Rw1G021850

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000598
Physical Location & Seq
Reverse (-)
46348718 .. 46352330
3613 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G021850.1

Sequence Viewer

Length: 3126 bp
ATGAAGATGAAGTCAATAAGGAGAGACCGTCTTCGAAAGATTGAGGAAGACACTCAAAGACGGTGGGAAGAGAAGCAAGTTTTCAGGGCTGAACCCCGTGAAAATCCTCCCCAACCAGGAGAAAAGTTTTTCGGAAATTTCCCTTTCCCGTACATGAATGGTTTCCTTCATCTGGGACATGCATTCACCATCTCCAAGCTTGAGTTTGCTGCCGCTTATCATAGGCTAAGAGGTGTCAATGTGCTCTTACCATTTGCTTTCCATTGCACCGGAATGCCCATCAAAGCTGCCGCCGATAAACTTGCAAGAGAGCTCCAACAGTTTGGCTACCCTCCAGTATTTCCCATTGATGTGAAGCAAGATGAAATTGCAGCCAAAGCAAAAGCCGAAGCTGGAGCAGAGAAAAAACATAAGGGAAAGAAGGCCAAGGCTGCAGCAAAGTTCGCCGGACAAGTCTTCCAATGGGAAATTATGCGTGATGGTTTCGGATTGTCTGACAGTGAGATATCCGAGTTCCAGGACCCGGAAAAGTGGGTTAAGTTTTTCCCACCACGTGGGGTGGAAGACCTGAAAGCTTTTGGGTTGGGATGTGATTGGAGACGTTCCTTTATTACTACAGACATGAACCCTTTCTTTGACAACTTTGTGAAATGGCAGGTTAGGAAGCTCAAATCCAAGGGTAAGATTGTGAAAGATGTCCGGTACACTATATTCTCCCCATTCGATGGGCAGCCGTGTGCAGATCATGATAGGGCAAGAGGAGAAGGAGTTCAACCACAAGAATACATTGTCATCAAAATGGAGGTAGTAGCACCTTTACCCTCAAAATTTGGATCAGTGTTGAAAGGGAGGAAGGTATTCTTAGCAGCAGCAACGTTGAGACCCGAGACCATGTATGGACAAACAAATGCATGGGTATTACCCGATGCTAAGTATGGAGCCTTTGAGATCAACGATACGGATGTCTTTATTCTTACACATAGAGCAGCACTTAATTTTGCTTATCAGAAGTACTCTAGGGTCCCAGAGAAACCTACTTGCTTGGTCGAACTGACTGGTTCTGATTTGATTGGCCTTCCATTGAAGTCACCACTTGCATTCAGTCATATCATTTATGCCCTTCCTATGTTGACCATCCCAACTGACAAAGGCACTGGGATAGTGACCAGCGTACCTAGTGATGCTCCTGATGATTATATGGCTCTGCATAATTTAAAGTCAAAATCTGCTTTGAGGGAAAAATATGGTGTGAAGGAAGAATGGGTGATGCCGTTTGAGGTTATCCCAATCATCAAAGAAGGAACACTGACCGTGGGAGAATTCATAGGAAGGGAAGTGCAGGAGGCGAAACCCTTAATAAAAAGGAAGCTGATAGAGGCGGGAGAAGGAATTATATATTATGAACCTGAGGAGCAAGTTGTGTCCCGGTCGGGTGATGAATGTGTTGTGGCACTTACAGATCAGTGGTACATCACATATGGGGAAACTGAATGGAAAAAGATGGCTGAAGAGTGCTTGTCCGGCATGAGCTTATATTCCGATGAGACTCGGCATGGATTTGAGCACACTATAGGGTGGTTGAATCAGTGGGCTTGTTCACGATCTTTTGGACTTGGGACTCGTATCCCCTGGGATGAAGAATTCTTAGTTGAGTCACTGTCTGATTCAACTATTTACATGGCTTATTACACCATTGCTCATTTCTTACACAATGGAGATATGTATGGTGCCAGCAGGTCAGAAGTTAAACCTGAGCAAATGACTGATGACGTCTGGGATTTTATTTTCTGTGATGGCCCATATCCCAAGTCTTCTGACATCTCATCATCAATTCTTAAGAAAATGAAGAAAGAGTTTGAATATTGGTATCCTTTTGATCTACGAGTGTCAGGCAAGGATTTGATCCAAAACCATCTAGCCTTCTGCATATACAACCACACGGCTATTATGACGTCCAACAAGTACTGGCCTCGTGGGTTCAGATGTAATGGGCACCTCTTGCTAAATGGTGAGAAGATGTCCAAGTCTACAGTGAACTTTAGGACCCTTCGCCAGGCAATTGAGGAATTTTCTGCTGATGCCACTCGGTTCTCTTTGGCTGATGCCGGAGATGGTGTGGATGATGCAAATTTTGTATTTGTGACTGCCAATGCTGCAATCTTGCGGCTCACTAAAGAGATTGCATGGATTGAACAAGTTTTGGCTTCAGACTCTTGTTTGAGAACAGGTCCCCCATCTACTTATGCTGACAGGGTCTTTGCTAATGAGATAAACATTGCTGTACACAGGACTGAGCTGAATTACGGAGATTACATGTTCCGAGAAGCCCTCAAGACTGGGTTTTATGACCTTCAAGCAGCCAGGGATGAGTACAAGTTTTCGTGCGGTGCTGGGGGCATGAACCGTGATTTGGTATGGCGTTTTGTGGATGTGCAGACGCGTCTTATTACTCCAATCTGCCCACACTATGCAGAATATGTGTGGAGGGAACTTTTGAACAAGGAAGGGTTTGTGGTAAATGCAGGATGGCCTGTTGCTGATGCTTCAGACTTAACCCTCCAGAGTGCGAATAAGTATTTGCAAGACTTCATTGTTTTAATGAGGAAGCTTTTTCACAAGCAAGTTTCAGGTTCAAATAAAGGCCATAAGGGTACTTCAGTTACATTGATCACTGGAGAGAACAAGCTAACTGGCTTGGTATATGTTAATGAGCATTTTGATGGGTGGAAGGCAGAGTGCTTGAAAATACTTCAAATTCACTTTAACAGTGACACTCGTACATTTTCTTCTGACAAGCTGATACAGGAGGCGCTACAAAAAAGTTCTATTGGTCACTCTAAAGATTTTCGAGAAACCCTGAAGTTGTGTATGCCTTTCATAAGGTCAAAGATGGATGAAGCAATTGCGCTTGGGGCTCGTGCCTTGGACTTAATGTTGCCATTTGGAGAGATTGAGGTCCTTCATGAGAACTTGGACTTGATCCAGAGGCAAATCGGTCTTCAAGAGTTGCATGTTTTGTCCTTTGCCAACCCTGATGCTCGGGCTTTAGCTGGTCCTCATGTCAAGCAGATAGATCGGAATCCTCCATTTCCTGGGAGCCCCACTGCCATCTTCTTGACTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1041

Amino Acids

118.33

Weight (kDa)

6.63

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 19 - 100 5.3e-08 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 182 - 710 3.6e-16 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 615 - 714 5.8e-08 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 623 - 700 3.1e-06 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 750 - 870 9.5e-13 Anticodon-binding domain of tRNA ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1773, 1955
Acc36I ACCTGC 2 cut(s) 646, 1725
AccB1I GGYRCC 2 cut(s) 1727, 1992
AccB7I CCANNNNNTGG 3 cut(s) 554, 725, 3092
AccI GTMKAC 1 cut(s) 2027
AccII CGCG 1 cut(s) 2440
AciI CCGC 5 cut(s) 213, 291, 1379, 2164, 2385
AclI AACGTT 1 cut(s) 875
AclWI GGATC 3 cut(s) 841, 1897, 2974
AcsI RAATTY 7 cut(s) 136, 827, 1319, 1640, 2066, 2128, 2754
AcuI CTGAAG 5 cut(s) 1527, 2190, 2529, 2640, 2879
AcvI CACGTG 1 cut(s) 554
AcyI GRCGYC 2 cut(s) 1770, 1952
AdeI CACNNNGTG 1 cut(s) 554
AfiI CCNNNNNNNGG 8 cut(s) 116, 172, 523, 554, 725, 2053, 2410, 3092
AflII CTTAAG 1 cut(s) 1835
