MD10G1333400.v1.1

gag-polypeptide of LTR copia-type

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000562
Physical Location & Seq
Forward (+)
41064502 .. 41067561
3060 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1333400.v1.1.491

Sequence Viewer

Length: 2637 bp
ATGTCTGATCCTATCAATTGGTACCAGAGCCAGAGATCCGCAATGGATCTATGGTTTGGACCCGTCGATCCCACACTTCCAGCGCCGTGGACAAGTGTGACGGATGATTCCAGTGGTATGGTGTTTTATTGGAATCCAGAAACCAATGTTTCTCAGTATGAATATCCAAACCCGCCTCCACCACCTCCTCCTCCTCCCGATCACTACCAGTTTCCATTCAAGTATACACCTACCTTTCCCTCATCTGCTCTTACCTATCAACCACCAGATTACCCAGCCTCGTATATGCCTGAGCTTCCCGTTGCTCCTGTATATACCAGTCCCATTTTCCTCCCGCACCCCGCACCACCTTCCGTTGTGTTTTCGGCCTCCATCGATGTCCCTCAAATCCCTCATTCCTATACCACAAACCCTAATTTTCAAGATCCCCAAATTTACTCTCCATCGTCGTACGAACAAAATTCGTGGGTTTTCGATTCTGAGAACCGTCTACGATTTAATGACCCCCATCAGGTATCTCCTTACGAAATTGACTTGGGTCCCGATTTGAACGTGCATACAGCTTCTACAGCAGGAAATGTCTTCAGCATGGTCAGAGCAGTGATCCCTCAAGGACCTGCATCTGTGTTCTTCCGAAATCCTACCAAATTCAAACCAGTTAAAGCCATTATGGCAACATCATCAGAGAAAGTTTCAAAAAACGAAACTATGGATACCAACCCAATTCAACGTTTGAGCTCAGTGCTCCTAAACGAATTCAACTATCTGCCTTGGTCACGAGCTATTTCTCTCGCTCTTGGAGGACGTGGAAAGCTCCAGTTCATTCAAAAGGATGATATCATGCCCGAAACAACTTCACAAGATTATGCAGCATGGGTGTCCCAAGATCAACTTATCATGTCTTGGCTGTTGAATTCAATGGAACCAAAGATGGCTGAGATTTTCAGTTATTCAGTATCATCTCACCACCTTTGGATTTCTGTACGAGACATGTATGGAAATCTAAACAATGCTGCGCGAGTCTTCCAGCTGAAGAAAGATCTTACAGAATTACAACAGGGAAATCTCTCTTTTGTTCAACATCTTGGCAACTTGAAGGCCAAATGGAATGAATTGGATCTCTACAGACCTCATACCACGGACACAACCATTCTTTTAAAGCGTGCTGAAGAAGACAAAGTGTTCCAGTTGCTTGCCAGCATCGGACCAGAATATGAGGATCTTAAAAGTCATCTCCTGATGACACCTGAACTTCCCACCTTCCAGATGGTCTGCAACGTTATTCAACGCGAAGAAATAAGGAAGAAGGTCATGAATGCAGTCGTTGTCGTTGGAGAATCAGATCTTAGGCCATCAGAAGCAAGAGCCTTCACCTCTTTCAGACCATACAAGGGAAAACGACCAGACTTAAAGTGTTCTCACTGTGTGAAAATAGGTCGTCCAGGCATTGGACACATAAAAGAAAAGTGCTGGATTCTTCATCCAGAACTTAAACCCAAGTTCAATGATGATGGAAGAGCACCAAGAAACCAAATGAAGCCTGCCTACACTCATCAAGGAAACCTTGTTTGCGACAATGTCTCCAAAAATGTGATGAACTCTTCTTCTAGTCCCATTACTCTCATAAATGAATTTGCTAACTTCCTGCAACAGAAACAAGGGACAACAAATCATGAAAATCCTGCAACCATGCTCGGCAAGTTTGCTGGTTTCCTCGAACAATCAAGCTCAGTGTCACAAAATGATGTGCCAGGTATTGTTGCAACTATTTCCACTGCATTGGATCTTAGTAGTAGCCGTGGTTTTTGGATAGTCGACTCAGGAGCTTCTGATCACATGACTAACGATGCATCTTTACTAAATGATTTTCAAAACTTCTCCACTCCTTCTTTTATCTCCGTAGCTAATGGCACCAAAGTTAGTATAGTAGGCATAGGAAAACTCAAAATCTTCTCAAGTAAAAAGGAATCCCTTGTCATGCATGTCCCTTCCTTTCCTTTCAAGTTGCTGTTAGTAGGAAGTATAACACAATTGCTAAATTGTCTTGCAATTTTTTCCACTCACTGTGTGATTTTCCAGGACCGAGTTTCGAAGGAAACGATTGGTAAAGGTTTCTTCCTCAATGGACTGTACTATGTCAATCTCTCTTCAAGTTTTCCAAAAGGTTTCTACGTAAACTCAAACACAGTAAACAAGGAGCAGCTGTGGCACATGAGATTAGCTCATCCTTCTTCCCATGTTATGTCTTTTATTTTTCCTAGTTTCTGTAAAGAGATTTCAGATTGTGAAGTTTGTCAAAAATCTAAGTCCACCAGGTTACCCTTTCCCCATTCTCTCTCTAGAGCTACCAAAGTGTTTGAAATTGTCCATTCCGACATTTGGGGACCTGCAACCTTAAAGTCTTTTGATGGCTACAGATTCTTCATCTCTTTCATTGATGATTTCTCTAGAACTACCTTTGTGTATTTACTTAAATTCAAGCACGAAGCTTTCCAGTGTTTCAAAAATTTTCATAACTTAGTTCAAAATCATTTTAACTCTAATATTGGCATCTTAAGGTCTGATAATGGCTCTGAGTATACTTCCAAAATCATGACAACTTACTTAAGTGAGCATGGAATCTTGCAAACAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01110 map00940 map01110
Pfam Domains
Protein Families
Retrotran_gag_2

Protein Analysis

879

Amino Acids

99.15

Weight (kDa)

7.14

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WW PF00397 26 - 56 6.5e-08 WW domain
Retrotran_gag_3 PF14244 245 - 276 2.6e-07 gag-polypeptide of LTR copia-type
Retrotran_gag_2 PF14223 274 - 437 4.7e-10 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 603 - 676 6e-14 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 709 - 770 1.3e-08 GAG-pre-integrase domain
rve PF00665 784 - 876 2.4e-09 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000562)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17331 FvH4_3g36541
malus_domestica MD07G1078600.v1.1 MD10G1237700.v1.1 MD10G1333400.v1.1 MD11G1102200.v1.1 MD13G1138400.v1.1 MD14G1033400.v1.1 MD15G1053300.v1.1
prunus_persica Prupe.1G465400_v2.0.a1 Prupe.3G136000_v2.0.a1 Prupe.7G119900_v2.0.a1
pyrus_communis pycom01g04630 pycom03g13050 pycom04g21190 pycom05g04910 pycom05g05510 pycom08g15850 pycom09g15040 pycom1094g00010 pycom11g02000 pycom12g06150 pycom13g24110 pycom13g24910 pycom14g00050 pycom15g21970 pycom15g35170 pycom15g35180 pycom16g14620 pycom16g14630 pycom808g00110
rosa_chinensis RchiOBHm_Chr1g0322851 RchiOBHm_Chr1g0365941 RchiOBHm_Chr3g0453341 RchiOBHm_Chr4g0388371 RchiOBHm_Chr4g0394021 RchiOBHm_Chr5g0013181 RchiOBHm_Chr6g0261721 RchiOBHm_Chr6g0261731 RchiOBHm_Chr7g0188861 RchiOBHm_Chr7g0192331 RchiOBHm_Chr7g0231721
