AT1G09620

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000598
Physical Location & Seq
Reverse (-)
3112778 .. 3116699
3922 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G09620.1

Sequence Viewer

Length: 3276 bp
ATGGCATCGGAATCGAAAAGCTACGCTAGGAGAGATCGTCTTCTTGAGATTGAGGCCACTGTTCGAAAGTGGTGGGAAGACGAGGATGTATTCAGAGCCGAATCTTGTGAGAATCTTCCAAAACCAGGAGAAAAGTTTTTCTCAACCTTCCCTTTCCCGTATATGAATGGTTATCTACACATTGGGCATGCCTTTTCATTGTCCAAGGTTGATTTTGCTTCTGCTTACCATAGGCTAAGAGGAGCTAATGTGCTTCTTCCATTTGGTTTCCATTGTACTGGTATGCCAATTAAGGCTTCTGCGGATAAGCTTCGTCGTGAGATTGAACAGTTTGGTAACCCTCCTGTGTTTACTGCTGAAGACACCACCAAAGTTCCTGAAGTTCAGGAGGAGAGCAGTGATACTATAGCTTTACCGATTCCGGGTCAGTTCAAGGGAAAGAAGTCTAAGGTTGCTGCCAAAGCTGGTGGACAGGTTTATCAGTGGGAGATTATGCGCAGTTTTGGTCTTACTGACAGTGAGATTGCAAATTTCCGAGAGCCTTCTGAATGGTTGTATTACTTTCCCCCTTTGGCTGTTGAAGATCTAAGGGCATATGGATTGGGTTGTGATTGGAGACGCTCGTTTGTGACCACTGATGTTAATCCCTTTTTCGATGCCTTTGTGAGGTGGCAGATGAGGAAGTTGAAATCTATGGGAAAGATTGTTAAAGACCGCAGGTACACTATATTCTCACCTTTAGATGGCCAGCCTTGTGCTGATCACGACCGTGCTACTGGGGAAGGTGTTCAGCCTCAAGAGTATACACTCATAAAGATGGAAGTAGTCAAGCCATTCCCTCTGAAACTGGGTCCTTTGGAAGGCAAGAGAGTGTTTTTGGCTGCAGCTACTTTGAGGCCTGAGACCATGTATGGACAAACAAACGCATGGGTACTACCTGATGGGAAATATGGAGCTTATGAAATCAGCGAAACTGAGGTCTTCATCCTTACTGAGAGAGCTGCGCTCAACCTTGCGTACCAGAACTTTTCGAAGAACCCTCAGGAGCCTTCCTGCTTGGTTGAGTTGACCGGGTATGATCTGATTGGACTCCCTTTGAGGTCTCCTCTGTCAGTCAACGAGATCATCTATGCTCTGCCCATGTTGACCATTCTGACCAACAAAGGTACTGGCATTGTCACCAGTGTGCCTAGTGATGCTCCTGATGATTACATGGCTTTACAAGATTTAATCAAAAAGCCTGCTCTTCAAGATAAGTATGGTGTGAAAACGGAATGGTTGCCCACTGAAATCATACCGATCATCAACATTCCGGAATTTGGGGATAAAGCTGCTGAAAAGGTCTGCTTGGATCTGAAAATTAAAAGCCAAAACGATAAGGAAAAGCTTGCAGAGGCTAAGAGGTTGACTTACCTGAAAGGATTTACCGAAGGAACCATGCTTATTGGAGAGTTTTTTGGTAGGAAAGTTCAAGAAATTAAGCCCATTATCAAGACAAAGCTCATAGAGACTGGCGAGGCAATCATATACAGTGAACCCGAGAAGCCGGTGATGTCAAGATCGGGTGATGAATGTGTTGTGGCTCTTACAGATCAGTGGTACATAACATACGGCGAATCAGAATGGCGGAAAATTGCTGAGGAATGCCTATCAAAGATGAATCTCTACTCTGATGAAACAAGGCATGGCTTTGAGCACACTTTGAGCTGGCTCAATCAGTGGGCTTGCTCACGATCTTTTGGTTTAGGAACTCGTATTCCCTGGGATGAACAGTTCCTAGTGGAATCCTTATCTGATTCATCTCTTTATATGGCCTATTACACAGTTGCCCATATCTTTCACGATGGAGACATGTATAAAGGTAGCAAGTCTCTGATCCGCCCTCAGCAGATGAATGATGAAGTTTGGGAGTATCTCTTCTGTGATGGCCCGTACCCTAAATCATCTGATATTCCATCAGCTGTCTTAAGTGAGATGAAGCAGGAATTTGACTACTGGTACCCGCTAGATCTTCGAGTTTCAGGAAAGGATCTTATTCAGAATCATTTGACATTTTTCATCTACAACCACACTGCACTAATGGCGAATCGTAACTGGCCACGTGGGATCAGATGTAATGGTCATATCATGCTGAATTCTGAAAAGATGTCAAAGTCTACTGGAAATTTCAGAACCCTGCGCCAGTCTATTGAAGAATTCTCTGCCACTGGTACAAGGTTTTGTTTGGCTGATGCTGGTGATGGTGTTGATGATGCAAATTTTGCATTTGAAACTGCAAATGCTGCAATTTTGCGGTTGACAAAAGAGCTCACATGGATGGAAGAAGTTCTGGATGTCGAATCCTCTTTAAGAACGGGACCTCCATCTACATATGCTGATAAAGTGTTTGAAAATGACATGAACATTGCTCTCAGATTGACTGAAAGAGCGTACAAAGATTGTCTGTTTAGGGAGGCACTTAAAAATGGGTTTTACGACTTACAAGCTGCTCGGGATGAGTATAGACTCTCTTGTGGAACTGGAGGCATGCACCATGACTTGCTACTGAAATTTATGGATGTGCAAACACGCCTCATTGTACCAATCTGTCCCCATTTTGCAGATTATGTTTGGAGGAAAGTTTTGAATAAGGAAGGTTGTGTGCTCACAGCAGGCTGGCCACCATCAAATGAGCCAGATTTGGTGCTCAAGAGTGCAAACAAATATTTGCAAGATTCTATTGTGTTAATGAGAAAGCTTCTTCAAAAACAACTCTCAGGTTCCAAGAAGGGTGCTAAGAAAGGTGCTCAAGTAACAGCTGTGCCAGAGGGGAAGCTAAAAGGCCTAGTATATGTGAATGAGCAATTTGATGGATGGAGAGCTCACTGCCTGCGGATTCTGCAAAGTAGATTTGACCAGCAAACCTGTAGTTTCCCCCCGGATACAGAGATGCTTGCAGAACTAAGTGCAACATTGTTGCAGGAGGGAAAAAACTTGAAAGCCATTCAAAAGGTTTGCATGCCTTTCCTTAAATTCAAGAAGGACGAGGCAATATCTATTGGCACTCAGGCTCTGAACTTGAGGTTACCTTTTGGAGAGATCGAAGTCCTTCAGAGTAACAAGGACTTGATCAGGCGGCAACTTGGTCTTGAAGAGGTTGAAATATATTCTGCAAGTGACCCTGATGATGTTTCAATAGCTGGTCCACATGCTTCGCTGCTGACGCAGAATCCTCCATCTCCAGGCAGCCCAACTGCCATCTTTGTGACCAGCACAAGTGTCTGCCCTCCATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1091

Amino Acids

123.45

Weight (kDa)

5.74

Isoelectric Point (pI)

50.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 22 - 103 4.2e-11 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 49 - 111 3.1e-06 tRNA synthetases class I (M)
tRNA-synt_1 PF00133 191 - 753 2.9e-13 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1e PF01406 667 - 743 2.8e-06 tRNA synthetases class I (C) catalytic domain
tRNA-synt_1g PF09334 671 - 760 4.2e-08 tRNA synthetases class I (M)
Anticodon_1 PF08264 793 - 916 2.6e-10 Anticodon-binding domain of tRNA ligase
RBD_LARS1 PF24810 946 - 1022 3.9e-06 Leucine--tRNA ligase, RagD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 497
Acc36I ACCTGC 1 cut(s) 708
Acc65I GGTACC 1 cut(s) 1998
AccB1I GGYRCC 1 cut(s) 1998
