Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000562

Physical Location & Seq

Genomic Coordinates Forward (+)

8235527 .. 8240526

Gene Structure 5000 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ16867

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3051 bp

ATGGCTCAGTTTACTGCTACAGTATGGCTGCAGCATGGTTTTCTGCTGTGCAAGGATCAACTTGCCATGTCTTGGCTAATCAACTCCATGGAGAGTAGAATAGCAGAAATTTTCAGTTTTTCTGAGTCATCTATGCATCTCTGGAATAATGTCAAGGAGATGTATGGAAATCAGAATAATGCTGCTCGAGTTTTTCAACTCAAACGAGATATTGCCAATTTTCAACAAGAAGGTAAGCCCTTTGTTCAGCATCTTGGTAGTCTTACAAGTATGTGGAATGAGCTAGATGTTTATAGACCTCATACTATCGAGGCTAGTGTTTTGTTAAAAAGAGCTGAGGAGGATAAGATCTTTCAGCTTCTTACAAGTTTAAGCTCAGAATATGAAGATTTGAGAAGTCATATTCTTATGAATACTGAGCTACCGTCTTTCAACAATGTGTGTGCCACCATTCAAAGAGAAGAAGTTCAAAAAAAGGTGATGAATATGGAGACCAAGACAAGTGCATCTGAAACCAGAGCTTATGCTTCTAATCACAGGCAAGCTGAAGCTAAAGTGTACAAGGGCAAGAGGCCTGACTTGAAATGCAGCTACTGTGAGGGGGTTGGACATGTCAGGGAAAGATGTGGGGTTCTCTATCCAGAGCAAAAGCCAAAATTTCTAAAGGAAGGCAAAAGCTCTCAAAAGAGTTGGAATCCACCAAAAGCAAACTTGGTAGCAACTTCTTCTTCCTCTTCTTCTGAGGGTAGGCTCAACTTCACCTCCAATCTCACTACTTTAATCAATGAATTTGCTGCTTATCTCAACAAGAAGCAGATGCATAGTGGAGATGAAGAAACAGCCATGCCTGGGATTGAAGGTCACACCACACTCCTTGGAAAGCTTGCTGGCTTCCTTGCAGAGACTGATTATGTTCCACATGAAGAAGCTTCAGATAATTTTACGAGGATTACTTGGTTGTATTTATTGAAATTCAAAAGTGAAGTTGTGGATGTTTTAAAGGATTTTCATAAACTTGTCATCACTCAATTTTCTTCATCCATTCATGTGTTACGATCCGATAATGGCTCTGAGTATATGTCCAATAATATGTCAAATTACTTGAGCACACAAGGCATTATGCACCAAACCAGCTGTGTTGGTACTCCTCAACAGAACGGGATTGCTGAGAGAAAGAATCGAGACCTCCTTGAAAAAGCAAGAGCACTTATGTTTCAAATGAATGTTCCAAAAAAATTTTGGTCTCAAGGTGTTCTCACTGCCACATATCTCATGAACAGACTCCCTAGTAGAGTGCTTGGTTTCAAGTCACCTCTTGAAGTGTTGAAAGGAAGAAAGATTGATTTGTCTCACTTAAAAGTCTTTGGCTGCACATGTTTTTTTCATATTCAAGCTCATCAACGTGATAAACTTGATCCTAGAGCTGATAAGTGTGTTTTCCTGGGATACTCATCTACTCAAAAGGGTTATAAATGTTATAACTATGTAACCAGGAAACTAATTGTTTCCAGGGATGTGAGATTTGATGAAGCTACTCCCTATTTCACTAGAAAATCATATGATTTTGGTCAAGGGGAGTTTCTCTCTGATTTGTTTCCGAGTCCTAACCTTCCTATTAATGAGGATTGCAGTTCGTTTTTAAATCCTGACACTTTAGTTCAAGAAGTTCCAGCAGTAATCACTGATGCAGAAACTGGTATGCAAGAAGATCAGGAAGTTCCAGCGGTATCCTCTGATATAGAGACTGATACTCAACCTGTTGATGAGCCTACTTCTCCACCTGCAGTTGTTCTTCGTAGAAATCCCTTAGTATCACCTCCTACAGTTGCTCTTCGAAGGAATCCTCCACGAGATCGAGGTCCCCCATCCAAGCTAAATGACTACAAAAACTATACTGCAAGGTATCCAATGACACACTTCATATCCTATAAAAAATTCTCTTCGGCTCATTCTGCTTTTCTAAATGTTCTTGATAGTACTCATGAACCTCAAATATTTGAGGAAGCTAATCATTTTGATGTATGGAAGAAAGCTATGGTAGATGAACTTCAAGCTCTCCATGACAATAATACATGGAGTATGGATGTGAAAAATGCCTTCTTACATGGTGATCTTAAAGAGGAGGTCTATATGAAATTACCTCCTGGTCATCCTCAATCTCATGATCCTGAAATAGTATGCAAGTTGCACAAGTCCATTTATGGTTTAAAGCAATCCCCTCGTACCTGGTATGCTAAGCTCAGTTCAGTACTTGAGGAAGTTGGTTTTCACATAAGCAATGCAGATTCTTCCTTATTTGTTCGAATTGGTTCAACCAGTAAACTTGTGGTGCTCATCTATGTTGATGATTTGATAGTGACTGGTGACAATTTTGAGGAGATTCAGTCTCTCAAGCAATCTCTTCGAAACAAGTTTGCTATTAAAGATTTGGGAATCCTTAAGTACTTTCTTGGCATAGAAATGGCTACTTCTCATAAAGGTCTCTTTCTCAATCAAAGAAAGTACATACTTGATTTACTGCAAGATGCAGATATGGAGGATTGTAAACCAGCTCGCACTCCTCTTGACAGCAAGCTGCAACTTAATGTATTAAGTGAGCCTTTCTTGGATTTAACCTCCTATCAACGACTAGTGGGTAAGCTTATCTATCTCACAATCACTCGACCTGATATTTCTTATGCTGTAAGCATTGTCAGTCAATTCATGCATGCTCCAACTTTAGCTCATCTTCACATTGTGAAGAGAATTCTTCGTTATCTCAAAGGTTATGTTGGAAGAGGTATTTTGATGAAAAACAATGGAAACACTCACATAATGGGATACACCGATGCTGATTGGGCAGGCAATTCACTTGATCAAAAATCAACAATTGGCTTATGTACATTTGTTGGTGGAAATCTGGTTACTTGGAAGAGCAAAAAGCAAACTGTTATTGCTCGTTCAAGTGCTGAGGCTGAGTATAGAGCTATAGCTTCCACTGTTTGTGAGCTTATTTGGTTAAAGGGTCTCCTATCTGATTTGGGATTTCCTAGTTGTCAGCCAATACCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002009 GO:0002165 GO:0003002 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0007154 GO:0007165 GO:0007166 GO:0007219 GO:0007275 GO:0007389 GO:0007444 GO:0007447 GO:0007450 GO:0007472 GO:0007476 GO:0007552 GO:0007560 GO:0008150 GO:0008587 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009953 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0035107 GO:0035114 GO:0035120 GO:0035220 GO:0035222 GO:0035239 GO:0035295 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0048190 GO:0048513 GO:0048518 GO:0048522 GO:0048563 GO:0048569 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0060429 GO:0060562 GO:0065007 GO:0080090 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_34 Helicase_C_4 PHD

