Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 974 |
| AatII | GACGTC | 1 cut(s) | 35 |
| Acc36I | ACCTGC | 1 cut(s) | 974 |
| AccB1I | GGYRCC | 3 cut(s) | 206, 223, 401 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 896 |
| AciI | CCGC | 2 cut(s) | 164, 170 |
| AclWI | GGATC | 1 cut(s) | 246 |
| AcsI | RAATTY | 2 cut(s) | 597, 1028 |
| AcuI | CTGAAG | 1 cut(s) | 49 |
| AcyI | GRCGYC | 3 cut(s) | 32, 224, 402 |
| AdeI | CACNNNGTG | 1 cut(s) | 271 |
| AfaI | GTAC | 2 cut(s) | 365, 611 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 896 |
| AflII | CTTAAG | 1 cut(s) | 629 |
| AflIII | ACRYGT | 2 cut(s) | 766, 780 |
| AgsI | TTSAA | 3 cut(s) | 457, 556, 859 |
| AjnI | CCWGG | 2 cut(s) | 536, 937 |
| AleI | CACNNNNGTG | 1 cut(s) | 758 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 431, 463 |
| AluBI | AGCT | 9 cut(s) | 63, 69, 82, 130, 158, 197, 202, 444, 862 |
| AluI | AGCT | 9 cut(s) | 63, 69, 82, 130, 158, 197, 202, 444, 862 |
| Alw21I | GWGCWC | 5 cut(s) | 8, 276, 627, 852, 965 |
| Alw26I | GTCTC | 1 cut(s) | 771 |
| AlwI | GGATC | 1 cut(s) | 246 |
| Ama87I | CYCGRG | 1 cut(s) | 218 |
| AoxI | GGCC | 2 cut(s) | 320, 655 |
| ApeKI | GCWGC | 5 cut(s) | 79, 100, 194, 953, 1049 |
| ApoI | RAATTY | 2 cut(s) | 597, 1028 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 921 |
| AspLEI | GCGC | 2 cut(s) | 226, 404 |
| AspS9I | GGNCC | 4 cut(s) | 320, 496, 532, 942 |
| AsuC2I | CCSGG | 1 cut(s) | 1002 |
| AvaI | CYCGRG | 1 cut(s) | 218 |
| AvaII | GGWCC | 3 cut(s) | 496, 532, 942 |
| BanI | GGYRCC | 3 cut(s) | 206, 223, 401 |
| Bbv12I | GWGCWC | 5 cut(s) | 8, 276, 627, 852, 965 |
| BbvI | GCAGC | 5 cut(s) | 87, 91, 206, 940, 1036 |
| BccI | CCATC | 2 cut(s) | 380, 403 |
| BciT130I | CCWGG | 2 cut(s) | 538, 939 |
| BciVI | GTATCC | 2 cut(s) | 531, 819 |
| BclI | TGATCA | 1 cut(s) | 307 |
| BcnI | CCSGG | 1 cut(s) | 1002 |
| BcoDI | GTCTC | 1 cut(s) | 771 |
| BfaI | CTAG | 2 cut(s) | 83, 203 |
| BfoI | RGCGCY | 2 cut(s) | 227, 405 |
| BfrI | CTTAAG | 1 cut(s) | 629 |
| BfuAI | ACCTGC | 1 cut(s) | 974 |
| BfuI | GTATCC | 2 cut(s) | 531, 819 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 410 |
| BisI | GCNGC | 5 cut(s) | 80, 101, 195, 954, 1050 |
| BlpI | GCTNAGC | 1 cut(s) | 198 |
| BlsI | GCNGC | 5 cut(s) | 81, 102, 196, 955, 1051 |
| BmcAI | AGTACT | 1 cut(s) | 365 |
| Bme1390I | CCNGG | 3 cut(s) | 538, 939, 1002 |
| Bme18I | GGWCC | 3 cut(s) | 496, 532, 942 |
| BmeT110I | CYCGRG | 1 cut(s) | 218 |
| BmgT120I | GGNCC | 4 cut(s) | 320, 496, 532, 942 |
| BmiI | GGNNCC | 7 cut(s) | 208, 225, 403, 449, 497, 534, 943 |
| BmrFI | CCNGG | 3 cut(s) | 538, 939, 1002 |
| BmsI | GCATC | 2 cut(s) | 717, 796 |