rosa_multiflora Rmu_sc0002521.1_g000006 Rmu_sc0003255.1_g000026 Rmu_sc0004191.1_g000036 Rmu_sc0004414.1_g000014 Rmu_sc0005670.1_g000006 Rmu_sc0006081.1_g000001 Rmu_sc0007215.1_g000004 Rmu_sc0010088.1_g000010 Rmu_sc0020285.1_g000003 Rmu_sc0026896.1_g000002 Rmu_ssc0000019.1_g000029
rosa_roxburghii Rroxscaffold_3G00224890 Rroxscaffold_6G00400640 Rroxscaffold_7G00176590 Rroxscaffold_7G00189230
rosa_samantha Rh4BG046700
rosa_wichuraiana Rw1G012690 Rw1G031370 Rw2G042180 Rw2G043670 Rw2G046240 Rw2G049890 Rw2G050760 Rw2G051280 Rw2G053590 Rw3G007590 Rw3G026850 Rw4G001540 Rw4G009760 Rw4G019270 Rw5G000720 Rw5G005620 Rw5G041560 Rw6G007370 Rw6G009830 Rw6G015360 Rw6G031160 Rw7G004930 Rw7G011010 Rw7G024230 Rw7G028240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 625, 2395
Acc65I GGTACC 1 cut(s) 21
AccB1I GGYRCC 2 cut(s) 21, 1910
AccB7I CCANNNNNTGG 1 cut(s) 1448
AccI GTMKAC 4 cut(s) 224, 490, 1815, 2579
AccII CGCG 2 cut(s) 1018, 1290
AciI CCGC 4 cut(s) 39, 173, 335, 342
AclI AACGTT 2 cut(s) 730, 1278
AclWI GGATC 9 cut(s) 2, 30, 54, 62, 419, 598, 1125, 1227, 1791
AcsI RAATTY 8 cut(s) 432, 460, 647, 755, 913, 1631, 2474, 2506
AcuI CTGAAG 3 cut(s) 568, 1052, 1188
AdeI CACNNNGTG 2 cut(s) 1426, 2068
AfaI GTAC 4 cut(s) 23, 452, 984, 2132
AfiI CCNNNNNNNGG 4 cut(s) 511, 1391, 1448, 2379
AflII CTTAAG 2 cut(s) 2554, 2605
AflIII ACRYGT 1 cut(s) 990
AjiI CACGTC 1 cut(s) 806
AjnI CCWGG 4 cut(s) 1441, 1750, 2076, 2312
Alw21I GWGCWC 3 cut(s) 740, 747, 1522
Alw26I GTCTC 2 cut(s) 981, 1585
AlwI GGATC 9 cut(s) 2, 30, 54, 62, 419, 598, 1125, 1227, 1791
AoxI GGCC 3 cut(s) 366, 1098, 1349
ApeKI GCWGC 3 cut(s) 869, 1013, 2200
ApoI RAATTY 8 cut(s) 432, 460, 647, 755, 913, 1631, 2474, 2506
Asp718I GGTACC 1 cut(s) 21
AspLEI GCGC 2 cut(s) 85, 1018
AspS9I GGNCC 6 cut(s) 59, 539, 614, 1205, 2080, 2384
AsuHPI GGTGA 2 cut(s) 956, 1363
AsuII TTCGAA 1 cut(s) 2090
AvaII GGWCC 6 cut(s) 59, 539, 614, 1205, 2080, 2384
BanI GGYRCC 2 cut(s) 21, 1910
BanII GRGCYC 1 cut(s) 740
BarI GAAGNNNNNNTAC 2 cut(s) 1529, 1561
BauI CACGAG 1 cut(s) 777
BbsI GAAGAC 3 cut(s) 574, 1015, 1179
Bbv12I GWGCWC 3 cut(s) 740, 747, 1522
BbvI GCAGC 3 cut(s) 881, 1000, 2212
BccI CCATC 8 cut(s) 380, 451, 516, 925, 1261, 1360, 1505, 2402
BceAI ACGGC 2 cut(s) 70, 1782
