MD15G1053300.v1.1

gag-polypeptide of LTR copia-type

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000562
Physical Location & Seq
Reverse (-)
3651843 .. 3655040
3198 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1053300.v1.1.491

Sequence Viewer

Length: 2838 bp
ATGGAATTTGTCAGTGAATTGTATTTCTCACCAAAGCAAAAACAACTTCAAATATTCCTCCTCCTTCTTCTCTTGTTTCTCTTAAACCCTAGTGCAACTCCGTTAACATTCAATTTTTCGAGTTTTCCACCCCTCACGGCCAATATATCTCTAGAGGGAGATGCTTACACAGACGGGTTCCTCCGGCTCACCAAAAGCGCTGCGGACGACAACAAGACTCGCAGCGCTGGTCGAGCCACCTATAGCCAACCCTTTCTCCTCCGCCAAAACGCCACAGGAAAGCTCGCTGATTTCACCACAAACTTCACATTTGTCATCGACTCACAAAACGAAACCAACTACGGCAACGGTTTCGCATTCTTCATAGCGCCAAACGCCTCCATACTCAACCGCAAACTAGCGAAGCACACTCTCAGTATGGGCCTTCCCGTACATCCACTTGGGGTCGCCGTGAACACGAGTCTGTATTCCTACGTGGCGGTGGAGTTTGATATCCACTATAACAACCAGTCGGACATCCAAGACCCCGCCGGCGATCATGTTGGTATCGACGTCAACTCGGTCATGTCTAGGATTGCAAAGCCTTGGAATGGAAGTATTGTTAATGGAAGGATCAACACTGCTTTCATTAATTACGATTCCAAATCAAAAAATCTTAGCGTTGCCTTCACTACTTATAATGCAAATGGCGTCCAAGTGATGAGCTATCTCAATTACACGATTGATCTGACTCGTTACTTGCCCGATTTGGTCGTTGTAGGGTTCACTGGTGAAACGGGTTTTCTATATGGTATCAGAGCAGTGATCCATCGTGGACCTGCATCTGTGTTCTTCCGAAATCCTACCAAATTCAAACCAGTTAAAGCCATTATGGCAACATCATCAGAGAAAGTTTCAAAAAACGAAACTATGGATACCAACCCAATTCAACGTTTGAGCTCAGTGCTCCTAAACGAATTCAACTATCTGCCTTGGTCACGAGCTATTTCTCTCGCTCTTGGAGGACGTGGAAAGCTCCAGTTCATTCAAAAGGATGATATCATGCCCGAAACAACTTCACAAGATTATGCAGCATGGGTGTCCCAAGATCAACTTATCATGTCTTGGCTGTTGAATTCTATGGAACCAAAGATGGCTGAGATTTTCAGTTATTCAGTATCATCTCATCACCTTTGGATTTCTGTACGAGACATGTATGGAGATCTAAACAATGCTGCGCGAGTCTTCCAGCTGAAGAAAGATCTTACAGAATTACAACAGGGAAATCTCTCTTTTGTTCAACATCTTGGCAACTTGAAGGCCAAATGGAATGAATTGGATCTCTACAGACCTCATACCACAGACACAACCATTCTTTTAAAGCGTGCTGAAGAAGACAAAGTGTTCCAGTTGCTTGCCAGCATCGGACCAGAATATGAGGACCTTAAAAGTCATCTCCTGATGACACCTGAACTTCCCACCTTCCAGATGGTCTGCAATGTTATTCAACGCGAAGAAATAAGGAAGAAGGTCATGAATGCAGTCGTTGTTGTTGGAGAATCAGATCTTAGGCCATCAGAAGCAAGAGCCTTCACCTCTTTCAGACCATACAAGGGAAAACGACCAGACTTAAAGTGTTCTCACTGTGTGAAAATAGGTCGTCCAGGCATTGGACACATAAAAGAAAAGTGCTGGATTCTTCATCCAGAACTTAAACCCAAGTTCAATGATGATGGAAGAGCACCAAGAAACCAAATGAAGCCTGCCTACACTCCTCAAGGAAACCTTGTTTGCGACAATGTCTCCAAAAATGTGATGAACTCTTCTTCTAGTCCCATTACTCTCATAAATGAATTTGCTAACTTCTTGCAACAGAAACAAGGGACAACAAATCATGAAAATCCTGCAGCCATGCTCGGCAAGTTTGCTGGCTTCCTCGAACAATCAAGCTCAGTGTCACAAAATGATGTGCCAGGTATTGTTGCAACTATTTCCACTGCATTGGATCTTAGTAATAGCCGTGGTTTTTGGATAGTCGACTCAGGAGCTTCTGATCACATGACTAACGATGCATCTTTACTAAATGATTTTCAAAACTTCTCCACTCCTTCTTTTGTCTCCGTAGCTAATGGCACCAAAGTTAGTATAGTAGGCATAGGAAAACTCAAAATCTTCTCAAATAAAAAGGAATCCCTTGTCATGCATGTCCCTTCCTTTCCTTTCAAGTTGCTGTCAGTAGCAAGAATAACACAATTGCTAAATTGTCTTGCAATTTTTTCCACTCACTGTGTGATTTTCCAGGACCGAGTTTCGAAGGAAACGATTGGTAAAGGTTTCTTCCTCAATGGACTGTACTATGTCAACTTCTCTTCAAGTTTTCCAAAAGGTTTCTACGTAAACTCAAACACAGTAAACAAGGAGCAGCTGTGGCACATGAGATTAGCTCATCCTTCTTCCCATGTTATGTCTTTTATTTTTCCTAGTTTCTGTAAAGAGATTTCAGATTGTGAAGTTTGTCAAAAATCTAAGTCCACCAGGTTACCCTTTCCCCATTCTCTCTCTAGAGCTACCAAAGTTTTTGAAATTGTCCATTCCGACATTTGGGGACCTGCAACCTTAGAGTCTTTTGATGGCTACAGATTCTTTATATCTTTCATTGATGATTTCTCTAGAACTACCTTTGTGTATTTACTTAAATTCAAGCACGAAGCTTTCCAGTGTTTCAAAAATTTTCATAACTTAGTTCAAAATCATTTTAACTCTAATATTGGCATCTTAAGGTCTGATAATGGCTCTGAGTATACTTCCAAAATCATGACAACTTACTTAAGTGAGCATGGAATCTTTGCAACAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01110 map00940 map01110
Pfam Domains
Protein Families
Retrotran_gag_2

Protein Analysis

946

Amino Acids

106.21

Weight (kDa)

8.84

Isoelectric Point (pI)

33.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 35 - 261 6e-50 Legume lectin domain
Retrotran_gag_3 PF14244 312 - 343 2.8e-07 gag-polypeptide of LTR copia-type
Retrotran_gag_2 PF14223 341 - 504 4.7e-10 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 670 - 743 3.1e-15 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 776 - 837 9.4e-09 GAG-pre-integrase domain
rve PF00665 851 - 942 1.5e-08 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000562)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17331 FvH4_3g36541
malus_domestica MD07G1078600.v1.1 MD10G1237700.v1.1 MD10G1333400.v1.1 MD11G1102200.v1.1 MD13G1138400.v1.1 MD14G1033400.v1.1 MD15G1053300.v1.1
prunus_persica Prupe.1G465400_v2.0.a1 Prupe.3G136000_v2.0.a1 Prupe.7G119900_v2.0.a1
pyrus_communis pycom01g04630 pycom03g13050 pycom04g21190 pycom05g04910 pycom05g05510 pycom08g15850 pycom09g15040 pycom1094g00010 pycom11g02000 pycom12g06150 pycom13g24110 pycom13g24910 pycom14g00050 pycom15g21970 pycom15g35170 pycom15g35180 pycom16g14620 pycom16g14630 pycom808g00110
rosa_chinensis RchiOBHm_Chr1g0322851 RchiOBHm_Chr1g0365941 RchiOBHm_Chr3g0453341 RchiOBHm_Chr4g0388371 RchiOBHm_Chr4g0394021 RchiOBHm_Chr5g0013181 RchiOBHm_Chr6g0261721 RchiOBHm_Chr6g0261731 RchiOBHm_Chr7g0188861 RchiOBHm_Chr7g0192331 RchiOBHm_Chr7g0231721
