MD10G1233100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000276
Physical Location & Seq
Forward (+)
32928166 .. 32929122
957 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1233100.v1.1.491

Sequence Viewer

Length: 321 bp
ATGCATGTACGTAATATTAGTATCTCTGGCAAATTTTTCCTTGGCTCTTGCAGATCGCCGACTATCGGTATAAGTCTGGGCAAAGACTCGAAGAAAATGAAGCGCAAGATTGTGATGAAGGTACCGATGAATTGCCGGAAATGCCAAACGAAGGCGCTCAAAATTGCTACTACAGTAGATGGTGTCAATTCTGTGGCGTTGGGAGAGGAAAAAGACAGAGTGGTGGTGATTGGGGAGGGAGTGGACGCCTTTAAGTTGGCCAAGAGCTTAAGGAAGAAATTCAAGGCCGCCGATATTATCACCGTCGCAGAAGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

107

Amino Acids

11.56

Weight (kDa)

10.17

Isoelectric Point (pI)

28.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 38 - 92 7.5e-06 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05030 AT4G05030
fragaria_vesca FvH4_3g24080 FvH4_3g24080 FvH4_3g24100 FvH4_3g24100 FvH4_3g42050 FvH4_3g42060 FvH4_3g42070 FvH4_3g42071 FvH4_3g42080 FvH4_3g42090 FvH4_3g42100 FvH4_3g42110
malus_domestica MD03G1038100.v1.1 MD03G1038400.v1.1 MD03G1038600.v1.1 MD03G1038900.v1.1 MD03G1039000.v1.1 MD03G1039100.v1.1 MD03G1188500.v1.1 MD10G1233100.v1.1 MD10G1233300.v1.1 MD10G1341200.v1.1 MD11G1039200.v1.1 MD11G1039300.v1.1 MD11G1204400.v1.1 MD11G1204600.v1.1
prunus_persica Prupe.2G123900_v2.0.a1 Prupe.4G001900_v2.0.a1 Prupe.4G234700_v2.0.a1 Prupe.4G234800_v2.0.a1 Prupe.6G025900_v2.0.a1 Prupe.6G030200_v2.0.a1 Prupe.6G030300_v2.0.a1 Prupe.6G030400_v2.0.a1 Prupe.6G030600_v2.0.a1 Prupe.6G030900_v2.0.a1 Prupe.6G031000_v2.0.a1 Prupe.6G031100_v2.0.a1 Prupe.6G031100_v2.0.a1
pyrus_communis pycom03g02940 pycom03g02950 pycom03g02980 pycom03g02990 pycom03g03010 pycom11g03310 pycom11g03320
rosa_chinensis RchiOBHm_Chr3g0484311 RchiOBHm_Chr5g0000231 RchiOBHm_Chr5g0042661 RchiOBHm_Chr5g0042671 RchiOBHm_Chr5g0075281 RchiOBHm_Chr5g0075291 RchiOBHm_Chr5g0075321 RchiOBHm_Chr5g0075331
rosa_laevigata RLG00000034149 RLG00000036545 RLG00000036546 RLG00000036547 RLG00000036548 RLG00000036549 RLG00000036550
rosa_multiflora Rmu_sc0000295.1_g000011 Rmu_sc0000770.1_g000041 Rmu_sc0000770.1_g000049 Rmu_sc0002564.1_g000002 Rmu_sc0002564.1_g000003 Rmu_sc0002564.1_g000016
rosa_roxburghii Rroxscaffold_1G00006200 Rroxscaffold_1G00006210 Rroxscaffold_1G00006220 Rroxscaffold_1G00006250 Rroxscaffold_1G00006270 Rroxscaffold_1G00037900 Rroxscaffold_1G00037980 Rroxscaffold_6G00397790
rosa_rugosa Rorug03G0210600 Rorug05G0203800 Rorug05G0203900 Rorug05G0438100 Rorug05G0438200 Rorug05G0438700 Rorug05G0438900 Rorug05G0438900
