Rroxscaffold_4G00295190
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000357
Physical Location & Seq
Reverse (-)
15120441 .. 15124426
3986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00295190.1

Sequence Viewer

Length: 843 bp
ATGCTTTCTTATGCGCTACCCATGTCAATTCAGTCAGCAATTGACCTTGGCCTATTTGATATCATTTCCAAAGCGGGTACGGATGCCAAGCTCTCTGTATCAGATATTGCAGCCAAAATTGGCACCAACGAGGCGCCAGTTATGCTGGACCTCATCCTCAGGCTCCTCACCTCACACTCCATGCTCAGTTGCTCTGTGGTCAATGGCCAAAGGCTTTACAGCCTCACCGCGGTGTCCAAGCACTTTGCGACTAATGAAGATGGTGCTTCTTTAGGCCCCACCACAGCATTACTTCAATGCAATCTCAGCTTGAACTATTGGATCTATGGAAAAACCCCCTATCAGTATCAAGATTCAGACCCCAAGTTTAATCAACTTTTCAATTTGGGAATGGTCAACCTCACTACTTTGGTTATGAGGAGGATTCTTGATAGCTACCAGGGTTTTGAGCACCAACCAGGCGTGGAACATGTCGGAGGAGACATGTTTTCAAAGGTTCCATGTGGAGATGCCATTTTTCTGAAGCTTACTTTGAATATTTTGCAAAACGTACTTCATGATTGGATGGATGAGCAATGCATAAAATTATTGAAAAATTGTTTCACTGCGATTCCAGATAAAGGAAAAGTAATCGTTGTAGAATCACTTGCTTCAATTGAGCCAAACACTAGCCTTGCTGAGAAGATCTCCTCAGAGTTTGATGTGATTGTGATGACAGTAGCCCCGGGAGGGAAGGAAAGGACCCAACACCAATTTATGGACTTGGCAACTGCTGCTGGATTTAGTGGCATCAAATATGAATGTCTTTCCAGTTATCTTCATGTTATGGAGTTCATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

280

Amino Acids

30.81

Weight (kDa)

5.46

Isoelectric Point (pI)

