Rroxscaffold_4G00330100
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000357
Physical Location & Seq
Forward (+)
63495322 .. 63497228
1907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00330100.1

Sequence Viewer

Length: 999 bp
ATGAGCACAGACAGTTTTAAGCCAGTAATGACGTCCACAAAAGAAGAAAAAGAAGAAGAAAGCTTCAGCTATGCCATGCAGCTGGTGACTTCTAATGTGCTGCCTATGTCCATGCAATCAGCAGTTGAGCTTGGAGTTTTCGACATCATAGCCAAAGCTGGTGCGGGTGCGGGTGTGGGTCTCTCCTCATCCCAGATTGCAGCTCAGCTAGGCATCCAGAACCCCGAGGCGCCCATGATGTTGGATCGAATACTCAGGCTATTGGCAAGTCACTCTGTTCTCAATTGCACTGTGGTTAATGCTAATGATGATGATCATGTTGATGGGCCTAATTTTCAGGGGGTTTATAGTCTTGCTCCTGTGTCCAATTACTTTGTGAAGAGTGATGAAGATGGTGTTTCTTTAGGCGCCCTGATGGCATTGACTCAAGACAAGGTCTTCTTGGACAGCTGGTTCCAGTTGAAAGGTGCTGTTGTGGAAGGAGGAATCCCATTTAACAGGGTCCATGGCACACATGCCTTTGAGTATCCAGGTTTGGACCCCAGGTTTAACCAAGTGTTCAACACAGCAATGTGCAACCACACCACTATTGTTATCAAGAAAATTCTTGATCTGTACAAGGGTTTTGAGCACCTTAAGCAACTTGTTGATGTTGGGGGTGGCCTTGGAGTGACTCTTAGCCTAATCACTTCTAGATACCCTCATATTAAGGGCATCAACTTTGACTTGCCTCATGTCATCAAAGATGCCCCTTCTTACACTGGTATAACTTTAAGAGTGGAACATGTTGGAGGAGACATGTTTGCAAGTGTTCCAAGTGGGGATGCCATTTTTATGAAGTGGATACTTCATGACTGGAATGATGAGCACTGCTTGAAGCTTCTGAAAAATTGTTACAATGCCATACCAGACAATGGAAAAATAATTGTTGTGGAAGCACTTCTTCCAGTTTTGCCAGGAACCATACTGCTGCAAAGAGCACCTGCCAAATGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01058 ko01100 ko01110 map00940 map01058 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

332

Amino Acids

36.25

Weight (kDa)

5.51

Isoelectric Point (pI)