AccI GTMKAC 2 cut(s) 803, 2156
AccIII TCCGGA 1 cut(s) 1312
AciI CCGC 8 cut(s) 302, 715, 1627, 1879, 2003, 2293, 2872, 3115
AclWI GGATC 4 cut(s) 1359, 1870, 2037, 2114
AcoI YGGCCR 3 cut(s) 745, 2096, 2657
AcsI RAATTY 9 cut(s) 529, 1316, 1985, 2134, 2164, 2195, 2257, 2549, 3011
AcuI CTGAAG 3 cut(s) 378, 399, 3074
AcvI CACGTG 1 cut(s) 2102
AfiI CCNNNNNNNGG 7 cut(s) 125, 422, 666, 743, 860, 1319, 3221
AflII CTTAAG 1 cut(s) 1966
AflIII ACRYGT 1 cut(s) 1851
AjnI CCWGG 3 cut(s) 124, 1760, 3220
AjuI GAANNNNNNNTTGG 2 cut(s) 280, 312
AleI CACNNNNGTG 2 cut(s) 768, 1184
AloI GAACNNNNNNTCC 6 cut(s) 1741, 1757, 1773, 1789, 2344, 2376
Alw21I GWGCWC 6 cut(s) 1698, 2310, 2646, 2688, 2788, 2863
Alw26I GTCTC 6 cut(s) 610, 896, 1107, 1502, 1842, 1875
AlwI GGATC 4 cut(s) 1359, 1870, 2037, 2114
AlwNI CAGNNNCTG 1 cut(s) 3052
Ama87I CYCGRG 2 cut(s) 1538, 2490
Aor13HI TCCGGA 1 cut(s) 1312
AoxI GGCC 8 cut(s) 54, 745, 896, 1812, 1927, 2096, 2657, 2821
ApeKI GCWGC 9 cut(s) 455, 881, 884, 1001, 1331, 2282, 2486, 3196, 3225
ApoI RAATTY 9 cut(s) 529, 1316, 1985, 2134, 2164, 2195, 2257, 2549, 3011
ArsI GACNNNNNNTTYG 2 cut(s) 963, 995
Asp700I GAANNNNTTC 3 cut(s) 786, 2325, 3087
Asp718I GGTACC 1 cut(s) 1998
AspLEI GCGC 3 cut(s) 498, 1006, 2181
AspS9I GGNCC 4 cut(s) 851, 1928, 2357, 3182
AsuC2I CCSGG 3 cut(s) 423, 1072, 2918
AsuHPI GGTGA 5 cut(s) 726, 1171, 1561, 1577, 2249
AsuII TTCGAA 2 cut(s) 64, 1031
AvaI CYCGRG 2 cut(s) 1538, 2490
AvaII GGWCC 3 cut(s) 851, 2357, 3182
AxyI CCTNAGG 1 cut(s) 1041
BalI TGGCCA 3 cut(s) 747, 2098, 2659
BanI GGYRCC 1 cut(s) 1998
BanII GRGCYC 2 cut(s) 2310, 2863
BbrPI CACGTG 1 cut(s) 2102
BbsI GAAGAC 4 cut(s) 32, 84, 366, 973
Bbv12I GWGCWC 6 cut(s) 1698, 2310, 2646, 2688, 2788, 2863
BbvCI CCTCAGC 2 cut(s) 1638, 1884
BbvI GCAGC 9 cut(s) 442, 868, 896, 988, 1318, 2269, 2473, 3183, 3237
BceAI ACGGC 1 cut(s) 1627
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 3 cut(s) 126, 1762, 3222
BciVI GTATCC 1 cut(s) 2914
BclI TGATCA 2 cut(s) 760, 3108
BcnI CCSGG 3 cut(s) 423, 1072, 2918
BcoDI GTCTC 6 cut(s) 610, 896, 1107, 1502, 1842, 1875
BfaI CTAG 5 cut(s) 27, 1191, 1778, 2006, 2825
BfmI CTRYAG 3 cut(s) 405, 882, 2905
BfrI CTTAAG 1 cut(s) 1966
