Rorug01G0280100

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001064
Physical Location & Seq
Reverse (-)
39323764 .. 39329315
5552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0280100.1

Sequence Viewer

Length: 5202 bp
ATGGAGTCGGGAAGGTGGCTCAGACATGCTTTTGCAATATCAATTGTGCTTCTTCTGCATATCAACGGTTCCTTATCACTCGGCAACCACCATGCTAACTTGACGACGAGGTGCCTTGAGAGGGAGAGGGGTGCACTCCTTGCTATCAAAGGAGACCTGATGGATGAATACAACCTGCTCTCTTCTTGGGGAAGCGAAGCCAAAAAGCAAGACTGTTGCCGATGGGAAAGAGTCCACTGCGATCACCAGACTGGCCATGTTACTCAACTTCATCTTGGAGGACTGGAGGTGATCTCTTCACTAGGTGAGTTTATGACTTACAAATATTTTAAAGGAATAGTTAGTCCTAAACTCATTGAGTTGCAGCATTTGGAATATTTGGACCTCAGTCACAATTTCTTCAATGCCAACCAAATTCCAGCGCTCATTTGTTCTCTGTCCAATTTAAGACACCTAGATCTCCATTACAATGACCTCACTGGGAGCAAAATTCTGGAGCTCATTAGTAATCTGACCAATTTAAGATATCTAGATCTCTCAAGGTATGCCTTTGGAGGTGAAACTCCATATCATCAGCTTGGAAACCTTACTCATTTGCAGTATCTCAATCTCGGGTATAATGACATCACTCATGGTGTAGAAAACCTCCATTGGCTCCCACATCTTTCTTCTTTAAAATACCTGGACCTGAGTTGGGCCGATATAAGTAAGGCTTCTGATTGGTTGGAAACAATAAACAAGCTCCCCAATTTAAGAAACTTAACCTTGAGATTCTGTAGTCTTCCTCCTCCAACTCTTTCCACTCTTTCTCACATAAATTCTTCCAATTCTCTTGTTAGTGTTGATTTGTCTGGAAATCCTGATGTCATTTCTTCAATATTCAAATGGTTGTGCAATTTCAATACCACCCTTGTTCATCTTGACCTCTCTTCCAACCAACTAAGTGGTTTGATTCCGGATGCTTTTGCAAACATGAGCTCACTTGCATATCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGATTCCCGATGTTTTCAGAAACATGGGCTCACTTGAACATCTATCCCTCTCTTCCAACCAACTAAGTGGTTTGATTCCGGATGCTTTTGCAAACATGAGCTCACTTGCATATCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGATTCCCGATGTTTTCAGAAACATGGGCTCACTTGAACATCTATCCCTCTCTTCCAACCAATTTAGTGGTTTGATTCCGGATGCTTTTGCAAACATTGGCTCACTTGAACATCTATCCCTCTCTTCCAACCAATTCAGTGGTTTGATTCTGGATGCTTTTGCAAACATGGGCTCACTTGCATATCTTGACCTCTCTTCCAACCAATTCAGTGGTTTGATTCCCGATGTTTTCAGAAACATGGGCTCACTTGAACATCTATCCCTCTCTTCCAACCAATTAAGTGGTTTGATTCACGATGCTTTCGCAAATATGAGCTCACTTGAAAATCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGACTCCCGATGTTTTCAGAAACATGGGCTCGCTTAAATATCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGACTCCTAATGCTTTTGCAAACATGAGCTCACTTGCATATCTCAGTCTCTCTTCCAACCAATTAAGTGGTTTGATTCCGAATGCTTTCGCAAACATGAGCTCCCTTGCATATCTCAGTCTCTCTTCCAACCAATTAAGTGGTTTGACTCCCAATGTTTTCAGAAACATGGGCTCGCTTGAATATTTATACCTCTCTTCCAACCAATTAAGTGGTTTGATTCCCAATGTTTTCAGAAACATGGGCTCGCTTGAATATCTATACCTCTCTTCCAACCAATTAAGTGGTTTGACTCCTAATGCTTTTGCAAACATGAGCTCACTTGCATATCTCAGTCTCTCTTCCAACCAATTAAGTGGTTTGAGTCCCGATGCTTTCGCAAACATGAGGTCACTTGCACATCTTTACCTCTCTTCCAACCAATTAAGTGGTTTGATTCCCGATGCTTTCAGAAACATGAGCTCACTTGAATATCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGATTCCCGATGCTTTCAGAAACATGAGCTCACTTGAATATCