Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 91 |
| AccII | CGCG | 2 cut(s) | 152, 165 |
| AciI | CCGC | 4 cut(s) | 18, 89, 123, 168 |
| AclWI | GGATC | 2 cut(s) | 122, 350 |
| AcoI | YGGCCR | 1 cut(s) | 283 |
| AcyI | GRCGYC | 1 cut(s) | 75 |
| AfaI | GTAC | 2 cut(s) | 155, 331 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 111, 177 |
| AjiI | CACGTC | 1 cut(s) | 85 |
| AjnI | CCWGG | 1 cut(s) | 379 |
| AluBI | AGCT | 1 cut(s) | 196 |
| AluI | AGCT | 1 cut(s) | 196 |
| Alw21I | GWGCWC | 1 cut(s) | 84 |
| AlwI | GGATC | 2 cut(s) | 122, 350 |
| AoxI | GGCC | 3 cut(s) | 102, 283, 377 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 275 |
| AspLEI | GCGC | 2 cut(s) | 167, 227 |
| AspS9I | GGNCC | 2 cut(s) | 103, 136 |
| AsuHPI | GGTGA | 1 cut(s) | 230 |
| AvaII | GGWCC | 1 cut(s) | 136 |
| Bbv12I | GWGCWC | 1 cut(s) | 84 |
| BccI | CCATC | 1 cut(s) | 275 |
| BceAI | ACGGC | 3 cut(s) | 5, 124, 319 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 299, 333 |
| BciT130I | CCWGG | 1 cut(s) | 381 |
| BisI | GCNGC | 1 cut(s) | 168 |
| BlpI | GCTNAGC | 1 cut(s) | 92 |
| BlsI | GCNGC | 1 cut(s) | 169 |
| Bme1390I | CCNGG | 1 cut(s) | 381 |
| Bme18I | GGWCC | 1 cut(s) | 136 |
| BmgBI | CACGTC | 1 cut(s) | 85 |
| BmgT120I | GGNCC | 2 cut(s) | 103, 136 |
| BmiI | GGNNCC | 1 cut(s) | 214 |
| BmrFI | CCNGG | 1 cut(s) | 381 |
| BmsI | GCATC | 2 cut(s) | 45, 307 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 92 |
| BsaHI | GRCGYC | 1 cut(s) | 75 |
| BsaJI | CCNNGG | 4 cut(s) | 106, 126, 208, 253 |
| BsaWI | WCCGGW | 1 cut(s) | 215 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 53, 83 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 111, 177 |
| BseBI | CCWGG | 1 cut(s) | 381 |
| BseDI | CCNNGG | 4 cut(s) | 106, 126, 208, 253 |
| BseGI | GGATG | 1 cut(s) | 198 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 111, 177 |
| BseMII | CTCAG | 1 cut(s) | 106 |
| BseYI | CCCAGC | 1 cut(s) | 171 |
| Bsh1236I | CGCG | 2 cut(s) | 152, 165 |
| BshFI | GGCC | 3 cut(s) | 104, 285, 379 |
| BsiHKAI | GWGCWC | 1 cut(s) | 84 |
| BsiSI | CCGG | 4 cut(s) | 72, 216, 286, 294 |
| BslFI | GGGAC | 1 cut(s) | 71 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 111, 177 |
| BsmFI | GGGAC | 1 cut(s) | 71 |
| BsnI | GGCC | 3 cut(s) | 104, 285, 379 |
| Bsp1286I | GDGCHC | 1 cut(s) | 84 |
| Bsp143I | GATC | 3 cut(s) | 114, 342, 408 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 92 |
| BspACI | CCGC | 4 cut(s) | 18, 89, 123, 168 |
| BspANI | GGCC | 3 cut(s) | 104, 285, 379 |
| BspCNI | CTCAG | 1 cut(s) | 105 |
| BspFNI | CGCG | 2 cut(s) | 152, 165 |
| BspLI | GGNNCC | 1 cut(s) | 214 |
| BspPI | GGATC | 2 cut(s) | 122, 350 |
| BsrBI | CCGCTC | 1 cut(s) | 91 |
| BssECI | CCNNGG | 4 cut(s) | 106, 126, 208, 253 |
