pycom17g08720

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000768
Physical Location & Seq
Forward (+)
6558582 .. 6562325
3744 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1815 bp
ATGCTAACGAGAAGCATGAGCTTGATCGAAAATAATATGAACCAAGACAGCACGAGAAACACGCATACCATGCATCACTGCCTATATATATGTACATCTTTTGATATCAAATTCACATACATACTCCTAGTAGCTTCAGCTTCTTGCATTCAGCCACATTGCTCCTCGATGCATAATAATACGCCTCTTCGAAGCCATGGAGCTTACAAGCCAAGCATATGGAGTTACGATTTTATTCAGTCCTTGAAGATTGATCATAATACCCTTCAGGATAAAGTATACAAGGATGGGTGGAAGTTCATTGAAGAGAAAGTTAAGCATATGATTGATTACGATGATGATGATACCAACACGCTGACTGCGCTTGAGCTCATTGATGACATCCAACGGTTAGGTTTGGGATATCACTTTGAAGAGGACATAACGAGAGCCCTTGATAAAATATTGAACAGCATTGACGGCCAGGGAACTGACCAGAGTCTCCACCTCACTGCTCTCAGCTTTAGGCTCCTTAGACAACATGGATTTGAGGTCTCCCAAGATGTATTCAGTAAGTTCACGGACGGTAATGGAAGTTTCAAGGCATGGCTTTGCAAGGATGTAAAAGGAATGTTGAGTCTATACGAAGCTTCATATTTTGCTTTCGAAGGAGAGAGCCTGTTGGATGAGGGCCTTGCATTCTCAACCATTTGTCTCAAAAACCTCAGTGGAGCTAATGTTACCAAGGGACTAGCAGAACAAGTGAGTCATGCACTGGAACTTCCACTGCACCATAGAATGCAAAGATTAGAAGCTAGACGGTATATTGAGGCATACAATGAAAGGCCCGATGCAAATCAGGTGCTACTTGAAATTGCCAAGCGAGATTTTAACTGGGTGCAATGCACACTCCAAAGGGATCTTCAAGAAGTATCGAGGTGGTGGGTGGATATAGGCTTGGCAAAAAAGTTGAGCTTCACCAGAGACAGATTGATGGAGTGCTTTTTTTGGTCGGTTGGAATCGTCTTTGAACCTCAACAGAGTCATGCCAGGAAAGGGCTGGCAAAAGTGGCTGCTCTAGTAACCACAATTGATGATGTCTATGATGTTTATGGTACTTTGGATGAACTAGAGCTTTTTACATCTGCAGTTGAAAGATGGGATATCAAAGCTATACAAACTCTCCCAAATTACATGAAGCTTTGTTTTCTAGCTCTTTATAACACAGTGAATGAGATGGTTTATGACACTTTAAAGGAGCAAGGAGAAAACATTCTCCCTTATGTCACAAAAGCTTGGGCTGATTTGTGCAAAGCATTTCTAAAGGAGACAACATGGCGCTATAACAAGAACACACCCACATTTGAAGAATATATTGACAATGCATGGATATCAGTCTCTGGGGTGGTGTTCTTGGTTCATACATATTTTCTCCTCAACCCGAAGGTCACAAAGCAAGCACTTGAATGCTTAGAGAATCATCACAGCCTCTTGCGTTGGCCATCTCTTATTTTCCGGCTTTCCAATGATTTGAGTACTTCAACAGCAGAAGTGGAGAGAGGAGAAACTGCGAACTCAATCTCATGTATGATGCGTGGCAGTCTAAATGTTTCTGAGGAATCTGCTCGTCAATATATAAGTAATTTGGTTGAAAATAGTTGGAAGAAGCTGAACAAAGATGGAGCTTCTGCATCTCCATTTACAAAGCAATTTGTAAAAGCAGCAATAAACCTTGCTCGGATTTCCCAGTGCGTATACCAGTATGGTGATGGGCACGGAGCTCCAGACACAAGAGCAAAGAATCGAATCATATCAGTGATTGTCGATCCCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko01110 map00900 map01110
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