| BpmI | CTGGAG | 1 cut(s) | 777 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 198 |
| BpuEI | CTTGAG | 1 cut(s) | 405 |
| BpuMI | CCSGG | 1 cut(s) | 1002 |
| BsaBI | GATNNNNATC | 1 cut(s) | 306 |
| BsaHI | GRCGYC | 3 cut(s) | 32, 224, 402 |
| BsaJI | CCNNGG | 6 cut(s) | 219, 499, 536, 658, 938, 1038 |
| BsaWI | WCCGGW | 1 cut(s) | 450 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 896 |
| Bse1I | ACTGG | 4 cut(s) | 23, 760, 842, 929 |
| Bse3DI | GCAATG | 1 cut(s) | 570 |
| Bse8I | GATNNNNATC | 1 cut(s) | 306 |
| BseBI | CCWGG | 2 cut(s) | 538, 939 |
| BseDI | CCNNGG | 6 cut(s) | 219, 499, 536, 658, 938, 1038 |
| BseGI | GGATG | 3 cut(s) | 182, 811, 982 |
| BseJI | GATNNNNATC | 1 cut(s) | 306 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 896 |
| BseMI | GCAATG | 1 cut(s) | 570 |
| BseMII | CTCAG | 2 cut(s) | 212, 262 |
| BseNI | ACTGG | 4 cut(s) | 23, 760, 842, 929 |
| BseRI | GAGGAG | 1 cut(s) | 789 |
| BseXI | GCAGC | 5 cut(s) | 87, 91, 206, 940, 1036 |
| BshFI | GGCC | 2 cut(s) | 322, 657 |
| BshNI | GGYRCC | 3 cut(s) | 206, 223, 401 |
| BsiHKAI | GWGCWC | 5 cut(s) | 8, 276, 627, 852, 965 |
| BsiHKCI | CYCGRG | 1 cut(s) | 218 |
| BsiSI | CCGG | 2 cut(s) | 451, 1001 |
| BslFI | GGGAC | 1 cut(s) | 955 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 896 |
| BsmAI | GTCTC | 1 cut(s) | 771 |
| BsmFI | GGGAC | 1 cut(s) | 955 |
| BsnI | GGCC | 2 cut(s) | 322, 657 |
| BsoBI | CYCGRG | 1 cut(s) | 218 |
| Bsp1286I | GDGCHC | 5 cut(s) | 8, 276, 627, 852, 965 |
| Bsp1407I | TGTACA | 1 cut(s) | 609 |
| Bsp143I | GATC | 3 cut(s) | 238, 307, 604 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 198 |
| Bsp19I | CCATGG | 1 cut(s) | 499 |
| BspACI | CCGC | 2 cut(s) | 164, 170 |
| BspANI | GGCC | 2 cut(s) | 322, 657 |
| BspCNI | CTCAG | 2 cut(s) | 211, 261 |
| BspHI | TCATGA | 1 cut(s) | 832 |
| BspLI | GGNNCC | 7 cut(s) | 208, 225, 403, 449, 497, 534, 943 |
| BspMI | ACCTGC | 1 cut(s) | 974 |
| BspPI | GGATC | 1 cut(s) | 246 |
| BspT107I | GGYRCC | 3 cut(s) | 206, 223, 401 |
| BspTI | CTTAAG | 1 cut(s) | 629 |
| BsrDI | GCAATG | 1 cut(s) | 570 |
| BsrGI | TGTACA | 1 cut(s) | 609 |
| BsrI | ACTGG | 4 cut(s) | 23, 760, 842, 929 |
| BssECI | CCNNGG | 6 cut(s) | 219, 499, 536, 658, 938, 1038 |
| BssMI | GATC | 3 cut(s) | 238, 307, 604 |
| BssNI | GRCGYC | 3 cut(s) | 32, 224, 402 |
| BssT1I | CCWWGG | 3 cut(s) | 499, 658, 1038 |
| Bst2UI | CCWGG | 2 cut(s) | 538, 939 |
| Bst4CI | ACNGT | 2 cut(s) | 14, 286 |
| Bst6I | CTCTTC | 1 cut(s) | 368 |
| BstACI | GRCGYC | 3 cut(s) | 32, 224, 402 |
| BstAFI | CTTAAG | 1 cut(s) | 629 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1049 |