28.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 26 - 128 1.4e-18 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 151 - 319 3.3e-58 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000357)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17810 FvH4_7g17811 FvH4_7g17820 FvH4_7g17830
malus_domestica MD00G1100700.v1.1 MD01G1089600.v1.1 MD01G1089800.v1.1 MD01G1090400.v1.1 MD01G1090500.v1.1 MD01G1090800.v1.1 MD04G1141100.v1.1 MD07G1161000.v1.1 MD07G1161100.v1.1 MD10G1030000.v1.1 MD10G1030200.v1.1 MD15G1409200.v1.1 MD15G1409700.v1.1
prunus_persica Prupe.2G199100_v2.0.a1 Prupe.2G199100_v2.0.a1 Prupe.2G199300_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199500_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199800_v2.0.a1 Prupe.2G200100_v2.0.a1 Prupe.2G200100_v2.0.a1
pyrus_communis pycom01g11360 pycom01g11370 pycom01g11400 pycom01g11440 pycom01g11480 pycom01g11490 pycom07g15690 pycom08g12100
rosa_chinensis RchiOBHm_Chr1g0317931 RchiOBHm_Chr1g0361211 RchiOBHm_Chr1g0361221 RchiOBHm_Chr1g0361241 RchiOBHm_Chr1g0367691 RchiOBHm_Chr4g0390171 RchiOBHm_Chr4g0396271 RchiOBHm_Chr6g0286421
rosa_laevigata RLG00000002555 RLG00000009555 RLG00000027275 RLG00000027731 RLG00000027732 RLG00000027734 RLG00000027738 RLG00000030575
rosa_multiflora Rmu_co8470451.1_g000001 Rmu_sc0000250.1_g000010 Rmu_sc0010379.1_g000006 Rmu_sc0011132.1_g000005 Rmu_sc0011132.1_g000007 Rmu_sc0038028.1_g000001 Rmu_ssc0000008.1_g000043 Rmu_ssc0000234.1_g000002 Rmu_ssc0000418.1_g000001
rosa_roxburghii Rroxscaffold_2G00116370 Rroxscaffold_4G00289300 Rroxscaffold_4G00295130 Rroxscaffold_4G00295140 Rroxscaffold_4G00295160 Rroxscaffold_4G00295190 Rroxscaffold_4G00295220 Rroxscaffold_4G00330100 Rroxscaffold_5G00341100
rosa_rugosa Rorug01G0287600 Rorug01G0287700 Rorug01G0336200 Rorug01G0336200 Rorug03G0350700
rosa_samantha Rh1AG030300 Rh1AG298500 Rh1AG298600 Rh1AG298700 Rh1AG298900 Rh1BG262400 Rh1BG262500 Rh1BG262800 Rh1BG304800 Rh1CG029000 Rh1CG279800 Rh1CG279900 Rh1CG280000 Rh1CG280100 Rh1CG280200 Rh1CG320500 Rh1DG041200 Rh1DG292700 Rh1DG292800 Rh1DG337100 Rh3DG107500 Rh4AG128100 Rh4BG024900 Rh4CG072700 Rh4DG063000 Rh6CG295900 Rh6DG287700
rosa_wichuraiana Rw1G002290 Rw1G026410 Rw1G026420 Rw1G026440 Rw4G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 991
AatII GACGTC 1 cut(s) 35
Acc36I ACCTGC 1 cut(s) 991
AccB1I GGYRCC 2 cut(s) 229, 407
AccB7I CCANNNNNTGG 1 cut(s) 914
AciI CCGC 2 cut(s) 164, 170
AclWI GGATC 1 cut(s) 252
AcsI RAATTY 1 cut(s) 603
AcuI CTGAAG 1 cut(s) 49
AcyI GRCGYC 3 cut(s) 32, 230, 408
AfaI GTAC 1 cut(s) 617
AfiI CCNNNNNNNGG 1 cut(s) 914
AflII CTTAAG 1 cut(s) 635
AflIII ACRYGT 2 cut(s) 784, 798
AgsI TTSAA 3 cut(s) 463, 562, 877
AjnI CCWGG 3 cut(s) 529, 542, 955
AloI GAACNNNNNNTCC 2 cut(s) 437, 469
AluBI AGCT 9 cut(s) 63, 69, 82, 130, 158, 203, 208, 450, 880
AluI AGCT 9 cut(s) 63, 69, 82, 130, 158, 203, 208, 450, 880
Alw21I GWGCWC 4 cut(s) 8, 633, 870, 982
Alw26I GTCTC 2 cut(s) 185, 789
AlwI GGATC 1 cut(s) 252