605

Amino Acids

69.2

Weight (kDa)

5.84

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 73 - 252 3e-52 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 310 - 549 5.3e-91 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 349 - 549 4e-57 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1200
AccI GTMKAC 2 cut(s) 279, 1734
AclWI GGATC 2 cut(s) 906, 1799
AcoI YGGCCR 2 cut(s) 460, 1478
AcsI RAATTY 1 cut(s) 110
AcuI CTGAAG 2 cut(s) 120, 251
AfaI GTAC 3 cut(s) 94, 1096, 1516
AfiI CCNNNNNNNGG 1 cut(s) 1035
AjnI CCWGG 2 cut(s) 462, 1028
AjuI GAANNNNNNNTTGG 2 cut(s) 885, 917
Alw21I GWGCWC 2 cut(s) 372, 1762
Alw26I GTCTC 6 cut(s) 485, 538, 698, 957, 1301, 1381
AlwI GGATC 2 cut(s) 906, 1799
AlwNI CAGNNNCTG 1 cut(s) 1379
AoxI GGCC 4 cut(s) 460, 670, 824, 1478
ApeKI GCWGC 2 cut(s) 1052, 1700
ApoI RAATTY 1 cut(s) 110
Asp700I GAANNNNTTC 1 cut(s) 1251
AspLEI GCGC 2 cut(s) 364, 1320
AspS9I GGNCC 2 cut(s) 670, 825
AsuHPI GGTGA 2 cut(s) 949, 1757
AsuII TTCGAA 2 cut(s) 190, 645
BaeGI GKGCMC 1 cut(s) 1755
BalI TGGCCA 1 cut(s) 1480
BanII GRGCYC 3 cut(s) 372, 433, 1762
BauI CACGAG 1 cut(s) 52
Bbv12I GWGCWC 2 cut(s) 372, 1762
BbvI GCAGC 2 cut(s) 1039, 1712
BccI CCATC 7 cut(s) 281, 967, 1131, 1210, 1489, 1652, 1742
BceAI ACGGC 1 cut(s) 475
BciT130I CCWGG 2 cut(s) 464, 1030
BclI TGATCA 1 cut(s) 253
BcoDI GTCTC 6 cut(s) 485, 538, 698, 957, 1301, 1381
BfaI CTAG 6 cut(s) 128, 731, 795, 1058, 1109, 1190
BfmI CTRYAG 1 cut(s) 1125
BfoI RGCGCY 1 cut(s) 1321
BisI GCNGC 2 cut(s) 1053, 1701
BlsI GCNGC 2 cut(s) 1054, 1702
BmcAI AGTACT 1 cut(s) 1516
Bme1390I CCNGG 2 cut(s) 464, 1030
BmgT120I GGNCC 2 cut(s) 670, 825
BmiI GGNNCC 1 cut(s) 509
BmrFI CCNGG 2 cut(s) 464, 1030
BmrI ACTGGG 2 cut(s) 883, 1720
BmsI GCATC 5 cut(s) 82, 159, 820, 1560, 1679
BmuI ACTGGG 2 cut(s) 883, 1720
BoxI GACNNNNGTC 1 cut(s) 477
BpmI CTGGAG 1 cut(s) 1746
Bpu14I TTCGAA 2 cut(s) 190, 645
BpuEI CTTGAG 1 cut(s) 386
BsaBI GATNNNNATC 1 cut(s) 834
BsaI GGTCTC 1 cut(s) 538
BsaJI CCNNGG 3 cut(s) 196, 463, 723
BsaXI ACNNNNNCTCC 4 cut(s) 702, 732, 1146, 1176
Bsc4I CCNNNNNNNGG 1 cut(s) 1035