| BstAUI | TGTACA | 1 cut(s) | 609 |
| BstDEI | CTNAG | 4 cut(s) | 18, 198, 248, 671 |
| BstDSI | CCRYGG | 1 cut(s) | 499 |
| BstF5I | GGATG | 3 cut(s) | 182, 811, 982 |
| BstH2I | RGCGCY | 2 cut(s) | 227, 405 |
| BstHHI | GCGC | 2 cut(s) | 226, 404 |
| BstKTI | GATC | 3 cut(s) | 241, 310, 607 |
| BstMAI | GTCTC | 1 cut(s) | 771 |
| BstMBI | GATC | 3 cut(s) | 238, 307, 604 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 410, 631, 1049 |
| BstNI | CCWGG | 2 cut(s) | 538, 939 |
| BstNSI | RCATGY | 3 cut(s) | 743, 770, 784 |
| BstSCI | CCNGG | 3 cut(s) | 536, 937, 1000 |
| BstV1I | GCAGC | 5 cut(s) | 87, 91, 206, 940, 1036 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 235 |
| BsuI | GTATCC | 2 cut(s) | 531, 819 |
| BsuRI | GGCC | 2 cut(s) | 322, 657 |
| BtgI | CCRYGG | 1 cut(s) | 499 |
| BtsCI | GGATG | 3 cut(s) | 182, 811, 982 |
| BtsI | GCAGTG | 2 cut(s) | 850, 948 |
| BtsIMutI | CAGTG | 4 cut(s) | 282, 753, 850, 948 |
| BveI | ACCTGC | 1 cut(s) | 974 |
| CciI | TCATGA | 1 cut(s) | 832 |
| CfoI | GCGC | 2 cut(s) | 226, 404 |
| Cfr13I | GGNCC | 4 cut(s) | 320, 496, 532, 942 |
| Csp6I | GTAC | 2 cut(s) | 364, 610 |
| CviQI | GTAC | 2 cut(s) | 364, 610 |
| DdeI | CTNAG | 4 cut(s) | 18, 198, 248, 671 |
| DinI | GGCGCC | 2 cut(s) | 225, 403 |
| DpnI | GATC | 3 cut(s) | 240, 309, 606 |
| DpnII | GATC | 3 cut(s) | 238, 307, 604 |
| DraIII | CACNNNGTG | 1 cut(s) | 271 |
| Eam1104I | CTCTTC | 1 cut(s) | 368 |
| EarI | CTCTTC | 1 cut(s) | 368 |
| Eco130I | CCWWGG | 3 cut(s) | 499, 658, 1038 |
| Eco47I | GGWCC | 3 cut(s) | 496, 532, 942 |
| Eco57I | CTGAAG | 1 cut(s) | 49 |
| Eco88I | CYCGRG | 1 cut(s) | 218 |
| EcoRI | GAATTC | 1 cut(s) | 1028 |
| EcoRII | CCWGG | 2 cut(s) | 536, 937 |
| EcoT14I | CCWWGG | 3 cut(s) | 499, 658, 1038 |
| EgeI | GGCGCC | 2 cut(s) | 225, 403 |
| EheI | GGCGCC | 2 cut(s) | 225, 403 |
| ErhI | CCWWGG | 3 cut(s) | 499, 658, 1038 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 419, 451, 909, 941 |
| FaqI | GGGAC | 1 cut(s) | 955 |
| FauI | CCCGC | 2 cut(s) | 157, 163 |
| FbaI | TGATCA | 1 cut(s) | 307 |
| Fnu4HI | GCNGC | 5 cut(s) | 80, 101, 195, 954, 1050 |
| FokI | GGATG | 3 cut(s) | 169, 818, 989 |
| Fsp4HI | GCNGC | 5 cut(s) | 80, 101, 195, 954, 1050 |
| FspBI | CTAG | 2 cut(s) | 83, 203 |
| GlaI | GCGC | 2 cut(s) | 225, 403 |
| GluI | GCNGC | 5 cut(s) | 80, 101, 195, 954, 1050 |
| GsuI | CTGGAG | 1 cut(s) | 777 |
| HaeII | RGCGCY | 2 cut(s) | 227, 405 |
| HaeIII | GGCC | 2 cut(s) | 322, 657 |
| HapII | CCGG | 2 cut(s) | 451, 1001 |
| HhaI | GCGC | 2 cut(s) | 226, 404 |
| Hin1I | GRCGYC | 3 cut(s) | 32, 224, 402 |
| Hin6I | GCGC | 2 cut(s) | 224, 402 |
| HinP1I | GCGC | 2 cut(s) | 224, 402 |