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGATTCCCGATGCTTTCAGAAACATGGGCTCACTTGCATATCTCATTCTCTCTTCCAACCAATTAAGTGGTTTGATTCCCGATGCTTTCAGAAACATGGGCTCACTTGCATATCTCATTCTCTCTTCCAACCAATTAAGTGGTTTGATTCCCGATGCTTTCAGAAACATGAGCTCACTTGAATATCTCAGTCTCTCTTCCAACCAATTAAGTGGCTTGATTCCCGATGCTTTCAGAAACATGAGCTCACTTGAATATCTCAGTCTCTCTTCCAACCAATTAAGTGGCTTGATTCCCGATGCTTTCAGAAACATGGGCTCACTTGCATCTCTCGGTCTCTCTTCCAACCAATTAAGTGGCTTGATTCCCGATGCTTTCAGAAACATGGGCTCACTTGCATCTCTCAGTCTCTCTTCCAACCAATTAAGTGGTTTGAGTCCCAATGCTTTTGCAAACATGAGGTCACTTGCACGTCTTTACCTCTCTTCCAACCAATTAAGTGGTTTCATTCCCGATGCTTTCAGAAACATGAGCTCACTTGAATATCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGATTCCCGATGCTTTCAGAAACATGGGCTCACTTGAACATCTATCCCTCTCTTCCAACCAATTTAGTGGTTTGATTCTGAATGCTTTCGCAAACATGAGCTCACTTGCATATCTTAACCTCTCTTCCAACCAATTAAGTGGATTGATTCCTAATGCTTTCGCAAATATGCGCTCACTTGCACGTCTTGATATCTCTTATAATCCACTCGAAGGAGGGATCCCAGCATTCATTGGCCAGTTATGTAGTTTGCGACATGTGTCAGTTTTAGGGTGTAATCTGACTAGTGGACAACTTTCTGAGTTTGTTCAAGGATGTTCGACATGTGTTCAAAACTCATTACAGAGTTTTGATCTTTCCAATAATCATCTTGCTGGGTCACTTCCTAATCTTACAAGCTTTTCATCATTGGAGGTATTAACTCTCTCCAAAAATAAGTTGAGTGTTCTGGATAGTATTGGAAATTTTTCTTCTCTGAAACAATTATATGTTGATCAAAATCAATTACGATGGATGATACCGGAAAGTATTGGGAAACTCTCCAGTTTGTATGTCTTGGATCTCTCTGGAAATCTTCTGAGCGGAAGAATTCCAAAAGGTATTGGGCAACTCTCATCATTGGAACGTTTAGTTCTTTCTGGAAATCAATTAAGCAGAAGCATACCTGAAACTATTGGGCAACTATCATCTTTGCAAAGTTTAGATCTCTCTAGAAATCTGTTAAGCGGAAGAATACCGCACGGTATTGGACAGTTATCGTGGTTGGGCTATGTAAATCTTAGTATAAATCATTTGGAAGGGAATATTTCAGAATATCATTTCTCAAAACTCTTTCATTTATATGAGTTGGATTTATCCTATAACTCATTGTTAGAATTCAATGTCACTTCTGATTGGATTCCTCCTTTTCAATTGAATTACCTAAGTTTGAGGTATTGTAAGGTGGGGCCGGGTTTCCCAAAATGGATTCGAACCCAAAAAGGTCTTTCCAACCTTGATATGTCTTATGGTGACATTTCAGATGTCTTTCCAAGTTGGTTCTGGAGTCTATTTGGCGACGCTCCGACTATAGATTTGTCTAACAATCAAATTAGAGGAACCTTAGGGAGTTCAAAGGTTGTATCATTCAGCGGAGTTGAAGTTCGGTTTAGTTCGAACCAATTGGAAGGTCCAATACCCTCATTTCTATCAAAAGCGTCATATTTGGATCTCTCTGATAATAAATTTTCAGAAATGACTTCCTTCTTGTGTTCTTCGAATGTTAAAATTTCCAAATTTCTTGATCTCTCCAGTAACCATATTCCTGGAGAAGTTCCAGATTGCTGGAGACATTGGGTCAATCTAGAACTTCTCGATTTGAGTAACAATTCTTTTTCTGGGAAAATCCCACCCAATATAGGATGTTTACTTCGAATCAAAACATTGAAATTGAGGAGCAACAAACTTGTGGGAGAATTGCCTTCATCCTTGAAGAACTGTACAAGCTTAAATGTTATTGATCTTGGGAACAATAAGCTTTCAAGTTCAGTGCCTGAATGGTTAGGGGTCAGTCTTCCAAATTTGGTTATTCTTATGCTTCAATCTAATCAGCTTAGTGGAAGTTTGCCTTCACAATTATGCCATCTAGCACGCCTTCAAATTTTGGATGTCTCTGTGAATCAAATCTCTGGATCTATACCAAAATGCCTCGATAATTTGACTTCTTTGGCGCAAGAAGGAAATTCAAATCTAACCATCAGACATTCTTTTAATACATCTGAGGCCGAACCACCTATGAGTTCTCTTTATTATGAGGATGATGCAACCTTCATGTGGAAGAGAACATTGTCTACATACAAAAACACTTTGGGGCTAGTCAAGAGCATTGATCTCTCATGCAACAGATTAACTGGGGAGATTCCAAGTGAAATCACTCATCTTGTTGGGCTCGTTTCTCTGAACCTTTCAAGGAACAATTTGACTGGACAAATACCTCCAGAGATCGGAAAGTTGAAGTCACTAGATTCTCTTGATTTGTCTAGAAACAAGATTGGTGGCAGGATTCCGACAAGCCTTGCTCGGATTGATAGACTTGGTGACATGGATTTATCATATAACAACTTGTCTGGTGAAATTCCAACTGGCACTCAGCTCCAGAGCTTTGATCCATCCTTTTATGCCGGAAATCCTCAATTGTGTGGACGTCCACTTGAAGCTTGTAATCCAGAGGGAACTGGTCGGCCAAATGTCTCGAGTGATCAAGCAGATCCAGATGAGGTCATCACACCGGGCTTTTACATAAGTCTGGGACTTGGATTTGCTGTTGGATTCTGGGTTGTCTGTGGGAGCTTGATATTCAAGCGGTCATGGAGATATGCATACTACAACTTCTTGAATGCTTTAAATGATTGGCTTTATGTGAGGGTAGCTTTGATCAGACGGCAATTAAAGGATATGCTTAGTGGACAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000086 GO:0000278 GO:0003674 GO:0003682 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006807 GO:0006996 GO:0007049 GO:0007623 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008214 GO:0008276 GO:0008757 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010452 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016278 GO:0016279 GO:0016491 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016705 GO:0016706 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032259 GO:0032451 GO:0032452 GO:0034968 GO:0036211 GO:0042054 GO:0042752 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044839 GO:0045893 GO:0045935 GO:0046975 GO:0048511 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051213 GO:0051252 GO:0051254 GO:0051276 GO:0051864 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070076 GO:0070544 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:0140110 GO:1901564 GO:1902680 GO:1903047 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_8

Protein Analysis

1733

Amino Acids

189.95

Weight (kDa)

5.69

Isoelectric Point (pI)

34.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 41 - 81 2.6e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 117 - 337 2e-12 Leucine-rich repeat region
LRR_8 PF13855 277 - 338 9.8e-09 Leucine rich repeat
LRR_8 PF13855 303 - 362 7.3e-15 Leucine rich repeat
LRR_8 PF13855 331 - 386 6.4e-14 Leucine rich repeat
LRR_5 PF13306 345 - 456 4.8e-06 BspA type Leucine rich repeat region (6 copies)
LRR_8 PF13855 351 - 410 2.9e-14 Leucine rich repeat
LRR_8 PF13855 375 - 434 2.4e-13 Leucine rich repeat
LRR_5 PF13306 392 - 504 2.5e-06 BspA type Leucine rich repeat region (6 copies)
LRR_8 PF13855 406 - 458 4e-09 Leucine rich repeat
LRR_8 PF13855 440 - 482 6.4e-09 Leucine rich repeat
LRR_8 PF13855 447 - 506 3.7e-13 Leucine rich repeat
LRR_5 PF13306 464 - 591 2.8e-11 BspA type Leucine rich repeat region (6 copies)
