Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 3 cut(s) | 131, 326, 420 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 821 |
| AciI | CCGC | 2 cut(s) | 89, 95 |
| AclWI | GGATC | 3 cut(s) | 163, 171, 176 |
| AcsI | RAATTY | 2 cut(s) | 522, 953 |
| AcyI | GRCGYC | 1 cut(s) | 327 |
| AdeI | CACNNNGTG | 1 cut(s) | 196 |
| AfaI | GTAC | 1 cut(s) | 290 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 821 |
| AflII | CTTAAG | 1 cut(s) | 554 |
| AflIII | ACRYGT | 2 cut(s) | 691, 705 |
| AgsI | TTSAA | 6 cut(s) | 347, 382, 481, 496, 723, 784 |
| AjnI | CCWGG | 3 cut(s) | 448, 461, 925 |
| AleI | CACNNNNGTG | 2 cut(s) | 683, 878 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 356, 388 |
| AluBI | AGCT | 6 cut(s) | 7, 55, 122, 369, 787, 974 |
| AluI | AGCT | 6 cut(s) | 7, 55, 122, 369, 787, 974 |
| Alw21I | GWGCWC | 3 cut(s) | 201, 552, 890 |
| Alw26I | GTCTC | 3 cut(s) | 104, 696, 860 |
| AlwI | GGATC | 3 cut(s) | 163, 171, 176 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 977 |
| AoxI | GGCC | 2 cut(s) | 245, 580 |
| ApeKI | GCWGC | 3 cut(s) | 4, 119, 974 |
| ApoI | RAATTY | 2 cut(s) | 522, 953 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 846 |
| AspLEI | GCGC | 3 cut(s) | 25, 151, 329 |
| AspS9I | GGNCC | 2 cut(s) | 245, 457 |
| AsuHPI | GGTGA | 1 cut(s) | 22 |
| AvaII | GGWCC | 1 cut(s) | 457 |
| AxyI | CCTNAGG | 1 cut(s) | 173 |
| BamHI | GGATCC | 1 cut(s) | 168 |
| BanI | GGYRCC | 3 cut(s) | 131, 326, 420 |
| BanII | GRGCYC | 1 cut(s) | 926 |
| BauI | CACGAG | 1 cut(s) | 651 |
| BbsI | GAAGAC | 1 cut(s) | 349 |
| Bbv12I | GWGCWC | 3 cut(s) | 201, 552, 890 |
| BbvI | GCAGC | 3 cut(s) | 16, 131, 961 |
| BccI | CCATC | 2 cut(s) | 236, 328 |
| BciT130I | CCWGG | 3 cut(s) | 450, 463, 927 |
| BciVI | GTATCC | 2 cut(s) | 456, 744 |
| BclI | TGATCA | 1 cut(s) | 232 |
| BcoDI | GTCTC | 3 cut(s) | 104, 696, 860 |
| BfaI | CTAG | 2 cut(s) | 612, 870 |
| BfoI | RGCGCY | 1 cut(s) | 330 |
| BfrI | CTTAAG | 1 cut(s) | 554 |
| BfuI | GTATCC | 2 cut(s) | 456, 744 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 335 |
| BisI | GCNGC | 3 cut(s) | 5, 120, 975 |
| BlsI | GCNGC | 3 cut(s) | 6, 121, 976 |
| BmcAI | AGTACT | 1 cut(s) | 290 |
| Bme1390I | CCNGG | 3 cut(s) | 450, 463, 927 |
| Bme18I | GGWCC | 1 cut(s) | 457 |
| BmgT120I | GGNCC | 2 cut(s) | 245, 457 |
| BmiI | GGNNCC | 6 cut(s) | 133, 170, 328, 374, 422, 459 |
| BmrFI | CCNGG | 3 cut(s) | 450, 463, 927 |
| BmsI | GCATC | 4 cut(s) | 136, 559, 655, 721 |
| BpiI | GAAGAC | 1 cut(s) | 349 |
| BpmI | CTGGAG | 1 cut(s) | 784 |
| BsaBI | GATNNNNATC | 1 cut(s) | 231 |
| BsaHI | GRCGYC | 1 cut(s) | 327 |
| BsaI | GGTCTC | 1 cut(s) | 104 |
