Rh4DG256200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000621
Physical Location & Seq
Reverse (-)
47196472 .. 47197648
1177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG256200.1

Sequence Viewer

Length: 360 bp
ATGAATCTCACCCATCCAATATACGAGTCTGGTATGCCGATGCAGCATAGTCAAGATCCAATGACGCAGGCACCATTTCCTTATCCAGGCTATCCCCCTAATCCCTATTTGGTTTATCCTCATTCTCCAGCTCCTGCTCATCCTAGTACTCCATATGGATCATCATCATCATACTATCACGATCCATATTATGGAATTCCAGCTCCTGTACAGCTCGCTCCTGCTCCACCTAATAAAACCAAGCCCAAGAGAAGCAAAACTGTAGGAAAAAAAACATCTTATACCGTCAGCGGTAATCGAGTTAGTCAAAACTCAGGCGACAATCACCGAATTTTCAACATTGGCGACCATACTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

119

Amino Acids

13.11

Weight (kDa)

9.33

Isoelectric Point (pI)

58.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000621)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g32560 FvH4_3g35880 FvH4_4g19501 FvH4_4g19530 FvH4_4g19531 FvH4_4g19540 FvH4_4g19540
prunus_persica Prupe.1G121600_v2.0.a1 Prupe.1G121700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0416301 RchiOBHm_Chr4g0424661
rosa_laevigata RLG00000007388 RLG00000007432 RLG00000007434 RLG00000007435 RLG00000007439 RLG00000007441 RLG00000007442 RLG00000007458 RLG00000007465 RLG00000007997 RLG00000008015 RLG00000008016 RLG00000008017 RLG00000035315
rosa_multiflora Rmu_co8150926.1_g000001 Rmu_co8225682.1_g000001 Rmu_co8296077.1_g000001 Rmu_sc0004841.1_g000022 Rmu_sc0006553.1_g000008 Rmu_sc0008889.1_g000002
rosa_roxburghii Rroxscaffold_1G00030220 Rroxscaffold_2G00114820 Rroxscaffold_3G00219510 Rroxscaffold_3G00255600 Rroxscaffold_4G00304060 Rroxscaffold_4G00330360 Rroxscaffold_4G00330400 Rroxscaffold_5G00359920 Rroxscaffold_5G00359930 Rroxscaffold_5G00359960 Rroxscaffold_5G00366600 Rroxscaffold_5G00366630 Rroxscaffold_5G00366640 Rroxscaffold_5G00366710 Rroxscaffold_5G00366970
rosa_rugosa Rorug04G0138400 Rorug04G0138500 Rorug04G0138700 Rorug04G0140600 Rorug04G0199000 Rorug07G0061600
rosa_samantha Rh4AG255800 Rh4AG255900 Rh4AG256000 Rh4AG256200 Rh4BG261200 Rh4BG261300 Rh4BG261600 Rh4CG212100 Rh4CG212800 Rh4CG212900 Rh4CG272300 Rh4CG272500 Rh4CG272600 Rh4CG272800 Rh4CG275800 Rh4DG255800 Rh4DG255900 Rh4DG256200 Rh4DG257200 Rh4DG257300 Rh7BG190600 Rh7CG198100
rosa_wichuraiana Rw1G001980 Rw4G022090 Rw4G024000 Rw4G024010 Rw7G016430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 70
AccB7I CCANNNNNTGG 1 cut(s) 191
AciI CCGC 1 cut(s) 291
AclWI GGATC 3 cut(s) 50, 166, 176
AcsI RAATTY 2 cut(s) 195, 330
AfaI GTAC 2 cut(s) 148, 210
AfiI CCNNNNNNNGG 2 cut(s) 86, 191
AgsI TTSAA 1 cut(s) 337
AjnI CCWGG 1 cut(s) 85
AluBI AGCT 3 cut(s) 131, 203, 214
AluI AGCT 3 cut(s) 131, 203, 214
AlwI GGATC 3 cut(s) 50, 166, 176
AlwNI CAGNNNCTG 2 cut(s) 134, 206
ApeKI GCWGC 1 cut(s) 43
ApoI RAATTY 2 cut(s) 195, 330
AsuHPI GGTGA 1 cut(s) 317
BanI GGYRCC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 55
BccI CCATC 1 cut(s) 21
BciT130I CCWGG 1 cut(s) 87
BfaI CTAG 1 cut(s) 144
BfmI CTRYAG 1 cut(s) 261
BisI GCNGC 1 cut(s) 44
BlsI GCNGC 1 cut(s) 45
BmcAI AGTACT 1 cut(s) 148
Bme1390I CCNGG 1 cut(s) 87
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 1 cut(s) 30
BpmI CTGGAG 1 cut(s) 111
BsaBI GATNNNNATC 1 cut(s) 163
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 191
Bse8I GATNNNNATC 1 cut(s) 163
BseBI CCWGG 1 cut(s) 87
BseGI GGATG 2 cut(s) 13, 139
BseJI GATNNNNATC 1 cut(s) 163
BseLI CCNNNNNNNGG 2 cut(s) 86, 191