rosa_samantha Rh3AG259700 Rh3AG259800 Rh3BG296300 Rh3DG289900 Rh5AG001600 Rh5AG288900 Rh5AG289000 Rh5AG289200 Rh5AG494800 Rh5AG495000 Rh5AG495200 Rh5AG495300 Rh5BG293000 Rh5BG293100 Rh5BG293300 Rh5BG516400 Rh5BG516500 Rh5BG516900 Rh5BG517000 Rh5CG001900 Rh5CG325700 Rh5CG325900 Rh5CG326000 Rh5CG540200 Rh5CG540300 Rh5CG540500 Rh5CG540600 Rh5DG001900 Rh5DG302900 Rh5DG303000 Rh5DG303200 Rh5DG529100 Rh5DG529300 Rh5DG529400 Rh5DG532500
rosa_wichuraiana Rw3G023420 Rw3G023430 Rw5G000210 Rw5G026800 Rw5G045910 Rw5G045920 Rw5G045930 Rw5G045940 Rw5G045950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 121
AccB1I GGYRCC 1 cut(s) 121
AciI CCGC 1 cut(s) 288
AcoI YGGCCR 1 cut(s) 258
AcsI RAATTY 2 cut(s) 32, 278
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 2 cut(s) 9, 123
AfiI CCNNNNNNNGG 2 cut(s) 65, 151
AflII CTTAAG 1 cut(s) 268
AgsI TTSAA 1 cut(s) 283
AluBI AGCT 1 cut(s) 267
AluI AGCT 1 cut(s) 267
AoxI GGCC 2 cut(s) 258, 285
ApoI RAATTY 2 cut(s) 32, 278
Asp700I GAANNNNTTC 1 cut(s) 278
Asp718I GGTACC 1 cut(s) 121
AspLEI GCGC 2 cut(s) 105, 157
AsuHPI GGTGA 2 cut(s) 238, 292
BalI TGGCCA 1 cut(s) 260
BanI GGYRCC 1 cut(s) 121
BccI CCATC 1 cut(s) 173
BfmI CTRYAG 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 158
BfrI CTTAAG 1 cut(s) 268
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
BmiI GGNNCC 1 cut(s) 123
BsaAI YACGTR 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 246
BsaJI CCNNGG 1 cut(s) 40
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 151
BseDI CCNNGG 1 cut(s) 40
BseLI CCNNNNNNNGG 2 cut(s) 65, 151
BshFI GGCC 2 cut(s) 260, 287
BshNI GGYRCC 1 cut(s) 121
BsiSI CCGG 1 cut(s) 136
BslI CCNNNNNNNGG 2 cut(s) 65, 151
BsnI GGCC 2 cut(s) 260, 287
Bsp143I GATC 1 cut(s) 53
BspACI CCGC 1 cut(s) 288
BspANI GGCC 2 cut(s) 260, 287
BspLI GGNNCC 1 cut(s) 123
BspT107I GGYRCC 1 cut(s) 121
BspTI CTTAAG 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 1 cut(s) 53
BssNI GRCGYC 1 cut(s) 246
BssT1I CCWWGG 1 cut(s) 40
Bst4CI ACNGT 2 cut(s) 175, 304
BstACI GRCGYC 1 cut(s) 246
BstAFI CTTAAG 1 cut(s) 268
BstBAI YACGTR 1 cut(s) 11
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 2 cut(s) 105, 157
BstKTI GATC 1 cut(s) 56
BstMBI GATC 1 cut(s) 53
BstMWI GCNNNNNNNGC 1 cut(s) 141
BstNSI RCATGY 1 cut(s) 8
BstSFI CTRYAG 1 cut(s) 171
BstSNI TACGTA 1 cut(s) 11
BsuRI GGCC 2 cut(s) 260, 287
CfoI GCGC 2 cut(s) 105, 157
CseI GACGC 1 cut(s) 254
Csp6I GTAC 2 cut(s) 8, 122
CviAII CATG 1 cut(s) 5
CviJI RGCY 4 cut(s) 45, 260, 267, 287
CviKI_1 RGCY 4 cut(s) 45, 260, 267, 287
CviQI GTAC 2 cut(s) 8, 122
DpnI GATC 1 cut(s) 55
DpnII GATC 1 cut(s) 53
EaeI YGGCCR 1 cut(s) 258
Eco105I TACGTA 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 40