LRR_8 PF13855 477 - 530 2.4e-12 Leucine rich repeat
LRR_8 PF13855 495 - 554 1.2e-16 Leucine rich repeat
LRR_8 PF13855 519 - 578 4.2e-15 Leucine rich repeat
LRR_8 PF13855 543 - 602 1.5e-14 Leucine rich repeat
LRR_5 PF13306 560 - 688 6.4e-10 BspA type Leucine rich repeat region (6 copies)
LRR_8 PF13855 567 - 626 5.3e-15 Leucine rich repeat
LRR_8 PF13855 591 - 650 9.1e-15 Leucine rich repeat
LRR_5 PF13306 608 - 720 5.8e-09 BspA type Leucine rich repeat region (6 copies)
LRR_8 PF13855 615 - 674 5.3e-17 Leucine rich repeat
LRR_8 PF13855 639 - 698 8.8e-17 Leucine rich repeat
LRR_8 PF13855 667 - 722 5.5e-16 Leucine rich repeat
LRR_4 PF12799 687 - 725 8.7e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 687 - 746 3.9e-16 Leucine rich repeat
LRR_5 PF13306 704 - 816 7.4e-07 BspA type Leucine rich repeat region (6 copies)
LRR_8 PF13855 735 - 794 2.1e-14 Leucine rich repeat
LRR_5 PF13306 776 - 904 2.7e-09 BspA type Leucine rich repeat region (6 copies)
LRR_8 PF13855 789 - 842 1.5e-12 Leucine rich repeat
LRR_8 PF13855 831 - 890 1.1e-14 Leucine rich repeat
LRR_5 PF13306 870 - 982 7.4e-08 BspA type Leucine rich repeat region (6 copies)
LRR_8 PF13855 883 - 938 5.9e-14 Leucine rich repeat
LRR_8 PF13855 945 - 986 2.2e-09 Leucine rich repeat
LRR_14 PF23598 1042 - 1250 1e-20 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2949
AatII GACGTC 1 cut(s) 4933
Acc36I ACCTGC 1 cut(s) 183
AccB1I GGYRCC 1 cut(s) 111
AccBSI CCGCTC 1 cut(s) 3330
AccI GTMKAC 1 cut(s) 4577
AccIII TCCGGA 3 cut(s) 955, 1099, 1243
AciI CCGC 5 cut(s) 3330, 3474, 3485, 3879, 5089
AclI AACGTT 1 cut(s) 3373
AclWI GGATC 7 cut(s) 2962, 2975, 3315, 3963, 4426, 4886, 4988
AcoI YGGCCR 3 cut(s) 253, 2983, 4967
AcyI GRCGYC 1 cut(s) 4930
AdeI CACNNNGTG 1 cut(s) 305
AfaI GTAC 1 cut(s) 4228
AfeI AGCGCT 1 cut(s) 423
AfiI CCNNNNNNNGG 6 cut(s) 121, 694, 2960, 4145, 4560, 4730
AflIII ACRYGT 2 cut(s) 3004, 3071
AhdI GACNNNNNGTC 1 cut(s) 4082
AhlI ACTAGT 1 cut(s) 3032
AjiI CACGTC 2 cut(s) 2645, 2933
AjnI CCWGG 2 cut(s) 681, 4051
AloI GAACNNNNNNTCC 2 cut(s) 3873, 3905
Alw44I GTGCAC 1 cut(s) 132
AlwI GGATC 7 cut(s) 2962, 2975, 3315, 3963, 4426, 4886, 4988
AlwNI CAGNNNCTG 1 cut(s) 4280
Ama87I CYCGRG 2 cut(s) 611, 4978
Aor13HI TCCGGA 3 cut(s) 955, 1099, 1243
Aor51HI AGCGCT 1 cut(s) 423
AoxI GGCC 6 cut(s) 253, 696, 2983, 3695, 4509, 4967
ApaLI GTGCAC 1 cut(s) 132
ApeKI GCWGC 1 cut(s) 364
ArsI GACNNNNNNTTYG 6 cut(s) 1970, 2002, 2618, 2650, 3805, 3837
Asp700I GAANNNNTTC 3 cut(s) 1682, 2834, 3214
