MD08G1244600.v1.1

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000598
Physical Location & Seq
Forward (+)
30955665 .. 30961032
5368 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1244600.v1.1.491

Sequence Viewer

Length: 3294 bp
ATGGCGGCCGAAGGTGGTGGGAAGAGCTTTGCCAGGAGAGACCACCTTCTCGAGATTGAAACAAAGGTTCGAAGCTGGTGGGAAGAGAAGGATGTTTTCAGGGCTGAATCCTGTGACAAGCCCCCCGAACCTGGGGAGAAGTTTTTTGGAAACTTCCCGTTTCCTTACATGAATGGCTTTTTGCATCTTGGACATGCATTCTCGCTCTCCAAGCTAGAGTTTGCTGCAGCTTACCATAGGTTAAGAGGTGCCAATGTGCTTTTGCCTTTTGGTTTTCACTGCACTGGCATGCCTATTAAAGCGTCTGCTGATAAACTTGCGAGAGAGATTGAACTATTTGGCAACCCGCCGGTTTTTCCAAAAGAAGTAGAACAGGGGAGTCAGGAAGTGGAAGCAGAAGATGCAAATAATGGAGCACCTCCCGACAAGTTCAAGGGAAAGAAGTCAAAGGCTGCATCAAAATCAGGTGGACAAGTATACCAATGGGAAATCATGCGTAGTTTTGGCCTCTCAGATAGCGAGATATCCAAATTCCAAGATCCATACAATTGGTTGACATTCTTCCCTCCATTGGCAGTGGAAGACCTCAAGGCTTTTGGATTGGGATGTGACTGGAGACGGTCATTTATTACTACTGACATGAACCCATTTTTTGATGCCTTTGTGAGGTGGCAGATGAGGAAACTAAAATCTATGGGCAAGATTGTGAAAGATGTCCGTTATGCAATTTACTCTCCACTGGATGGGCAGCCCTGTGCAGATCATGACAGGGCAAGTGGCGAAGGAGTTCAGCCCCAAGAATACACCCTCATCAAAATGGAGGTGGTGGCACCTTTTCCATCTAAACTGAAAGTGTTGGAGGGAAAGAAAGTGTTCCTTGCTGCAGCAACACTTAGACCTGAGACCATGTATGGACAGACAAATGCATGGGTATTGCCTGATGGGAAATATGGAGCCTTCGAAATCAACGAAACAGAGGTATTTATTGTAACACAAAGAGCAGCACTTAATCTTGCCTATCAGAAGTATTCTAGGGTTCCAGAGAAACCTACTTGCTTAGTGGAGCTCACAGGTCATGATTTGATTGGCCTTCCATTGAAGTCACCACGCACAATCAATCCGATCATTTACACTCTTCCTATGTTGACTGTCCTGACAGACAAAGGTACTGGGATTGTGACTAGTGTGCCTGCTGATTCTCCTGATGATTATATGGCCTTGCATGATTTAAAAGCAAAACCTGCTCTTAGAGCAAAATATGGCGTGAAGGATGAGTGGGTCATGCCCTTTGAGATTATTCCAATTATTGATATTCCTGAATTTGGGAATAAGGCTGCGGAAAAGGTCTGTGCAGATCTAAAAATCAAGAGCCAGAATGAGAAAGAGAAGCTAGCAGAAGCCAAGAGGCTGACATACCTGAAAGGATTCACTGAAGGAACACTTATCGTGGGTGAATTCGAAGGAAGGAAAGTCCAGGAGGTGAAACCGTTGATAAGGAGCAAGCTCATAGAGTCAAATGAGGCAATTGTATATAGTGAACCTGAGAAGAGGGTGGTGTCACGATCAGGTGATGAGTGTGTTGTGGCTCTCACAGATCAATGGTACATCACATATGGGGAACCTGAATGGAAAAAACTGGCGGAGGAGTGCTTGTCCAGCATGAATTTGTATCATGACGAGACAAAACATGGATTTGAACACACATTGGATTGGTTGAATCAGTGGGCTTGTTCACGATCTTTTGGGCTTGGGACTCGTATTCCCTGGGATGAAGAGTTCTTAGTTGAGTCGTTGTCTGATTCAACTATTTACATGGCTTATTACACTATCGCTCATTTTTTACACAATGGAGACATGTATGGTCCCAGCAAATCTGCAATTCAACCTGAACAAATGACTGATGAGGTATGGGAGTATGTTTTCTGTGATGGCCCATATCCTGAATCATCTAACATATCGTCATCCATCCTTAATAAGATGAAGCAGGAGTTTGAGTATTGGTATCCTTTTGATCTTCGAGTGTCTGGCAAGGATCTAATCCAAAATCATTTAACCTTCTGCATTTACAACCACACGGCAATTATGCCCAAGAAGCACTGGCCTCGTGGCTTTAGATGCAATGGGCACATCATGCTAAATGCTGAGAAGATGTCTAAGTCCACAGGGAACTTTAGGACCATTCGCCAGGCAATTGAGGAATTTTCTGCTGATGCCACGCGATTCTCATTGGCTGATGCTGGAGATGGTGTTGATGATGCAAATTTCGTATTTGAGACTGCAAATGCTGCTATACTAAAGCTAACTAAAGAGATTGCATGGATGGAAGAAGTTTTAGCCGCAGAATCCTCTTTGAGAATAGGTCCTCCTTCTACATATGCTGACAGGGTTTTTTTGAATGAGATAAATATTGCTGTGAACAGGACTGAGCAGAATTACCGGGATTACATGTTCCGAGATGCACTCAAGACTGGGTTTTACGATCTTCAGGCTTCTAGGGATGAGTACAGGCTTTCATGTGGTTCTGGGGGCATGAACCGTGAATTGCTGTGGCATTTTATGGATGCGCAGACACGTCTTATTACTCCAATCTGTCCGCACTATGCAGAATATGTCTGGAGGGAACTTTTGAAGAAGGAAGGGTTTGTGGTCAATGCAGGATGGCCTGTGTCTGATTCTCCAGATCTGACCCTCCATAATGCCAACAAGTATTTGCAACATTCCATTGTTGTAATGAGGAAGCTTCTTGAAAAGCAAACTTCAGGCTCAACGAAAGCCAATAAGAAGGGTAATCCAGTTAAAGCATCGACAGAAACCAAAAGGTTGATTGGTTTAATATATGTAAATGAGCAATTTGATGGATGGAAGGCGGAGTGTTTGAGGATACTACAGAGTAACTTTGACAGTGATACTCGCACTTTTGCTCCAGACAAGGTAATTCTGGAGGCTTTGCAGAGTTGTTCAATTGGTCAGAGTAAAGATTTTAGACAAACCCAAAAGCTTTGTATGCCTTTCTTGAGGTTCAAGAAGGACGAGGCAGTTACTCTTGGGCCCCATGCCTTAGACTTGAAGCTACCATTCGGAGAGATGGAGGTCCTTCAGGAGAACTTGGACTTAATTAAGAGACAAGTGAAGGTGGACACTAAGGGACAGGGACAAATCGAGCTTGAAGAAGTAGAAGTTCTTTCTGGTACCGACCCTGGTGCTCTTGCTAAAGCTGGTTCACTTGCTAAATTAATAGAGCAGAATCCGCCATCCCCTGGAAGCCCAACTGCCATCTTCTTGACCCGGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1098

Amino Acids

124.09

Weight (kDa)

5.89

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 24 - 105 1e-09 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 194 - 754 2.8e-15 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 659 - 761 3.6e-08 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 668 - 744 1.7e-08 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 794 - 919 3.3e-16 Anticodon-binding domain of tRNA ligase
RBD_LARS1 PF24810 948 - 1024 2.9e-06 Leucine--tRNA ligase, RagD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2565
Acc36I ACCTGC 1 cut(s) 1249
Acc65I GGTACC 1 cut(s) 3188
AccB1I GGYRCC 3 cut(s) 248, 829, 3188
AccB7I CCANNNNNTGG 2 cut(s) 743, 3257
AccI GTMKAC 1 cut(s) 477
AccII CGCG 1 cut(s) 2218