Bse1I ACTGG 4 cut(s) 759, 878, 1726, 1738
Bse3DI GCAATG 2 cut(s) 157, 887
Bse8I GATNNNNATC 1 cut(s) 834
BseBI CCWGG 2 cut(s) 464, 1030
BseDI CCNNGG 3 cut(s) 196, 463, 723
BseGI GGATG 5 cut(s) 292, 381, 604, 670, 1108
BseJI GATNNNNATC 1 cut(s) 834
BseLI CCNNNNNNNGG 1 cut(s) 1035
BseMI GCAATG 2 cut(s) 157, 887
BseMII CTCAG 3 cut(s) 511, 718, 1584
BseNI ACTGG 4 cut(s) 759, 878, 1726, 1738
BseRI GAGGAG 3 cut(s) 154, 1403, 1554
BseSI GKGCMC 1 cut(s) 1755
BseXI GCAGC 2 cut(s) 1039, 1712
BsgI GTGCAG 1 cut(s) 752
BshFI GGCC 4 cut(s) 462, 672, 826, 1480
BsiHKAI GWGCWC 2 cut(s) 372, 1762
BsiSI CCGG 1 cut(s) 1495
BslFI GGGAC 1 cut(s) 741
BslI CCNNNNNNNGG 1 cut(s) 1035
BsmAI GTCTC 6 cut(s) 485, 538, 698, 957, 1301, 1381
BsmFI GGGAC 1 cut(s) 741
BsmI GAATGC 4 cut(s) 147, 677, 783, 1451
BsnI GGCC 4 cut(s) 462, 672, 826, 1480
Bso31I GGTCTC 1 cut(s) 538
Bsp119I TTCGAA 2 cut(s) 190, 645
Bsp1286I GDGCHC 4 cut(s) 372, 433, 1755, 1762
Bsp1407I TGTACA 1 cut(s) 92
Bsp143I GATC 4 cut(s) 24, 253, 898, 1804
Bsp19I CCATGG 1 cut(s) 196
BspANI GGCC 4 cut(s) 462, 672, 826, 1480
BspCNI CTCAG 3 cut(s) 510, 717, 1585
BspLI GGNNCC 1 cut(s) 509
BspMAI CTGCAG 1 cut(s) 1129
BspPI GGATC 2 cut(s) 906, 1799
BspT104I TTCGAA 2 cut(s) 190, 645
BspTNI GGTCTC 1 cut(s) 538
BsrDI GCAATG 2 cut(s) 157, 887
BsrGI TGTACA 1 cut(s) 92
BsrI ACTGG 4 cut(s) 759, 878, 1726, 1738
BssECI CCNNGG 3 cut(s) 196, 463, 723
BssMI GATC 4 cut(s) 24, 253, 898, 1804
BssNAI GTATAC 2 cut(s) 280, 1735
BssSI CACGAG 1 cut(s) 52
BssT1I CCWWGG 2 cut(s) 196, 723
Bst1107I GTATAC 2 cut(s) 280, 1735
Bst2BI CACGAG 1 cut(s) 52
Bst2UI CCWGG 2 cut(s) 464, 1030
Bst4CI ACNGT 4 cut(s) 390, 566, 801, 1207
Bst6I CTCTTC 3 cut(s) 192, 300, 408
BstAPI GCANNNNNTGC 1 cut(s) 70
BstAUI TGTACA 1 cut(s) 92
BstBI TTCGAA 2 cut(s) 190, 645
BstC8I GCNNGC 2 cut(s) 1041, 1437
BstDEI CTNAG 5 cut(s) 497, 512, 704, 1450, 1593
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 5 cut(s) 292, 381, 604, 670, 1108
BstH2I RGCGCY 1 cut(s) 1321
BstHHI GCGC 2 cut(s) 364, 1320
BstKTI GATC 4 cut(s) 27, 256, 901, 1807
BstMAI GTCTC 6 cut(s) 485, 538, 698, 957, 1301, 1381
BstMBI GATC 4 cut(s) 24, 253, 898, 1804
