Rmu_sc0001292.1_g000022

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001292.1
OG0001064
Physical Location & Seq
Forward (+)
117468 .. 118365
898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001292.1_g000022.1.cds

Sequence Viewer

Length: 495 bp
atgggttgttcgatctgggaaatagcgaaggccttcatcgacgaccactttgagctcgccgttgacctttacacccatgccatcgccgtcgaccccaacaactctgagttctactccgaccgcgctcaggccaacatcaaatccaacaatctcaccggtaacaaccccctctctctcttgcaatttctgacgtcggttcttcaagctgtagaaaacctaatgggtagacctgttgtgttcttcatatctttcggcagtggcgtgttgctttcggcggattcgaaattcagctatggtgcagaagatgaggtcaatgtagcttgttttcatctttctgtcagaggtggcattgtttatggaactaaggaagggaagttaaggatcctccaatatgatagttctcatgccgtgggtcatacaacttccaattttcttgatgaaaacatgctagaggtacaacaaggccccagtcgtccatttgtttgtttatgttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

164

Amino Acids

18.01

Weight (kDa)

4.92

Isoelectric Point (pI)

30.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 194
AccI GTMKAC 2 cut(s) 90, 226
AccII CGCG 1 cut(s) 123
AciI CCGC 2 cut(s) 121, 275
AclWI GGATC 2 cut(s) 376, 389
AcsI RAATTY 1 cut(s) 284
AcyI GRCGYC 1 cut(s) 191
AfaI GTAC 1 cut(s) 456
AfiI CCNNNNNNNGG 1 cut(s) 127
AgeI ACCGGT 1 cut(s) 155
AgsI TTSAA 1 cut(s) 203
AluBI AGCT 4 cut(s) 55, 206, 291, 320
AluI AGCT 4 cut(s) 55, 206, 291, 320
Alw21I GWGCWC 1 cut(s) 57
AlwI GGATC 2 cut(s) 376, 389
AoxI GGCC 3 cut(s) 30, 129, 463
ApoI RAATTY 1 cut(s) 284
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
AsiGI ACCGGT 1 cut(s) 155
Asp700I GAANNNNTTC 1 cut(s) 32
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 1 cut(s) 464
AsuHPI GGTGA 1 cut(s) 145
AsuII TTCGAA 1 cut(s) 281
BamHI GGATCC 1 cut(s) 381
BanII GRGCYC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 57
BccI CCATC 1 cut(s) 89
BceAI ACGGC 3 cut(s) 44, 71, 392
BfaI CTAG 1 cut(s) 449
BfmI CTRYAG 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 2 cut(s) 383, 466
BmrI ACTGGG 1 cut(s) 462
BmuI ACTGGG 1 cut(s) 462
BplI GAGNNNNNCTC 2 cut(s) 98, 130
Bpu10I CCTNAGC 1 cut(s) 126
Bpu14I TTCGAA 1 cut(s) 281
BsaHI GRCGYC 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 408
BsaWI WCCGGW 1 cut(s) 155
Bsc4I CCNNNNNNNGG 1 cut(s) 127
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 468
BseDI CCNNGG 1 cut(s) 408
BseLI CCNNNNNNNGG 1 cut(s) 127
BseMII CTCAG 2 cut(s) 96, 140
BseNI ACTGG 1 cut(s) 468
BsgI GTGCAG 1 cut(s) 318
Bsh1236I CGCG 1 cut(s) 123
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 3 cut(s) 32, 131, 465
BshTI ACCGGT 1 cut(s) 155
BsiEI CGRYCG 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 57
BsiSI CCGG 1 cut(s) 156
BslI CCNNNNNNNGG 1 cut(s) 127
BsnI GGCC 3 cut(s) 32, 131, 465
Bsp119I TTCGAA 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 2 cut(s) 12, 381
BspACI CCGC 2 cut(s) 121, 275
BspANI GGCC 3 cut(s) 32, 131, 465
BspCNI CTCAG 2 cut(s) 97, 139
BspFNI CGCG 1 cut(s) 123
BspLI GGNNCC 2 cut(s) 383, 466
BspPI GGATC 2 cut(s) 376, 389
BspT104I TTCGAA 1 cut(s) 281
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 1 cut(s) 468
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 1 cut(s) 408
BssMI GATC 2 cut(s) 12, 381
BssNI GRCGYC 1 cut(s) 191
BstACI GRCGYC 1 cut(s) 191
BstBI TTCGAA 1 cut(s) 281
BstC8I GCNNGC 1 cut(s) 57
BstDEI CTNAG 3 cut(s) 105, 126, 363
BstDSI CCRYGG 1 cut(s) 408
BstFNI CGCG 1 cut(s) 123
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 2 cut(s) 15, 384
BstMBI GATC 2 cut(s) 12, 381
BstMCI CGRYCG 1 cut(s) 121
BstNSI RCATGY 1 cut(s) 448
BstSFI CTRYAG 1 cut(s) 207
BstUI CGCG 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 381
BstYI RGATCY 1 cut(s) 381
BsuRI GGCC 3 cut(s) 32, 131, 465