BfuAI ACCTGC 1 cut(s) 708
BfuI GTATCC 1 cut(s) 2914
BglII AGATCT 2 cut(s) 583, 2008
Bme1390I CCNGG 6 cut(s) 126, 423, 1072, 1762, 2918, 3222
Bme18I GGWCC 3 cut(s) 851, 2357, 3182
BmeT110I CYCGRG 2 cut(s) 1538, 2490
BmgT120I GGNCC 4 cut(s) 851, 1928, 2357, 3182
BmiI GGNNCC 6 cut(s) 852, 1047, 1435, 2000, 2358, 2761
BmrFI CCNGG 6 cut(s) 126, 423, 1072, 1762, 2918, 3222
BmrI ACTGGG 2 cut(s) 786, 857
BmsI GCATC 6 cut(s) 14, 646, 1186, 2221, 2242, 2919
BmuI ACTGGG 2 cut(s) 786, 857
BpiI GAAGAC 4 cut(s) 32, 84, 366, 973
BplI GAGNNNNNCTC 4 cut(s) 990, 1022, 1090, 1122
BpmI CTGGAG 2 cut(s) 2541, 3204
Bpu10I CCTNAGC 2 cut(s) 1638, 1884
Bpu14I TTCGAA 2 cut(s) 64, 1031
BpuEI CTTGAG 5 cut(s) 65, 780, 2672, 2772, 3079
BpuMI CCSGG 3 cut(s) 423, 1072, 2918
BsaAI YACGTR 1 cut(s) 2102
BsaBI GATNNNNATC 1 cut(s) 642
BsaI GGTCTC 2 cut(s) 896, 1107
BsaJI CCNNGG 4 cut(s) 204, 1760, 1761, 2916
BsaWI WCCGGW 1 cut(s) 1312
BsaXI ACNNNNNCTCC 6 cut(s) 22, 52, 234, 264, 2344, 2374
Bsc4I CCNNNNNNNGG 7 cut(s) 125, 422, 666, 743, 860, 1319, 3221
Bse118I RCCGGY 1 cut(s) 1546
Bse21I CCTNAGG 1 cut(s) 1041
Bse3DI GCAATG 1 cut(s) 2403
Bse8I GATNNNNATC 1 cut(s) 642
BseAI TCCGGA 1 cut(s) 1312
BseBI CCWGG 3 cut(s) 126, 1762, 3222
BseDI CCNNGG 4 cut(s) 204, 1760, 1761, 2916
BseGI GGATG 9 cut(s) 91, 984, 1771, 2322, 2338, 2500, 2563, 2858, 3269
BseJI GATNNNNATC 1 cut(s) 642
BseLI CCNNNNNNNGG 7 cut(s) 125, 422, 666, 743, 860, 1319, 3221
BseMI GCAATG 1 cut(s) 2403
BseMII CTCAG 9 cut(s) 891, 966, 984, 1055, 1629, 1898, 2425, 2769, 3059
BseRI GAGGAG 3 cut(s) 255, 404, 1095
BseXI GCAGC 9 cut(s) 442, 868, 896, 988, 1318, 2269, 2473, 3183, 3237
BsgI GTGCAG 1 cut(s) 2058
Bsh1285I CGRYCG 1 cut(s) 769
BshFI GGCC 8 cut(s) 56, 747, 898, 1814, 1929, 2098, 2659, 2823
BshNI GGYRCC 1 cut(s) 1998
BsiEI CGRYCG 1 cut(s) 769
BsiHKAI GWGCWC 6 cut(s) 1698, 2310, 2646, 2688, 2788, 2863
BsiHKCI CYCGRG 2 cut(s) 1538, 2490
BsiSI CCGG 5 cut(s) 422, 1071, 1313, 1547, 2918
BslFI GGGAC 2 cut(s) 2370, 2574
BslI CCNNNNNNNGG 7 cut(s) 125, 422, 666, 743, 860, 1319, 3221
BsmAI GTCTC 6 cut(s) 610, 896, 1107, 1502, 1842, 1875
BsmBI CGTCTC 1 cut(s) 610
BsmFI GGGAC 2 cut(s) 2370, 2574
BsmI GAATGC 1 cut(s) 1649