| BsaJI | CCNNGG | 5 cut(s) | 424, 461, 583, 925, 963 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 821 |
| Bse1I | ACTGG | 4 cut(s) | 186, 376, 767, 854 |
| Bse21I | CCTNAGG | 1 cut(s) | 173 |
| Bse3DI | GCAATG | 1 cut(s) | 495 |
| Bse8I | GATNNNNATC | 1 cut(s) | 231 |
| BseBI | CCWGG | 3 cut(s) | 450, 463, 927 |
| BseDI | CCNNGG | 5 cut(s) | 424, 461, 583, 925, 963 |
| BseGI | GGATG | 1 cut(s) | 736 |
| BseJI | GATNNNNATC | 1 cut(s) | 231 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 821 |
| BseMI | GCAATG | 1 cut(s) | 495 |
| BseMII | CTCAG | 2 cut(s) | 137, 187 |
| BseNI | ACTGG | 4 cut(s) | 186, 376, 767, 854 |
| BseRI | GAGGAG | 2 cut(s) | 94, 714 |
| BseXI | GCAGC | 3 cut(s) | 16, 131, 961 |
| BseYI | CCCAGC | 2 cut(s) | 572, 977 |
| BshFI | GGCC | 2 cut(s) | 247, 582 |
| BshNI | GGYRCC | 3 cut(s) | 131, 326, 420 |
| BsiHKAI | GWGCWC | 3 cut(s) | 201, 552, 890 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 821 |
| BsmAI | GTCTC | 3 cut(s) | 104, 696, 860 |
| BsnI | GGCC | 2 cut(s) | 247, 582 |
| Bso31I | GGTCTC | 1 cut(s) | 104 |
| Bsp1286I | GDGCHC | 4 cut(s) | 201, 552, 890, 926 |
| Bsp143I | GATC | 4 cut(s) | 163, 168, 232, 529 |
| BspACI | CCGC | 2 cut(s) | 89, 95 |
| BspANI | GGCC | 2 cut(s) | 247, 582 |
| BspCNI | CTCAG | 2 cut(s) | 136, 186 |
| BspHI | TCATGA | 1 cut(s) | 757 |
| BspLI | GGNNCC | 6 cut(s) | 133, 170, 328, 374, 422, 459 |
| BspPI | GGATC | 3 cut(s) | 163, 171, 176 |
| BspQI | GCTCTTC | 1 cut(s) | 878 |
| BspT107I | GGYRCC | 3 cut(s) | 131, 326, 420 |
| BspTI | CTTAAG | 1 cut(s) | 554 |
| BspTNI | GGTCTC | 1 cut(s) | 104 |
| BsrDI | GCAATG | 1 cut(s) | 495 |
| BsrI | ACTGG | 4 cut(s) | 186, 376, 767, 854 |
| BssECI | CCNNGG | 5 cut(s) | 424, 461, 583, 925, 963 |
| BssMI | GATC | 4 cut(s) | 163, 168, 232, 529 |
| BssNI | GRCGYC | 1 cut(s) | 327 |
| BssSI | CACGAG | 1 cut(s) | 651 |
| BssT1I | CCWWGG | 2 cut(s) | 583, 963 |
| Bst2BI | CACGAG | 1 cut(s) | 651 |
| Bst2UI | CCWGG | 3 cut(s) | 450, 463, 927 |
| Bst4CI | ACNGT | 2 cut(s) | 211, 425 |
| Bst6I | CTCTTC | 2 cut(s) | 293, 878 |
| BstACI | GRCGYC | 1 cut(s) | 327 |
| BstAFI | CTTAAG | 1 cut(s) | 554 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 974 |
| BstC8I | GCNNGC | 2 cut(s) | 431, 435 |
| BstDEI | CTNAG | 3 cut(s) | 123, 173, 596 |
| BstDSI | CCRYGG | 1 cut(s) | 424 |
| BstF5I | GGATG | 1 cut(s) | 736 |
| BstH2I | RGCGCY | 1 cut(s) | 330 |
| BstHHI | GCGC | 3 cut(s) | 25, 151, 329 |
| BstKTI | GATC | 4 cut(s) | 166, 171, 235, 532 |
| BstMAI | GTCTC | 3 cut(s) | 104, 696, 860 |
| BstMBI | GATC | 4 cut(s) | 163, 168, 232, 529 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 335, 556, 974 |