AflIII ACRYGT 2 cut(s) 2313, 2438
AjnI CCWGG 6 cut(s) 115, 516, 1628, 2052, 2360, 3091
AjuI GAANNNNNNNTTGG 2 cut(s) 2393, 2425
AloI GAACNNNNNNTCC 2 cut(s) 753, 785
Alw21I GWGCWC 3 cut(s) 246, 315, 1566
Alw26I GTCTC 5 cut(s) 18, 592, 874, 881, 1538
AlwI GGATC 3 cut(s) 841, 1897, 2974
Ama87I CYCGRG 2 cut(s) 884, 3039
AoxI GGCC 6 cut(s) 423, 1072, 1795, 1967, 2528, 2641
ApoI RAATTY 7 cut(s) 136, 827, 1319, 1640, 2066, 2128, 2754
ArsI GACNNNNNNTTYG 2 cut(s) 2240, 2272
Asp700I GAANNNNTTC 4 cut(s) 161, 629, 768, 857
AspLEI GCGC 2 cut(s) 2812, 2908
AspS9I GGNCC 7 cut(s) 520, 1021, 1796, 2043, 2228, 2956, 3053
AsuC2I CCSGG 2 cut(s) 524, 1426
AsuHPI GGTGA 5 cut(s) 178, 1080, 1276, 1445, 2021
AsuII TTCGAA 1 cut(s) 34
AvaI CYCGRG 2 cut(s) 884, 3039
AvaII GGWCC 6 cut(s) 520, 1021, 2043, 2228, 2956, 3053
AxyI CCTNAGG 1 cut(s) 1407
BaeGI GKGCMC 1 cut(s) 1995
BaeI ACNNNNGTAYC 6 cut(s) 948, 981, 1155, 1188, 2643, 2676
BanI GGYRCC 2 cut(s) 1727, 1992
BanII GRGCYC 3 cut(s) 315, 2917, 3101
BarI GAAGNNNNNNTAC 2 cut(s) 2770, 2802
BauI CACGAG 2 cut(s) 1971, 2916
BbrPI CACGTG 1 cut(s) 554
BbsI GAAGAC 6 cut(s) 23, 54, 448, 570, 1803, 2990
Bbv12I GWGCWC 3 cut(s) 246, 315, 1566
BceAI ACGGC 3 cut(s) 718, 1255, 1956
BcgI CGANNNNNNTGC 4 cut(s) 284, 318, 3056, 3090
BciT130I CCWGG 6 cut(s) 117, 518, 1630, 2054, 2362, 3093
BciVI GTATCC 2 cut(s) 1634, 1878
BclI TGATCA 1 cut(s) 2667
BcnI CCSGG 2 cut(s) 524, 1426
BcoDI GTCTC 5 cut(s) 18, 592, 874, 881, 1538
BfaI CTAG 3 cut(s) 1017, 1176, 1916
BfmI CTRYAG 4 cut(s) 432, 615, 1569, 2028
BfoI RGCGCY 1 cut(s) 2813
BfrI CTTAAG 1 cut(s) 1835
BfuAI ACCTGC 2 cut(s) 646, 1725
BfuI GTATCC 2 cut(s) 1634, 1878
BmcAI AGTACT 2 cut(s) 1013, 1964
Bme1390I CCNGG 8 cut(s) 117, 518, 524, 1426, 1630, 2054, 2362, 3093
Bme18I GGWCC 6 cut(s) 520, 1021, 2043, 2228, 2956, 3053
BmeT110I CYCGRG 2 cut(s) 884, 3039
BmgT120I GGNCC 7 cut(s) 520, 1021, 1796, 2043, 2228, 2956, 3053
BmiI GGNNCC 9 cut(s) 522, 940, 1022, 1023, 1729, 1994, 2045, 2230, 3098
BmrFI CCNGG 8 cut(s) 117, 518, 524, 1426, 1630, 2054, 2362, 3093
BmrI ACTGGG 2 cut(s) 1164, 2346
BmsI GCATC 8 cut(s) 916, 1171, 1257, 2068, 2092, 2113, 2530, 3025
BmuI ACTGGG 2 cut(s) 1164, 2346
BpiI GAAGAC 6 cut(s) 23, 54, 448, 570, 1803, 2990