AciI CCGC 8 cut(s) 5, 347, 1337, 1640, 2337, 2594, 2867, 3248
AclWI GGATC 2 cut(s) 533, 2040
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 6 cut(s) 530, 1319, 1454, 1663, 2198, 2260
AcuI CTGAAG 4 cut(s) 1452, 2468, 2742, 3080
AfaI GTAC 4 cut(s) 1168, 1604, 2504, 3190
AfiI CCNNNNNNNGG 7 cut(s) 131, 132, 571, 666, 743, 1322, 3257
AflIII ACRYGT 3 cut(s) 1854, 2445, 2570
AhlI ACTAGT 1 cut(s) 1181
AjiI CACGTC 1 cut(s) 2573
AjnI CCWGG 7 cut(s) 32, 130, 1473, 1763, 2184, 3196, 3256
AloI GAACNNNNNNTCC 4 cut(s) 1760, 1792, 2432, 2464
Alw21I GWGCWC 3 cut(s) 418, 1068, 3205
Alw26I GTCTC 7 cut(s) 33, 610, 896, 1673, 1845, 2267, 3115
AlwI GGATC 2 cut(s) 533, 2040
Ama87I CYCGRG 1 cut(s) 50
AoxI GGCC 8 cut(s) 6, 505, 1087, 1215, 1930, 2099, 2660, 3047
ApaI GGGCCC 1 cut(s) 3051
ApeKI GCWGC 9 cut(s) 224, 227, 452, 748, 881, 884, 1001, 1334, 2285
ApoI RAATTY 6 cut(s) 530, 1319, 1454, 1663, 2198, 2260
AseI ATTAAT 1 cut(s) 3233
Asp700I GAANNNNTTC 3 cut(s) 786, 872, 1424
Asp718I GGTACC 1 cut(s) 3188
AspLEI GCGC 1 cut(s) 2566
AspS9I GGNCC 7 cut(s) 1862, 1931, 2175, 2360, 3047, 3048, 3091
AsuC2I CCSGG 2 cut(s) 2438, 3286
AsuHPI GGTGA 4 cut(s) 1095, 1463, 1492, 1580
AsuII TTCGAA 3 cut(s) 70, 960, 1458
AsuNHI GCTAGC 1 cut(s) 1390
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 4 cut(s) 1862, 2175, 2360, 3091
BaeGI GKGCMC 2 cut(s) 2127, 3051
BanI GGYRCC 3 cut(s) 248, 829, 3188
BanII GRGCYC 2 cut(s) 1068, 3051
BauI CACGAG 1 cut(s) 2103
BbsI GAAGAC 1 cut(s) 588
Bbv12I GWGCWC 3 cut(s) 418, 1068, 3205
BbvI GCAGC 9 cut(s) 211, 239, 439, 760, 868, 896, 1013, 1321, 2272
BceAI ACGGC 1 cut(s) 2091
BcgI CGANNNNNNTGC 4 cut(s) 2084, 2118, 3182, 3216
BciT130I CCWGG 7 cut(s) 34, 132, 1475, 1765, 2186, 3198, 3258
BciVI GTATCC 2 cut(s) 2013, 2874
BcnI CCSGG 2 cut(s) 2438, 3286
BcoDI GTCTC 7 cut(s) 33, 610, 896, 1673, 1845, 2267, 3115
BcuI ACTAGT 1 cut(s) 1181
BfaI CTAG 5 cut(s) 215, 1032, 1182, 1391, 2493
BfmI CTRYAG 3 cut(s) 225, 882, 2885
BfuAI ACCTGC 1 cut(s) 1249
BfuI GTATCC 2 cut(s) 2013, 2874
BglII AGATCT 2 cut(s) 1354, 2680
Bme1390I CCNGG 9 cut(s) 34, 132, 1475, 1765, 2186, 2438, 3198, 3258, 3286
Bme18I GGWCC 4 cut(s) 1862, 2175, 2360, 3091
BmeT110I CYCGRG 1 cut(s) 50