| BssMI | GATC | 3 cut(s) | 114, 342, 408 |
| BssNI | GRCGYC | 1 cut(s) | 75 |
| Bst2UI | CCWGG | 1 cut(s) | 381 |
| BstACI | GRCGYC | 1 cut(s) | 75 |
| BstC8I | GCNNGC | 1 cut(s) | 377 |
| BstDEI | CTNAG | 2 cut(s) | 92, 398 |
| BstDSI | CCRYGG | 1 cut(s) | 106 |
| BstF5I | GGATG | 1 cut(s) | 198 |
| BstFNI | CGCG | 2 cut(s) | 152, 165 |
| BstHHI | GCGC | 2 cut(s) | 167, 227 |
| BstKTI | GATC | 3 cut(s) | 117, 345, 411 |
| BstMBI | GATC | 3 cut(s) | 114, 342, 408 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 88, 303 |
| BstNI | CCWGG | 1 cut(s) | 381 |
| BstSCI | CCNGG | 1 cut(s) | 379 |
| BstUI | CGCG | 2 cut(s) | 152, 165 |
| BstX2I | RGATCY | 1 cut(s) | 342 |
| BstYI | RGATCY | 1 cut(s) | 342 |
| BsuRI | GGCC | 3 cut(s) | 104, 285, 379 |
| BtgI | CCRYGG | 1 cut(s) | 106 |
| BtrI | CACGTC | 1 cut(s) | 85 |
| BtsCI | GGATG | 1 cut(s) | 198 |
| BtsI | GCAGTG | 1 cut(s) | 243 |
| BtsIMutI | CAGTG | 2 cut(s) | 228, 243 |
| Cac8I | GCNNGC | 1 cut(s) | 377 |
| CfoI | GCGC | 2 cut(s) | 167, 227 |
| Cfr13I | GGNCC | 2 cut(s) | 103, 136 |
| CseI | GACGC | 2 cut(s) | 64, 164 |
| Csp6I | GTAC | 2 cut(s) | 154, 330 |
| CviAII | CATG | 2 cut(s) | 52, 339 |
| CviQI | GTAC | 2 cut(s) | 154, 330 |
| DdeI | CTNAG | 2 cut(s) | 92, 398 |
| DpnI | GATC | 3 cut(s) | 116, 344, 410 |
| DpnII | GATC | 3 cut(s) | 114, 342, 408 |
| EaeI | YGGCCR | 1 cut(s) | 283 |
| EciI | GGCGGA | 2 cut(s) | 7, 112 |
| Eco147I | AGGCCT | 1 cut(s) | 379 |
| Eco47I | GGWCC | 1 cut(s) | 136 |
| EcoRII | CCWGG | 1 cut(s) | 379 |
| FaeI | CATG | 2 cut(s) | 55, 342 |
| FaiI | YATR | 2 cut(s) | 53, 340 |
| FaqI | GGGAC | 1 cut(s) | 71 |
| FatI | CATG | 2 cut(s) | 51, 338 |
| FauI | CCCGC | 1 cut(s) | 96 |
| Fnu4HI | GCNGC | 1 cut(s) | 168 |
| FokI | GGATG | 1 cut(s) | 185 |
| Fsp4HI | GCNGC | 1 cut(s) | 168 |
| GlaI | GCGC | 2 cut(s) | 166, 226 |
| GluI | GCNGC | 1 cut(s) | 168 |
| GsaI | CCCAGC | 1 cut(s) | 175 |
| HaeIII | GGCC | 3 cut(s) | 104, 285, 379 |
| HapII | CCGG | 4 cut(s) | 72, 216, 286, 294 |
| HgaI | GACGC | 2 cut(s) | 64, 164 |
| HhaI | GCGC | 2 cut(s) | 167, 227 |
| Hin1I | GRCGYC | 1 cut(s) | 75 |
| Hin1II | CATG | 2 cut(s) | 55, 342 |
| Hin6I | GCGC | 2 cut(s) | 165, 225 |
| HinP1I | GCGC | 2 cut(s) | 165, 225 |
| HinfI | GANTC | 2 cut(s) | 119, 233 |
| HpaII | CCGG | 4 cut(s) | 72, 216, 286, 294 |
| HphI | GGTGA | 1 cut(s) | 230 |
| Hpy166II | GTNNAC | 2 cut(s) | 301, 386 |
| Hpy188I | TCNGA | 1 cut(s) | 270 |
| Hpy8I | GTNNAC | 2 cut(s) | 301, 386 |
| Hpy99I | CGWCG | 4 cut(s) | 26, 44, 80, 180 |
| HpyCH4IV | ACGT | 2 cut(s) | 84, 184 |
| HpyCH4V | TGCA | 3 cut(s) | 36, 348, 375 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 88, 303 |
| HpyF3I | CTNAG | 2 cut(s) | 92, 398 |
| HpySE526I | ACGT | 2 cut(s) | 84, 184 |
| Hsp92I | GRCGYC | 1 cut(s) | 75 |