Protein Analysis

1016

Amino Acids

115.21

Weight (kDa)

8.13

Isoelectric Point (pI)

43.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Retrotran_gag_2	PF14223	18 - 160	4.3e-12	gag-polypeptide of LTR copia-type
rve	PF00665	312 - 383	2e-06	Integrase core domain
SH3_retrovirus	PF25597	457 - 516	5.8e-26	Retroviral polymerase SH3-like domain
RVT_2	PF07727	687 - 855	2.2e-48	Reverse transcriptase (RNA-dependent DNA polymerase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000562)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g17331 FvH4_3g36541
malus_domestica	MD07G1078600.v1.1 MD10G1237700.v1.1 MD10G1333400.v1.1 MD11G1102200.v1.1 MD13G1138400.v1.1 MD14G1033400.v1.1 MD15G1053300.v1.1
prunus_persica	Prupe.1G465400_v2.0.a1 Prupe.3G136000_v2.0.a1 Prupe.7G119900_v2.0.a1
pyrus_communis	pycom01g04630 pycom03g13050 pycom04g21190 pycom05g04910 pycom05g05510 pycom08g15850 pycom09g15040 pycom1094g00010 pycom11g02000 pycom12g06150 pycom13g24110 pycom13g24910 pycom14g00050 pycom15g21970 pycom15g35170 pycom15g35180 pycom16g14620 pycom16g14630 pycom808g00110
rosa_chinensis	RchiOBHm_Chr1g0322851 RchiOBHm_Chr1g0365941 RchiOBHm_Chr3g0453341 RchiOBHm_Chr4g0388371 RchiOBHm_Chr4g0394021 RchiOBHm_Chr5g0013181 RchiOBHm_Chr6g0261721 RchiOBHm_Chr6g0261731 RchiOBHm_Chr7g0188861 RchiOBHm_Chr7g0192331 RchiOBHm_Chr7g0231721
rosa_multiflora	Rmu_sc0002521.1_g000006 Rmu_sc0003255.1_g000026 Rmu_sc0004191.1_g000036 Rmu_sc0004414.1_g000014 Rmu_sc0005670.1_g000006 Rmu_sc0006081.1_g000001 Rmu_sc0007215.1_g000004 Rmu_sc0010088.1_g000010 Rmu_sc0020285.1_g000003 Rmu_sc0026896.1_g000002 Rmu_ssc0000019.1_g000029
rosa_roxburghii	Rroxscaffold_3G00224890 Rroxscaffold_6G00400640 Rroxscaffold_7G00176590 Rroxscaffold_7G00189230
rosa_samantha	Rh4BG046700
rosa_wichuraiana	Rw1G012690 Rw1G031370 Rw2G042180 Rw2G043670 Rw2G046240 Rw2G049890 Rw2G050760 Rw2G051280 Rw2G053590 Rw3G007590 Rw3G026850 Rw4G001540 Rw4G009760 Rw4G019270 Rw5G000720 Rw5G005620 Rw5G041560 Rw6G007370 Rw6G009830 Rw6G015360 Rw6G031160 Rw7G004930 Rw7G011010 Rw7G024230 Rw7G028240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1470, 1479
AarI	CACCTGC	1 cut(s)	1789
Acc36I	ACCTGC	1 cut(s)	1789
AccB7I	CCANNNNNTGG	1 cut(s)	72
AciI	CCGC	1 cut(s)	1724
AclWI	GGATC	4 cut(s)	63, 1050, 1409, 2159
AcsI	RAATTY	7 cut(s)	108, 656, 788, 971, 1235, 1934, 2745
AcuI	CTGAAG	2 cut(s)	567, 915
AdeI	CACNNNGTG	1 cut(s)	2737