BciT130I CCWGG 4 cut(s) 1443, 1752, 2078, 2314
BciVI GTATCC 1 cut(s) 706
BclI TGATCA 1 cut(s) 1831
BcoDI GTCTC 2 cut(s) 981, 1585
BfaI CTAG 5 cut(s) 1608, 2259, 2340, 2448, 2635
BfmI CTRYAG 3 cut(s) 567, 1123, 2413
BfoI RGCGCY 1 cut(s) 86
BfrI CTTAAG 2 cut(s) 2554, 2605
BfuAI ACCTGC 2 cut(s) 625, 2395
BfuI GTATCC 1 cut(s) 706
BglI GCCNNNNNGGC 1 cut(s) 671
BglII AGATCT 2 cut(s) 1039, 1342
BisI GCNGC 3 cut(s) 870, 1014, 2201
BlsI GCNGC 3 cut(s) 871, 1015, 2202
Bme1390I CCNGG 4 cut(s) 1443, 1752, 2078, 2314
Bme18I GGWCC 6 cut(s) 59, 539, 614, 1205, 2080, 2384
BmgBI CACGTC 1 cut(s) 806
BmgT120I GGNCC 6 cut(s) 59, 539, 614, 1205, 2080, 2384
BmiI GGNNCC 7 cut(s) 23, 61, 540, 541, 924, 1912, 2385
BmrFI CCNGG 4 cut(s) 1443, 1752, 2078, 2314
BmsI GCATC 5 cut(s) 629, 1209, 1837, 1859, 2559
BoxI GACNNNNGTC 1 cut(s) 537
BpiI GAAGAC 3 cut(s) 574, 1015, 1179
BplI GAGNNNNNCTC 2 cut(s) 2206, 2238
BpmI CTGGAG 1 cut(s) 800
Bpu10I CCTNAGC 1 cut(s) 291
Bpu14I TTCGAA 1 cut(s) 2090
BpuEI CTTGAG 2 cut(s) 594, 1939
Bsa29I ATCGAT 1 cut(s) 375
BsaAI YACGTR 1 cut(s) 2173
BsaJI CCNNGG 4 cut(s) 86, 770, 1137, 1798
BsaXI ACNNNNNCTCC 4 cut(s) 1565, 1595, 2189, 2219
Bsc4I CCNNNNNNNGG 4 cut(s) 511, 1391, 1448, 2379
Bse1I ACTGG 7 cut(s) 111, 208, 318, 656, 817, 1186, 2494
Bse3DI GCAATG 1 cut(s) 48
BseBI CCWGG 4 cut(s) 1443, 1752, 2078, 2314
BseCI ATCGAT 1 cut(s) 375
BseDI CCNNGG 4 cut(s) 86, 770, 1137, 1798
BseGI GGATG 4 cut(s) 109, 838, 1480, 2224
BseLI CCNNNNNNNGG 4 cut(s) 511, 1391, 1448, 2379
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 8 cut(s) 167, 282, 471, 753, 927, 1743, 1833, 2565
BseNI ACTGG 7 cut(s) 111, 208, 318, 656, 817, 1186, 2494
BseRI GAGGAG 3 cut(s) 177, 180, 183
BseXI GCAGC 3 cut(s) 881, 1000, 2212
BseYI CCCAGC 1 cut(s) 274
Bsh1236I CGCG 2 cut(s) 1018, 1290
BshFI GGCC 3 cut(s) 368, 1100, 1351
BshNI GGYRCC 2 cut(s) 21, 1910
BshVI ATCGAT 1 cut(s) 375
BsiHKAI GWGCWC 3 cut(s) 740, 747, 1522
BsiWI CGTACG 1 cut(s) 450
BslFI GGGAC 8 cut(s) 306, 365, 525, 865, 1596, 1675, 1970, 2397
BslI CCNNNNNNNGG 4 cut(s) 511, 1391, 1448, 2379
BsmAI GTCTC 2 cut(s) 981, 1585
BsmFI GGGAC 8 cut(s) 306, 365, 525, 865, 1596, 1675, 1970, 2397
BsmI GAATGC 1 cut(s) 1321
BsnI GGCC 3 cut(s) 368, 1100, 1351
