Rroxscaffold_4G00304060

helicase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000621
Physical Location & Seq
Forward (+)
24695765 .. 24697679
1915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00304060.1

Sequence Viewer

Length: 822 bp
ATGGGCGGCGGGCCCAGACCTTCCCGGCGGTGTATCCAAGTGGTAGTGGAAGCGAATGCAGGCCAAGAAAGCCAAGCATCTCCTCAAGGCCTGCCTCGCCGTGAGCGCCAAATCTACGAGATACGAAGGCGGGCCGAGCTCAAAGCCGCGGTGTCGAAGCTCGACGGAGACCGTCGAGAACCTCCCAATTTGTTCTCCGTCAACACCGATGAGCAGGTTAAGGTCTTGGCCGATCGGTTCCGAGGGCCGGGAGGGTTTGACTTGTGGATCGAGAAGAATGGGCCGCAGCTGTTTCGAACCATGGAGGACTTGTTGTCGGCGAAGTTTTTTCCCAAGGGGGTTGTGCATAGTGAGGCCGTATCGGAGGATTGGGGCCGAGGAATTGGGGGAGGAGAGGGAGCGAAGAGGAGGAGTGTAATACCCGCAATTTTATTTTCGTCTCGTAAAATTGTGCAAATTGATTTAAACAAAGTCATCCTCTTTCTCTCCACTTCGGCCAGCTCCCAGCCTGCACTATCTCTCGCACTCGCTCCGGACCTTCCTCCTTTTCCCCGATTCCGATTTCCGATTTCAATTCCGATCGACGCCTCCCCTCTGTCACAAGAGCTCGATCCAATTAAGAAGGCAGAAGTTGAACTTTCGAAACCGGTGAAATCTGTTGTTTTAGTCACCGAGGAGGAGGATATTGGCCCCGCCGCATGGAAGAGTATGATGAAGAAAATCCAAGAAGTACCACTAAATACGACCACCATTTCCACCTTGCTTGCATTCTTGAATGGATGGAAAGAAGTGACTCATGTCCGTGTGCGATCAGGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

273

Amino Acids

30.14

Weight (kDa)

9.67

Isoelectric Point (pI)