AfaI	GTAC	8 cut(s)	560, 1144, 1978, 2224, 2250, 2444, 2504, 2881
AfiI	CCNNNNNNNGG	2 cut(s)	72, 849
AflII	CTTAAG	1 cut(s)	2438
AflIII	ACRYGT	2 cut(s)	610, 1373
AhlI	ACTAGT	1 cut(s)	2629
AjnI	CCWGG	6 cut(s)	847, 1440, 1490, 1508, 2143, 2225
Alw21I	GWGCWC	3 cut(s)	1109, 1207, 2334
Alw26I	GTCTC	9 cut(s)	485, 896, 1176, 1248, 1353, 1736, 2391, 2486, 3011
AlwI	GGATC	4 cut(s)	63, 1050, 1409, 2159
AlwNI	CAGNNNCTG	3 cut(s)	594, 905, 1694
Ama87I	CYCGRG	1 cut(s)	186
AoxI	GGCC	1 cut(s)	572
ApeKI	GCWGC	7 cut(s)	28, 31, 182, 588, 794, 1368, 2575
ApoI	RAATTY	7 cut(s)	108, 656, 788, 971, 1235, 1934, 2745
AseI	ATTAAT	1 cut(s)	1617
Asp700I	GAANNNNTTC	2 cut(s)	465, 2308
AspS9I	GGNCC	1 cut(s)	1859
AsuHPI	GGTGA	6 cut(s)	490, 751, 1302, 1806, 2120, 2375
AsuII	TTCGAA	3 cut(s)	1834, 2302, 2404
AvaI	CYCGRG	1 cut(s)	186
AvaII	GGWCC	1 cut(s)	1859
BarI	GAAGNNNNNNTAC	2 cut(s)	2031, 2063
BauI	CACGAG	1 cut(s)	1848
Bbv12I	GWGCWC	3 cut(s)	1109, 1207, 2334
BbvCI	CCTCAGC	2 cut(s)	336, 2949
BbvI	GCAGC	7 cut(s)	15, 43, 169, 600, 781, 1355, 2562
BccI	CCATC	1 cut(s)	1873
BcgI	CGANNNNNNTGC	4 cut(s)	2201, 2235, 2384, 2418
BciT130I	CCWGG	6 cut(s)	849, 1442, 1492, 1510, 2145, 2227
BciVI	GTATCC	4 cut(s)	1439, 1738, 1914, 2813
BclI	TGATCA	1 cut(s)	2854
BcoDI	GTCTC	9 cut(s)	485, 896, 1176, 1248, 1353, 1736, 2391, 2486, 3011
BcuI	ACTAGT	1 cut(s)	2629
BfaI	CTAG	7 cut(s)	284, 315, 1287, 1419, 1548, 2630, 3030
BfmI	CTRYAG	5 cut(s)	18, 29, 1782, 1821, 2967
BfrI	CTTAAG	1 cut(s)	2438
BfuAI	ACCTGC	1 cut(s)	1789
BfuI	GTATCC	4 cut(s)	1439, 1738, 1914, 2813
BglII	AGATCT	1 cut(s)	348
BisI	GCNGC	7 cut(s)	29, 32, 183, 589, 795, 1369, 2576
BlpI	GCTNAGC	1 cut(s)	2235
BlsI	GCNGC	7 cut(s)	30, 33, 184, 590, 796, 1370, 2577
BmcAI	AGTACT	3 cut(s)	1978, 2250, 2444
Bme1390I	CCNGG	6 cut(s)	849, 1442, 1492, 1510, 2145, 2227
Bme18I	GGWCC	1 cut(s)	1859
BmeT110I	CYCGRG	1 cut(s)	186
BmgT120I	GGNCC	1 cut(s)	1859
BmiI	GGNNCC	1 cut(s)	1861
BmrFI	CCNGG	6 cut(s)	849, 1442, 1492, 1510, 2145, 2227
BmsI	GCATC	7 cut(s)	145, 259, 515, 807, 1675, 2515, 2818
BplI	GAGNNNNNCTC	2 cut(s)	1568, 1600
Bpu10I	CCTNAGC	2 cut(s)	336, 2949
Bpu1102I	GCTNAGC	1 cut(s)	2235
Bpu14I	TTCGAA	3 cut(s)	1834, 2302, 2404
BpuEI	CTTGAG	4 cut(s)	1123, 1230, 2273, 2375
BsaBI	GATNNNNATC	1 cut(s)	1450
BsaI	GGTCTC	5 cut(s)	485, 1176, 1248, 2486, 3011
BsaJI	CCNNGG	5 cut(s)	87, 848, 874, 1441, 1509
BsaXI	ACNNNNNCTCC	4 cut(s)	746, 776, 2369, 2399
Bsc4I	CCNNNNNNNGG	2 cut(s)	72, 849