27.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 2 - 84 1.3e-18 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 150 - 262 2.6e-27 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000357)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17810 FvH4_7g17811 FvH4_7g17820 FvH4_7g17830
malus_domestica MD00G1100700.v1.1 MD01G1089600.v1.1 MD01G1089800.v1.1 MD01G1090400.v1.1 MD01G1090500.v1.1 MD01G1090800.v1.1 MD04G1141100.v1.1 MD07G1161000.v1.1 MD07G1161100.v1.1 MD10G1030000.v1.1 MD10G1030200.v1.1 MD15G1409200.v1.1 MD15G1409700.v1.1
prunus_persica Prupe.2G199100_v2.0.a1 Prupe.2G199100_v2.0.a1 Prupe.2G199300_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199500_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199800_v2.0.a1 Prupe.2G200100_v2.0.a1 Prupe.2G200100_v2.0.a1
pyrus_communis pycom01g11360 pycom01g11370 pycom01g11400 pycom01g11440 pycom01g11480 pycom01g11490 pycom07g15690 pycom08g12100
rosa_chinensis RchiOBHm_Chr1g0317931 RchiOBHm_Chr1g0361211 RchiOBHm_Chr1g0361221 RchiOBHm_Chr1g0361241 RchiOBHm_Chr1g0367691 RchiOBHm_Chr4g0390171 RchiOBHm_Chr4g0396271 RchiOBHm_Chr6g0286421
rosa_laevigata RLG00000002555 RLG00000009555 RLG00000027275 RLG00000027731 RLG00000027732 RLG00000027734 RLG00000027738 RLG00000030575
rosa_multiflora Rmu_co8470451.1_g000001 Rmu_sc0000250.1_g000010 Rmu_sc0010379.1_g000006 Rmu_sc0011132.1_g000005 Rmu_sc0011132.1_g000007 Rmu_sc0038028.1_g000001 Rmu_ssc0000008.1_g000043 Rmu_ssc0000234.1_g000002 Rmu_ssc0000418.1_g000001
rosa_roxburghii Rroxscaffold_2G00116370 Rroxscaffold_4G00289300 Rroxscaffold_4G00295130 Rroxscaffold_4G00295140 Rroxscaffold_4G00295160 Rroxscaffold_4G00295190 Rroxscaffold_4G00295220 Rroxscaffold_4G00330100 Rroxscaffold_5G00341100
rosa_rugosa Rorug01G0287600 Rorug01G0287700 Rorug01G0336200 Rorug01G0336200 Rorug03G0350700
rosa_samantha Rh1AG030300 Rh1AG298500 Rh1AG298600 Rh1AG298700 Rh1AG298900 Rh1BG262400 Rh1BG262500 Rh1BG262800 Rh1BG304800 Rh1CG029000 Rh1CG279800 Rh1CG279900 Rh1CG280000 Rh1CG280100 Rh1CG280200 Rh1CG320500 Rh1DG041200 Rh1DG292700 Rh1DG292800 Rh1DG337100 Rh3DG107500 Rh4AG128100 Rh4BG024900 Rh4CG072700 Rh4DG063000 Rh6CG295900 Rh6DG287700
rosa_wichuraiana Rw1G002290 Rw1G026410 Rw1G026420 Rw1G026440 Rw4G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 122, 133
AccB7I CCANNNNNTGG 1 cut(s) 757
AccII CGCG 1 cut(s) 230
AciI CCGC 3 cut(s) 74, 228, 230
AclWI GGATC 1 cut(s) 329
AcoI YGGCCR 1 cut(s) 205
AcuI CTGAAG 1 cut(s) 542
AcyI GRCGYC 1 cut(s) 134
AfaI GTAC 2 cut(s) 79, 552
AfiI CCNNNNNNNGG 4 cut(s) 229, 620, 729, 757
AflIII ACRYGT 2 cut(s) 469, 483
AgsI TTSAA 7 cut(s) 296, 313, 382, 492, 535, 592, 654
AjnI CCWGG 2 cut(s) 438, 457
AleI CACNNNNGTG 1 cut(s) 230
AluBI AGCT 4 cut(s) 91, 309, 435, 526
AluI AGCT 4 cut(s) 91, 309, 435, 526
Alw21I GWGCWC 1 cut(s) 453
Alw26I GTCTC 1 cut(s) 474