| HincII | GTYRAC | 1 cut(s) | 316 |
| HindII | GTYRAC | 1 cut(s) | 316 |
| HindIII | AAGCTT | 1 cut(s) | 61 |
| HinfI | GANTC | 3 cut(s) | 418, 480, 667 |
| HpaII | CCGG | 2 cut(s) | 451, 1001 |
| Hpy166II | GTNNAC | 2 cut(s) | 36, 316 |
| Hpy188I | TCNGA | 2 cut(s) | 333, 532 |
| Hpy188III | TCNNGA | 5 cut(s) | 422, 592, 602, 833, 865 |
| Hpy8I | GTNNAC | 2 cut(s) | 36, 316 |
| HpyAV | CCTTC | 2 cut(s) | 467, 756 |
| HpyCH4III | ACNGT | 2 cut(s) | 14, 286 |
| HpyCH4IV | ACGT | 1 cut(s) | 32 |
| HpyCH4V | TGCA | 7 cut(s) | 79, 115, 194, 282, 570, 788, 956 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 410, 631, 1049 |
| HpyF3I | CTNAG | 4 cut(s) | 18, 198, 248, 671 |
| HpySE526I | ACGT | 1 cut(s) | 32 |
| Hsp92I | GRCGYC | 3 cut(s) | 32, 224, 402 |
| HspAI | GCGC | 2 cut(s) | 224, 402 |
| KasI | GGCGCC | 2 cut(s) | 223, 401 |
| Ksp22I | TGATCA | 1 cut(s) | 307 |
| Kzo9I | GATC | 3 cut(s) | 238, 307, 604 |
| LmnI | GCTCC | 2 cut(s) | 355, 867 |
| Lsp1109I | GCAGC | 5 cut(s) | 87, 91, 206, 940, 1036 |
| LweI | GCATC | 2 cut(s) | 717, 796 |
| MaeI | CTAG | 2 cut(s) | 83, 203 |
| MaeII | ACGT | 1 cut(s) | 32 |
| MaeIII | GTNAC | 4 cut(s) | 85, 263, 664, 875 |
| MalI | GATC | 3 cut(s) | 240, 309, 606 |
| MboI | GATC | 3 cut(s) | 238, 307, 604 |
| MboII | GAAGA | 8 cut(s) | 56, 59, 62, 65, 68, 385, 395, 917 |
| MfeI | CAATTG | 1 cut(s) | 277 |
| MhlI | GDGCHC | 5 cut(s) | 8, 276, 627, 852, 965 |
| MluCI | AATT | 7 cut(s) | 277, 325, 597, 871, 906, 1028, 1090 |
| Mly113I | GGCGCC | 2 cut(s) | 224, 402 |
| MlyI | GAGTC | 2 cut(s) | 412, 661 |
| MmeI | TCCRAC | 2 cut(s) | 216, 751 |
| MnlI | CCTC | 8 cut(s) | 190, 214, 327, 470, 705, 735, 767, 998 |
| MroXI | GAANNNNTTC | 1 cut(s) | 921 |
| MseI | TTAA | 6 cut(s) | 291, 489, 543, 630, 702, 1113 |
| MslI | CAYNNNNRTG | 3 cut(s) | 563, 758, 815 |
| MspA1I | CMGCKG | 1 cut(s) | 444 |
| MspCI | CTTAAG | 1 cut(s) | 629 |
| MspI | CCGG | 2 cut(s) | 451, 1001 |
| MspR9I | CCNGG | 3 cut(s) | 538, 939, 1002 |
| MunI | CAATTG | 1 cut(s) | 277 |
| MvaI | CCWGG | 2 cut(s) | 538, 939 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 410, 631, 1049 |
| NarI | GGCGCC | 2 cut(s) | 224, 402 |
| NciI | CCSGG | 1 cut(s) | 1002 |
| NcoI | CCATGG | 1 cut(s) | 499 |
| NdeII | GATC | 3 cut(s) | 238, 307, 604 |
| NlaIV | GGNNCC | 7 cut(s) | 208, 225, 403, 449, 497, 534, 943 |
| NmuCI | GTSAC | 3 cut(s) | 85, 263, 664 |
| NspI | RCATGY | 3 cut(s) | 743, 770, 784 |
| OliI | CACNNNNGTG | 1 cut(s) | 758 |
| PagI | TCATGA | 1 cut(s) | 832 |
| PaqCI | CACCTGC | 1 cut(s) | 974 |
| PciI | ACATGT | 2 cut(s) | 766, 780 |
| PdmI | GAANNNNTTC | 1 cut(s) | 921 |