BstMWI GCNNNNNNNGC 4 cut(s) 70, 361, 459, 1049
BstNI CCWGG 2 cut(s) 464, 1030
BstPAI GACNNNNGTC 1 cut(s) 477
BstSCI CCNGG 2 cut(s) 462, 1028
BstSFI CTRYAG 1 cut(s) 1125
BstSLI GKGCMC 1 cut(s) 1755
BstV1I GCAGC 2 cut(s) 1039, 1712
BstX2I RGATCY 1 cut(s) 898
BstXI CCANNNNNNTGG 1 cut(s) 219
BstYI RGATCY 1 cut(s) 898
BstZ17I GTATAC 2 cut(s) 280, 1735
BsuRI GGCC 4 cut(s) 462, 672, 826, 1480
BtgI CCRYGG 1 cut(s) 196
BtsCI GGATG 5 cut(s) 292, 381, 604, 670, 1108
BtsI GCAGTG 3 cut(s) 76, 489, 764
BtsIMutI CAGTG 8 cut(s) 76, 489, 712, 752, 764, 1212, 1733, 1800
Cac8I GCNNGC 2 cut(s) 1041, 1437
CaiI CAGNNNCTG 1 cut(s) 1379
CfoI GCGC 2 cut(s) 364, 1320
Cfr13I GGNCC 2 cut(s) 670, 825
Csp6I GTAC 3 cut(s) 93, 1095, 1515
CviQI GTAC 3 cut(s) 93, 1095, 1515
DdeI CTNAG 5 cut(s) 497, 512, 704, 1450, 1593
DpnI GATC 4 cut(s) 26, 255, 900, 1806
DpnII GATC 4 cut(s) 24, 253, 898, 1804
DraI TTTAAA 1 cut(s) 1233
EaeI YGGCCR 2 cut(s) 460, 1478
Eam1104I CTCTTC 3 cut(s) 192, 300, 408
EarI CTCTTC 3 cut(s) 192, 300, 408
Ecl136II GAGCTC 2 cut(s) 370, 1760
Eco130I CCWWGG 2 cut(s) 196, 723
Eco24I GRGCYC 3 cut(s) 372, 433, 1762
Eco31I GGTCTC 1 cut(s) 538
Eco32I GATATC 4 cut(s) 106, 404, 1144, 1371
Eco53kI GAGCTC 2 cut(s) 370, 1760
Eco57I CTGAAG 2 cut(s) 120, 251
EcoICRI GAGCTC 2 cut(s) 370, 1760
EcoO109I RGGNCCY 1 cut(s) 670
EcoRII CCWGG 2 cut(s) 462, 1028
EcoRV GATATC 4 cut(s) 106, 404, 1144, 1371
EcoT14I CCWWGG 2 cut(s) 196, 723
EcoT22I ATGCAT 3 cut(s) 75, 174, 1366
EcoT38I GRGCYC 3 cut(s) 372, 433, 1762
ErhI CCWWGG 2 cut(s) 196, 723
FalI AAGNNNNNCTT 2 cut(s) 938, 970
FaqI GGGAC 1 cut(s) 741
FauNDI CATATG 2 cut(s) 218, 321
FbaI TGATCA 1 cut(s) 253
FblI GTMKAC 2 cut(s) 279, 1734
Fnu4HI GCNGC 2 cut(s) 1053, 1701
FokI GGATG 5 cut(s) 299, 368, 611, 677, 1115
FriOI GRGCYC 3 cut(s) 372, 433, 1762
Fsp4HI GCNGC 2 cut(s) 1053, 1701
FspBI CTAG 6 cut(s) 128, 731, 795, 1058, 1109, 1190
GlaI GCGC 2 cut(s) 363, 1319
GluI GCNGC 2 cut(s) 1053, 1701
GsuI CTGGAG 1 cut(s) 1746
HaeII RGCGCY 1 cut(s) 1321
HaeIII GGCC 4 cut(s) 462, 672, 826, 1480
HapII CCGG 1 cut(s) 1495
HhaI GCGC 2 cut(s) 364, 1320
Hin6I GCGC 2 cut(s) 362, 1318