Bse1I	ACTGG	3 cut(s)	1699, 2316, 2365
Bse3DI	GCAATG	1 cut(s)	2284
Bse8I	GATNNNNATC	1 cut(s)	1450
BseBI	CCWGG	6 cut(s)	849, 1442, 1492, 1510, 2145, 2227
BseDI	CCNNGG	5 cut(s)	87, 848, 874, 1441, 1509
BseGI	GGATG	6 cut(s)	997, 1037, 1519, 1865, 2089, 2149
BseJI	GATNNNNATC	1 cut(s)	1450
BseLI	CCNNNNNNNGG	2 cut(s)	72, 849
BseMI	GCAATG	1 cut(s)	2284
BseNI	ACTGG	3 cut(s)	1699, 2316, 2365
BseRI	GAGGAG	5 cut(s)	353, 1137, 2135, 2390, 2550
BseXI	GCAGC	7 cut(s)	15, 43, 169, 600, 781, 1355, 2562
BsgI	GTGCAG	1 cut(s)	1354
BshFI	GGCC	1 cut(s)	574
BsiHKAI	GWGCWC	3 cut(s)	1109, 1207, 2334
BsiHKCI	CYCGRG	1 cut(s)	186
BslFI	GGGAC	1 cut(s)	1845
BslI	CCNNNNNNNGG	2 cut(s)	72, 849
BsmAI	GTCTC	9 cut(s)	485, 896, 1176, 1248, 1353, 1736, 2391, 2486, 3011
BsmFI	GGGAC	1 cut(s)	1845
BsnI	GGCC	1 cut(s)	574
Bso31I	GGTCTC	5 cut(s)	485, 1176, 1248, 2486, 3011
BsoBI	CYCGRG	1 cut(s)	186
Bsp119I	TTCGAA	3 cut(s)	1834, 2302, 2404
Bsp1286I	GDGCHC	3 cut(s)	1109, 1207, 2334
Bsp1407I	TGTACA	2 cut(s)	558, 2879
Bsp143I	GATC	9 cut(s)	55, 348, 1055, 1414, 1708, 1852, 2110, 2164, 2854
Bsp1720I	GCTNAGC	1 cut(s)	2235
Bsp19I	CCATGG	1 cut(s)	87
BspACI	CCGC	1 cut(s)	1724
BspANI	GGCC	1 cut(s)	574
BspHI	TCATGA	3 cut(s)	1272, 1981, 2161
BspLI	GGNNCC	1 cut(s)	1861
BspMAI	CTGCAG	2 cut(s)	33, 1786
BspMI	ACCTGC	1 cut(s)	1789
BspPI	GGATC	4 cut(s)	63, 1050, 1409, 2159
BspQI	GCTCTTC	2 cut(s)	1836, 2906
BspT104I	TTCGAA	3 cut(s)	1834, 2302, 2404
BspTI	CTTAAG	1 cut(s)	2438
BspTNI	GGTCTC	5 cut(s)	485, 1176, 1248, 2486, 3011
BsrDI	GCAATG	1 cut(s)	2284
BsrGI	TGTACA	2 cut(s)	558, 2879
BsrI	ACTGG	3 cut(s)	1699, 2316, 2365
BssECI	CCNNGG	5 cut(s)	87, 848, 874, 1441, 1509
BssMI	GATC	9 cut(s)	55, 348, 1055, 1414, 1708, 1852, 2110, 2164, 2854
BssSI	CACGAG	1 cut(s)	1848
BssT1I	CCWWGG	2 cut(s)	87, 874
Bst2BI	CACGAG	1 cut(s)	1848
Bst2UI	CCWGG	6 cut(s)	849, 1442, 1492, 1510, 2145, 2227
Bst4CI	ACNGT	6 cut(s)	22, 426, 596, 1825, 2929, 2980
Bst6I	CTCTTC	7 cut(s)	739, 1836, 1945, 2406, 2735, 2770, 2906
BstAFI	CTTAAG	1 cut(s)	2438
BstAUI	TGTACA	2 cut(s)	558, 2879
BstBI	TTCGAA	3 cut(s)	1834, 2302, 2404
BstC8I	GCNNGC	7 cut(s)	543, 885, 889, 2554, 2573, 2709, 2842
BstDSI	CCRYGG	1 cut(s)	87
BstF5I	GGATG	6 cut(s)	997, 1037, 1519, 1865, 2089, 2149
BstKTI	GATC	9 cut(s)	58, 351, 1058, 1417, 1711, 1855, 2113, 2167, 2857
BstMAI	GTCTC	9 cut(s)	485, 896, 1176, 1248, 1353, 1736, 2391, 2486, 3011
BstMBI	GATC	9 cut(s)	55, 348, 1055, 1414, 1708, 1852, 2110, 2164, 2854
