Rh4CG426500

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000598
Physical Location & Seq
Reverse (-)
66907610 .. 66914028
6419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG426500.1

Sequence Viewer

Length: 4359 bp
ATGACTGGCTATGATTTGATTGGCCTTCCATTGATGTCTCCACTTGCACTCAATCAGATCGTTTATGCCCTTCCTATGTTGACCGTCCTAACAGACAAAGGTACTGGGATATTGACTAGTGTACCTAGTGACGCTCCTGATGATTATATGGCCTTGGAAGATTTAAAATTGAAACCAGCTTTTAGAGAAAAATATGGTGTGAAGGATGAATGGGTCATGCCCTTTGATATCATTCCAATAATCAAAATTCCGGAATATGGAGACAAGGCTGCTAAAAAGGTTGTGGAAGATCTCAAAATCAAAAGTCAGAATGAGATAGAGAAGCTAGCAGAAGCCAAGAGGTTGACATCCTTGAAAGGTTTAACTGAAGGAAAACTGCTTGTGGGAGAATTCAAAGAAAGGATAGTCCAGGAGGCAAAACCATTGATAAGGAGCAGGCTAATAGAGGCAGGTGATGCAATCATGTATAGTGAGCCTGAAAAGCAGGTAGTTTCACGATCTGGTGATGAATGTGTTGTTGCTCTTATAGATCAATGGTGCATCACATATGGGGAAGCAGAATGGAAAAAAACTGCTCAGGAGTGCTTGTCATGCATAAATTTATATTCTGACACGACACGACATGGATTTGTACACACATTGAATTGGTTGAATCAGTGGGCTTGTTCTCGATCTTTTGGTCTTGGTACACGCATTCCCTGGGATGAAGAATACTTGGTCGAGCCGTTATCCGATTCAACTCTTTACATGGCTTATTACACCGTTGCTCACCTGTTACACAATGGAGACATGTATGGCAGCTCCAACAGCACATCTGCAGTAAGAGCTGAACAAATGACCGATGAGGTTTGGGATTTTATTTTCTGTGATGGCTCGTATCCACAATCATCTGATATCACACCATCAATTCTTAACAAGATGAAGCAGGAGTTTGAATATTGGTATCCTTTTGATCTTGAGGTGTCTGGTAAGGATCTAATCCAGAATCATACCTTCTGCATTTACAACCATACTGCACTTCTGCGCAAGAAGCACTGGCCTAGTGCGTTTAGATGCAATGGGCACCTGATGCTCAAATCCACGAAGAATGCTAATGGTGCTGGAAATTTTATATCATTGAGACACGCAATTGATCACTATTCTGCTGATGCCACGCGGTTTGCTTTGGCTGATATTAGTGATAGTATTGACGATGCATACTTTTCATTTGAAACTGCAAATGCCGCCATCATACGTCTCACTAAGGAGATAGATTGGATTGATCGGATGAAAGATAAAGATTTGGGTAAAGACTCTTCTCTGAGAATAGGTTCCCCTTGTACCTTCTCTGACCGGGTCTTTCTGAATGAAATAAATATTGCTGTGAAGAGGACTGAGCAAAATTACCAGGGTTGCATGTTCAGGGAAGCCCTAAAGACTGGCTTTTATGATCTTCAAGCTGCCAGGGATTGGTACAGGATTTCATGTGGTGGTACTAATGCAATGAACCACGATTTGGTGTGGCGTTTTATAGATGTGCAGACCCGTCTTATTGCTCCAATTTGTCCCCACTATGCAGAACATGTCTGGAGGGAACTTTTAGAGAGGGAAGGATTTGCAGTAAATGCAGGCTGGCCCGCGGCTGATGATCCAGATCTAACACTCCAGAGTGCGAATAAGTATTTGCAAGACTCAATTGATTCAATAAGGAAGCAGCGTGATAAACAGCTATCAACTTCAAAGAAAACCAATATGGGTGCTTCAGCTACTTCTTTGACAGAAGCGTTGATATATGTTAAGGAGAAATATGACGGATGGAAAGCAGAATGCTTAAGAATACTTCAACATGACTTAAATAGAGATACTCTTACTTCTGCTCAAGAGGACAGGGAGATATTTGAGGCATTGAAGGCTAGTTCTTTTGGTCAAGATGATGATTTTAGCCAAAAGAATTGTATGGAATTTTTGAAGTTCAAGAAGGAGAAGGCAGTTGCACTTGGGGTTCAGGCATTGGACTTGAAGCTACCTTTCGGAGAGATTGAAGTCCTTGAACAGAACTTGGACTTGATTAAAAAACAAATACATGGTCTTGAAGAGGTACAAGTATTGTCAGCTATCAATGCTGCTGATATTGCTAGAGCTGGTCCTCATGCTAGACTGCTAGAGAAGAATCCTCCCTCTCCTGGAAGCCCAACTACGGTATCCAAAGGTGATAACATTCTCCTAGAGATTAAGATTCTCCTAGAGATTGAAGCAGAGGTTCGAGAATGGTGGGAAGAGAAAGGCGTTTTTGTCAATGCTGATCAATCTCCTGACAACCCTCACTATCCTAACGATGAACTGGGGGATGATCGGAAGAAGTGGTTTGAAAACTATCTGTTTGGCGGTTTATCGCGACGGGCATTCCTGATATCTAAGCTAGAGTGTTGTGCAGCTTATCATAGATTAAGAGATGACAATGTGCCATGGCAAATCAAAGCCTCAGGCGCAGACGAACTTCCGGAGAAGATTATAAATCTGTTGGTTGGAGAACCACCAGTATTTCCAACAGTTTGGAGAGCTGCCTATTCTACTGATGCCATGAGACTCTCCTTAGCTTGTTCTTGTGATGGTGATGATTCTCCGAGAATTTTATTTAATACTGCAAATTCTGCTATCCGAAAGCTCACTAAAGAAATGTCATGGATTAGTGAACAACTAGCTTATATGGCTGCTGCTGCAGACTCTTCTTCTTTTGTGAGAAGAATCAGAAGAGATGGCCCCCCCAGTACTTTTGCGGACAAGGTGTTTGCGAATGAGATAAATATTGCTGTGCATAACAGTGATAACTATTACCGGGCTTGCATGTTTCAAGAAGCCCTCATTTCTGGCTTTCTTCAACTTCAGGCTGCCAGGGACTCGTATAGAATTTCATGTGGTCCTCATGGCATGAACCATGATTTGGTGCTGCGCTATATTGATGTGCATACACGTCTAATTGCTCCAATCTGTCCACACTATGCTGAATATGTCTGGCGGGAATATTTAAAAAAGGAGGGGTTTGTGGTAATTAAGTCAGGCTGGCCTGCCGCTGATGCCCCGGATTTGATACTCCAAAGTGCCAATAAGTATTTGCAAGATCTAATTGAACTTATGAGGGAGCTTGAAAAGAAGTTTCGAGCTTTAAATGATGGTAATTATGATGTAACAGAAGAAGAAACGAAGGCAGTAGGTTTGATATATGTTAAGGAGGAAATTGATGGATGGGAGGGTAAGTGCTTGAGGATACTTCAAGATAACTTTGACAGCGAGACAAACACCTTTTCTGCTCAGGACGAGGACATGCTAGATGCATTAATATCGTACATATATGGGGATTTTACCATTCGTCAGGATATAGATTATAGGCAAACTGAACAGCTCTGTATGTCTTTTCTGAAGTTGAAGAAGGATGAAGCACTGAATTTTGGGGCTGATGCCTTAGAGTTGAAGCTACCATTTGGAGAGATTGAGGTCGTTCAAGAAAACTTGGACTTGATTAAGAGAGAAATTGGCCTTCATGAGGTTAAGGTACAAGTTTTCTCTGGTACCACCGGTCTTGAAGATTTTACACTGGATAGTCCTCCATCCCCTGGAAGGCCAGCTGCTGCCTTGGTGAGCCGTGACTTCTATGAGAAAATGGAACGTTCTGCTCCCGGAGTATGGGAATTGAAACGTCGTCTTCAGTTATCAGATAAAGTTCTTACTCCCTCGTCTCGTCCTCCCTCTGAGCTATTGTACCAGAAGAAAATGGAACATCCGTTTAAGTTGTTATTCCAGGAGAAAACGGAACATTCTGACCTTGGAGAACAGGAACCGAATGATGAACAATCCCCAGATAATATTCTTGCTCCATTATATCATCCTCCCTATGAGCTATTGTTTCAGGGCTCTTTCAAAAAGGCCAAACGTACTGCTTCTGCCGAGGACAAATGGCTCCTCGTGAACTTGCAATCGACTAAGGAATTCAGCTCACATCTGATTAATCAAGACACCTGGGCTAATGAAGCTGTCTCGAAAATCATCAGCACTAATTTTATCTTCTGGCAGGCATATGATGATTCAAATGAAGTCAAGAAGATTTGCAGATTCTACAACTTGGTATATACACCTGTAGTGCTTATCATTGACCCCATAAATGGTGAGAATATGCGTTCGTGGTATGGAATGGTTCAACCAGAACGTCTGCTGGAGGATCTGTTAGTACCATTTTTGGAAAATAGCCCCAAGGATCACCATCATCCAGGAGAAGAAAGTTCTCAGCCTCAGCCAGAGGAAAGTGAAGATGATTCGGTTGGTGACAAAACTGATGAAGAAGATGAGGAATTGCAGTGGGCATTGGAAGCTTCCATGAAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

1452

Amino Acids

165.41

Weight (kDa)

4.99

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 172 - 399 2.5e-06 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 308 - 407 9.6e-06 tRNA synthetases class I (M)
Anticodon_1 PF08264 444 - 569 2.9e-17 Anticodon-binding domain of tRNA ligase
Anticodon_1 PF08264 920 - 1045 2.1e-10 Anticodon-binding domain of tRNA ligase
Thioredoxin_7 PF13899 1297 - 1377 3.1e-12 Thioredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2492
AarI CACCTGC 1 cut(s) 440
Acc16I TGCGCA 1 cut(s) 1025
Acc36I ACCTGC 2 cut(s) 440, 475
Acc65I GGTACC 1 cut(s) 3545
AccB1I GGYRCC 2 cut(s) 1062, 3545
AccB7I CCANNNNNTGG 4 cut(s) 1449, 1495, 2920, 3590
AccII CGCG 3 cut(s) 1156, 1619, 2374
AccIII TCCGGA 2 cut(s) 250, 2479
AciI CCGC 8 cut(s) 1156, 1224, 1617, 1619, 2364, 2756, 2995, 3048
AclI AACGTT 1 cut(s) 3643
AclWI GGATC 4 cut(s) 981, 1622, 4200, 4236
AcuI CTGAAG 5 cut(s) 387, 1725, 2846, 3416, 3665
AflII CTTAAG 1 cut(s) 1810
AflIII ACRYGT 3 cut(s) 789, 1561, 2948
AgeI ACCGGT 1 cut(s) 3551
AhlI ACTAGT 1 cut(s) 116
AjiI CACGTC 1 cut(s) 2951
AjuI GAANNNNNNNTTGG 4 cut(s) 1478, 1510, 2903, 2935
Alw26I GTCTC 9 cut(s) 42, 255, 780, 1114, 1241, 2557, 3263, 3717, 4015
AlwI GGATC 4 cut(s) 981, 1622, 4200, 4236
AlwNI CAGNNNCTG 1 cut(s) 3605
Aor13HI TCCGGA 2 cut(s) 250, 2479
AoxI GGCC 9 cut(s) 22, 150, 1037, 1613, 2737, 3041, 3511, 3596, 3900
ArsI GACNNNNNNTTYG 4 cut(s) 2050, 2082, 2750, 2782
AseI ATTAAT 2 cut(s) 3314, 3981
AsiGI ACCGGT 1 cut(s) 3551
Asp700I GAANNNNTTC 1 cut(s) 1309
Asp718I GGTACC 1 cut(s) 3545
AspLEI GCGC 3 cut(s) 1026, 2468, 2931