AspLEI GCGC 3 cut(s) 424, 2922, 4459
AspS9I GGNCC 5 cut(s) 382, 685, 696, 3695, 3917
AsuC2I CCSGG 2 cut(s) 3699, 5016
AsuHPI GGTGA 7 cut(s) 236, 301, 317, 569, 3770, 4835, 4868
AsuII TTCGAA 4 cut(s) 3718, 3902, 4004, 4159
AvaI CYCGRG 2 cut(s) 611, 4978
AvaII GGWCC 3 cut(s) 382, 685, 3917
AxyI CCTNAGG 1 cut(s) 3850
BaeGI GKGCMC 1 cut(s) 136
BalI TGGCCA 2 cut(s) 255, 2985
BamHI GGATCC 1 cut(s) 2967
BanI GGYRCC 1 cut(s) 111
BarI GAAGNNNNNNTAC 2 cut(s) 3906, 3938
BbsI GAAGAC 2 cut(s) 773, 4292
BbvI GCAGC 1 cut(s) 376
BccI CCATC 6 cut(s) 154, 216, 3252, 4377, 4490, 4903
BceAI ACGGC 1 cut(s) 5183
BciT130I CCWGG 2 cut(s) 683, 4053
BclI TGATCA 3 cut(s) 3241, 4984, 5160
BcnI CCSGG 2 cut(s) 3699, 5016
BcuI ACTAGT 1 cut(s) 3032
BfmI CTRYAG 2 cut(s) 775, 3816
BfoI RGCGCY 1 cut(s) 425
BfuAI ACCTGC 1 cut(s) 183
BglII AGATCT 3 cut(s) 457, 532, 3451
BisI GCNGC 1 cut(s) 365
BlsI GCNGC 1 cut(s) 366
Bme1390I CCNGG 4 cut(s) 683, 3699, 4053, 5016
Bme18I GGWCC 3 cut(s) 382, 685, 3917
BmeRI GACNNNNNGTC 1 cut(s) 4082
BmeT110I CYCGRG 2 cut(s) 611, 4978
BmgBI CACGTC 2 cut(s) 2645, 2933
BmgT120I GGNCC 5 cut(s) 382, 685, 696, 3695, 3917
BmiI GGNNCC 6 cut(s) 70, 113, 656, 2969, 3696, 3847
BmrFI CCNGG 4 cut(s) 683, 3699, 4053, 5016
BmrI ACTGGG 2 cut(s) 489, 4647
BmuI ACTGGG 2 cut(s) 489, 4647
BoxI GACNNNNGTC 2 cut(s) 387, 3007
BpiI GAAGAC 2 cut(s) 773, 4292
BplI GAGNNNNNCTC 2 cut(s) 278, 310
BpmI CTGGAG 9 cut(s) 305, 515, 3274, 3811, 4021, 4074, 4093, 4707, 4865
Bpu14I TTCGAA 4 cut(s) 3718, 3902, 4004, 4159
BpuEI CTTGAG 3 cut(s) 137, 523, 787
BpuMI CCSGG 2 cut(s) 3699, 5016
BsaBI GATNNNNATC 1 cut(s) 3246
BsaHI GRCGYC 1 cut(s) 4930
BsaI GGTCTC 2 cut(s) 147, 2513
BsaWI WCCGGW 4 cut(s) 955, 1099, 1243, 3268
BsaXI ACNNNNNCTCC 4 cut(s) 1682, 1712, 3873, 3903
Bsc4I CCNNNNNNNGG 6 cut(s) 121, 694, 2960, 4145, 4560, 4730
Bse21I CCTNAGG 1 cut(s) 3850
Bse8I GATNNNNATC 1 cut(s) 3246
BseAI TCCGGA 3 cut(s) 955, 1099, 1243
BseBI CCWGG 2 cut(s) 683, 4053
BseJI GATNNNNATC 1 cut(s) 3246
BseLI CCNNNNNNNGG 6 cut(s) 121, 694, 2960, 4145, 4560, 4730
BseRI GAGGAG 2 cut(s) 777, 4195
BseSI GKGCMC 1 cut(s) 136
BseXI GCAGC 1 cut(s) 376
BseYI CCCAGC 2 cut(s) 2971, 3122
BshFI GGCC 6 cut(s) 255, 698, 2985, 3697, 4511, 4969
BshNI GGYRCC 1 cut(s) 111
BsiHKCI CYCGRG 2 cut(s) 611, 4978
BsiSI CCGG 7 cut(s) 956, 1100, 1244, 3269, 3698, 4908, 5015
BslFI GGGAC 3 cut(s) 1947, 2595, 5049
BslI CCNNNNNNNGG 6 cut(s) 121, 694, 2960, 4145, 4560, 4730
BsmFI GGGAC 3 cut(s) 1947, 2595, 5049
BsmI GAATGC 4 cut(s) 1684, 2836, 2975, 5128