BstMWI	GCNNNNNNNGC	3 cut(s)	1113, 1952, 2837
BstNI	CCWGG	6 cut(s)	849, 1442, 1492, 1510, 2145, 2227
BstNSI	RCATGY	3 cut(s)	614, 1377, 2711
BstSCI	CCNGG	6 cut(s)	847, 1440, 1490, 1508, 2143, 2225
BstSFI	CTRYAG	5 cut(s)	18, 29, 1782, 1821, 2967
BstV1I	GCAGC	7 cut(s)	15, 43, 169, 600, 781, 1355, 2562
BstX2I	RGATCY	1 cut(s)	348
BstYI	RGATCY	1 cut(s)	348
BsuI	GTATCC	4 cut(s)	1439, 1738, 1914, 2813
BsuRI	GGCC	1 cut(s)	574
BtgI	CCRYGG	1 cut(s)	87
BtsCI	GGATG	6 cut(s)	997, 1037, 1519, 1865, 2089, 2149
BtsI	GCAGTG	1 cut(s)	1257
BtsIMutI	CAGTG	3 cut(s)	1257, 1680, 2976
BveI	ACCTGC	1 cut(s)	1789
Cac8I	GCNNGC	7 cut(s)	543, 885, 889, 2554, 2573, 2709, 2842
CaiI	CAGNNNCTG	3 cut(s)	594, 905, 1694
CciI	TCATGA	3 cut(s)	1272, 1981, 2161
Cfr13I	GGNCC	1 cut(s)	1859
CsiI	ACCWGGT	1 cut(s)	2225
Csp6I	GTAC	8 cut(s)	559, 1143, 1977, 2223, 2249, 2443, 2503, 2880
CspCI	CAANNNNNGTGG	2 cut(s)	856, 891
CviQI	GTAC	8 cut(s)	559, 1143, 1977, 2223, 2249, 2443, 2503, 2880
DpnI	GATC	9 cut(s)	57, 350, 1057, 1416, 1710, 1854, 2112, 2166, 2856
DpnII	GATC	9 cut(s)	55, 348, 1055, 1414, 1708, 1852, 2110, 2164, 2854
DraI	TTTAAA	3 cut(s)	999, 1641, 2208
DraIII	CACNNNGTG	1 cut(s)	2737
Eam1104I	CTCTTC	7 cut(s)	739, 1836, 1945, 2406, 2735, 2770, 2906
EarI	CTCTTC	7 cut(s)	739, 1836, 1945, 2406, 2735, 2770, 2906
Eco130I	CCWWGG	2 cut(s)	87, 874
Eco147I	AGGCCT	1 cut(s)	574
Eco31I	GGTCTC	5 cut(s)	485, 1176, 1248, 2486, 3011
Eco47I	GGWCC	1 cut(s)	1859
Eco57I	CTGAAG	2 cut(s)	567, 915
Eco88I	CYCGRG	1 cut(s)	186
EcoO109I	RGGNCCY	1 cut(s)	1859
EcoRI	GAATTC	1 cut(s)	2745
EcoRII	CCWGG	6 cut(s)	847, 1440, 1490, 1508, 2143, 2225
EcoT14I	CCWWGG	2 cut(s)	87, 874
EcoT22I	ATGCAT	3 cut(s)	138, 822, 2709
ErhI	CCWWGG	2 cut(s)	87, 874
FalI	AAGNNNNNCTT	2 cut(s)	2584, 2616
FaqI	GGGAC	1 cut(s)	1845
FauNDI	CATATG	1 cut(s)	1558
FbaI	TGATCA	1 cut(s)	2854
Fnu4HI	GCNGC	7 cut(s)	29, 32, 183, 589, 795, 1369, 2576
FokI	GGATG	6 cut(s)	1004, 1024, 1526, 1852, 2096, 2136
Fsp4HI	GCNGC	7 cut(s)	29, 32, 183, 589, 795, 1369, 2576
FspBI	CTAG	7 cut(s)	284, 315, 1287, 1419, 1548, 2630, 3030
GluI	GCNGC	7 cut(s)	29, 32, 183, 589, 795, 1369, 2576
HaeIII	GGCC	1 cut(s)	574
HindIII	AAGCTT	3 cut(s)	881, 927, 2639
HinfI	GANTC	9 cut(s)	125, 694, 1177, 1281, 1600, 1840, 2285, 2380, 2433
HphI	GGTGA	6 cut(s)	490, 751, 1302, 1806, 2120, 2375
Hpy166II	GTNNAC	4 cut(s)	12, 559, 2321, 2546
Hpy8I	GTNNAC	4 cut(s)	12, 559, 2321, 2546