BmgBI CACGTC 1 cut(s) 2573
BmgT120I GGNCC 7 cut(s) 1862, 1931, 2175, 2360, 3047, 3048, 3091
BmiI GGNNCC 9 cut(s) 250, 831, 955, 1038, 1620, 1864, 3049, 3050, 3190
BmrFI CCNGG 9 cut(s) 34, 132, 1475, 1765, 2186, 2438, 3198, 3258, 3286
BmrI ACTGGG 2 cut(s) 1179, 2478
BmtI GCTAGC 1 cut(s) 1394
BmuI ACTGGG 2 cut(s) 1179, 2478
BpiI GAAGAC 1 cut(s) 588
BplI GAGNNNNNCTC 2 cut(s) 2445, 2477
BpmI CTGGAG 6 cut(s) 634, 2259, 2635, 2661, 2907, 2960
Bpu14I TTCGAA 3 cut(s) 70, 960, 1458
BpuEI CTTGAG 3 cut(s) 572, 2447, 3034
BpuMI CCSGG 2 cut(s) 2438, 3286
BsaI GGTCTC 2 cut(s) 33, 896
BsaJI CCNNGG 5 cut(s) 131, 1763, 1764, 3196, 3256
BsaXI ACNNNNNCTCC 8 cut(s) 1637, 1667, 1979, 2009, 2232, 2262, 2905, 2935
Bsc4I CCNNNNNNNGG 7 cut(s) 131, 132, 571, 666, 743, 1322, 3257
Bse118I RCCGGY 1 cut(s) 349
Bse1I ACTGG 8 cut(s) 289, 617, 744, 1174, 1641, 2102, 2473, 2792
Bse3DI GCAATG 1 cut(s) 2125
BseBI CCWGG 7 cut(s) 34, 132, 1475, 1765, 2186, 3198, 3258
BseDI CCNNGG 5 cut(s) 131, 1763, 1764, 3196, 3256
BseLI CCNNNNNNNGG 7 cut(s) 131, 132, 571, 666, 743, 1322, 3257
BseMI GCAATG 1 cut(s) 2125
BseMII CTCAG 5 cut(s) 525, 891, 1533, 2133, 2415
BseNI ACTGG 8 cut(s) 289, 617, 744, 1174, 1641, 2102, 2473, 2792
BseRI GAGGAG 1 cut(s) 1658
BseSI GKGCMC 2 cut(s) 2127, 3051
BseX3I CGGCCG 1 cut(s) 6
BseXI GCAGC 9 cut(s) 211, 239, 439, 760, 868, 896, 1013, 1321, 2272
BseYI CCCAGC 1 cut(s) 1865
BsgI GTGCAG 3 cut(s) 265, 777, 1371
Bsh1236I CGCG 1 cut(s) 2218
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 8 cut(s) 8, 507, 1089, 1217, 1932, 2101, 2662, 3049
BshNI GGYRCC 3 cut(s) 248, 829, 3188
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 3 cut(s) 418, 1068, 3205
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 3 cut(s) 350, 2437, 3286
BslFI GGGAC 4 cut(s) 1765, 1848, 3159, 3165
BslI CCNNNNNNNGG 7 cut(s) 131, 132, 571, 666, 743, 1322, 3257
BsmAI GTCTC 7 cut(s) 33, 610, 896, 1673, 1845, 2267, 3115
BsmBI CGTCTC 1 cut(s) 610
BsmFI GGGAC 4 cut(s) 1765, 1848, 3159, 3165
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 8 cut(s) 8, 507, 1089, 1217, 1932, 2101, 2662, 3049
Bso31I GGTCTC 2 cut(s) 33, 896
BsoBI CYCGRG 1 cut(s) 50
Bsp119I TTCGAA 3 cut(s) 70, 960, 1458