| PfeI | GAWTC | 1 cut(s) | 480 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 896 |
| PkrI | GCNGC | 5 cut(s) | 81, 102, 196, 955, 1051 |
| PleI | GAGTC | 2 cut(s) | 412, 661 |
| PluTI | GGCGCC | 2 cut(s) | 227, 405 |
| PpsI | GAGTC | 2 cut(s) | 412, 661 |
| PscI | ACATGT | 2 cut(s) | 766, 780 |
| Psp6I | CCWGG | 2 cut(s) | 536, 937 |
| PspGI | CCWGG | 2 cut(s) | 536, 937 |
| PspN4I | GGNNCC | 7 cut(s) | 208, 225, 403, 449, 497, 534, 943 |
| PspPI | GGNCC | 4 cut(s) | 320, 496, 532, 942 |
| PvuII | CAGCTG | 1 cut(s) | 444 |
| RsaI | GTAC | 2 cut(s) | 365, 611 |
| RsaNI | GTAC | 2 cut(s) | 364, 610 |
| RseI | CAYNNNNRTG | 3 cut(s) | 563, 758, 815 |
| SaqAI | TTAA | 6 cut(s) | 291, 489, 543, 630, 702, 1113 |
| SatI | GCNGC | 5 cut(s) | 80, 101, 195, 954, 1050 |
| Sau3AI | GATC | 3 cut(s) | 238, 307, 604 |
| Sau96I | GGNCC | 4 cut(s) | 320, 496, 532, 942 |
| ScaI | AGTACT | 1 cut(s) | 365 |
| SchI | GAGTC | 2 cut(s) | 412, 661 |
| ScrFI | CCNGG | 3 cut(s) | 538, 939, 1002 |
| SduI | GDGCHC | 5 cut(s) | 8, 276, 627, 852, 965 |
| SfaNI | GCATC | 2 cut(s) | 717, 796 |
| SfoI | GGCGCC | 2 cut(s) | 225, 403 |
| SinI | GGWCC | 3 cut(s) | 496, 532, 942 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 563, 758, 815 |
| SmlI | CTYRAG | 2 cut(s) | 420, 629 |
| SmoI | CTYRAG | 2 cut(s) | 420, 629 |
| Sse9I | AATT | 7 cut(s) | 277, 325, 597, 871, 906, 1028, 1090 |
| SsiI | CCGC | 2 cut(s) | 164, 170 |
| SspDI | GGCGCC | 2 cut(s) | 223, 401 |
| SspI | AATATT | 1 cut(s) | 1079 |
| SspMI | CTAG | 2 cut(s) | 83, 203 |
| StyD4I | CCNGG | 3 cut(s) | 536, 937, 1000 |
| StyI | CCWWGG | 3 cut(s) | 499, 658, 1038 |
| TaaI | ACNGT | 2 cut(s) | 14, 286 |
| TaiI | ACGT | 1 cut(s) | 35 |
| TaqI | TCGA | 2 cut(s) | 141, 241 |
| TasI | AATT | 7 cut(s) | 277, 325, 597, 871, 906, 1028, 1090 |
| TatI | WGTACW | 2 cut(s) | 363, 609 |
| TfiI | GAWTC | 1 cut(s) | 480 |
| Tru1I | TTAA | 6 cut(s) | 291, 489, 543, 630, 702, 1113 |
| Tru9I | TTAA | 6 cut(s) | 291, 489, 543, 630, 702, 1113 |
| TscAI | CASTG | 4 cut(s) | 289, 760, 857, 955 |
| TseFI | GTSAC | 3 cut(s) | 85, 263, 664 |
| TseI | GCWGC | 5 cut(s) | 79, 100, 194, 953, 1049 |
| Tsp45I | GTSAC | 3 cut(s) | 85, 263, 664 |
| TspDTI | ATGAA | 5 cut(s) | 396, 821, 833, 1021, 1089 |
| TspRI | CASTG | 4 cut(s) | 289, 760, 857, 955 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 896 |
| Vha464I | CTTAAG | 1 cut(s) | 629 |
| VpaK11BI | GGWCC | 3 cut(s) | 496, 532, 942 |
| XapI | RAATTY | 2 cut(s) | 597, 1028 |
| XceI | RCATGY | 3 cut(s) | 743, 770, 784 |
| XmnI | GAANNNNTTC | 1 cut(s) | 921 |
| XspI | CTAG | 2 cut(s) | 83, 203 |
| ZraI | GACGTC | 1 cut(s) | 33 |
| ZrmI | AGTACT | 1 cut(s) | 365 |