AlwI GGATC 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 724
AoxI GGCC 3 cut(s) 49, 205, 274
ApeKI GCWGC 2 cut(s) 110, 773
AspLEI GCGC 2 cut(s) 16, 136
AspS9I GGNCC 3 cut(s) 148, 275, 741
AsuC2I CCSGG 2 cut(s) 725, 726
AsuHPI GGTGA 2 cut(s) 160, 217
AvaI CYCGRG 1 cut(s) 724
AvaII GGWCC 2 cut(s) 148, 741
AxyI CCTNAGG 1 cut(s) 158
BalI TGGCCA 1 cut(s) 207
BanI GGYRCC 2 cut(s) 122, 133
Bbv12I GWGCWC 1 cut(s) 453
BbvI GCAGC 2 cut(s) 122, 760
BccI CCATC 2 cut(s) 254, 559
BciT130I CCWGG 2 cut(s) 440, 459
BcnI CCSGG 2 cut(s) 725, 726
BcoDI GTCTC 1 cut(s) 474
BfaI CTAG 1 cut(s) 669
BfoI RGCGCY 1 cut(s) 137
BglII AGATCT 1 cut(s) 684
BisI GCNGC 2 cut(s) 111, 774
BlsI GCNGC 2 cut(s) 112, 775
Bme1390I CCNGG 4 cut(s) 440, 459, 725, 726
Bme18I GGWCC 2 cut(s) 148, 741
BmeT110I CYCGRG 1 cut(s) 724
BmgT120I GGNCC 3 cut(s) 148, 275, 741
BmiI GGNNCC 6 cut(s) 124, 135, 164, 277, 498, 743
BmrFI CCNGG 4 cut(s) 440, 459, 725, 726
BmsI GCATC 3 cut(s) 73, 499, 798
BplI GAGNNNNNCTC 2 cut(s) 671, 703
BpuMI CCSGG 2 cut(s) 725, 726
BsaHI GRCGYC 1 cut(s) 134
BsaJI CCNNGG 5 cut(s) 46, 228, 439, 723, 724
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 620, 729, 757
Bse1I ACTGG 2 cut(s) 137, 810
Bse21I CCTNAGG 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 581
BseBI CCWGG 2 cut(s) 440, 459
BseDI CCNNGG 5 cut(s) 46, 228, 439, 723, 724
BseGI GGATG 4 cut(s) 88, 153, 570, 574
BseLI CCNNNNNNNGG 4 cut(s) 229, 620, 729, 757
BseMI GCAATG 1 cut(s) 581
BseMII CTCAG 5 cut(s) 172, 199, 319, 669, 705
BseNI ACTGG 2 cut(s) 137, 810
BseRI GAGGAG 4 cut(s) 155, 433, 492, 679
BseXI GCAGC 2 cut(s) 122, 760
Bsh1236I CGCG 1 cut(s) 230
BshFI GGCC 3 cut(s) 51, 207, 276
BshNI GGYRCC 2 cut(s) 122, 133
BsiHKAI GWGCWC 1 cut(s) 453
BsiHKCI CYCGRG 1 cut(s) 724
BsiSI CCGG 1 cut(s) 725
BslI CCNNNNNNNGG 4 cut(s) 229, 620, 729, 757
BsmAI GTCTC 1 cut(s) 474
BsnI GGCC 3 cut(s) 51, 207, 276
BsoBI CYCGRG 1 cut(s) 724
Bsp1286I GDGCHC 1 cut(s) 453
Bsp143I GATC 2 cut(s) 321, 684
BspACI CCGC 3 cut(s) 74, 228, 230
BspANI GGCC 3 cut(s) 51, 207, 276
BspCNI CTCAG 5 cut(s) 171, 198, 318, 670, 704
BspFNI CGCG 1 cut(s) 230
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 6 cut(s) 124, 135, 164, 277, 498, 743
BspPI GGATC 1 cut(s) 329
BspT107I GGYRCC 2 cut(s) 122, 133
BsrDI GCAATG 1 cut(s) 581
BsrI ACTGG 2 cut(s) 137, 810
BssECI CCNNGG 5 cut(s) 46, 228, 439, 723, 724
BssMI GATC 2 cut(s) 321, 684
BssNI GRCGYC 1 cut(s) 134
BssT1I CCWWGG 1 cut(s) 46
Bst2UI CCWGG 2 cut(s) 440, 459
Bst4CI ACNGT 1 cut(s) 718
BstACI GRCGYC 1 cut(s) 134
BstAPI GCANNNNNTGC 1 cut(s) 773
BstDEI CTNAG 5 cut(s) 158, 185, 305, 678, 691
BstDSI CCRYGG 1 cut(s) 228
BstF5I GGATG 4 cut(s) 88, 153, 570, 574
BstFNI CGCG 1 cut(s) 230
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 2 cut(s) 16, 136
BstKTI GATC 2 cut(s) 324, 687
BstMAI GTCTC 1 cut(s) 474
