FvH4_4g33720

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000598
Physical Location & Seq
Reverse (-)
31838374 .. 31842181
3808 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33720.t2

Sequence Viewer

Length: 2256 bp
ATGGACAGTTCTAAATCTTTTTGCAAGAGATCCAAACCTGCTCTAAGGAGACTGGCAGCTGAAGGTGAGAACATTCTCCTAGAGATTAAGATTCTTCTAGAGATTGAAGCCGAGGTTCGAGAATGGTGGGAAGAGAAAGGTATTTTTGTCCATGCTGATCAATCACCTGACAACCCTCACTATCCTAGTGATGATGAACTAGGGAATGATCGGAAGAAGTGGTTTGAAAACTATCTGTTTGGTGATTTATCCCGAAGGGCATTCCTAATCTCTAAGCTAGAGTGTTGTGCAGCTTATCATAGATTAAGAGATGACAATGTGCCATGGAATATCAAAGCCTCAGGCGCAGAGGAACTTCCGGAGAAGATTATACATCTGTTGACTGGAGAACCGCCAGTGTTTCCGACAGTCTGGAGAGCTGCATATTCCACTGATGCCATGAGACTCTCCTTGGCTTCTTCTTGTAATGGTGGTGATTCTCCAAGAATTTTATTTAATACTGCGAATTCTGCTATCCGGAAGCTCACTAAAGAGATGTCATGGATTAGTGAACAACTAGCTTATATGGCTGCTGCTGCAGACTCTTCTTCTTTTGTGAGAAGAATCAGAAGAGATGGCCCCCCAAGTACATTTGCGGACAAGGTGTTTGCGAACGAGATAAATATTGCTGTGCATAACAGTGATAACTATTACAAGGCTTGCATGTTTCAAGAAGCCCTCATTTCTGGCTTTCTTCAACTTCAAGCTGCCAGGGACTGGTATAGGATTTCATGTGGTCCTTGTGGTATGAACCATGACTTGGTGCTGCGCTATATTGATGTGCATACACGTCTAATTGCTCCAATCTGTCCACACTACGCTGAATATGTCTGGCGAGAATATTTAAAAAAGGAGGGGTTCGTGGTAATTAATTCGGGCTGGCCTGCTGCTGATGCCCCGGATTTGATACTCCAAAGTGCCAATAAGTATTTGCAAGATCTAATAGAATTTATGAGGGAGCTTGAAAAGAAATTCCAAGCTTTAGACGATGATAGATATGATGTGACAGAAGAAGAAAAGAAGGCAGTAGGTTTGATATATGTTAAGGAAGAAGTTGATGGATGGGAGGGGAAGTGCTTGAGGATACTCCAAGATAACTTTGACAGCGAGACAAAGACTTTTTCTGCTCAGGACGAGGACATGCTAGATGCATTAATATCGTACATCTATGGTGATTTTACCATTCGTCAGGATATGGATTATAGGCAAACTGAACAACTCTGTGCGTCTTTTCTGAAGTCAAAGAAGGATGAAGCACTTAATTTTGGGGCTGATGCCTTGGAGTTAAAGCTACCATTTGGAGAGATTGAGGTCCTTCAACAAAACTTGGACTTGATAAAGAGAGAAATTGATCTTCACGAGGTTAAGGTGCAAGCCTTTACTGGTACCAACGGTCTTGAAGATTTCACACTGGATAGTCCTCCATCTCCTGGAAGGCCAGCTGCTGCCTTGGTGAGCCGTGACTTCTATGAGAAAATGGAACGTACTGCTCCTGGAGTATGGGAATTCAAACGTCTTCAGTTCTCAGATAGAGTTCTTACTCCCACGTCTCGTCCTGCCTCTGAGCTATTGTACCAGAAGAAAAAGAAACATCCCTTTAAGTTGTTGTTCCAGGAGAAAACGGGACGTTCTAATCTTGGAGAGCAGAAACAGAATGATGATCAGTTACCAGATAAAATTCTTGCTCCATTATATCATCCTCCTTATGAGCTATTGTTTCATGGCTCTTTCAAAAAGGCAAAACGCACTGCTTCTGTCGAGGACAAATGGCTCCTGGTGAACTTGCAATCGACTAAGGAAATCAGCTCACATCTGATTAACCAAGACACCTGGACTAATGAAGCTGTCTCGAAAATCATCAGCACTAATTTTATCTTCTGGCAGGCGTATGATGATTCAAATGAAGTCAGGAAGATTTGCACCTACTACAACGTGCTATATACACCTGTAGTGCTTATCATTGACCCCATAAATGGTGAGAATATGCGCTCGTGGTATGGAATGGTTCAACCAGAATGTCTGCTGAAGGATCTATTAGTACCATTTATGGAAAATAGACCAAAGGATCACCATCATCCAGGAGAAGAAAGTTCTCAACCTCAACCAGAGGAAAGCGAAGATGATTCGGTTGGAGACAAAACTGACGAAGAAGATGAGGAATTGCAGTGGGCATTGGAAGCTTCATTAAAGCATGAAGGTGACTCCATCACAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

752

Amino Acids

86.32

Weight (kDa)

5.02

Isoelectric Point (pI)

56.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anticodon_1 PF08264 213 - 339 1.7e-11 Anticodon-binding domain of tRNA ligase
Thioredoxin_7 PF13899 589 - 669 3.9e-11 Thioredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09620
fragaria_vesca FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_1g25930 FvH4_3g34581 FvH4_4g33720 FvH4_4g33720
malus_domestica MD08G1244600.v1.1 MD15G1439400.v1.1
prunus_persica Prupe.1G582000_v2.0.a1 Prupe.2G015100_v2.0.a1 Prupe.2G123100_v2.0.a1
pyrus_communis pycom08g21210 pycom08g21220 pycom15g38730
rosa_chinensis RchiOBHm_Chr1g0336121 RchiOBHm_Chr1g0353811 RchiOBHm_Chr1g0373331 RchiOBHm_Chr4g0443191 RchiOBHm_Chr4g0443201 RchiOBHm_Chr4g0443211
rosa_laevigata RLG00000003122 RLG00000005948 RLG00000026826 RLG00000028251 RLG00000028252 RLG00000028253 RLG00000029377
rosa_multiflora Rmu_co8002498.1_g000001 Rmu_co8262279.1_g000001 Rmu_co8314139.1_g000001 Rmu_co8393711.1_g000001 Rmu_co8419167.1_g000001 Rmu_sc0007968.1_g000004 Rmu_sc0018919.1_g000004 Rmu_sc0027447.1_g000001
rosa_roxburghii Rroxscaffold_4G00284260 Rroxscaffold_4G00301490 Rroxscaffold_4G00315860 Rroxscaffold_5G00383790 Rroxscaffold_5G00383800
rosa_rugosa Rorug01G0123100 Rorug01G0238700 Rorug01G0377100 Rorug01G0377100 Rorug01G0377200 Rorug01G0377300 Rorug04G0344700 Rorug05G0347900.1
rosa_samantha Rh1AG143800 Rh1AG250300 Rh1AG386300 Rh1BG113400 Rh1BG220800 Rh1BG220900 Rh1BG350400 Rh1CG135500 Rh1CG233100 Rh1CG363500 Rh1DG150200 Rh1DG247700 Rh1DG247800 Rh1DG381300 Rh4AG399400 Rh4AG399500 Rh4BG411000 Rh4BG411100 Rh4BG411200 Rh4CG426500 Rh4CG426600 Rh4DG406100 Rh4DG406200 Rh4DG406300
rosa_wichuraiana Rw0G015190 Rw1G021850 Rw1G034870 Rw4G034440 Rw4G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 46
