Rmu_sc0002088.1_g000010

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002088.1
OG0001064
Physical Location & Seq
Reverse (-)
61288 .. 62171
884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002088.1_g000010.1.cds

Sequence Viewer

Length: 501 bp
atgggttattcgatctgggaaatagcgaaggccttcatcgacgaccactttgagctcgccgttgacctttacacccatgccattgccgtcgaccccaacaaatttgagttctactccgaccgcgctcaggccaacatcaaatccaacaatctcaccgctatgggtaaccaagatttgaatttgaacattggagctgtggatttggaattgaaggcaaaaatgtcaaaattggggctaagaaggtttctgggtaagtcggtatatagagtttctgatatggaacttgtgagaattctccctactgcagaagatgaggtcaacgtagcttgttttcatccttctgtcggaggtggccttgtttatggaactaaggaagggaagttaaggatcctccaatatgatagttctcatgccgtgggtcatacaacttccaattttcttgatgaaaacatgctagaggtacaacaaggccccagtcattcatttgtttgtttctgttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

166

Amino Acids

18.49

Weight (kDa)

5.44

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 90
AccII CGCG 1 cut(s) 123
AciI CCGC 2 cut(s) 121, 156
AclWI GGATC 2 cut(s) 382, 395
AcsI RAATTY 3 cut(s) 101, 178, 291
AfaI GTAC 1 cut(s) 462
AfiI CCNNNNNNNGG 2 cut(s) 127, 344
AgsI TTSAA 3 cut(s) 178, 184, 211
AluBI AGCT 3 cut(s) 55, 194, 326
AluI AGCT 3 cut(s) 55, 194, 326
Alw21I GWGCWC 1 cut(s) 57
AlwI GGATC 2 cut(s) 382, 395
AoxI GGCC 4 cut(s) 30, 129, 352, 469
ApoI RAATTY 3 cut(s) 101, 178, 291
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
Asp700I GAANNNNTTC 1 cut(s) 32
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 1 cut(s) 470
AsuHPI GGTGA 1 cut(s) 145
BamHI GGATCC 1 cut(s) 387
BanII GRGCYC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 57
BceAI ACGGC 3 cut(s) 44, 71, 398
BfaI CTAG 1 cut(s) 455
BfmI CTRYAG 1 cut(s) 303
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 2 cut(s) 389, 472
BmrI ACTGGG 1 cut(s) 468
BmuI ACTGGG 1 cut(s) 468
BplI GAGNNNNNCTC 2 cut(s) 98, 130
Bpu10I CCTNAGC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 414
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 344
Bse1I ACTGG 1 cut(s) 474
Bse3DI GCAATG 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 414
BseGI GGATG 1 cut(s) 334
BseLI CCNNNNNNNGG 2 cut(s) 127, 344
BseMI GCAATG 1 cut(s) 81
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 1 cut(s) 474
Bsh1236I CGCG 1 cut(s) 123
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 4 cut(s) 32, 131, 354, 471
BsiEI CGRYCG 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 57
BslI CCNNNNNNNGG 2 cut(s) 127, 344
BsnI GGCC 4 cut(s) 32, 131, 354, 471
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 2 cut(s) 12, 387
BspACI CCGC 2 cut(s) 121, 156
BspANI GGCC 4 cut(s) 32, 131, 354, 471
BspCNI CTCAG 1 cut(s) 139
BspFNI CGCG 1 cut(s) 123
BspLI GGNNCC 2 cut(s) 389, 472
BspMAI CTGCAG 1 cut(s) 307
BspPI GGATC 2 cut(s) 382, 395
BsrDI GCAATG 1 cut(s) 81
BsrI ACTGG 1 cut(s) 474
BssECI CCNNGG 1 cut(s) 414
BssMI GATC 2 cut(s) 12, 387
BstC8I GCNNGC 1 cut(s) 57
BstDEI CTNAG 3 cut(s) 126, 236, 369
BstDSI CCRYGG 1 cut(s) 414
BstEII GGTNACC 1 cut(s) 164
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 1 cut(s) 123
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 2 cut(s) 15, 390
BstMBI GATC 2 cut(s) 12, 387
BstMCI CGRYCG 1 cut(s) 121
BstNSI RCATGY 1 cut(s) 454
BstPI GGTNACC 1 cut(s) 164
BstSFI CTRYAG 1 cut(s) 303
BstUI CGCG 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 387
BstYI RGATCY 1 cut(s) 387
BsuRI GGCC 4 cut(s) 32, 131, 354, 471
BtgI CCRYGG 1 cut(s) 414