AspS9I GGNCC 4 cut(s) 1614, 2123, 2738, 2897
AsuC2I CCSGG 4 cut(s) 1334, 2816, 3059, 3654
AsuHPI GGTGA 9 cut(s) 464, 515, 761, 2201, 2603, 3625, 4151, 4223, 4307
AsuNHI GCTAGC 1 cut(s) 325
AvaII GGWCC 2 cut(s) 2123, 2897
AxyI CCTNAGG 1 cut(s) 2461
BaeGI GKGCMC 1 cut(s) 1065
BaeI ACNNNNGTAYC 4 cut(s) 105, 138, 2163, 2196
BanI GGYRCC 2 cut(s) 1062, 3545
BanII GRGCYC 1 cut(s) 3890
BarI GAAGNNNNNNTAC 4 cut(s) 1279, 1311, 2158, 2190
BauI CACGAG 1 cut(s) 3938
BbsI GAAGAC 1 cut(s) 3671
BbvCI CCTCAGC 1 cut(s) 4263
BceAI ACGGC 2 cut(s) 709, 3603
BcgI CGANNNNNNTGC 2 cut(s) 3300, 3334
BciVI GTATCC 4 cut(s) 888, 954, 2191, 3237
BclI TGATCA 2 cut(s) 1132, 2281
BcnI CCSGG 4 cut(s) 1334, 2816, 3059, 3654
BcoDI GTCTC 9 cut(s) 42, 255, 780, 1114, 1241, 2557, 3263, 3717, 4015
BcuI ACTAGT 1 cut(s) 116
BfmI CTRYAG 3 cut(s) 816, 2697, 4110
BfrI CTTAAG 1 cut(s) 1810
BfuAI ACCTGC 2 cut(s) 440, 475
BfuI GTATCC 4 cut(s) 888, 954, 2191, 3237
BglII AGATCT 3 cut(s) 289, 1633, 3097
BmcAI AGTACT 1 cut(s) 2749
Bme18I GGWCC 2 cut(s) 2123, 2897
BmgBI CACGTC 1 cut(s) 2951
BmgT120I GGNCC 4 cut(s) 1614, 2123, 2738, 2897
BmiI GGNNCC 6 cut(s) 1064, 1312, 2740, 3547, 3813, 3935
BmrI ACTGGG 3 cut(s) 114, 2330, 2739
BmtI GCTAGC 1 cut(s) 329
BmuI ACTGGG 3 cut(s) 114, 2330, 2739
BpiI GAAGAC 1 cut(s) 3671
BpmI CTGGAG 3 cut(s) 1588, 1628, 4208
Bpu10I CCTNAGC 4 cut(s) 576, 2572, 3288, 4263
BpuEI CTTGAG 3 cut(s) 977, 1842, 3259
BpuMI CCSGG 4 cut(s) 1334, 2816, 3059, 3654
BsaBI GATNNNNATC 2 cut(s) 1632, 4233
BsaWI WCCGGW 3 cut(s) 250, 2479, 3551
BsaXI ACNNNNNCTCC 6 cut(s) 572, 602, 3218, 3248, 4181, 4211
Bse118I RCCGGY 1 cut(s) 3551
Bse1I ACTGG 8 cut(s) 10, 109, 1040, 1423, 2325, 2516, 2745, 3576
Bse21I CCTNAGG 1 cut(s) 2461
Bse3DI GCAATG 2 cut(s) 1063, 1488
Bse8I GATNNNNATC 2 cut(s) 1632, 4233
BseAI TCCGGA 2 cut(s) 250, 2479
BseJI GATNNNNATC 2 cut(s) 1632, 4233
BseMI GCAATG 2 cut(s) 1063, 1488
BseMII CTCAG 8 cut(s) 590, 1292, 1365, 2475, 3302, 3717, 4271, 4277
BseNI ACTGG 8 cut(s) 10, 109, 1040, 1423, 2325, 2516, 2745, 3576
BseRI GAGGAG 1 cut(s) 3926
BseSI GKGCMC 1 cut(s) 1065
BsgI GTGCAG 3 cut(s) 999, 1538, 2430