Acc65I GGTACC 1 cut(s) 1424
AccB1I GGYRCC 1 cut(s) 1424
AccB7I CCANNNNNTGG 3 cut(s) 756, 799, 1469
AccIII TCCGGA 2 cut(s) 358, 516
AciI CCGC 2 cut(s) 392, 635
AclWI GGATC 3 cut(s) 24, 2076, 2112
AcsI RAATTY 6 cut(s) 486, 505, 986, 1010, 1544, 1716
AcuI CTGAAG 4 cut(s) 81, 1295, 1541, 2084
AfaI GTAC 6 cut(s) 628, 1202, 1426, 1525, 1613, 2079
AfiI CCNNNNNNNGG 4 cut(s) 756, 799, 1469, 2012
AflIII ACRYGT 1 cut(s) 827
AjiI CACGTC 2 cut(s) 830, 1587
AjnI CCWGG 7 cut(s) 749, 1468, 1531, 1650, 1812, 1868, 2116
AjuI GAANNNNNNNTTGG 2 cut(s) 782, 814
Alw26I GTCTC 6 cut(s) 43, 436, 1142, 1593, 1891, 2166
AlwI GGATC 3 cut(s) 24, 2076, 2112
AlwNI CAGNNNCTG 2 cut(s) 756, 1484
Aor13HI TCCGGA 2 cut(s) 358, 516
AoxI GGCC 3 cut(s) 616, 920, 1475
ApoI RAATTY 6 cut(s) 486, 505, 986, 1010, 1544, 1716
ArsI GACNNNNNNTTYG 2 cut(s) 629, 661
AseI ATTAAT 2 cut(s) 909, 1193
Asp718I GGTACC 1 cut(s) 1424
AspLEI GCGC 3 cut(s) 347, 810, 2028
AspS9I GGNCC 3 cut(s) 617, 776, 1351
AsuC2I CCSGG 1 cut(s) 938
AvaII GGWCC 2 cut(s) 776, 1351
AxyI CCTNAGG 1 cut(s) 340
BanI GGYRCC 1 cut(s) 1424
BauI CACGAG 2 cut(s) 1397, 2029
BbsI GAAGAC 1 cut(s) 1547
BccI CCATC 6 cut(s) 608, 1091, 1095, 1471, 2118, 2252
BceAI ACGGC 1 cut(s) 1482
BcgI CGANNNNNNTGC 2 cut(s) 1179, 1213
BciT130I CCWGG 7 cut(s) 751, 1470, 1533, 1652, 1814, 1870, 2118
BciVI GTATCC 1 cut(s) 1116
BclI TGATCA 2 cut(s) 157, 1699
BcnI CCSGG 1 cut(s) 938
BcoDI GTCTC 6 cut(s) 43, 436, 1142, 1593, 1891, 2166
BfaI CTAG 7 cut(s) 80, 98, 186, 200, 278, 557, 1184
BfmI CTRYAG 2 cut(s) 576, 1986
BfuAI ACCTGC 1 cut(s) 46
BfuI GTATCC 1 cut(s) 1116
BglII AGATCT 1 cut(s) 976
Bme1390I CCNGG 8 cut(s) 751, 938, 1470, 1533, 1652, 1814, 1870, 2118
Bme18I GGWCC 2 cut(s) 776, 1351
BmgBI CACGTC 2 cut(s) 830, 1587
BmgT120I GGNCC 3 cut(s) 617, 776, 1351
BmiI GGNNCC 3 cut(s) 619, 1426, 1811
BmrFI CCNGG 8 cut(s) 751, 938, 1470, 1533, 1652, 1814, 1870, 2118
BmsI GCATC 4 cut(s) 424, 922, 1177, 1303
BpiI GAAGAC 1 cut(s) 1547
BpmI CTGGAG 3 cut(s) 405, 433, 1554
Bpu10I CCTNAGC 1 cut(s) 1167
BpuEI CTTGAG 1 cut(s) 1138
BpuMI CCSGG 1 cut(s) 938
BsaBI GATNNNNATC 1 cut(s) 2109
