Rh4AG123500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001347
Physical Location & Seq
Forward (+)
26633423 .. 26634691
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG123500.1

Sequence Viewer

Length: 720 bp
ATGGTTTGTTTAAGGCCAGATTCTAGAGTTAAAAAGGGTCCGCCTCGTAATCCTGCAGCCTCTGCTAATCACTCTTTAAATGCTCGTGGTCGCTTTGCTCCTCGTTTTAATTTTCGCCGCCCTAGCGTCACTTTTGTTGATGCTACTCGCAATTTGCATGACACTGTGGAAGTTGACAATTCTCCTGTTCACCATCATGCACATAAGCCACCCTTGGTTCCTGCTAGTTATGTTTCCCAAACTTTGGTGGTTGCTGCTAAGGAACCTAAGCCTCTTGGTATTATCCTCCCTAATGGTGAGTCTGATCTCAACTTTAATACTGATGACCTTGTTGCTGATGAGGAACTAGATCAGGATTTGGAGGCAGATGTTGAAGAAGATGTTGACGTCGAGGAGGTAAGTTCTGCTGATTCTTCTCTTTTATCTTATAGTGATTCTGAGCATTCGGTTATTACCTCTGACACCGTAGAAACCTTAATGGTTTATGACACTGAGTATGTGAATTATCTTGAATTATTATATAATCCTGAGAATTTGAATATTGCTTCTGTCAATTCTGTTCAGGAGGTTGGGGCTCCTGAACTAGACTCTCAAGTGAATACTGCTAATCTTCCTGCTACTGACTTGTCTGTGATGCCTTTCCATAGCCCCAAGAACAGGGGTAGGGACTATCTGGAGAACCTCACCACAGAACCCTCCTCTTCTCATTTACCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

239

Amino Acids

26.34

Weight (kDa)

4.55

Isoelectric Point (pI)