| BstNI | CCWGG | 3 cut(s) | 450, 463, 927 |
| BstNSI | RCATGY | 3 cut(s) | 437, 695, 709 |
| BstSCI | CCNGG | 3 cut(s) | 448, 461, 925 |
| BstV1I | GCAGC | 3 cut(s) | 16, 131, 961 |
| BstV2I | GAAGAC | 1 cut(s) | 349 |
| BstX2I | RGATCY | 1 cut(s) | 168 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 160 |
| BstYI | RGATCY | 1 cut(s) | 168 |
| Bsu36I | CCTNAGG | 1 cut(s) | 173 |
| BsuI | GTATCC | 2 cut(s) | 456, 744 |
| BsuRI | GGCC | 2 cut(s) | 247, 582 |
| BtgI | CCRYGG | 1 cut(s) | 424 |
| BtsCI | GGATG | 1 cut(s) | 736 |
| BtsI | GCAGTG | 3 cut(s) | 18, 775, 873 |
| BtsIMutI | CAGTG | 4 cut(s) | 18, 207, 775, 873 |
| Cac8I | GCNNGC | 2 cut(s) | 431, 435 |
| CaiI | CAGNNNCTG | 1 cut(s) | 977 |
| CciI | TCATGA | 1 cut(s) | 757 |
| CfoI | GCGC | 3 cut(s) | 25, 151, 329 |
| Cfr13I | GGNCC | 2 cut(s) | 245, 457 |
| Csp6I | GTAC | 1 cut(s) | 289 |
| CviAII | CATG | 6 cut(s) | 154, 331, 434, 692, 706, 758 |
| CviQI | GTAC | 1 cut(s) | 289 |
| DdeI | CTNAG | 3 cut(s) | 123, 173, 596 |
| DinI | GGCGCC | 1 cut(s) | 328 |
| DpnI | GATC | 4 cut(s) | 165, 170, 234, 531 |
| DpnII | GATC | 4 cut(s) | 163, 168, 232, 529 |
| DraIII | CACNNNGTG | 1 cut(s) | 196 |
| Eam1104I | CTCTTC | 2 cut(s) | 293, 878 |
| EarI | CTCTTC | 2 cut(s) | 293, 878 |
| Eco130I | CCWWGG | 2 cut(s) | 583, 963 |
| Eco24I | GRGCYC | 1 cut(s) | 926 |
| Eco31I | GGTCTC | 1 cut(s) | 104 |
| Eco47I | GGWCC | 1 cut(s) | 457 |
| Eco81I | CCTNAGG | 1 cut(s) | 173 |
| EcoRI | GAATTC | 1 cut(s) | 953 |
| EcoRII | CCWGG | 3 cut(s) | 448, 461, 925 |
| EcoT14I | CCWWGG | 2 cut(s) | 583, 963 |
| EcoT38I | GRGCYC | 1 cut(s) | 926 |
| EgeI | GGCGCC | 1 cut(s) | 328 |
| EheI | GGCGCC | 1 cut(s) | 328 |
| ErhI | CCWWGG | 2 cut(s) | 583, 963 |
| FaeI | CATG | 6 cut(s) | 157, 334, 437, 695, 709, 761 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 344, 376, 834, 866 |
| FatI | CATG | 6 cut(s) | 153, 330, 433, 691, 705, 757 |
| FauI | CCCGC | 2 cut(s) | 82, 88 |
| FbaI | TGATCA | 1 cut(s) | 232 |
| Fnu4HI | GCNGC | 3 cut(s) | 5, 120, 975 |
| FokI | GGATG | 1 cut(s) | 743 |
| FriOI | GRGCYC | 1 cut(s) | 926 |
| Fsp4HI | GCNGC | 3 cut(s) | 5, 120, 975 |
| FspBI | CTAG | 2 cut(s) | 612, 870 |
| GlaI | GCGC | 3 cut(s) | 24, 150, 328 |
| GluI | GCNGC | 3 cut(s) | 5, 120, 975 |
| GsaI | CCCAGC | 2 cut(s) | 576, 981 |
| GsuI | CTGGAG | 1 cut(s) | 784 |
| HaeII | RGCGCY | 1 cut(s) | 330 |
| HaeIII | GGCC | 2 cut(s) | 247, 582 |
| HhaI | GCGC | 3 cut(s) | 25, 151, 329 |
| Hin1I | GRCGYC | 1 cut(s) | 327 |
| Hin1II | CATG | 6 cut(s) | 157, 334, 437, 695, 709, 761 |
| Hin6I | GCGC | 3 cut(s) | 23, 149, 327 |
| HinP1I | GCGC | 3 cut(s) | 23, 149, 327 |
| HinfI | GANTC | 3 cut(s) | 343, 405, 592 |