Bsp119I TTCGAA 1 cut(s) 2090
Bsp1286I GDGCHC 3 cut(s) 740, 747, 1522
BspACI CCGC 4 cut(s) 39, 173, 335, 342
BspANI GGCC 3 cut(s) 368, 1100, 1351
BspCNI CTCAG 8 cut(s) 166, 283, 472, 752, 928, 1742, 1832, 2566
BspDI ATCGAT 1 cut(s) 375
BspFNI CGCG 2 cut(s) 1018, 1290
BspHI TCATGA 3 cut(s) 1311, 1672, 2592
BspLI GGNNCC 7 cut(s) 23, 61, 540, 541, 924, 1912, 2385
BspMI ACCTGC 2 cut(s) 625, 2395
BspPI GGATC 9 cut(s) 2, 30, 54, 62, 419, 598, 1125, 1227, 1791
BspQI GCTCTTC 1 cut(s) 1510
BspT104I TTCGAA 1 cut(s) 2090
BspT107I GGYRCC 2 cut(s) 21, 1910
BspTI CTTAAG 2 cut(s) 2554, 2605
BsrDI GCAATG 1 cut(s) 48
BsrI ACTGG 7 cut(s) 111, 208, 318, 656, 817, 1186, 2494
BssECI CCNNGG 4 cut(s) 86, 770, 1137, 1798
BssNAI GTATAC 2 cut(s) 225, 2580
BssSI CACGAG 1 cut(s) 777
BssT1I CCWWGG 1 cut(s) 770
Bst1107I GTATAC 2 cut(s) 225, 2580
Bst2BI CACGAG 1 cut(s) 777
Bst2UI CCWGG 4 cut(s) 1443, 1752, 2078, 2314
Bst4CI ACNGT 5 cut(s) 488, 1424, 2066, 2130, 2188
Bst6I CTCTTC 3 cut(s) 1510, 1606, 2152
BstAFI CTTAAG 2 cut(s) 2554, 2605
BstBAI YACGTR 1 cut(s) 2173
BstBI TTCGAA 1 cut(s) 2090
BstC8I GCNNGC 4 cut(s) 1164, 1194, 1198, 1542
BstDSI CCRYGG 3 cut(s) 86, 1137, 1798
BstEII GGTNACC 1 cut(s) 2316
BstF5I GGATG 4 cut(s) 109, 838, 1480, 2224
BstFNI CGCG 2 cut(s) 1018, 1290
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 2 cut(s) 85, 1018
BstMAI GTCTC 2 cut(s) 981, 1585
BstMWI GCNNNNNNNGC 3 cut(s) 569, 671, 2206
BstNI CCWGG 4 cut(s) 1443, 1752, 2078, 2314
BstNSI RCATGY 2 cut(s) 994, 1985
BstPAI GACNNNNGTC 1 cut(s) 537
BstPI GGTNACC 1 cut(s) 2316
BstSCI CCNGG 4 cut(s) 1441, 1750, 2076, 2312
BstSFI CTRYAG 3 cut(s) 567, 1123, 2413
BstSNI TACGTA 1 cut(s) 2173
BstUI CGCG 2 cut(s) 1018, 1290
BstV1I GCAGC 3 cut(s) 881, 1000, 2212
BstV2I GAAGAC 3 cut(s) 574, 1015, 1179
BstX2I RGATCY 8 cut(s) 35, 46, 424, 1039, 1117, 1219, 1342, 1783
BstXI CCANNNNNNTGG 3 cut(s) 87, 118, 1780
BstYI RGATCY 8 cut(s) 35, 46, 424, 1039, 1117, 1219, 1342, 1783
BstZ17I GTATAC 2 cut(s) 225, 2580
Bsu15I ATCGAT 1 cut(s) 375
BsuI GTATCC 1 cut(s) 706
BsuRI GGCC 3 cut(s) 368, 1100, 1351
BsuTUI ATCGAT 1 cut(s) 375
BtgI CCRYGG 3 cut(s) 86, 1137, 1798
BtrI CACGTC 1 cut(s) 806
BtsCI GGATG 4 cut(s) 109, 838, 1480, 2224
