RchiOBHm_Chr2g0164531

spliceosomal complex assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000703
Physical Location & Seq
Reverse (-)
79594543 .. 79596023
1481 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1416 bp
ATGTGTGAGGGTTGCAAGCTTGGTAAGCAAACTCATTCTTCACATAGGGTCACTAATTATGTGTCTACTTCACAACCCTTAGATTTAATGCATATGGATCTGGTTGGACCTGTTCAAACTAAAAGCTTAGGTGGTAAGAAGTACATGCTTGTTCTAGTCGATGATTTTTCTAGGTTCACTTGGGTGAAATTCTTGTGTGAAAAATCTGATGCTTTTGACAACTTTCAAAACTTGTGCAAAATGATTTCAAATGAGCAATACTCTTCAAATAAATGCATTGTAAGACTTAGAACTGATCATGGAACTGAATTTGAAAATGCTTCATTTGATGAGTTTTGTAATGATATGGGAATTAAGCATGAGTTCTCGGCTCCCATAACTCCGCAACAAAATGGTGTTGTGGAAAGGAAAAACAGGGTTTTAATTGAAATGGGAAGAGTTATGTTAAACTCTGCTGGTCTTGCTCATACTTTTTGGGCAGAAGCGATTAGCAATGCTTGCTATACCATCAATAGAGTCATCTTTAGACCCGGAACTGAAAAAACTCCTTATGAGGTTTTGAAAGGAAAGAAACCAAATGTGAGCCATTTAAGAGTGTTTGGTAGCCCTTGTTACATCTATAGGGATAGAGAATATCTTGCCAAATTTGATGCTAAAAGCGACAAAGGAGTCTTTCTTGGTTATTCATTGAATAGTAGAGCTTATAGGGTTTATAACAAGAGAACTTGTTCTGTGATGGAAACCATTAATGTCTCTATTGATGACTCAATTGTGCTATCACATACTCCTTGTATAAGTTTTGATCAGGTACTTTTCGAGAGAGAAAAGGATGATGATGTTGCTGCTGAAGGCCAAGAAGATGAGGAAGCTTCAGAGGGGATTGATGATACCTCTGCAATCCAGCCAGTTTACAGAACTGGACAGCAGCAAGTACATAAGGATCATTCCTCTTCTGACATCATTGGTGATGTGAATGATGGTTTGAAAACAAGGAGACAAGCAAAAAGGGTTGTAAGCAATCTCTCTATTCTTTCATGTTTCATTGCAAAGCATCAAGAAGATATTAGCATCATTACATTTTATGGTTTTGTTTCTGTTATAGAACCTAAAAATGCTAAAGAGGCTTTGTGTGATGTTAATTGGATAAATGCCATGCAAGATGAATTGAGTCAATTTGCTAGAAATGATGTGTGGTTTTTGGTTCCTAGACCGGACTCTTCAAATGTCATTGGAACAAAATGGATTTTCAAAAACAAATCTGATGAAAAGGGTCAGATTACCCGCAACAAAGCAAGGCTTGTTGCTCAAGGGTATTCTCAAGTTGAAGGTCTTGATTTTGATGAAACCTTTGCCCCCGTTGCAAGGCTTGAATCTTGCGTTTTTAAATGGTGTTCTTCAAGAAGAAGTGTATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve zf-CCHC

Protein Analysis

471

Amino Acids

53.48

Weight (kDa)

6.44

Isoelectric Point (pI)