HinP1I GCGC 2 cut(s) 362, 1318
HindIII AAGCTT 3 cut(s) 627, 1178, 1272
HinfI GANTC 9 cut(s) 478, 616, 745, 999, 1021, 1456, 1598, 1780, 1785
HpaII CCGG 1 cut(s) 1495
HphI GGTGA 2 cut(s) 949, 1757
Hpy166II GTNNAC 3 cut(s) 280, 558, 1735
Hpy188I TCNGA 2 cut(s) 1594, 1719
Hpy188III TCNNGA 3 cut(s) 269, 905, 1763
Hpy8I GTNNAC 3 cut(s) 280, 558, 1735
HpyAV CCTTC 3 cut(s) 275, 641, 1417
HpyCH4III ACNGT 4 cut(s) 390, 566, 801, 1207
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 361, 459, 1049
HpyF3I CTNAG 5 cut(s) 497, 512, 704, 1450, 1593
HspAI GCGC 2 cut(s) 362, 1318
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 4 cut(s) 24, 253, 898, 1804
LmnI GCTCC 8 cut(s) 167, 200, 513, 710, 1237, 1661, 1757, 1765
Lsp1109I GCAGC 2 cut(s) 1039, 1712
LweI GCATC 5 cut(s) 82, 159, 820, 1560, 1679
MaeI CTAG 6 cut(s) 128, 731, 795, 1058, 1109, 1190
MaeIII GTNAC 5 cut(s) 224, 718, 1060, 1264, 1426
MalI GATC 4 cut(s) 26, 255, 900, 1806
MboI GATC 4 cut(s) 24, 253, 898, 1804
MboII GAAGA 7 cut(s) 179, 259, 317, 425, 893, 1358, 1654
MfeI CAATTG 1 cut(s) 1068
MflI RGATCY 1 cut(s) 898
MhlI GDGCHC 4 cut(s) 372, 433, 1755, 1762
MlsI TGGCCA 1 cut(s) 1480
MluCI AATT 6 cut(s) 110, 852, 1068, 1168, 1621, 1688
MluNI TGGCCA 1 cut(s) 1480
MlyI GAGTC 4 cut(s) 487, 625, 754, 1030
MmeI TCCRAC 4 cut(s) 409, 642, 976, 1619
Mox20I TGGCCA 1 cut(s) 1480
Mph1103I ATGCAT 3 cut(s) 75, 174, 1366
MroXI GAANNNNTTC 1 cut(s) 1251
MscI TGGCCA 1 cut(s) 1480
MseI TTAA 4 cut(s) 315, 870, 1232, 1813
MslI CAYNNNNRTG 2 cut(s) 1444, 1793
Msp20I TGGCCA 1 cut(s) 1480
MspI CCGG 1 cut(s) 1495
MspR9I CCNGG 2 cut(s) 464, 1030
MunI CAATTG 1 cut(s) 1068
Mva1269I GAATGC 4 cut(s) 147, 677, 783, 1451
MvaI CCWGG 2 cut(s) 464, 1030
MwoI GCNNNNNNNGC 4 cut(s) 70, 361, 459, 1049
NcoI CCATGG 1 cut(s) 196
NdeI CATATG 2 cut(s) 218, 321
NdeII GATC 4 cut(s) 24, 253, 898, 1804
NlaIV GGNNCC 1 cut(s) 509
NmuCI GTSAC 2 cut(s) 1264, 1426
NsiI ATGCAT 3 cut(s) 75, 174, 1366
NspV TTCGAA 2 cut(s) 190, 645
PctI GAATGC 4 cut(s) 147, 677, 783, 1451
PdmI GAANNNNTTC 1 cut(s) 1251
PfeI GAWTC 5 cut(s) 999, 1456, 1598, 1780, 1785
PkrI GCNGC 2 cut(s) 1054, 1702
