MD10G1233300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000276
Physical Location & Seq
Forward (+)
32933296 .. 32934157
862 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1233300.v1.1.491

Sequence Viewer

Length: 252 bp
ATGTCTGGGCAAAAAGACTCGAAGAAAATGAAGCGTAAGATTGTGATGAAGGTACCGATGAATTGCCGGAAATGCCAAGCGAAGGCGCTCAAAATTGCTGCTACAGTAGATGGTGTGAGTTCTGTGGCGTTGGGAGAGGAAAAAGACAGGGTGGTGGTGACTGGGGAGGGAGTGGACGCCATTAAGTTGGCCAAGAGCTTACGGAAGAAATTCATGGCCGCCGACATTATCACCGTCGCAGAAGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

84

Amino Acids

8.98

Weight (kDa)

9.96

Isoelectric Point (pI)

18.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 15 - 69 2.1e-07 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05030 AT4G05030
fragaria_vesca FvH4_3g24080 FvH4_3g24080 FvH4_3g24100 FvH4_3g24100 FvH4_3g42050 FvH4_3g42060 FvH4_3g42070 FvH4_3g42071 FvH4_3g42080 FvH4_3g42090 FvH4_3g42100 FvH4_3g42110
malus_domestica MD03G1038100.v1.1 MD03G1038400.v1.1 MD03G1038600.v1.1 MD03G1038900.v1.1 MD03G1039000.v1.1 MD03G1039100.v1.1 MD03G1188500.v1.1 MD10G1233100.v1.1 MD10G1233300.v1.1 MD10G1341200.v1.1 MD11G1039200.v1.1 MD11G1039300.v1.1 MD11G1204400.v1.1 MD11G1204600.v1.1
prunus_persica Prupe.2G123900_v2.0.a1 Prupe.4G001900_v2.0.a1 Prupe.4G234700_v2.0.a1 Prupe.4G234800_v2.0.a1 Prupe.6G025900_v2.0.a1 Prupe.6G030200_v2.0.a1 Prupe.6G030300_v2.0.a1 Prupe.6G030400_v2.0.a1 Prupe.6G030600_v2.0.a1 Prupe.6G030900_v2.0.a1 Prupe.6G031000_v2.0.a1 Prupe.6G031100_v2.0.a1 Prupe.6G031100_v2.0.a1
pyrus_communis pycom03g02940 pycom03g02950 pycom03g02980 pycom03g02990 pycom03g03010 pycom11g03310 pycom11g03320
rosa_chinensis RchiOBHm_Chr3g0484311 RchiOBHm_Chr5g0000231 RchiOBHm_Chr5g0042661 RchiOBHm_Chr5g0042671 RchiOBHm_Chr5g0075281 RchiOBHm_Chr5g0075291 RchiOBHm_Chr5g0075321 RchiOBHm_Chr5g0075331
rosa_laevigata RLG00000034149 RLG00000036545 RLG00000036546 RLG00000036547 RLG00000036548 RLG00000036549 RLG00000036550
rosa_multiflora Rmu_sc0000295.1_g000011 Rmu_sc0000770.1_g000041 Rmu_sc0000770.1_g000049 Rmu_sc0002564.1_g000002 Rmu_sc0002564.1_g000003 Rmu_sc0002564.1_g000016
rosa_roxburghii Rroxscaffold_1G00006200 Rroxscaffold_1G00006210 Rroxscaffold_1G00006220 Rroxscaffold_1G00006250 Rroxscaffold_1G00006270 Rroxscaffold_1G00037900 Rroxscaffold_1G00037980 Rroxscaffold_6G00397790
rosa_rugosa Rorug03G0210600 Rorug05G0203800 Rorug05G0203900 Rorug05G0438100 Rorug05G0438200 Rorug05G0438700 Rorug05G0438900 Rorug05G0438900
rosa_samantha Rh3AG259700 Rh3AG259800 Rh3BG296300 Rh3DG289900 Rh5AG001600 Rh5AG288900 Rh5AG289000 Rh5AG289200 Rh5AG494800 Rh5AG495000 Rh5AG495200 Rh5AG495300 Rh5BG293000 Rh5BG293100 Rh5BG293300 Rh5BG516400 Rh5BG516500 Rh5BG516900 Rh5BG517000 Rh5CG001900 Rh5CG325700 Rh5CG325900 Rh5CG326000 Rh5CG540200 Rh5CG540300 Rh5CG540500 Rh5CG540600 Rh5DG001900 Rh5DG302900 Rh5DG303000 Rh5DG303200 Rh5DG529100 Rh5DG529300 Rh5DG529400 Rh5DG532500
rosa_wichuraiana Rw3G023420 Rw3G023430 Rw5G000210 Rw5G026800 Rw5G045910 Rw5G045920 Rw5G045930 Rw5G045940 Rw5G045950