35.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
rve PF00665 24 - 128 1.1e-15 Integrase core domain
SH3_retrovirus PF25597 202 - 258 1.6e-14 Retroviral polymerase SH3-like domain
RVT_2 PF07727 395 - 458 6.8e-17 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
AasI GACNNNNNNGTC 1 cut(s) 668
AccI GTMKAC 1 cut(s) 65
AciI CCGC 2 cut(s) 383, 1282
AclWI GGATC 2 cut(s) 105, 948
AcsI RAATTY 3 cut(s) 188, 308, 644
AcuI CTGAAG 2 cut(s) 855, 867
AfaI GTAC 3 cut(s) 143, 810, 933
AfiI CCNNNNNNNGG 1 cut(s) 1362
AleI CACNNNNGTG 1 cut(s) 182
AluBI AGCT 4 cut(s) 19, 126, 701, 869
AluI AGCT 4 cut(s) 19, 126, 701, 869
Alw26I GTCTC 2 cut(s) 757, 988
AlwI GGATC 2 cut(s) 105, 948
AoxI GGCC 1 cut(s) 850
ApeKI GCWGC 2 cut(s) 842, 925
ApoI RAATTY 3 cut(s) 188, 308, 644
AseI ATTAAT 1 cut(s) 747
Asp700I GAANNNNTTC 1 cut(s) 727
AspS9I GGNCC 1 cut(s) 107
AsuC2I CCSGG 1 cut(s) 531
AsuHPI GGTGA 2 cut(s) 196, 977
AvaII GGWCC 1 cut(s) 107
BbvI GCAGC 2 cut(s) 829, 937
BccI CCATC 3 cut(s) 515, 730, 971
BclI TGATCA 2 cut(s) 295, 802
BcnI CCSGG 1 cut(s) 531
BcoDI GTCTC 2 cut(s) 757, 988
BfaI CTAG 4 cut(s) 155, 171, 1179, 1206
BfmI CTRYAG 1 cut(s) 619
BisI GCNGC 2 cut(s) 843, 926
BlsI GCNGC 2 cut(s) 844, 927
Bme1390I CCNGG 1 cut(s) 531
Bme18I GGWCC 1 cut(s) 107
BmgT120I GGNCC 1 cut(s) 107
BmiI GGNNCC 2 cut(s) 372, 1203
BmrFI CCNGG 1 cut(s) 531
BmsI GCATC 4 cut(s) 199, 640, 1060, 1077
BplI GAGNNNNNCTC 2 cut(s) 245, 277
Bpu10I CCTNAGC 1 cut(s) 127
BpuEI CTTGAG 2 cut(s) 1290, 1302
BpuMI CCSGG 1 cut(s) 531
BsaWI WCCGGW 1 cut(s) 1210
Bsc4I CCNNNNNNNGG 1 cut(s) 1362
Bse1I ACTGG 2 cut(s) 905, 922
Bse3DI GCAATG 2 cut(s) 499, 1041
BseGI GGATG 1 cut(s) 835
BseLI CCNNNNNNNGG 1 cut(s) 1362
BseMI GCAATG 2 cut(s) 499, 1041
BseNI ACTGG 2 cut(s) 905, 922
BseXI GCAGC 2 cut(s) 829, 937
BshFI GGCC 1 cut(s) 852
BsiSI CCGG 2 cut(s) 531, 1211
BslI CCNNNNNNNGG 1 cut(s) 1362
BsmAI GTCTC 2 cut(s) 757, 988
BsnI GGCC 1 cut(s) 852
Bsp143I GATC 4 cut(s) 97, 295, 802, 940
BspACI CCGC 2 cut(s) 383, 1282
BspANI GGCC 1 cut(s) 852
BspLI GGNNCC 2 cut(s) 372, 1203
BspPI GGATC 2 cut(s) 105, 948
BsrDI GCAATG 2 cut(s) 499, 1041
BsrI ACTGG 2 cut(s) 905, 922
BssMI GATC 4 cut(s) 97, 295, 802, 940
Bst6I CTCTTC 4 cut(s) 268, 430, 955, 1222
BstAPI GCANNNNNTGC 1 cut(s) 498
BstC8I GCNNGC 2 cut(s) 17, 499
BstDEI CTNAG 3 cut(s) 79, 127, 287
BstF5I GGATG 1 cut(s) 835
