RLG00000032290

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001064
Physical Location & Seq
Forward (+)
13171252 .. 13174663
3412 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032290

Sequence Viewer

Length: 798 bp
ATGGTGTTCTTCTCTCTTATTTGTGATTCTTCGTCGCCATTCTCTTCTAGATTGTTCAAGTTCCTGAATTTCCTCATCTGGGTCTCATTCAATTTTTTCTGTTCTTCAATCTTATGGGCCGGGAAACAATGTTTCGTGCTCAGGGAACTTGTTCAAACTCTAACTAACCGCAAAGAGAAGTTCCGTAAATTGCTTGAATCGTATAAGCCGAGTCTTGTATACTTGCAAGGAGAGCAGCTTGAAAATGATGAAGTTGGTTCTTTTGCCTGGAGAGATACTGATATGCCAACTGCTGAATCTATATCCGACATCTTGGCACATTGCCTACAACTGTTCCCCTTCTTGGTGGAGCATTCTGCGCATGTACTTGGCATTTCTTTTATAACACCGAGTCTCTTGAGACCAGAGGAAGCTGAACTCTTTGCTAAATCTGCACAGAATTCGTATAAGCAATTTCGGGACAAGGCAGCTTCTTCCAGATCAATGACTGCAGGTAAAGATGCAGCTTCAAAGGGTTTAGTTGATGCTATTGGTGGACTTTCTCGGGCTGTTGCAATAGCAAAGCTCAAGGCAAATATACCACAAGACACAGAGGTTACACTAGTGGAACTTGCAAGACCCGCCCCTTCACTGCCAGAACTTTTAATATATAGGTCTGCAACTTGGAGCAGGTGCTTTAAAGGATATAGTGGGGATACTGACCTAAGTCCGAGAAATATTAATAGTCTGAAATCAAGCAAGCAAGATATATTTATATTCCTGAGAGCTGTTTCTGAAAATCAGGTGTTGGTAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

266

Amino Acids

29.7

Weight (kDa)