57.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000621)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g32560 FvH4_3g35880 FvH4_4g19501 FvH4_4g19530 FvH4_4g19531 FvH4_4g19540 FvH4_4g19540
prunus_persica Prupe.1G121600_v2.0.a1 Prupe.1G121700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0416301 RchiOBHm_Chr4g0424661
rosa_laevigata RLG00000007388 RLG00000007432 RLG00000007434 RLG00000007435 RLG00000007439 RLG00000007441 RLG00000007442 RLG00000007458 RLG00000007465 RLG00000007997 RLG00000008015 RLG00000008016 RLG00000008017 RLG00000035315
rosa_multiflora Rmu_co8150926.1_g000001 Rmu_co8225682.1_g000001 Rmu_co8296077.1_g000001 Rmu_sc0004841.1_g000022 Rmu_sc0006553.1_g000008 Rmu_sc0008889.1_g000002
rosa_roxburghii Rroxscaffold_1G00030220 Rroxscaffold_2G00114820 Rroxscaffold_3G00219510 Rroxscaffold_3G00255600 Rroxscaffold_4G00304060 Rroxscaffold_4G00330360 Rroxscaffold_4G00330400 Rroxscaffold_5G00359920 Rroxscaffold_5G00359930 Rroxscaffold_5G00359960 Rroxscaffold_5G00366600 Rroxscaffold_5G00366630 Rroxscaffold_5G00366640 Rroxscaffold_5G00366710 Rroxscaffold_5G00366970
rosa_rugosa Rorug04G0138400 Rorug04G0138500 Rorug04G0138700 Rorug04G0140600 Rorug04G0199000 Rorug07G0061600
rosa_samantha Rh4AG255800 Rh4AG255900 Rh4AG256000 Rh4AG256200 Rh4BG261200 Rh4BG261300 Rh4BG261600 Rh4CG212100 Rh4CG212800 Rh4CG212900 Rh4CG272300 Rh4CG272500 Rh4CG272600 Rh4CG272800 Rh4CG275800 Rh4DG255800 Rh4DG255900 Rh4DG256200 Rh4DG257200 Rh4DG257300 Rh7BG190600 Rh7CG198100
rosa_wichuraiana Rw1G001980 Rw4G022090 Rw4G024000 Rw4G024010 Rw7G016430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 205
AccII CGCG 1 cut(s) 149
AccIII TCCGGA 1 cut(s) 532
AclWI GGATC 2 cut(s) 275, 605
AcoI YGGCCR 2 cut(s) 228, 495
AcyI GRCGYC 1 cut(s) 585
AfaI GTAC 1 cut(s) 732
AfiI CCNNNNNNNGG 2 cut(s) 247, 699
AgeI ACCGGT 1 cut(s) 646
AgsI TTSAA 3 cut(s) 573, 635, 775
AhdI GACNNNNNGTC 1 cut(s) 313
AluBI AGCT 5 cut(s) 139, 160, 289, 501, 607
AluI AGCT 5 cut(s) 139, 160, 289, 501, 607
Alw21I GWGCWC 2 cut(s) 141, 609
Alw26I GTCTC 2 cut(s) 162, 444
AlwI GGATC 2 cut(s) 275, 605
Aor13HI TCCGGA 1 cut(s) 532
ApaI GGGCCC 1 cut(s) 15
ApeKI GCWGC 1 cut(s) 286
AsiGI ACCGGT 1 cut(s) 646
AspLEI GCGC 1 cut(s) 108
AspS9I GGNCC 8 cut(s) 11, 12, 132, 245, 281, 373, 535, 689
AsuC2I CCSGG 2 cut(s) 25, 249
AsuHPI GGTGA 2 cut(s) 661, 661
AsuII TTCGAA 2 cut(s) 295, 641
AvaII GGWCC 1 cut(s) 535
BaeGI GKGCMC 1 cut(s) 15
BanII GRGCYC 3 cut(s) 15, 141, 609
Bbv12I GWGCWC 2 cut(s) 141, 609
BbvI GCAGC 1 cut(s) 298
BccI CCATC 1 cut(s) 774
BceAI ACGGC 2 cut(s) 84, 341
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BciVI GTATCC 1 cut(s) 44
BcnI CCSGG 2 cut(s) 25, 249