BtsCI GGATG 1 cut(s) 334
Cac8I GCNNGC 1 cut(s) 57
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 470
Csp6I GTAC 1 cut(s) 461
CviAII CATG 3 cut(s) 77, 410, 451
CviJI RGCY 8 cut(s) 32, 55, 131, 194, 235, 326, 354, 471
CviKI_1 RGCY 8 cut(s) 32, 55, 131, 194, 235, 326, 354, 471
CviQI GTAC 1 cut(s) 461
DdeI CTNAG 3 cut(s) 126, 236, 369
DpnI GATC 2 cut(s) 14, 389
DpnII GATC 2 cut(s) 12, 387
Ecl136II GAGCTC 1 cut(s) 55
Eco147I AGGCCT 1 cut(s) 32
Eco24I GRGCYC 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 55
Eco91I GGTNACC 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 55
EcoO109I RGGNCCY 1 cut(s) 470
EcoO65I GGTNACC 1 cut(s) 164
EcoRI GAATTC 1 cut(s) 291
EcoT38I GRGCYC 1 cut(s) 57
FaeI CATG 3 cut(s) 80, 413, 454
FatI CATG 3 cut(s) 76, 409, 450
FblI GTMKAC 1 cut(s) 90
FokI GGATG 1 cut(s) 321
FriOI GRGCYC 1 cut(s) 57
FspBI CTAG 1 cut(s) 455
GlaI GCGC 1 cut(s) 124
HaeIII GGCC 4 cut(s) 32, 131, 354, 471
HhaI GCGC 1 cut(s) 125
Hin1II CATG 3 cut(s) 80, 413, 454
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HincII GTYRAC 3 cut(s) 64, 91, 319
HindII GTYRAC 3 cut(s) 64, 91, 319
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 3 cut(s) 64, 91, 319
Hpy188I TCNGA 3 cut(s) 118, 274, 347
Hpy188III TCNNGA 1 cut(s) 440
Hpy8I GTNNAC 3 cut(s) 64, 91, 319
Hpy99I CGWCG 2 cut(s) 44, 92
HpyAV CCTTC 6 cut(s) 22, 43, 205, 234, 348, 368
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 1 cut(s) 305
HpyF3I CTNAG 3 cut(s) 126, 236, 369
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 3 cut(s) 80, 413, 454
HspAI GCGC 1 cut(s) 123
Kzo9I GATC 2 cut(s) 12, 387
LmnI GCTCC 1 cut(s) 191
LpnPI CCDG 3 cut(s) 113, 233, 487
MaeI CTAG 1 cut(s) 455
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 164
MalI GATC 2 cut(s) 14, 389
MboI GATC 2 cut(s) 12, 387
MboII GAAGA 1 cut(s) 320
MflI RGATCY 1 cut(s) 387
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 6 cut(s) 101, 178, 206, 227, 291, 433
MmeI TCCRAC 3 cut(s) 141, 168, 325
MnlI CCTC 4 cut(s) 307, 341, 401, 451
MroXI GAANNNNTTC 1 cut(s) 32
MseI TTAA 2 cut(s) 383, 499
MslI CAYNNNNRTG 1 cut(s) 158
MvnI CGCG 1 cut(s) 123
NdeII GATC 2 cut(s) 12, 387
NlaIII CATG 3 cut(s) 80, 413, 454
NlaIV GGNNCC 2 cut(s) 389, 472
NspI RCATGY 1 cut(s) 454
PceI AGGCCT 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 32
Psp124BI GAGCTC 1 cut(s) 57
PspEI GGTNACC 1 cut(s) 164
PspN4I GGNNCC 2 cut(s) 389, 472
PspPI GGNCC 1 cut(s) 470
PstI CTGCAG 1 cut(s) 307
PsuI RGATCY 1 cut(s) 387
RsaI GTAC 1 cut(s) 462
RsaNI GTAC 1 cut(s) 461
RseI CAYNNNNRTG 1 cut(s) 158
SacI GAGCTC 1 cut(s) 57
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 2 cut(s) 383, 499
Sau3AI GATC 2 cut(s) 12, 387
Sau96I GGNCC 1 cut(s) 470
SduI GDGCHC 1 cut(s) 57
SetI ASST 9 cut(s) 57, 69, 196, 245, 318, 324, 328, 352, 462
SfcI CTRYAG 1 cut(s) 303
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 6 cut(s) 101, 178, 206, 227, 291, 433
SseBI AGGCCT 1 cut(s) 32
SsiI CCGC 2 cut(s) 121, 156
SspMI CTAG 1 cut(s) 455
SstI GAGCTC 1 cut(s) 57
StuI AGGCCT 1 cut(s) 32
TaiI ACGT 1 cut(s) 324
TaqI TCGA 3 cut(s) 11, 39, 90
TasI AATT 6 cut(s) 101, 178, 206, 227, 291, 433
Tru1I TTAA 2 cut(s) 383, 499
Tru9I TTAA 2 cut(s) 383, 499
TspDTI ATGAA 4 cut(s) 25, 323, 459, 471
XapI RAATTY 3 cut(s) 101, 178, 291
XceI RCATGY 1 cut(s) 454
XmiI GTMKAC 1 cut(s) 90
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 1 cut(s) 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.