BtsI GCAGTG 2 cut(s) 606, 1773
BtsIMutI CAGTG 8 cut(s) 118, 606, 747, 1420, 1737, 1773, 2062, 2501
BveI ACCTGC 2 cut(s) 625, 2395
Cac8I GCNNGC 4 cut(s) 1164, 1194, 1198, 1542
CciI TCATGA 3 cut(s) 1311, 1672, 2592
CfoI GCGC 2 cut(s) 85, 1018
Cfr13I GGNCC 6 cut(s) 59, 539, 614, 1205, 2080, 2384
ClaI ATCGAT 1 cut(s) 375
CsiI ACCWGGT 1 cut(s) 2312
Csp6I GTAC 4 cut(s) 22, 451, 983, 2131
CspCI CAANNNNNGTGG 2 cut(s) 446, 481
CviQI GTAC 4 cut(s) 22, 451, 983, 2131
DraI TTTAAA 1 cut(s) 1158
DraIII CACNNNGTG 2 cut(s) 1426, 2068
Eam1104I CTCTTC 3 cut(s) 1510, 1606, 2152
EarI CTCTTC 3 cut(s) 1510, 1606, 2152
Ecl136II GAGCTC 1 cut(s) 738
Eco105I TACGTA 1 cut(s) 2173
Eco130I CCWWGG 1 cut(s) 770
Eco24I GRGCYC 1 cut(s) 740
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 6 cut(s) 59, 539, 614, 1205, 2080, 2384
Eco53kI GAGCTC 1 cut(s) 738
Eco57I CTGAAG 3 cut(s) 568, 1052, 1188
Eco91I GGTNACC 1 cut(s) 2316
EcoICRI GAGCTC 1 cut(s) 738
EcoO109I RGGNCCY 3 cut(s) 539, 614, 2384
EcoO65I GGTNACC 1 cut(s) 2316
EcoRI GAATTC 2 cut(s) 755, 913
EcoRII CCWGG 4 cut(s) 1441, 1750, 2076, 2312
EcoRV GATATC 1 cut(s) 838
EcoT14I CCWWGG 1 cut(s) 770
EcoT22I ATGCAT 2 cut(s) 1852, 1983
EcoT38I GRGCYC 1 cut(s) 740
ErhI CCWWGG 1 cut(s) 770
FalI AAGNNNNNCTT 4 cut(s) 876, 908, 1548, 1580
FaqI GGGAC 8 cut(s) 306, 365, 525, 865, 1596, 1675, 1970, 2397
FauI CCCGC 3 cut(s) 180, 342, 349
FbaI TGATCA 1 cut(s) 1831
FblI GTMKAC 4 cut(s) 224, 490, 1815, 2579
Fnu4HI GCNGC 3 cut(s) 870, 1014, 2201
FokI GGATG 4 cut(s) 116, 845, 1467, 2211
FriOI GRGCYC 1 cut(s) 740
Fsp4HI GCNGC 3 cut(s) 870, 1014, 2201
FspBI CTAG 5 cut(s) 1608, 2259, 2340, 2448, 2635
GlaI GCGC 2 cut(s) 84, 1017
GluI GCNGC 3 cut(s) 870, 1014, 2201
GsaI CCCAGC 1 cut(s) 278
GsuI CTGGAG 1 cut(s) 800
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 3 cut(s) 368, 1100, 1351
HhaI GCGC 2 cut(s) 85, 1018
Hin6I GCGC 2 cut(s) 83, 1016
HinP1I GCGC 2 cut(s) 83, 1016
HincII GTYRAC 1 cut(s) 1816
HindII GTYRAC 1 cut(s) 1816
HindIII AAGCTT 1 cut(s) 2487
HphI GGTGA 2 cut(s) 956, 1363
Hpy166II GTNNAC 8 cut(s) 90, 225, 491, 1816, 2176, 2191, 2310, 2580
Hpy8I GTNNAC 8 cut(s) 90, 225, 491, 1816, 2176, 2191, 2310, 2580
Hpy99I CGWCG 2 cut(s) 68, 451