BcoDI GTCTC 2 cut(s) 162, 444
BfoI RGCGCY 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 205
BfuI GTATCC 1 cut(s) 44
BisI GCNGC 5 cut(s) 7, 147, 284, 287, 696
BlsI GCNGC 5 cut(s) 8, 148, 285, 288, 697
Bme1390I CCNGG 2 cut(s) 25, 249
Bme18I GGWCC 1 cut(s) 535
BmeRI GACNNNNNGTC 1 cut(s) 313
BmgT120I GGNCC 8 cut(s) 11, 12, 132, 245, 281, 373, 535, 689
BmiI GGNNCC 4 cut(s) 13, 239, 374, 691
BmrFI CCNGG 2 cut(s) 25, 249
BmsI GCATC 1 cut(s) 86
BoxI GACNNNNGTC 1 cut(s) 797
Bpu14I TTCGAA 2 cut(s) 295, 641
BpuEI CTTGAG 1 cut(s) 69
BpuMI CCSGG 2 cut(s) 25, 249
BsaHI GRCGYC 1 cut(s) 585
BsaI GGTCTC 1 cut(s) 162
BsaJI CCNNGG 6 cut(s) 147, 241, 300, 333, 376, 672
BsaWI WCCGGW 2 cut(s) 532, 646
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 699
Bse118I RCCGGY 1 cut(s) 646
BseAI TCCGGA 1 cut(s) 532
BseDI CCNNGG 6 cut(s) 147, 241, 300, 333, 376, 672
BseGI GGATG 2 cut(s) 474, 785
BseLI CCNNNNNNNGG 2 cut(s) 247, 699
BseRI GAGGAG 6 cut(s) 72, 405, 421, 424, 689, 692
BseSI GKGCMC 1 cut(s) 15
BseXI GCAGC 1 cut(s) 298
BseYI CCCAGC 1 cut(s) 504
BsgI GTGCAG 1 cut(s) 495
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 2 cut(s) 235, 582
BshTI ACCGGT 1 cut(s) 646
BsiEI CGRYCG 2 cut(s) 235, 582
BsiHKAI GWGCWC 2 cut(s) 141, 609
BsiSI CCGG 4 cut(s) 25, 248, 533, 647
BslI CCNNNNNNNGG 2 cut(s) 247, 699
BsmAI GTCTC 2 cut(s) 162, 444
BsmBI CGTCTC 1 cut(s) 444
BsmI GAATGC 2 cut(s) 61, 767
Bso31I GGTCTC 1 cut(s) 162
Bsp119I TTCGAA 2 cut(s) 295, 641
Bsp120I GGGCCC 1 cut(s) 11
Bsp1286I GDGCHC 3 cut(s) 15, 141, 609
Bsp13I TCCGGA 1 cut(s) 532
Bsp143I GATC 5 cut(s) 232, 267, 579, 610, 809
Bsp19I CCATGG 1 cut(s) 300
BspEI TCCGGA 1 cut(s) 532
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 4 cut(s) 13, 239, 374, 691
BspMI ACCTGC 1 cut(s) 205
BspPI GGATC 2 cut(s) 275, 605
BspT104I TTCGAA 2 cut(s) 295, 641
BspTNI GGTCTC 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 646
BssAI RCCGGY 1 cut(s) 646
BssECI CCNNGG 6 cut(s) 147, 241, 300, 333, 376, 672
BssMI GATC 5 cut(s) 232, 267, 579, 610, 809
BssNI GRCGYC 1 cut(s) 585
BssT1I CCWWGG 2 cut(s) 300, 333
Bst4CI ACNGT 1 cut(s) 173
Bst6I CTCTTC 2 cut(s) 398, 698
BstACI GRCGYC 1 cut(s) 585
BstBI TTCGAA 2 cut(s) 295, 641
BstC8I GCNNGC 7 cut(s) 11, 61, 92, 132, 499, 510, 765
BstDSI CCRYGG 2 cut(s) 147, 300
BstF5I GGATG 2 cut(s) 474, 785
BstFNI CGCG 1 cut(s) 149
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 1 cut(s) 108
BstKTI GATC 5 cut(s) 235, 270, 582, 613, 812
BstMAI GTCTC 2 cut(s) 162, 444
BstMBI GATC 5 cut(s) 232, 267, 579, 610, 809
BstMCI CGRYCG 2 cut(s) 235, 582
BstMWI GCNNNNNNNGC 4 cut(s) 69, 96, 105, 136
BstPAI GACNNNNGTC 1 cut(s) 797
BstSCI CCNGG 2 cut(s) 23, 247
