Rw2G049890

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000562
Physical Location & Seq
Reverse (-)
77456671 .. 77462349
5679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G049890.1

Sequence Viewer

Length: 3165 bp
ATGGCTGGGAAATCTGGTTCTAAAGAGGATGAACCTCTTGCACATTCTTCCGATGTGGATTGTAATCCAAACCAACGTCTTAGCTCTGTTTTGTTGAATGAATTCAACTATCTCTCTTGGGCTAGAGCTGTTACACTTGCTCTTGGAGGAAGGTCTAAGCTTGGCTATGTCAATGGAGTTATACAGAAGCCTAAAGCCGACTCTCCTACATTTGATTCTTGGCTGTGCAAGGATCAACTCGTCATGTCGTGGCTAATCAACTCCATGGAGAGCAGAATAGCAGAAATTTTCAGTTTTTCTGAGTCTTCTATGCATCTCTGGAATCATGTCAAAGAGATGTATGGAAATCAGAATAATGCAGCTCGAGTTTTCCAACTCAAACGAGATATTGCTGATCTTCAGCAAGAAGGTAAACCCTTTGTTCAGCATCTTGGTAGCCTTACAAGTATGTGGAATGAGCTAGATGTTTATAGACCTCATACTACTGAGGCTAGTGTTTTGTTAAAAAGAGCTGAGGAGGACAAGATTTTTCAGCTCCTTGCAAGCTTAAGTTCAGAATATGAAGATTTAAGAAGTCATATTCTTATGAATACTGAGCTTCCATCTTTCAACATTGTGTGTGCCACCATTCAAAGAGAAGAAGTTCGAAGAAAGGTGATGAATATGGAGACCAAGACAAGTGCATCTGAAACCAGGGCTTATGCTTCTAATCACAGGCAAGCTGAAGCTAAAGTGTACAAAGGCAAGAGGCTTGACTTGAAATGCAGCTACTGTGAGGGGGTTGGACATGTCAGGGAAAGATGTTGGGTTCTCTATCCTGAGCAGAAGCCAAAATTTCTAAAGGAAGGCAAAAGCTCTCAAAAGAGTTGGAATCCACCAAAAGCAAACCTGGCAACAACTTCTTCTTCCTCTTCTTCTGAGGGTAGGCTCAATTTCACCTCCAATCCCACTACTTTAATCAATGAATTTGCTGCTTATCTAAGCAAGAAGCAGATGCATAGTGGAGGTGAAGAAACAGCCATGCCTGGGATTGAAGGTCACACTGCACTTCTTGGAAAGTTTGCTGGCTTCCTTGCAGAGACTGACTATGTTCCACTTGAAGAAGCTTCAGGTACTCTAAAATCCTTTTCTACTGCTCTCAATGTTTGTGCTATGGATGATTATTGGATTATAGACTCAGGGGCTACGGATCACATGACAAATAAAATCACAAATTTGCATGAGTTTGAAAAATTGTCTATCCCTTCTAAAGTGTCTGTAGCAAATGGCAAAGCACATCAACGTGATAAACTTGATCCTAGAGCTGATAAGTGTGTTTTCCTAGGATATTCATCTACTCAAAAAGGTTATAAATGTTATAACTATGTTACCAGAAAACTAATTGTTTCCAGAGATGTGAGATTTGATGAAGCTACTCCCTATTTCACTAGAAAATCATGTGATTCTAGTCAGGGGGAGTTTCTATCTGATTTGTTTCCAAGTCCTAATCTTCCTATTGATGAGGAGTGCAACTTGCCTGTCAATCCTGATGTTCATTCTGATCCTCCTATGGATGAGGATTGTGACTCGTCTATCAATCCTGACAGTCAAGTACAAGAAGTTCCAGCTGTAATTTCTGATGCAGAAGTTGATATGCAATCTATTATTGAGCCTTCCTCTCCACCAGCAGTTATTCTTCAAAGAAACCCTTTGGTATCTCCACCTGCAGTTGTTCTTCGCAGAAATCCTCCACGACAACGAGTTCCTCCGTCAAAGCTCAATGACTATAAAACCTACACTGCAAGGTATCCGATGACACACTTTATATCTTATAAGAATTTTTCTTCTGCTCATTCTGCATTTCTAAGTGCACTTGATTGTACTCATGAACCTCAAAGTTTTGAGGAAGCTAATCAGCTTGATGTATGGAAGCAAGCTATGGCAGATGAACTTCAAGCACTTCATGACAACAATACATGGAGTATTGTTCGAATTCCTAAAGGGAAGAAGGCTGTAGGCAGTCGTTGGGTTTACAAAACGAAGCTTCATTCGGATGGTTCTGTGGAGAGGCATAAGGCTCGCTTGGTAGCTCGTGGTTTTACTCAAACATATGGAGTAGACTACAAGGAGACTTTTGCTCCTGTTGCTAAAATGAGTACAGTGAGAGTTTTGTTATCAGTTGCAGTCAATCATGCCTGGCCTCTATATCAAATGGATGTAAAAAATGCTTTTTTACATGGTGACAATATTGAAGAGATTAATACTCTTAAGCAATCTTTGCGAAATAGGTTTGCTTTGAAAGATTTGGGCATTCTAAAATATTTTCTTGGTATAGAAGTGGCCACTTCTCAGAAAGGTCTTTTTCTAAACCAAAGAAAATATGTTCTTGATCTTTTACAAGATACTGATCTGCAGGACTGCAAGCCAGCTTGCACTCCTCTTGACAGCAAGCTCCAATTTGATGTACAAAGTAAGCCTCTCTTGAATGTGAGCTATTATCAAAAGCTGGTTGGCAAGCTTATTTATCTTACCATTACTCGTCCCGATATAGCTTATGCAGTAAGCATTGTCAGTCAGTTCATGCATTCTCCCACTTTGGCTCATCTTCACATTGTTAAACGAATTCTTCGTTATCTCAAAGGCTCCGCTGGAAGAGGTGTGTTGTTTAAAAACAATGGTAATACTGAAATCATGGGTTACACAGATGCTGATTGGGCAGGGAACTCTCTTGATCGTAAATCAACTACCGGTTTCTGCACTTTTGTTGGTGGTAACTTAGTTACCTGGAAAAGCAAGAAGCAGACAGTTGTTGCTCGCTCTAGTGCAGAGGCTGAATATAGAGCTATGGCTTCCACTGCTTGTGAGCTCATTTGGTTAAAAGGTCTTCTATCAGATTTGGGGTTTCCTAGTAGTCAACCATTGTCCCTTTTCTGTGACAATCAAGCTGCAATGCATATTGCTTCTAATCCGGTGTTCCATGAAAGAACTAAACACATAGAAGTTGACTGTCACTACATTCGAGCTCAAAAGTTTTTTGTTAAATTTTCGGTTATGAAACTTAATATTTCGGAGCATAATAATATGAAATTTTCGAGCTTTAAAGTTAATAACTTTTTTTTTAGCACACCTACTAGTGCCGTTATCGATCTTCATCCATCTGGCATTAGCTTGCATCAAAATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

1054

Amino Acids

118.57

Weight (kDa)

8.29

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 24 - 65 3.9e-14 gag-polypeptide of LTR copia-type
Retrotran_gag_2 PF14223 49 - 220 6.4e-14 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 389 - 426 4e-06 Pol polyprotein, beta-barrel domain
SH3_retrovirus PF25597 425 - 478 4.8e-22 Retroviral polymerase SH3-like domain
RVT_2 PF07727 650 - 746 3.5e-30 Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_2 PF07727 740 - 807 1e-10 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000562)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17331 FvH4_3g36541
malus_domestica MD07G1078600.v1.1 MD10G1237700.v1.1 MD10G1333400.v1.1 MD11G1102200.v1.1 MD13G1138400.v1.1 MD14G1033400.v1.1 MD15G1053300.v1.1
prunus_persica Prupe.1G465400_v2.0.a1 Prupe.3G136000_v2.0.a1 Prupe.7G119900_v2.0.a1
