173, 264 |
| Tsp45I | GTSAC | 4 cut(s) | 248, 343, 634, 812 |
| TspDTI | ATGAA | 8 cut(s) | 150, 297, 366, 563, 575, 708, 991, 1059 |
| TspRI | CASTG | 3 cut(s) | 827, 906, 925 |
| Tth111I | GACNNNGTC | 1 cut(s) | 398 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 866 |
| VpaK11BI | GGWCC | 5 cut(s) | 140, 323, 371, 417, 466 |
| XapI | RAATTY | 2 cut(s) | 199, 450 |
| XceI | RCATGY | 3 cut(s) | 713, 740, 754 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 382 |
| XmnI | GAANNNNTTC | 1 cut(s) | 891 |
| XspI | CTAG | 4 cut(s) | 110, 242, 407, 915 |