BstSLI GKGCMC 1 cut(s) 15
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 1 cut(s) 298
BsuI GTATCC 1 cut(s) 44
BtgI CCRYGG 2 cut(s) 147, 300
BtsCI GGATG 2 cut(s) 474, 785
BveI ACCTGC 1 cut(s) 205
Cac8I GCNNGC 7 cut(s) 11, 61, 92, 132, 499, 510, 765
CfoI GCGC 1 cut(s) 108
Cfr10I RCCGGY 1 cut(s) 646
Cfr13I GGNCC 8 cut(s) 11, 12, 132, 245, 281, 373, 535, 689
Cfr42I CCGCGG 1 cut(s) 150
CseI GACGC 1 cut(s) 593
Csp6I GTAC 1 cut(s) 731
CspAI ACCGGT 1 cut(s) 646
CspCI CAANNNNNGTGG 2 cut(s) 745, 780
CviAII CATG 3 cut(s) 301, 699, 797
CviQI GTAC 1 cut(s) 731
DpnI GATC 5 cut(s) 234, 269, 581, 612, 811
DpnII GATC 5 cut(s) 232, 267, 579, 610, 809
DraI TTTAAA 1 cut(s) 465
DriI GACNNNNNGTC 1 cut(s) 313
EaeI YGGCCR 2 cut(s) 228, 495
Eam1104I CTCTTC 2 cut(s) 398, 698
Eam1105I GACNNNNNGTC 1 cut(s) 313
EarI CTCTTC 2 cut(s) 398, 698
Ecl136II GAGCTC 2 cut(s) 139, 607
Eco130I CCWWGG 2 cut(s) 300, 333
Eco147I AGGCCT 1 cut(s) 90
Eco24I GRGCYC 3 cut(s) 15, 141, 609
Eco31I GGTCTC 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 535
Eco53kI GAGCTC 2 cut(s) 139, 607
EcoICRI GAGCTC 2 cut(s) 139, 607
EcoT14I CCWWGG 2 cut(s) 300, 333
EcoT38I GRGCYC 3 cut(s) 15, 141, 609
ErhI CCWWGG 2 cut(s) 300, 333
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 3 cut(s) 304, 702, 800
FaiI YATR 5 cut(s) 302, 348, 700, 710, 798
FalI AAGNNNNNCTT 2 cut(s) 621, 653
FatI CATG 3 cut(s) 300, 698, 796
FauI CCCGC 4 cut(s) 2, 123, 430, 700
Fnu4HI GCNGC 5 cut(s) 7, 147, 284, 287, 696
FokI GGATG 2 cut(s) 461, 792
FriOI GRGCYC 3 cut(s) 15, 141, 609
Fsp4HI GCNGC 5 cut(s) 7, 147, 284, 287, 696
GlaI GCGC 1 cut(s) 107
GluI GCNGC 5 cut(s) 7, 147, 284, 287, 696
GsaI CCCAGC 1 cut(s) 508
HaeII RGCGCY 1 cut(s) 109
HapII CCGG 4 cut(s) 25, 248, 533, 647
HgaI GACGC 1 cut(s) 593
HhaI GCGC 1 cut(s) 108
Hin1I GRCGYC 1 cut(s) 585
Hin1II CATG 3 cut(s) 304, 702, 800
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 2 cut(s) 555, 793
HpaII CCGG 4 cut(s) 25, 248, 533, 647
HphI GGTGA 2 cut(s) 661, 661
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 5 cut(s) 242, 364, 560, 567, 579
Hpy188III TCNNGA 4 cut(s) 176, 271, 533, 772
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 3 cut(s) 167, 177, 587
HpyAV CCTTC 4 cut(s) 30, 120, 548, 616
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4V TGCA 5 cut(s) 59, 346, 454, 512, 767
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 96, 105, 136
Hsp92I GRCGYC 1 cut(s) 585
Hsp92II CATG 3 cut(s) 304, 702, 800
HspAI GCGC 1 cut(s) 106
Kpn2I TCCGGA 1 cut(s) 532
KspI CCGCGG 1 cut(s) 150
Kzo9I GATC 5 cut(s) 232, 267, 579, 610, 809
LmnI GCTCC 3 cut(s) 398, 506, 535
Lsp1109I GCAGC 1 cut(s) 298