HpyAV	CCTTC	6 cut(s)	224, 662, 851, 1619, 1830, 2107
HpyCH4III	ACNGT	6 cut(s)	22, 426, 596, 1825, 2929, 2980
HpyCH4IV	ACGT	1 cut(s)	1402
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1113, 1952, 2837
HpySE526I	ACGT	1 cut(s)	1402
Ksp22I	TGATCA	1 cut(s)	2854
Kzo9I	GATC	9 cut(s)	55, 348, 1055, 1414, 1708, 1852, 2110, 2164, 2854
LguI	GCTCTTC	2 cut(s)	1836, 2906
LmnI	GCTCC	1 cut(s)	2716
Lsp1109I	GCAGC	7 cut(s)	15, 43, 169, 600, 781, 1355, 2562
LweI	GCATC	7 cut(s)	145, 259, 515, 807, 1675, 2515, 2818
MabI	ACCWGGT	1 cut(s)	2225
MaeI	CTAG	7 cut(s)	284, 315, 1287, 1419, 1548, 2630, 3030
MaeII	ACGT	1 cut(s)	1402
MaeIII	GTNAC	7 cut(s)	860, 1050, 1308, 1486, 2356, 2363, 2902
MalI	GATC	9 cut(s)	57, 350, 1057, 1416, 1710, 1854, 2112, 2166, 2856
MboI	GATC	9 cut(s)	55, 348, 1055, 1414, 1708, 1852, 2110, 2164, 2854
MfeI	CAATTG	1 cut(s)	2868
MflI	RGATCY	1 cut(s)	348
MhlI	GDGCHC	3 cut(s)	1109, 1207, 2334
MlyI	GAGTC	3 cut(s)	134, 1275, 1609
MmeI	TCCRAC	4 cut(s)	586, 671, 2738, 2752
Mph1103I	ATGCAT	3 cut(s)	138, 822, 2709
MroXI	GAANNNNTTC	2 cut(s)	465, 2308
MslI	CAYNNNNRTG	4 cut(s)	1046, 1401, 2016, 2459
MspA1I	CMGCKG	2 cut(s)	1134, 1724
MspCI	CTTAAG	1 cut(s)	2438
MspR9I	CCNGG	6 cut(s)	849, 1442, 1492, 1510, 2145, 2227
MunI	CAATTG	1 cut(s)	2868
MvaI	CCWGG	6 cut(s)	849, 1442, 1492, 1510, 2145, 2227
MwoI	GCNNNNNNNGC	3 cut(s)	1113, 1952, 2837
NcoI	CCATGG	1 cut(s)	87
NdeI	CATATG	1 cut(s)	1558
NdeII	GATC	9 cut(s)	55, 348, 1055, 1414, 1708, 1852, 2110, 2164, 2854
NlaIV	GGNNCC	1 cut(s)	1861
NmuCI	GTSAC	4 cut(s)	860, 1308, 2356, 2363
NsiI	ATGCAT	3 cut(s)	138, 822, 2709
NspI	RCATGY	3 cut(s)	614, 1377, 2711
NspV	TTCGAA	3 cut(s)	1834, 2302, 2404
PaeI	GCATGC	1 cut(s)	2711
PaeR7I	CTCGAG	1 cut(s)	186
PagI	TCATGA	3 cut(s)	1272, 1981, 2161
PaqCI	CACCTGC	1 cut(s)	1789
PceI	AGGCCT	1 cut(s)	574
PciI	ACATGT	2 cut(s)	610, 1373
PciSI	GCTCTTC	2 cut(s)	1836, 2906
PdmI	GAANNNNTTC	2 cut(s)	465, 2308
PfeI	GAWTC	6 cut(s)	694, 1177, 1840, 2285, 2380, 2433
PflMI	CCANNNNNTGG	1 cut(s)	72
PkrI	GCNGC	7 cut(s)	30, 33, 184, 590, 796, 1370, 2577
PleI	GAGTC	3 cut(s)	133, 1275, 1608
PpsI	GAGTC	3 cut(s)	133, 1275, 1608
PpuMI	RGGWCCY	1 cut(s)	1859
PscI	ACATGT	2 cut(s)	610, 1373
PshBI	ATTAAT	1 cut(s)	1617
PsiI	TTATAA	2 cut(s)	1470, 1479
Psp5II	RGGWCCY	1 cut(s)	1859
Psp6I	CCWGG	6 cut(s)	847, 1440, 1490, 1508, 2143, 2225
PspGI	CCWGG	6 cut(s)	847, 1440, 1490, 1508, 2143, 2225
PspN4I	GGNNCC	1 cut(s)	1861
PspPI	GGNCC	1 cut(s)	1859