BsnI GGCC 8 cut(s) 56, 747, 898, 1814, 1929, 2098, 2659, 2823
Bso31I GGTCTC 2 cut(s) 896, 1107
BsoBI CYCGRG 2 cut(s) 1538, 2490
Bsp119I TTCGAA 2 cut(s) 64, 1031
Bsp1286I GDGCHC 6 cut(s) 1698, 2310, 2646, 2688, 2788, 2863
Bsp13I TCCGGA 1 cut(s) 1312
BspACI CCGC 8 cut(s) 302, 715, 1627, 1879, 2003, 2293, 2872, 3115
BspANI GGCC 8 cut(s) 56, 747, 898, 1814, 1929, 2098, 2659, 2823
BspCNI CTCAG 9 cut(s) 892, 967, 985, 1054, 1630, 1897, 2424, 2768, 3058
BspEI TCCGGA 1 cut(s) 1312
BspLI GGNNCC 6 cut(s) 852, 1047, 1435, 2000, 2358, 2761
BspMAI CTGCAG 1 cut(s) 886
BspMI ACCTGC 1 cut(s) 708
BspPI GGATC 4 cut(s) 1359, 1870, 2037, 2114
BspQI GCTCTTC 1 cut(s) 1251
BspT104I TTCGAA 2 cut(s) 64, 1031
BspT107I GGYRCC 1 cut(s) 1998
BspTI CTTAAG 1 cut(s) 1966
BspTNI GGTCTC 2 cut(s) 896, 1107
BsrDI GCAATG 1 cut(s) 2403
BsrFI RCCGGY 1 cut(s) 1546
BssAI RCCGGY 1 cut(s) 1546
BssECI CCNNGG 4 cut(s) 204, 1760, 1761, 2916
BssNAI GTATAC 1 cut(s) 804
BssT1I CCWWGG 1 cut(s) 204
Bst1107I GTATAC 1 cut(s) 804
Bst2UI CCWGG 3 cut(s) 126, 1762, 3222
Bst4CI ACNGT 7 cut(s) 61, 330, 518, 770, 1532, 1773, 1825
Bst6I CTCTTC 3 cut(s) 1251, 1922, 3126
BstAFI CTTAAG 1 cut(s) 1966
BstAPI GCANNNNNTGC 2 cut(s) 2261, 2282
BstBAI YACGTR 1 cut(s) 2102
BstBI TTCGAA 2 cut(s) 64, 1031
BstEII GGTNACC 2 cut(s) 335, 3063
BstENI CCTNNNNNAGG 2 cut(s) 664, 858
BstF5I GGATG 9 cut(s) 91, 984, 1771, 2322, 2338, 2500, 2563, 2858, 3269
BstHHI GCGC 3 cut(s) 498, 1006, 2181
BstMAI GTCTC 6 cut(s) 610, 896, 1107, 1502, 1842, 1875
BstMCI CGRYCG 1 cut(s) 769
BstMWI GCNNNNNNNGC 6 cut(s) 461, 2081, 2261, 2282, 2878, 3202
BstNI CCWGG 3 cut(s) 126, 1762, 3222
BstNSI RCATGY 5 cut(s) 191, 1855, 2530, 3001, 3191
BstPI GGTNACC 2 cut(s) 335, 3063
BstSCI CCNGG 6 cut(s) 124, 421, 1070, 1760, 2916, 3220
BstSFI CTRYAG 3 cut(s) 405, 882, 2905
BstV1I GCAGC 9 cut(s) 442, 868, 896, 988, 1318, 2269, 2473, 3183, 3237
BstV2I GAAGAC 4 cut(s) 32, 84, 366, 973
BstX2I RGATCY 4 cut(s) 583, 1351, 2008, 2029
BstXI CCANNNNNNTGG 1 cut(s) 278
BstYI RGATCY 4 cut(s) 583, 1351, 2008, 2029
BstZ17I GTATAC 1 cut(s) 804
Bsu36I CCTNAGG 1 cut(s) 1041
BsuI GTATCC 1 cut(s) 2914
BsuRI GGCC 8 cut(s) 56, 747, 898, 1814, 1929, 2098, 2659, 2823