rosa_multiflora Rmu_sc0002521.1_g000006 Rmu_sc0003255.1_g000026 Rmu_sc0004191.1_g000036 Rmu_sc0004414.1_g000014 Rmu_sc0005670.1_g000006 Rmu_sc0006081.1_g000001 Rmu_sc0007215.1_g000004 Rmu_sc0010088.1_g000010 Rmu_sc0020285.1_g000003 Rmu_sc0026896.1_g000002 Rmu_ssc0000019.1_g000029
rosa_roxburghii Rroxscaffold_3G00224890 Rroxscaffold_6G00400640 Rroxscaffold_7G00176590 Rroxscaffold_7G00189230
rosa_samantha Rh4BG046700
rosa_wichuraiana Rw1G012690 Rw1G031370 Rw2G042180 Rw2G043670 Rw2G046240 Rw2G049890 Rw2G050760 Rw2G051280 Rw2G053590 Rw3G007590 Rw3G026850 Rw4G001540 Rw4G009760 Rw4G019270 Rw5G000720 Rw5G005620 Rw5G041560 Rw6G007370 Rw6G009830 Rw6G015360 Rw6G031160 Rw7G004930 Rw7G011010 Rw7G024230 Rw7G028240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 678
AatII GACGTC 1 cut(s) 555
Acc36I ACCTGC 2 cut(s) 826, 2596
AccB1I GGYRCC 1 cut(s) 2111
AccB7I CCANNNNNTGG 1 cut(s) 1649
AccI GTMKAC 2 cut(s) 2016, 2780
AccII CGCG 2 cut(s) 1219, 1491
AciI CCGC 5 cut(s) 203, 262, 391, 479, 528
AclI AACGTT 1 cut(s) 931
AclWI GGATC 4 cut(s) 620, 799, 1326, 1992
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 7 cut(s) 5, 848, 956, 1114, 1832, 2675, 2707
AcuI CTGAAG 2 cut(s) 1253, 1389
AcyI GRCGYC 2 cut(s) 552, 690
AdeI CACNNNGTG 2 cut(s) 1627, 2269
AfaI GTAC 3 cut(s) 432, 1185, 2333
AfeI AGCGCT 2 cut(s) 199, 226
AfiI CCNNNNNNNGG 4 cut(s) 590, 1592, 1649, 2580
AflII CTTAAG 2 cut(s) 2755, 2806
AflIII ACRYGT 1 cut(s) 1191
AjiI CACGTC 1 cut(s) 1007
AjnI CCWGG 4 cut(s) 1642, 1951, 2277, 2513
Alw21I GWGCWC 3 cut(s) 941, 948, 1723
Alw26I GTCTC 3 cut(s) 1182, 1786, 2101
AlwI GGATC 4 cut(s) 620, 799, 1326, 1992
Aor51HI AGCGCT 2 cut(s) 199, 226
AoxI GGCC 4 cut(s) 138, 421, 1299, 1550
ApeKI GCWGC 6 cut(s) 200, 222, 1070, 1214, 1886, 2401
ApoI RAATTY 7 cut(s) 5, 848, 956, 1114, 1832, 2675, 2707
AseI ATTAAT 1 cut(s) 630
AspLEI GCGC 4 cut(s) 200, 227, 370, 1219
AspS9I GGNCC 6 cut(s) 421, 815, 1406, 1420, 2281, 2585
AsuHPI GGTGA 6 cut(s) 21, 181, 286, 782, 1160, 1564
AsuII TTCGAA 1 cut(s) 2291
AvaII GGWCC 5 cut(s) 815, 1406, 1420, 2281, 2585
BanI GGYRCC 1 cut(s) 2111
BanII GRGCYC 1 cut(s) 941
BarI GAAGNNNNNNTAC 2 cut(s) 1730, 1762
BauI CACGAG 2 cut(s) 457, 978
BbsI GAAGAC 2 cut(s) 1216, 1380
Bbv12I GWGCWC 3 cut(s) 941, 948, 1723
BbvI GCAGC 6 cut(s) 187, 234, 1082, 1201, 1898, 2413
