Rh4CG072700
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000357
Physical Location & Seq
Reverse (-)
13419629 .. 13421150
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG072700.1

Sequence Viewer

Length: 711 bp
ATGGCATCAATTCAAGCCAATGTCGTTATGAACAGTTGGTCACAAGTGAAAGATGCAATTGTTGAAGGAGGAATTCCGTTTAATAGGGTCCATGGCAAACACTTCTTTGAGTATACAGATTCGGACCCTAGGTTTAATCAAGTTTTTAATTCGGGAATGGTCAACCTCACCACTTTGGTTATGAGGAGGATTCTTGATAGCTACCAGGGTTTTGAGCACCTCACCCAGGTTGTTGATGTTGGGGGAGGGCTAGGGGTGGCTCTTGGTCTCATCACTTCCAGATATCCTCATGTTAAGGGAATCAACTACGACTTGCCCCATGTTATAAAACATGCTCCCCATTATCCTGGTGTGCAACATGTAGGAGGAGACATGTTTTCAAATGTTCCAAGTGGGGATGCCATTTTTATGAAGAACATACTTCATGACTGGATGGATGAGCAGTGCATAAAGCTATTGAAAAATTGTTACACAGCGATTCCAGATAATGGAAAAGTAATAGTTGTGGAAGCACTAGTTTCGGTTGAACCAGACACTAGCCCAGCTGAGAAGATCACCTCAGATTTTGATGTGCTTATGATGACACTATCCCCAGGAGGGAAGGAAAGGACCCAACACGAATTTATGGACTTAGCAAGTGCTGCTGGATTTAGTGGCATTAAATATGAATGCCTTTCCAGTTATCTTCGTGTTATGGTGTTCATAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01058 ko01100 ko01110 map00940 map01058 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

236

Amino Acids

26.17

Weight (kDa)

5.89

Isoelectric Point (pI)

