MD10G1030200.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000357
Physical Location & Seq
Forward (+)
3969816 .. 3971573
1758 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1030200.v1.1.491

Sequence Viewer

Length: 612 bp
ATGCTTTCTCTTCCAAATGATATGTCCCAACTGAAAGATGCAGTTGTTGAAGGAGGAATTCCATTTAACAGGGTCCATGGAAAGCATTCTTTTGAGTACCTAGGTTTAGACCCCAGGTTTAATCAAGTTTTCAACACAACAATTTTTAACCACACCAATATTGTCACCAAGAAGATTCTTCATATCTACAAGGGTTTTGAGAAAATTACCCTACTTGTTGATGTTGGTGGTGGTTTGGGAGTCACTATTAGGGTGGAACATGTTGGAGGAGACATGTTTGCAAGTGTTCCATCTGGGGATGCCATTTTTAAGAAGTTGATACTTCACGATTGGAGAGACCAGCACTGCCTAAAGCTGTTGGAAAATTGTTACAATGCTATACCAGACGATGGGAAAGTGATTATCGTGGACGCACTTATTCCAGTAATGCCAGAGACTAGCACCGCCGTGAAGAGCACCTCCCAAATTGATGTGCTTATGATGACTCAAAACCAGGGAGGAAAGGAGCAGAGCCGAGAAGAGTTCATGGCTTTGGCAACTGGTGCACGATTTAGTGGCATTAAATATGAATGTTTTGTCTGTAATTTTTGGGTGATGGAGATCTTTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01058 ko01100 ko01110 map00940 map01058 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

204

Amino Acids

22.74

Weight (kDa)

6.15

Isoelectric Point (pI)