HpyAV CCTTC 9 cut(s) 360, 1090, 1270, 1300, 1378, 1896, 1998, 2086, 2238
HpyCH4III ACNGT 5 cut(s) 488, 1424, 2066, 2130, 2188
HpyCH4IV ACGT 5 cut(s) 552, 730, 805, 1278, 2172
HpyF10VI GCNNNNNNNGC 3 cut(s) 569, 671, 2206
HpySE526I ACGT 5 cut(s) 552, 730, 805, 1278, 2172
HspAI GCGC 2 cut(s) 83, 1016
KflI GGGWCCC 1 cut(s) 539
KpnI GGTACC 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 1831
LguI GCTCTTC 1 cut(s) 1510
LmnI GCTCC 5 cut(s) 310, 750, 819, 1823, 2197
Lsp1109I GCAGC 3 cut(s) 881, 1000, 2212
LweI GCATC 5 cut(s) 629, 1209, 1837, 1859, 2559
MabI ACCWGGT 1 cut(s) 2312
MaeI CTAG 5 cut(s) 1608, 2259, 2340, 2448, 2635
MaeII ACGT 5 cut(s) 552, 730, 805, 1278, 2172
MaeIII GTNAC 4 cut(s) 97, 774, 1734, 2316
MfeI CAATTG 2 cut(s) 16, 2030
MflI RGATCY 8 cut(s) 35, 46, 424, 1039, 1117, 1219, 1342, 1783
MhlI GDGCHC 3 cut(s) 740, 747, 1522
MlyI GAGTC 2 cut(s) 1029, 1811
MmeI TCCRAC 2 cut(s) 1312, 2397
Mph1103I ATGCAT 2 cut(s) 1852, 1983
MspA1I CMGCKG 2 cut(s) 1030, 2203
MspCI CTTAAG 2 cut(s) 2554, 2605
MspR9I CCNGG 4 cut(s) 1443, 1752, 2078, 2314
MunI CAATTG 2 cut(s) 16, 2030
Mva1269I GAATGC 1 cut(s) 1321
MvaI CCWGG 4 cut(s) 1443, 1752, 2078, 2314
MvnI CGCG 2 cut(s) 1018, 1290
MwoI GCNNNNNNNGC 3 cut(s) 569, 671, 2206
NlaIV GGNNCC 7 cut(s) 23, 61, 540, 541, 924, 1912, 2385
NmeAIII GCCGAG 1 cut(s) 1674
NmuCI GTSAC 3 cut(s) 97, 774, 1734
NsiI ATGCAT 2 cut(s) 1852, 1983
NspI RCATGY 2 cut(s) 994, 1985
NspV TTCGAA 1 cut(s) 2090
PagI TCATGA 3 cut(s) 1311, 1672, 2592
PciI ACATGT 1 cut(s) 990
PciSI GCTCTTC 1 cut(s) 1510
PcsI WCGNNNNNNNCGW 1 cut(s) 2096
PctI GAATGC 1 cut(s) 1321
PfeI GAWTC 8 cut(s) 107, 133, 476, 1337, 1474, 1967, 2418, 2619
Pfl23II CGTACG 1 cut(s) 450
PflFI GACNNNGTC 1 cut(s) 1577
PflMI CCANNNNNTGG 1 cut(s) 1448
PfoI TCCNGGA 1 cut(s) 2076
PkrI GCNGC 3 cut(s) 871, 1015, 2202
PleI GAGTC 2 cut(s) 1028, 1811
PpsI GAGTC 2 cut(s) 1028, 1811
Ppu21I YACGTR 1 cut(s) 2173
PpuMI RGGWCCY 3 cut(s) 539, 614, 2384
PscI ACATGT 1 cut(s) 990
PshAI GACNNNNGTC 1 cut(s) 537
Psp124BI GAGCTC 1 cut(s) 740
Psp1406I AACGTT 2 cut(s) 730, 1278
Psp5II RGGWCCY 3 cut(s) 539, 614, 2384
Psp6I CCWGG 4 cut(s) 1441, 1750, 2076, 2312
PspEI GGTNACC 1 cut(s) 2316
PspFI CCCAGC 1 cut(s) 274
PspGI CCWGG 4 cut(s) 1441, 1750, 2076, 2312