BsnI GGCC 6 cut(s) 255, 698, 2985, 3697, 4511, 4969
Bso31I GGTCTC 2 cut(s) 147, 2513
BsoBI CYCGRG 2 cut(s) 611, 4978
Bsp119I TTCGAA 4 cut(s) 3718, 3902, 4004, 4159
Bsp13I TCCGGA 3 cut(s) 955, 1099, 1243
Bsp1407I TGTACA 1 cut(s) 4226
BspACI CCGC 5 cut(s) 3330, 3474, 3485, 3879, 5089
BspANI GGCC 6 cut(s) 255, 698, 2985, 3697, 4511, 4969
BspEI TCCGGA 3 cut(s) 955, 1099, 1243
BspLI GGNNCC 6 cut(s) 70, 113, 656, 2969, 3696, 3847
BspMI ACCTGC 1 cut(s) 183
BspPI GGATC 7 cut(s) 2962, 2975, 3315, 3963, 4426, 4886, 4988
BspT104I TTCGAA 4 cut(s) 3718, 3902, 4004, 4159
BspT107I GGYRCC 1 cut(s) 111
BspTNI GGTCTC 2 cut(s) 147, 2513
BsrBI CCGCTC 1 cut(s) 3330
BsrGI TGTACA 1 cut(s) 4226
BssNI GRCGYC 1 cut(s) 4930
Bst2UI CCWGG 2 cut(s) 683, 4053
Bst4CI ACNGT 5 cut(s) 68, 215, 3491, 3501, 4226
BstACI GRCGYC 1 cut(s) 4930
BstAPI GCANNNNNTGC 1 cut(s) 140
BstAUI TGTACA 1 cut(s) 4226
BstBI TTCGAA 4 cut(s) 3718, 3902, 4004, 4159
BstC8I GCNNGC 4 cut(s) 1556, 1772, 1844, 4378
BstH2I RGCGCY 1 cut(s) 425
BstHHI GCGC 3 cut(s) 424, 2922, 4459
BstMWI GCNNNNNNNGC 2 cut(s) 55, 140
BstNI CCWGG 2 cut(s) 683, 4053
BstNSI RCATGY 3 cut(s) 29, 3008, 3075
BstPAI GACNNNNGTC 2 cut(s) 387, 3007
BstSCI CCNGG 4 cut(s) 681, 3697, 4051, 5014
BstSFI CTRYAG 2 cut(s) 775, 3816
BstSLI GKGCMC 1 cut(s) 136
BstV1I GCAGC 1 cut(s) 376
BstV2I GAAGAC 2 cut(s) 773, 4292
BstX2I RGATCY 8 cut(s) 457, 532, 2967, 3307, 3451, 3955, 4418, 4993
BstYI RGATCY 8 cut(s) 457, 532, 2967, 3307, 3451, 3955, 4418, 4993
Bsu36I CCTNAGG 1 cut(s) 3850
BsuRI GGCC 6 cut(s) 255, 698, 2985, 3697, 4511, 4969
BtrI CACGTC 2 cut(s) 2645, 2933
BtsI GCAGTG 1 cut(s) 235
BtsIMutI CAGTG 5 cut(s) 235, 477, 1309, 1381, 4281
BveI ACCTGC 1 cut(s) 183
Cac8I GCNNGC 4 cut(s) 1556, 1772, 1844, 4378
CaiI CAGNNNCTG 1 cut(s) 4280
CfoI GCGC 3 cut(s) 424, 2922, 4459
Cfr13I GGNCC 5 cut(s) 382, 685, 696, 3695, 3917
CseI GACGC 2 cut(s) 3815, 3933
Csp6I GTAC 1 cut(s) 4227
CspCI CAANNNNNGTGG 2 cut(s) 4762, 4797
CviQI GTAC 1 cut(s) 4227
DraI TTTAAA 3 cut(s) 331, 675, 5130
DraIII CACNNNGTG 1 cut(s) 305
DriI GACNNNNNGTC 1 cut(s) 4082
EaeI YGGCCR 3 cut(s) 253, 2983, 4967
Eam1105I GACNNNNNGTC 1 cut(s) 4082
Eco31I GGTCTC 2 cut(s) 147, 2513
Eco32I GATATC 2 cut(s) 527, 2941
Eco47I GGWCC 3 cut(s) 382, 685, 3917
Eco47III AGCGCT 1 cut(s) 423
Eco81I CCTNAGG 1 cut(s) 3850
Eco88I CYCGRG 2 cut(s) 611, 4978
EcoRI GAATTC 2 cut(s) 3336, 3623
EcoRII CCWGG 2 cut(s) 681, 4051
EcoRV GATATC 2 cut(s) 527, 2941
EcoT22I ATGCAT 1 cut(s) 5107
FalI AAGNNNNNCTT 4 cut(s) 697, 729, 4339, 4371