BsaJI CCNNGG 7 cut(s) 111, 323, 450, 750, 936, 1317, 1488
BsaWI WCCGGW 2 cut(s) 358, 516
BsaXI ACNNNNNCTCC 2 cut(s) 1097, 1127
Bsc4I CCNNNNNNNGG 4 cut(s) 756, 799, 1469, 2012
Bse1I ACTGG 6 cut(s) 57, 388, 395, 761, 1426, 1455
Bse21I CCTNAGG 1 cut(s) 340
Bse8I GATNNNNATC 1 cut(s) 2109
BseAI TCCGGA 2 cut(s) 358, 516
BseBI CCWGG 7 cut(s) 751, 1470, 1533, 1652, 1814, 1870, 2118
BseDI CCNNGG 7 cut(s) 111, 323, 450, 750, 936, 1317, 1488
BseGI GGATG 5 cut(s) 1106, 1294, 1630, 1735, 2113
BseJI GATNNNNATC 1 cut(s) 2109
BseLI CCNNNNNNNGG 4 cut(s) 756, 799, 1469, 2012
BseMII CTCAG 4 cut(s) 354, 1181, 1578, 1593
BseNI ACTGG 6 cut(s) 57, 388, 395, 761, 1426, 1455
BsgI GTGCAG 1 cut(s) 309
BshFI GGCC 3 cut(s) 618, 922, 1477
BshNI GGYRCC 1 cut(s) 1424
BsiSI CCGG 3 cut(s) 359, 517, 938
BslFI GGGAC 2 cut(s) 767, 1677
BslI CCNNNNNNNGG 4 cut(s) 756, 799, 1469, 2012
BsmAI GTCTC 6 cut(s) 43, 436, 1142, 1593, 1891, 2166
BsmBI CGTCTC 1 cut(s) 1593
BsmFI GGGAC 2 cut(s) 767, 1677
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 3 cut(s) 618, 922, 1477
Bsp13I TCCGGA 2 cut(s) 358, 516
Bsp143I GATC 8 cut(s) 29, 157, 208, 976, 1390, 1699, 2068, 2104
Bsp19I CCATGG 1 cut(s) 323
BspACI CCGC 2 cut(s) 392, 635
BspANI GGCC 3 cut(s) 618, 922, 1477
BspCNI CTCAG 4 cut(s) 353, 1180, 1577, 1594
BspEI TCCGGA 2 cut(s) 358, 516
BspLI GGNNCC 3 cut(s) 619, 1426, 1811
BspMAI CTGCAG 1 cut(s) 580
BspMI ACCTGC 1 cut(s) 46
BspPI GGATC 3 cut(s) 24, 2076, 2112
BspT107I GGYRCC 1 cut(s) 1424
BsrI ACTGG 6 cut(s) 57, 388, 395, 761, 1426, 1455
BssECI CCNNGG 7 cut(s) 111, 323, 450, 750, 936, 1317, 1488
BssMI GATC 8 cut(s) 29, 157, 208, 976, 1390, 1699, 2068, 2104
BssSI CACGAG 2 cut(s) 1397, 2029
BssT1I CCWWGG 4 cut(s) 323, 450, 1317, 1488
Bst2BI CACGAG 2 cut(s) 1397, 2029
Bst2UI CCWGG 7 cut(s) 751, 1470, 1533, 1652, 1814, 1870, 2118
Bst4CI ACNGT 4 cut(s) 8, 409, 680, 1433
Bst6I CTCTTC 3 cut(s) 126, 589, 604
BstC8I GCNNGC 6 cut(s) 700, 920, 924, 1413, 1479, 1923
BstDEI CTNAG 7 cut(s) 44, 273, 340, 1167, 1564, 1602, 1833
BstDSI CCRYGG 1 cut(s) 323
BstF5I GGATG 5 cut(s) 1106, 1294, 1630, 1735, 2113
BstHHI GCGC 3 cut(s) 347, 810, 2028
BstKTI GATC 8 cut(s) 32, 160, 211, 979, 1393, 1702, 2071, 2107