Bsh1236I CGCG 3 cut(s) 1156, 1619, 2374
BshFI GGCC 9 cut(s) 24, 152, 1039, 1615, 2739, 3043, 3513, 3598, 3902
BshNI GGYRCC 2 cut(s) 1062, 3545
BshTI ACCGGT 1 cut(s) 3551
BsiSI CCGG 7 cut(s) 251, 1333, 2480, 2815, 3059, 3552, 3654
BslFI GGGAC 2 cut(s) 1530, 2888
BsmAI GTCTC 9 cut(s) 42, 255, 780, 1114, 1241, 2557, 3263, 3717, 4015
BsmBI CGTCTC 2 cut(s) 1241, 3717
BsmFI GGGAC 2 cut(s) 1530, 2888
BsmI GAATGC 4 cut(s) 693, 1093, 1811, 2381
BsnI GGCC 9 cut(s) 24, 152, 1039, 1615, 2739, 3043, 3513, 3598, 3902
Bsp1286I GDGCHC 2 cut(s) 1065, 3890
Bsp13I TCCGGA 2 cut(s) 250, 2479
Bsp1407I TGTACA 1 cut(s) 631
Bsp19I CCATGG 1 cut(s) 2444
Bsp68I TCGCGA 1 cut(s) 2374
BspACI CCGC 8 cut(s) 1156, 1224, 1617, 1619, 2364, 2756, 2995, 3048
BspANI GGCC 9 cut(s) 24, 152, 1039, 1615, 2739, 3043, 3513, 3598, 3902
BspCNI CTCAG 8 cut(s) 589, 1293, 1366, 2474, 3301, 3718, 4270, 4276
BspEI TCCGGA 2 cut(s) 250, 2479
BspFNI CGCG 3 cut(s) 1156, 1619, 2374
BspHI TCATGA 1 cut(s) 3517
BspLI GGNNCC 6 cut(s) 1064, 1312, 2740, 3547, 3813, 3935
BspMAI CTGCAG 2 cut(s) 820, 2701
BspMI ACCTGC 2 cut(s) 440, 475
BspOI GCTAGC 1 cut(s) 329
BspPI GGATC 4 cut(s) 981, 1622, 4200, 4236
BspT107I GGYRCC 2 cut(s) 1062, 3545
BspTI CTTAAG 1 cut(s) 1810
BsrDI GCAATG 2 cut(s) 1063, 1488
BsrFI RCCGGY 1 cut(s) 3551
BsrGI TGTACA 1 cut(s) 631
BsrI ACTGG 8 cut(s) 10, 109, 1040, 1423, 2325, 2516, 2745, 3576
BssAI RCCGGY 1 cut(s) 3551
BssSI CACGAG 1 cut(s) 3938
BssT1I CCWWGG 5 cut(s) 153, 2444, 3609, 3799, 4224
Bst2BI CACGAG 1 cut(s) 3938
Bst4CI ACNGT 5 cut(s) 85, 763, 2179, 2530, 2801
Bst6I CTCTTC 6 cut(s) 1300, 1361, 2067, 2250, 2710, 2725
BstAFI CTTAAG 1 cut(s) 1810
BstAPI GCANNNNNTGC 4 cut(s) 455, 1069, 1604, 2630
BstAUI TGTACA 1 cut(s) 631
BstDSI CCRYGG 2 cut(s) 1617, 2444
BstENI CCTNNNNNAGG 1 cut(s) 3518
BstFNI CGCG 3 cut(s) 1156, 1619, 2374
BstHHI GCGC 3 cut(s) 1026, 2468, 2931
BstMAI GTCTC 9 cut(s) 42, 255, 780, 1114, 1241, 2557, 3263, 3717, 4015
BstNSI RCATGY 5 cut(s) 793, 1399, 1565, 2827, 3304
BstSFI CTRYAG 3 cut(s) 816, 2697, 4110
BstSLI GKGCMC 1 cut(s) 1065
BstUI CGCG 3 cut(s) 1156, 1619, 2374