BstKTI GATC 4 cut(s) 100, 298, 805, 943
BstMAI GTCTC 2 cut(s) 757, 988
BstMBI GATC 4 cut(s) 97, 295, 802, 940
BstMWI GCNNNNNNNGC 5 cut(s) 25, 461, 498, 1121, 1358
BstNSI RCATGY 1 cut(s) 148
BstSCI CCNGG 1 cut(s) 529
BstSFI CTRYAG 1 cut(s) 619
BstV1I GCAGC 2 cut(s) 829, 937
BstX2I RGATCY 1 cut(s) 97
BstYI RGATCY 1 cut(s) 97
BsuRI GGCC 1 cut(s) 852
BtsCI GGATG 1 cut(s) 835
Cac8I GCNNGC 2 cut(s) 17, 499
Cfr13I GGNCC 1 cut(s) 107
Csp6I GTAC 3 cut(s) 142, 809, 932
CviAII CATG 5 cut(s) 145, 299, 359, 1035, 1153
CviQI GTAC 3 cut(s) 142, 809, 932
DdeI CTNAG 3 cut(s) 79, 127, 287
DpnI GATC 4 cut(s) 99, 297, 804, 942
DpnII GATC 4 cut(s) 97, 295, 802, 940
DraI TTTAAA 1 cut(s) 1384
DrdI GACNNNNNNGTC 1 cut(s) 668
DseDI GACNNNNNNGTC 1 cut(s) 668
Eam1104I CTCTTC 4 cut(s) 268, 430, 955, 1222
EarI CTCTTC 4 cut(s) 268, 430, 955, 1222
Eco47I GGWCC 1 cut(s) 107
Eco57I CTGAAG 2 cut(s) 855, 867
EcoT22I ATGCAT 2 cut(s) 93, 278
FaeI CATG 5 cut(s) 148, 302, 362, 1038, 1156
FalI AAGNNNNNCTT 2 cut(s) 1281, 1313
FatI CATG 5 cut(s) 144, 298, 358, 1034, 1152
FauI CCCGC 1 cut(s) 1289
FauNDI CATATG 1 cut(s) 93
FbaI TGATCA 2 cut(s) 295, 802
FblI GTMKAC 1 cut(s) 65
Fnu4HI GCNGC 2 cut(s) 843, 926
FokI GGATG 1 cut(s) 842
Fsp4HI GCNGC 2 cut(s) 843, 926
FspBI CTAG 4 cut(s) 155, 171, 1179, 1206
GluI GCNGC 2 cut(s) 843, 926
HaeIII GGCC 1 cut(s) 852
HapII CCGG 2 cut(s) 531, 1211
Hin1II CATG 5 cut(s) 148, 302, 362, 1038, 1156
HindIII AAGCTT 3 cut(s) 17, 124, 867
HinfI GANTC 6 cut(s) 516, 669, 764, 1168, 1214, 1370
HpaII CCGG 2 cut(s) 531, 1211
HphI GGTGA 2 cut(s) 196, 977
Hpy166II GTNNAC 3 cut(s) 66, 177, 910
Hpy188I TCNGA 5 cut(s) 208, 874, 955, 1261, 1275
Hpy188III TCNNGA 4 cut(s) 817, 1055, 1331, 1398
Hpy8I GTNNAC 3 cut(s) 66, 177, 910
HpyAV CCTTC 2 cut(s) 842, 1319
HpyCH4V TGCA 8 cut(s) 15, 91, 237, 276, 896, 1046, 1156, 1361
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 461, 498, 1121, 1358
HpyF3I CTNAG 3 cut(s) 79, 127, 287
Hsp92II CATG 5 cut(s) 148, 302, 362, 1038, 1156
Ksp22I TGATCA 2 cut(s) 295, 802
Kzo9I GATC 4 cut(s) 97, 295, 802, 940
LmnI GCTCC 1 cut(s) 376
Lsp1109I GCAGC 2 cut(s) 829, 937
LweI GCATC 4 cut(s) 199, 640, 1060, 1077
MaeI CTAG 4 cut(s) 155, 171, 1179, 1206
MaeIII GTNAC 2 cut(s) 49, 611
MalI GATC 4 cut(s) 99, 297, 804, 942
MboI GATC 4 cut(s) 97, 295, 802, 940
MboII GAAGA 9 cut(s) 30, 255, 447, 869, 942, 1070, 1209, 1386, 1413