BstMAI GTCTC 6 cut(s) 43, 436, 1142, 1593, 1891, 2166
BstMBI GATC 8 cut(s) 29, 157, 208, 976, 1390, 1699, 2068, 2104
BstMWI GCNNNNNNNGC 6 cut(s) 344, 509, 566, 575, 932, 2216
BstNI CCWGG 7 cut(s) 751, 1470, 1533, 1652, 1814, 1870, 2118
BstNSI RCATGY 2 cut(s) 706, 1183
BstSCI CCNGG 8 cut(s) 749, 936, 1468, 1531, 1650, 1812, 1868, 2116
BstSFI CTRYAG 2 cut(s) 576, 1986
BstV2I GAAGAC 1 cut(s) 1547
BstX2I RGATCY 3 cut(s) 29, 976, 2068
BstYI RGATCY 3 cut(s) 29, 976, 2068
Bsu36I CCTNAGG 1 cut(s) 340
BsuI GTATCC 1 cut(s) 1116
BsuRI GGCC 3 cut(s) 618, 922, 1477
BtgI CCRYGG 1 cut(s) 323
BtrI CACGTC 2 cut(s) 830, 1587
BtsCI GGATG 5 cut(s) 1106, 1294, 1630, 1735, 2113
BtsI GCAGTG 2 cut(s) 1785, 2210
BtsIMutI CAGTG 6 cut(s) 402, 429, 685, 1448, 1785, 2210
BveI ACCTGC 1 cut(s) 46
Cac8I GCNNGC 6 cut(s) 700, 920, 924, 1413, 1479, 1923
CaiI CAGNNNCTG 2 cut(s) 756, 1484
CfoI GCGC 3 cut(s) 347, 810, 2028
Cfr13I GGNCC 3 cut(s) 617, 776, 1351
CseI GACGC 1 cut(s) 1254
Csp6I GTAC 6 cut(s) 627, 1201, 1425, 1524, 1612, 2078
CviQI GTAC 6 cut(s) 627, 1201, 1425, 1524, 1612, 2078
DdeI CTNAG 7 cut(s) 44, 273, 340, 1167, 1564, 1602, 1833
DpnI GATC 8 cut(s) 31, 159, 210, 978, 1392, 1701, 2070, 2106
DpnII GATC 8 cut(s) 29, 157, 208, 976, 1390, 1699, 2068, 2104
DraI TTTAAA 1 cut(s) 885
Eam1104I CTCTTC 3 cut(s) 126, 589, 604
EarI CTCTTC 3 cut(s) 126, 589, 604
Eco130I CCWWGG 4 cut(s) 323, 450, 1317, 1488
Eco47I GGWCC 2 cut(s) 776, 1351
Eco57I CTGAAG 4 cut(s) 81, 1295, 1541, 2084
Eco81I CCTNAGG 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 1351
EcoRI GAATTC 2 cut(s) 505, 1544
EcoRII CCWGG 7 cut(s) 749, 1468, 1531, 1650, 1812, 1868, 2116
EcoT14I CCWWGG 4 cut(s) 323, 450, 1317, 1488
EcoT22I ATGCAT 1 cut(s) 1192
ErhI CCWWGG 4 cut(s) 323, 450, 1317, 1488
Esp3I CGTCTC 1 cut(s) 1593
FaqI GGGAC 2 cut(s) 767, 1677
FbaI TGATCA 2 cut(s) 157, 1699
FokI GGATG 5 cut(s) 1113, 1301, 1617, 1722, 2100
FspBI CTAG 7 cut(s) 80, 98, 186, 200, 278, 557, 1184
GlaI GCGC 3 cut(s) 346, 809, 2027
GsuI CTGGAG 3 cut(s) 405, 433, 1554
HaeIII GGCC 3 cut(s) 618, 922, 1477
HapII CCGG 3 cut(s) 359, 517, 938
HgaI GACGC 1 cut(s) 1254
HhaI GCGC 3 cut(s) 347, 810, 2028