BccI CCATC 6 cut(s) 816, 1126, 1462, 1561, 1706, 2603
BceAI ACGGC 4 cut(s) 153, 358, 434, 1983
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BciT130I CCWGG 4 cut(s) 1644, 1953, 2279, 2515
BciVI GTATCC 1 cut(s) 907
BclI TGATCA 1 cut(s) 2032
BcoDI GTCTC 3 cut(s) 1182, 1786, 2101
BfaI CTAG 9 cut(s) 90, 152, 398, 570, 1809, 2460, 2541, 2649, 2836
BfmI CTRYAG 4 cut(s) 241, 1324, 1884, 2614
BfoI RGCGCY 3 cut(s) 201, 228, 371
BfrI CTTAAG 2 cut(s) 2755, 2806
BfuAI ACCTGC 2 cut(s) 826, 2596
BfuI GTATCC 1 cut(s) 907
BglI GCCNNNNNGGC 1 cut(s) 872
BglII AGATCT 3 cut(s) 1201, 1240, 1543
BisI GCNGC 6 cut(s) 201, 223, 1071, 1215, 1887, 2402
BlsI GCNGC 6 cut(s) 202, 224, 1072, 1216, 1888, 2403
Bme1390I CCNGG 4 cut(s) 1644, 1953, 2279, 2515
Bme18I GGWCC 5 cut(s) 815, 1406, 1420, 2281, 2585
BmgBI CACGTC 1 cut(s) 1007
BmgT120I GGNCC 6 cut(s) 421, 815, 1406, 1420, 2281, 2585
BmiI GGNNCC 4 cut(s) 179, 1125, 2113, 2586
BmrFI CCNGG 4 cut(s) 1644, 1953, 2279, 2515
BmsI GCATC 6 cut(s) 151, 830, 1410, 2038, 2060, 2760
BpiI GAAGAC 2 cut(s) 1216, 1380
BplI GAGNNNNNCTC 2 cut(s) 2407, 2439
BpmI CTGGAG 1 cut(s) 1001
Bpu14I TTCGAA 1 cut(s) 2291
BpuEI CTTGAG 1 cut(s) 1740
BsaAI YACGTR 2 cut(s) 475, 2374
BsaHI GRCGYC 2 cut(s) 552, 690
BsaJI CCNNGG 3 cut(s) 584, 971, 1999
BsaXI ACNNNNNCTCC 6 cut(s) 240, 270, 1766, 1796, 2390, 2420
Bsc4I CCNNNNNNNGG 4 cut(s) 590, 1592, 1649, 2580
Bse118I RCCGGY 1 cut(s) 530
Bse1I ACTGG 6 cut(s) 508, 772, 857, 1018, 1387, 2695
Bse3DI GCAATG 1 cut(s) 1483
BseBI CCWGG 4 cut(s) 1644, 1953, 2279, 2515
BseDI CCNNGG 3 cut(s) 584, 971, 1999
BseGI GGATG 5 cut(s) 433, 516, 1039, 1681, 2425
BseLI CCNNNNNNNGG 4 cut(s) 590, 1592, 1649, 2580
BseMI GCAATG 1 cut(s) 1483
BseMII CTCAG 6 cut(s) 427, 954, 1128, 1944, 2034, 2766
BseNI ACTGG 6 cut(s) 508, 772, 857, 1018, 1387, 2695
BseRI GAGGAG 3 cut(s) 50, 248, 1743
BseXI GCAGC 6 cut(s) 187, 234, 1082, 1201, 1898, 2413
Bsh1236I CGCG 2 cut(s) 1219, 1491
BshFI GGCC 4 cut(s) 140, 423, 1301, 1552
BshNI GGYRCC 1 cut(s) 2111
BsiHKAI GWGCWC 3 cut(s) 941, 948, 1723
BsiSI CCGG 2 cut(s) 184, 531
BslFI GGGAC 5 cut(s) 1066, 1797, 1876, 2171, 2598
BslI CCNNNNNNNGG 4 cut(s) 590, 1592, 1649, 2580
BsmAI GTCTC 3 cut(s) 1182, 1786, 2101
BsmFI GGGAC 5 cut(s) 1066, 1797, 1876, 2171, 2598
BsmI GAATGC 2 cut(s) 356, 1522
BsnI GGCC 4 cut(s) 140, 423, 1301, 1552