FaqI GGGAC 3 cut(s) 1947, 2595, 5049
FbaI TGATCA 3 cut(s) 3241, 4984, 5160
FblI GTMKAC 1 cut(s) 4577
Fnu4HI GCNGC 1 cut(s) 365
Fsp4HI GCNGC 1 cut(s) 365
GlaI GCGC 3 cut(s) 423, 2921, 4458
GluI GCNGC 1 cut(s) 365
GsaI CCCAGC 2 cut(s) 2975, 3126
GsuI CTGGAG 9 cut(s) 305, 515, 3274, 3811, 4021, 4074, 4093, 4707, 4865
HaeII RGCGCY 1 cut(s) 425
HaeIII GGCC 6 cut(s) 255, 698, 2985, 3697, 4511, 4969
HapII CCGG 7 cut(s) 956, 1100, 1244, 3269, 3698, 4908, 5015
HgaI GACGC 2 cut(s) 3815, 3933
HhaI GCGC 3 cut(s) 424, 2922, 4459
Hin1I GRCGYC 1 cut(s) 4930
Hin6I GCGC 3 cut(s) 422, 2920, 4457
HinP1I GCGC 3 cut(s) 422, 2920, 4457
HindIII AAGCTT 4 cut(s) 3145, 4231, 4262, 4941
HpaII CCGG 7 cut(s) 956, 1100, 1244, 3269, 3698, 4908, 5015
HphI GGTGA 7 cut(s) 236, 301, 317, 569, 3770, 4835, 4868
Hpy166II GTNNAC 8 cut(s) 134, 235, 3038, 4154, 4578, 4928, 4934, 5192
Hpy8I GTNNAC 8 cut(s) 134, 235, 3038, 4154, 4578, 4928, 4934, 5192
Hpy99I CGWCG 2 cut(s) 109, 3809
HpyCH4III ACNGT 5 cut(s) 68, 215, 3491, 3501, 4226
HpyCH4IV ACGT 4 cut(s) 2644, 2932, 3373, 4930
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 140
HpySE526I ACGT 4 cut(s) 2644, 2932, 3373, 4930
Hsp92I GRCGYC 1 cut(s) 4930
HspAI GCGC 3 cut(s) 422, 2920, 4457
Kpn2I TCCGGA 3 cut(s) 955, 1099, 1243
Ksp22I TGATCA 3 cut(s) 3241, 4984, 5160
LmnI GCTCC 9 cut(s) 483, 496, 660, 747, 1703, 3814, 4182, 4884, 5073
Lsp1109I GCAGC 1 cut(s) 376
MaeII ACGT 4 cut(s) 2644, 2932, 3373, 4930
MbiI CCGCTC 1 cut(s) 3330
MfeI CAATTG 4 cut(s) 42, 3659, 3908, 4919
MflI RGATCY 8 cut(s) 457, 532, 2967, 3307, 3451, 3955, 4418, 4993
MlsI TGGCCA 2 cut(s) 255, 2985
MluNI TGGCCA 2 cut(s) 255, 2985
MlyI GAGTC 9 cut(s) 14, 240, 1522, 1594, 1738, 1882, 1969, 2617, 3802
Mox20I TGGCCA 2 cut(s) 255, 2985
Mph1103I ATGCAT 1 cut(s) 5107
MroI TCCGGA 3 cut(s) 955, 1099, 1243
MroXI GAANNNNTTC 3 cut(s) 1682, 2834, 3214
MscI TGGCCA 2 cut(s) 255, 2985
MslI CAYNNNNRTG 3 cut(s) 468, 3588, 4363
Msp20I TGGCCA 2 cut(s) 255, 2985
MspA1I CMGCKG 1 cut(s) 3879
MspI CCGG 7 cut(s) 956, 1100, 1244, 3269, 3698, 4908, 5015
MspR9I CCNGG 4 cut(s) 683, 3699, 4053, 5016
MunI CAATTG 4 cut(s) 42, 3659, 3908, 4919
Mva1269I GAATGC 4 cut(s) 1684, 2836, 2975, 5128
MvaI CCWGG 2 cut(s) 683, 4053
MwoI GCNNNNNNNGC 2 cut(s) 55, 140
NciI CCSGG 2 cut(s) 3699, 5016
NlaIV GGNNCC 6 cut(s) 70, 113, 656, 2969, 3696, 3847
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 8 cut(s) 389, 1986, 2634, 3126, 3631, 3758, 4743, 4823
NsiI ATGCAT 1 cut(s) 5107
NspI RCATGY 3 cut(s) 29, 3008, 3075