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 52
AccB1I GGYRCC 1 cut(s) 52
AciI CCGC 1 cut(s) 219
AcoI YGGCCR 2 cut(s) 189, 216
AcsI RAATTY 1 cut(s) 209
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 1 cut(s) 54
AfiI CCNNNNNNNGG 1 cut(s) 82
AluBI AGCT 1 cut(s) 198
AluI AGCT 1 cut(s) 198
AoxI GGCC 2 cut(s) 189, 216
ApeKI GCWGC 1 cut(s) 98
ApoI RAATTY 1 cut(s) 209
Asp700I GAANNNNTTC 1 cut(s) 209
Asp718I GGTACC 1 cut(s) 52
AspLEI GCGC 1 cut(s) 88
AsuHPI GGTGA 2 cut(s) 169, 223
BalI TGGCCA 1 cut(s) 191
BanI GGYRCC 1 cut(s) 52
BbvI GCAGC 1 cut(s) 85
BccI CCATC 1 cut(s) 104
BfmI CTRYAG 1 cut(s) 102
BfoI RGCGCY 1 cut(s) 89
BisI GCNGC 2 cut(s) 99, 219
BlsI GCNGC 2 cut(s) 100, 220
BmiI GGNNCC 1 cut(s) 54
BmrI ACTGGG 1 cut(s) 171
BmuI ACTGGG 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 177
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse1I ACTGG 1 cut(s) 166
BseLI CCNNNNNNNGG 1 cut(s) 82
BseNI ACTGG 1 cut(s) 166
BseXI GCAGC 1 cut(s) 85
BshFI GGCC 2 cut(s) 191, 218
BshNI GGYRCC 1 cut(s) 52
BsiSI CCGG 1 cut(s) 67
BslI CCNNNNNNNGG 1 cut(s) 82
BsnI GGCC 2 cut(s) 191, 218
BspACI CCGC 1 cut(s) 219
BspANI GGCC 2 cut(s) 191, 218
BspLI GGNNCC 1 cut(s) 54
BspT107I GGYRCC 1 cut(s) 52
BsrI ACTGG 1 cut(s) 166
BssNI GRCGYC 1 cut(s) 177
Bst4CI ACNGT 2 cut(s) 106, 235
BstACI GRCGYC 1 cut(s) 177
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 1 cut(s) 88
BstMWI GCNNNNNNNGC 1 cut(s) 72
BstSFI CTRYAG 1 cut(s) 102
BstV1I GCAGC 1 cut(s) 85
BstXI CCANNNNNNTGG 1 cut(s) 187
BsuRI GGCC 2 cut(s) 191, 218
CfoI GCGC 1 cut(s) 88
CseI GACGC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 53
CviAII CATG 1 cut(s) 214
CviJI RGCY 3 cut(s) 191, 198, 218
CviKI_1 RGCY 3 cut(s) 191, 198, 218
CviQI GTAC 1 cut(s) 53
EaeI YGGCCR 2 cut(s) 189, 216
FaeI CATG 1 cut(s) 217
FaiI YATR 1 cut(s) 215
FatI CATG 1 cut(s) 213
Fnu4HI GCNGC 2 cut(s) 99, 219
Fsp4HI GCNGC 2 cut(s) 99, 219
GlaI GCGC 1 cut(s) 87
GluI GCNGC 2 cut(s) 99, 219
HaeII RGCGCY 1 cut(s) 89
HaeIII GGCC 2 cut(s) 191, 218
HapII CCGG 1 cut(s) 67
HgaI GACGC 1 cut(s) 185
HhaI GCGC 1 cut(s) 88
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 1 cut(s) 217
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HinfI GANTC 1 cut(s) 17
HpaII CCGG 1 cut(s) 67
HphI GGTGA 2 cut(s) 169, 223
Hpy166II GTNNAC 1 cut(s) 175
Hpy8I GTNNAC 1 cut(s) 175
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 2 cut(s) 43, 76
HpyCH4III ACNGT 2 cut(s) 106, 235
HpyF10VI GCNNNNNNNGC 1 cut(s) 72
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 1 cut(s) 217
HspAI GCGC 1 cut(s) 86
KpnI GGTACC 1 cut(s) 56
LpnPI CCDG 3 cut(s) 80, 133, 147
Lsp1109I GCAGC 1 cut(s) 85
MaeIII GTNAC 1 cut(s) 157
MboII GAAGA 2 cut(s) 34, 217
MlsI TGGCCA 1 cut(s) 191
MluCI AATT 3 cut(s) 61, 93, 209
MluNI TGGCCA 1 cut(s) 191
MlyI GAGTC 1 cut(s) 11
MnlI CCTC 2 cut(s) 130, 160
Mox20I TGGCCA 1 cut(s) 191
MroXI GAANNNNTTC 1 cut(s) 209
MscI TGGCCA 1 cut(s) 191
MseI TTAA 1 cut(s) 183
Msp20I TGGCCA 1 cut(s) 191
MspI CCGG 1 cut(s) 67
MwoI GCNNNNNNNGC 1 cut(s) 72
NlaIII CATG 1 cut(s) 217
NlaIV GGNNCC 1 cut(s) 54
NmuCI GTSAC 1 cut(s) 157
PdmI GAANNNNTTC 1 cut(s) 209
PkrI GCNGC 2 cut(s) 100, 220
PleI GAGTC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 11
PspN4I GGNNCC 1 cut(s) 54
RsaI GTAC 1 cut(s) 54
RsaNI GTAC 1 cut(s) 53
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 2 cut(s) 99, 219
SchI GAGTC 1 cut(s) 11
SetI ASST 2 cut(s) 54, 200
SfcI CTRYAG 1 cut(s) 102
SgeI CNNG 8 cut(s) 18, 31, 79, 89, 160, 174, 205, 226
Sse9I AATT 3 cut(s) 61, 93, 209
SsiI CCGC 1 cut(s) 219
TaaI ACNGT 2 cut(s) 106, 235
TaqI TCGA 1 cut(s) 20
TasI AATT 3 cut(s) 61, 93, 209
TauI GCSGC 1 cut(s) 221
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 1 cut(s) 98
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 4 cut(s) 44, 62, 74, 202
TspGWI ACGGA 1 cut(s) 217
XapI RAATTY 1 cut(s) 209
XmnI GAANNNNTTC 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.