Bsp119I TTCGAA 1 cut(s) 2291
Bsp1286I GDGCHC 3 cut(s) 941, 948, 1723
BspACI CCGC 5 cut(s) 203, 262, 391, 479, 528
BspANI GGCC 4 cut(s) 140, 423, 1301, 1552
BspCNI CTCAG 6 cut(s) 426, 953, 1129, 1943, 2033, 2767
BspFNI CGCG 2 cut(s) 1219, 1491
BspHI TCATGA 3 cut(s) 1512, 1873, 2793
BspLI GGNNCC 4 cut(s) 179, 1125, 2113, 2586
BspMAI CTGCAG 1 cut(s) 1888
BspMI ACCTGC 2 cut(s) 826, 2596
BspPI GGATC 4 cut(s) 620, 799, 1326, 1992
BspQI GCTCTTC 1 cut(s) 1711
BspT104I TTCGAA 1 cut(s) 2291
BspT107I GGYRCC 1 cut(s) 2111
BspTI CTTAAG 2 cut(s) 2755, 2806
BsrDI GCAATG 1 cut(s) 1483
BsrFI RCCGGY 1 cut(s) 530
BsrI ACTGG 6 cut(s) 508, 772, 857, 1018, 1387, 2695
BssAI RCCGGY 1 cut(s) 530
BssECI CCNNGG 3 cut(s) 584, 971, 1999
BssNAI GTATAC 1 cut(s) 2781
BssNI GRCGYC 2 cut(s) 552, 690
BssSI CACGAG 2 cut(s) 457, 978
BssT1I CCWWGG 2 cut(s) 584, 971
Bst1107I GTATAC 1 cut(s) 2781
Bst2BI CACGAG 2 cut(s) 457, 978
Bst2UI CCWGG 4 cut(s) 1644, 1953, 2279, 2515
Bst4CI ACNGT 5 cut(s) 350, 1625, 2267, 2331, 2389
Bst6I CTCTTC 3 cut(s) 1711, 1807, 2353
BstACI GRCGYC 2 cut(s) 552, 690
BstAFI CTTAAG 2 cut(s) 2755, 2806
BstBAI YACGTR 2 cut(s) 475, 2374
BstBI TTCGAA 1 cut(s) 2291
BstC8I GCNNGC 7 cut(s) 285, 532, 1365, 1395, 1399, 1743, 1909
BstDSI CCRYGG 1 cut(s) 1999
BstEII GGTNACC 1 cut(s) 2517
BstF5I GGATG 5 cut(s) 433, 516, 1039, 1681, 2425
BstFNI CGCG 2 cut(s) 1219, 1491
BstH2I RGCGCY 3 cut(s) 201, 228, 371
BstHHI GCGC 4 cut(s) 200, 227, 370, 1219
BstMAI GTCTC 3 cut(s) 1182, 1786, 2101
BstMWI GCNNNNNNNGC 4 cut(s) 233, 374, 872, 2407
BstNI CCWGG 4 cut(s) 1644, 1953, 2279, 2515
BstNSI RCATGY 2 cut(s) 1195, 2186
BstPI GGTNACC 1 cut(s) 2517
BstSCI CCNGG 4 cut(s) 1642, 1951, 2277, 2513
BstSFI CTRYAG 4 cut(s) 241, 1324, 1884, 2614
BstSNI TACGTA 1 cut(s) 2374
BstUI CGCG 2 cut(s) 1219, 1491
BstV1I GCAGC 6 cut(s) 187, 234, 1082, 1201, 1898, 2413
BstV2I GAAGAC 2 cut(s) 1216, 1380
BstX2I RGATCY 5 cut(s) 1201, 1240, 1318, 1543, 1984
BstXI CCANNNNNNTGG 1 cut(s) 1981
BstYI RGATCY 5 cut(s) 1201, 1240, 1318, 1543, 1984
BstZ17I GTATAC 1 cut(s) 2781
BsuI GTATCC 1 cut(s) 907
BsuRI GGCC 4 cut(s) 140, 423, 1301, 1552
BtgI CCRYGG 1 cut(s) 1999
BtrI CACGTC 1 cut(s) 1007
BtsCI GGATG 5 cut(s) 433, 516, 1039, 1681, 2425
BtsI GCAGTG 3 cut(s) 618, 807, 1974
BveI ACCTGC 2 cut(s) 826, 2596