22.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 9 - 83 1.1e-14 O-methyltransferase domain
Methyltransf_2 PF00891 84 - 185 4e-28 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000357)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17810 FvH4_7g17811 FvH4_7g17820 FvH4_7g17830
malus_domestica MD00G1100700.v1.1 MD01G1089600.v1.1 MD01G1089800.v1.1 MD01G1090400.v1.1 MD01G1090500.v1.1 MD01G1090800.v1.1 MD04G1141100.v1.1 MD07G1161000.v1.1 MD07G1161100.v1.1 MD10G1030000.v1.1 MD10G1030200.v1.1 MD15G1409200.v1.1 MD15G1409700.v1.1
prunus_persica Prupe.2G199100_v2.0.a1 Prupe.2G199100_v2.0.a1 Prupe.2G199300_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199500_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199800_v2.0.a1 Prupe.2G200100_v2.0.a1 Prupe.2G200100_v2.0.a1
pyrus_communis pycom01g11360 pycom01g11370 pycom01g11400 pycom01g11440 pycom01g11480 pycom01g11490 pycom07g15690 pycom08g12100
rosa_chinensis RchiOBHm_Chr1g0317931 RchiOBHm_Chr1g0361211 RchiOBHm_Chr1g0361221 RchiOBHm_Chr1g0361241 RchiOBHm_Chr1g0367691 RchiOBHm_Chr4g0390171 RchiOBHm_Chr4g0396271 RchiOBHm_Chr6g0286421
rosa_laevigata RLG00000002555 RLG00000009555 RLG00000027275 RLG00000027731 RLG00000027732 RLG00000027734 RLG00000027738 RLG00000030575
rosa_multiflora Rmu_co8470451.1_g000001 Rmu_sc0000250.1_g000010 Rmu_sc0010379.1_g000006 Rmu_sc0011132.1_g000005 Rmu_sc0011132.1_g000007 Rmu_sc0038028.1_g000001 Rmu_ssc0000008.1_g000043 Rmu_ssc0000234.1_g000002 Rmu_ssc0000418.1_g000001
rosa_roxburghii Rroxscaffold_2G00116370 Rroxscaffold_4G00289300 Rroxscaffold_4G00295130 Rroxscaffold_4G00295140 Rroxscaffold_4G00295160 Rroxscaffold_4G00295190 Rroxscaffold_4G00295220 Rroxscaffold_4G00330100 Rroxscaffold_5G00341100
rosa_rugosa Rorug01G0287600 Rorug01G0287700 Rorug01G0336200 Rorug01G0336200 Rorug03G0350700
rosa_samantha Rh1AG030300 Rh1AG298500 Rh1AG298600 Rh1AG298700 Rh1AG298900 Rh1BG262400 Rh1BG262500 Rh1BG262800 Rh1BG304800 Rh1CG029000 Rh1CG279800 Rh1CG279900 Rh1CG280000 Rh1CG280100 Rh1CG280200 Rh1CG320500 Rh1DG041200 Rh1DG292700 Rh1DG292800 Rh1DG337100 Rh3DG107500 Rh4AG128100 Rh4BG024900 Rh4CG072700 Rh4DG063000 Rh6CG295900 Rh6DG287700
rosa_wichuraiana Rw1G002290 Rw1G026410 Rw1G026420 Rw1G026440 Rw4G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 389
AciI CCGC 1 cut(s) 444
AcsI RAATTY 1 cut(s) 57
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 1 cut(s) 389
AflIII ACRYGT 2 cut(s) 259, 273
AgsI TTSAA 2 cut(s) 50, 133
AjnI CCWGG 2 cut(s) 113, 492
AleI CACNNNNGTG 1 cut(s) 446
AluBI AGCT 1 cut(s) 355
AluI AGCT 1 cut(s) 355
Alw21I GWGCWC 2 cut(s) 458, 547
Alw26I GTCTC 3 cut(s) 264, 330, 428
Alw44I GTGCAC 1 cut(s) 543
ApaLI GTGCAC 1 cut(s) 543
ApoI RAATTY 1 cut(s) 57
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
Asp700I GAANNNNTTC 1 cut(s) 85
AspA2I CCTAGG 1 cut(s) 100