NspV TTCGAA 4 cut(s) 3718, 3902, 4004, 4159
PaeR7I CTCGAG 1 cut(s) 4978
PciI ACATGT 2 cut(s) 3004, 3071
PctI GAATGC 4 cut(s) 1684, 2836, 2975, 5128
PdmI GAANNNNTTC 3 cut(s) 1682, 2834, 3214
PfoI TCCNGGA 1 cut(s) 4051
PkrI GCNGC 1 cut(s) 366
PleI GAGTC 9 cut(s) 13, 239, 1522, 1594, 1738, 1882, 1968, 2616, 3801
PpsI GAGTC 9 cut(s) 13, 239, 1522, 1594, 1738, 1882, 1968, 2616, 3801
PscI ACATGT 2 cut(s) 3004, 3071
PshAI GACNNNNGTC 2 cut(s) 387, 3007
PsiI TTATAA 1 cut(s) 2949
Psp1406I AACGTT 1 cut(s) 3373
Psp6I CCWGG 2 cut(s) 681, 4051
PspFI CCCAGC 2 cut(s) 2971, 3122
PspGI CCWGG 2 cut(s) 681, 4051
PspN4I GGNNCC 6 cut(s) 70, 113, 656, 2969, 3696, 3847
PspPI GGNCC 5 cut(s) 382, 685, 696, 3695, 3917
PsrI GAACNNNNNNTAC 2 cut(s) 4561, 4593
PstNI CAGNNNCTG 1 cut(s) 4280
PsuI RGATCY 8 cut(s) 457, 532, 2967, 3307, 3451, 3955, 4418, 4993
RsaI GTAC 1 cut(s) 4228
RsaNI GTAC 1 cut(s) 4227
RseI CAYNNNNRTG 3 cut(s) 468, 3588, 4363
SatI GCNGC 1 cut(s) 365
Sau96I GGNCC 5 cut(s) 382, 685, 696, 3695, 3917
SchI GAGTC 9 cut(s) 14, 240, 1522, 1594, 1738, 1882, 1969, 2617, 3802
ScrFI CCNGG 4 cut(s) 683, 3699, 4053, 5016
SfcI CTRYAG 2 cut(s) 775, 3816
Sfr274I CTCGAG 1 cut(s) 4978
SfuI TTCGAA 4 cut(s) 3718, 3902, 4004, 4159
SinI GGWCC 3 cut(s) 382, 685, 3917
SlaI CTCGAG 1 cut(s) 4978
SmiMI CAYNNNNRTG 3 cut(s) 468, 3588, 4363
SmlI CTYRAG 4 cut(s) 116, 538, 766, 4978
SmoI CTYRAG 4 cut(s) 116, 538, 766, 4978
SpeI ACTAGT 1 cut(s) 3032
SsiI CCGC 5 cut(s) 3330, 3474, 3485, 3879, 5089
SspI AATATT 5 cut(s) 326, 377, 879, 1781, 3553
StyD4I CCNGG 4 cut(s) 681, 3697, 4051, 5014
TaaI ACNGT 5 cut(s) 68, 215, 3491, 3501, 4226
TaiI ACGT 4 cut(s) 2647, 2935, 3376, 4933
TaqI TCGA 9 cut(s) 2958, 3068, 3718, 3902, 4004, 4101, 4159, 4437, 4979
TaqII GACCGA 1 cut(s) 2495
TatI WGTACW 1 cut(s) 4226
TscAI CASTG 5 cut(s) 242, 484, 1309, 1381, 4281
TseFI GTSAC 8 cut(s) 389, 1986, 2634, 3126, 3631, 3758, 4743, 4823
TseI GCWGC 1 cut(s) 364
Tsp45I GTSAC 8 cut(s) 389, 1986, 2634, 3126, 3631, 3758, 4743, 4823
TspDTI ATGAA 9 cut(s) 180, 260, 905, 2668, 2968, 3141, 3572, 4200, 4546
TspRI CASTG 5 cut(s) 242, 484, 1309, 1381, 4281
VneI GTGCAC 1 cut(s) 132
VpaK11BI GGWCC 3 cut(s) 382, 685, 3917
XbaI TCTAGA 4 cut(s) 529, 3458, 4090, 4766
XceI RCATGY 3 cut(s) 29, 3008, 3075
XcmI CCANNNNNNNNNTGG 1 cut(s) 3786
XhoI CTCGAG 1 cut(s) 4978
XmiI GTMKAC 1 cut(s) 4577
XmnI GAANNNNTTC 3 cut(s) 1682, 2834, 3214
ZraI GACGTC 1 cut(s) 4931
Zsp2I ATGCAT 1 cut(s) 5107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.