MfeI CAATTG 1 cut(s) 768
MflI RGATCY 1 cut(s) 97
MlyI GAGTC 5 cut(s) 525, 678, 758, 1177, 1208
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 6 cut(s) 547, 856, 868, 901, 958, 1114
Mph1103I ATGCAT 2 cut(s) 93, 278
MroXI GAANNNNTTC 1 cut(s) 727
MseI TTAA 8 cut(s) 86, 354, 422, 446, 590, 747, 1137, 1383
MslI CAYNNNNRTG 1 cut(s) 182
MspI CCGG 2 cut(s) 531, 1211
MspR9I CCNGG 1 cut(s) 531
MunI CAATTG 1 cut(s) 768
MwoI GCNNNNNNNGC 5 cut(s) 25, 461, 498, 1121, 1358
NciI CCSGG 1 cut(s) 531
NdeI CATATG 1 cut(s) 93
NdeII GATC 4 cut(s) 97, 295, 802, 940
NlaIII CATG 5 cut(s) 148, 302, 362, 1038, 1156
NlaIV GGNNCC 2 cut(s) 372, 1203
NmeAIII GCCGAG 1 cut(s) 347
NmuCI GTSAC 1 cut(s) 49
NsiI ATGCAT 2 cut(s) 93, 278
NspI RCATGY 1 cut(s) 148
OliI CACNNNNGTG 1 cut(s) 182
PdmI GAANNNNTTC 1 cut(s) 727
PfeI GAWTC 1 cut(s) 1370
PkrI GCNGC 2 cut(s) 844, 927
PleI GAGTC 5 cut(s) 524, 677, 758, 1176, 1208
PpsI GAGTC 5 cut(s) 524, 677, 758, 1176, 1208
PshBI ATTAAT 1 cut(s) 747
PsiI TTATAA 1 cut(s) 714
PspN4I GGNNCC 2 cut(s) 372, 1203
PspPI GGNCC 1 cut(s) 107
PsuI RGATCY 1 cut(s) 97
RsaI GTAC 3 cut(s) 143, 810, 933
RsaNI GTAC 3 cut(s) 142, 809, 932
RseI CAYNNNNRTG 1 cut(s) 182
SaqAI TTAA 8 cut(s) 86, 354, 422, 446, 590, 747, 1137, 1383
SatI GCNGC 2 cut(s) 843, 926
Sau3AI GATC 4 cut(s) 97, 295, 802, 940
Sau96I GGNCC 1 cut(s) 107
SchI GAGTC 5 cut(s) 525, 678, 758, 1177, 1208
ScrFI CCNGG 1 cut(s) 531
SfaNI GCATC 4 cut(s) 199, 640, 1060, 1077
SfcI CTRYAG 1 cut(s) 619
SinI GGWCC 1 cut(s) 107
SmiMI CAYNNNNRTG 1 cut(s) 182
SmlI CTYRAG 2 cut(s) 1305, 1317
SmoI CTYRAG 2 cut(s) 1305, 1317
SsiI CCGC 2 cut(s) 383, 1282
SspMI CTAG 4 cut(s) 155, 171, 1179, 1206
StyD4I CCNGG 1 cut(s) 529
TaqI TCGA 2 cut(s) 159, 816
TatI WGTACW 2 cut(s) 141, 931
TfiI GAWTC 1 cut(s) 1370
Tru1I TTAA 8 cut(s) 86, 354, 422, 446, 590, 747, 1137, 1383
Tru9I TTAA 8 cut(s) 86, 354, 422, 446, 590, 747, 1137, 1383
TseFI GTSAC 1 cut(s) 49
TseI GCWGC 2 cut(s) 842, 925
Tsp45I GTSAC 1 cut(s) 49
TspDTI ATGAA 7 cut(s) 312, 675, 1023, 1030, 1176, 1278, 1356
VpaK11BI GGWCC 1 cut(s) 107
VspI ATTAAT 1 cut(s) 747
XapI RAATTY 3 cut(s) 188, 308, 644
XceI RCATGY 1 cut(s) 148
XmiI GTMKAC 1 cut(s) 65
XmnI GAANNNNTTC 1 cut(s) 727
XspI CTAG 4 cut(s) 155, 171, 1179, 1206
Zsp2I ATGCAT 2 cut(s) 93, 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.