BtsCI GGATG 9 cut(s) 91, 984, 1771, 2322, 2338, 2500, 2563, 2858, 3269
BtsI GCAGTG 3 cut(s) 403, 2070, 2863
BveI ACCTGC 1 cut(s) 708
CaiI CAGNNNCTG 1 cut(s) 3052
CfoI GCGC 3 cut(s) 498, 1006, 2181
Cfr10I RCCGGY 1 cut(s) 1546
Cfr13I GGNCC 4 cut(s) 851, 1928, 2357, 3182
CseI GACGC 2 cut(s) 627, 3211
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
EaeI YGGCCR 3 cut(s) 745, 2096, 2657
Eam1104I CTCTTC 3 cut(s) 1251, 1922, 3126
EarI CTCTTC 3 cut(s) 1251, 1922, 3126
EciI GGCGGA 2 cut(s) 1642, 1868
Ecl136II GAGCTC 2 cut(s) 2308, 2861
Eco130I CCWWGG 1 cut(s) 204
Eco147I AGGCCT 2 cut(s) 898, 2823
Eco24I GRGCYC 2 cut(s) 2310, 2863
Eco31I GGTCTC 2 cut(s) 896, 1107
Eco47I GGWCC 3 cut(s) 851, 2357, 3182
Eco53kI GAGCTC 2 cut(s) 2308, 2861
Eco57I CTGAAG 3 cut(s) 378, 399, 3074
Eco72I CACGTG 1 cut(s) 2102
Eco81I CCTNAGG 1 cut(s) 1041
Eco88I CYCGRG 2 cut(s) 1538, 2490
Eco91I GGTNACC 2 cut(s) 335, 3063
EcoICRI GAGCTC 2 cut(s) 2308, 2861
EcoNI CCTNNNNNAGG 2 cut(s) 664, 858
EcoO109I RGGNCCY 2 cut(s) 851, 2357
EcoO65I GGTNACC 2 cut(s) 335, 3063
EcoRI GAATTC 2 cut(s) 2134, 2195
EcoRII CCWGG 3 cut(s) 124, 1760, 3220
EcoT14I CCWWGG 1 cut(s) 204
EcoT38I GRGCYC 2 cut(s) 2310, 2863
ErhI CCWWGG 1 cut(s) 204
Esp3I CGTCTC 1 cut(s) 610
FalI AAGNNNNNCTT 2 cut(s) 1331, 1363
FaqI GGGAC 2 cut(s) 2370, 2574
FauI CCCGC 1 cut(s) 2010
FauNDI CATATG 2 cut(s) 595, 2371
FbaI TGATCA 2 cut(s) 760, 3108
FblI GTMKAC 2 cut(s) 803, 2156
FokI GGATG 9 cut(s) 98, 971, 1778, 2329, 2345, 2507, 2570, 2865, 3256
FriOI GRGCYC 2 cut(s) 2310, 2863
FspBI CTAG 5 cut(s) 27, 1191, 1778, 2006, 2825
FspI TGCGCA 1 cut(s) 497
GlaI GCGC 3 cut(s) 497, 1005, 2180
GsuI CTGGAG 2 cut(s) 2541, 3204
HaeIII GGCC 8 cut(s) 56, 747, 898, 1814, 1929, 2098, 2659, 2823
HapII CCGG 5 cut(s) 422, 1071, 1313, 1547, 2918
HgaI GACGC 2 cut(s) 627, 3211
HhaI GCGC 3 cut(s) 498, 1006, 2181
Hin6I GCGC 3 cut(s) 496, 1004, 2179
HinP1I GCGC 3 cut(s) 496, 1004, 2179
HincII GTYRAC 5 cut(s) 1068, 1117, 1146, 1407, 2298
HindII GTYRAC 5 cut(s) 1068, 1117, 1146, 1407, 2298
HindIII AAGCTT 3 cut(s) 308, 1385, 2735
HpaII CCGG 5 cut(s) 422, 1071, 1313, 1547, 2918
HphI GGTGA 5 cut(s) 726, 1171, 1561, 1577, 2249