pyrus_communis pycom01g04630 pycom03g13050 pycom04g21190 pycom05g04910 pycom05g05510 pycom08g15850 pycom09g15040 pycom1094g00010 pycom11g02000 pycom12g06150 pycom13g24110 pycom13g24910 pycom14g00050 pycom15g21970 pycom15g35170 pycom15g35180 pycom16g14620 pycom16g14630 pycom808g00110
rosa_chinensis RchiOBHm_Chr1g0322851 RchiOBHm_Chr1g0365941 RchiOBHm_Chr3g0453341 RchiOBHm_Chr4g0388371 RchiOBHm_Chr4g0394021 RchiOBHm_Chr5g0013181 RchiOBHm_Chr6g0261721 RchiOBHm_Chr6g0261731 RchiOBHm_Chr7g0188861 RchiOBHm_Chr7g0192331 RchiOBHm_Chr7g0231721
rosa_multiflora Rmu_sc0002521.1_g000006 Rmu_sc0003255.1_g000026 Rmu_sc0004191.1_g000036 Rmu_sc0004414.1_g000014 Rmu_sc0005670.1_g000006 Rmu_sc0006081.1_g000001 Rmu_sc0007215.1_g000004 Rmu_sc0010088.1_g000010 Rmu_sc0020285.1_g000003 Rmu_sc0026896.1_g000002 Rmu_ssc0000019.1_g000029
rosa_roxburghii Rroxscaffold_3G00224890 Rroxscaffold_6G00400640 Rroxscaffold_7G00176590 Rroxscaffold_7G00189230
rosa_samantha Rh4BG046700
rosa_wichuraiana Rw1G012690 Rw1G031370 Rw2G042180 Rw2G043670 Rw2G046240 Rw2G049890 Rw2G050760 Rw2G051280 Rw2G053590 Rw3G007590 Rw3G026850 Rw4G001540 Rw4G009760 Rw4G019270 Rw5G000720 Rw5G005620 Rw5G041560 Rw6G007370 Rw6G009830 Rw6G015360 Rw6G031160 Rw7G004930 Rw7G011010 Rw7G024230 Rw7G028240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1350, 1359, 1812
AarI CACCTGC 1 cut(s) 1711
Acc36I ACCTGC 1 cut(s) 1711
AccI GTMKAC 1 cut(s) 2097
AciI CCGC 1 cut(s) 2625
AclWI GGATC 4 cut(s) 240, 1197, 1289, 1535
AcoI YGGCCR 1 cut(s) 2319
AcuI CTGAAG 3 cut(s) 383, 744, 1092
AfaI GTAC 6 cut(s) 737, 1114, 1593, 1861, 2137, 2445
AfiI CCNNNNNNNGG 1 cut(s) 1026
AflII CTTAAG 2 cut(s) 547, 2246
AflIII ACRYGT 1 cut(s) 787
AgeI ACCGGT 1 cut(s) 2726
AhlI ACTAGT 1 cut(s) 3110
AjnI CCWGG 5 cut(s) 692, 888, 1024, 2174, 2762
AloI GAACNNNNNNTCC 1 cut(s) 33
Alw21I GWGCWC 3 cut(s) 1852, 2847, 3004
Alw26I GTCTC 3 cut(s) 662, 1073, 2102
Alw44I GTGCAC 1 cut(s) 1848
AlwI GGATC 4 cut(s) 240, 1197, 1289, 1535
AlwNI CAGNNNCTG 4 cut(s) 771, 1082, 2687, 2810
Ama87I CYCGRG 1 cut(s) 363
AoxI GGCC 2 cut(s) 2177, 2319
ApaLI GTGCAC 1 cut(s) 1848
ApeKI GCWGC 4 cut(s) 359, 765, 971, 2924
AseI ATTAAT 1 cut(s) 2238
AsiGI ACCGGT 1 cut(s) 2726