BstV2I GAAGAC 1 cut(s) 3671
BstX2I RGATCY 5 cut(s) 289, 973, 1633, 3097, 4192
BstXI CCANNNNNNTGG 1 cut(s) 2532
BstYI RGATCY 5 cut(s) 289, 973, 1633, 3097, 4192
Bsu36I CCTNAGG 1 cut(s) 2461
BsuI GTATCC 4 cut(s) 888, 954, 2191, 3237
BsuRI GGCC 9 cut(s) 24, 152, 1039, 1615, 2739, 3043, 3513, 3598, 3902
BtgI CCRYGG 2 cut(s) 1617, 2444
BtrI CACGTC 1 cut(s) 2951
BtsI GCAGTG 1 cut(s) 4334
BtsIMutI CAGTG 6 cut(s) 662, 1033, 2806, 3416, 3569, 4334
BtuMI TCGCGA 1 cut(s) 2374
BveI ACCTGC 2 cut(s) 440, 475
CaiI CAGNNNCTG 1 cut(s) 3605
CciI TCATGA 1 cut(s) 3517
CfoI GCGC 3 cut(s) 1026, 2468, 2931
Cfr10I RCCGGY 1 cut(s) 3551
Cfr13I GGNCC 4 cut(s) 1614, 2123, 2738, 2897
Cfr42I CCGCGG 1 cut(s) 1620
CseI GACGC 1 cut(s) 140
CspAI ACCGGT 1 cut(s) 3551
CspCI CAANNNNNGTGG 4 cut(s) 1141, 1176, 3538, 3573
DraI TTTAAA 3 cut(s) 165, 3006, 3144
Eam1104I CTCTTC 6 cut(s) 1300, 1361, 2067, 2250, 2710, 2725
EarI CTCTTC 6 cut(s) 1300, 1361, 2067, 2250, 2710, 2725
Eco130I CCWWGG 5 cut(s) 153, 2444, 3609, 3799, 4224
Eco24I GRGCYC 1 cut(s) 3890
Eco32I GATATC 3 cut(s) 229, 895, 2391
Eco47I GGWCC 2 cut(s) 2123, 2897
Eco57I CTGAAG 5 cut(s) 387, 1725, 2846, 3416, 3665
Eco81I CCTNAGG 1 cut(s) 2461
EcoNI CCTNNNNNAGG 1 cut(s) 3518
EcoRI GAATTC 2 cut(s) 389, 3962
EcoRV GATATC 3 cut(s) 229, 895, 2391
EcoT14I CCWWGG 5 cut(s) 153, 2444, 3609, 3799, 4224
EcoT22I ATGCAT 3 cut(s) 596, 1198, 3313
EcoT38I GRGCYC 1 cut(s) 3890
ErhI CCWWGG 5 cut(s) 153, 2444, 3609, 3799, 4224
Esp3I CGTCTC 2 cut(s) 1241, 3717
FalI AAGNNNNNCTT 2 cut(s) 1406, 1438
FaqI GGGAC 2 cut(s) 1530, 2888
FauI CCCGC 2 cut(s) 1624, 2988
FauNDI CATATG 2 cut(s) 547, 4051
FbaI TGATCA 2 cut(s) 1132, 2281
FriOI GRGCYC 1 cut(s) 3890
FspI TGCGCA 1 cut(s) 1025
GlaI GCGC 3 cut(s) 1025, 2467, 2930
GsuI CTGGAG 3 cut(s) 1588, 1628, 4208
HaeIII GGCC 9 cut(s) 24, 152, 1039, 1615, 2739, 3043, 3513, 3598, 3902
HapII CCGG 7 cut(s) 251, 1333, 2480, 2815, 3059, 3552, 3654
HgaI GACGC 1 cut(s) 140
HhaI GCGC 3 cut(s) 1026, 2468, 2931
Hin6I GCGC 3 cut(s) 1024, 2466, 2929
HinP1I GCGC 3 cut(s) 1024, 2466, 2929
HincII GTYRAC 2 cut(s) 81, 345