Ama87I CYCGRG 1 cut(s) 224
AoxI GGCC 2 cut(s) 326, 661
ApeKI GCWGC 4 cut(s) 79, 100, 200, 970
ApoI RAATTY 1 cut(s) 603
Asp700I GAANNNNTTC 1 cut(s) 939
AspLEI GCGC 2 cut(s) 232, 410
AspS9I GGNCC 3 cut(s) 326, 502, 538
AsuHPI GGTGA 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 224
AvaII GGWCC 2 cut(s) 502, 538
BanI GGYRCC 2 cut(s) 229, 407
BbsI GAAGAC 1 cut(s) 430
Bbv12I GWGCWC 4 cut(s) 8, 633, 870, 982
BbvI GCAGC 4 cut(s) 87, 91, 212, 957
BccI CCATC 3 cut(s) 317, 386, 409
BciT130I CCWGG 3 cut(s) 531, 544, 957
BciVI GTATCC 2 cut(s) 537, 837
BclI TGATCA 1 cut(s) 313
BcoDI GTCTC 2 cut(s) 185, 789
BfaI CTAG 2 cut(s) 209, 693
BfoI RGCGCY 2 cut(s) 233, 411
BfrI CTTAAG 1 cut(s) 635
BfuAI ACCTGC 1 cut(s) 991
BfuI GTATCC 2 cut(s) 537, 837
BglI GCCNNNNNGGC 1 cut(s) 416
BisI GCNGC 4 cut(s) 80, 101, 201, 971
BlpI GCTNAGC 1 cut(s) 204
BlsI GCNGC 4 cut(s) 81, 102, 202, 972
Bme1390I CCNGG 3 cut(s) 531, 544, 957
Bme18I GGWCC 2 cut(s) 502, 538
BmeT110I CYCGRG 1 cut(s) 224
BmgT120I GGNCC 3 cut(s) 326, 502, 538
BmiI GGNNCC 6 cut(s) 231, 409, 455, 503, 540, 961
BmrFI CCNGG 3 cut(s) 531, 544, 957
BmsI GCATC 4 cut(s) 222, 723, 736, 814
BpiI GAAGAC 1 cut(s) 430
Bpu1102I GCTNAGC 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 411
BsaBI GATNNNNATC 1 cut(s) 312
BsaHI GRCGYC 3 cut(s) 32, 230, 408
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 4 cut(s) 225, 505, 542, 664
Bsc4I CCNNNNNNNGG 1 cut(s) 914
Bse1I ACTGG 5 cut(s) 23, 457, 766, 860, 947
Bse3DI GCAATG 1 cut(s) 576
Bse8I GATNNNNATC 1 cut(s) 312
BseBI CCWGG 3 cut(s) 531, 544, 957
BseDI CCNNGG 4 cut(s) 225, 505, 542, 664
BseGI GGATG 4 cut(s) 188, 213, 829, 999
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 1 cut(s) 914
BseMI GCAATG 1 cut(s) 576
BseMII CTCAG 2 cut(s) 218, 268
BseNI ACTGG 5 cut(s) 23, 457, 766, 860, 947
BseRI GAGGAG 2 cut(s) 175, 807
BseXI GCAGC 4 cut(s) 87, 91, 212, 957
BshFI GGCC 2 cut(s) 328, 663
BshNI GGYRCC 2 cut(s) 229, 407
BsiHKAI GWGCWC 4 cut(s) 8, 633, 870, 982
BsiHKCI CYCGRG 1 cut(s) 224
BslI CCNNNNNNNGG 1 cut(s) 914
BsmAI GTCTC 2 cut(s) 185, 789
BsnI GGCC 2 cut(s) 328, 663
Bso31I GGTCTC 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 224
Bsp1286I GDGCHC 4 cut(s) 8, 633, 870, 982
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 3 cut(s) 244, 313, 610
Bsp1720I GCTNAGC 1 cut(s) 204
Bsp19I CCATGG 1 cut(s) 505
BspACI CCGC 2 cut(s) 164, 170
BspANI GGCC 2 cut(s) 328, 663
BspCNI CTCAG 2 cut(s) 217, 267
BspHI TCATGA 1 cut(s) 850
BspLI GGNNCC 6 cut(s) 231, 409, 455, 503, 540, 961
BspMI ACCTGC 1 cut(s) 991
BspPI GGATC 1 cut(s) 252
BspT107I GGYRCC 2 cut(s) 229, 407
BspTI CTTAAG 1 cut(s) 635
BspTNI GGTCTC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 576
BsrGI TGTACA 1 cut(s) 615
BsrI ACTGG 5 cut(s) 23, 457, 766, 860, 947
BssECI CCNNGG 4 cut(s) 225, 505, 542, 664
BssMI GATC 3 cut(s) 244, 313, 610
BssNI GRCGYC 3 cut(s) 32, 230, 408
BssT1I CCWWGG 2 cut(s) 505, 664
Bst2UI CCWGG 3 cut(s) 531, 544, 957