PspLI CGTACG 1 cut(s) 450
PspN4I GGNNCC 7 cut(s) 23, 61, 540, 541, 924, 1912, 2385
PspPI GGNCC 6 cut(s) 59, 539, 614, 1205, 2080, 2384
PspPPI RGGWCCY 3 cut(s) 539, 614, 2384
PsuI RGATCY 8 cut(s) 35, 46, 424, 1039, 1117, 1219, 1342, 1783
PsyI GACNNNGTC 1 cut(s) 1577
PvuII CAGCTG 2 cut(s) 1030, 2203
RsaI GTAC 4 cut(s) 23, 452, 984, 2132
RsaNI GTAC 4 cut(s) 22, 451, 983, 2131
SacI GAGCTC 1 cut(s) 740
SalI GTCGAC 1 cut(s) 1814
SapI GCTCTTC 1 cut(s) 1510
SatI GCNGC 3 cut(s) 870, 1014, 2201
Sau96I GGNCC 6 cut(s) 59, 539, 614, 1205, 2080, 2384
SchI GAGTC 2 cut(s) 1029, 1811
ScrFI CCNGG 4 cut(s) 1443, 1752, 2078, 2314
SduI GDGCHC 3 cut(s) 740, 747, 1522
SexAI ACCWGGT 1 cut(s) 2312
SfaNI GCATC 5 cut(s) 629, 1209, 1837, 1859, 2559
SfcI CTRYAG 3 cut(s) 567, 1123, 2413
SfuI TTCGAA 1 cut(s) 2090
SinI GGWCC 6 cut(s) 59, 539, 614, 1205, 2080, 2384
SmlI CTYRAG 4 cut(s) 609, 1954, 2554, 2605
SmoI CTYRAG 4 cut(s) 609, 1954, 2554, 2605
SnaBI TACGTA 1 cut(s) 2173
SsiI CCGC 4 cut(s) 39, 173, 335, 342
SspI AATATT 1 cut(s) 2545
SspMI CTAG 5 cut(s) 1608, 2259, 2340, 2448, 2635
SstI GAGCTC 1 cut(s) 740
StyD4I CCNGG 4 cut(s) 1441, 1750, 2076, 2312
StyI CCWWGG 1 cut(s) 770
TaaI ACNGT 5 cut(s) 488, 1424, 2066, 2130, 2188
TaiI ACGT 5 cut(s) 555, 733, 808, 1281, 2175
TaqI TCGA 6 cut(s) 66, 375, 474, 1716, 1815, 2090
TaqII GACCGA 1 cut(s) 2097
TatI WGTACW 1 cut(s) 2130
TfiI GAWTC 8 cut(s) 107, 133, 476, 1337, 1474, 1967, 2418, 2619
TscAI CASTG 8 cut(s) 118, 606, 747, 1427, 1737, 1780, 2069, 2501
TseFI GTSAC 3 cut(s) 97, 774, 1734
TseI GCWGC 3 cut(s) 869, 1013, 2200
Tsp45I GTSAC 3 cut(s) 97, 774, 1734
TspGWI ACGGA 4 cut(s) 116, 343, 1154, 1888
TspRI CASTG 8 cut(s) 118, 606, 747, 1427, 1737, 1780, 2069, 2501
Tth111I GACNNNGTC 1 cut(s) 1577
Van91I CCANNNNNTGG 1 cut(s) 1448
Vha464I CTTAAG 2 cut(s) 2554, 2605
VpaK11BI GGWCC 6 cut(s) 59, 539, 614, 1205, 2080, 2384
XapI RAATTY 8 cut(s) 432, 460, 647, 755, 913, 1631, 2474, 2506
XbaI TCTAGA 2 cut(s) 2339, 2447
XceI RCATGY 2 cut(s) 994, 1985
XcmI CCANNNNNNNNNTGG 1 cut(s) 1264
XmiI GTMKAC 4 cut(s) 224, 490, 1815, 2579
XspI CTAG 5 cut(s) 1608, 2259, 2340, 2448, 2635
Zsp2I ATGCAT 2 cut(s) 1852, 1983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.