Asp700I GAANNNNTTC 2 cut(s) 101, 642
AspA2I CCTAGG 1 cut(s) 1321
AsuHPI GGTGA 4 cut(s) 667, 928, 1019, 2231
AsuII TTCGAA 2 cut(s) 646, 1969
AvaI CYCGRG 1 cut(s) 363
AvrII CCTAGG 1 cut(s) 1321
BaeGI GKGCMC 1 cut(s) 1852
BalI TGGCCA 1 cut(s) 2321
BanII GRGCYC 2 cut(s) 2847, 3004
BauI CACGAG 1 cut(s) 2070
BbsI GAAGAC 2 cut(s) 297, 2855
Bbv12I GWGCWC 3 cut(s) 1852, 2847, 3004
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 4 cut(s) 371, 777, 958, 2911
BccI CCATC 3 cut(s) 610, 2027, 3142
BceAI ACGGC 1 cut(s) 3101
BcgI CGANNNNNNTGC 2 cut(s) 2039, 2073
BciT130I CCWGG 5 cut(s) 694, 890, 1026, 2176, 2764
BciVI GTATCC 1 cut(s) 1797
BcoDI GTCTC 3 cut(s) 662, 1073, 2102
BcuI ACTAGT 1 cut(s) 3110
BfmI CTRYAG 4 cut(s) 1257, 1704, 1992, 2390
BfrI CTTAAG 2 cut(s) 547, 2246
BfuAI ACCTGC 1 cut(s) 1711
BfuI GTATCC 1 cut(s) 1797
BisI GCNGC 4 cut(s) 360, 766, 972, 2925
BlnI CCTAGG 1 cut(s) 1321
BlsI GCNGC 4 cut(s) 361, 767, 973, 2926
Bme1390I CCNGG 5 cut(s) 694, 890, 1026, 2176, 2764
BmeT110I CYCGRG 1 cut(s) 363
BmiI GGNNCC 1 cut(s) 2623
BmrFI CCNGG 5 cut(s) 694, 890, 1026, 2176, 2764
BmsI GCATC 7 cut(s) 322, 436, 692, 984, 1609, 2674, 3160
BpiI GAAGAC 2 cut(s) 297, 2855
BplI GAGNNNNNCTC 2 cut(s) 1640, 1672
Bpu10I CCTNAGC 2 cut(s) 513, 819
Bpu14I TTCGAA 2 cut(s) 646, 1969
Bsa29I ATCGAT 1 cut(s) 3123
BsaBI GATNNNNATC 4 cut(s) 63, 1330, 2385, 3129
BsaI GGTCTC 1 cut(s) 662
BsaJI CCNNGG 4 cut(s) 264, 693, 1025, 1321
BsaWI WCCGGW 2 cut(s) 2726, 2947
BsaXI ACNNNNNCTCC 2 cut(s) 2101, 2131
Bsc4I CCNNNNNNNGG 1 cut(s) 1026
Bse118I RCCGGY 1 cut(s) 2726
Bse3DI GCAATG 1 cut(s) 2934
Bse8I GATNNNNATC 4 cut(s) 63, 1330, 2385, 3129
BseBI CCWGG 5 cut(s) 694, 890, 1026, 2176, 2764
BseCI ATCGAT 1 cut(s) 3123
BseDI CCNNGG 4 cut(s) 264, 693, 1025, 1321
BseGI GGATG 6 cut(s) 34, 1162, 1558, 2038, 2200, 3130
BseJI GATNNNNATC 4 cut(s) 63, 1330, 2385, 3129
BseLI CCNNNNNNNGG 1 cut(s) 1026
BseMI GCAATG 1 cut(s) 2934
BseMII CTCAG 8 cut(s) 291, 477, 504, 585, 810, 909, 1191, 2342
BseRI GAGGAG 3 cut(s) 530, 1517, 2406
BseSI GKGCMC 1 cut(s) 1852
BseXI GCAGC 4 cut(s) 371, 777, 958, 2911
BseYI CCCAGC 1 cut(s) 5
BsgI GTGCAG 3 cut(s) 1029, 2719, 2823