BplI GAGNNNNNCTC 2 cut(s) 2313, 2345
BpmI CTGGAG 4 cut(s) 318, 414, 2543, 2694
Bpu10I CCTNAGC 1 cut(s) 1752
Bpu14I TTCGAA 1 cut(s) 34
BpuEI CTTGAG 2 cut(s) 221, 2315
BpuMI CCSGG 2 cut(s) 524, 1426
BsaAI YACGTR 1 cut(s) 554
BsaBI GATNNNNATC 1 cut(s) 3078
BsaHI GRCGYC 2 cut(s) 1770, 1952
BsaI GGTCTC 3 cut(s) 18, 874, 881
BsaJI CCNNGG 8 cut(s) 426, 675, 1311, 1628, 1629, 2361, 2922, 3092
BsaWI WCCGGW 2 cut(s) 269, 699
BsaXI ACNNNNNCTCC 6 cut(s) 13, 43, 753, 783, 2100, 2130
Bsc4I CCNNNNNNNGG 8 cut(s) 116, 172, 523, 554, 725, 2053, 2410, 3092
Bse1I ACTGG 7 cut(s) 335, 1060, 1159, 1970, 2341, 2677, 2695
Bse21I CCTNAGG 1 cut(s) 1407
Bse3DI GCAATG 3 cut(s) 262, 1692, 2274
Bse8I GATNNNNATC 1 cut(s) 3078
BseBI CCWGG 6 cut(s) 117, 518, 1630, 2054, 2362, 3093
BseDI CCNNGG 8 cut(s) 426, 675, 1311, 1628, 1629, 2361, 2922, 3092
BseGI GGATG 9 cut(s) 593, 967, 1134, 1639, 2125, 2371, 2434, 2531, 2899
BseJI GATNNNNATC 1 cut(s) 3078
BseLI CCNNNNNNNGG 8 cut(s) 116, 172, 523, 554, 725, 2053, 2410, 3092
BseMI GCAATG 3 cut(s) 262, 1692, 2274
BseMII CTCAG 3 cut(s) 1398, 1743, 2283
BseNI ACTGG 7 cut(s) 335, 1060, 1159, 1970, 2341, 2677, 2695
BseRI GAGGAG 2 cut(s) 774, 1424
BseSI GKGCMC 1 cut(s) 1995
BseYI CCCAGC 1 cut(s) 2390
BsgI GTGCAG 3 cut(s) 759, 1358, 2453
Bsh1236I CGCG 1 cut(s) 2440
Bsh1285I CGRYCG 1 cut(s) 1430
BshFI GGCC 6 cut(s) 425, 1074, 1797, 1969, 2530, 2643
BshNI GGYRCC 2 cut(s) 1727, 1992
BsiEI CGRYCG 1 cut(s) 1430
BsiHKAI GWGCWC 3 cut(s) 246, 315, 1566
BsiHKCI CYCGRG 2 cut(s) 884, 3039
BsiSI CCGG 7 cut(s) 270, 447, 524, 700, 1426, 1521, 2106
BslFI GGGAC 5 cut(s) 189, 1007, 1408, 1630, 2214
BslI CCNNNNNNNGG 8 cut(s) 116, 172, 523, 554, 725, 2053, 2410, 3092
BsmAI GTCTC 5 cut(s) 18, 592, 874, 881, 1538
BsmBI CGTCTC 1 cut(s) 592
BsmFI GGGAC 5 cut(s) 189, 1007, 1408, 1630, 2214
BsmI GAATGC 3 cut(s) 182, 279, 1097
BsnI GGCC 6 cut(s) 425, 1074, 1797, 1969, 2530, 2643
Bso31I GGTCTC 3 cut(s) 18, 874, 881
BsoBI CYCGRG 2 cut(s) 884, 3039
Bsp119I TTCGAA 1 cut(s) 34
Bsp1286I GDGCHC 6 cut(s) 246, 315, 1566, 1995, 2917, 3101
Bsp1407I TGTACA 1 cut(s) 2281
BspACI CCGC 5 cut(s) 213, 291, 1379, 2164, 2385
BspANI GGCC 6 cut(s) 425, 1074, 1797, 1969, 2530, 2643
BspCNI CTCAG 3 cut(s) 1399, 1744, 2284
BspFNI CGCG 1 cut(s) 2440