Hin6I GCGC 3 cut(s) 345, 808, 2026
HinP1I GCGC 3 cut(s) 345, 808, 2026
HincII GTYRAC 1 cut(s) 381
HindII GTYRAC 1 cut(s) 381
HindIII AAGCTT 2 cut(s) 1017, 2217
HinfI GANTC 8 cut(s) 91, 444, 476, 581, 603, 1934, 2162, 2240
HpaII CCGG 3 cut(s) 359, 517, 938
Hpy166II GTNNAC 4 cut(s) 381, 551, 851, 1819
Hpy188I TCNGA 7 cut(s) 213, 405, 608, 1275, 1567, 1603, 1854
Hpy8I GTNNAC 4 cut(s) 381, 551, 851, 1819
HpyAV CCTTC 8 cut(s) 56, 249, 1054, 1279, 1364, 1467, 2059, 2228
HpyCH4III ACNGT 4 cut(s) 8, 409, 680, 1433
HpyCH4IV ACGT 6 cut(s) 829, 1522, 1552, 1586, 1666, 1971
HpyF10VI GCNNNNNNNGC 6 cut(s) 344, 509, 566, 575, 932, 2216
HpyF3I CTNAG 7 cut(s) 44, 273, 340, 1167, 1564, 1602, 1833
HpySE526I ACGT 6 cut(s) 829, 1522, 1552, 1586, 1666, 1971
HspAI GCGC 3 cut(s) 345, 808, 2026
Kpn2I TCCGGA 2 cut(s) 358, 516
KpnI GGTACC 1 cut(s) 1428
Ksp22I TGATCA 2 cut(s) 157, 1699
Kzo9I GATC 8 cut(s) 29, 157, 208, 976, 1390, 1699, 2068, 2104
LmnI GCTCC 5 cut(s) 844, 997, 1534, 1729, 1815
LweI GCATC 4 cut(s) 424, 922, 1177, 1303
MaeI CTAG 7 cut(s) 80, 98, 186, 200, 278, 557, 1184
MaeII ACGT 6 cut(s) 829, 1522, 1552, 1586, 1666, 1971
MaeIII GTNAC 4 cut(s) 1042, 1499, 1704, 2237
MalI GATC 8 cut(s) 31, 159, 210, 978, 1392, 1701, 2070, 2106
MboI GATC 8 cut(s) 29, 157, 208, 976, 1390, 1699, 2068, 2104
MflI RGATCY 3 cut(s) 29, 976, 2068
MlyI GAGTC 3 cut(s) 438, 575, 2234
MmeI TCCRAC 2 cut(s) 428, 2149
Mph1103I ATGCAT 1 cut(s) 1192
MroI TCCGGA 2 cut(s) 358, 516
MslI CAYNNNNRTG 2 cut(s) 678, 2235
MspA1I CMGCKG 2 cut(s) 59, 1481
MspI CCGG 3 cut(s) 359, 517, 938
MspR9I CCNGG 8 cut(s) 751, 938, 1470, 1533, 1652, 1814, 1870, 2118
Mva1269I GAATGC 1 cut(s) 260
MvaI CCWGG 7 cut(s) 751, 1470, 1533, 1652, 1814, 1870, 2118
MwoI GCNNNNNNNGC 6 cut(s) 344, 509, 566, 575, 932, 2216
NciI CCSGG 1 cut(s) 938
NcoI CCATGG 1 cut(s) 323
NdeII GATC 8 cut(s) 29, 157, 208, 976, 1390, 1699, 2068, 2104
NlaIV GGNNCC 3 cut(s) 619, 1426, 1811
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 3 cut(s) 1042, 1499, 2237
NsiI ATGCAT 1 cut(s) 1192
NspI RCATGY 2 cut(s) 706, 1183
PctI GAATGC 1 cut(s) 260
PfeI GAWTC 5 cut(s) 91, 476, 603, 1934, 2162