BshFI GGCC 2 cut(s) 2179, 2321
BshTI ACCGGT 1 cut(s) 2726
BshVI ATCGAT 1 cut(s) 3123
BsiHKAI GWGCWC 3 cut(s) 1852, 2847, 3004
BsiHKCI CYCGRG 1 cut(s) 363
BsiSI CCGG 2 cut(s) 2727, 2948
BslFI GGGAC 2 cut(s) 2505, 2887
BslI CCNNNNNNNGG 1 cut(s) 1026
BsmAI GTCTC 3 cut(s) 662, 1073, 2102
BsmFI GGGAC 2 cut(s) 2505, 2887
BsmI GAATGC 2 cut(s) 2289, 2563
BsnI GGCC 2 cut(s) 2179, 2321
Bso31I GGTCTC 1 cut(s) 662
BsoBI CYCGRG 1 cut(s) 363
Bsp119I TTCGAA 2 cut(s) 646, 1969
Bsp1286I GDGCHC 3 cut(s) 1852, 2847, 3004
Bsp1407I TGTACA 2 cut(s) 735, 2443
Bsp143I GATC 9 cut(s) 232, 394, 1189, 1294, 1540, 2368, 2386, 2710, 3124
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 1 cut(s) 2625
BspANI GGCC 2 cut(s) 2179, 2321
BspCNI CTCAG 8 cut(s) 292, 478, 505, 586, 811, 910, 1190, 2341
BspDI ATCGAT 1 cut(s) 3123
BspHI TCATGA 2 cut(s) 1864, 1942
BspLI GGNNCC 1 cut(s) 2623
BspMAI CTGCAG 2 cut(s) 1708, 2394
BspMI ACCTGC 1 cut(s) 1711
BspPI GGATC 4 cut(s) 240, 1197, 1289, 1535
BspT104I TTCGAA 2 cut(s) 646, 1969
BspTI CTTAAG 2 cut(s) 547, 2246
BspTNI GGTCTC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 2934
BsrFI RCCGGY 1 cut(s) 2726
BsrGI TGTACA 2 cut(s) 735, 2443
BssAI RCCGGY 1 cut(s) 2726
BssECI CCNNGG 4 cut(s) 264, 693, 1025, 1321
BssMI GATC 9 cut(s) 232, 394, 1189, 1294, 1540, 2368, 2386, 2710, 3124
BssSI CACGAG 1 cut(s) 2070
BssT1I CCWWGG 2 cut(s) 264, 1321
Bst2BI CACGAG 1 cut(s) 2070
Bst2UI CCWGG 5 cut(s) 694, 890, 1026, 2176, 2764
Bst4CI ACNGT 5 cut(s) 773, 1586, 2140, 2785, 2987
Bst6I CTCTTC 3 cut(s) 916, 2226, 2626
BstAFI CTTAAG 2 cut(s) 547, 2246
BstAPI GCANNNNNTGC 1 cut(s) 2257
BstAUI TGTACA 2 cut(s) 735, 2443
BstBI TTCGAA 2 cut(s) 646, 1969
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 6 cut(s) 34, 1162, 1558, 2038, 2200, 3130
BstKTI GATC 9 cut(s) 235, 397, 1192, 1297, 1543, 2371, 2389, 2713, 3127
BstMAI GTCTC 3 cut(s) 662, 1073, 2102
BstMBI GATC 9 cut(s) 232, 394, 1189, 1294, 1540, 2368, 2386, 2710, 3124
BstMWI GCNNNNNNNGC 6 cut(s) 890, 1835, 2123, 2257, 2693, 2834
BstNI CCWGG 5 cut(s) 694, 890, 1026, 2176, 2764
BstNSI RCATGY 1 cut(s) 791
BstSCI CCNGG 5 cut(s) 692, 888, 1024, 2174, 2762
BstSFI CTRYAG 4 cut(s) 1257, 1704, 1992, 2390
