Zinc-dependent metalloprotease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 86 |
| AfaI | GTAC | 1 cut(s) | 153 |
| AgsI | TTSAA | 3 cut(s) | 34, 56, 192 |
| AjnI | CCWGG | 1 cut(s) | 169 |
| AluBI | AGCT | 2 cut(s) | 137, 223 |
| AluI | AGCT | 2 cut(s) | 137, 223 |
| Alw21I | GWGCWC | 1 cut(s) | 187 |
| Alw26I | GTCTC | 1 cut(s) | 156 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 38 |
| AsuHPI | GGTGA | 1 cut(s) | 89 |
| Bbv12I | GWGCWC | 1 cut(s) | 187 |
| BciT130I | CCWGG | 1 cut(s) | 171 |
| BcoDI | GTCTC | 1 cut(s) | 156 |
| BfaI | CTAG | 1 cut(s) | 209 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 20 |
| Bme1390I | CCNGG | 1 cut(s) | 171 |
| BmrFI | CCNGG | 1 cut(s) | 171 |
| BmrI | ACTGGG | 1 cut(s) | 99 |
| BmuI | ACTGGG | 1 cut(s) | 99 |
| BpuEI | CTTGAG | 1 cut(s) | 141 |
| Bse1I | ACTGG | 1 cut(s) | 94 |
| BseBI | CCWGG | 1 cut(s) | 171 |
| BseNI | ACTGG | 1 cut(s) | 94 |
| BsiHKAI | GWGCWC | 1 cut(s) | 187 |
| BsiSI | CCGG | 1 cut(s) | 20 |
| BsmAI | GTCTC | 1 cut(s) | 156 |
| Bsp1286I | GDGCHC | 1 cut(s) | 187 |
| Bsp143I | GATC | 1 cut(s) | 205 |
| BspQI | GCTCTTC | 1 cut(s) | 114 |
| BsrI | ACTGG | 1 cut(s) | 94 |
| BssMI | GATC | 1 cut(s) | 205 |
| Bst2UI | CCWGG | 1 cut(s) | 171 |
| Bst4CI | ACNGT | 1 cut(s) | 120 |
| Bst6I | CTCTTC | 1 cut(s) | 114 |
| BstDEI | CTNAG | 1 cut(s) | 133 |
| BstKTI | GATC | 1 cut(s) | 208 |
| BstMAI | GTCTC | 1 cut(s) | 156 |
| BstMBI | GATC | 1 cut(s) | 205 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 20 |
| BstNI | CCWGG | 1 cut(s) | 171 |
| BstSCI | CCNGG | 1 cut(s) | 169 |
| Csp6I | GTAC | 1 cut(s) | 152 |
| CviAII | CATG | 2 cut(s) | 149, 178 |
| CviJI | RGCY | 5 cut(s) | 14, 23, 137, 212, 223 |
| CviKI_1 | RGCY | 5 cut(s) | 14, 23, 137, 212, 223 |
| CviQI | GTAC | 1 cut(s) | 152 |
| DdeI | CTNAG | 1 cut(s) | 133 |
| DpnI | GATC | 1 cut(s) | 207 |
| DpnII | GATC | 1 cut(s) | 205 |
| Eam1104I | CTCTTC | 1 cut(s) | 114 |
| EarI | CTCTTC | 1 cut(s) | 114 |
| EcoRII | CCWGG | 1 cut(s) | 169 |
| FaeI | CATG | 2 cut(s) | 152, 181 |
| FaiI | YATR | 2 cut(s) | 150, 179 |
| FatI | CATG | 2 cut(s) | 148, 177 |
| FblI | GTMKAC | 1 cut(s) | 86 |
| FspBI | CTAG | 1 cut(s) | 209 |
| HapII | CCGG | 1 cut(s) | 20 |
| Hin1II | CATG | 2 cut(s) | 152, 181 |