AspS9I GGNCC 1 cut(s) 73
AsuHPI GGTGA 2 cut(s) 157, 604
AvaII GGWCC 1 cut(s) 73
AvrII CCTAGG 1 cut(s) 100
BaeGI GKGCMC 1 cut(s) 547
Bbv12I GWGCWC 2 cut(s) 458, 547
BccI CCATC 3 cut(s) 298, 383, 589
BceAI ACGGC 1 cut(s) 431
BciT130I CCWGG 2 cut(s) 115, 494
BcoDI GTCTC 3 cut(s) 264, 330, 428
BfaI CTAG 2 cut(s) 101, 438
BglII AGATCT 1 cut(s) 600
BlnI CCTAGG 1 cut(s) 100
Bme1390I CCNGG 2 cut(s) 115, 494
Bme18I GGWCC 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 73
BmiI GGNNCC 1 cut(s) 74
BmrFI CCNGG 2 cut(s) 115, 494
BmsI GCATC 2 cut(s) 28, 289
BsaBI GATNNNNATC 1 cut(s) 599
BsaI GGTCTC 1 cut(s) 330
BsaJI CCNNGG 4 cut(s) 76, 100, 113, 493
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 2 cut(s) 422, 544
Bse8I GATNNNNATC 1 cut(s) 599
BseBI CCWGG 2 cut(s) 115, 494
BseDI CCNNGG 4 cut(s) 76, 100, 113, 493
BseGI GGATG 1 cut(s) 304
BseJI GATNNNNATC 1 cut(s) 599
BseLI CCNNNNNNNGG 1 cut(s) 389
BseNI ACTGG 2 cut(s) 422, 544
BseRI GAGGAG 1 cut(s) 282
BseSI GKGCMC 1 cut(s) 547
BsiHKAI GWGCWC 2 cut(s) 458, 547
BslFI GGGAC 1 cut(s) 10
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 3 cut(s) 264, 330, 428
BsmFI GGGAC 1 cut(s) 10
BsmI GAATGC 1 cut(s) 85
Bso31I GGTCTC 1 cut(s) 330
Bsp1286I GDGCHC 2 cut(s) 458, 547
Bsp143I GATC 1 cut(s) 600
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 74
BspQI GCTCTTC 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 330
BsrI ACTGG 2 cut(s) 422, 544
BssECI CCNNGG 4 cut(s) 76, 100, 113, 493
BssMI GATC 1 cut(s) 600
BssT1I CCWWGG 2 cut(s) 76, 100
Bst2UI CCWGG 2 cut(s) 115, 494
Bst6I CTCTTC 3 cut(s) 15, 446, 513
BstAPI GCANNNNNTGC 1 cut(s) 542
BstDSI CCRYGG 1 cut(s) 76
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 1 cut(s) 603
BstMAI GTCTC 3 cut(s) 264, 330, 428
BstMBI GATC 1 cut(s) 600
BstMWI GCNNNNNNNGC 1 cut(s) 542
BstNI CCWGG 2 cut(s) 115, 494
BstNSI RCATGY 2 cut(s) 263, 277
BstSCI CCNGG 2 cut(s) 113, 492
BstSLI GKGCMC 1 cut(s) 547
BstX2I RGATCY 1 cut(s) 600
BstYI RGATCY 1 cut(s) 600
BtgI CCRYGG 1 cut(s) 76
BtsCI GGATG 1 cut(s) 304
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 73
CseI GACGC 1 cut(s) 419
Csp6I GTAC 1 cut(s) 97
CviAII CATG 4 cut(s) 77, 260, 274, 526
CviJI RGCY 3 cut(s) 355, 513, 530
CviKI_1 RGCY 3 cut(s) 355, 513, 530
CviQI GTAC 1 cut(s) 97
DpnI GATC 1 cut(s) 602
DpnII GATC 1 cut(s) 600
Eam1104I CTCTTC 3 cut(s) 15, 446, 513
EarI CTCTTC 3 cut(s) 15, 446, 513
Eco130I CCWWGG 2 cut(s) 76, 100
Eco31I GGTCTC 1 cut(s) 330
Eco47I GGWCC 1 cut(s) 73
EcoRI GAATTC 1 cut(s) 57
EcoRII CCWGG 2 cut(s) 113, 492
EcoT14I CCWWGG 2 cut(s) 76, 100
ErhI CCWWGG 2 cut(s) 76, 100
FaeI CATG 4 cut(s) 80, 263, 277, 529
FaiI YATR 9 cut(s) 23, 78, 183, 261, 275, 380, 479, 527, 567
FaqI GGGAC 1 cut(s) 10
FatI CATG 4 cut(s) 76, 259, 273, 525
FokI GGATG 1 cut(s) 311
FspBI CTAG 2 cut(s) 101, 438
HgaI GACGC 1 cut(s) 419