BspHI TCATGA 2 cut(s) 745, 2962
BspLI GGNNCC 9 cut(s) 522, 940, 1022, 1023, 1729, 1994, 2045, 2230, 3098
BspMAI CTGCAG 1 cut(s) 436
BspMI ACCTGC 2 cut(s) 646, 1725
BspPI GGATC 3 cut(s) 841, 1897, 2974
BspT104I TTCGAA 1 cut(s) 34
BspT107I GGYRCC 2 cut(s) 1727, 1992
BspTI CTTAAG 1 cut(s) 1835
BspTNI GGTCTC 3 cut(s) 18, 874, 881
BsrDI GCAATG 3 cut(s) 262, 1692, 2274
BsrGI TGTACA 1 cut(s) 2281
BsrI ACTGG 7 cut(s) 335, 1060, 1159, 1970, 2341, 2677, 2695
BssECI CCNNGG 8 cut(s) 426, 675, 1311, 1628, 1629, 2361, 2922, 3092
BssNI GRCGYC 2 cut(s) 1770, 1952
BssSI CACGAG 2 cut(s) 1971, 2916
BssT1I CCWWGG 3 cut(s) 426, 675, 2922
Bst2BI CACGAG 2 cut(s) 1971, 2916
Bst2UI CCWGG 6 cut(s) 117, 518, 1630, 2054, 2362, 3093
Bst4CI ACNGT 9 cut(s) 29, 63, 321, 500, 1312, 1659, 2032, 2405, 2768
Bst6I CTCTTC 2 cut(s) 63, 1503
BstACI GRCGYC 2 cut(s) 1770, 1952
BstAFI CTTAAG 1 cut(s) 1835
BstAPI GCANNNNNTGC 1 cut(s) 1999
BstAUI TGTACA 1 cut(s) 2281
BstBAI YACGTR 1 cut(s) 554
BstBI TTCGAA 1 cut(s) 34
BstC8I GCNNGC 1 cut(s) 1732
BstDEI CTNAG 7 cut(s) 227, 862, 930, 1407, 1645, 1752, 2292
BstDSI CCRYGG 1 cut(s) 1311
BstENI CCTNNNNNAGG 1 cut(s) 2051
BstF5I GGATG 9 cut(s) 593, 967, 1134, 1639, 2125, 2371, 2434, 2531, 2899
BstFNI CGCG 1 cut(s) 2440
BstH2I RGCGCY 1 cut(s) 2813
BstHHI GCGC 2 cut(s) 2812, 2908
BstMAI GTCTC 5 cut(s) 18, 592, 874, 881, 1538
BstMCI CGRYCG 1 cut(s) 1430
BstMWI GCNNNNNNNGC 7 cut(s) 377, 431, 443, 1521, 1999, 2153, 2912
BstNI CCWGG 6 cut(s) 117, 518, 1630, 2054, 2362, 3093
BstNSI RCATGY 3 cut(s) 182, 2317, 3014
BstSCI CCNGG 8 cut(s) 115, 516, 522, 1424, 1628, 2052, 2360, 3091
BstSFI CTRYAG 4 cut(s) 432, 615, 1569, 2028
BstSLI GKGCMC 1 cut(s) 1995
BstUI CGCG 1 cut(s) 2440
BstV2I GAAGAC 6 cut(s) 23, 54, 448, 570, 1803, 2990
BstXI CCANNNNNNTGG 1 cut(s) 323
Bsu36I CCTNAGG 1 cut(s) 1407
BsuI GTATCC 2 cut(s) 1634, 1878
BsuRI GGCC 6 cut(s) 425, 1074, 1797, 1969, 2530, 2643
BtgI CCRYGG 1 cut(s) 1311
BtsCI GGATG 9 cut(s) 593, 967, 1134, 1639, 2125, 2371, 2434, 2531, 2899
BtsI GCAGTG 1 cut(s) 3102
BveI ACCTGC 2 cut(s) 646, 1725
Cac8I GCNNGC 1 cut(s) 1732
CciI TCATGA 2 cut(s) 745, 2962
CfoI GCGC 2 cut(s) 2812, 2908
Cfr13I GGNCC 7 cut(s) 520, 1021, 1796, 2043, 2228, 2956, 3053
CseI GACGC 2 cut(s) 2429, 2446