Hpy99I CGWCG 1 cut(s) 318
HpyCH4III ACNGT 7 cut(s) 61, 330, 518, 770, 1532, 1773, 1825
HpyCH4IV ACGT 1 cut(s) 2101
HpyF10VI GCNNNNNNNGC 6 cut(s) 461, 2081, 2261, 2282, 2878, 3202
HpySE526I ACGT 1 cut(s) 2101
HspAI GCGC 3 cut(s) 496, 1004, 2179
Kpn2I TCCGGA 1 cut(s) 1312
KpnI GGTACC 1 cut(s) 2002
Ksp22I TGATCA 2 cut(s) 760, 3108
LguI GCTCTTC 1 cut(s) 1251
LmnI GCTCC 4 cut(s) 242, 953, 1045, 1204
Lsp1109I GCAGC 9 cut(s) 442, 868, 896, 988, 1318, 2269, 2473, 3183, 3237
LweI GCATC 6 cut(s) 14, 646, 1186, 2221, 2242, 2919
MaeI CTAG 5 cut(s) 27, 1191, 1778, 2006, 2825
MaeII ACGT 1 cut(s) 2101
MaeIII GTNAC 9 cut(s) 335, 628, 1177, 2090, 2791, 3063, 3095, 3155, 3244
MflI RGATCY 4 cut(s) 583, 1351, 2008, 2029
MhlI GDGCHC 6 cut(s) 1698, 2310, 2646, 2688, 2788, 2863
MlsI TGGCCA 3 cut(s) 747, 2098, 2659
MluNI TGGCCA 3 cut(s) 747, 2098, 2659
MlyI GAGTC 2 cut(s) 1083, 2499
Mox20I TGGCCA 3 cut(s) 747, 2098, 2659
MroI TCCGGA 1 cut(s) 1312
MroXI GAANNNNTTC 3 cut(s) 786, 2325, 3087
MscI TGGCCA 3 cut(s) 747, 2098, 2659
MslI CAYNNNNRTG 5 cut(s) 768, 815, 1184, 2315, 3242
Msp20I TGGCCA 3 cut(s) 747, 2098, 2659
MspA1I CMGCKG 2 cut(s) 1961, 2798
MspCI CTTAAG 1 cut(s) 1966
MspI CCGG 5 cut(s) 422, 1071, 1313, 1547, 2918
MspR9I CCNGG 6 cut(s) 126, 423, 1072, 1762, 2918, 3222
Mva1269I GAATGC 1 cut(s) 1649
MvaI CCWGG 3 cut(s) 126, 1762, 3222
MwoI GCNNNNNNNGC 6 cut(s) 461, 2081, 2261, 2282, 2878, 3202
NciI CCSGG 3 cut(s) 423, 1072, 2918
NdeI CATATG 2 cut(s) 595, 2371
NlaIV GGNNCC 6 cut(s) 852, 1047, 1435, 2000, 2358, 2761
NmuCI GTSAC 4 cut(s) 628, 1177, 3155, 3244
NsbI TGCGCA 1 cut(s) 497
NspI RCATGY 5 cut(s) 191, 1855, 2530, 3001, 3191
NspV TTCGAA 2 cut(s) 64, 1031
OliI CACNNNNGTG 2 cut(s) 768, 1184
PaeI GCATGC 3 cut(s) 191, 2530, 3001
PasI CCCWGGG 1 cut(s) 1761
PceI AGGCCT 2 cut(s) 898, 2823
PciI ACATGT 1 cut(s) 1851
PciSI GCTCTTC 1 cut(s) 1251
PctI GAATGC 1 cut(s) 1649
PdmI GAANNNNTTC 3 cut(s) 786, 2325, 3087
PleI GAGTC 2 cut(s) 1083, 2499
PmaCI CACGTG 1 cut(s) 2102
PmlI CACGTG 1 cut(s) 2102
PpsI GAGTC 2 cut(s) 1083, 2499
Ppu21I YACGTR 1 cut(s) 2102
PpuMI RGGWCCY 2 cut(s) 851, 2357
PscI ACATGT 1 cut(s) 1851
Psp124BI GAGCTC 2 cut(s) 2310, 2863