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 1 cut(s) 8
FaiI YATR 2 cut(s) 6, 71
FatI CATG 1 cut(s) 4
Fnu4HI GCNGC 1 cut(s) 288
Fsp4HI GCNGC 1 cut(s) 288
GlaI GCGC 2 cut(s) 104, 156
GluI GCNGC 1 cut(s) 288
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 2 cut(s) 260, 287
HapII CCGG 1 cut(s) 136
HgaI GACGC 1 cut(s) 254
HhaI GCGC 2 cut(s) 105, 157
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 1 cut(s) 8
Hin6I GCGC 2 cut(s) 103, 155
HinP1I GCGC 2 cut(s) 103, 155
HinfI GANTC 1 cut(s) 86
HpaII CCGG 1 cut(s) 136
HphI GGTGA 2 cut(s) 238, 292
Hpy166II GTNNAC 1 cut(s) 244
Hpy8I GTNNAC 1 cut(s) 244
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 2 cut(s) 112, 145
HpyCH4III ACNGT 2 cut(s) 175, 304
HpyCH4IV ACGT 1 cut(s) 10
HpyCH4V TGCA 2 cut(s) 4, 51
HpyF10VI GCNNNNNNNGC 1 cut(s) 141
HpySE526I ACGT 1 cut(s) 10
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 1 cut(s) 8
HspAI GCGC 2 cut(s) 103, 155
KpnI GGTACC 1 cut(s) 125
Kzo9I GATC 1 cut(s) 53
LpnPI CCDG 3 cut(s) 12, 62, 149
MaeII ACGT 1 cut(s) 10
MalI GATC 1 cut(s) 55
MboI GATC 1 cut(s) 53
MboII GAAGA 2 cut(s) 103, 286
MlsI TGGCCA 1 cut(s) 260
MluCI AATT 5 cut(s) 32, 130, 162, 187, 278
MluNI TGGCCA 1 cut(s) 260
MlyI GAGTC 1 cut(s) 80
MnlI CCTC 2 cut(s) 199, 229
Mox20I TGGCCA 1 cut(s) 260
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 278
MscI TGGCCA 1 cut(s) 260
MseI TTAA 2 cut(s) 252, 269
Msp20I TGGCCA 1 cut(s) 260
MspCI CTTAAG 1 cut(s) 268
MspI CCGG 1 cut(s) 136
MwoI GCNNNNNNNGC 1 cut(s) 141
NdeII GATC 1 cut(s) 53
NlaIII CATG 1 cut(s) 8
NlaIV GGNNCC 1 cut(s) 123
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PdmI GAANNNNTTC 1 cut(s) 278
PkrI GCNGC 1 cut(s) 289
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 11
PspN4I GGNNCC 1 cut(s) 123
RsaI GTAC 2 cut(s) 9, 123
RsaNI GTAC 2 cut(s) 8, 122
SaqAI TTAA 2 cut(s) 252, 269
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 1 cut(s) 53
SchI GAGTC 1 cut(s) 80
SetI ASST 3 cut(s) 13, 123, 269
SfcI CTRYAG 1 cut(s) 171
SmlI CTYRAG 1 cut(s) 268
SmoI CTYRAG 1 cut(s) 268
SnaBI TACGTA 1 cut(s) 11
Sse9I AATT 5 cut(s) 32, 130, 162, 187, 278
SsiI CCGC 1 cut(s) 288
SspI AATATT 1 cut(s) 16
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 2 cut(s) 175, 304
TaiI ACGT 1 cut(s) 13
TaqI TCGA 1 cut(s) 89
TasI AATT 5 cut(s) 32, 130, 162, 187, 278
TauI GCSGC 1 cut(s) 290
Tru1I TTAA 2 cut(s) 252, 269
Tru9I TTAA 2 cut(s) 252, 269
TspDTI ATGAA 3 cut(s) 113, 131, 143
Vha464I CTTAAG 1 cut(s) 268
XapI RAATTY 2 cut(s) 32, 278
XceI RCATGY 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 278
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.