48.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 390
AccB7I CCANNNNNTGG 1 cut(s) 244
AciI CCGC 2 cut(s) 41, 118
AcsI RAATTY 1 cut(s) 532
AcyI GRCGYC 1 cut(s) 387
AfiI CCNNNNNNNGG 2 cut(s) 244, 657
AgsI TTSAA 3 cut(s) 374, 512, 538
AlwNI CAGNNNCTG 1 cut(s) 62
AoxI GGCC 1 cut(s) 14
ApeKI GCWGC 2 cut(s) 56, 254
ApoI RAATTY 1 cut(s) 532
AspS9I GGNCC 1 cut(s) 38
AsuHPI GGTGA 3 cut(s) 182, 308, 676
AvaII GGWCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 577
BauI CACGAG 1 cut(s) 84
BbvI GCAGC 2 cut(s) 68, 241
BccI CCATC 1 cut(s) 201
BfaI CTAG 5 cut(s) 24, 123, 225, 347, 584
BfmI CTRYAG 1 cut(s) 54
BisI GCNGC 3 cut(s) 57, 118, 255
BlsI GCNGC 3 cut(s) 58, 119, 256
Bme18I GGWCC 1 cut(s) 38
BmgT120I GGNCC 1 cut(s) 38
BmiI GGNNCC 4 cut(s) 39, 219, 264, 576
BmsI GCATC 2 cut(s) 130, 624
BpmI CTGGAG 1 cut(s) 695
Bpu10I CCTNAGC 2 cut(s) 258, 267
BpuEI CTTGAG 1 cut(s) 576
BsaHI GRCGYC 1 cut(s) 387
BsaJI CCNNGG 1 cut(s) 213
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 657
BseDI CCNNGG 1 cut(s) 213
BseLI CCNNNNNNNGG 2 cut(s) 244, 657
BseMII CTCAG 3 cut(s) 429, 483, 519
BseRI GAGGAG 3 cut(s) 90, 407, 688
BseXI GCAGC 2 cut(s) 68, 241
BshFI GGCC 1 cut(s) 16
BslFI GGGAC 1 cut(s) 680
BslI CCNNNNNNNGG 2 cut(s) 244, 657
BsmFI GGGAC 1 cut(s) 680
BsmI GAATGC 1 cut(s) 442
BsnI GGCC 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 577
Bsp143I GATC 2 cut(s) 304, 349
BspACI CCGC 2 cut(s) 41, 118
BspANI GGCC 1 cut(s) 16
BspCNI CTCAG 3 cut(s) 430, 484, 520
BspLI GGNNCC 4 cut(s) 39, 219, 264, 576
BspMAI CTGCAG 1 cut(s) 58
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 2 cut(s) 304, 349
BssNI GRCGYC 1 cut(s) 387
BssSI CACGAG 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 213
Bst2BI CACGAG 1 cut(s) 84
Bst4CI ACNGT 2 cut(s) 166, 466
Bst6I CTCTTC 1 cut(s) 706
BstACI GRCGYC 1 cut(s) 387
BstAPI GCANNNNNTGC 1 cut(s) 62
BstDEI CTNAG 5 cut(s) 258, 267, 438, 492, 528
BstKTI GATC 2 cut(s) 307, 352
BstMBI GATC 2 cut(s) 304, 349
BstMWI GCNNNNNNNGC 2 cut(s) 62, 123
BstSFI CTRYAG 1 cut(s) 54
BstV1I GCAGC 2 cut(s) 68, 241
BsuRI GGCC 1 cut(s) 16
BtsIMutI CAGTG 2 cut(s) 162, 489
CaiI CAGNNNCTG 1 cut(s) 62
Cfr13I GGNCC 1 cut(s) 38
CseI GACGC 1 cut(s) 115
CviAII CATG 2 cut(s) 158, 197
CviJI RGCY 6 cut(s) 16, 59, 208, 271, 575, 648
CviKI_1 RGCY 6 cut(s) 16, 59, 208, 271, 575, 648
DdeI CTNAG 5 cut(s) 258, 267, 438, 492, 528
DpnI GATC 2 cut(s) 306, 351
DpnII GATC 2 cut(s) 304, 349
DraI TTTAAA 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 706
EarI CTCTTC 1 cut(s) 706
EciI GGCGGA 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 213
Eco24I GRGCYC 1 cut(s) 577
Eco47I GGWCC 1 cut(s) 38
EcoT14I CCWWGG 1 cut(s) 213
EcoT38I GRGCYC 1 cut(s) 577
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 2 cut(s) 161, 200
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 1 cut(s) 680
FatI CATG 2 cut(s) 157, 196
Fnu4HI GCNGC 3 cut(s) 57, 118, 255
FriOI GRGCYC 1 cut(s) 577
Fsp4HI GCNGC 3 cut(s) 57, 118, 255
FspBI CTAG 5 cut(s) 24, 123, 225, 347, 584
GluI GCNGC 3 cut(s) 57, 118, 255
GsuI CTGGAG 1 cut(s) 695