Cac8I GCNNGC 7 cut(s) 285, 532, 1365, 1395, 1399, 1743, 1909
CciI TCATGA 3 cut(s) 1512, 1873, 2793
CfoI GCGC 4 cut(s) 200, 227, 370, 1219
Cfr10I RCCGGY 1 cut(s) 530
Cfr13I GGNCC 6 cut(s) 421, 815, 1406, 1420, 2281, 2585
CseI GACGC 1 cut(s) 679
CsiI ACCWGGT 1 cut(s) 2513
Csp6I GTAC 3 cut(s) 431, 1184, 2332
CviQI GTAC 3 cut(s) 431, 1184, 2332
DraI TTTAAA 1 cut(s) 1359
DraIII CACNNNGTG 2 cut(s) 1627, 2269
EaeI YGGCCR 1 cut(s) 138
Eam1104I CTCTTC 3 cut(s) 1711, 1807, 2353
EarI CTCTTC 3 cut(s) 1711, 1807, 2353
EciI GGCGGA 1 cut(s) 251
Ecl136II GAGCTC 1 cut(s) 939
Eco105I TACGTA 1 cut(s) 2374
Eco130I CCWWGG 2 cut(s) 584, 971
Eco24I GRGCYC 1 cut(s) 941
Eco32I GATATC 2 cut(s) 493, 1039
Eco47I GGWCC 5 cut(s) 815, 1406, 1420, 2281, 2585
Eco47III AGCGCT 2 cut(s) 199, 226
Eco53kI GAGCTC 1 cut(s) 939
Eco57I CTGAAG 2 cut(s) 1253, 1389
Eco91I GGTNACC 1 cut(s) 2517
EcoICRI GAGCTC 1 cut(s) 939
EcoO109I RGGNCCY 2 cut(s) 1420, 2585
EcoO65I GGTNACC 1 cut(s) 2517
EcoRI GAATTC 2 cut(s) 956, 1114
EcoRII CCWGG 4 cut(s) 1642, 1951, 2277, 2513
EcoRV GATATC 2 cut(s) 493, 1039
EcoT14I CCWWGG 2 cut(s) 584, 971
EcoT22I ATGCAT 2 cut(s) 2053, 2184
EcoT38I GRGCYC 1 cut(s) 941
ErhI CCWWGG 2 cut(s) 584, 971
FalI AAGNNNNNCTT 4 cut(s) 1077, 1109, 1749, 1781
FaqI GGGAC 5 cut(s) 1066, 1797, 1876, 2171, 2598
FauI CCCGC 1 cut(s) 535
FbaI TGATCA 1 cut(s) 2032
FblI GTMKAC 2 cut(s) 2016, 2780
Fnu4HI GCNGC 6 cut(s) 201, 223, 1071, 1215, 1887, 2402
FokI GGATG 5 cut(s) 420, 503, 1046, 1668, 2412
FriOI GRGCYC 1 cut(s) 941
Fsp4HI GCNGC 6 cut(s) 201, 223, 1071, 1215, 1887, 2402
FspBI CTAG 9 cut(s) 90, 152, 398, 570, 1809, 2460, 2541, 2649, 2836
GlaI GCGC 4 cut(s) 199, 226, 369, 1218
GluI GCNGC 6 cut(s) 201, 223, 1071, 1215, 1887, 2402
GsuI CTGGAG 1 cut(s) 1001
HaeII RGCGCY 3 cut(s) 201, 228, 371
HaeIII GGCC 4 cut(s) 140, 423, 1301, 1552
HapII CCGG 2 cut(s) 184, 531
HgaI GACGC 1 cut(s) 679
HhaI GCGC 4 cut(s) 200, 227, 370, 1219
Hin1I GRCGYC 2 cut(s) 552, 690
Hin6I GCGC 4 cut(s) 198, 225, 368, 1217
HinP1I GCGC 4 cut(s) 198, 225, 368, 1217
HincII GTYRAC 4 cut(s) 105, 556, 2017, 2341
HindII GTYRAC 4 cut(s) 105, 556, 2017, 2341
HindIII AAGCTT 1 cut(s) 2688
HpaI GTTAAC 1 cut(s) 105
HpaII CCGG 2 cut(s) 184, 531
HphI GGTGA 6 cut(s) 21, 181, 286, 782, 1160, 1564
Hpy99I CGWCG 1 cut(s) 554
HpyCH4III ACNGT 5 cut(s) 350, 1625, 2267, 2331, 2389