BseMII CTCAG 1 cut(s) 327
BseXI GCAGC 1 cut(s) 55
BshNI GGYRCC 1 cut(s) 70
BslI CCNNNNNNNGG 2 cut(s) 86, 191
Bsp1407I TGTACA 1 cut(s) 208
Bsp143I GATC 3 cut(s) 55, 158, 181
BspACI CCGC 1 cut(s) 291
BspCNI CTCAG 1 cut(s) 326
BspLI GGNNCC 1 cut(s) 72
BspPI GGATC 3 cut(s) 50, 166, 176
BspT107I GGYRCC 1 cut(s) 70
BsrGI TGTACA 1 cut(s) 208
BssMI GATC 3 cut(s) 55, 158, 181
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 2 cut(s) 262, 286
BstAUI TGTACA 1 cut(s) 208
BstC8I GCNNGC 2 cut(s) 69, 216
BstDEI CTNAG 1 cut(s) 313
BstENI CCTNNNNNAGG 1 cut(s) 84
BstF5I GGATG 2 cut(s) 13, 139
BstKTI GATC 3 cut(s) 58, 161, 184
BstMBI GATC 3 cut(s) 55, 158, 181
BstMWI GCNNNNNNNGC 1 cut(s) 43
BstNI CCWGG 1 cut(s) 87
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 261
BstV1I GCAGC 1 cut(s) 55
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
BtsCI GGATG 2 cut(s) 13, 139
Cac8I GCNNGC 2 cut(s) 69, 216
CaiI CAGNNNCTG 2 cut(s) 134, 206
CseI GACGC 1 cut(s) 73
Csp6I GTAC 2 cut(s) 147, 209
CviJI RGCY 5 cut(s) 90, 131, 203, 214, 244
CviKI_1 RGCY 5 cut(s) 90, 131, 203, 214, 244
CviQI GTAC 2 cut(s) 147, 209
DdeI CTNAG 1 cut(s) 313
DpnI GATC 3 cut(s) 57, 160, 183
DpnII GATC 3 cut(s) 55, 158, 181
EcoNI CCTNNNNNAGG 1 cut(s) 84
EcoRI GAATTC 1 cut(s) 195
EcoRII CCWGG 1 cut(s) 85
FauNDI CATATG 1 cut(s) 154
Fnu4HI GCNGC 1 cut(s) 44
FokI GGATG 1 cut(s) 126
Fsp4HI GCNGC 1 cut(s) 44
FspBI CTAG 1 cut(s) 144
GluI GCNGC 1 cut(s) 44
GsuI CTGGAG 1 cut(s) 111
HgaI GACGC 1 cut(s) 73
HinfI GANTC 2 cut(s) 4, 26
HphI GGTGA 1 cut(s) 317
Hpy188III TCNNGA 2 cut(s) 53, 179
HpyCH4III ACNGT 2 cut(s) 262, 286
HpyCH4V TGCA 1 cut(s) 43
HpyF10VI GCNNNNNNNGC 1 cut(s) 43
HpyF3I CTNAG 1 cut(s) 313
Kzo9I GATC 3 cut(s) 55, 158, 181
LmnI GCTCC 4 cut(s) 136, 208, 223, 229
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 1 cut(s) 30
MaeI CTAG 1 cut(s) 144
MalI GATC 3 cut(s) 57, 160, 183
MboI GATC 3 cut(s) 55, 158, 181
MflI RGATCY 1 cut(s) 55
MluCI AATT 2 cut(s) 195, 330
MlyI GAGTC 1 cut(s) 35
MnlI CCTC 1 cut(s) 129
MseI TTAA 1 cut(s) 358
MspA1I CMGCKG 1 cut(s) 291
MspR9I CCNGG 1 cut(s) 87
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 1 cut(s) 43
NdeI CATATG 1 cut(s) 154
NdeII GATC 3 cut(s) 55, 158, 181
NlaIV GGNNCC 1 cut(s) 72
PfeI GAWTC 1 cut(s) 4
PflMI CCANNNNNTGG 1 cut(s) 191
PkrI GCNGC 1 cut(s) 45
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
Psp6I CCWGG 1 cut(s) 85
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 1 cut(s) 72
PstNI CAGNNNCTG 2 cut(s) 134, 206
PsuI RGATCY 1 cut(s) 55
RsaI GTAC 2 cut(s) 148, 210
RsaNI GTAC 2 cut(s) 147, 209
SaqAI TTAA 1 cut(s) 358
SatI GCNGC 1 cut(s) 44
Sau3AI GATC 3 cut(s) 55, 158, 181
ScaI AGTACT 1 cut(s) 148
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 1 cut(s) 87
SetI ASST 4 cut(s) 133, 205, 216, 232
SfaNI GCATC 1 cut(s) 30
SfcI CTRYAG 1 cut(s) 261
Sse9I AATT 2 cut(s) 195, 330
SsiI CCGC 1 cut(s) 291
SspMI CTAG 1 cut(s) 144
StyD4I CCNGG 1 cut(s) 85
TaaI ACNGT 2 cut(s) 262, 286
TaqI TCGA 1 cut(s) 298
TasI AATT 2 cut(s) 195, 330
TatI WGTACW 2 cut(s) 146, 208
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 1 cut(s) 358
Tru9I TTAA 1 cut(s) 358
TseI GCWGC 1 cut(s) 43
TspDTI ATGAA 1 cut(s) 17
Van91I CCANNNNNTGG 1 cut(s) 191
XagI CCTNNNNNAGG 1 cut(s) 84
XapI RAATTY 2 cut(s) 195, 330
XspI CTAG 1 cut(s) 144
ZrmI AGTACT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.