HindII GTYRAC 2 cut(s) 81, 345
HindIII AAGCTT 1 cut(s) 4341
HpaII CCGG 7 cut(s) 251, 1333, 2480, 2815, 3059, 3552, 3654
HphI GGTGA 9 cut(s) 464, 515, 761, 2201, 2603, 3625, 4151, 4223, 4307
Hpy166II GTNNAC 8 cut(s) 81, 122, 345, 634, 689, 2672, 2972, 3943
Hpy8I GTNNAC 8 cut(s) 81, 122, 345, 634, 689, 2672, 2972, 3943
Hpy99I CGWCG 2 cut(s) 2379, 3678
HpyCH4III ACNGT 5 cut(s) 85, 763, 2179, 2530, 2801
HpyCH4IV ACGT 6 cut(s) 1234, 2950, 3643, 3673, 3907, 4180
HpySE526I ACGT 6 cut(s) 1234, 2950, 3643, 3673, 3907, 4180
HspAI GCGC 3 cut(s) 1024, 2466, 2929
Kpn2I TCCGGA 2 cut(s) 250, 2479
KpnI GGTACC 1 cut(s) 3549
Ksp22I TGATCA 2 cut(s) 1132, 2281
KspI CCGCGG 1 cut(s) 1620
LmnI GCTCC 9 cut(s) 139, 432, 806, 1540, 2965, 3118, 3655, 3853, 3939
MaeII ACGT 6 cut(s) 1234, 2950, 3643, 3673, 3907, 4180
MaeIII GTNAC 5 cut(s) 128, 774, 3163, 3620, 4295
MfeI CAATTG 2 cut(s) 1128, 1674
MflI RGATCY 5 cut(s) 289, 973, 1633, 3097, 4192
MhlI GDGCHC 2 cut(s) 1065, 3890
MlyI GAGTC 5 cut(s) 1286, 1664, 2559, 2696, 2870
MmeI TCCRAC 3 cut(s) 828, 2485, 2549
Mph1103I ATGCAT 3 cut(s) 596, 1198, 3313
MroI TCCGGA 2 cut(s) 250, 2479
MroXI GAANNNNTTC 1 cut(s) 1309
MslI CAYNNNNRTG 1 cut(s) 2799
MspA1I CMGCKG 3 cut(s) 1619, 3050, 3602
MspCI CTTAAG 1 cut(s) 1810
MspI CCGG 7 cut(s) 251, 1333, 2480, 2815, 3059, 3552, 3654
MunI CAATTG 2 cut(s) 1128, 1674
Mva1269I GAATGC 4 cut(s) 693, 1093, 1811, 2381
MvnI CGCG 3 cut(s) 1156, 1619, 2374
NciI CCSGG 4 cut(s) 1334, 2816, 3059, 3654
NcoI CCATGG 1 cut(s) 2444
NdeI CATATG 2 cut(s) 547, 4051
NheI GCTAGC 1 cut(s) 325
NlaIV GGNNCC 6 cut(s) 1064, 1312, 2740, 3547, 3813, 3935
NmeAIII GCCGAG 1 cut(s) 3946
NmuCI GTSAC 3 cut(s) 128, 3620, 4295
NruI TCGCGA 1 cut(s) 2374
NsbI TGCGCA 1 cut(s) 1025
NsiI ATGCAT 3 cut(s) 596, 1198, 3313
NspI RCATGY 5 cut(s) 793, 1399, 1565, 2827, 3304
PagI TCATGA 1 cut(s) 3517
PaqCI CACCTGC 1 cut(s) 440
PasI CCCWGGG 1 cut(s) 699
PciI ACATGT 2 cut(s) 789, 1561
PctI GAATGC 4 cut(s) 693, 1093, 1811, 2381
PdmI GAANNNNTTC 1 cut(s) 1309
PflFI GACNNNGTC 1 cut(s) 1334
PflMI CCANNNNNTGG 4 cut(s) 1449, 1495, 2920, 3590