| HincII | GTYRAC | 1 cut(s) | 87 |
| HindII | GTYRAC | 1 cut(s) | 87 |
| HinfI | GANTC | 1 cut(s) | 140 |
| HpaII | CCGG | 1 cut(s) | 20 |
| HphI | GGTGA | 1 cut(s) | 89 |
| Hpy166II | GTNNAC | 1 cut(s) | 87 |
| Hpy8I | GTNNAC | 1 cut(s) | 87 |
| Hpy99I | CGWCG | 1 cut(s) | 88 |
| HpyAV | CCTTC | 1 cut(s) | 62 |
| HpyCH4III | ACNGT | 1 cut(s) | 120 |
| HpyCH4IV | ACGT | 1 cut(s) | 83 |
| HpyCH4V | TGCA | 1 cut(s) | 125 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 20 |
| HpyF3I | CTNAG | 1 cut(s) | 133 |
| HpySE526I | ACGT | 1 cut(s) | 83 |
| Hsp92II | CATG | 2 cut(s) | 152, 181 |
| Kzo9I | GATC | 1 cut(s) | 205 |
| LguI | GCTCTTC | 1 cut(s) | 114 |
| LpnPI | CCDG | 8 cut(s) | 33, 60, 73, 75, 111, 129, 156, 183 |
| MaeI | CTAG | 1 cut(s) | 209 |
| MaeII | ACGT | 1 cut(s) | 83 |
| MalI | GATC | 1 cut(s) | 207 |
| MboI | GATC | 1 cut(s) | 205 |
| MboII | GAAGA | 1 cut(s) | 101 |
| MhlI | GDGCHC | 1 cut(s) | 187 |
| MluCI | AATT | 1 cut(s) | 26 |
| MnlI | CCTC | 1 cut(s) | 165 |
| MroXI | GAANNNNTTC | 1 cut(s) | 38 |
| MspI | CCGG | 1 cut(s) | 20 |
| MspR9I | CCNGG | 1 cut(s) | 171 |
| MvaI | CCWGG | 1 cut(s) | 171 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 20 |
| NdeII | GATC | 1 cut(s) | 205 |
| NlaIII | CATG | 2 cut(s) | 152, 181 |
| PciSI | GCTCTTC | 1 cut(s) | 114 |
| PdmI | GAANNNNTTC | 1 cut(s) | 38 |
| PfeI | GAWTC | 1 cut(s) | 140 |
| Psp6I | CCWGG | 1 cut(s) | 169 |
| PspGI | CCWGG | 1 cut(s) | 169 |
| RsaI | GTAC | 1 cut(s) | 153 |
| RsaNI | GTAC | 1 cut(s) | 152 |
| SalI | GTCGAC | 1 cut(s) | 85 |
| SapI | GCTCTTC | 1 cut(s) | 114 |
| Sau3AI | GATC | 1 cut(s) | 205 |
| ScrFI | CCNGG | 1 cut(s) | 171 |
| SduI | GDGCHC | 1 cut(s) | 187 |
| SetI | ASST | 9 cut(s) | 50, 54, 79, 86, 139, 157, 175, 225, 245 |
| SmlI | CTYRAG | 1 cut(s) | 156 |
| SmoI | CTYRAG | 1 cut(s) | 156 |
| Sse9I | AATT | 1 cut(s) | 26 |
| SspMI | CTAG | 1 cut(s) | 209 |
| StyD4I | CCNGG | 1 cut(s) | 169 |
| TaaI | ACNGT | 1 cut(s) | 120 |
| TaiI | ACGT | 1 cut(s) | 86 |
| TaqI | TCGA | 1 cut(s) | 86 |
| TasI | AATT | 1 cut(s) | 26 |
| TfiI | GAWTC | 1 cut(s) | 140 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| XmiI | GTMKAC | 1 cut(s) | 86 |
| XmnI | GAANNNNTTC | 1 cut(s) | 38 |
| XspI | CTAG | 1 cut(s) | 209 |