Bsp120I GGGCCC 1 cut(s) 3047
Bsp1286I GDGCHC 5 cut(s) 418, 1068, 2127, 3051, 3205
BspACI CCGC 8 cut(s) 5, 347, 1337, 1640, 2337, 2594, 2867, 3248
BspANI GGCC 8 cut(s) 8, 507, 1089, 1217, 1932, 2101, 2662, 3049
BspCNI CTCAG 5 cut(s) 524, 892, 1534, 2134, 2416
BspFNI CGCG 1 cut(s) 2218
BspHI TCATGA 3 cut(s) 763, 1075, 1672
BspLI GGNNCC 9 cut(s) 250, 831, 955, 1038, 1620, 1864, 3049, 3050, 3190
BspMAI CTGCAG 2 cut(s) 229, 886
BspMI ACCTGC 1 cut(s) 1249
BspOI GCTAGC 1 cut(s) 1394
BspPI GGATC 2 cut(s) 533, 2040
BspQI GCTCTTC 1 cut(s) 17
BspT104I TTCGAA 3 cut(s) 70, 960, 1458
BspT107I GGYRCC 3 cut(s) 248, 829, 3188
BspTNI GGTCTC 2 cut(s) 33, 896
BsrDI GCAATG 1 cut(s) 2125
BsrFI RCCGGY 1 cut(s) 349
BsrI ACTGG 8 cut(s) 289, 617, 744, 1174, 1641, 2102, 2473, 2792
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 5 cut(s) 131, 1763, 1764, 3196, 3256
BssNAI GTATAC 1 cut(s) 478
BssSI CACGAG 1 cut(s) 2103
Bst1107I GTATAC 1 cut(s) 478
Bst2BI CACGAG 1 cut(s) 2103
Bst2UI CCWGG 7 cut(s) 34, 132, 1475, 1765, 2186, 3198, 3258
Bst4CI ACNGT 5 cut(s) 621, 1150, 1488, 2537, 2903
Bst6I CTCTTC 5 cut(s) 17, 78, 1140, 1541, 1767
BstAPI GCANNNNNTGC 4 cut(s) 401, 1241, 2131, 2285
BstBI TTCGAA 3 cut(s) 70, 960, 1458
BstC8I GCNNGC 4 cut(s) 290, 1191, 1392, 1502
BstENI CCTNNNNNAGG 1 cut(s) 664
BstFNI CGCG 1 cut(s) 2218
BstHHI GCGC 1 cut(s) 2566
BstMAI GTCTC 7 cut(s) 33, 610, 896, 1673, 1845, 2267, 3115
BstMCI CGRYCG 1 cut(s) 9
BstNI CCWGG 7 cut(s) 34, 132, 1475, 1765, 2186, 3198, 3258
BstNSI RCATGY 4 cut(s) 197, 292, 1858, 2449
BstSCI CCNGG 9 cut(s) 32, 130, 1473, 1763, 2184, 2436, 3196, 3256, 3284
BstSFI CTRYAG 3 cut(s) 225, 882, 2885
BstSLI GKGCMC 2 cut(s) 2127, 3051
BstUI CGCG 1 cut(s) 2218
BstV1I GCAGC 9 cut(s) 211, 239, 439, 760, 868, 896, 1013, 1321, 2272
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 4 cut(s) 538, 1354, 2032, 2680
BstXI CCANNNNNNTGG 1 cut(s) 549
BstYI RGATCY 4 cut(s) 538, 1354, 2032, 2680
BstZ17I GTATAC 1 cut(s) 478
BstZI CGGCCG 1 cut(s) 6
BsuI GTATCC 2 cut(s) 2013, 2874
BsuRI GGCC 8 cut(s) 8, 507, 1089, 1217, 1932, 2101, 2662, 3049
BtrI CACGTC 1 cut(s) 2573
BtsI GCAGTG 2 cut(s) 277, 582
BtsIMutI CAGTG 8 cut(s) 277, 282, 582, 737, 1428, 1727, 2095, 2908