8.55

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 383
AarI CACCTGC 1 cut(s) 660
Acc16I TGCGCA 1 cut(s) 360
Acc36I ACCTGC 2 cut(s) 482, 660
AccI GTMKAC 1 cut(s) 219
AciI CCGC 2 cut(s) 169, 621
AcsI RAATTY 2 cut(s) 67, 439
AfaI GTAC 1 cut(s) 366
AfiI CCNNNNNNNGG 2 cut(s) 79, 343
AgsI TTSAA 7 cut(s) 58, 91, 108, 155, 197, 242, 510
AhlI ACTAGT 1 cut(s) 601
AjnI CCWGG 1 cut(s) 266
AluBI AGCT 6 cut(s) 238, 413, 470, 506, 565, 767
AluI AGCT 6 cut(s) 238, 413, 470, 506, 565, 767
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 3 cut(s) 88, 394, 398
Ama87I CYCGRG 1 cut(s) 543
AoxI GGCC 1 cut(s) 117
ApeKI GCWGC 3 cut(s) 235, 467, 503
ApoI RAATTY 2 cut(s) 67, 439
AseI ATTAAT 1 cut(s) 720
Asp700I GAANNNNTTC 1 cut(s) 150
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 1 cut(s) 117
AsuC2I CCSGG 1 cut(s) 121
AvaI CYCGRG 1 cut(s) 543
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 3 cut(s) 247, 479, 515
BcgI CGANNNNNNTGC 2 cut(s) 423, 457
BciT130I CCWGG 1 cut(s) 268
BciVI GTATCC 1 cut(s) 688
BcnI CCSGG 1 cut(s) 121
BcoDI GTCTC 3 cut(s) 88, 394, 398
BcuI ACTAGT 1 cut(s) 601
BfaI CTAG 3 cut(s) 48, 602, 796
BfmI CTRYAG 1 cut(s) 489
BfuAI ACCTGC 2 cut(s) 482, 660
BfuI GTATCC 1 cut(s) 688
BisI GCNGC 3 cut(s) 236, 468, 504
BlsI GCNGC 3 cut(s) 237, 469, 505
Bme1390I CCNGG 2 cut(s) 121, 268
BmeT110I CYCGRG 1 cut(s) 543
BmgT120I GGNCC 1 cut(s) 117
BmrFI CCNGG 2 cut(s) 121, 268
BmsI GCATC 2 cut(s) 490, 514
BoxI GACNNNNGTC 1 cut(s) 705
BpmI CTGGAG 1 cut(s) 289
Bpu10I CCTNAGC 1 cut(s) 140
BpuEI CTTGAG 2 cut(s) 418, 551
BpuMI CCSGG 1 cut(s) 121
BsaI GGTCTC 2 cut(s) 88, 394
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 343
Bse3DI GCAATG 1 cut(s) 319
BseBI CCWGG 1 cut(s) 268
BseLI CCNNNNNNNGG 2 cut(s) 79, 343
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 2 cut(s) 154, 752
BseXI GCAGC 3 cut(s) 247, 479, 515
BsgI GTGCAG 1 cut(s) 417
BshFI GGCC 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 543
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 1 cut(s) 473
BslI CCNNNNNNNGG 2 cut(s) 79, 343
BsmAI GTCTC 3 cut(s) 88, 394, 398
BsmFI GGGAC 1 cut(s) 473
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 1 cut(s) 119
Bso31I GGTCTC 2 cut(s) 88, 394
BsoBI CYCGRG 1 cut(s) 543
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 1 cut(s) 479
BspACI CCGC 2 cut(s) 169, 621
BspANI GGCC 1 cut(s) 119
BspCNI CTCAG 2 cut(s) 153, 753
BspMAI CTGCAG 1 cut(s) 493
BspMI ACCTGC 2 cut(s) 482, 660
BspTNI GGTCTC 2 cut(s) 88, 394
BsrDI GCAATG 1 cut(s) 319
BssMI GATC 1 cut(s) 479
BssNAI GTATAC 1 cut(s) 220
Bst1107I GTATAC 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 268
Bst4CI ACNGT 1 cut(s) 333
Bst6I CTCTTC 1 cut(s) 49
BstC8I GCNNGC 1 cut(s) 740
BstDEI CTNAG 3 cut(s) 140, 704, 761
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 1 cut(s) 482
BstMAI GTCTC 3 cut(s) 88, 394, 398
BstMBI GATC 1 cut(s) 479
BstMWI GCNNNNNNNGC 4 cut(s) 232, 358, 431, 620
BstNI CCWGG 1 cut(s) 268
BstNSI RCATGY 1 cut(s) 365
BstPAI GACNNNNGTC 1 cut(s) 705
BstSCI CCNGG 2 cut(s) 119, 266
BstSFI CTRYAG 1 cut(s) 489
BstV1I GCAGC 3 cut(s) 247, 479, 515
BstZ17I GTATAC 1 cut(s) 220
BsuI GTATCC 1 cut(s) 688
BsuRI GGCC 1 cut(s) 119
BtsI GCAGTG 1 cut(s) 629
BtsIMutI CAGTG 1 cut(s) 629
BveI ACCTGC 2 cut(s) 482, 660
Cac8I GCNNGC 1 cut(s) 740
CfoI GCGC 1 cut(s) 361
Cfr13I GGNCC 1 cut(s) 117
Csp6I GTAC 1 cut(s) 365
CviAII CATG 1 cut(s) 362
CviJI RGCY 9 cut(s) 119, 208, 238, 413, 470, 506, 548, 565, 767
CviKI_1 RGCY 9 cut(s) 119, 208, 238, 413, 470, 506, 548, 565, 767
CviQI GTAC 1 cut(s) 365
DdeI CTNAG 3 cut(s) 140, 704, 761
DpnI GATC 1 cut(s) 481