BstSLI GKGCMC 1 cut(s) 1852
BstV1I GCAGC 4 cut(s) 371, 777, 958, 2911
BstV2I GAAGAC 2 cut(s) 297, 2855
Bsu15I ATCGAT 1 cut(s) 3123
BsuI GTATCC 1 cut(s) 1797
BsuRI GGCC 2 cut(s) 2179, 2321
BsuTUI ATCGAT 1 cut(s) 3123
BtgI CCRYGG 1 cut(s) 264
BtsCI GGATG 6 cut(s) 34, 1162, 1558, 2038, 2200, 3130
BtsI GCAGTG 3 cut(s) 1041, 1776, 2832
BtsIMutI CAGTG 4 cut(s) 1041, 1776, 2145, 2832
BveI ACCTGC 1 cut(s) 1711
CaiI CAGNNNCTG 4 cut(s) 771, 1082, 2687, 2810
CciI TCATGA 2 cut(s) 1864, 1942
Cfr10I RCCGGY 1 cut(s) 2726
ClaI ATCGAT 1 cut(s) 3123
Csp6I GTAC 6 cut(s) 736, 1113, 1592, 1860, 2136, 2444
CspAI ACCGGT 1 cut(s) 2726
CviQI GTAC 6 cut(s) 736, 1113, 1592, 1860, 2136, 2444
DpnI GATC 9 cut(s) 234, 396, 1191, 1296, 1542, 2370, 2388, 2712, 3126
DpnII GATC 9 cut(s) 232, 394, 1189, 1294, 1540, 2368, 2386, 2710, 3124
DraI TTTAAA 2 cut(s) 2647, 3079
EaeI YGGCCR 1 cut(s) 2319
Eam1104I CTCTTC 3 cut(s) 916, 2226, 2626
EarI CTCTTC 3 cut(s) 916, 2226, 2626
Ecl136II GAGCTC 2 cut(s) 2845, 3002
Eco130I CCWWGG 2 cut(s) 264, 1321
Eco24I GRGCYC 2 cut(s) 2847, 3004
Eco31I GGTCTC 1 cut(s) 662
Eco53kI GAGCTC 2 cut(s) 2845, 3002
Eco57I CTGAAG 3 cut(s) 383, 744, 1092
Eco88I CYCGRG 1 cut(s) 363
EcoICRI GAGCTC 2 cut(s) 2845, 3002
EcoRI GAATTC 3 cut(s) 101, 1971, 2601
EcoRII CCWGG 5 cut(s) 692, 888, 1024, 2174, 2762
EcoT14I CCWWGG 2 cut(s) 264, 1321
EcoT22I ATGCAT 4 cut(s) 315, 999, 2565, 2934
EcoT38I GRGCYC 2 cut(s) 2847, 3004
ErhI CCWWGG 2 cut(s) 264, 1321
FalI AAGNNNNNCTT 6 cut(s) 1672, 1704, 2045, 2077, 2444, 2476
FaqI GGGAC 2 cut(s) 2505, 2887
FauNDI CATATG 1 cut(s) 2089
FblI GTMKAC 1 cut(s) 2097
Fnu4HI GCNGC 4 cut(s) 360, 766, 972, 2925
FokI GGATG 6 cut(s) 41, 1169, 1565, 2045, 2207, 3117
FriOI GRGCYC 2 cut(s) 2847, 3004
Fsp4HI GCNGC 4 cut(s) 360, 766, 972, 2925
GluI GCNGC 4 cut(s) 360, 766, 972, 2925
GsaI CCCAGC 1 cut(s) 9
HaeIII GGCC 2 cut(s) 2179, 2321
HapII CCGG 2 cut(s) 2727, 2948
HincII GTYRAC 2 cut(s) 2894, 2983
HindII GTYRAC 2 cut(s) 2894, 2983
HindIII AAGCTT 5 cut(s) 158, 544, 1104, 2021, 2495
HinfI GANTC 8 cut(s) 200, 215, 302, 322, 871, 1175, 1442, 1565
HpaII CCGG 2 cut(s) 2727, 2948
HphI GGTGA 4 cut(s) 667, 928, 1019, 2231