HaeIII GGCC 1 cut(s) 16
HgaI GACGC 1 cut(s) 115
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 2 cut(s) 161, 200
HincII GTYRAC 2 cut(s) 175, 385
HindII GTYRAC 2 cut(s) 175, 385
HinfI GANTC 5 cut(s) 20, 299, 410, 434, 587
HphI GGTGA 3 cut(s) 182, 308, 676
Hpy166II GTNNAC 3 cut(s) 175, 190, 385
Hpy188I TCNGA 3 cut(s) 304, 439, 460
Hpy188III TCNNGA 7 cut(s) 24, 353, 509, 527, 563, 578, 674
Hpy8I GTNNAC 3 cut(s) 175, 190, 385
Hpy99I CGWCG 1 cut(s) 392
HpyCH4III ACNGT 2 cut(s) 166, 466
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 3 cut(s) 56, 157, 200
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 123
HpyF3I CTNAG 5 cut(s) 258, 267, 438, 492, 528
HpySE526I ACGT 1 cut(s) 387
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 2 cut(s) 161, 200
Kzo9I GATC 2 cut(s) 304, 349
LmnI GCTCC 2 cut(s) 103, 580
Lsp1109I GCAGC 2 cut(s) 68, 241
LweI GCATC 2 cut(s) 130, 624
MaeI CTAG 5 cut(s) 24, 123, 225, 347, 584
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 1 cut(s) 127
MalI GATC 2 cut(s) 306, 351
MboI GATC 2 cut(s) 304, 349
MboII GAAGA 5 cut(s) 386, 389, 405, 602, 693
MhlI GDGCHC 1 cut(s) 577
MluCI AATT 7 cut(s) 109, 151, 178, 502, 512, 532, 553
MlyI GAGTC 2 cut(s) 308, 581
MseI TTAA 6 cut(s) 11, 30, 77, 108, 315, 476
MslI CAYNNNNRTG 1 cut(s) 195
Mva1269I GAATGC 1 cut(s) 442
MwoI GCNNNNNNNGC 2 cut(s) 62, 123
NdeII GATC 2 cut(s) 304, 349
NlaIII CATG 2 cut(s) 161, 200
NlaIV GGNNCC 4 cut(s) 39, 219, 264, 576
NmuCI GTSAC 1 cut(s) 127
PctI GAATGC 1 cut(s) 442
PfeI GAWTC 3 cut(s) 20, 410, 434
PflMI CCANNNNNTGG 1 cut(s) 244
PkrI GCNGC 3 cut(s) 58, 119, 256
PleI GAGTC 2 cut(s) 307, 581
PpsI GAGTC 2 cut(s) 307, 581
PspN4I GGNNCC 4 cut(s) 39, 219, 264, 576
PspPI GGNCC 1 cut(s) 38
PstI CTGCAG 1 cut(s) 58
PstNI CAGNNNCTG 1 cut(s) 62
RseI CAYNNNNRTG 1 cut(s) 195
SaqAI TTAA 6 cut(s) 11, 30, 77, 108, 315, 476
SatI GCNGC 3 cut(s) 57, 118, 255
Sau3AI GATC 2 cut(s) 304, 349
Sau96I GGNCC 1 cut(s) 38
SchI GAGTC 2 cut(s) 308, 581
SduI GDGCHC 1 cut(s) 577
SetI ASST 9 cut(s) 268, 330, 390, 399, 458, 476, 570, 684, 715
SfaNI GCATC 2 cut(s) 130, 624
SfcI CTRYAG 1 cut(s) 54
SinI GGWCC 1 cut(s) 38
SmiMI CAYNNNNRTG 1 cut(s) 195
SmlI CTYRAG 1 cut(s) 591
SmoI CTYRAG 1 cut(s) 591
Sse9I AATT 7 cut(s) 109, 151, 178, 502, 512, 532, 553
SsiI CCGC 2 cut(s) 41, 118
SspI AATATT 1 cut(s) 541
SspMI CTAG 5 cut(s) 24, 123, 225, 347, 584
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 2 cut(s) 166, 466
TaiI ACGT 1 cut(s) 390
TaqI TCGA 1 cut(s) 390
TasI AATT 7 cut(s) 109, 151, 178, 502, 512, 532, 553
TauI GCSGC 1 cut(s) 120
TfiI GAWTC 3 cut(s) 20, 410, 434
Tru1I TTAA 6 cut(s) 11, 30, 77, 108, 315, 476
Tru9I TTAA 6 cut(s) 11, 30, 77, 108, 315, 476
TscAI CASTG 2 cut(s) 169, 496
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 2 cut(s) 56, 254
Tsp45I GTSAC 1 cut(s) 127
TspRI CASTG 2 cut(s) 169, 496
Van91I CCANNNNNTGG 1 cut(s) 244
VpaK11BI GGWCC 1 cut(s) 38
XapI RAATTY 1 cut(s) 532
XbaI TCTAGA 1 cut(s) 23
XspI CTAG 5 cut(s) 24, 123, 225, 347, 584
ZraI GACGTC 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.