26.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 13 - 218 2.4e-66 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000357)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17810 FvH4_7g17811 FvH4_7g17820 FvH4_7g17830
malus_domestica MD00G1100700.v1.1 MD01G1089600.v1.1 MD01G1089800.v1.1 MD01G1090400.v1.1 MD01G1090500.v1.1 MD01G1090800.v1.1 MD04G1141100.v1.1 MD07G1161000.v1.1 MD07G1161100.v1.1 MD10G1030000.v1.1 MD10G1030200.v1.1 MD15G1409200.v1.1 MD15G1409700.v1.1
prunus_persica Prupe.2G199100_v2.0.a1 Prupe.2G199100_v2.0.a1 Prupe.2G199300_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199400_v2.0.a1 Prupe.2G199500_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199600_v2.0.a1 Prupe.2G199800_v2.0.a1 Prupe.2G200100_v2.0.a1 Prupe.2G200100_v2.0.a1
pyrus_communis pycom01g11360 pycom01g11370 pycom01g11400 pycom01g11440 pycom01g11480 pycom01g11490 pycom07g15690 pycom08g12100
rosa_chinensis RchiOBHm_Chr1g0317931 RchiOBHm_Chr1g0361211 RchiOBHm_Chr1g0361221 RchiOBHm_Chr1g0361241 RchiOBHm_Chr1g0367691 RchiOBHm_Chr4g0390171 RchiOBHm_Chr4g0396271 RchiOBHm_Chr6g0286421
rosa_laevigata RLG00000002555 RLG00000009555 RLG00000027275 RLG00000027731 RLG00000027732 RLG00000027734 RLG00000027738 RLG00000030575
rosa_multiflora Rmu_co8470451.1_g000001 Rmu_sc0000250.1_g000010 Rmu_sc0010379.1_g000006 Rmu_sc0011132.1_g000005 Rmu_sc0011132.1_g000007 Rmu_sc0038028.1_g000001 Rmu_ssc0000008.1_g000043 Rmu_ssc0000234.1_g000002 Rmu_ssc0000418.1_g000001
rosa_roxburghii Rroxscaffold_2G00116370 Rroxscaffold_4G00289300 Rroxscaffold_4G00295130 Rroxscaffold_4G00295140 Rroxscaffold_4G00295160 Rroxscaffold_4G00295190 Rroxscaffold_4G00295220 Rroxscaffold_4G00330100 Rroxscaffold_5G00341100
rosa_rugosa Rorug01G0287600 Rorug01G0287700 Rorug01G0336200 Rorug01G0336200 Rorug03G0350700
rosa_samantha Rh1AG030300 Rh1AG298500 Rh1AG298600 Rh1AG298700 Rh1AG298900 Rh1BG262400 Rh1BG262500 Rh1BG262800 Rh1BG304800 Rh1CG029000 Rh1CG279800 Rh1CG279900 Rh1CG280000 Rh1CG280100 Rh1CG280200 Rh1CG320500 Rh1DG041200 Rh1DG292700 Rh1DG292800 Rh1DG337100 Rh3DG107500 Rh4AG128100 Rh4BG024900 Rh4CG072700 Rh4DG063000 Rh6CG295900 Rh6DG287700
rosa_wichuraiana Rw1G002290 Rw1G026410 Rw1G026420 Rw1G026440 Rw4G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 326
AccB7I CCANNNNNTGG 1 cut(s) 488
AccI GTMKAC 1 cut(s) 113
AcsI RAATTY 2 cut(s) 72, 620
AfiI CCNNNNNNNGG 3 cut(s) 226, 488, 597
AflIII ACRYGT 2 cut(s) 358, 372
AgsI TTSAA 5 cut(s) 14, 65, 381, 460, 527
AhlI ACTAGT 1 cut(s) 514
AjnI CCWGG 4 cut(s) 204, 225, 346, 592
AluBI AGCT 3 cut(s) 201, 454, 545
AluI AGCT 3 cut(s) 201, 454, 545
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 2 cut(s) 272, 363
ApeKI GCWGC 1 cut(s) 641
ApoI RAATTY 2 cut(s) 72, 620
AspA2I CCTAGG 1 cut(s) 128
AspS9I GGNCC 3 cut(s) 88, 124, 609
AsuHPI GGTGA 3 cut(s) 160, 214, 547
AvaII GGWCC 3 cut(s) 88, 124, 609
AvrII CCTAGG 1 cut(s) 128
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 1 cut(s) 628
BccI CCATC 1 cut(s) 427
BcgI CGANNNNNNTGC 2 cut(s) 501, 535
BciT130I CCWGG 4 cut(s) 206, 227, 348, 594
BcoDI GTCTC 2 cut(s) 272, 363