Bst4CI ACNGT 2 cut(s) 14, 292
Bst6I CTCTTC 1 cut(s) 374
BstACI GRCGYC 3 cut(s) 32, 230, 408
BstAFI CTTAAG 1 cut(s) 635
BstAUI TGTACA 1 cut(s) 615
BstDEI CTNAG 3 cut(s) 204, 254, 677
BstDSI CCRYGG 1 cut(s) 505
BstF5I GGATG 4 cut(s) 188, 213, 829, 999
BstH2I RGCGCY 2 cut(s) 233, 411
BstHHI GCGC 2 cut(s) 232, 410
BstKTI GATC 3 cut(s) 247, 316, 613
BstMAI GTCTC 2 cut(s) 185, 789
BstMBI GATC 3 cut(s) 244, 313, 610
BstMWI GCNNNNNNNGC 2 cut(s) 416, 637
BstNI CCWGG 3 cut(s) 531, 544, 957
BstNSI RCATGY 3 cut(s) 518, 788, 802
BstSCI CCNGG 3 cut(s) 529, 542, 955
BstV1I GCAGC 4 cut(s) 87, 91, 212, 957
BstV2I GAAGAC 1 cut(s) 430
BstXI CCANNNNNNTGG 2 cut(s) 82, 241
BsuI GTATCC 2 cut(s) 537, 837
BsuRI GGCC 2 cut(s) 328, 663
BtgI CCRYGG 1 cut(s) 505
BtsCI GGATG 4 cut(s) 188, 213, 829, 999
BtsI GCAGTG 1 cut(s) 868
BtsIMutI CAGTG 3 cut(s) 288, 759, 868
BveI ACCTGC 1 cut(s) 991
CciI TCATGA 1 cut(s) 850
CfoI GCGC 2 cut(s) 232, 410
Cfr13I GGNCC 3 cut(s) 326, 502, 538
Csp6I GTAC 1 cut(s) 616
CviQI GTAC 1 cut(s) 616
DdeI CTNAG 3 cut(s) 204, 254, 677
DinI GGCGCC 2 cut(s) 231, 409
DpnI GATC 3 cut(s) 246, 315, 612
DpnII GATC 3 cut(s) 244, 313, 610
Eam1104I CTCTTC 1 cut(s) 374
EarI CTCTTC 1 cut(s) 374
Eco130I CCWWGG 2 cut(s) 505, 664
Eco31I GGTCTC 1 cut(s) 185
Eco47I GGWCC 2 cut(s) 502, 538
Eco57I CTGAAG 1 cut(s) 49
Eco88I CYCGRG 1 cut(s) 224
EcoRII CCWGG 3 cut(s) 529, 542, 955
EcoT14I CCWWGG 2 cut(s) 505, 664
EgeI GGCGCC 2 cut(s) 231, 409
EheI GGCGCC 2 cut(s) 231, 409
ErhI CCWWGG 2 cut(s) 505, 664
FalI AAGNNNNNCTT 4 cut(s) 425, 457, 927, 959
FauI CCCGC 2 cut(s) 157, 163
FbaI TGATCA 1 cut(s) 313
Fnu4HI GCNGC 4 cut(s) 80, 101, 201, 971
FokI GGATG 3 cut(s) 175, 200, 836
Fsp4HI GCNGC 4 cut(s) 80, 101, 201, 971
FspBI CTAG 2 cut(s) 209, 693
GlaI GCGC 2 cut(s) 231, 409
GluI GCNGC 4 cut(s) 80, 101, 201, 971
HaeII RGCGCY 2 cut(s) 233, 411
HaeIII GGCC 2 cut(s) 328, 663
HhaI GCGC 2 cut(s) 232, 410
Hin1I GRCGYC 3 cut(s) 32, 230, 408
Hin6I GCGC 2 cut(s) 230, 408
HinP1I GCGC 2 cut(s) 230, 408
HindIII AAGCTT 2 cut(s) 61, 878
HinfI GANTC 3 cut(s) 424, 486, 673
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 1 cut(s) 36
Hpy188I TCNGA 1 cut(s) 885
Hpy188III TCNNGA 6 cut(s) 217, 428, 598, 608, 693, 851
Hpy8I GTNNAC 1 cut(s) 36
HpyAV CCTTC 2 cut(s) 473, 762
HpyCH4III ACNGT 2 cut(s) 14, 292
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 7 cut(s) 79, 115, 200, 288, 576, 806, 973
HpyF10VI GCNNNNNNNGC 2 cut(s) 416, 637
HpyF3I CTNAG 3 cut(s) 204, 254, 677
HpySE526I ACGT 1 cut(s) 32
Hsp92I GRCGYC 3 cut(s) 32, 230, 408
HspAI GCGC 2 cut(s) 230, 408
KasI GGCGCC 2 cut(s) 229, 407
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 3 cut(s) 244, 313, 610
LmnI GCTCC 1 cut(s) 361
Lsp1109I GCAGC 4 cut(s) 87, 91, 212, 957
LweI GCATC 4 cut(s) 222, 723, 736, 814
MaeI CTAG 2 cut(s) 209, 693
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 4 cut(s) 85, 269, 670, 893
MalI GATC 3 cut(s) 246, 315, 612
MboI GATC 3 cut(s) 244, 313, 610