DpnII GATC 1 cut(s) 479
DraI TTTAAA 1 cut(s) 679
Eam1104I CTCTTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 49
Eco31I GGTCTC 2 cut(s) 88, 394
Eco88I CYCGRG 1 cut(s) 543
EcoRI GAATTC 1 cut(s) 439
EcoRII CCWGG 1 cut(s) 266
FaeI CATG 1 cut(s) 365
FaqI GGGAC 1 cut(s) 473
FatI CATG 1 cut(s) 361
FauI CCCGC 1 cut(s) 628
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 3 cut(s) 236, 468, 504
Fsp4HI GCNGC 3 cut(s) 236, 468, 504
FspBI CTAG 3 cut(s) 48, 602, 796
FspI TGCGCA 1 cut(s) 360
GlaI GCGC 1 cut(s) 360
GluI GCNGC 3 cut(s) 236, 468, 504
GsuI CTGGAG 1 cut(s) 289
HaeIII GGCC 1 cut(s) 119
HapII CCGG 1 cut(s) 120
HhaI GCGC 1 cut(s) 361
Hin1II CATG 1 cut(s) 365
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HinfI GANTC 5 cut(s) 26, 197, 211, 296, 391
HpaII CCGG 1 cut(s) 120
Hpy166II GTNNAC 2 cut(s) 220, 536
Hpy188I TCNGA 4 cut(s) 307, 711, 729, 775
Hpy188III TCNNGA 6 cut(s) 48, 64, 397, 458, 477, 760
Hpy8I GTNNAC 2 cut(s) 220, 536
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 2 cut(s) 349, 636
HpyCH4III ACNGT 1 cut(s) 333
HpyCH4V TGCA 7 cut(s) 226, 434, 491, 503, 554, 614, 659
HpyF10VI GCNNNNNNNGC 4 cut(s) 232, 358, 431, 620
HpyF3I CTNAG 3 cut(s) 140, 704, 761
Hsp92II CATG 1 cut(s) 365
HspAI GCGC 1 cut(s) 359
Kzo9I GATC 1 cut(s) 479
LmnI GCTCC 2 cut(s) 349, 666
Lsp1109I GCAGC 3 cut(s) 247, 479, 515
LweI GCATC 2 cut(s) 490, 514
MaeI CTAG 3 cut(s) 48, 602, 796
MaeIII GTNAC 1 cut(s) 595
MalI GATC 1 cut(s) 481
MboI GATC 1 cut(s) 479
MboII GAAGA 4 cut(s) 21, 36, 96, 465
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 5 cut(s) 67, 91, 188, 439, 452
MlyI GAGTC 2 cut(s) 220, 400
MmeI TCCRAC 1 cut(s) 330
MnlI CCTC 3 cut(s) 83, 400, 586
MroXI GAANNNNTTC 1 cut(s) 150
MseI TTAA 3 cut(s) 644, 678, 720
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 2 cut(s) 121, 268
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 1 cut(s) 268
MwoI GCNNNNNNNGC 4 cut(s) 232, 358, 431, 620
NciI CCSGG 1 cut(s) 121
NdeII GATC 1 cut(s) 479
NlaIII CATG 1 cut(s) 365
NmeAIII GCCGAG 1 cut(s) 234
NsbI TGCGCA 1 cut(s) 360
NspI RCATGY 1 cut(s) 365
PaqCI CACCTGC 1 cut(s) 660
PcsI WCGNNNNNNNCGW 1 cut(s) 206
PctI GAATGC 1 cut(s) 352
PdmI GAANNNNTTC 1 cut(s) 150
PfeI GAWTC 3 cut(s) 26, 197, 296
PkrI GCNGC 3 cut(s) 237, 469, 505
PleI GAGTC 2 cut(s) 219, 399
PpsI GAGTC 2 cut(s) 219, 399
PshAI GACNNNNGTC 1 cut(s) 705
PshBI ATTAAT 1 cut(s) 720
PsiI TTATAA 1 cut(s) 383
Psp6I CCWGG 1 cut(s) 266
PspGI CCWGG 1 cut(s) 266
PspPI GGNCC 1 cut(s) 117
PstI CTGCAG 1 cut(s) 493
RsaI GTAC 1 cut(s) 366
RsaNI GTAC 1 cut(s) 365
SaqAI TTAA 3 cut(s) 644, 678, 720
SatI GCNGC 3 cut(s) 236, 468, 504
Sau3AI GATC 1 cut(s) 479
Sau96I GGNCC 1 cut(s) 117
SchI GAGTC 2 cut(s) 220, 400
ScrFI CCNGG 2 cut(s) 121, 268
SduI GDGCHC 1 cut(s) 141
SfaNI GCATC 2 cut(s) 490, 514
SfcI CTRYAG 1 cut(s) 489
SmlI CTYRAG 2 cut(s) 397, 566
SmoI CTYRAG 2 cut(s) 397, 566
SpeI ACTAGT 1 cut(s) 601
Sse9I AATT 5 cut(s) 67, 91, 188, 439, 452
SsiI CCGC 2 cut(s) 169, 621
SspI AATATT 1 cut(s) 718
SspMI CTAG 3 cut(s) 48, 602, 796
StyD4I CCNGG 2 cut(s) 119, 266
TaaI ACNGT 1 cut(s) 333
TasI AATT 5 cut(s) 67, 91, 188, 439, 452
TatI WGTACW 1 cut(s) 364
TfiI GAWTC 3 cut(s) 26, 197, 296
Tru1I TTAA 3 cut(s) 644, 678, 720
Tru9I TTAA 3 cut(s) 644, 678, 720
TscAI CASTG 1 cut(s) 636
TseI GCWGC 3 cut(s) 235, 467, 503
TspDTI ATGAA 1 cut(s) 264
TspGWI ACGGA 1 cut(s) 173
TspRI CASTG 1 cut(s) 636
VspI ATTAAT 1 cut(s) 720
XapI RAATTY 2 cut(s) 67, 439
XbaI TCTAGA 1 cut(s) 47
XceI RCATGY 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 150
XspI CTAG 3 cut(s) 48, 602, 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.