HpyCH4IV ACGT 5 cut(s) 474, 552, 931, 1006, 2373
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 374, 872, 2407
HpySE526I ACGT 5 cut(s) 474, 552, 931, 1006, 2373
Hsp92I GRCGYC 2 cut(s) 552, 690
HspAI GCGC 4 cut(s) 198, 225, 368, 1217
KroI GCCGGC 1 cut(s) 530
KroNI GCCGGC 1 cut(s) 532
Ksp22I TGATCA 1 cut(s) 2032
KspAI GTTAAC 1 cut(s) 105
LguI GCTCTTC 1 cut(s) 1711
LmnI GCTCC 4 cut(s) 951, 1020, 2024, 2398
Lsp1109I GCAGC 6 cut(s) 187, 234, 1082, 1201, 1898, 2413
LweI GCATC 6 cut(s) 151, 830, 1410, 2038, 2060, 2760
MabI ACCWGGT 1 cut(s) 2513
MaeI CTAG 9 cut(s) 90, 152, 398, 570, 1809, 2460, 2541, 2649, 2836
MaeII ACGT 5 cut(s) 474, 552, 931, 1006, 2373
MaeIII GTNAC 4 cut(s) 734, 975, 1935, 2517
MfeI CAATTG 1 cut(s) 2231
MflI RGATCY 5 cut(s) 1201, 1240, 1318, 1543, 1984
MhlI GDGCHC 3 cut(s) 941, 948, 1723
MlyI GAGTC 7 cut(s) 211, 314, 469, 724, 1230, 2012, 2609
MmeI TCCRAC 3 cut(s) 492, 1513, 2598
Mph1103I ATGCAT 2 cut(s) 2053, 2184
MreI CGCCGGCG 1 cut(s) 530
MroNI GCCGGC 1 cut(s) 530
MspA1I CMGCKG 2 cut(s) 1231, 2404
MspCI CTTAAG 2 cut(s) 2755, 2806
MspI CCGG 2 cut(s) 184, 531
MspR9I CCNGG 4 cut(s) 1644, 1953, 2279, 2515
MunI CAATTG 1 cut(s) 2231
Mva1269I GAATGC 2 cut(s) 356, 1522
MvaI CCWGG 4 cut(s) 1644, 1953, 2279, 2515
MvnI CGCG 2 cut(s) 1219, 1491
MwoI GCNNNNNNNGC 4 cut(s) 233, 374, 872, 2407
NaeI GCCGGC 1 cut(s) 532
NgoMIV GCCGGC 1 cut(s) 530
NlaIV GGNNCC 4 cut(s) 179, 1125, 2113, 2586
NmeAIII GCCGAG 1 cut(s) 1875
NmuCI GTSAC 2 cut(s) 975, 1935
NsiI ATGCAT 2 cut(s) 2053, 2184
NspI RCATGY 2 cut(s) 1195, 2186
NspV TTCGAA 1 cut(s) 2291
PagI TCATGA 3 cut(s) 1512, 1873, 2793
PciI ACATGT 1 cut(s) 1191
PciSI GCTCTTC 1 cut(s) 1711
PcsI WCGNNNNNNNCGW 1 cut(s) 2297
PctI GAATGC 2 cut(s) 356, 1522
PdiI GCCGGC 1 cut(s) 532
PfeI GAWTC 6 cut(s) 638, 1538, 1675, 2168, 2619, 2820
PflFI GACNNNGTC 1 cut(s) 1778
PflMI CCANNNNNTGG 1 cut(s) 1649
PfoI TCCNGGA 1 cut(s) 2277
PkrI GCNGC 6 cut(s) 202, 224, 1072, 1216, 1888, 2403
PleI GAGTC 7 cut(s) 211, 314, 468, 724, 1229, 2012, 2608
PpsI GAGTC 7 cut(s) 211, 314, 468, 724, 1229, 2012, 2608
Ppu21I YACGTR 2 cut(s) 475, 2374
PpuMI RGGWCCY 2 cut(s) 1420, 2585
PscI ACATGT 1 cut(s) 1191
PshBI ATTAAT 1 cut(s) 630
PsiI TTATAA 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 941
Psp1406I AACGTT 1 cut(s) 931
Psp5II RGGWCCY 2 cut(s) 1420, 2585