CspCI CAANNNNNGTGG 2 cut(s) 44, 79
DdeI CTNAG 7 cut(s) 227, 862, 930, 1407, 1645, 1752, 2292
DraI TTTAAA 1 cut(s) 1215
DraIII CACNNNGTG 1 cut(s) 554
Eam1104I CTCTTC 2 cut(s) 63, 1503
EarI CTCTTC 2 cut(s) 63, 1503
Ecl136II GAGCTC 1 cut(s) 313
Eco130I CCWWGG 3 cut(s) 426, 675, 2922
Eco24I GRGCYC 3 cut(s) 315, 2917, 3101
Eco31I GGTCTC 3 cut(s) 18, 874, 881
Eco32I GATATC 1 cut(s) 507
Eco47I GGWCC 6 cut(s) 520, 1021, 2043, 2228, 2956, 3053
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 5 cut(s) 1527, 2190, 2529, 2640, 2879
Eco72I CACGTG 1 cut(s) 554
Eco81I CCTNAGG 1 cut(s) 1407
Eco88I CYCGRG 2 cut(s) 884, 3039
EcoICRI GAGCTC 1 cut(s) 313
EcoNI CCTNNNNNAGG 1 cut(s) 2051
EcoO109I RGGNCCY 5 cut(s) 520, 1021, 2043, 2228, 2956
EcoRI GAATTC 2 cut(s) 1319, 1640
EcoRII CCWGG 6 cut(s) 115, 516, 1628, 2052, 2360, 3091
EcoRV GATATC 1 cut(s) 507
EcoT14I CCWWGG 3 cut(s) 426, 675, 2922
EcoT22I ATGCAT 2 cut(s) 184, 913
EcoT38I GRGCYC 3 cut(s) 315, 2917, 3101
ErhI CCWWGG 3 cut(s) 426, 675, 2922
Esp3I CGTCTC 1 cut(s) 592
FalI AAGNNNNNCTT 2 cut(s) 845, 877
FaqI GGGAC 5 cut(s) 189, 1007, 1408, 1630, 2214
FauI CCCGC 1 cut(s) 1372
FauNDI CATATG 1 cut(s) 1477
FbaI TGATCA 1 cut(s) 2667
FblI GTMKAC 1 cut(s) 2027
FokI GGATG 9 cut(s) 600, 974, 1121, 1646, 2132, 2378, 2441, 2538, 2906
FriOI GRGCYC 3 cut(s) 315, 2917, 3101
FspBI CTAG 3 cut(s) 1017, 1176, 1916
GlaI GCGC 2 cut(s) 2811, 2907
GsaI CCCAGC 1 cut(s) 2394
GsuI CTGGAG 4 cut(s) 318, 414, 2543, 2694
HaeII RGCGCY 1 cut(s) 2813
HaeIII GGCC 6 cut(s) 425, 1074, 1797, 1969, 2530, 2643
HapII CCGG 7 cut(s) 270, 447, 524, 700, 1426, 1521, 2106
HgaI GACGC 2 cut(s) 2429, 2446
HhaI GCGC 2 cut(s) 2812, 2908
Hin1I GRCGYC 2 cut(s) 1770, 1952
Hin6I GCGC 2 cut(s) 2810, 2906
HinP1I GCGC 2 cut(s) 2810, 2906
HincII GTYRAC 1 cut(s) 1131
HindII GTYRAC 1 cut(s) 1131
HindIII AAGCTT 3 cut(s) 197, 573, 2606
HinfI GANTC 7 cut(s) 1546, 1582, 1618, 1652, 1664, 2210, 3079
HpaII CCGG 7 cut(s) 270, 447, 524, 700, 1426, 1521, 2106
HphI GGTGA 5 cut(s) 178, 1080, 1276, 1445, 2021
Hpy166II GTNNAC 6 cut(s) 705, 1131, 1598, 2028, 2035, 2284
Hpy8I GTNNAC 6 cut(s) 705, 1131, 1598, 2028, 2035, 2284
HpyCH4III ACNGT 9 cut(s) 29, 63, 321, 500, 1312, 1659, 2032, 2405, 2768
HpyCH4IV ACGT 5 cut(s) 553, 601, 875, 1770, 1952