| Hsp92II | CATG | 2 cut(s) | 55, 342 |
| HspAI | GCGC | 2 cut(s) | 165, 225 |
| Kzo9I | GATC | 3 cut(s) | 114, 342, 408 |
| LmnI | GCTCC | 1 cut(s) | 218 |
| LweI | GCATC | 2 cut(s) | 45, 307 |
| MaeII | ACGT | 2 cut(s) | 84, 184 |
| MaeIII | GTNAC | 1 cut(s) | 218 |
| MalI | GATC | 3 cut(s) | 116, 344, 410 |
| MbiI | CCGCTC | 1 cut(s) | 91 |
| MboI | GATC | 3 cut(s) | 114, 342, 408 |
| MboII | GAAGA | 1 cut(s) | 323 |
| MfeI | CAATTG | 1 cut(s) | 31 |
| MflI | RGATCY | 1 cut(s) | 342 |
| MhlI | GDGCHC | 1 cut(s) | 84 |
| MluCI | AATT | 1 cut(s) | 31 |
| MlyI | GAGTC | 2 cut(s) | 113, 227 |
| MmeI | TCCRAC | 1 cut(s) | 395 |
| MnlI | CCTC | 7 cut(s) | 25, 136, 149, 167, 212, 218, 263 |
| MroXI | GAANNNNTTC | 1 cut(s) | 275 |
| MslI | CAYNNNNRTG | 1 cut(s) | 279 |
| MspI | CCGG | 4 cut(s) | 72, 216, 286, 294 |
| MspR9I | CCNGG | 1 cut(s) | 381 |
| MunI | CAATTG | 1 cut(s) | 31 |
| MvaI | CCWGG | 1 cut(s) | 381 |
| MvnI | CGCG | 2 cut(s) | 152, 165 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 88, 303 |
| NdeII | GATC | 3 cut(s) | 114, 342, 408 |
| NlaIII | CATG | 2 cut(s) | 55, 342 |
| NlaIV | GGNNCC | 1 cut(s) | 214 |
| NmeAIII | GCCGAG | 2 cut(s) | 107, 189 |
| NmuCI | GTSAC | 1 cut(s) | 218 |
| PceI | AGGCCT | 1 cut(s) | 379 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 114 |
| PdmI | GAANNNNTTC | 1 cut(s) | 275 |
| PkrI | GCNGC | 1 cut(s) | 169 |
| PleI | GAGTC | 2 cut(s) | 113, 227 |
| PpsI | GAGTC | 2 cut(s) | 113, 227 |
| Psp6I | CCWGG | 1 cut(s) | 379 |
| PspFI | CCCAGC | 1 cut(s) | 171 |
| PspGI | CCWGG | 1 cut(s) | 379 |
| PspN4I | GGNNCC | 1 cut(s) | 214 |
| PspPI | GGNCC | 2 cut(s) | 103, 136 |
| PsuI | RGATCY | 1 cut(s) | 342 |
| RsaI | GTAC | 2 cut(s) | 155, 331 |
| RsaNI | GTAC | 2 cut(s) | 154, 330 |
| RseI | CAYNNNNRTG | 1 cut(s) | 279 |
| SatI | GCNGC | 1 cut(s) | 168 |
| Sau3AI | GATC | 3 cut(s) | 114, 342, 408 |
| Sau96I | GGNCC | 2 cut(s) | 103, 136 |
| SchI | GAGTC | 2 cut(s) | 113, 227 |
| ScrFI | CCNGG | 1 cut(s) | 381 |
| SduI | GDGCHC | 1 cut(s) | 84 |
| SetI | ASST | 5 cut(s) | 87, 159, 187, 198, 255 |
| SfaNI | GCATC | 2 cut(s) | 45, 307 |
| SinI | GGWCC | 1 cut(s) | 136 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 279 |
| Sse9I | AATT | 1 cut(s) | 31 |
| SseBI | AGGCCT | 1 cut(s) | 379 |
| SsiI | CCGC | 4 cut(s) | 18, 89, 123, 168 |
| StuI | AGGCCT | 1 cut(s) | 379 |
| StyD4I | CCNGG | 1 cut(s) | 379 |
| TaiI | ACGT | 2 cut(s) | 87, 187 |
| TaqI | TCGA | 4 cut(s) | 39, 78, 117, 279 |
| TasI | AATT | 1 cut(s) | 31 |
| TauI | GCSGC | 1 cut(s) | 170 |
| TscAI | CASTG | 2 cut(s) | 235, 250 |
| TseFI | GTSAC | 1 cut(s) | 218 |
| Tsp45I | GTSAC | 1 cut(s) | 218 |
| TspDTI | ATGAA | 1 cut(s) | 68 |
| TspRI | CASTG | 2 cut(s) | 235, 250 |
| VpaK11BI | GGWCC | 1 cut(s) | 136 |
| XmnI | GAANNNNTTC | 1 cut(s) | 275 |