PleI GAGTC 4 cut(s) 486, 624, 753, 1029
PpsI GAGTC 4 cut(s) 486, 624, 753, 1029
PshAI GACNNNNGTC 1 cut(s) 477
PsiI TTATAA 1 cut(s) 1200
Psp124BI GAGCTC 2 cut(s) 372, 1762
Psp6I CCWGG 2 cut(s) 462, 1028
PspGI CCWGG 2 cut(s) 462, 1028
PspN4I GGNNCC 1 cut(s) 509
PspPI GGNCC 2 cut(s) 670, 825
PstI CTGCAG 1 cut(s) 1129
PstNI CAGNNNCTG 1 cut(s) 1379
PsuI RGATCY 1 cut(s) 898
RsaI GTAC 3 cut(s) 94, 1096, 1516
RsaNI GTAC 3 cut(s) 93, 1095, 1515
RseI CAYNNNNRTG 2 cut(s) 1444, 1793
SacI GAGCTC 2 cut(s) 372, 1762
SaqAI TTAA 4 cut(s) 315, 870, 1232, 1813
SatI GCNGC 2 cut(s) 1053, 1701
Sau3AI GATC 4 cut(s) 24, 253, 898, 1804
Sau96I GGNCC 2 cut(s) 670, 825
ScaI AGTACT 1 cut(s) 1516
SchI GAGTC 4 cut(s) 487, 625, 754, 1030
ScrFI CCNGG 2 cut(s) 464, 1030
SduI GDGCHC 4 cut(s) 372, 433, 1755, 1762
SfaNI GCATC 5 cut(s) 82, 159, 820, 1560, 1679
SfcI CTRYAG 1 cut(s) 1125
SfuI TTCGAA 2 cut(s) 190, 645
SmiMI CAYNNNNRTG 2 cut(s) 1444, 1793
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 6 cut(s) 110, 852, 1068, 1168, 1621, 1688
SspI AATATT 1 cut(s) 444
SspMI CTAG 6 cut(s) 128, 731, 795, 1058, 1109, 1190
SstI GAGCTC 2 cut(s) 372, 1762
StyD4I CCNGG 2 cut(s) 462, 1028
StyI CCWWGG 2 cut(s) 196, 723
TaaI ACNGT 4 cut(s) 390, 566, 801, 1207
TaqI TCGA 7 cut(s) 27, 167, 190, 645, 914, 1783, 1803
TasI AATT 6 cut(s) 110, 852, 1068, 1168, 1621, 1688
TatI WGTACW 2 cut(s) 92, 1514
TfiI GAWTC 5 cut(s) 999, 1456, 1598, 1780, 1785
Tru1I TTAA 4 cut(s) 315, 870, 1232, 1813
Tru9I TTAA 4 cut(s) 315, 870, 1232, 1813
TscAI CASTG 8 cut(s) 83, 496, 712, 759, 771, 1212, 1733, 1800
TseFI GTSAC 2 cut(s) 1264, 1426
TseI GCWGC 2 cut(s) 1052, 1700
Tsp45I GTSAC 2 cut(s) 1264, 1426
TspDTI ATGAA 7 cut(s) 53, 289, 621, 834, 1119, 1190, 1388
TspGWI ACGGA 2 cut(s) 575, 1770
TspRI CASTG 8 cut(s) 83, 496, 712, 759, 771, 1212, 1733, 1800
XapI RAATTY 1 cut(s) 110
XcmI CCANNNNNNNNNTGG 2 cut(s) 1036, 1745
XmiI GTMKAC 2 cut(s) 279, 1734
XmnI GAANNNNTTC 1 cut(s) 1251
XspI CTAG 6 cut(s) 128, 731, 795, 1058, 1109, 1190
ZrmI AGTACT 1 cut(s) 1516
Zsp2I ATGCAT 3 cut(s) 75, 174, 1366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.