BcuI ACTAGT 1 cut(s) 514
BfaI CTAG 4 cut(s) 129, 251, 515, 537
BisI GCNGC 1 cut(s) 642
BlnI CCTAGG 1 cut(s) 128
BlsI GCNGC 1 cut(s) 643
Bme1390I CCNGG 4 cut(s) 206, 227, 348, 594
Bme18I GGWCC 3 cut(s) 88, 124, 609
BmgT120I GGNCC 3 cut(s) 88, 124, 609
BmiI GGNNCC 3 cut(s) 89, 126, 611
BmrFI CCNGG 4 cut(s) 206, 227, 348, 594
BmsI GCATC 3 cut(s) 14, 43, 388
BsaI GGTCTC 1 cut(s) 272
BsaJI CCNNGG 5 cut(s) 91, 128, 205, 225, 592
Bsc4I CCNNNNNNNGG 3 cut(s) 226, 488, 597
Bse1I ACTGG 2 cut(s) 434, 678
BseBI CCWGG 4 cut(s) 206, 227, 348, 594
BseDI CCNNGG 5 cut(s) 91, 128, 205, 225, 592
BseGI GGATG 3 cut(s) 403, 438, 442
BseLI CCNNNNNNNGG 3 cut(s) 226, 488, 597
BseMII CTCAG 2 cut(s) 537, 573
BseNI ACTGG 2 cut(s) 434, 678
BseRI GAGGAG 2 cut(s) 199, 381
BseXI GCAGC 1 cut(s) 628
BseYI CCCAGC 1 cut(s) 541
BsiHKAI GWGCWC 1 cut(s) 219
BslI CCNNNNNNNGG 3 cut(s) 226, 488, 597
BsmAI GTCTC 2 cut(s) 272, 363
BsmI GAATGC 1 cut(s) 674
Bso31I GGTCTC 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 1 cut(s) 552
Bsp19I CCATGG 1 cut(s) 91
BspCNI CTCAG 2 cut(s) 538, 572
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 3 cut(s) 89, 126, 611
BspTNI GGTCTC 1 cut(s) 272
BsrI ACTGG 2 cut(s) 434, 678
BssECI CCNNGG 5 cut(s) 91, 128, 205, 225, 592
BssMI GATC 1 cut(s) 552
BssNAI GTATAC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 91, 128
Bst1107I GTATAC 1 cut(s) 114
Bst2UI CCWGG 4 cut(s) 206, 227, 348, 594
Bst4CI ACNGT 1 cut(s) 35
BstAPI GCANNNNNTGC 1 cut(s) 641
BstDEI CTNAG 3 cut(s) 546, 559, 631
BstDSI CCRYGG 1 cut(s) 91
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 3 cut(s) 403, 438, 442
BstKTI GATC 1 cut(s) 555
BstMAI GTCTC 2 cut(s) 272, 363
BstMBI GATC 1 cut(s) 552
BstMWI GCNNNNNNNGC 1 cut(s) 641
BstNI CCWGG 4 cut(s) 206, 227, 348, 594
BstNSI RCATGY 3 cut(s) 335, 362, 376
BstSCI CCNGG 4 cut(s) 204, 225, 346, 592
BstV1I GCAGC 1 cut(s) 628
BstXI CCANNNNNNTGG 1 cut(s) 347
BstZ17I GTATAC 1 cut(s) 114
BtgI CCRYGG 1 cut(s) 91
BtsCI GGATG 3 cut(s) 403, 438, 442
BtsI GCAGTG 1 cut(s) 449
BtsIMutI CAGTG 1 cut(s) 449
CciI TCATGA 1 cut(s) 424
Cfr13I GGNCC 3 cut(s) 88, 124, 609
CviAII CATG 7 cut(s) 92, 290, 320, 332, 359, 373, 425
CviJI RGCY 7 cut(s) 17, 201, 250, 260, 454, 540, 545
CviKI_1 RGCY 7 cut(s) 17, 201, 250, 260, 454, 540, 545
DdeI CTNAG 3 cut(s) 546, 559, 631
DpnI GATC 1 cut(s) 554
DpnII GATC 1 cut(s) 552
Eco130I CCWWGG 2 cut(s) 91, 128
Eco31I GGTCTC 1 cut(s) 272
Eco32I GATATC 1 cut(s) 284
Eco47I GGWCC 3 cut(s) 88, 124, 609
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 609
EcoRI GAATTC 1 cut(s) 72
EcoRII CCWGG 4 cut(s) 204, 225, 346, 592
EcoRV GATATC 1 cut(s) 284
EcoT14I CCWWGG 2 cut(s) 91, 128
ErhI CCWWGG 2 cut(s) 91, 128
FaeI CATG 7 cut(s) 95, 293, 323, 335, 362, 376, 428
FatI CATG 7 cut(s) 91, 289, 319, 331, 358, 372, 424
FblI GTMKAC 1 cut(s) 113
Fnu4HI GCNGC 1 cut(s) 642
FokI GGATG 3 cut(s) 410, 445, 449
Fsp4HI GCNGC 1 cut(s) 642
FspBI CTAG 4 cut(s) 129, 251, 515, 537
GluI GCNGC 1 cut(s) 642
GsaI CCCAGC 1 cut(s) 545
Hin1II CATG 7 cut(s) 95, 293, 323, 335, 362, 376, 428