Hpy166II GTNNAC 7 cut(s) 413, 736, 1850, 2011, 2098, 2894, 2983
Hpy8I GTNNAC 7 cut(s) 413, 736, 1850, 2011, 2098, 2894, 2983
HpyAV CCTTC 7 cut(s) 144, 401, 839, 1028, 1254, 1662, 1981
HpyCH4III ACNGT 5 cut(s) 773, 1586, 2140, 2785, 2987
HpyCH4IV ACGT 2 cut(s) 76, 1282
HpyF10VI GCNNNNNNNGC 6 cut(s) 890, 1835, 2123, 2257, 2693, 2834
HpySE526I ACGT 2 cut(s) 76, 1282
Kzo9I GATC 9 cut(s) 232, 394, 1189, 1294, 1540, 2368, 2386, 2710, 3124
LmnI GCTCC 5 cut(s) 540, 2122, 2436, 2627, 3049
Lsp1109I GCAGC 4 cut(s) 371, 777, 958, 2911
LweI GCATC 7 cut(s) 322, 436, 692, 984, 1609, 2674, 3160
MaeII ACGT 2 cut(s) 76, 1282
MalI GATC 9 cut(s) 234, 396, 1191, 1296, 1542, 2370, 2388, 2712, 3126
MboI GATC 9 cut(s) 232, 394, 1189, 1294, 1540, 2368, 2386, 2710, 3124
MhlI GDGCHC 3 cut(s) 1852, 2847, 3004
MlsI TGGCCA 1 cut(s) 2321
MluNI TGGCCA 1 cut(s) 2321
MlyI GAGTC 4 cut(s) 194, 311, 1169, 1559
MmeI TCCRAC 3 cut(s) 397, 763, 848
Mox20I TGGCCA 1 cut(s) 2321
Mph1103I ATGCAT 4 cut(s) 315, 999, 2565, 2934
MroXI GAANNNNTTC 2 cut(s) 101, 642
MscI TGGCCA 1 cut(s) 2321
MslI CAYNNNNRTG 3 cut(s) 1281, 2031, 3156
Msp20I TGGCCA 1 cut(s) 2321
MspA1I CMGCKG 2 cut(s) 1607, 2627
MspCI CTTAAG 2 cut(s) 547, 2246
MspI CCGG 2 cut(s) 2727, 2948
MspR9I CCNGG 5 cut(s) 694, 890, 1026, 2176, 2764
Mva1269I GAATGC 2 cut(s) 2289, 2563
MvaI CCWGG 5 cut(s) 694, 890, 1026, 2176, 2764
MwoI GCNNNNNNNGC 6 cut(s) 890, 1835, 2123, 2257, 2693, 2834
NcoI CCATGG 1 cut(s) 264
NdeI CATATG 1 cut(s) 2089
NdeII GATC 9 cut(s) 232, 394, 1189, 1294, 1540, 2368, 2386, 2710, 3124
NlaIV GGNNCC 1 cut(s) 2623
NmuCI GTSAC 5 cut(s) 1037, 1562, 2219, 2912, 2987
NsiI ATGCAT 4 cut(s) 315, 999, 2565, 2934
NspI RCATGY 1 cut(s) 791
NspV TTCGAA 2 cut(s) 646, 1969
PaeR7I CTCGAG 1 cut(s) 363
PagI TCATGA 2 cut(s) 1864, 1942
PaqCI CACCTGC 1 cut(s) 1711
PciI ACATGT 1 cut(s) 787
PcsI WCGNNNNNNNCGW 1 cut(s) 2605
PctI GAATGC 2 cut(s) 2289, 2563
PdmI GAANNNNTTC 2 cut(s) 101, 642
PfeI GAWTC 4 cut(s) 215, 322, 871, 1442
PinAI ACCGGT 1 cut(s) 2726
PkrI GCNGC 4 cut(s) 361, 767, 973, 2926
PleI GAGTC 4 cut(s) 194, 310, 1169, 1559
PpsI GAGTC 4 cut(s) 194, 310, 1169, 1559
PscI ACATGT 1 cut(s) 787