HpyF10VI GCNNNNNNNGC 7 cut(s) 377, 431, 443, 1521, 1999, 2153, 2912
HpyF3I CTNAG 7 cut(s) 227, 862, 930, 1407, 1645, 1752, 2292
HpySE526I ACGT 5 cut(s) 553, 601, 875, 1770, 1952
Hsp92I GRCGYC 2 cut(s) 1770, 1952
HspAI GCGC 2 cut(s) 2810, 2906
KflI GGGWCCC 1 cut(s) 1021
Ksp22I TGATCA 1 cut(s) 2667
LmnI GCTCC 6 cut(s) 318, 395, 938, 1189, 1411, 3096
LweI GCATC 8 cut(s) 916, 1171, 1257, 2068, 2092, 2113, 2530, 3025
MaeI CTAG 3 cut(s) 1017, 1176, 1916
MaeII ACGT 5 cut(s) 553, 601, 875, 1770, 1952
MaeIII GTNAC 7 cut(s) 1086, 1162, 1653, 2140, 2659, 2768, 2831
MfeI CAATTG 2 cut(s) 2058, 2901
MhlI GDGCHC 6 cut(s) 246, 315, 1566, 1995, 2917, 3101
MluI ACGCGT 1 cut(s) 2438
MlyI GAGTC 4 cut(s) 1540, 1612, 1661, 2204
MmeI TCCRAC 2 cut(s) 340, 1980
Mph1103I ATGCAT 2 cut(s) 184, 913
MroXI GAANNNNTTC 4 cut(s) 161, 629, 768, 857
MslI CAYNNNNRTG 4 cut(s) 272, 350, 797, 2718
MspCI CTTAAG 1 cut(s) 1835
MspI CCGG 7 cut(s) 270, 447, 524, 700, 1426, 1521, 2106
MspR9I CCNGG 8 cut(s) 117, 518, 524, 1426, 1630, 2054, 2362, 3093
MunI CAATTG 2 cut(s) 2058, 2901
Mva1269I GAATGC 3 cut(s) 182, 279, 1097
MvaI CCWGG 6 cut(s) 117, 518, 1630, 2054, 2362, 3093
MvnI CGCG 1 cut(s) 2440
MwoI GCNNNNNNNGC 7 cut(s) 377, 431, 443, 1521, 1999, 2153, 2912
NciI CCSGG 2 cut(s) 524, 1426
NdeI CATATG 1 cut(s) 1477
NlaIV GGNNCC 9 cut(s) 522, 940, 1022, 1023, 1729, 1994, 2045, 2230, 3098
NmeAIII GCCGAG 1 cut(s) 1528
NmuCI GTSAC 6 cut(s) 1086, 1162, 1653, 2140, 2768, 2831
NsiI ATGCAT 2 cut(s) 184, 913
NspI RCATGY 3 cut(s) 182, 2317, 3014
NspV TTCGAA 1 cut(s) 34
PagI TCATGA 2 cut(s) 745, 2962
PasI CCCWGGG 1 cut(s) 1629
PciI ACATGT 1 cut(s) 2313
PctI GAATGC 3 cut(s) 182, 279, 1097
PdmI GAANNNNTTC 4 cut(s) 161, 629, 768, 857
PfeI GAWTC 3 cut(s) 1582, 1664, 3079
PflFI GACNNNGTC 1 cut(s) 2252
PflMI CCANNNNNTGG 3 cut(s) 554, 725, 3092
PfoI TCCNGGA 1 cut(s) 516
PleI GAGTC 4 cut(s) 1540, 1612, 1660, 2204
PmaCI CACGTG 1 cut(s) 554
PmlI CACGTG 1 cut(s) 554
PpsI GAGTC 4 cut(s) 1540, 1612, 1660, 2204
Ppu21I YACGTR 1 cut(s) 554
PpuMI RGGWCCY 5 cut(s) 520, 1021, 2043, 2228, 2956
PscI ACATGT 1 cut(s) 2313
Psp124BI GAGCTC 1 cut(s) 315
Psp1406I AACGTT 1 cut(s) 875
Psp5II RGGWCCY 5 cut(s) 520, 1021, 2043, 2228, 2956
Psp6I CCWGG 6 cut(s) 115, 516, 1628, 2052, 2360, 3091