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 4 cut(s) 119, 190, 300, 478
HphI GGTGA 3 cut(s) 160, 214, 547
Hpy166II GTNNAC 2 cut(s) 114, 163
Hpy188I TCNGA 2 cut(s) 124, 562
Hpy188III TCNNGA 5 cut(s) 153, 194, 279, 425, 482
Hpy8I GTNNAC 2 cut(s) 114, 163
HpyAV CCTTC 2 cut(s) 59, 595
HpyCH4III ACNGT 1 cut(s) 35
HpyCH4V TGCA 3 cut(s) 56, 355, 447
HpyF10VI GCNNNNNNNGC 1 cut(s) 641
HpyF3I CTNAG 3 cut(s) 546, 559, 631
Hsp92II CATG 7 cut(s) 95, 293, 323, 335, 362, 376, 428
Kzo9I GATC 1 cut(s) 552
LmnI GCTCC 1 cut(s) 340
Lsp1109I GCAGC 1 cut(s) 628
LweI GCATC 3 cut(s) 14, 43, 388
MaeI CTAG 4 cut(s) 129, 251, 515, 537
MaeIII GTNAC 2 cut(s) 39, 467
MalI GATC 1 cut(s) 554
MboI GATC 1 cut(s) 552
MboII GAAGA 3 cut(s) 424, 562, 677
MfeI CAATTG 1 cut(s) 57
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 6 cut(s) 9, 57, 72, 148, 463, 620
MseI TTAA 5 cut(s) 81, 135, 147, 294, 660
MslI CAYNNNNRTG 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 545
MspR9I CCNGG 4 cut(s) 206, 227, 348, 594
MunI CAATTG 1 cut(s) 57
Mva1269I GAATGC 1 cut(s) 674
MvaI CCWGG 4 cut(s) 206, 227, 348, 594
MwoI GCNNNNNNNGC 1 cut(s) 641
NcoI CCATGG 1 cut(s) 91
NdeII GATC 1 cut(s) 552
NlaIII CATG 7 cut(s) 95, 293, 323, 335, 362, 376, 428
NlaIV GGNNCC 3 cut(s) 89, 126, 611
NmuCI GTSAC 1 cut(s) 39
NspI RCATGY 3 cut(s) 335, 362, 376
PagI TCATGA 1 cut(s) 424
PciI ACATGT 2 cut(s) 358, 372
PctI GAATGC 1 cut(s) 674
PfeI GAWTC 4 cut(s) 119, 190, 300, 478
PflMI CCANNNNNTGG 1 cut(s) 488
PkrI GCNGC 1 cut(s) 643
PpuMI RGGWCCY 1 cut(s) 609
PscI ACATGT 2 cut(s) 358, 372
PsiI TTATAA 1 cut(s) 326
Psp5II RGGWCCY 1 cut(s) 609
Psp6I CCWGG 4 cut(s) 204, 225, 346, 592
PspFI CCCAGC 1 cut(s) 541
PspGI CCWGG 4 cut(s) 204, 225, 346, 592
PspN4I GGNNCC 3 cut(s) 89, 126, 611
PspPI GGNCC 3 cut(s) 88, 124, 609
PspPPI RGGWCCY 1 cut(s) 609
PvuII CAGCTG 1 cut(s) 545
RseI CAYNNNNRTG 1 cut(s) 407
SaqAI TTAA 5 cut(s) 81, 135, 147, 294, 660
SatI GCNGC 1 cut(s) 642
Sau3AI GATC 1 cut(s) 552
Sau96I GGNCC 3 cut(s) 88, 124, 609
ScrFI CCNGG 4 cut(s) 206, 227, 348, 594
SduI GDGCHC 1 cut(s) 219
SetI ASST 8 cut(s) 134, 168, 203, 222, 231, 456, 547, 560
SfaNI GCATC 3 cut(s) 14, 43, 388
SinI GGWCC 3 cut(s) 88, 124, 609
SmiMI CAYNNNNRTG 1 cut(s) 407
SpeI ACTAGT 1 cut(s) 514
Sse9I AATT 6 cut(s) 9, 57, 72, 148, 463, 620
SspMI CTAG 4 cut(s) 129, 251, 515, 537
StyD4I CCNGG 4 cut(s) 204, 225, 346, 592
StyI CCWWGG 2 cut(s) 91, 128
TaaI ACNGT 1 cut(s) 35
TasI AATT 6 cut(s) 9, 57, 72, 148, 463, 620
TfiI GAWTC 4 cut(s) 119, 190, 300, 478
Tru1I TTAA 5 cut(s) 81, 135, 147, 294, 660
Tru9I TTAA 5 cut(s) 81, 135, 147, 294, 660
TscAI CASTG 1 cut(s) 449
TseFI GTSAC 1 cut(s) 39
TseI GCWGC 1 cut(s) 641
Tsp45I GTSAC 1 cut(s) 39
TspDTI ATGAA 5 cut(s) 44, 413, 425, 681, 691
TspGWI ACGGA 1 cut(s) 66
TspRI CASTG 1 cut(s) 449
Van91I CCANNNNNTGG 1 cut(s) 488
VpaK11BI GGWCC 3 cut(s) 88, 124, 609
XagI CCTNNNNNAGG 1 cut(s) 224
XapI RAATTY 2 cut(s) 72, 620
XceI RCATGY 3 cut(s) 335, 362, 376
XmaJI CCTAGG 1 cut(s) 128
XmiI GTMKAC 1 cut(s) 113
XspI CTAG 4 cut(s) 129, 251, 515, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.