BveI ACCTGC 1 cut(s) 1249
Cac8I GCNNGC 4 cut(s) 290, 1191, 1392, 1502
CciI TCATGA 3 cut(s) 763, 1075, 1672
CfoI GCGC 1 cut(s) 2566
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 7 cut(s) 1862, 1931, 2175, 2360, 3047, 3048, 3091
CseI GACGC 1 cut(s) 291
Csp6I GTAC 4 cut(s) 1167, 1603, 2503, 3189
CviQI GTAC 4 cut(s) 1167, 1603, 2503, 3189
DraI TTTAAA 1 cut(s) 1230
EaeI YGGCCR 1 cut(s) 6
EagI CGGCCG 1 cut(s) 6
Eam1104I CTCTTC 5 cut(s) 17, 78, 1140, 1541, 1767
EarI CTCTTC 5 cut(s) 17, 78, 1140, 1541, 1767
EciI GGCGGA 3 cut(s) 1655, 2882, 3237
Ecl136II GAGCTC 1 cut(s) 1066
EclXI CGGCCG 1 cut(s) 6
Eco24I GRGCYC 2 cut(s) 1068, 3051
Eco31I GGTCTC 2 cut(s) 33, 896
Eco32I GATATC 1 cut(s) 525
Eco47I GGWCC 4 cut(s) 1862, 2175, 2360, 3091
Eco52I CGGCCG 1 cut(s) 6
Eco53kI GAGCTC 1 cut(s) 1066
Eco57I CTGAAG 4 cut(s) 1452, 2468, 2742, 3080
Eco88I CYCGRG 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 1066
EcoNI CCTNNNNNAGG 1 cut(s) 664
EcoO109I RGGNCCY 3 cut(s) 2360, 3048, 3091
EcoRI GAATTC 1 cut(s) 1454
EcoRII CCWGG 7 cut(s) 32, 130, 1473, 1763, 2184, 3196, 3256
EcoRV GATATC 1 cut(s) 525
EcoT22I ATGCAT 2 cut(s) 199, 928
EcoT38I GRGCYC 2 cut(s) 1068, 3051
Esp3I CGTCTC 1 cut(s) 610
FalI AAGNNNNNCTT 4 cut(s) 861, 893, 1425, 1457
FaqI GGGAC 4 cut(s) 1765, 1848, 3159, 3165
FauI CCCGC 1 cut(s) 354
FauNDI CATATG 2 cut(s) 1612, 2374
FblI GTMKAC 1 cut(s) 477
FriOI GRGCYC 2 cut(s) 1068, 3051
FspBI CTAG 5 cut(s) 215, 1032, 1182, 1391, 2493
FspI TGCGCA 1 cut(s) 2565
GlaI GCGC 1 cut(s) 2565
GsaI CCCAGC 1 cut(s) 1869
GsuI CTGGAG 6 cut(s) 634, 2259, 2635, 2661, 2907, 2960
HaeIII GGCC 8 cut(s) 8, 507, 1089, 1217, 1932, 2101, 2662, 3049
HapII CCGG 3 cut(s) 350, 2437, 3286
HgaI GACGC 1 cut(s) 291
HhaI GCGC 1 cut(s) 2566
Hin6I GCGC 1 cut(s) 2564
HinP1I GCGC 1 cut(s) 2564
HincII GTYRAC 2 cut(s) 555, 1146
HindII GTYRAC 2 cut(s) 555, 1146
HindIII AAGCTT 2 cut(s) 2738, 2996
HpaII CCGG 3 cut(s) 350, 2437, 3286
HphI GGTGA 4 cut(s) 1095, 1463, 1492, 1580
Hpy188I TCNGA 9 cut(s) 514, 1023, 1122, 1798, 2453, 2671, 2685, 2970, 3080
HpyCH4III ACNGT 5 cut(s) 621, 1150, 1488, 2537, 2903
HpyCH4IV ACGT 1 cut(s) 2572
HpySE526I ACGT 1 cut(s) 2572
HspAI GCGC 1 cut(s) 2564