PspCI CACGTG 1 cut(s) 554
PspFI CCCAGC 1 cut(s) 2390
PspGI CCWGG 6 cut(s) 115, 516, 1628, 2052, 2360, 3091
PspN4I GGNNCC 9 cut(s) 522, 940, 1022, 1023, 1729, 1994, 2045, 2230, 3098
PspPI GGNCC 7 cut(s) 520, 1021, 1796, 2043, 2228, 2956, 3053
PspPPI RGGWCCY 5 cut(s) 520, 1021, 2043, 2228, 2956
PstI CTGCAG 1 cut(s) 436
PsyI GACNNNGTC 1 cut(s) 2252
RseI CAYNNNNRTG 4 cut(s) 272, 350, 797, 2718
SacI GAGCTC 1 cut(s) 315
Sau96I GGNCC 7 cut(s) 520, 1021, 1796, 2043, 2228, 2956, 3053
ScaI AGTACT 2 cut(s) 1013, 1964
SchI GAGTC 4 cut(s) 1540, 1612, 1661, 2204
ScrFI CCNGG 8 cut(s) 117, 518, 524, 1426, 1630, 2054, 2362, 3093
SduI GDGCHC 6 cut(s) 246, 315, 1566, 1995, 2917, 3101
SfaNI GCATC 8 cut(s) 916, 1171, 1257, 2068, 2092, 2113, 2530, 3025
SfcI CTRYAG 4 cut(s) 432, 615, 1569, 2028
SfuI TTCGAA 1 cut(s) 34
SinI GGWCC 6 cut(s) 520, 1021, 2043, 2228, 2956, 3053
SmiMI CAYNNNNRTG 4 cut(s) 272, 350, 797, 2718
SmlI CTYRAG 3 cut(s) 200, 1835, 2330
SmoI CTYRAG 3 cut(s) 200, 1835, 2330
SsiI CCGC 5 cut(s) 213, 291, 1379, 2164, 2385
SspI AATATT 1 cut(s) 1862
SspMI CTAG 3 cut(s) 1017, 1176, 1916
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 8 cut(s) 115, 516, 522, 1424, 1628, 2052, 2360, 3091
StyI CCWWGG 3 cut(s) 426, 675, 2922
TaaI ACNGT 9 cut(s) 29, 63, 321, 500, 1312, 1659, 2032, 2405, 2768
TaiI ACGT 5 cut(s) 556, 604, 878, 1773, 1955
TaqI TCGA 4 cut(s) 34, 723, 1047, 2848
TaqII GACCGA 1 cut(s) 2984
TatI WGTACW 4 cut(s) 1011, 1962, 2281, 2370
TauI GCSGC 3 cut(s) 215, 293, 2167
TfiI GAWTC 3 cut(s) 1582, 1664, 3079
TseFI GTSAC 6 cut(s) 1086, 1162, 1653, 2140, 2768, 2831
Tsp45I GTSAC 6 cut(s) 1086, 1162, 1653, 2140, 2768, 2831
TspGWI ACGGA 2 cut(s) 974, 2319
Tth111I GACNNNGTC 1 cut(s) 2252
Van91I CCANNNNNTGG 3 cut(s) 554, 725, 3092
Vha464I CTTAAG 1 cut(s) 1835
VpaK11BI GGWCC 6 cut(s) 520, 1021, 2043, 2228, 2956, 3053
XagI CCTNNNNNAGG 1 cut(s) 2051
XapI RAATTY 7 cut(s) 136, 827, 1319, 1640, 2066, 2128, 2754
XceI RCATGY 3 cut(s) 182, 2317, 3014
XmiI GTMKAC 1 cut(s) 2027
XmnI GAANNNNTTC 4 cut(s) 161, 629, 768, 857
XspI CTAG 3 cut(s) 1017, 1176, 1916
ZraI GACGTC 2 cut(s) 1771, 1953
ZrmI AGTACT 2 cut(s) 1013, 1964
Zsp2I ATGCAT 2 cut(s) 184, 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.