PspPPI	RGGWCCY	1 cut(s)	1859
PspXI	VCTCGAGB	1 cut(s)	186
PstI	CTGCAG	2 cut(s)	33, 1786
PstNI	CAGNNNCTG	3 cut(s)	594, 905, 1694
PsuI	RGATCY	1 cut(s)	348
PvuII	CAGCTG	1 cut(s)	1134
RsaI	GTAC	8 cut(s)	560, 1144, 1978, 2224, 2250, 2444, 2504, 2881
RsaNI	GTAC	8 cut(s)	559, 1143, 1977, 2223, 2249, 2443, 2503, 2880
RseI	CAYNNNNRTG	4 cut(s)	1046, 1401, 2016, 2459
SapI	GCTCTTC	2 cut(s)	1836, 2906
SatI	GCNGC	7 cut(s)	29, 32, 183, 589, 795, 1369, 2576
Sau3AI	GATC	9 cut(s)	55, 348, 1055, 1414, 1708, 1852, 2110, 2164, 2854
Sau96I	GGNCC	1 cut(s)	1859
ScaI	AGTACT	3 cut(s)	1978, 2250, 2444
SchI	GAGTC	3 cut(s)	134, 1275, 1609
ScrFI	CCNGG	6 cut(s)	849, 1442, 1492, 1510, 2145, 2227
SduI	GDGCHC	3 cut(s)	1109, 1207, 2334
SexAI	ACCWGGT	1 cut(s)	2225
SfaNI	GCATC	7 cut(s)	145, 259, 515, 807, 1675, 2515, 2818
SfcI	CTRYAG	5 cut(s)	18, 29, 1782, 1821, 2967
Sfr274I	CTCGAG	1 cut(s)	186
SfuI	TTCGAA	3 cut(s)	1834, 2302, 2404
SinI	GGWCC	1 cut(s)	1859
SlaI	CTCGAG	1 cut(s)	186
SmiMI	CAYNNNNRTG	4 cut(s)	1046, 1401, 2016, 2459
SmlI	CTYRAG	6 cut(s)	186, 1102, 1245, 2252, 2390, 2438
SmoI	CTYRAG	6 cut(s)	186, 1102, 1245, 2252, 2390, 2438
SpeI	ACTAGT	1 cut(s)	2629
SphI	GCATGC	1 cut(s)	2711
SseBI	AGGCCT	1 cut(s)	574
SsiI	CCGC	1 cut(s)	1724
SspI	AATATT	1 cut(s)	1995
SspMI	CTAG	7 cut(s)	284, 315, 1287, 1419, 1548, 2630, 3030
StuI	AGGCCT	1 cut(s)	574
StyD4I	CCNGG	6 cut(s)	847, 1440, 1490, 1508, 2143, 2225
StyI	CCWWGG	2 cut(s)	87, 874
TaaI	ACNGT	6 cut(s)	22, 426, 596, 1825, 2929, 2980
TaiI	ACGT	1 cut(s)	1405
TaqI	TCGA	8 cut(s)	187, 309, 1180, 1834, 1855, 2302, 2404, 2662
TatI	WGTACW	6 cut(s)	558, 1976, 2248, 2442, 2502, 2879
TfiI	GAWTC	6 cut(s)	694, 1177, 1840, 2285, 2380, 2433
TscAI	CASTG	3 cut(s)	1264, 1687, 2983
TseFI	GTSAC	4 cut(s)	860, 1308, 2356, 2363
TseI	GCWGC	7 cut(s)	28, 31, 182, 588, 794, 1368, 2575
Tsp45I	GTSAC	4 cut(s)	860, 1308, 2356, 2363
TspRI	CASTG	3 cut(s)	1264, 1687, 2983
Van91I	CCANNNNNTGG	1 cut(s)	72
Vha464I	CTTAAG	1 cut(s)	2438
VpaK11BI	GGWCC	1 cut(s)	1859
VspI	ATTAAT	1 cut(s)	1617
XapI	RAATTY	7 cut(s)	108, 656, 788, 971, 1235, 1934, 2745
XceI	RCATGY	3 cut(s)	614, 1377, 2711
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1236, 2323
XhoI	CTCGAG	1 cut(s)	186
XmnI	GAANNNNTTC	2 cut(s)	465, 2308
XspI	CTAG	7 cut(s)	284, 315, 1287, 1419, 1548, 2630, 3030
ZrmI	AGTACT	3 cut(s)	1978, 2250, 2444
Zsp2I	ATGCAT	3 cut(s)	138, 822, 2709

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.