PfoI TCCNGGA 5 cut(s) 408, 2161, 3652, 3774, 4240
PinAI ACCGGT 1 cut(s) 3551
PleI GAGTC 5 cut(s) 1286, 1664, 2559, 2696, 2870
PpsI GAGTC 5 cut(s) 1286, 1664, 2559, 2696, 2870
PscI ACATGT 2 cut(s) 789, 1561
PshBI ATTAAT 2 cut(s) 3314, 3981
PsiI TTATAA 1 cut(s) 2492
Psp1406I AACGTT 1 cut(s) 3643
PspN4I GGNNCC 6 cut(s) 1064, 1312, 2740, 3547, 3813, 3935
PspPI GGNCC 4 cut(s) 1614, 2123, 2738, 2897
PstI CTGCAG 2 cut(s) 820, 2701
PstNI CAGNNNCTG 1 cut(s) 3605
PsuI RGATCY 5 cut(s) 289, 973, 1633, 3097, 4192
PsyI GACNNNGTC 1 cut(s) 1334
PvuII CAGCTG 1 cut(s) 3602
RruI TCGCGA 1 cut(s) 2374
RseI CAYNNNNRTG 1 cut(s) 2799
SacII CCGCGG 1 cut(s) 1620
Sau96I GGNCC 4 cut(s) 1614, 2123, 2738, 2897
ScaI AGTACT 1 cut(s) 2749
SchI GAGTC 5 cut(s) 1286, 1664, 2559, 2696, 2870
SduI GDGCHC 2 cut(s) 1065, 3890
SfcI CTRYAG 3 cut(s) 816, 2697, 4110
Sfr303I CCGCGG 1 cut(s) 1620
SgrBI CCGCGG 1 cut(s) 1620
SinI GGWCC 2 cut(s) 2123, 2897
SmiMI CAYNNNNRTG 1 cut(s) 2799
SmlI CTYRAG 4 cut(s) 956, 1810, 1857, 3238
SmoI CTYRAG 4 cut(s) 956, 1810, 1857, 3238
SpeI ACTAGT 1 cut(s) 116
SsiI CCGC 8 cut(s) 1156, 1224, 1617, 1619, 2364, 2756, 2995, 3048
SspI AATATT 5 cut(s) 938, 1357, 2785, 3002, 3841
StyI CCWWGG 5 cut(s) 153, 2444, 3609, 3799, 4224
TaaI ACNGT 5 cut(s) 85, 763, 2179, 2530, 2801
TaiI ACGT 6 cut(s) 1237, 2953, 3646, 3676, 3910, 4183
TaqI TCGA 6 cut(s) 670, 720, 2242, 3136, 3953, 4013
TaqII GACCGA 1 cut(s) 854
TatI WGTACW 2 cut(s) 631, 2747
TauI GCSGC 3 cut(s) 1226, 1622, 3050
TscAI CASTG 6 cut(s) 662, 1040, 2806, 3423, 3576, 4334
TseFI GTSAC 3 cut(s) 128, 3620, 4295
Tsp45I GTSAC 3 cut(s) 128, 3620, 4295
TspGWI ACGGA 3 cut(s) 1806, 3747, 3800
TspRI CASTG 6 cut(s) 662, 1040, 2806, 3423, 3576, 4334
Tth111I GACNNNGTC 1 cut(s) 1334
Van91I CCANNNNNTGG 4 cut(s) 1449, 1495, 2920, 3590
Vha464I CTTAAG 1 cut(s) 1810
VpaK11BI GGWCC 2 cut(s) 2123, 2897
VspI ATTAAT 2 cut(s) 3314, 3981
XagI CCTNNNNNAGG 1 cut(s) 3518
XceI RCATGY 5 cut(s) 793, 1399, 1565, 2827, 3304
XmnI GAANNNNTTC 1 cut(s) 1309
ZrmI AGTACT 1 cut(s) 2749
Zsp2I ATGCAT 3 cut(s) 596, 1198, 3313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.