MboII GAAGA 7 cut(s) 56, 65, 68, 391, 401, 430, 935
MfeI CAATTG 1 cut(s) 283
MhlI GDGCHC 4 cut(s) 8, 633, 870, 982
MluCI AATT 6 cut(s) 283, 331, 367, 603, 889, 924
Mly113I GGCGCC 2 cut(s) 230, 408
MlyI GAGTC 2 cut(s) 418, 667
MmeI TCCRAC 2 cut(s) 222, 769
MnlI CCTC 6 cut(s) 196, 220, 476, 711, 741, 785
MroXI GAANNNNTTC 1 cut(s) 939
MseI TTAA 7 cut(s) 18, 297, 495, 549, 636, 708, 773
MslI CAYNNNNRTG 3 cut(s) 321, 569, 833
MspA1I CMGCKG 2 cut(s) 82, 450
MspCI CTTAAG 1 cut(s) 635
MspR9I CCNGG 3 cut(s) 531, 544, 957
MunI CAATTG 1 cut(s) 283
MvaI CCWGG 3 cut(s) 531, 544, 957
MwoI GCNNNNNNNGC 2 cut(s) 416, 637
NarI GGCGCC 2 cut(s) 230, 408
NcoI CCATGG 1 cut(s) 505
NdeII GATC 3 cut(s) 244, 313, 610
NlaIV GGNNCC 6 cut(s) 231, 409, 455, 503, 540, 961
NmuCI GTSAC 3 cut(s) 85, 269, 670
NspI RCATGY 3 cut(s) 518, 788, 802
PagI TCATGA 1 cut(s) 850
PaqCI CACCTGC 1 cut(s) 991
PciI ACATGT 2 cut(s) 784, 798
PdmI GAANNNNTTC 1 cut(s) 939
PfeI GAWTC 1 cut(s) 486
PflFI GACNNNGTC 1 cut(s) 434
PflMI CCANNNNNTGG 1 cut(s) 914
PkrI GCNGC 4 cut(s) 81, 102, 202, 972
PleI GAGTC 2 cut(s) 418, 667
PluTI GGCGCC 2 cut(s) 233, 411
PpsI GAGTC 2 cut(s) 418, 667
PscI ACATGT 2 cut(s) 784, 798
Psp6I CCWGG 3 cut(s) 529, 542, 955
PspGI CCWGG 3 cut(s) 529, 542, 955
PspN4I GGNNCC 6 cut(s) 231, 409, 455, 503, 540, 961
PspPI GGNCC 3 cut(s) 326, 502, 538
PsyI GACNNNGTC 1 cut(s) 434
PvuII CAGCTG 2 cut(s) 82, 450
RsaI GTAC 1 cut(s) 617
RsaNI GTAC 1 cut(s) 616
RseI CAYNNNNRTG 3 cut(s) 321, 569, 833
SaqAI TTAA 7 cut(s) 18, 297, 495, 549, 636, 708, 773
SatI GCNGC 4 cut(s) 80, 101, 201, 971
Sau3AI GATC 3 cut(s) 244, 313, 610
Sau96I GGNCC 3 cut(s) 326, 502, 538
SchI GAGTC 2 cut(s) 418, 667
ScrFI CCNGG 3 cut(s) 531, 544, 957
SduI GDGCHC 4 cut(s) 8, 633, 870, 982
SfaNI GCATC 4 cut(s) 222, 723, 736, 814
SfoI GGCGCC 2 cut(s) 231, 409
SinI GGWCC 2 cut(s) 502, 538
SmiMI CAYNNNNRTG 3 cut(s) 321, 569, 833
SmlI CTYRAG 2 cut(s) 426, 635
SmoI CTYRAG 2 cut(s) 426, 635
Sse9I AATT 6 cut(s) 283, 331, 367, 603, 889, 924
SsiI CCGC 2 cut(s) 164, 170
SspDI GGCGCC 2 cut(s) 229, 407
SspMI CTAG 2 cut(s) 209, 693
StyD4I CCNGG 3 cut(s) 529, 542, 955
StyI CCWWGG 2 cut(s) 505, 664
TaaI ACNGT 2 cut(s) 14, 292
TaiI ACGT 1 cut(s) 35
TaqI TCGA 2 cut(s) 141, 247
TasI AATT 6 cut(s) 283, 331, 367, 603, 889, 924
TatI WGTACW 1 cut(s) 615
TfiI GAWTC 1 cut(s) 486
Tru1I TTAA 7 cut(s) 18, 297, 495, 549, 636, 708, 773
Tru9I TTAA 7 cut(s) 18, 297, 495, 549, 636, 708, 773
TscAI CASTG 3 cut(s) 295, 766, 875
TseFI GTSAC 3 cut(s) 85, 269, 670
TseI GCWGC 4 cut(s) 79, 100, 200, 970
Tsp45I GTSAC 3 cut(s) 85, 269, 670
TspDTI ATGAA 3 cut(s) 402, 839, 851
TspRI CASTG 3 cut(s) 295, 766, 875
Tth111I GACNNNGTC 1 cut(s) 434
Van91I CCANNNNNTGG 1 cut(s) 914
Vha464I CTTAAG 1 cut(s) 635
VpaK11BI GGWCC 2 cut(s) 502, 538
XapI RAATTY 1 cut(s) 603
XbaI TCTAGA 1 cut(s) 692
XceI RCATGY 3 cut(s) 518, 788, 802
XmnI GAANNNNTTC 1 cut(s) 939
XspI CTAG 2 cut(s) 209, 693
ZraI GACGTC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.