Psp6I CCWGG 4 cut(s) 1642, 1951, 2277, 2513
PspEI GGTNACC 1 cut(s) 2517
PspGI CCWGG 4 cut(s) 1642, 1951, 2277, 2513
PspN4I GGNNCC 4 cut(s) 179, 1125, 2113, 2586
PspPI GGNCC 6 cut(s) 421, 815, 1406, 1420, 2281, 2585
PspPPI RGGWCCY 2 cut(s) 1420, 2585
PstI CTGCAG 1 cut(s) 1888
PsuI RGATCY 5 cut(s) 1201, 1240, 1318, 1543, 1984
PsyI GACNNNGTC 1 cut(s) 1778
PvuII CAGCTG 2 cut(s) 1231, 2404
RsaI GTAC 3 cut(s) 432, 1185, 2333
RsaNI GTAC 3 cut(s) 431, 1184, 2332
SacI GAGCTC 1 cut(s) 941
SalI GTCGAC 1 cut(s) 2015
SapI GCTCTTC 1 cut(s) 1711
SatI GCNGC 6 cut(s) 201, 223, 1071, 1215, 1887, 2402
Sau96I GGNCC 6 cut(s) 421, 815, 1406, 1420, 2281, 2585
SchI GAGTC 7 cut(s) 211, 314, 469, 724, 1230, 2012, 2609
ScrFI CCNGG 4 cut(s) 1644, 1953, 2279, 2515
SduI GDGCHC 3 cut(s) 941, 948, 1723
SexAI ACCWGGT 1 cut(s) 2513
SfaNI GCATC 6 cut(s) 151, 830, 1410, 2038, 2060, 2760
SfcI CTRYAG 4 cut(s) 241, 1324, 1884, 2614
SfuI TTCGAA 1 cut(s) 2291
SgrAI CRCCGGYG 1 cut(s) 530
SinI GGWCC 5 cut(s) 815, 1406, 1420, 2281, 2585
SmlI CTYRAG 3 cut(s) 1755, 2755, 2806
SmoI CTYRAG 3 cut(s) 1755, 2755, 2806
SnaBI TACGTA 1 cut(s) 2374
SsiI CCGC 5 cut(s) 203, 262, 391, 479, 528
SspI AATATT 2 cut(s) 54, 2746
SspMI CTAG 9 cut(s) 90, 152, 398, 570, 1809, 2460, 2541, 2649, 2836
SstI GAGCTC 1 cut(s) 941
StyD4I CCNGG 4 cut(s) 1642, 1951, 2277, 2513
StyI CCWWGG 2 cut(s) 584, 971
TaaI ACNGT 5 cut(s) 350, 1625, 2267, 2331, 2389
TaiI ACGT 5 cut(s) 477, 555, 934, 1009, 2376
TaqI TCGA 7 cut(s) 119, 232, 318, 549, 1917, 2016, 2291
TaqII GACCGA 2 cut(s) 550, 2298
TatI WGTACW 1 cut(s) 2331
TfiI GAWTC 6 cut(s) 638, 1538, 1675, 2168, 2619, 2820
TseFI GTSAC 2 cut(s) 975, 1935
TseI GCWGC 6 cut(s) 200, 222, 1070, 1214, 1886, 2401
Tsp45I GTSAC 2 cut(s) 975, 1935
TspGWI ACGGA 2 cut(s) 90, 2089
Tth111I GACNNNGTC 1 cut(s) 1778
Van91I CCANNNNNTGG 1 cut(s) 1649
Vha464I CTTAAG 2 cut(s) 2755, 2806
VpaK11BI GGWCC 5 cut(s) 815, 1406, 1420, 2281, 2585
VspI ATTAAT 1 cut(s) 630
XapI RAATTY 7 cut(s) 5, 848, 956, 1114, 1832, 2675, 2707
XbaI TCTAGA 3 cut(s) 151, 2540, 2648
XceI RCATGY 2 cut(s) 1195, 2186
XcmI CCANNNNNNNNNTGG 1 cut(s) 1465
XmiI GTMKAC 2 cut(s) 2016, 2780
XspI CTAG 9 cut(s) 90, 152, 398, 570, 1809, 2460, 2541, 2649, 2836
ZraI GACGTC 1 cut(s) 553
Zsp2I ATGCAT 2 cut(s) 2053, 2184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.