BstMBI GATC 2 cut(s) 321, 684
BstMWI GCNNNNNNNGC 3 cut(s) 142, 306, 773
BstNI CCWGG 2 cut(s) 440, 459
BstNSI RCATGY 2 cut(s) 473, 487
BstSCI CCNGG 4 cut(s) 438, 457, 723, 724
BstUI CGCG 1 cut(s) 230
BstV1I GCAGC 2 cut(s) 122, 760
BstX2I RGATCY 2 cut(s) 321, 684
BstYI RGATCY 2 cut(s) 321, 684
Bsu36I CCTNAGG 1 cut(s) 158
BsuRI GGCC 3 cut(s) 51, 207, 276
BtgI CCRYGG 1 cut(s) 228
BtsCI GGATG 4 cut(s) 88, 153, 570, 574
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 1 cut(s) 603
CciI TCATGA 1 cut(s) 556
CfoI GCGC 2 cut(s) 16, 136
Cfr13I GGNCC 3 cut(s) 148, 275, 741
Cfr42I CCGCGG 1 cut(s) 231
Cfr9I CCCGGG 1 cut(s) 724
Csp6I GTAC 2 cut(s) 78, 551
CviAII CATG 7 cut(s) 22, 181, 470, 484, 501, 557, 821
CviQI GTAC 2 cut(s) 78, 551
DdeI CTNAG 5 cut(s) 158, 185, 305, 678, 691
DinI GGCGCC 1 cut(s) 135
DpnI GATC 2 cut(s) 323, 686
DpnII GATC 2 cut(s) 321, 684
EaeI YGGCCR 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 46
Eco32I GATATC 1 cut(s) 61
Eco47I GGWCC 2 cut(s) 148, 741
Eco57I CTGAAG 1 cut(s) 542
Eco81I CCTNAGG 1 cut(s) 158
Eco88I CYCGRG 1 cut(s) 724
EcoO109I RGGNCCY 2 cut(s) 275, 741
EcoRII CCWGG 2 cut(s) 438, 457
EcoRV GATATC 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 46
EcoT22I ATGCAT 1 cut(s) 581
EgeI GGCGCC 1 cut(s) 135
EheI GGCGCC 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 46
FaeI CATG 7 cut(s) 25, 184, 473, 487, 504, 560, 824
FatI CATG 7 cut(s) 21, 180, 469, 483, 500, 556, 820
FauI CCCGC 1 cut(s) 67
Fnu4HI GCNGC 2 cut(s) 111, 774
FokI GGATG 4 cut(s) 95, 140, 577, 581
Fsp4HI GCNGC 2 cut(s) 111, 774
FspBI CTAG 1 cut(s) 669
GlaI GCGC 2 cut(s) 15, 135
GluI GCNGC 2 cut(s) 111, 774
HaeII RGCGCY 1 cut(s) 137
HaeIII GGCC 3 cut(s) 51, 207, 276
HapII CCGG 1 cut(s) 725
HhaI GCGC 2 cut(s) 16, 136
Hin1I GRCGYC 1 cut(s) 134
Hin1II CATG 7 cut(s) 25, 184, 473, 487, 504, 560, 824
Hin6I GCGC 2 cut(s) 14, 134
HinP1I GCGC 2 cut(s) 14, 134
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HindIII AAGCTT 1 cut(s) 524
HinfI GANTC 4 cut(s) 353, 424, 610, 641
HpaII CCGG 1 cut(s) 725
HphI GGTGA 2 cut(s) 160, 217
Hpy166II GTNNAC 1 cut(s) 397
Hpy188I TCNGA 5 cut(s) 103, 358, 476, 522, 694
Hpy188III TCNNGA 4 cut(s) 350, 428, 557, 614
Hpy8I GTNNAC 1 cut(s) 397
HpyAV CCTTC 1 cut(s) 727
HpyCH4III ACNGT 1 cut(s) 718
HpyCH4IV ACGT 1 cut(s) 549
HpyCH4V TGCA 4 cut(s) 110, 300, 544, 579
HpyF10VI GCNNNNNNNGC 3 cut(s) 142, 306, 773
HpyF3I CTNAG 5 cut(s) 158, 185, 305, 678, 691
HpySE526I ACGT 1 cut(s) 549
Hsp92I GRCGYC 1 cut(s) 134
Hsp92II CATG 7 cut(s) 25, 184, 473, 487, 504, 560, 824
HspAI GCGC 2 cut(s) 14, 134
KasI GGCGCC 1 cut(s) 133
KspI CCGCGG 1 cut(s) 231
Kzo9I GATC 2 cut(s) 321, 684
LmnI GCTCC 1 cut(s) 168
Lsp1109I GCAGC 2 cut(s) 122, 760
LweI GCATC 3 cut(s) 73, 499, 798
MaeI CTAG 1 cut(s) 669
MaeII ACGT 1 cut(s) 549
MalI GATC 2 cut(s) 323, 686