Hin1II CATG 4 cut(s) 80, 263, 277, 529
HinfI GANTC 3 cut(s) 175, 240, 484
HphI GGTGA 2 cut(s) 157, 604
Hpy166II GTNNAC 2 cut(s) 409, 545
Hpy188III TCNNGA 1 cut(s) 326
Hpy8I GTNNAC 2 cut(s) 409, 545
HpyAV CCTTC 1 cut(s) 44
HpyCH4V TGCA 3 cut(s) 41, 281, 545
HpyF10VI GCNNNNNNNGC 1 cut(s) 542
Hsp92II CATG 4 cut(s) 80, 263, 277, 529
Kzo9I GATC 1 cut(s) 600
LguI GCTCTTC 1 cut(s) 446
LmnI GCTCC 1 cut(s) 505
LweI GCATC 2 cut(s) 28, 289
MaeI CTAG 2 cut(s) 101, 438
MaeIII GTNAC 3 cut(s) 163, 241, 368
MalI GATC 1 cut(s) 602
MboI GATC 1 cut(s) 600
MboII GAAGA 4 cut(s) 170, 184, 463, 530
MflI RGATCY 1 cut(s) 600
MhlI GDGCHC 2 cut(s) 458, 547
MluCI AATT 6 cut(s) 57, 141, 204, 364, 465, 583
MlyI GAGTC 2 cut(s) 249, 478
MmeI TCCRAC 2 cut(s) 244, 339
MnlI CCTC 4 cut(s) 47, 260, 469, 491
MroXI GAANNNNTTC 1 cut(s) 85
MseI TTAA 6 cut(s) 66, 120, 147, 309, 561, 606
MslI CAYNNNNRTG 1 cut(s) 446
MspR9I CCNGG 2 cut(s) 115, 494
Mva1269I GAATGC 1 cut(s) 85
MvaI CCWGG 2 cut(s) 115, 494
MwoI GCNNNNNNNGC 1 cut(s) 542
NcoI CCATGG 1 cut(s) 76
NdeII GATC 1 cut(s) 600
NlaIII CATG 4 cut(s) 80, 263, 277, 529
NlaIV GGNNCC 1 cut(s) 74
NmeAIII GCCGAG 1 cut(s) 539
NmuCI GTSAC 2 cut(s) 163, 241
NspI RCATGY 2 cut(s) 263, 277
OliI CACNNNNGTG 1 cut(s) 446
PciI ACATGT 2 cut(s) 259, 273
PciSI GCTCTTC 1 cut(s) 446
PctI GAATGC 1 cut(s) 85
PdmI GAANNNNTTC 1 cut(s) 85
PfeI GAWTC 1 cut(s) 175
PflMI CCANNNNNTGG 1 cut(s) 389
PleI GAGTC 2 cut(s) 248, 478
PpsI GAGTC 2 cut(s) 248, 478
PscI ACATGT 2 cut(s) 259, 273
Psp6I CCWGG 2 cut(s) 113, 492
PspGI CCWGG 2 cut(s) 113, 492
PspN4I GGNNCC 1 cut(s) 74
PspPI GGNCC 1 cut(s) 73
PsuI RGATCY 1 cut(s) 600
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
RseI CAYNNNNRTG 1 cut(s) 446
SapI GCTCTTC 1 cut(s) 446
SaqAI TTAA 6 cut(s) 66, 120, 147, 309, 561, 606
Sau3AI GATC 1 cut(s) 600
Sau96I GGNCC 1 cut(s) 73
SchI GAGTC 2 cut(s) 249, 478
ScrFI CCNGG 2 cut(s) 115, 494
SduI GDGCHC 2 cut(s) 458, 547
SetI ASST 5 cut(s) 102, 106, 119, 357, 461
SfaNI GCATC 2 cut(s) 28, 289
SinI GGWCC 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 446
Sse9I AATT 6 cut(s) 57, 141, 204, 364, 465, 583
SsiI CCGC 1 cut(s) 444
SspI AATATT 1 cut(s) 160
SspMI CTAG 2 cut(s) 101, 438
StyD4I CCNGG 2 cut(s) 113, 492
StyI CCWWGG 2 cut(s) 76, 100
TasI AATT 6 cut(s) 57, 141, 204, 364, 465, 583
TfiI GAWTC 1 cut(s) 175
Tru1I TTAA 6 cut(s) 66, 120, 147, 309, 561, 606
Tru9I TTAA 6 cut(s) 66, 120, 147, 309, 561, 606
TscAI CASTG 1 cut(s) 350
TseFI GTSAC 2 cut(s) 163, 241
Tsp45I GTSAC 2 cut(s) 163, 241
TspDTI ATGAA 3 cut(s) 170, 514, 582
TspRI CASTG 1 cut(s) 350
Van91I CCANNNNNTGG 1 cut(s) 389
VneI GTGCAC 1 cut(s) 543
VpaK11BI GGWCC 1 cut(s) 73
XapI RAATTY 1 cut(s) 57
XceI RCATGY 2 cut(s) 263, 277
XmaJI CCTAGG 1 cut(s) 100
XmnI GAANNNNTTC 1 cut(s) 85
XspI CTAG 2 cut(s) 101, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.