Psp5II RGGWCCY 2 cut(s) 851, 2357
Psp6I CCWGG 3 cut(s) 124, 1760, 3220
PspCI CACGTG 1 cut(s) 2102
PspEI GGTNACC 2 cut(s) 335, 3063
PspGI CCWGG 3 cut(s) 124, 1760, 3220
PspN4I GGNNCC 6 cut(s) 852, 1047, 1435, 2000, 2358, 2761
PspPI GGNCC 4 cut(s) 851, 1928, 2357, 3182
PspPPI RGGWCCY 2 cut(s) 851, 2357
PstI CTGCAG 1 cut(s) 886
PstNI CAGNNNCTG 1 cut(s) 3052
PsuI RGATCY 4 cut(s) 583, 1351, 2008, 2029
PvuII CAGCTG 2 cut(s) 1961, 2798
RseI CAYNNNNRTG 5 cut(s) 768, 815, 1184, 2315, 3242
SacI GAGCTC 2 cut(s) 2310, 2863
SapI GCTCTTC 1 cut(s) 1251
Sau96I GGNCC 4 cut(s) 851, 1928, 2357, 3182
SchI GAGTC 2 cut(s) 1083, 2499
ScrFI CCNGG 6 cut(s) 126, 423, 1072, 1762, 2918, 3222
SduI GDGCHC 6 cut(s) 1698, 2310, 2646, 2688, 2788, 2863
SfaNI GCATC 6 cut(s) 14, 646, 1186, 2221, 2242, 2919
SfcI CTRYAG 3 cut(s) 405, 882, 2905
SfuI TTCGAA 2 cut(s) 64, 1031
SinI GGWCC 3 cut(s) 851, 2357, 3182
SmiMI CAYNNNNRTG 5 cut(s) 768, 815, 1184, 2315, 3242
SmlI CTYRAG 6 cut(s) 44, 795, 1966, 2687, 2787, 3058
SmoI CTYRAG 6 cut(s) 44, 795, 1966, 2687, 2787, 3058
SphI GCATGC 3 cut(s) 191, 2530, 3001
SseBI AGGCCT 2 cut(s) 898, 2823
SsiI CCGC 8 cut(s) 302, 715, 1627, 1879, 2003, 2293, 2872, 3115
SspI AATATT 1 cut(s) 2705
SspMI CTAG 5 cut(s) 27, 1191, 1778, 2006, 2825
SstI GAGCTC 2 cut(s) 2310, 2863
StuI AGGCCT 2 cut(s) 898, 2823
StyD4I CCNGG 6 cut(s) 124, 421, 1070, 1760, 2916, 3220
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 7 cut(s) 61, 330, 518, 770, 1532, 1773, 1825
TaiI ACGT 1 cut(s) 2104
TaqI TCGA 7 cut(s) 14, 64, 654, 1031, 2014, 2337, 3081
TatI WGTACW 1 cut(s) 275
TauI GCSGC 1 cut(s) 3118
TseFI GTSAC 4 cut(s) 628, 1177, 3155, 3244
TseI GCWGC 9 cut(s) 455, 881, 884, 1001, 1331, 2282, 2486, 3196, 3225
Tsp45I GTSAC 4 cut(s) 628, 1177, 3155, 3244
TspGWI ACGGA 1 cut(s) 1286
Vha464I CTTAAG 1 cut(s) 1966
VpaK11BI GGWCC 3 cut(s) 851, 2357, 3182
XagI CCTNNNNNAGG 2 cut(s) 664, 858
XapI RAATTY 9 cut(s) 529, 1316, 1985, 2134, 2164, 2195, 2257, 2549, 3011
XceI RCATGY 5 cut(s) 191, 1855, 2530, 3001, 3191
XmiI GTMKAC 2 cut(s) 803, 2156
XmnI GAANNNNTTC 3 cut(s) 786, 2325, 3087
XspI CTAG 5 cut(s) 27, 1191, 1778, 2006, 2825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.