MboI GATC 2 cut(s) 321, 684
MboII GAAGA 3 cut(s) 269, 694, 809
MfeI CAATTG 2 cut(s) 39, 654
MflI RGATCY 2 cut(s) 321, 684
MhlI GDGCHC 1 cut(s) 453
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 8 cut(s) 27, 39, 117, 382, 584, 595, 654, 752
MluNI TGGCCA 1 cut(s) 207
Mly113I GGCGCC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 454
Mox20I TGGCCA 1 cut(s) 207
Mph1103I ATGCAT 1 cut(s) 581
MscI TGGCCA 1 cut(s) 207
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 1 cut(s) 230
Msp20I TGGCCA 1 cut(s) 207
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 1 cut(s) 725
MspR9I CCNGG 4 cut(s) 440, 459, 725, 726
MunI CAATTG 2 cut(s) 39, 654
MvaI CCWGG 2 cut(s) 440, 459
MvnI CGCG 1 cut(s) 230
MwoI GCNNNNNNNGC 3 cut(s) 142, 306, 773
NarI GGCGCC 1 cut(s) 134
NciI CCSGG 2 cut(s) 725, 726
NdeII GATC 2 cut(s) 321, 684
NlaIII CATG 7 cut(s) 25, 184, 473, 487, 504, 560, 824
NlaIV GGNNCC 6 cut(s) 124, 135, 164, 277, 498, 743
NsiI ATGCAT 1 cut(s) 581
NspI RCATGY 2 cut(s) 473, 487
OliI CACNNNNGTG 1 cut(s) 230
PagI TCATGA 1 cut(s) 556
PciI ACATGT 2 cut(s) 469, 483
PfeI GAWTC 4 cut(s) 353, 424, 610, 641
PflMI CCANNNNNTGG 1 cut(s) 757
PkrI GCNGC 2 cut(s) 112, 775
PluTI GGCGCC 1 cut(s) 137
PpuMI RGGWCCY 1 cut(s) 741
PscI ACATGT 2 cut(s) 469, 483
Psp5II RGGWCCY 1 cut(s) 741
Psp6I CCWGG 2 cut(s) 438, 457
PspGI CCWGG 2 cut(s) 438, 457
PspN4I GGNNCC 6 cut(s) 124, 135, 164, 277, 498, 743
PspPI GGNCC 3 cut(s) 148, 275, 741
PspPPI RGGWCCY 1 cut(s) 741
PsuI RGATCY 2 cut(s) 321, 684
RsaI GTAC 2 cut(s) 79, 552
RsaNI GTAC 2 cut(s) 78, 551
RseI CAYNNNNRTG 1 cut(s) 230
SacII CCGCGG 1 cut(s) 231
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 2 cut(s) 111, 774
Sau3AI GATC 2 cut(s) 321, 684
Sau96I GGNCC 3 cut(s) 148, 275, 741
ScrFI CCNGG 4 cut(s) 440, 459, 725, 726
SduI GDGCHC 1 cut(s) 453
SfaNI GCATC 3 cut(s) 73, 499, 798
SfoI GGCGCC 1 cut(s) 135
Sfr303I CCGCGG 1 cut(s) 231
SgrBI CCGCGG 1 cut(s) 231
SinI GGWCC 2 cut(s) 148, 741
SmaI CCCGGG 1 cut(s) 726
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 8 cut(s) 27, 39, 117, 382, 584, 595, 654, 752
SsiI CCGC 3 cut(s) 74, 228, 230
SspDI GGCGCC 1 cut(s) 133
SspI AATATT 1 cut(s) 538
SspMI CTAG 1 cut(s) 669
StyD4I CCNGG 4 cut(s) 438, 457, 723, 724
StyI CCWWGG 1 cut(s) 46
TaaI ACNGT 1 cut(s) 718
TaiI ACGT 1 cut(s) 552
TasI AATT 8 cut(s) 27, 39, 117, 382, 584, 595, 654, 752
TfiI GAWTC 4 cut(s) 353, 424, 610, 641
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 1 cut(s) 610
TseI GCWGC 2 cut(s) 110, 773
TspDTI ATGAA 5 cut(s) 270, 545, 809, 813, 823
TspGWI ACGGA 1 cut(s) 95
TspMI CCCGGG 1 cut(s) 724
TspRI CASTG 1 cut(s) 610
Van91I CCANNNNNTGG 1 cut(s) 757
VpaK11BI GGWCC 2 cut(s) 148, 741
XceI RCATGY 2 cut(s) 473, 487
XmaI CCCGGG 1 cut(s) 724
XspI CTAG 1 cut(s) 669
Zsp2I ATGCAT 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.