PflMI CCANNNNNTGG 3 cut(s) 756, 799, 1469
PfoI TCCNGGA 4 cut(s) 1468, 1531, 1650, 2116
PleI GAGTC 3 cut(s) 438, 575, 2234
PpsI GAGTC 3 cut(s) 438, 575, 2234
PpuMI RGGWCCY 1 cut(s) 1351
PshBI ATTAAT 2 cut(s) 909, 1193
Psp5II RGGWCCY 1 cut(s) 1351
Psp6I CCWGG 7 cut(s) 749, 1468, 1531, 1650, 1812, 1868, 2116
PspGI CCWGG 7 cut(s) 749, 1468, 1531, 1650, 1812, 1868, 2116
PspN4I GGNNCC 3 cut(s) 619, 1426, 1811
PspPI GGNCC 3 cut(s) 617, 776, 1351
PspPPI RGGWCCY 1 cut(s) 1351
PstI CTGCAG 1 cut(s) 580
PstNI CAGNNNCTG 2 cut(s) 756, 1484
PsuI RGATCY 3 cut(s) 29, 976, 2068
PvuII CAGCTG 2 cut(s) 59, 1481
RsaI GTAC 6 cut(s) 628, 1202, 1426, 1525, 1613, 2079
RsaNI GTAC 6 cut(s) 627, 1201, 1425, 1524, 1612, 2078
RseI CAYNNNNRTG 2 cut(s) 678, 2235
Sau3AI GATC 8 cut(s) 29, 157, 208, 976, 1390, 1699, 2068, 2104
Sau96I GGNCC 3 cut(s) 617, 776, 1351
SchI GAGTC 3 cut(s) 438, 575, 2234
ScrFI CCNGG 8 cut(s) 751, 938, 1470, 1533, 1652, 1814, 1870, 2118
SfaNI GCATC 4 cut(s) 424, 922, 1177, 1303
SfcI CTRYAG 2 cut(s) 576, 1986
SinI GGWCC 2 cut(s) 776, 1351
SmiMI CAYNNNNRTG 2 cut(s) 678, 2235
SmlI CTYRAG 1 cut(s) 1117
SmoI CTYRAG 1 cut(s) 1117
SsiI CCGC 2 cut(s) 392, 635
SspI AATATT 2 cut(s) 664, 881
SspMI CTAG 7 cut(s) 80, 98, 186, 200, 278, 557, 1184
StyD4I CCNGG 8 cut(s) 749, 936, 1468, 1531, 1650, 1812, 1868, 2116
StyI CCWWGG 4 cut(s) 323, 450, 1317, 1488
TaaI ACNGT 4 cut(s) 8, 409, 680, 1433
TaiI ACGT 6 cut(s) 832, 1525, 1555, 1589, 1669, 1974
TaqI TCGA 4 cut(s) 118, 1797, 1829, 1889
TatI WGTACW 1 cut(s) 626
TfiI GAWTC 5 cut(s) 91, 476, 603, 1934, 2162
TscAI CASTG 6 cut(s) 402, 436, 685, 1455, 1792, 2210
TseFI GTSAC 3 cut(s) 1042, 1499, 2237
Tsp45I GTSAC 3 cut(s) 1042, 1499, 2237
TspDTI ATGAA 9 cut(s) 210, 759, 803, 1305, 1748, 1893, 1956, 2211, 2247
TspRI CASTG 6 cut(s) 402, 436, 685, 1455, 1792, 2210
Van91I CCANNNNNTGG 3 cut(s) 756, 799, 1469
VpaK11BI GGWCC 2 cut(s) 776, 1351
VspI ATTAAT 2 cut(s) 909, 1193
XapI RAATTY 6 cut(s) 486, 505, 986, 1010, 1544, 1716
XbaI TCTAGA 1 cut(s) 97
XceI RCATGY 2 cut(s) 706, 1183
XspI CTAG 7 cut(s) 80, 98, 186, 200, 278, 557, 1184
Zsp2I ATGCAT 1 cut(s) 1192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.