| HphI | GGTGA | 1 cut(s) | 22 |
| Hpy166II | GTNNAC | 1 cut(s) | 36 |
| Hpy188I | TCNGA | 4 cut(s) | 126, 168, 258, 995 |
| Hpy188III | TCNNGA | 6 cut(s) | 347, 517, 527, 612, 758, 790 |
| Hpy8I | GTNNAC | 1 cut(s) | 36 |
| HpyAV | CCTTC | 2 cut(s) | 392, 681 |
| HpyCH4III | ACNGT | 2 cut(s) | 211, 425 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 207, 713 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 335, 556, 974 |
| HpyF3I | CTNAG | 3 cut(s) | 123, 173, 596 |
| Hsp92I | GRCGYC | 1 cut(s) | 327 |
| Hsp92II | CATG | 6 cut(s) | 157, 334, 437, 695, 709, 761 |
| HspAI | GCGC | 3 cut(s) | 23, 149, 327 |
| KasI | GGCGCC | 1 cut(s) | 326 |
| Ksp22I | TGATCA | 1 cut(s) | 232 |
| Kzo9I | GATC | 4 cut(s) | 163, 168, 232, 529 |
| LguI | GCTCTTC | 1 cut(s) | 878 |
| LmnI | GCTCC | 1 cut(s) | 792 |
| Lsp1109I | GCAGC | 3 cut(s) | 16, 131, 961 |
| LweI | GCATC | 4 cut(s) | 136, 559, 655, 721 |
| MaeI | CTAG | 2 cut(s) | 612, 870 |
| MaeIII | GTNAC | 5 cut(s) | 10, 188, 589, 800, 1013 |
| MalI | GATC | 4 cut(s) | 165, 170, 234, 531 |
| MboI | GATC | 4 cut(s) | 163, 168, 232, 529 |
| MboII | GAAGA | 4 cut(s) | 310, 349, 842, 895 |
| MfeI | CAATTG | 1 cut(s) | 202 |
| MflI | RGATCY | 1 cut(s) | 168 |
| MhlI | GDGCHC | 4 cut(s) | 201, 552, 890, 926 |
| MluCI | AATT | 8 cut(s) | 41, 202, 250, 522, 796, 831, 897, 953 |
| Mly113I | GGCGCC | 1 cut(s) | 327 |
| MlyI | GAGTC | 1 cut(s) | 586 |
| MmeI | TCCRAC | 2 cut(s) | 141, 676 |
| MnlI | CCTC | 9 cut(s) | 115, 182, 252, 395, 630, 660, 692, 901, 923 |
| MroXI | GAANNNNTTC | 1 cut(s) | 846 |
| MseI | TTAA | 7 cut(s) | 216, 414, 468, 555, 627, 636, 1038 |
| MslI | CAYNNNNRTG | 5 cut(s) | 240, 488, 683, 740, 878 |
| MspA1I | CMGCKG | 3 cut(s) | 7, 369, 974 |
| MspCI | CTTAAG | 1 cut(s) | 554 |
| MspR9I | CCNGG | 3 cut(s) | 450, 463, 927 |
| MunI | CAATTG | 1 cut(s) | 202 |
| MvaI | CCWGG | 3 cut(s) | 450, 463, 927 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 335, 556, 974 |
| NarI | GGCGCC | 1 cut(s) | 327 |
| NdeII | GATC | 4 cut(s) | 163, 168, 232, 529 |
| NlaIII | CATG | 6 cut(s) | 157, 334, 437, 695, 709, 761 |
| NlaIV | GGNNCC | 6 cut(s) | 133, 170, 328, 374, 422, 459 |
| NmuCI | GTSAC | 3 cut(s) | 10, 188, 589 |
| NspI | RCATGY | 3 cut(s) | 437, 695, 709 |
| OliI | CACNNNNGTG | 2 cut(s) | 683, 878 |
| PaeI | GCATGC | 1 cut(s) | 437 |
| PagI | TCATGA | 1 cut(s) | 757 |
| PciI | ACATGT | 2 cut(s) | 691, 705 |
| PciSI | GCTCTTC | 1 cut(s) | 878 |
| PdmI | GAANNNNTTC | 1 cut(s) | 846 |
| PfeI | GAWTC | 2 cut(s) | 343, 405 |
| PflFI | GACNNNGTC | 1 cut(s) | 353 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 821 |
| PkrI | GCNGC | 3 cut(s) | 6, 121, 976 |