KpnI GGTACC 1 cut(s) 3192
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 5 cut(s) 413, 953, 1063, 1497, 2926
Lsp1109I GCAGC 9 cut(s) 211, 239, 439, 760, 868, 896, 1013, 1321, 2272
MaeI CTAG 5 cut(s) 215, 1032, 1182, 1391, 2493
MaeII ACGT 1 cut(s) 2572
MaeIII GTNAC 8 cut(s) 113, 608, 988, 1101, 1177, 1557, 2891, 3037
MfeI CAATTG 4 cut(s) 547, 1524, 2190, 2961
MflI RGATCY 4 cut(s) 538, 1354, 2032, 2680
MhlI GDGCHC 5 cut(s) 418, 1068, 2127, 3051, 3205
MlyI GAGTC 4 cut(s) 388, 1520, 1747, 1796
MmeI TCCRAC 1 cut(s) 837
Mph1103I ATGCAT 2 cut(s) 199, 928
MroXI GAANNNNTTC 3 cut(s) 786, 872, 1424
MslI CAYNNNNRTG 2 cut(s) 287, 815
MspI CCGG 3 cut(s) 350, 2437, 3286
MspR9I CCNGG 9 cut(s) 34, 132, 1475, 1765, 2186, 2438, 3198, 3258, 3286
MunI CAATTG 4 cut(s) 547, 1524, 2190, 2961
Mva1269I GAATGC 1 cut(s) 197
MvaI CCWGG 7 cut(s) 34, 132, 1475, 1765, 2186, 3198, 3258
MvnI CGCG 1 cut(s) 2218
NciI CCSGG 2 cut(s) 2438, 3286
NdeI CATATG 2 cut(s) 1612, 2374
NheI GCTAGC 1 cut(s) 1390
NlaIV GGNNCC 9 cut(s) 250, 831, 955, 1038, 1620, 1864, 3049, 3050, 3190
NmuCI GTSAC 5 cut(s) 113, 608, 1101, 1177, 1557
NsbI TGCGCA 1 cut(s) 2565
NsiI ATGCAT 2 cut(s) 199, 928
NspI RCATGY 4 cut(s) 197, 292, 1858, 2449
NspV TTCGAA 3 cut(s) 70, 960, 1458
PacI TTAATTAA 1 cut(s) 3117
PaeI GCATGC 1 cut(s) 292
PaeR7I CTCGAG 1 cut(s) 50
PagI TCATGA 3 cut(s) 763, 1075, 1672
PasI CCCWGGG 1 cut(s) 1764
PciI ACATGT 2 cut(s) 1854, 2445
PciSI GCTCTTC 1 cut(s) 17
PcsI WCGNNNNNNNCGW 1 cut(s) 966
PctI GAATGC 1 cut(s) 197
PdmI GAANNNNTTC 3 cut(s) 786, 872, 1424
PflFI GACNNNGTC 1 cut(s) 3286
PflMI CCANNNNNTGG 2 cut(s) 743, 3257
PfoI TCCNGGA 1 cut(s) 1473
PleI GAGTC 4 cut(s) 387, 1519, 1747, 1795
PpsI GAGTC 4 cut(s) 387, 1519, 1747, 1795
PpuMI RGGWCCY 2 cut(s) 2360, 3091
PscI ACATGT 2 cut(s) 1854, 2445
PshBI ATTAAT 1 cut(s) 3233
Psp124BI GAGCTC 1 cut(s) 1068
Psp5II RGGWCCY 2 cut(s) 2360, 3091
Psp6I CCWGG 7 cut(s) 32, 130, 1473, 1763, 2184, 3196, 3256
PspFI CCCAGC 1 cut(s) 1865
PspGI CCWGG 7 cut(s) 32, 130, 1473, 1763, 2184, 3196, 3256
PspN4I GGNNCC 9 cut(s) 250, 831, 955, 1038, 1620, 1864, 3049, 3050, 3190
PspOMI GGGCCC 1 cut(s) 3047
PspPI GGNCC 7 cut(s) 1862, 1931, 2175, 2360, 3047, 3048, 3091