LweI GCATC 1 cut(s) 86
MaeIII GTNAC 3 cut(s) 597, 667, 790
MalI GATC 5 cut(s) 234, 269, 581, 612, 811
MboI GATC 5 cut(s) 232, 267, 579, 610, 809
MboII GAAGA 4 cut(s) 286, 415, 715, 727
MhlI GDGCHC 3 cut(s) 15, 141, 609
MluCI AATT 7 cut(s) 187, 381, 426, 447, 456, 573, 615
MlyI GAGTC 1 cut(s) 787
MroI TCCGGA 1 cut(s) 532
MseI TTAA 3 cut(s) 219, 464, 618
MslI CAYNNNNRTG 1 cut(s) 801
MspA1I CMGCKG 2 cut(s) 149, 289
MspI CCGG 4 cut(s) 25, 248, 533, 647
MspR9I CCNGG 2 cut(s) 25, 249
Mva1269I GAATGC 2 cut(s) 61, 767
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 4 cut(s) 69, 96, 105, 136
NciI CCSGG 2 cut(s) 25, 249
NcoI CCATGG 1 cut(s) 300
NdeII GATC 5 cut(s) 232, 267, 579, 610, 809
NlaIII CATG 3 cut(s) 304, 702, 800
NlaIV GGNNCC 4 cut(s) 13, 239, 374, 691
NmeAIII GCCGAG 2 cut(s) 160, 401
NmuCI GTSAC 3 cut(s) 597, 667, 790
NspV TTCGAA 2 cut(s) 295, 641
PceI AGGCCT 1 cut(s) 90
PctI GAATGC 2 cut(s) 61, 767
PfeI GAWTC 1 cut(s) 555
PinAI ACCGGT 1 cut(s) 646
PkrI GCNGC 5 cut(s) 8, 148, 285, 288, 697
Ple19I CGATCG 2 cut(s) 235, 582
PleI GAGTC 1 cut(s) 787
PpsI GAGTC 1 cut(s) 787
PshAI GACNNNNGTC 1 cut(s) 797
Psp124BI GAGCTC 2 cut(s) 141, 609
PspFI CCCAGC 1 cut(s) 504
PspN4I GGNNCC 4 cut(s) 13, 239, 374, 691
PspOMI GGGCCC 1 cut(s) 11
PspPI GGNCC 8 cut(s) 11, 12, 132, 245, 281, 373, 535, 689
PvuI CGATCG 2 cut(s) 235, 582
PvuII CAGCTG 1 cut(s) 289
RsaI GTAC 1 cut(s) 732
RsaNI GTAC 1 cut(s) 731
RseI CAYNNNNRTG 1 cut(s) 801
SacI GAGCTC 2 cut(s) 141, 609
SacII CCGCGG 1 cut(s) 150
SaqAI TTAA 3 cut(s) 219, 464, 618
SatI GCNGC 5 cut(s) 7, 147, 284, 287, 696
Sau3AI GATC 5 cut(s) 232, 267, 579, 610, 809
Sau96I GGNCC 8 cut(s) 11, 12, 132, 245, 281, 373, 535, 689
SchI GAGTC 1 cut(s) 787
ScrFI CCNGG 2 cut(s) 25, 249
SduI GDGCHC 3 cut(s) 15, 141, 609
SfaNI GCATC 1 cut(s) 86
Sfr303I CCGCGG 1 cut(s) 150
SfuI TTCGAA 2 cut(s) 295, 641
SgrBI CCGCGG 1 cut(s) 150
SinI GGWCC 1 cut(s) 535
SmiMI CAYNNNNRTG 1 cut(s) 801
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
Sse9I AATT 7 cut(s) 187, 381, 426, 447, 456, 573, 615
SseBI AGGCCT 1 cut(s) 90
SstI GAGCTC 2 cut(s) 141, 609
StuI AGGCCT 1 cut(s) 90
StyD4I CCNGG 2 cut(s) 23, 247
StyI CCWWGG 2 cut(s) 300, 333
TaaI ACNGT 1 cut(s) 173
TaqI TCGA 8 cut(s) 155, 162, 175, 270, 295, 582, 609, 641
TasI AATT 7 cut(s) 187, 381, 426, 447, 456, 573, 615
TauI GCSGC 4 cut(s) 9, 149, 286, 698
TfiI GAWTC 1 cut(s) 555
Tru1I TTAA 3 cut(s) 219, 464, 618
Tru9I TTAA 3 cut(s) 219, 464, 618
TseFI GTSAC 3 cut(s) 597, 667, 790
TseI GCWGC 1 cut(s) 286
Tsp45I GTSAC 3 cut(s) 597, 667, 790
TspDTI ATGAA 1 cut(s) 728
TspGWI ACGGA 3 cut(s) 180, 187, 791
VpaK11BI GGWCC 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.