PshBI ATTAAT 1 cut(s) 2238
PsiI TTATAA 3 cut(s) 1350, 1359, 1812
Psp124BI GAGCTC 2 cut(s) 2847, 3004
Psp6I CCWGG 5 cut(s) 692, 888, 1024, 2174, 2762
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 5 cut(s) 692, 888, 1024, 2174, 2762
PspN4I GGNNCC 1 cut(s) 2623
PspXI VCTCGAGB 1 cut(s) 363
PsrI GAACNNNNNNTAC 2 cut(s) 1584, 1616
PstI CTGCAG 2 cut(s) 1708, 2394
PstNI CAGNNNCTG 4 cut(s) 771, 1082, 2687, 2810
PvuII CAGCTG 1 cut(s) 1607
RsaI GTAC 6 cut(s) 737, 1114, 1593, 1861, 2137, 2445
RsaNI GTAC 6 cut(s) 736, 1113, 1592, 1860, 2136, 2444
RseI CAYNNNNRTG 3 cut(s) 1281, 2031, 3156
SacI GAGCTC 2 cut(s) 2847, 3004
SatI GCNGC 4 cut(s) 360, 766, 972, 2925
Sau3AI GATC 9 cut(s) 232, 394, 1189, 1294, 1540, 2368, 2386, 2710, 3124
SchI GAGTC 4 cut(s) 194, 311, 1169, 1559
ScrFI CCNGG 5 cut(s) 694, 890, 1026, 2176, 2764
SduI GDGCHC 3 cut(s) 1852, 2847, 3004
SfaNI GCATC 7 cut(s) 322, 436, 692, 984, 1609, 2674, 3160
SfcI CTRYAG 4 cut(s) 1257, 1704, 1992, 2390
Sfr274I CTCGAG 1 cut(s) 363
SfuI TTCGAA 2 cut(s) 646, 1969
SlaI CTCGAG 1 cut(s) 363
SmiMI CAYNNNNRTG 3 cut(s) 1281, 2031, 3156
SmlI CTYRAG 3 cut(s) 363, 547, 2246
SmoI CTYRAG 3 cut(s) 363, 547, 2246
SpeI ACTAGT 1 cut(s) 3110
SsiI CCGC 1 cut(s) 2625
SspI AATATT 3 cut(s) 2227, 2300, 3043
SstI GAGCTC 2 cut(s) 2847, 3004
StyD4I CCNGG 5 cut(s) 692, 888, 1024, 2174, 2762
StyI CCWWGG 2 cut(s) 264, 1321
TaaI ACNGT 5 cut(s) 773, 1586, 2140, 2785, 2987
TaiI ACGT 2 cut(s) 79, 1285
TaqI TCGA 6 cut(s) 364, 646, 1969, 2998, 3071, 3123
TatI WGTACW 5 cut(s) 735, 1591, 1859, 2135, 2443
TfiI GAWTC 4 cut(s) 215, 322, 871, 1442
TscAI CASTG 4 cut(s) 1048, 1783, 2145, 2839
TseFI GTSAC 5 cut(s) 1037, 1562, 2219, 2912, 2987
TseI GCWGC 4 cut(s) 359, 765, 971, 2924
Tsp45I GTSAC 5 cut(s) 1037, 1562, 2219, 2912, 2987
TspGWI ACGGA 2 cut(s) 1202, 1737
TspRI CASTG 4 cut(s) 1048, 1783, 2145, 2839
Vha464I CTTAAG 2 cut(s) 547, 2246
VneI GTGCAC 1 cut(s) 1848
VspI ATTAAT 1 cut(s) 2238
XceI RCATGY 1 cut(s) 791
XhoI CTCGAG 1 cut(s) 363
XmaJI CCTAGG 1 cut(s) 1321
XmiI GTMKAC 1 cut(s) 2097
XmnI GAANNNNTTC 2 cut(s) 101, 642
Zsp2I ATGCAT 4 cut(s) 315, 999, 2565, 2934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.