PspPPI RGGWCCY 2 cut(s) 2360, 3091
PstI CTGCAG 2 cut(s) 229, 886
PsuI RGATCY 4 cut(s) 538, 1354, 2032, 2680
PsyI GACNNNGTC 1 cut(s) 3286
RsaI GTAC 4 cut(s) 1168, 1604, 2504, 3190
RsaNI GTAC 4 cut(s) 1167, 1603, 2503, 3189
RseI CAYNNNNRTG 2 cut(s) 287, 815
SacI GAGCTC 1 cut(s) 1068
SapI GCTCTTC 1 cut(s) 17
Sau96I GGNCC 7 cut(s) 1862, 1931, 2175, 2360, 3047, 3048, 3091
SchI GAGTC 4 cut(s) 388, 1520, 1747, 1796
ScrFI CCNGG 9 cut(s) 34, 132, 1475, 1765, 2186, 2438, 3198, 3258, 3286
SduI GDGCHC 5 cut(s) 418, 1068, 2127, 3051, 3205
SfcI CTRYAG 3 cut(s) 225, 882, 2885
Sfr274I CTCGAG 1 cut(s) 50
SfuI TTCGAA 3 cut(s) 70, 960, 1458
SinI GGWCC 4 cut(s) 1862, 2175, 2360, 3091
SlaI CTCGAG 1 cut(s) 50
SmiMI CAYNNNNRTG 2 cut(s) 287, 815
SmlI CTYRAG 4 cut(s) 50, 587, 2462, 3013
SmoI CTYRAG 4 cut(s) 50, 587, 2462, 3013
SpeI ACTAGT 1 cut(s) 1181
SphI GCATGC 1 cut(s) 292
SsiI CCGC 8 cut(s) 5, 347, 1337, 1640, 2337, 2594, 2867, 3248
SspI AATATT 1 cut(s) 2407
SspMI CTAG 5 cut(s) 215, 1032, 1182, 1391, 2493
SstI GAGCTC 1 cut(s) 1068
StyD4I CCNGG 9 cut(s) 32, 130, 1473, 1763, 2184, 2436, 3196, 3256, 3284
TaaI ACNGT 5 cut(s) 621, 1150, 1488, 2537, 2903
TaiI ACGT 1 cut(s) 2575
TaqI TCGA 7 cut(s) 51, 70, 960, 1458, 2017, 2804, 3159
TatI WGTACW 1 cut(s) 2502
TauI GCSGC 2 cut(s) 8, 2339
TscAI CASTG 8 cut(s) 284, 289, 582, 744, 1435, 1727, 2102, 2908
TseFI GTSAC 5 cut(s) 113, 608, 1101, 1177, 1557
TseI GCWGC 9 cut(s) 224, 227, 452, 748, 881, 884, 1001, 1334, 2285
Tsp45I GTSAC 5 cut(s) 113, 608, 1101, 1177, 1557
TspDTI ATGAA 7 cut(s) 185, 656, 1676, 1785, 1994, 2502, 2546
TspGWI ACGGA 1 cut(s) 707
TspRI CASTG 8 cut(s) 284, 289, 582, 744, 1435, 1727, 2102, 2908
Tth111I GACNNNGTC 1 cut(s) 3286
Van91I CCANNNNNTGG 2 cut(s) 743, 3257
VpaK11BI GGWCC 4 cut(s) 1862, 2175, 2360, 3091
VspI ATTAAT 1 cut(s) 3233
XagI CCTNNNNNAGG 1 cut(s) 664
XapI RAATTY 6 cut(s) 530, 1319, 1454, 1663, 2198, 2260
XceI RCATGY 4 cut(s) 197, 292, 1858, 2449
XhoI CTCGAG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 477
XmnI GAANNNNTTC 3 cut(s) 786, 872, 1424
XspI CTAG 5 cut(s) 215, 1032, 1182, 1391, 2493
Zsp2I ATGCAT 2 cut(s) 199, 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.