BtgI CCRYGG 1 cut(s) 408
BtgZI GCGATG 1 cut(s) 67
BtsI GCAGTG 1 cut(s) 262
BtsIMutI CAGTG 1 cut(s) 262
Cac8I GCNNGC 1 cut(s) 57
CfoI GCGC 1 cut(s) 125
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 464
Csp6I GTAC 1 cut(s) 455
CspAI ACCGGT 1 cut(s) 155
CviAII CATG 3 cut(s) 77, 404, 445
CviJI RGCY 7 cut(s) 32, 55, 131, 206, 291, 320, 465
CviKI_1 RGCY 7 cut(s) 32, 55, 131, 206, 291, 320, 465
CviQI GTAC 1 cut(s) 455
DdeI CTNAG 3 cut(s) 105, 126, 363
DpnI GATC 2 cut(s) 14, 383
DpnII GATC 2 cut(s) 12, 381
EciI GGCGGA 1 cut(s) 290
Ecl136II GAGCTC 1 cut(s) 55
Eco147I AGGCCT 1 cut(s) 32
Eco24I GRGCYC 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 55
EcoICRI GAGCTC 1 cut(s) 55
EcoO109I RGGNCCY 1 cut(s) 464
EcoT38I GRGCYC 1 cut(s) 57
FaeI CATG 3 cut(s) 80, 407, 448
FaiI YATR 9 cut(s) 78, 245, 294, 357, 393, 405, 417, 446, 490
FatI CATG 3 cut(s) 76, 403, 444
FblI GTMKAC 2 cut(s) 90, 226
FriOI GRGCYC 1 cut(s) 57
FspBI CTAG 1 cut(s) 449
GlaI GCGC 1 cut(s) 124
HaeIII GGCC 3 cut(s) 32, 131, 465
HapII CCGG 1 cut(s) 156
HhaI GCGC 1 cut(s) 125
Hin1I GRCGYC 1 cut(s) 191
Hin1II CATG 3 cut(s) 80, 407, 448
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HincII GTYRAC 2 cut(s) 64, 91
HindII GTYRAC 2 cut(s) 64, 91
HinfI GANTC 1 cut(s) 278
HpaII CCGG 1 cut(s) 156
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 3 cut(s) 64, 91, 227
Hpy188I TCNGA 4 cut(s) 106, 118, 189, 341
Hpy188III TCNNGA 1 cut(s) 434
Hpy8I GTNNAC 3 cut(s) 64, 91, 227
Hpy99I CGWCG 3 cut(s) 44, 92, 196
HpyAV CCTTC 3 cut(s) 22, 43, 362
HpyCH4IV ACGT 1 cut(s) 191
HpyCH4V TGCA 2 cut(s) 181, 299
HpyF3I CTNAG 3 cut(s) 105, 126, 363
HpySE526I ACGT 1 cut(s) 191
Hsp92I GRCGYC 1 cut(s) 191
Hsp92II CATG 3 cut(s) 80, 407, 448
HspAI GCGC 1 cut(s) 123
Kzo9I GATC 2 cut(s) 12, 381
LpnPI CCDG 4 cut(s) 113, 169, 243, 481
MaeI CTAG 1 cut(s) 449
MaeII ACGT 1 cut(s) 191
MaeIII GTNAC 1 cut(s) 158
MalI GATC 2 cut(s) 14, 383
MboI GATC 2 cut(s) 12, 381
MboII GAAGA 3 cut(s) 191, 232, 314
MflI RGATCY 1 cut(s) 381
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 3 cut(s) 182, 284, 427
MmeI TCCRAC 2 cut(s) 141, 168
MnlI CCTC 5 cut(s) 179, 301, 335, 395, 445
MroXI GAANNNNTTC 1 cut(s) 32
MseI TTAA 2 cut(s) 377, 493
MspI CCGG 1 cut(s) 156
MvnI CGCG 1 cut(s) 123
NdeII GATC 2 cut(s) 12, 381
NlaIII CATG 3 cut(s) 80, 407, 448
NlaIV GGNNCC 2 cut(s) 383, 466
NspI RCATGY 1 cut(s) 448
NspV TTCGAA 1 cut(s) 281
PceI AGGCCT 1 cut(s) 32
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 278
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 1 cut(s) 278
PinAI ACCGGT 1 cut(s) 155
Psp124BI GAGCTC 1 cut(s) 57
PspN4I GGNNCC 2 cut(s) 383, 466
PspPI GGNCC 1 cut(s) 464
PsuI RGATCY 1 cut(s) 381
RsaI GTAC 1 cut(s) 456
RsaNI GTAC 1 cut(s) 455
SacI GAGCTC 1 cut(s) 57
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 2 cut(s) 377, 493
Sau3AI GATC 2 cut(s) 12, 381
Sau96I GGNCC 1 cut(s) 464
SduI GDGCHC 1 cut(s) 57
SfcI CTRYAG 1 cut(s) 207
SfuI TTCGAA 1 cut(s) 281
Sse9I AATT 3 cut(s) 182, 284, 427
SseBI AGGCCT 1 cut(s) 32
SsiI CCGC 2 cut(s) 121, 275
SspMI CTAG 1 cut(s) 449
SstI GAGCTC 1 cut(s) 57
StuI AGGCCT 1 cut(s) 32
TaiI ACGT 1 cut(s) 194
TaqI TCGA 4 cut(s) 11, 39, 90, 281
TasI AATT 3 cut(s) 182, 284, 427
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 2 cut(s) 377, 493
Tru9I TTAA 2 cut(s) 377, 493
TscAI CASTG 1 cut(s) 262
TspDTI ATGAA 4 cut(s) 25, 232, 317, 453
TspRI CASTG 1 cut(s) 262
XapI RAATTY 1 cut(s) 284
XceI RCATGY 1 cut(s) 448
XmiI GTMKAC 2 cut(s) 90, 226
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 1 cut(s) 449
ZraI GACGTC 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.