| PleI | GAGTC | 1 cut(s) | 586 |
| PluTI | GGCGCC | 1 cut(s) | 330 |
| PpsI | GAGTC | 1 cut(s) | 586 |
| PscI | ACATGT | 2 cut(s) | 691, 705 |
| Psp6I | CCWGG | 3 cut(s) | 448, 461, 925 |
| PspFI | CCCAGC | 2 cut(s) | 572, 977 |
| PspGI | CCWGG | 3 cut(s) | 448, 461, 925 |
| PspN4I | GGNNCC | 6 cut(s) | 133, 170, 328, 374, 422, 459 |
| PspPI | GGNCC | 2 cut(s) | 245, 457 |
| PstNI | CAGNNNCTG | 1 cut(s) | 977 |
| PsuI | RGATCY | 1 cut(s) | 168 |
| PsyI | GACNNNGTC | 1 cut(s) | 353 |
| PvuII | CAGCTG | 3 cut(s) | 7, 369, 974 |
| RsaI | GTAC | 1 cut(s) | 290 |
| RsaNI | GTAC | 1 cut(s) | 289 |
| RseI | CAYNNNNRTG | 5 cut(s) | 240, 488, 683, 740, 878 |
| SapI | GCTCTTC | 1 cut(s) | 878 |
| SaqAI | TTAA | 7 cut(s) | 216, 414, 468, 555, 627, 636, 1038 |
| SatI | GCNGC | 3 cut(s) | 5, 120, 975 |
| Sau3AI | GATC | 4 cut(s) | 163, 168, 232, 529 |
| Sau96I | GGNCC | 2 cut(s) | 245, 457 |
| ScaI | AGTACT | 1 cut(s) | 290 |
| SchI | GAGTC | 1 cut(s) | 586 |
| ScrFI | CCNGG | 3 cut(s) | 450, 463, 927 |
| SduI | GDGCHC | 4 cut(s) | 201, 552, 890, 926 |
| SfaNI | GCATC | 4 cut(s) | 136, 559, 655, 721 |
| SfoI | GGCGCC | 1 cut(s) | 328 |
| SinI | GGWCC | 1 cut(s) | 457 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 240, 488, 683, 740, 878 |
| SmlI | CTYRAG | 1 cut(s) | 554 |
| SmoI | CTYRAG | 1 cut(s) | 554 |
| SphI | GCATGC | 1 cut(s) | 437 |
| Sse9I | AATT | 8 cut(s) | 41, 202, 250, 522, 796, 831, 897, 953 |
| SsiI | CCGC | 2 cut(s) | 89, 95 |
| SspDI | GGCGCC | 1 cut(s) | 326 |
| SspMI | CTAG | 2 cut(s) | 612, 870 |
| StyD4I | CCNGG | 3 cut(s) | 448, 461, 925 |
| StyI | CCWWGG | 2 cut(s) | 583, 963 |
| TaaI | ACNGT | 2 cut(s) | 211, 425 |
| TaqI | TCGA | 1 cut(s) | 66 |
| TasI | AATT | 8 cut(s) | 41, 202, 250, 522, 796, 831, 897, 953 |
| TatI | WGTACW | 1 cut(s) | 288 |
| TfiI | GAWTC | 2 cut(s) | 343, 405 |
| Tru1I | TTAA | 7 cut(s) | 216, 414, 468, 555, 627, 636, 1038 |
| Tru9I | TTAA | 7 cut(s) | 216, 414, 468, 555, 627, 636, 1038 |
| TscAI | CASTG | 4 cut(s) | 25, 214, 782, 880 |
| TseFI | GTSAC | 3 cut(s) | 10, 188, 589 |
| TseI | GCWGC | 3 cut(s) | 4, 119, 974 |
| Tsp45I | GTSAC | 3 cut(s) | 10, 188, 589 |
| TspDTI | ATGAA | 6 cut(s) | 321, 746, 758, 786, 946, 1014 |
| TspRI | CASTG | 4 cut(s) | 25, 214, 782, 880 |
| Tth111I | GACNNNGTC | 1 cut(s) | 353 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 821 |
| Vha464I | CTTAAG | 1 cut(s) | 554 |
| VpaK11BI | GGWCC | 1 cut(s) | 457 |
| XapI | RAATTY | 2 cut(s) | 522, 953 |
| XbaI | TCTAGA | 1 cut(s) | 611 |
| XceI | RCATGY | 3 cut(s) | 437, 695, 709 |
| XmnI | GAANNNNTTC | 1 cut(s) | 846 |
| XspI | CTAG | 2 cut(s) | 612, 870 |
| ZrmI | AGTACT | 1 cut(s) | 290 |